##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051276_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11810273 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.069874083350996 31.0 30.0 34.0 16.0 34.0 2 30.32722579740536 33.0 31.0 34.0 19.0 34.0 3 30.835777039192912 33.0 31.0 34.0 25.0 34.0 4 34.686005734160425 37.0 35.0 37.0 32.0 37.0 5 35.28804101310782 37.0 35.0 37.0 33.0 37.0 6 35.584729159097336 37.0 35.0 37.0 33.0 37.0 7 35.815921443983555 37.0 35.0 37.0 35.0 37.0 8 35.72863294523336 37.0 35.0 37.0 35.0 37.0 9 37.59814790056081 39.0 37.0 39.0 35.0 39.0 10-11 37.61301309461686 39.0 37.0 39.0 35.0 39.0 12-13 37.681415196752866 39.0 37.0 39.0 35.0 39.0 14-15 39.030006461323964 40.0 38.0 41.0 36.0 41.0 16-17 38.98752992416009 40.0 38.0 41.0 36.0 41.0 18-19 38.97909849332018 40.0 38.5 41.0 35.5 41.0 20-21 38.943986392185856 40.0 39.0 41.0 35.0 41.0 22-23 38.857577254988094 40.0 38.0 41.0 35.0 41.0 24-25 38.76475505688988 40.0 38.0 41.0 35.0 41.0 26-27 38.6262020361426 40.0 38.0 41.0 34.5 41.0 28-29 38.42603600272407 40.0 38.0 41.0 34.0 41.0 30-31 38.38885002912295 40.0 38.0 41.0 34.0 41.0 32-33 38.21196550663986 40.0 38.0 41.0 34.0 41.0 34-35 38.26843181355757 40.0 38.0 41.0 34.0 41.0 36-37 38.18634912165028 40.0 38.0 41.0 34.0 41.0 38-39 38.069006321869104 40.0 37.5 41.0 33.0 41.0 40-41 37.888802062407876 40.0 37.0 41.0 33.0 41.0 42-43 37.68956090176747 40.0 37.0 41.0 33.0 41.0 44-45 37.62662988400014 40.0 37.0 41.0 33.0 41.0 46-47 37.694124979160094 40.0 37.0 41.0 33.0 41.0 48-49 37.87401692577301 40.0 37.0 41.0 33.0 41.0 50-51 37.84746758182474 40.0 37.0 41.0 33.0 41.0 52-53 37.6819383430002 40.0 36.5 41.0 33.0 41.0 54-55 37.45786384446829 39.5 36.0 41.0 33.0 41.0 56-57 37.180665256425485 39.0 35.5 41.0 32.0 41.0 58-59 36.84506941541487 39.0 35.0 40.5 31.0 41.0 60-61 36.534381847058064 38.5 35.0 40.0 31.0 41.0 62-63 36.15829126049839 38.0 35.0 40.0 31.0 41.0 64-65 35.88825097438476 37.5 35.0 40.0 30.5 41.0 66-67 35.57359287969042 37.0 35.0 39.5 30.0 41.0 68-69 35.16110846887282 36.5 34.0 39.0 30.0 41.0 70-71 34.735285670365116 36.0 34.0 39.0 29.5 40.5 72-73 34.23485058304749 35.0 34.0 38.5 29.0 40.0 74-75 33.82908845544891 35.0 33.5 37.0 29.0 39.0 76-77 32.89556160132793 35.0 32.5 36.0 26.5 39.0 78-79 33.120798731748195 35.0 33.0 36.0 28.0 39.0 80-81 32.954751892695455 35.0 33.0 36.0 28.0 37.5 82-83 32.76082792497684 35.0 33.0 36.0 28.0 37.0 84-85 32.54810739768674 35.0 33.0 35.0 27.5 37.0 86-87 32.29833425527082 35.0 33.0 35.0 27.0 36.5 88-89 32.00224808520514 35.0 33.0 35.0 26.5 36.0 90-91 31.650711926811514 35.0 32.5 35.0 25.5 36.0 92-93 31.29933842342171 35.0 32.0 35.0 24.0 36.0 94-95 31.20915972899187 35.0 32.0 35.0 24.0 35.0 96-97 30.91287203945243 34.5 32.0 35.0 22.5 35.0 98-99 30.661069646738902 34.0 32.0 35.0 20.0 35.0 100-101 30.10052134273272 34.0 31.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.152906501658489 1101 2 3.901185612675846 1101 3 2.679507660176533 1101 4 2.223420064368703 1101 5 1.297090848846345 1101 6 0.9000615018122033 1101 7 0.5214325277087966 1101 8 0.9563826961946305 1101 9 0.8726814289343849 1101 10-11 0.9209360104913884 1101 12-13 0.6038475276347555 1101 14-15 0.6944798244433557 1101 16-17 0.6944334683323703 1101 18-19 0.6178637078108551 1101 20-21 0.6819315361978511 1101 22-23 0.7232771942163083 1101 24-25 0.8432712492806331 1101 26-27 0.7318119716713127 1101 28-29 1.0619215570815044 1101 30-31 0.7257643576067068 1101 32-33 0.8237946075640892 1101 34-35 0.5417153151240157 1101 36-37 0.45483753247173553 1101 38-39 0.42989499381622664 1101 40-41 0.5633615546813786 1101 42-43 0.6758665282579059 1101 44-45 0.5401371690231329 1101 46-47 0.5308151238723227 1101 48-49 0.5330951600975169 1101 50-51 0.5483715436575594 1101 52-53 0.6151642534028738 1101 54-55 0.6695980650975244 1101 56-57 0.633046196166795 1101 58-59 0.62715865293044 1101 60-61 0.7477676211099151 1101 62-63 0.749549491405908 1101 64-65 0.5962351799983026 1101 66-67 0.5532973349295744 1101 68-69 0.524720492631424 1101 70-71 0.5597953660875064 1101 72-73 0.4921503066120181 1101 74-75 0.46669855425908935 1101 76-77 0.5773897969466688 1101 78-79 0.4054730288815023 1101 80-81 0.3460286134858066 1101 82-83 0.4225850074647184 1101 84-85 0.30320919407343183 1101 86-87 0.31649534465669404 1101 88-89 0.506449994612101 1101 90-91 0.6336059239346419 1101 92-93 0.6549216772955866 1101 94-95 0.46196041218095374 1101 96-97 0.715327033422934 1101 98-99 0.8426540978489463 1101 100-101 0.9614038832336149 1102 1 5.205399263261587 1102 2 3.8745489426527087 1102 3 2.725872349814807 1102 4 2.1984921492535676 1102 5 1.2753056599962775 1102 6 0.8792422674920175 1102 7 0.5079148406580245 1102 8 0.9276850996920771 1102 9 0.8996865332288877 1102 10-11 0.9138143218294488 1102 12-13 0.5927551481216113 1102 14-15 0.6668114294193117 1102 16-17 0.7687537828073729 1102 18-19 0.6619225522160406 1102 20-21 0.6456236098625752 1102 22-23 0.7773890228718017 1102 24-25 0.7944758872830278 1102 26-27 0.7021539194253492 1102 28-29 1.0493253856425824 1102 30-31 0.7261521108440476 1102 32-33 0.9448538438193452 1102 34-35 0.5814332835182441 1102 36-37 0.43835857287877644 1102 38-39 0.407617176006859 1102 40-41 0.6196068221422308 1102 42-43 0.779182685313863 1102 44-45 0.6692576761033351 1102 46-47 0.6677544161978801 1102 48-49 0.539558467994496 1102 50-51 0.6015839826964324 1102 52-53 0.5864045128244655 1102 54-55 0.73209994216203 1102 56-57 0.6839749408914813 1102 58-59 0.6308225769207993 1102 60-61 0.6174538524401783 1102 62-63 0.9121756746917953 1102 64-65 0.8402709843323777 1102 66-67 0.6626097675406228 1102 68-69 0.6609650156220113 1102 70-71 0.6848922662324668 1102 72-73 0.6741049860687198 1102 74-75 0.629994760783056 1102 76-77 0.6441243407582533 1102 78-79 0.6433038312028643 1102 80-81 0.5512361811170194 1102 82-83 0.4974869626998455 1102 84-85 0.3537444375375145 1102 86-87 0.38857187697252726 1102 88-89 0.6926773370882877 1102 90-91 0.7309268802691271 1102 92-93 0.6798358189631166 1102 94-95 0.5569817542018853 1102 96-97 0.7430850384160941 1102 98-99 0.9039418895908788 1102 100-101 1.206970135367179 1103 1 5.239111720625022 1103 2 3.910047560051403 1103 3 2.760352391206787 1103 4 2.2857186852824967 1103 5 1.3319175103680294 1103 6 0.9553456932543227 1103 7 0.5375268405517986 1103 8 0.9374575332715551 1103 9 0.9547659059551066 1103 10-11 0.9531541007763451 1103 12-13 0.6275006139283548 1103 14-15 0.6804539825036287 1103 16-17 0.7040130941026135 1103 18-19 0.6474448440354479 1103 20-21 0.7018874798199946 1103 22-23 0.7238796344104017 1103 24-25 0.8442132992106295 1103 26-27 0.7468959126794559 1103 28-29 1.0874738062082585 1103 30-31 0.7876759486051697 1103 32-33 0.9435011448194786 1103 34-35 0.6192734868115082 1103 36-37 0.5388395971477635 1103 38-39 0.5262239368886199 1103 40-41 0.6076360055289385 1103 42-43 0.848346568275339 1103 44-45 0.6573340017462854 1103 46-47 0.5960568066153442 1103 48-49 0.5484265599619391 1103 50-51 0.5059441603678465 1103 52-53 0.5466559084244196 1103 54-55 0.6364614515271185 1103 56-57 0.6841509526606941 1103 58-59 0.6573923579033973 1103 60-61 0.7388744234975775 1103 62-63 0.7676162790101202 1103 64-65 0.6270747200817368 1103 66-67 0.6401446402890159 1103 68-69 0.653018991655749 1103 70-71 0.6765624757748085 1103 72-73 0.6919254900844152 1103 74-75 0.7109127380975337 1103 76-77 0.8831930484287867 1103 78-79 0.720305893463383 1103 80-81 0.5509014575855744 1103 82-83 0.5540485164879527 1103 84-85 0.46837718729054245 1103 86-87 0.4589717278012131 1103 88-89 0.6181632689562413 1103 90-91 0.7890176943188472 1103 92-93 0.817502826110978 1103 94-95 0.7449927139537245 1103 96-97 0.9318683925082354 1103 98-99 0.9873840945119134 1103 100-101 1.2604520762257607 1104 1 4.976161792213958 1104 2 3.6983089337023287 1104 3 1.8352835665633052 1104 4 1.9407318163126632 1104 5 1.1247981760716783 1104 6 0.826202637363906 1104 7 0.4880428875582865 1104 8 0.8224161772386722 1104 9 0.8058292723145755 1104 10-11 0.8132871600588345 1104 12-13 0.47502086068925564 1104 14-15 0.495463426431094 1104 16-17 0.6206904191087474 1104 18-19 0.4745744358476003 1104 20-21 0.5070812265411533 1104 22-23 0.593807024904919 1104 24-25 0.6935746526208106 1104 26-27 0.5309721843177968 1104 28-29 0.849309677923209 1104 30-31 0.4723976982060165 1104 32-33 0.7313777840638096 1104 34-35 0.45120775281547054 1104 36-37 0.24661766910675453 1104 38-39 0.19223118409599493 1104 40-41 0.38943635996629666 1104 42-43 0.5885930500623857 1104 44-45 0.4820576878028504 1104 46-47 0.4961702375657566 1104 48-49 0.27272122058805337 1104 50-51 0.35214392117327975 1104 52-53 0.4345390722329441 1104 54-55 0.5615935037135884 1104 56-57 0.5234240994326669 1104 58-59 0.44237694930851745 1104 60-61 0.5111889084799515 1104 62-63 0.757195719483235 1104 64-65 0.6902967847559509 1104 66-67 0.4786438324662683 1104 68-69 0.4758802323597777 1104 70-71 0.5142764120031913 1104 72-73 0.559302260610032 1104 74-75 0.5290697025191093 1104 76-77 0.47819937445800775 1104 78-79 0.42945156429295395 1104 80-81 0.41679007045087246 1104 82-83 0.40700906392026326 1104 84-85 0.38001867312870274 1104 86-87 0.30479740487887597 1104 88-89 0.5227624986635142 1104 90-91 0.5730837447528003 1104 92-93 0.605694209791956 1104 94-95 0.3922012415591283 1104 96-97 0.6812551159359295 1104 98-99 0.7702457463623027 1104 100-101 1.0322815280016648 1105 1 5.038318225837305 1105 2 3.731245027501217 1105 3 2.6171556590257694 1105 4 2.2503483095888654 1105 5 1.2549639403347896 1105 6 0.856238760375291 1105 7 0.46151181833771915 1105 8 0.8120203018763661 1105 9 0.7135703798700845 1105 10-11 0.7595165381884712 1105 12-13 0.42308152016207146 1105 14-15 0.4240644679866392 1105 16-17 0.4501792488910823 1105 18-19 0.2647402131084817 1105 20-21 0.3814957122610636 1105 22-23 0.4755647726072709 1105 24-25 0.6760293393194132 1105 26-27 0.5081212116740303 1105 28-29 0.8371640298306247 1105 30-31 0.5490771432904253 1105 32-33 0.6320123882582038 1105 34-35 0.35931426549515066 1105 36-37 0.15330565047835165 1105 38-39 0.23911580352869777 1105 40-41 0.3719038295549879 1105 42-43 0.539138372661391 1105 44-45 0.3477703114943438 1105 46-47 0.2734976745503843 1105 48-49 0.26494847917928865 1105 50-51 0.2112701699120052 1105 52-53 0.3693776819823782 1105 54-55 0.44774658711910575 1105 56-57 0.40938457471550294 1105 58-59 0.4037996466123701 1105 60-61 0.4764724316721356 1105 62-63 0.5707310752748782 1105 64-65 0.47741148164039515 1105 66-67 0.37585799679738585 1105 68-69 0.31168236332573684 1105 70-71 0.41224726944560786 1105 72-73 0.5314709660175225 1105 74-75 0.592744653340624 1105 76-77 0.6309212243982785 1105 78-79 0.6309089243503578 1105 80-81 0.4913484257907115 1105 82-83 0.47858623335779527 1105 84-85 0.268719563678431 1105 86-87 0.2635032086914819 1105 88-89 0.41742405768073 1105 90-91 0.4766720927779282 1105 92-93 0.5111095886789663 1105 94-95 0.5447825478417734 1105 96-97 0.6521919600626873 1105 98-99 0.6989098900075668 1105 100-101 0.876195469287854 1106 1 3.4831147130150946 1106 2 2.737565022613463 1106 3 0.5557993616793695 1106 4 1.039153823093855 1106 5 0.6502444702288059 1106 6 0.5337268015032635 1106 7 0.22210752661634814 1106 8 0.571218357690455 1106 9 0.5504352883784094 1106 10-11 0.614703003598521 1106 12-13 0.27496180835620976 1106 14-15 0.2588150565320788 1106 16-17 0.32086688165153276 1106 18-19 0.23783543350599246 1106 20-21 0.2897492196587379 1106 22-23 0.37720919965980926 1106 24-25 0.4128983957077281 1106 26-27 0.3145577426383568 1106 28-29 0.5928966086779042 1106 30-31 0.37509829781613035 1106 32-33 0.48115443983025585 1106 34-35 0.2027405004356666 1106 36-37 -7.943626913444746E-4 1106 38-39 0.08200959836722888 1106 40-41 0.1093817592163262 1106 42-43 0.3839369907656973 1106 44-45 0.2533299460125633 1106 46-47 0.256094372326082 1106 48-49 0.1578841482937321 1106 50-51 0.23641082359414867 1106 52-53 0.29764288734087785 1106 54-55 0.4025246802615854 1106 56-57 0.437958176877963 1106 58-59 0.3044263089137971 1106 60-61 0.5290836368968996 1106 62-63 0.6654879876884081 1106 64-65 0.5285719010656251 1106 66-67 0.4590496280460954 1106 68-69 0.3661778410522061 1106 70-71 0.3894190403958149 1106 72-73 0.37237673199336996 1106 74-75 0.479246400470835 1106 76-77 0.4707813444400628 1106 78-79 0.3248946239312147 1106 80-81 0.2482574544242837 1106 82-83 0.34163994387653673 1106 84-85 0.3476812530176687 1106 86-87 0.32131793359793903 1106 88-89 0.4340417532269818 1106 90-91 0.6206959683150224 1106 92-93 0.6375583990776938 1106 94-95 0.38569495037286217 1106 96-97 0.6470286567712584 1106 98-99 0.7775047576400418 1106 100-101 0.954339863749901 1107 1 2.6793176518088906 1107 2 2.5077451119425547 1107 3 0.8035372620778425 1107 4 1.5065646757777031 1107 5 0.7988177034614736 1107 6 0.5953503526760784 1107 7 0.20615538931054544 1107 8 0.5910249896891031 1107 9 0.3908894762992645 1107 10-11 0.479199222170692 1107 12-13 0.07796456288599529 1107 14-15 -0.021357411418584604 1107 16-17 0.007987317658852078 1107 18-19 -0.06934165511601265 1107 20-21 -8.882892386523622E-4 1107 22-23 -0.0549286247288876 1107 24-25 0.1062132795092765 1107 26-27 0.05142740635583465 1107 28-29 0.48851808435125577 1107 30-31 0.08701147462091541 1107 32-33 0.1865248708626126 1107 34-35 -0.15040577650010079 1107 36-37 -0.27136393259475966 1107 38-39 -0.27131222491757967 1107 40-41 0.005616430521499183 1107 42-43 0.21963407882925168 1107 44-45 -0.04813866402101752 1107 46-47 -0.15807003807740472 1107 48-49 -0.22420570753958202 1107 50-51 -0.13917600365635963 1107 52-53 -0.1513512865163662 1107 54-55 -0.059748469659574255 1107 56-57 0.08889196825685985 1107 58-59 0.022483071810640354 1107 60-61 0.029164903721607516 1107 62-63 0.1732988709384955 1107 64-65 0.09574434448653335 1107 66-67 0.03305152415560997 1107 68-69 0.06954269559978599 1107 70-71 0.07667099594777937 1107 72-73 0.1125415065324944 1107 74-75 0.1978748863463764 1107 76-77 0.15158323361901438 1107 78-79 0.2559802383502827 1107 80-81 0.20547374500669946 1107 82-83 0.06724683702326928 1107 84-85 -0.0014184207586538378 1107 86-87 -0.1568347802664114 1107 88-89 0.1287654054149705 1107 90-91 0.094803738255969 1107 92-93 0.1957589888991329 1107 94-95 0.1549688301324288 1107 96-97 0.27212143583481563 1107 98-99 0.3760037384786976 1107 100-101 0.5084089351924135 1108 1 3.7018138483987 1108 2 2.107333701656753 1108 3 -1.0753003265038323 1108 4 -1.3757190001514914 1108 5 -0.16338040726606096 1108 6 0.17160937056777215 1108 7 0.023881442624478666 1108 8 0.4898332423224403 1108 9 0.3946737593590086 1108 10-11 0.47821690353208623 1108 12-13 0.22817623983880253 1108 14-15 0.04620156156265409 1108 16-17 0.1349720863755124 1108 18-19 -0.03255032090251575 1108 20-21 0.06543876969683282 1108 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-0.41854482453512176 2316 96-97 -0.8172152667942818 2316 98-99 -0.8271721282857314 2316 100-101 -0.8896634668419665 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 82.0 11 713.0 12 2388.0 13 5353.0 14 9955.0 15 14893.0 16 20549.0 17 25889.0 18 33681.0 19 45523.0 20 49947.0 21 51901.0 22 54382.0 23 58909.0 24 66423.0 25 77182.0 26 91362.0 27 110887.0 28 135648.0 29 166537.0 30 206493.0 31 259321.0 32 332149.0 33 440926.0 34 639879.0 35 1007619.0 36 1737000.0 37 3030259.0 38 2666997.0 39 461576.0 40 5850.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 21.75664596188409 31.132735523208698 26.951337028785133 20.159281486122076 2 22.730230920588372 29.347968007543418 30.90130815880801 17.0204929130602 3 23.45963584322524 30.17908268271872 26.77535932156519 19.585922152490852 4 24.76971717811462 26.98503673924198 27.194244665231754 21.05100141741164 5 23.98190298118851 28.369122689841813 25.020545935088563 22.628428393881116 6 22.935890045546792 27.200457364572127 26.416724356560117 23.44692823332096 7 23.520523191970245 28.503422401836097 25.748956014818624 22.227098391375034 8 23.164003067498946 26.751786347360472 26.929673852585793 23.154536732554785 9 22.806333334236502 27.609846750354265 26.378613464617917 23.20520645079131 10-11 23.034578584025052 27.457943921277007 26.50249602623479 23.004981468463154 12-13 22.676584190729546 27.88681514813417 26.77837760397241 22.65822305716388 14-15 22.64123200926393 27.759386748941967 26.725517958941264 22.873863282852838 16-17 22.73293863984299 27.94960551889558 26.90161856280659 22.41583727845484 18-19 22.418431817791173 27.936754721927258 27.116583164504327 22.528230295777245 20-21 22.23353346700792 28.01501709570981 26.988228807242642 22.763220630039626 22-23 21.996997867873162 28.11918064891472 27.44639772509916 22.43742375811296 24-25 22.156680882821252 27.83974595676154 27.675825105821005 22.327748054596196 26-27 22.141243768313608 27.89041528996192 27.965960119577932 22.002380822146538 28-29 22.087576769224615 27.88323859492924 27.946374951551217 22.082809684294933 30-31 22.079896368086345 28.112091336842965 27.69914959122624 22.108862703844448 32-33 21.933831481738476 28.2460966807784 27.75729748407932 22.062774353403807 34-35 22.171299511874114 28.251730506144945 27.60271925974954 21.9742507222314 36-37 22.162200516338988 28.30346009608845 27.699993509882866 21.8343458776897 38-39 22.080996772894242 28.395122619096103 27.624433406408134 21.89944720160152 40-41 22.09923183398182 28.566377278763046 27.28775733159417 22.04663355566097 42-43 21.914880537891083 28.730223148120555 27.42654868575423 21.928347628234135 44-45 22.047719158254424 28.788417738565947 27.228211690726383 21.93565141245325 46-47 22.03093449011401 28.89512565697181 27.330857065829605 21.74308278708457 48-49 21.98683187832076 28.97951184751197 27.238030951804674 21.795625322362593 50-51 21.874849971715303 29.124102381037254 27.0666605251208 21.934387122126644 52-53 21.78348036493314 29.381911832182034 27.090326362481203 21.744281440403622 54-55 21.87555738033265 29.3608220065747 26.98527523141828 21.778345381674367 56-57 21.844778324422414 29.44246141820076 27.02697965628448 21.68578060109234 58-59 21.892177188408017 29.61852313187826 26.868623439641144 21.62067624007258 60-61 21.78962711366968 29.77009172460695 26.636627376886747 21.803653784836623 62-63 21.707768735557828 30.03017350506307 26.641041555355915 21.621016204023192 64-65 21.750608258187174 30.12970614183904 26.483848948913426 21.63583665106036 66-67 21.751787826963877 30.245030144806417 26.433806213380056 21.569375814849646 68-69 21.69547446501456 30.41254156990026 26.26747341372241 21.624510551362768 70-71 21.64678506327725 30.593108629048267 26.095789859789566 21.66431644788492 72-73 21.465860972293786 30.84723351720428 26.14872560638442 21.53817990411751 74-75 21.541314074619613 30.937976624249075 25.986363736045732 21.534345565085584 76-77 21.61891177282693 31.019672449569963 25.91921033493468 21.44220544266843 78-79 21.502877198520306 31.05778333828524 25.873258814593026 21.566080648601435 80-81 21.39339291282229 31.26529468308204 25.678920617623348 21.662391786472327 82-83 21.36871433877947 31.42946399291532 25.690180912837494 21.511640755467717 84-85 21.42900083681385 31.41177176852728 25.528766354511873 21.630461040146997 86-87 21.491926914209124 31.543869171049167 25.52341063637203 21.440793278369686 88-89 21.43013394434363 31.703735929548493 25.35251302341927 21.513617102688603 90-91 21.352783568100538 31.8839192426835 25.131423756693856 21.631873432522102 92-93 21.257977801019315 32.1556819351832 25.124929383506156 21.461410880291325 94-95 21.378265345771432 32.20860347597384 24.918094611360804 21.495036566893923 96-97 21.355990139248046 32.272582925915536 24.87273878198486 21.49868815285156 98-99 21.352990649908694 32.37887501027708 24.76695896338937 21.501175376424854 100-101 21.328545218493375 32.5871463926456 24.461113748986804 21.623194639874225 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 64637.0 1 40032.0 2 13027.5 3 10132.5 4 9442.5 5 9250.0 6 9273.0 7 9279.5 8 9451.5 9 9818.5 10 10174.5 11 10648.0 12 11816.0 13 13852.5 14 16151.0 15 18835.5 16 30418.5 17 35220.5 18 32771.0 19 44967.0 20 143562.5 21 167977.5 22 92094.0 23 77560.0 24 74912.5 25 78535.0 26 86056.0 27 96985.0 28 108018.5 29 118232.5 30 131842.5 31 147318.0 32 164345.0 33 186115.0 34 204112.5 35 214831.5 36 229774.0 37 251339.5 38 275166.0 39 298056.5 40 317892.5 41 333755.0 42 349611.0 43 367125.5 44 383461.0 45 400159.0 46 414213.0 47 423086.0 48 427597.5 49 430353.0 50 430170.0 51 428946.5 52 427093.5 53 417305.5 54 396952.5 55 366716.0 56 329305.0 57 292470.5 58 260755.0 59 227204.5 60 186142.5 61 147345.0 62 118320.0 63 93821.0 64 73372.0 65 56863.5 66 42724.5 67 31271.0 68 23221.0 69 18359.0 70 15226.5 71 13041.5 72 11130.0 73 9020.0 74 6934.5 75 5219.5 76 3832.5 77 2627.5 78 1636.0 79 997.0 80 552.5 81 321.0 82 202.5 83 120.5 84 53.5 85 18.5 86 7.5 87 2.5 88 2.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.1070977783494085 2 0.0020236619424462074 3 2.5401614340328965E-4 4 1.1007366214142553E-4 5 0.001278547921796558 6 8.467204780109655E-6 7 0.0 8 0.0 9 8.467204780109655E-6 10-11 4.233602390054828E-6 12-13 0.0 14-15 0.0014140231982783125 16-17 9.737285497126103E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 5.67302720267347E-4 28-29 0.0010202981760032135 30-31 9.737285497126103E-5 32-33 3.386881912043862E-5 34-35 0.0 36-37 7.197124063093207E-5 38-39 0.0 40-41 4.233602390054828E-6 42-43 8.467204780109655E-6 44-45 5.0803228680657934E-5 46-47 2.1168011950274137E-4 48-49 7.197124063093207E-5 50-51 0.0 52-53 0.0 54-55 2.116801195027414E-5 56-57 3.471553959844959E-4 58-59 0.003128632166250518 60-61 0.005321638204298918 62-63 0.0020575307615666462 64-65 0.006197993899040268 66-67 0.0016553385345114378 68-69 3.810242151049345E-4 70-71 2.1168011950274137E-4 72-73 7.408804182595948E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.233602390054828E-6 82-83 0.0 84-85 0.0 86-87 3.640898055447152E-4 88-89 9.017573090816783E-4 90-91 8.340196708408011E-4 92-93 8.467204780109655E-6 94-95 0.0 96-97 1.4817608365191897E-4 98-99 1.1854086692153517E-4 100-101 0.004026155872942141 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.1810273E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.533550273160415 #Duplication Level Percentage of deduplicated Percentage of total 1 76.97547879998969 41.97746142939832 2 12.175030966068414 13.278953265307534 3 4.282598214785486 7.006358551372541 4 2.0836683219483563 4.5451932475025 5 1.1358692398216585 3.097149114677546 6 0.6902991907605616 2.2586679373717824 7 0.48245248004419916 1.841689260243088 8 0.3456993908571165 1.5081772088565994 9 0.2606728404004663 1.2793873902143715 >10 1.4783564013651507 14.099926388522425 >50 0.06920723192431033 2.58572101563448 >100 0.01900195647412055 1.4885041838053952 >500 7.462336482511204E-4 0.2899443157235124 >1k 6.494502991161961E-4 0.6993152234228817 >5k 3.1680189758732356E-5 0.09433741202566603 >10k+ 2.376014231904927E-4 3.94921405592144 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 85020 0.7198817504049229 No Hit ATATGTATACATATGTATACATATGTATACATATGTATACATATGTATAC 41890 0.35469120823879347 No Hit ATACATATGTATACATATGTATACATATGTATACATATGTATACATATGT 39822 0.33718102875352673 No Hit ACATATGTATACATATGTATACATATGTATACATATGTATACATATGTAT 34393 0.2912125740023114 No Hit GTATACATATGTATACATATGTATACATATGTATACATATGTATACATAT 32335 0.27378706656484575 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31740 0.26874907972068046 No Hit ATGTATACATATGTATACATATGTATACATATGTATACATATGTATACAT 30759 0.26044275183139287 No Hit TATACATATGTATACATATGTATACATATGTATACATATGTATACATATG 28388 0.2403670092977529 No Hit CATATGTATACATATGTATACATATGTATACATATGTATACATATGTATA 27663 0.23422828583217337 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 23858 0.20201057164385616 No Hit TATGTATACATATGTATACATATGTATACATATGTATACATATGTATACA 21506 0.18209570600103825 No Hit TACATATGTATACATATGTATACATATGTATACATATGTATACATATGTA 19872 0.16826029339033907 No Hit TGTATACATATGTATACATATGTATACATATGTATACATATGTATACATA 17657 0.1495054348023962 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11996 0.10157258854219542 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.65696262906031E-4 0.0 0.0 8.467204780109655E-6 0.0 2 4.65696262906031E-4 0.0 0.0 8.467204780109655E-6 0.0 3 4.65696262906031E-4 0.0 0.0 1.693440956021931E-5 0.0 4 4.741634676861407E-4 0.0 0.0 2.5401614340328967E-5 0.0 5 4.741634676861407E-4 0.0 0.0 2.5401614340328967E-5 0.0 6 4.741634676861407E-4 8.467204780109655E-6 0.0 2.5401614340328967E-5 0.0 7 4.995650820264697E-4 8.467204780109655E-6 0.0 2.5401614340328967E-5 8.467204780109655E-6 8 5.249666963667986E-4 8.467204780109655E-6 0.0 4.233602390054828E-5 8.467204780109655E-6 9 5.757699250474566E-4 8.467204780109655E-6 0.0 5.9270433460767585E-5 8.467204780109655E-6 10-11 6.604419728485531E-4 8.467204780109655E-6 0.0 6.773763824087724E-5 8.467204780109655E-6 12-13 0.0012489127050661743 8.467204780109655E-6 0.0 6.773763824087724E-5 1.2700807170164482E-5 14-15 0.0026587023009544317 1.693440956021931E-5 0.0 7.62048430209869E-5 1.693440956021931E-5 16-17 0.005317404601908863 1.693440956021931E-5 0.0 7.62048430209869E-5 1.693440956021931E-5 18-19 0.010842255720930415 1.693440956021931E-5 0.0 8.467204780109656E-5 1.693440956021931E-5 20-21 0.021561736972549237 1.693440956021931E-5 0.0 9.313925258120621E-5 1.693440956021931E-5 22-23 0.040824627847298706 1.693440956021931E-5 0.0 9.313925258120621E-5 1.693440956021931E-5 24-25 0.07067999190196535 1.693440956021931E-5 0.0 9.737285497126104E-5 1.693440956021931E-5 26-27 0.10559874441513756 1.693440956021931E-5 0.0 1.0160645736131587E-4 1.693440956021931E-5 28-29 0.14015764072515513 2.116801195027414E-5 0.0 1.0160645736131587E-4 2.116801195027414E-5 30-31 0.17387828376194184 2.5401614340328967E-5 0.0 1.0160645736131587E-4 2.9635216730383792E-5 32-33 0.20955061750054382 2.5401614340328967E-5 0.0 1.1007366214142551E-4 4.233602390054828E-5 34-35 0.24946925443637075 2.5401614340328967E-5 0.0 1.1854086692153517E-4 4.233602390054828E-5 36-37 0.28595443983386326 2.5401614340328967E-5 0.0 1.1854086692153517E-4 4.233602390054828E-5 38-39 0.3136040970433114 2.5401614340328967E-5 0.0 1.1854086692153517E-4 4.233602390054828E-5 40-41 0.3361141609512329 2.5401614340328967E-5 0.0 1.1854086692153517E-4 4.233602390054828E-5 42-43 0.36109241505255635 2.5401614340328967E-5 0.0 1.2277446931159E-4 4.233602390054828E-5 44-45 0.39137113934622847 2.5401614340328967E-5 0.0 1.3124167409169965E-4 4.233602390054828E-5 46-47 0.4199183202623682 2.9635216730383792E-5 0.0 1.3547527648175448E-4 4.233602390054828E-5 48-49 0.4415731964874986 3.386881912043862E-5 0.0 1.4394248126186414E-4 4.233602390054828E-5 50-51 0.4593077568994383 3.386881912043862E-5 0.0 1.4394248126186414E-4 5.0803228680657934E-5 52-53 0.5059451208282824 3.386881912043862E-5 0.0 1.4394248126186414E-4 5.0803228680657934E-5 54-55 0.5735938534189684 4.233602390054828E-5 0.0 1.4394248126186414E-4 5.0803228680657934E-5 56-57 0.6384865108537288 4.233602390054828E-5 0.0 1.524096860419738E-4 5.0803228680657934E-5 58-59 0.6925707813866793 5.0803228680657934E-5 0.0 1.6087689082208345E-4 5.0803228680657934E-5 60-61 0.7392420141346436 5.0803228680657934E-5 0.0 1.6511049321213828E-4 5.0803228680657934E-5 62-63 0.787445810947808 5.0803228680657934E-5 0.0 1.7357769799224794E-4 5.0803228680657934E-5 64-65 0.8443538942749249 5.0803228680657934E-5 0.0 1.7781130038230277E-4 5.0803228680657934E-5 66-67 0.9041238928177189 5.0803228680657934E-5 0.0 1.8627850516241242E-4 5.0803228680657934E-5 68-69 0.9584537122892925 5.0803228680657934E-5 0.0 1.8627850516241242E-4 5.5036831070712756E-5 70-71 1.0094643874870632 5.0803228680657934E-5 0.0 1.9474570994252208E-4 5.9270433460767585E-5 72-73 1.066431741247641 5.0803228680657934E-5 0.0 1.989793123325769E-4 5.9270433460767585E-5 74-75 1.1334369662750388 5.0803228680657934E-5 0.0 2.0321291472263174E-4 5.9270433460767585E-5 76-77 1.20689420134488 5.0803228680657934E-5 0.0 2.0744651711268657E-4 5.9270433460767585E-5 78-79 1.280160924307169 5.0803228680657934E-5 0.0 2.116801195027414E-4 5.9270433460767585E-5 80-81 1.3569754060723236 5.0803228680657934E-5 0.0 2.2014732428285105E-4 5.9270433460767585E-5 82-83 1.446422957369402 5.0803228680657934E-5 0.0 2.2014732428285105E-4 5.9270433460767585E-5 84-85 1.557051221423925 5.0803228680657934E-5 0.0 2.2014732428285105E-4 5.9270433460767585E-5 86-87 1.6840423587160092 5.0803228680657934E-5 0.0 2.2014732428285105E-4 6.773763824087724E-5 88-89 1.823306709336863 5.0803228680657934E-5 0.0 2.3284813145301554E-4 6.773763824087724E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 3850 0.0 78.75287 1 TCGACGG 4615 0.0 77.383766 2 ACGGCGC 6260 0.0 58.640526 5 GACGGCG 6250 0.0 57.898792 4 CGACGGC 6435 0.0 55.79147 3 CGGCGCG 8305 0.0 44.02913 6 ATCTCGA 9415 0.0 38.799107 1 TCTCGAG 11915 0.0 32.443977 2 CTCGAGG 13020 0.0 30.054586 3 TCGAGGG 13190 0.0 29.88325 4 GGGCGCG 13515 0.0 29.726469 8 TTCGACG 1055 0.0 28.969112 1 CGAGGGC 14200 0.0 27.991734 5 AGGGCGC 14495 0.0 27.74944 7 GAGGGCG 15285 0.0 26.004534 6 TATCTCG 11905 0.0 24.852058 2 CGCGCCG 20495 0.0 24.376099 9 GGCGCGC 21500 0.0 23.17 7 GCGCGCC 22120 0.0 22.584976 8 ATATCTC 13930 0.0 20.407677 1 >>END_MODULE