FastQCFastQC Report
Tue 31 May 2016
SRR1051275_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051275_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8044059
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT4218935.244777543277592No Hit
ATATGTATACATATGTATACATATGTATACATATGTATACATATGTATAC613350.76248819159581No Hit
ATACATATGTATACATATGTATACATATGTATACATATGTATACATATGT549050.6825534223456093No Hit
ACATATGTATACATATGTATACATATGTATACATATGTATACATATGTAT542670.6746221030949674No Hit
TATACATATGTATACATATGTATACATATGTATACATATGTATACATATG502800.6250575735458926No Hit
GTATACATATGTATACATATGTATACATATGTATACATATGTATACATAT500300.6219496898269891No Hit
CATATGTATACATATGTATACATATGTATACATATGTATACATATGTATA478890.5953337736582986No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT475140.5906719480799432No Hit
ATGTATACATATGTATACATATGTATACATATGTATACATATGTATACAT474670.5900876659407893No Hit
TATGTATACATATGTATACATATGTATACATATGTATACATATGTATACA454590.5651251439105556No Hit
TACATATGTATACATATGTATACATATGTATACATATGTATACATATGTA439530.5464032523878803No Hit
ATATATGTACATATATGTACATATATGTACATATATGTACATATATGTAC422150.5247972447740624No Hit
TGTATACATATGTATACATATGTATACATATGTATACATATGTATACATA394000.48980247409920785No Hit
ACATATATGTACATATATGTACATATATGTACATATATGTACATATATGT326940.40643660122333763No Hit
ATATGTACATATATGTACATATATGTACATATATGTACATATATGTACAT317330.39448989620787217No Hit
ATGTACATATATGTACATATATGTACATATATGTACATATATGTACATAT298930.3716158720367417No Hit
CATATATGTACATATATGTACATATATGTACATATATGTACATATATGTA298370.3709197060837072No Hit
TATATGTACATATATGTACATATATGTACATATATGTACATATATGTACA286940.35671046172088No Hit
TATGTACATATATGTACATATATGTACATATATGTACATATATGTACATA275110.34200395596302813No Hit
GTACATATATGTACATATATGTACATATATGTACATATATGTACATATAT272980.33935603903452227No Hit
TACATATATGTACATATATGTACATATATGTACATATATGTACATATATG257460.32006229690756866No Hit
TGTACATATATGTACATATATGTACATATATGTACATATATGTACATATA239880.29820765859623855No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT111780.1389596968396179No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG512050.091.092451
TCGACGG527300.090.110142
ATATCTC746500.089.322921
TATCTCG749050.089.270352
ACGGCGC531500.089.0000845
GACGGCG531750.088.931044
CGGCGCG536050.088.5447166
CGACGGC538150.088.191343
ATCTCGA767650.086.670433
TCTCGAG780850.084.8148964
CTCGAGG792150.083.3809055
TCGAGGG797000.082.899896
CGAGGGC804950.082.439097
AGGGCGC812100.082.030719
GAGGGCG812550.081.747718
TCGCGAG10100.074.694774
TATCGCG10650.072.1339952
ATCGCGA10550.068.457823
AATTCGA20150.059.4721
ATATCGC13300.058.913051
Produced by FastQC (version 0.11.5)