##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051275_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8044059 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.036099436863903 31.0 25.0 33.0 10.0 34.0 2 29.083369229390286 31.0 28.0 34.0 16.0 34.0 3 30.502476548220244 31.0 30.0 34.0 25.0 34.0 4 34.34459866592227 37.0 35.0 37.0 28.0 37.0 5 34.86716295840197 37.0 35.0 37.0 32.0 37.0 6 35.210783138214175 37.0 35.0 37.0 33.0 37.0 7 35.50957271695794 37.0 35.0 37.0 33.0 37.0 8 35.34483909180676 37.0 35.0 37.0 33.0 37.0 9 37.32255593848827 39.0 37.0 39.0 34.0 39.0 10-11 37.3905447486151 39.0 37.0 39.0 34.5 39.0 12-13 37.56269888124888 39.0 37.0 39.0 35.0 39.0 14-15 38.74397396886323 40.0 38.0 41.0 35.5 41.0 16-17 38.7369533216999 40.0 38.0 41.0 35.5 41.0 18-19 38.81711602314205 40.0 38.5 41.0 35.0 41.0 20-21 38.80138596696021 40.0 39.0 41.0 35.0 41.0 22-23 38.6616608605183 40.0 38.0 41.0 34.5 41.0 24-25 38.522950478110616 40.0 38.0 41.0 34.0 41.0 26-27 38.328485221204865 40.0 38.0 41.0 34.0 41.0 28-29 38.05480721859449 40.0 38.0 41.0 33.5 41.0 30-31 38.087270928768675 40.0 38.0 41.0 34.0 41.0 32-33 37.83958440633019 40.0 37.5 41.0 33.0 41.0 34-35 38.1202323354416 40.0 38.0 41.0 34.0 41.0 36-37 38.06351681408602 40.0 38.0 41.0 33.5 41.0 38-39 37.9094463379744 40.0 37.5 41.0 33.0 41.0 40-41 37.67812350705036 40.0 37.0 41.0 33.0 41.0 42-43 37.45511208955578 39.5 37.0 41.0 32.0 41.0 44-45 37.40449168759204 39.5 37.0 41.0 32.0 41.0 46-47 37.42999162487496 40.0 37.0 41.0 32.0 41.0 48-49 37.52494946891862 40.0 37.0 41.0 32.5 41.0 50-51 37.36840418500162 39.5 36.5 41.0 32.0 41.0 52-53 37.14656754755279 39.0 36.0 41.0 31.0 41.0 54-55 36.91849786283268 39.0 35.5 40.0 31.0 41.0 56-57 36.5612733571447 39.0 35.0 40.0 30.0 41.0 58-59 36.07717484419246 38.5 35.0 40.0 30.0 41.0 60-61 35.74047940225202 38.0 35.0 40.0 29.5 41.0 62-63 35.31747088130507 38.0 34.0 40.0 28.0 41.0 64-65 35.08542217305965 37.0 34.0 40.0 28.0 41.0 66-67 34.86322060790454 37.0 34.0 39.5 28.0 41.0 68-69 34.43731404754739 36.5 34.0 39.0 27.5 40.5 70-71 33.97302617994224 36.0 33.0 39.0 26.0 40.0 72-73 33.36227537615028 35.0 33.0 38.5 26.0 40.0 74-75 32.97729007208923 35.0 32.5 37.5 25.5 39.0 76-77 32.06783496988274 34.0 31.0 36.0 24.0 39.0 78-79 32.467252975146 35.0 32.0 37.0 25.5 39.0 80-81 32.3807053130764 35.0 32.5 36.0 25.0 39.0 82-83 32.314665891933416 35.0 33.0 36.0 25.0 39.0 84-85 32.171118647936325 35.0 33.0 36.0 25.0 38.5 86-87 31.933663962434885 35.0 33.0 35.5 24.5 38.0 88-89 31.515284820760264 35.0 32.0 35.0 23.0 37.5 90-91 31.007235153297607 34.0 31.5 35.0 20.0 37.0 92-93 30.50310272463193 34.0 31.0 35.0 18.0 37.0 94-95 30.494339860013458 34.0 31.0 35.0 15.0 37.0 96-97 30.12458759936992 34.0 31.0 35.0 5.0 36.0 98-99 29.838511751343447 34.0 31.0 35.0 2.0 36.0 100-101 29.212382393018252 33.5 30.0 35.0 2.0 35.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.078458218952381 1101 2 3.0111795681660958 1101 3 1.67933740030114 1101 4 1.3625792670374892 1101 5 0.712085023226436 1101 6 0.5373343936554704 1101 7 0.2613867000369865 1101 8 0.7175864697436012 1101 9 0.7158096047858393 1101 10-11 0.8039336905671135 1101 12-13 0.45399121404156517 1101 14-15 0.3270487917553382 1101 16-17 0.3625635790367525 1101 18-19 0.34294654359743504 1101 20-21 0.48968152608322413 1101 22-23 0.5434662989770871 1101 24-25 0.7379835677489837 1101 26-27 0.4919243995284077 1101 28-29 0.8274111949586853 1101 30-31 0.5751106085856961 1101 32-33 0.5538027150687697 1101 34-35 0.32288723067310343 1101 36-37 0.32511621926446566 1101 38-39 0.3446129694793427 1101 40-41 0.4315928948363492 1101 42-43 0.5531137327145004 1101 44-45 0.22750216448304883 1101 46-47 0.1569783409460328 1101 48-49 0.2484412627132997 1101 50-51 0.1725503998571014 1101 52-53 0.06859853467027932 1101 54-55 0.15948157144718067 1101 56-57 0.1598402416170046 1101 58-59 -0.15867482571887592 1101 60-61 0.16746022661293836 1101 62-63 0.005868867258584487 1101 64-65 -0.10419276622372564 1101 66-67 0.16757818341854147 1101 68-69 0.058718243811284765 1101 70-71 0.08823243090930788 1101 72-73 -0.30506354989734774 1101 74-75 -0.29553757218342724 1101 76-77 -0.14636709078698473 1101 78-79 -0.4112668749790558 1101 80-81 -0.6232720205279207 1101 82-83 -0.21947256389314163 1101 84-85 -0.2139961789301843 1101 86-87 -0.2988949298375516 1101 88-89 -0.23868428022025512 1101 90-91 0.08258451891918028 1101 92-93 -0.2957888640482942 1101 94-95 -0.5643469153656504 1101 96-97 -0.08852173156713405 1101 98-99 0.2265981256192866 1101 100-101 0.36302714842345907 1102 1 4.21341404555864 1102 2 3.069012975850317 1102 3 1.8273146665433941 1102 4 1.496313347914814 1102 5 0.786456512709016 1102 6 0.5711242296917476 1102 7 0.33019388230063385 1102 8 0.6915327020430979 1102 9 0.8055268931444104 1102 10-11 0.9027625119811873 1102 12-13 0.5427252612928584 1102 14-15 0.42271636611129537 1102 16-17 0.6790939588731817 1102 18-19 0.5681677628388258 1102 20-21 0.513952313614439 1102 22-23 0.6488075215106264 1102 24-25 0.5157292851539097 1102 26-27 0.4769619373857097 1102 28-29 0.8272358592348112 1102 30-31 0.501165128868756 1102 32-33 0.7536720699389861 1102 34-35 0.417330097007067 1102 36-37 0.23116573407289565 1102 38-39 0.03008910284466282 1102 40-41 0.36657896885652264 1102 42-43 0.47273511323273 1102 44-45 0.5042676030737638 1102 46-47 0.4390859147767827 1102 48-49 0.2521010556858485 1102 50-51 0.3001783941349103 1102 52-53 0.1894209408784846 1102 54-55 0.46206131237303083 1102 56-57 0.23922064840228785 1102 58-59 -0.12458940087321224 1102 60-61 -0.24313544728631342 1102 62-63 0.40818885511477987 1102 64-65 0.4436959034376855 1102 66-67 0.025134640721006463 1102 68-69 -0.10645281179944277 1102 70-71 0.16399988097705176 1102 72-73 -0.17062180122854187 1102 74-75 -0.182236347971795 1102 76-77 -0.5012890287607306 1102 78-79 -0.10122351661620854 1102 80-81 -0.16325056712126695 1102 82-83 -0.22481205708803742 1102 84-85 -0.432475379309615 1102 86-87 -0.6491725527398913 1102 88-89 0.019251702865531684 1102 90-91 -0.36635355506512823 1102 92-93 -0.6132499643368057 1102 94-95 -0.638990455437149 1102 96-97 -0.298347833756317 1102 98-99 0.01958604170537015 1102 100-101 0.46307026980285926 1103 1 4.338071265491596 1103 2 3.2139521680906533 1103 3 1.9690124151837622 1103 4 1.644242592285714 1103 5 0.9796811314572196 1103 6 0.7312468939039221 1103 7 0.4137450522080499 1103 8 0.8191619343434979 1103 9 0.7908454319814382 1103 10-11 0.8395296746819128 1103 12-13 0.5049435605646408 1103 14-15 0.4380127866544754 1103 16-17 0.3821643784994109 1103 18-19 0.4005498929218305 1103 20-21 0.5185065044591823 1103 22-23 0.5872736508515999 1103 24-25 0.7313351009713926 1103 26-27 0.5081251157611746 1103 28-29 0.8724874608639439 1103 30-31 0.581791070463666 1103 32-33 0.6110771414063478 1103 34-35 0.34545605874755836 1103 36-37 0.3458506852631942 1103 38-39 0.4054495927647608 1103 40-41 0.5725208695321413 1103 42-43 0.5518789022742538 1103 44-45 0.28602340521610614 1103 46-47 0.19955349785593057 1103 48-49 0.24514559688581983 1103 50-51 0.11218709662895066 1103 52-53 0.03158061555290459 1103 54-55 0.1425719904106515 1103 56-57 0.2876826891046562 1103 58-59 -0.016443547942401437 1103 60-61 0.17970345296816248 1103 62-63 -0.014305492683483578 1103 64-65 -0.2620402316285606 1103 66-67 0.09115486709995935 1103 68-69 0.005788544676185836 1103 70-71 -0.07743608192106421 1103 72-73 -0.06354619760613645 1103 74-75 0.08227367226727011 1103 76-77 0.23341564571672535 1103 78-79 0.022070978206826908 1103 80-81 -0.2118071446056149 1103 82-83 0.13428946106823503 1103 84-85 -0.14192239192063028 1103 86-87 -0.29659037333603067 1103 88-89 -0.15931662615571085 1103 90-91 0.24114344198115134 1103 92-93 -0.09835882075985936 1103 94-95 -0.0911014825561196 1103 96-97 0.20835593876106628 1103 98-99 0.48048689777846576 1103 100-101 0.7497836304894321 1104 1 4.265647691079536 1104 2 3.0755756699934835 1104 3 1.8405742555867057 1104 4 1.6232881720064611 1104 5 0.9577919992908903 1104 6 0.6790131873044487 1104 7 0.4422808459276979 1104 8 0.7453256939593871 1104 9 0.8288752456579473 1104 10-11 0.8806675824649872 1104 12-13 0.5610245264017237 1104 14-15 0.5358545872935636 1104 16-17 0.6874913565363912 1104 18-19 0.561990618059113 1104 20-21 0.5459465588137888 1104 22-23 0.6768887898232947 1104 24-25 0.6252339337243598 1104 26-27 0.4174791050127453 1104 28-29 0.797736894783938 1104 30-31 0.4592894166046122 1104 32-33 0.6633474561153108 1104 34-35 0.4556298982604403 1104 36-37 0.2394481103553474 1104 38-39 0.027757038862262107 1104 40-41 0.2752562317581422 1104 42-43 0.4311481891442668 1104 44-45 0.5092854794048165 1104 46-47 0.5382101443983132 1104 48-49 0.21546302802595108 1104 50-51 0.3382090393208941 1104 52-53 0.2568790325695076 1104 54-55 0.5257485301666236 1104 56-57 0.3481001425979642 1104 58-59 0.017157795851048263 1104 60-61 0.10359345933722608 1104 62-63 0.5931517412382945 1104 64-65 0.6975333152283341 1104 66-67 0.38603567958467977 1104 68-69 0.2717687677810616 1104 70-71 0.32795838679444245 1104 72-73 0.08983611721702545 1104 74-75 -0.08758592050218539 1104 76-77 -0.3464415909580225 1104 78-79 -0.11839340731133063 1104 80-81 -0.164478623404257 1104 82-83 -0.0631454044149038 1104 84-85 0.12774215487673501 1104 86-87 -0.1625541187746471 1104 88-89 0.36691717492321274 1104 90-91 0.28122897373590305 1104 92-93 0.014956661366664292 1104 94-95 -0.3694449698864055 1104 96-97 0.18598192990822326 1104 98-99 0.47692560759514535 1104 100-101 0.8431108170346384 1105 1 4.252783775792558 1105 2 3.115495871931852 1105 3 1.8714313064366337 1105 4 1.585851411498986 1105 5 0.9108939635939777 1105 6 0.664430615086971 1105 7 0.2971140016885627 1105 8 0.749035582835198 1105 9 0.7055730499246806 1105 10-11 0.7816672874450177 1105 12-13 0.4207204036122647 1105 14-15 0.32150627316200797 1105 16-17 0.31766544124839413 1105 18-19 0.33840597509697545 1105 20-21 0.43051754933188136 1105 22-23 0.5021599381439543 1105 24-25 0.7755279983199728 1105 26-27 0.5459451820470989 1105 28-29 0.9086638052420639 1105 30-31 0.6834922639804475 1105 32-33 0.6042587391770411 1105 34-35 0.35155450817423173 1105 36-37 0.359893134479222 1105 38-39 0.33038944244957946 1105 40-41 0.5910928936199014 1105 42-43 0.7065316259383962 1105 44-45 0.3856517428775206 1105 46-47 0.20970358491714336 1105 48-49 0.2711998926311452 1105 50-51 0.12342968521591757 1105 52-53 0.08959993195750116 1105 54-55 0.21003917528205562 1105 56-57 0.29642266872301803 1105 58-59 -0.05081576088220885 1105 60-61 -0.06704588817438406 1105 62-63 -0.044448732424164916 1105 64-65 -0.16211763385447853 1105 66-67 0.03567401261484093 1105 68-69 -0.11400557557021784 1105 70-71 -0.1468590090912798 1105 72-73 0.1377922886628582 1105 74-75 0.28689573093706855 1105 76-77 0.3270730038684775 1105 78-79 0.2553304657149269 1105 80-81 0.07600159420736219 1105 82-83 0.23878446694313737 1105 84-85 -0.2268036111790579 1105 86-87 -0.4159896874829627 1105 88-89 -0.06645796564437845 1105 90-91 -0.04520510866282379 1105 92-93 -0.4577588700845503 1105 94-95 0.07554028930570666 1105 96-97 0.1360280427016427 1105 98-99 0.3306863734038039 1105 100-101 0.6729579977086395 1106 1 4.26967304670961 1106 2 3.1060911649212706 1106 3 1.8731711420333497 1106 4 1.6650163020250162 1106 5 1.0010817321016887 1106 6 0.7346302331340766 1106 7 0.46897717603936684 1106 8 0.726473387268932 1106 9 0.8809815058217936 1106 10-11 0.8793830213979419 1106 12-13 0.5453150080800668 1106 14-15 0.4825807781691509 1106 16-17 0.6717434803117115 1106 18-19 0.5979652365837467 1106 20-21 0.5388903448409508 1106 22-23 0.6365797655345276 1106 24-25 0.5172057774579173 1106 26-27 0.5048819919227086 1106 28-29 0.8153119218233371 1106 30-31 0.5495974766649141 1106 32-33 0.6521809449429128 1106 34-35 0.4706721481175862 1106 36-37 0.24586641761450778 1106 38-39 0.13585583480433172 1106 40-41 0.2611021158420712 1106 42-43 0.5021649327727644 1106 44-45 0.43831780456769565 1106 46-47 0.5082407183930755 1106 48-49 0.2784868271228902 1106 50-51 0.33505735420490623 1106 52-53 0.2858963823696783 1106 54-55 0.4626614846406696 1106 56-57 0.483911933253232 1106 58-59 0.07077975855001739 1106 60-61 0.3630911097261773 1106 62-63 0.5773232599870965 1106 64-65 0.5772556179271859 1106 66-67 0.5852303201841806 1106 68-69 0.3121061473450695 1106 70-71 0.3463428438689462 1106 72-73 0.10730049406971176 1106 74-75 0.2114970609498883 1106 76-77 0.22445833937398874 1106 78-79 0.12046786521278108 1106 80-81 -0.07276517550369022 1106 82-83 0.2715779462611181 1106 84-85 0.3897068881708847 1106 86-87 0.18555961922947262 1106 88-89 0.31150489096071254 1106 90-91 0.5111833970420392 1106 92-93 0.39229448671238387 1106 94-95 -0.15915373710391023 1106 96-97 0.4468531461129466 1106 98-99 0.7649717019902269 1106 100-101 0.9580549641007501 1107 1 4.217474305817394 1107 2 3.0879145940072306 1107 3 1.8990770714355776 1107 4 1.6210438458425074 1107 5 0.9462400124219172 1107 6 0.7374496501637893 1107 7 0.3244704514574579 1107 8 0.7727742325972784 1107 9 0.6795088223729664 1107 10-11 0.8061996995048943 1107 12-13 0.4458561043578939 1107 14-15 0.2703924846638657 1107 16-17 0.3652955979184753 1107 18-19 0.29343806001048023 1107 20-21 0.4084418717763114 1107 22-23 0.39490177898455414 1107 24-25 0.7120500149251257 1107 26-27 0.45052648954966656 1107 28-29 0.9440696718841792 1107 30-31 0.6951900188952109 1107 32-33 0.6966245584753565 1107 34-35 0.3177384441064959 1107 36-37 0.3968852734249495 1107 38-39 0.2799630429894293 1107 40-41 0.41775736261350715 1107 42-43 0.717440162586314 1107 44-45 0.4645090645994401 1107 46-47 0.2325474839771573 1107 48-49 0.28502954943396475 1107 50-51 0.13112469749194844 1107 52-53 0.060063587700767584 1107 54-55 0.19893852511589927 1107 56-57 0.2942917149989128 1107 58-59 -0.0703174476598889 1107 60-61 -0.20229744262654492 1107 62-63 -0.09377988923426273 1107 64-65 -0.14868750590363788 1107 66-67 -0.14792805860455616 1107 68-69 -0.16481514656360474 1107 70-71 -0.13799968728014989 1107 72-73 0.09822918232990929 1107 74-75 0.05575381119013656 1107 76-77 -0.15193022179716564 1107 78-79 0.17662650801901947 1107 80-81 0.035562169717827885 1107 82-83 0.07333586494228683 1107 84-85 -0.30262117895130913 1107 86-87 -0.615095461550883 1107 88-89 -0.01093672951530067 1107 90-91 -0.2898532957137405 1107 92-93 -0.618915426143225 1107 94-95 -0.13265380903571256 1107 96-97 0.05350203617493676 1107 98-99 0.273774616099427 1107 100-101 0.6351552859359266 1108 1 4.263822144352186 1108 2 3.143666840298682 1108 3 1.9092896684167684 1108 4 1.778601928172968 1108 5 1.0900100183711672 1108 6 0.8019514625813216 1108 7 0.49139786231783233 1108 8 0.7395697601171349 1108 9 0.8500938985652766 1108 10-11 0.8165167900280963 1108 12-13 0.4871805248630139 1108 14-15 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0.11039357789894666 2316 80-81 -0.11781452264728642 2316 82-83 0.021445819166309832 2316 84-85 -0.15501824704558942 2316 86-87 -0.309225935397901 2316 88-89 -0.4517286177431252 2316 90-91 -0.209848834792826 2316 92-93 0.06769276851236228 2316 94-95 0.0658300839949284 2316 96-97 -0.17935388459378743 2316 98-99 -0.11790534893397364 2316 100-101 -0.220503603569302 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 118.0 11 1338.0 12 4351.0 13 8412.0 14 13320.0 15 18128.0 16 22410.0 17 27828.0 18 35410.0 19 44651.0 20 50155.0 21 51730.0 22 53073.0 23 55507.0 24 60586.0 25 66996.0 26 75697.0 27 87623.0 28 103752.0 29 123221.0 30 150656.0 31 187026.0 32 236524.0 33 312809.0 34 455881.0 35 707958.0 36 1248639.0 37 2071252.0 38 1482544.0 39 285310.0 40 1153.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 19.191061421243766 35.26641129548443 25.804880488463468 19.737646794808338 2 19.54729714357678 30.452932849196472 32.40333372660025 17.59643628062649 3 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20.43070089933542 30.59628786540513 29.1874641272165 19.78554710804295 48-49 20.356432416284857 30.68931885613086 29.04018057074363 19.914068156840656 50-51 19.970757300512094 31.029546153254223 28.446273206101548 20.55342334013214 52-53 19.808208766245997 31.308820584234898 28.79272143578261 20.090249213736495 54-55 20.12528127901598 31.125237892959262 28.779873444488658 19.969607383536097 56-57 20.270049776861594 30.929599253406124 28.881426320504662 19.91892464922762 58-59 20.40824191887166 31.099430791630972 28.425707364276636 20.06661992522073 60-61 20.066115632608543 31.439948945572656 27.890059915349415 20.603875506469393 62-63 20.051356930668142 31.69998153835826 28.162563147206416 20.08609838376718 64-65 20.237140852222062 31.589351227665862 28.107638776645928 20.06586914346615 66-67 20.3615524283655 31.511527222569974 28.11874012047811 20.00818022858642 68-69 20.4023306798044 31.702932805238188 27.688477319444733 20.206259195512683 70-71 20.102342757175414 31.980983453914213 27.36626057963979 20.550413209270584 72-73 19.894444847439 32.24549044427384 27.726918621350848 20.133146086936307 74-75 20.172677759615993 32.14675775915851 27.57727333783065 20.10329114339484 76-77 20.33774242580767 31.865119338383767 27.702811478632867 20.094326757175697 78-79 20.338233471435256 31.669906946231997 27.5995365026537 20.392323079679052 80-81 19.902470879440344 32.00427172401396 27.18761759455021 20.905639801995484 82-83 19.929702156585375 32.00391120950257 27.52763250493314 20.538754128978916 84-85 20.148988216023774 31.750115209249458 27.442383254523616 20.658513320203145 86-87 20.330145634715983 31.780742542682923 27.477719250340204 20.411392572260887 88-89 20.239173025657685 32.04403745410123 27.11722031736868 20.599569202872402 90-91 20.09348690297595 32.25264586893869 26.727507744215114 20.926359483870247 92-93 20.014559813646322 32.58407851061261 26.840976676078583 20.560384999662485 94-95 20.237830180012352 32.53823100999135 26.78992657811187 20.434012231884424 96-97 20.23065147040825 32.512071202019534 26.761516583423894 20.495760744148324 98-99 20.44422879798877 32.47701509833907 26.57187214792385 20.506883955748304 100-101 20.029852995257983 32.906835331365514 26.127879281111227 20.935432392265273 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 37046.0 1 22573.0 2 6809.0 3 5117.0 4 4584.5 5 4354.0 6 4241.0 7 4171.0 8 4155.0 9 4261.0 10 4418.0 11 4555.0 12 5062.0 13 6190.0 14 7427.5 15 9046.0 16 15027.5 17 20006.5 18 26524.0 19 65059.0 20 447030.5 21 502141.0 22 164726.5 23 106798.0 24 81853.5 25 70595.5 26 70046.0 27 77518.0 28 82381.0 29 81343.0 30 88209.5 31 98701.5 32 109051.0 33 121617.0 34 129354.5 35 131094.0 36 133982.5 37 146925.0 38 169431.5 39 187841.0 40 198909.5 41 204633.5 42 210420.5 43 220154.5 44 229960.5 45 239902.5 46 248991.0 47 254948.5 48 256705.0 49 258699.0 50 255956.0 51 249527.5 52 246736.0 53 242318.0 54 227965.0 55 205696.0 56 183783.5 57 163501.0 58 142259.0 59 119611.5 60 96783.5 61 76156.0 62 60310.5 63 47313.0 64 37041.5 65 28451.5 66 21145.5 67 15582.5 68 11733.5 69 9317.0 70 7822.0 71 6779.0 72 5884.0 73 4741.0 74 3550.0 75 2696.5 76 2032.0 77 1397.0 78 826.5 79 485.5 80 254.0 81 148.5 82 104.5 83 57.0 84 16.0 85 5.5 86 1.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.054458078937512 2 0.004077543439201527 3 3.729460462684324E-5 4 1.4917841850737296E-4 5 0.0 6 1.2431534875614411E-5 7 0.0 8 0.0 9 0.0 10-11 6.215767437807206E-6 12-13 0.0 14-15 0.0 16-17 1.2431534875614411E-5 18-19 0.0 20-21 1.2431534875614411E-5 22-23 0.0 24-25 0.0 26-27 0.0013115269293773207 28-29 0.0018585144639043545 30-31 3.1078837189036035E-5 32-33 0.0 34-35 0.0 36-37 1.2431534875614414E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 9.945227900491529E-5 48-49 1.1809958131833693E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 7.52107859974672E-4 58-59 0.0064581823678816885 60-61 0.010914887620789454 62-63 0.004400763345967502 64-65 0.012319651061733883 66-67 0.003406240555918349 68-69 6.713028832831783E-4 70-71 1.4296265106956575E-4 72-73 0.0013985476735066216 74-75 6.215767437807206E-6 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 7.894024646015153E-4 88-89 0.0016596099058945241 90-91 0.0018833775336555836 92-93 0.0 94-95 0.0 96-97 2.1755186032325225E-4 98-99 3.542987439550108E-4 100-101 0.008018339994771298 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8044059.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.86788358250916 #Duplication Level Percentage of deduplicated Percentage of total 1 80.65876867545119 41.83599623566838 2 11.118223710295815 11.533574660998328 3 3.4109614840275375 5.307580594738888 4 1.5937075338604632 3.306489473233691 5 0.8347789835468059 2.164910956786552 6 0.5381087856203489 1.674633830836898 7 0.37139628325631197 1.3484477428040489 8 0.2677496207506946 1.1110084926686403 9 0.18978895003119875 0.8859556048918378 >10 0.9407127025614241 8.310469320648597 >50 0.04789013569081607 1.6976961115795692 >100 0.023889841725103438 2.150796439999167 >500 0.0013927032845225168 0.5169296737359138 >1k 0.0019600473756137752 1.985809373864992 >5k 1.0316038803108934E-4 0.3597015467547045 >10k+ 5.673821341709914E-4 15.809999940789831 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATGTATACATATGTATACATATGTATACATATGTATACATATGTATAC 92554 1.1505882788776165 No Hit ATACATATGTATACATATGTATACATATGTATACATATGTATACATATGT 87044 1.082090521712981 No Hit ACATATGTATACATATGTATACATATGTATACATATGTATACATATGTAT 85233 1.0595770120532433 No Hit ATGTATACATATGTATACATATGTATACATATGTATACATATGTATACAT 80837 1.0049279847400423 No Hit CATATGTATACATATGTATACATATGTATACATATGTATACATATGTATA 71432 0.8880093992348888 No Hit GTATACATATGTATACATATGTATACATATGTATACATATGTATACATAT 67253 0.836058014989696 No Hit TATACATATGTATACATATGTATACATATGTATACATATGTATACATATG 61263 0.7615931210847658 No Hit ATATATGTACATATATGTACATATATGTACATATATGTACATATATGTAC 60670 0.7542212209035264 No Hit TATGTATACATATGTATACATATGTATACATATGTATACATATGTATACA 55829 0.6940401605706771 No Hit TACATATGTATACATATGTATACATATGTATACATATGTATACATATGTA 53668 0.6671756137044743 No Hit TGTATACATATGTATACATATGTATACATATGTATACATATGTATACATA 50034 0.6219994159664916 No Hit ACATATATGTACATATATGTACATATATGTACATATATGTACATATATGT 49824 0.6193887936426126 No Hit ATGTACATATATGTACATATATGTACATATATGTACATATATGTACATAT 49545 0.6159203954123161 No Hit ATATGTACATATATGTACATATATGTACATATATGTACATATATGTACAT 49170 0.6112585698339607 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 48067 0.597546586866158 No Hit CATATATGTACATATATGTACATATATGTACATATATGTACATATATGTA 46571 0.5789490106922388 No Hit GTACATATATGTACATATATGTACATATATGTACATATATGTACATATAT 35797 0.44501165394236913 No Hit TATGTACATATATGTACATATATGTACATATATGTACATATATGTACATA 33718 0.41916649293596675 No Hit TATATGTACATATATGTACATATATGTACATATATGTACATATATGTACA 32789 0.40761759703652095 No Hit TACATATATGTACATATATGTACATATATGTACATATATGTACATATATG 30707 0.38173514142549175 No Hit TGTACATATATGTACATATATGTACATATATGTACATATATGTACATATA 27937 0.34729978982003984 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11957 0.14864386250772155 No Hit TTATACATATGTATACATATGTATACATATGTATACATATGTATACATAT 8791 0.1092856230915263 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 6.713028832831783E-4 0.0 0.0 0.0 0.0 2 6.713028832831783E-4 0.0 0.0 0.0 0.0 3 6.837344181587927E-4 0.0 0.0 0.0 0.0 4 6.961659530344071E-4 0.0 0.0 0.0 0.0 5 7.085974879100216E-4 0.0 0.0 0.0 0.0 6 7.085974879100216E-4 0.0 0.0 0.0 0.0 7 7.334605576612504E-4 0.0 0.0 0.0 0.0 8 7.707551622880936E-4 0.0 0.0 0.0 0.0 9 8.702074412930089E-4 0.0 0.0 0.0 0.0 10-11 0.0011312696736809117 0.0 0.0 6.2157674378072065E-6 0.0 12-13 0.00291519492833158 0.0 0.0 1.2431534875614413E-5 0.0 14-15 0.008086713436587175 1.2431534875614413E-5 0.0 1.2431534875614413E-5 0.0 16-17 0.01795735212782502 1.2431534875614413E-5 0.0 1.864730231342162E-5 0.0 18-19 0.03840101123077292 1.2431534875614413E-5 0.0 2.4863069751228826E-5 6.2157674378072065E-6 20-21 0.07266232134796624 1.2431534875614413E-5 0.0 2.4863069751228826E-5 1.2431534875614413E-5 22-23 0.13199182154183603 1.2431534875614413E-5 0.0 3.1078837189036035E-5 1.2431534875614413E-5 24-25 0.22410949497013882 1.864730231342162E-5 0.0 3.729460462684324E-5 1.2431534875614413E-5 26-27 0.3416993833585755 2.4863069751228826E-5 0.0 4.3510372064650446E-5 1.2431534875614413E-5 28-29 0.4673200432766592 2.4863069751228826E-5 0.0 4.972613950245765E-5 1.2431534875614413E-5 30-31 0.5883099564535765 2.4863069751228826E-5 0.0 6.215767437807207E-5 1.2431534875614413E-5 32-33 0.705296915400546 2.4863069751228826E-5 6.2157674378072065E-6 7.458920925368648E-5 1.2431534875614413E-5 34-35 0.820294828767417 2.4863069751228826E-5 1.2431534875614413E-5 8.080497669149368E-5 1.2431534875614413E-5 36-37 0.9287288917199639 2.4863069751228826E-5 1.2431534875614413E-5 8.702074412930089E-5 1.2431534875614413E-5 38-39 1.018770498824039 2.4863069751228826E-5 1.2431534875614413E-5 8.702074412930089E-5 1.2431534875614413E-5 40-41 1.0930799985430242 2.4863069751228826E-5 1.2431534875614413E-5 8.702074412930089E-5 1.2431534875614413E-5 42-43 1.163374112497186 4.972613950245765E-5 1.2431534875614413E-5 8.702074412930089E-5 1.2431534875614413E-5 44-45 1.2398342677496523 4.972613950245765E-5 1.2431534875614413E-5 9.32365115671081E-5 1.2431534875614413E-5 46-47 1.3174940661176155 4.972613950245765E-5 1.2431534875614413E-5 9.94522790049153E-5 1.2431534875614413E-5 48-49 1.3853267361664057 4.972613950245765E-5 1.2431534875614413E-5 9.94522790049153E-5 1.2431534875614413E-5 50-51 1.4435684770586592 4.972613950245765E-5 1.2431534875614413E-5 9.94522790049153E-5 1.2431534875614413E-5 52-53 1.6133645961572385 4.972613950245765E-5 1.2431534875614413E-5 9.94522790049153E-5 1.2431534875614413E-5 54-55 1.8282623237845472 4.972613950245765E-5 1.2431534875614413E-5 1.056680464427225E-4 1.2431534875614413E-5 56-57 2.041960408296359 5.594190694026486E-5 1.2431534875614413E-5 1.1188381388052972E-4 1.2431534875614413E-5 58-59 2.243432078258004 6.215767437807207E-5 1.2431534875614413E-5 1.1188381388052972E-4 1.2431534875614413E-5 60-61 2.4231050518152593 6.215767437807207E-5 1.2431534875614413E-5 1.1188381388052972E-4 1.2431534875614413E-5 62-63 2.59581636584217 6.215767437807207E-5 1.2431534875614413E-5 1.1188381388052972E-4 1.2431534875614413E-5 64-65 2.7754706920971115 6.215767437807207E-5 1.2431534875614413E-5 1.1188381388052972E-4 1.2431534875614413E-5 66-67 2.9654431922988134 6.215767437807207E-5 1.2431534875614413E-5 1.1188381388052972E-4 1.2431534875614413E-5 68-69 3.1505114519920854 6.215767437807207E-5 1.2431534875614413E-5 1.1188381388052972E-4 1.2431534875614413E-5 70-71 3.326846558435238 6.215767437807207E-5 1.2431534875614413E-5 1.1188381388052972E-4 1.2431534875614413E-5 72-73 3.5068551834341344 6.215767437807207E-5 1.2431534875614413E-5 1.1188381388052972E-4 1.2431534875614413E-5 74-75 3.6994693847969042 6.215767437807207E-5 1.2431534875614413E-5 1.1188381388052972E-4 1.2431534875614413E-5 76-77 3.909711502613295 6.215767437807207E-5 1.2431534875614413E-5 1.1188381388052972E-4 1.2431534875614413E-5 78-79 4.126318566286001 6.215767437807207E-5 1.2431534875614413E-5 1.1188381388052972E-4 1.864730231342162E-5 80-81 4.334677306568736 6.837344181587928E-5 1.2431534875614413E-5 1.1188381388052972E-4 2.4863069751228826E-5 82-83 4.5535096149841765 7.458920925368648E-5 1.2431534875614413E-5 1.1188381388052972E-4 2.4863069751228826E-5 84-85 4.798429499336094 7.458920925368648E-5 1.2431534875614413E-5 1.1188381388052972E-4 2.4863069751228826E-5 86-87 5.068995640136404 7.458920925368648E-5 1.2431534875614413E-5 1.1188381388052972E-4 2.4863069751228826E-5 88-89 5.355977622739962 7.458920925368648E-5 1.2431534875614413E-5 1.1188381388052972E-4 2.4863069751228826E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGACGG 3275 0.0 82.63421 2 GTCGACG 2470 0.0 81.75127 1 GACGGCG 4045 0.0 67.25622 4 ACGGCGC 4080 0.0 66.67885 5 CGACGGC 4590 0.0 59.373528 3 AATTCGA 2160 0.0 56.136276 1 CGGCGCG 4940 0.0 54.87857 6 GCGGTAC 1475 0.0 51.501705 9 GGCGGTA 1520 0.0 48.72756 8 ATTCGAG 1625 0.0 43.82624 2 TTCGACG 965 0.0 42.055016 1 CGAGGCG 1950 0.0 37.252075 5 TATCTCG 2500 0.0 37.223087 2 CGCGCCG 9450 0.0 35.671406 9 TATCGGA 1670 0.0 34.081024 1 GGCGCGC 9900 0.0 33.954056 7 AGGCGGT 2255 0.0 33.687366 7 TACGGCG 795 0.0 33.445168 88-89 GCGCGCC 10070 0.0 33.428 8 ATCTCGA 3955 0.0 33.28637 1 >>END_MODULE