FastQCFastQC Report
Tue 31 May 2016
SRR1051274_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051274_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27354912
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT24999569.138965608809125No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT2259250.8259028579583806No Hit
ATATGTATACATATGTATACATATGTATACATATGTATACATATGTATAC1231950.4503578735694709No Hit
ATACATATGTATACATATGTATACATATGTATACATATGTATACATATGT1138600.4162323753774093No Hit
ACATATGTATACATATGTATACATATGTATACATATGTATACATATGTAT1035780.37864497608327163No Hit
GTATACATATGTATACATATGTATACATATGTATACATATGTATACATAT952900.34834694405158384No Hit
ATGTATACATATGTATACATATGTATACATATGTATACATATGTATACAT848690.31025140932641276No Hit
TATACATATGTATACATATGTATACATATGTATACATATGTATACATATG788980.2884235196954755No Hit
CATATGTATACATATGTATACATATGTATACATATGTATACATATGTATA765170.27971941565741465No Hit
TATGTATACATATGTATACATATGTATACATATGTATACATATGTATACA642330.23481340389616318No Hit
ATATATGTACATATATGTACATATATGTACATATATGTACATATATGTAC626310.22895705166223893No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT600490.2195181618570003No Hit
TACATATGTATACATATGTATACATATGTATACATATGTATACATATGTA577330.21105167510683276No Hit
TGTATACATATGTATACATATGTATACATATGTATACATATGTATACATA463720.16951982883366615No Hit
ACATATATGTACATATATGTACATATATGTACATATATGTACATATATGT419890.1534971123284915No Hit
ATATGTACATATATGTACATATATGTACATATATGTACATATATGTACAT415040.15172412179574915No Hit
CATATATGTACATATATGTACATATATGTACATATATGTACATATATGTA353310.1291577907470512No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT342540.1252206550691883No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319070.11664084315094854No Hit
TATATGTACATATATGTACATATATGTACATATATGTACATATATGTACA317240.1159718590942643No Hit
ATGTACATATATGTACATATATGTACATATATGTACATATATGTACATAT315310.11526631853175034No Hit
GTACATATATGTACATATATGTACATATATGTACATATATGTACATATAT301050.11005336080042954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG2150400.093.168131
TCGACGG2192800.091.845282
ATATCTC3371000.091.683541
TATCTCG3383650.091.672042
GACGGCG2218350.090.558114
ACGGCGC2213150.090.5550465
CGACGGC2226000.090.1890263
CGGCGCG2237800.089.9757166
ATCTCGA3452600.089.107383
TCTCGAG3502850.087.384454
CTCGAGG3551400.086.113855
TCGAGGG3577400.085.5073246
CGAGGGC3587550.085.2221157
AGGGCGC3621000.084.716439
GAGGGCG3634300.084.4475258
TCGCGAG20400.062.74814
TATCGCG21350.061.728242
ATCGCGA24450.052.162523
CGGTGCG27950.049.325316
CCATATT2299250.044.0011518-19
Produced by FastQC (version 0.11.5)