FastQCFastQC Report
Tue 31 May 2016
SRR1051274_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051274_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27354912
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1934680.7072514069867964No Hit
ATATGTATACATATGTATACATATGTATACATATGTATACATATGTATAC1447060.5289945732598226No Hit
ATACATATGTATACATATGTATACATATGTATACATATGTATACATATGT1407890.5146753899263138No Hit
ACATATGTATACATATGTATACATATGTATACATATGTATACATATGTAT1312090.4796542573414237No Hit
GTATACATATGTATACATATGTATACATATGTATACATATGTATACATAT1117390.4084787404909217No Hit
CATATGTATACATATGTATACATATGTATACATATGTATACATATGTATA1032510.37744957834263915No Hit
ATGTATACATATGTATACATATGTATACATATGTATACATATGTATACAT980530.35844750661234076No Hit
TATACATATGTATACATATGTATACATATGTATACATATGTATACATATG917860.3355375444088433No Hit
TACATATGTATACATATGTATACATATGTATACATATGTATACATATGTA718250.2625671031221011No Hit
ATATATGTACATATATGTACATATATGTACATATATGTACATATATGTAC715780.2616641574281065No Hit
TATGTATACATATGTATACATATGTATACATATGTATACATATGTATACA692940.2533146514965941No Hit
TGTATACATATGTATACATATGTATACATATGTATACATATGTATACATA523200.19126363850119496No Hit
ACATATATGTACATATATGTACATATATGTACATATATGTACATATATGT504920.18458110923551865No Hit
CATATATGTACATATATGTACATATATGTACATATATGTACATATATGTA492460.18002616860913315No Hit
ATATGTACATATATGTACATATATGTACATATATGTACATATATGTACAT485970.1776536513807831No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT470030.17182654435152267No Hit
ATGTACATATATGTACATATATGTACATATATGTACATATATGTACATAT353820.12934422892678288No Hit
GTACATATATGTACATATATGTACATATATGTACATATATGTACATATAT343260.12548386191116243No Hit
TATATGTACATATATGTACATATATGTACATATATGTACATATATGTACA330330.12075710570737716No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT306520.1120530016693163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG82000.075.589061
TCGACGG89950.075.2897342
ACGGCGC130000.063.6005635
GACGGCG122450.058.059194
CGACGGC133800.051.9628453
CGGCGCG167250.049.150486
TATCTCG81400.041.8323442
AATTCGA58300.033.9556271
ATCTCGA152300.033.1262661
TACGGCG33800.032.456614
CGCGCCG348450.030.4470989
GGGCGCG206400.029.1977628
CTACGGC36050.028.7183283
TCTCGAG189900.028.3099382
GGCGCGC375300.028.2810677
TCGAGGG203600.027.6873194
GCGCGCC388800.027.3968498
CTCGAGG201300.027.0599883
CGAGGGC224500.026.9711485
GCGGTAC39800.026.4894589
Produced by FastQC (version 0.11.5)