##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051274_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 27354912 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.07367488515408 31.0 28.0 33.0 16.0 34.0 2 28.803332651920066 31.0 28.0 34.0 16.0 34.0 3 28.399197299556292 31.0 27.0 34.0 16.0 34.0 4 32.281254533006724 35.0 32.0 37.0 19.0 37.0 5 34.05281822877003 35.0 33.0 37.0 28.0 37.0 6 34.820801434126345 36.0 35.0 37.0 32.0 37.0 7 35.270538102992255 37.0 35.0 37.0 33.0 37.0 8 35.33920306524839 37.0 35.0 37.0 33.0 37.0 9 37.179619916159844 39.0 37.0 39.0 34.0 39.0 10-11 37.23688202689155 39.0 37.0 39.0 34.0 39.0 12-13 37.323103086568146 39.0 37.0 39.0 34.0 39.0 14-15 38.57147409211187 40.0 38.0 41.0 34.0 41.0 16-17 38.608812505044796 40.0 38.0 41.0 34.0 41.0 18-19 38.54847078287074 40.0 38.0 41.0 34.0 41.0 20-21 38.46685706757163 40.0 38.0 41.0 34.0 41.0 22-23 38.352550942222 40.0 38.0 41.0 34.0 41.0 24-25 38.32662303940148 40.0 38.0 41.0 34.0 41.0 26-27 38.09914155454055 40.0 38.0 41.0 33.5 41.0 28-29 37.91211980502807 40.0 37.0 41.0 33.0 41.0 30-31 37.743281005619764 40.0 37.0 41.0 33.0 41.0 32-33 37.57501956138627 40.0 37.0 41.0 32.0 41.0 34-35 37.53538115933256 40.0 37.0 41.0 32.0 41.0 36-37 37.36629132639871 39.5 36.5 41.0 32.0 41.0 38-39 37.069835647798826 39.0 36.0 40.5 31.0 41.0 40-41 36.861716261415864 39.0 35.5 40.0 30.0 41.0 42-43 36.670436903617166 39.0 35.0 40.0 30.0 41.0 44-45 36.784647726156095 39.0 35.0 40.0 30.0 41.0 46-47 36.834341360703334 39.0 35.0 40.0 30.0 41.0 48-49 36.87219474513389 39.0 35.0 40.0 30.0 41.0 50-51 36.94766667500155 39.0 35.0 40.5 31.0 41.0 52-53 36.753641960171535 39.0 35.0 40.0 30.0 41.0 54-55 36.60193017619651 39.0 35.0 40.0 30.0 41.0 56-57 36.34977549918639 39.0 35.0 40.0 30.0 41.0 58-59 36.03467112231982 38.0 35.0 40.0 29.5 41.0 60-61 35.71784060208273 38.0 34.5 40.0 29.0 41.0 62-63 35.41121362408331 37.5 34.0 40.0 28.0 41.0 64-65 35.122434903098934 37.0 34.0 40.0 28.0 41.0 66-67 34.822311711329945 36.5 34.0 39.0 28.0 41.0 68-69 34.401479138371926 36.0 33.5 39.0 27.5 40.5 70-71 33.949049223773784 35.5 33.0 39.0 26.5 40.0 72-73 33.53728173207064 35.0 33.0 38.0 26.0 40.0 74-75 33.222464104435794 35.0 33.0 37.0 26.0 39.0 76-77 32.418126221718424 35.0 32.0 36.0 26.0 39.0 78-79 32.48017248236806 35.0 32.0 36.0 26.0 39.0 80-81 32.207114795324514 35.0 32.0 36.0 25.5 38.0 82-83 32.05996115067013 35.0 32.0 35.5 25.0 37.0 84-85 31.890163474113898 35.0 32.0 35.0 25.0 37.0 86-87 31.689954294131894 35.0 32.0 35.0 25.0 36.5 88-89 31.369119429080964 34.5 32.0 35.0 24.0 36.0 90-91 31.062754415002324 34.0 32.0 35.0 22.5 36.0 92-93 30.74174875795616 34.0 31.0 35.0 20.0 36.0 94-95 30.670718772555364 34.0 32.0 35.0 20.0 35.5 96-97 30.398065089735987 34.0 31.5 35.0 16.5 35.0 98-99 30.10627402127998 34.0 31.0 35.0 4.5 35.0 100-101 29.320664402064246 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 174.0 11 2045.0 12 7387.0 13 18107.0 14 35470.0 15 56927.0 16 79050.0 17 100871.0 18 123033.0 19 145103.0 20 154170.0 21 158711.0 22 167821.0 23 184327.0 24 209200.0 25 243856.0 26 291288.0 27 350835.0 28 427490.0 29 520182.0 30 636160.0 31 786810.0 32 990656.0 33 1284878.0 34 1775114.0 35 2644071.0 36 4129539.0 37 6192406.0 38 4810754.0 39 808008.0 40 20469.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.548173845230693 31.825297396208196 25.624888320495977 19.00164043806513 2 20.718677444516707 29.522633883562182 31.827355480161085 17.931333191760025 3 22.973755333285833 30.02211709929496 27.36805056126604 19.63607700615317 4 21.745129706633605 27.003945820196833 30.3208994380468 20.93002503512276 5 23.04485532169381 29.710316562770604 25.68033217395812 21.564495941577462 6 22.1863919723083 27.508664623011764 27.550353662259997 22.754589742419938 7 22.85857837890321 29.454702687400346 26.496005543720997 21.190713389975446 8 22.06565680050442 27.40194887119359 28.29540815192533 22.23698617637666 9 22.065692121293477 28.904168535161272 26.85364809658113 22.17649124696412 10-11 21.977222736247352 28.502665517817462 27.242679650563645 22.277432095371545 12-13 21.628593095612832 28.9043706100091 27.72002936291715 21.74700693146092 14-15 21.791123864125623 28.57584618979828 27.675735687975973 21.957294258100124 16-17 22.17967306474132 28.601280370431674 27.787473584253437 21.43157298057357 18-19 21.813502414650983 28.578420271187394 27.910671165314717 21.697406148846905 20-21 21.606472247063405 28.774161722973908 27.403004632919547 22.216361397043137 22-23 21.127224890589037 29.091299089160493 28.07365240773571 21.707823612514755 24-25 21.533502136162895 28.636870294783208 28.220958221902908 21.608669347150993 26-27 21.76403755910423 28.414641493607796 28.51030464000716 21.311016307280813 28-29 21.799182247049963 28.56216339562379 28.28956323746613 21.349091119860116 30-31 21.5507398708454 28.878953535403163 27.860585591316152 21.709721002435284 32-33 21.335752679642148 29.105747984300507 28.14673615265373 21.41176318340361 34-35 21.66352483543062 28.94411896734542 28.032063330163933 21.360292867060025 36-37 21.942897576564956 28.72575311521328 28.142960209017577 21.188389099204187 38-39 21.70127116444343 28.912373943395558 28.033554991834958 21.352799900326062 40-41 21.611649180021146 29.222897764933 27.449334022511923 21.716119032533932 42-43 21.310585248636098 29.431862199683007 27.802346267515706 21.45520628416519 44-45 21.6742512297818 29.309768896208737 27.581894644638936 21.434085229370524 46-47 21.89901566162468 29.19394605035157 27.691886130985317 21.215152157038432 48-49 21.69632825138971 29.32379710229755 27.655445951625673 21.324428694687064 50-51 21.48609538248014 29.564935023985868 27.191569683301054 21.75739991023294 52-53 21.270129364178235 29.862902291404286 27.49702142100175 21.369946923415732 54-55 21.54537783328031 29.73579440531131 27.359930168292646 21.358897593115735 56-57 21.69420869011783 29.600361763803257 27.466616998475406 21.238812547603505 58-59 21.651834613536863 29.744294340229487 27.275378828361664 21.328492217871982 60-61 21.507220497222235 29.98934730330161 26.79189320258002 21.71153899689613 62-63 21.368035648408185 30.286518705088795 26.99927286456723 21.346172781935792 64-65 21.49115588472446 30.238607080907485 26.90062092545999 21.369616108908062 66-67 21.71194652522969 30.091700656308724 26.890753921717973 21.305598896743618 68-69 21.65388270761745 30.266087327682317 26.638568423724724 21.441461540975506 70-71 21.4584336886715 30.520564136250496 26.332774030345806 21.688228144732197 72-73 21.24634168328959 30.726844442696223 26.567734343018635 21.459079530995552 74-75 21.483370545724487 30.68980135431986 26.384852314364093 21.44197578559156 76-77 21.589661849396556 30.59414704020982 26.457493264829367 21.35869784556426 78-79 21.502328356969308 30.688130161047493 26.26762060137499 21.541920880608206 80-81 21.20094527819686 30.946493700765238 25.98212898525347 21.870432035784436 82-83 21.221024216784173 31.104773797115488 26.099254130300253 21.574947855800083 84-85 21.35771081990686 31.00100449966719 25.963671533653628 21.677613146772323 86-87 21.50222129595999 30.969274221775073 26.06840000980447 21.460104472460465 88-89 21.401558268308307 31.142247727486545 25.909456401024634 21.546737603180517 90-91 21.381251259382687 31.342808802828582 25.490561892267298 21.785378045521437 92-93 21.208895027157734 31.663054738704595 25.564191385545413 21.56385884859226 94-95 21.376198935683387 31.705321524558155 25.38976789754691 21.528711642211544 96-97 21.44615395694833 31.62841964418444 25.388074801805434 21.537351597061797 98-99 21.499433689211937 31.72876629760117 25.226080578217015 21.54571943496988 100-101 21.28554691894075 31.9831470062115 24.877574367404957 21.853731707442797 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 151977.0 1 94707.5 2 30926.5 3 22352.0 4 19251.5 5 18012.0 6 17604.0 7 17322.0 8 17312.5 9 17559.0 10 18150.0 11 19378.0 12 21408.0 13 25037.0 14 29610.5 15 35418.5 16 55398.0 17 67367.0 18 72220.5 19 122834.0 20 526791.5 21 625728.0 22 308227.5 23 232190.5 24 203437.0 25 196288.0 26 203486.5 27 226239.0 28 248056.5 29 262324.5 30 293842.5 31 336401.0 32 364734.0 33 398903.5 34 442224.5 35 470294.5 36 501451.0 37 555003.0 38 625712.0 39 697377.5 40 757301.5 41 802933.0 42 845210.5 43 890655.0 44 932146.0 45 970261.0 46 1002680.0 47 1022612.5 48 1025819.5 49 1020834.0 50 1001289.0 51 974089.5 52 954689.5 53 928561.5 54 870744.0 55 786511.0 56 696640.5 57 611454.5 58 536367.5 59 459352.0 60 372530.0 61 294146.5 62 234433.0 63 182549.0 64 142278.0 65 112497.0 66 85335.5 67 62624.0 68 47909.5 69 39355.5 70 34329.5 71 30753.5 72 26833.0 73 21634.5 74 16896.5 75 13969.5 76 10945.0 77 7388.0 78 4495.0 79 2805.0 80 1626.5 81 1111.0 82 831.0 83 483.0 84 234.5 85 123.5 86 50.5 87 10.0 88 3.5 89 1.5 90 1.5 91 1.5 92 0.5 93 1.0 94 0.5 95 0.5 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.1655712875259845 2 0.0015170949919341725 3 2.1568338439546068E-4 4 5.227580333652691E-4 5 0.0029647326227918405 6 0.0 7 0.0 8 0.0 9 1.096695174892173E-5 10-11 7.31130116594782E-6 12-13 1.827825291486955E-6 14-15 5.830762679843387E-4 16-17 5.849040932758256E-5 18-19 9.870256574029556E-5 20-21 8.407996340839992E-5 22-23 0.0017437453280785549 24-25 0.006927457854735559 26-27 0.004087017351764831 28-29 0.002056303452922824 30-31 0.0014915054378533553 32-33 5.117910816163474E-4 34-35 4.076050400015909E-4 36-37 3.0524682367832147E-4 38-39 0.006302341605047021 40-41 0.002226291205031111 42-43 0.0011551855842197555 44-45 0.012478563264981441 46-47 0.015373838526696777 48-49 0.0066807014403848205 50-51 0.0028495796294281625 52-53 2.0106078206356505E-5 54-55 1.096695174892173E-5 56-57 1.7181557739977377E-4 58-59 0.0017272949004551723 60-61 0.0029446265445854844 62-63 0.0011350795060133991 64-65 0.0033047081270084146 66-67 9.32190898658347E-4 68-69 2.1202773381248674E-4 70-71 1.133251680721912E-4 72-73 4.3136676879092136E-4 74-75 1.827825291486955E-6 76-77 0.0 78-79 0.0 80-81 7.31130116594782E-6 82-83 0.0 84-85 0.0 86-87 1.736434026912607E-4 88-89 4.5147284699727783E-4 90-91 0.019020349983213253 92-93 0.005706470560022272 94-95 0.016099485167417098 96-97 1.370868968615216E-4 98-99 0.007305817690073358 100-101 0.0035057689090719794 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.7354912E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.42391662500792 #Duplication Level Percentage of deduplicated Percentage of total 1 72.08452883133234 33.46446156418752 2 14.427705550147959 13.395811991004658 3 5.253556372899157 7.316719891207484 4 2.5735948623745597 4.779054132697012 5 1.4974038360285995 3.475767541887937 6 0.9396388329702725 2.617302890365901 7 0.6191165708057325 2.01192712449723 8 0.4402721513044311 1.6351326115575826 9 0.3276404771869927 1.3689318776316084 >10 1.6837128589016919 14.02664344129318 >50 0.1012426485998665 3.1940520542462956 >100 0.04862168990496409 4.053299708891761 >500 0.0015231819285260128 0.4598811244596726 >1k 0.001120382762034267 1.0991453089385297 >5k 9.31389836689735E-5 0.28261249310766057 >10k+ 2.2861386899594602E-4 6.819256244026064 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 193468 0.7072514069867964 No Hit ATATGTATACATATGTATACATATGTATACATATGTATACATATGTATAC 144706 0.5289945732598226 No Hit ATACATATGTATACATATGTATACATATGTATACATATGTATACATATGT 140789 0.5146753899263138 No Hit ACATATGTATACATATGTATACATATGTATACATATGTATACATATGTAT 131209 0.4796542573414237 No Hit GTATACATATGTATACATATGTATACATATGTATACATATGTATACATAT 111739 0.4084787404909217 No Hit CATATGTATACATATGTATACATATGTATACATATGTATACATATGTATA 103251 0.37744957834263915 No Hit ATGTATACATATGTATACATATGTATACATATGTATACATATGTATACAT 98053 0.35844750661234076 No Hit TATACATATGTATACATATGTATACATATGTATACATATGTATACATATG 91786 0.3355375444088433 No Hit TACATATGTATACATATGTATACATATGTATACATATGTATACATATGTA 71825 0.2625671031221011 No Hit ATATATGTACATATATGTACATATATGTACATATATGTACATATATGTAC 71578 0.2616641574281065 No Hit TATGTATACATATGTATACATATGTATACATATGTATACATATGTATACA 69294 0.2533146514965941 No Hit TGTATACATATGTATACATATGTATACATATGTATACATATGTATACATA 52320 0.19126363850119496 No Hit ACATATATGTACATATATGTACATATATGTACATATATGTACATATATGT 50492 0.18458110923551865 No Hit CATATATGTACATATATGTACATATATGTACATATATGTACATATATGTA 49246 0.18002616860913315 No Hit ATATGTACATATATGTACATATATGTACATATATGTACATATATGTACAT 48597 0.1776536513807831 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 47003 0.17182654435152267 No Hit ATGTACATATATGTACATATATGTACATATATGTACATATATGTACATAT 35382 0.12934422892678288 No Hit GTACATATATGTACATATATGTACATATATGTACATATATGTACATATAT 34326 0.12548386191116243 No Hit TATATGTACATATATGTACATATATGTACATATATGTACATATATGTACA 33033 0.12075710570737716 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 30652 0.1120530016693163 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.9481026296118223E-4 0.0 0.0 1.0966951748921729E-5 0.0 2 3.9846591354415615E-4 0.0 0.0 1.0966951748921729E-5 0.0 3 4.240554676249735E-4 0.0 0.0 1.0966951748921729E-5 0.0 4 4.240554676249735E-4 0.0 0.0 1.0966951748921729E-5 3.6556505829739097E-6 5 4.240554676249735E-4 0.0 0.0 1.0966951748921729E-5 3.6556505829739097E-6 6 4.240554676249735E-4 0.0 0.0 2.1933903497843457E-5 3.6556505829739097E-6 7 4.2771111820794744E-4 0.0 0.0 2.1933903497843457E-5 3.6556505829739097E-6 8 4.350224193738953E-4 0.0 0.0 2.1933903497843457E-5 3.6556505829739097E-6 9 5.227580333652691E-4 0.0 0.0 2.1933903497843457E-5 3.6556505829739097E-6 10-11 7.329579418862689E-4 0.0 0.0 2.1933903497843457E-5 3.6556505829739097E-6 12-13 0.0016870827440424593 0.0 0.0 2.741737937230432E-5 7.311301165947819E-6 14-15 0.0039554139307777705 0.0 0.0 2.9245204663791277E-5 7.311301165947819E-6 16-17 0.008029636505502192 0.0 0.0 2.9245204663791277E-5 7.311301165947819E-6 18-19 0.01681964833226296 0.0 0.0 2.9245204663791277E-5 7.311301165947819E-6 20-21 0.033465653261834656 7.311301165947819E-6 0.0 3.2900855246765186E-5 7.311301165947819E-6 22-23 0.06353886278266951 1.0966951748921729E-5 0.0 4.021215641271301E-5 7.311301165947819E-6 24-25 0.10951598016473238 1.0966951748921729E-5 0.0 4.021215641271301E-5 7.311301165947819E-6 26-27 0.16372562265965251 1.0966951748921729E-5 0.0 4.5695632287173874E-5 7.311301165947819E-6 28-29 0.21908862291350087 1.2794777040408685E-5 0.0 5.66625840360956E-5 7.311301165947819E-6 30-31 0.27397638859156265 1.4622602331895639E-5 0.0 6.397388520204341E-5 1.2794777040408685E-5 32-33 0.33524509236220534 1.4622602331895639E-5 0.0 6.580171049353037E-5 1.4622602331895639E-5 34-35 0.40473352646866495 1.6450427623382593E-5 0.0 6.762953578501733E-5 1.4622602331895639E-5 36-37 0.4705059917575315 2.558955408081737E-5 0.0 7.128518636799125E-5 1.4622602331895639E-5 38-39 0.5241380414603418 3.65565058297391E-5 0.0 7.31130116594782E-5 2.0106078206356505E-5 40-41 0.5703710543832128 3.65565058297391E-5 0.0 7.859648753393906E-5 2.558955408081737E-5 42-43 0.6181723414061796 3.65565058297391E-5 0.0 9.139126457434775E-5 2.9245204663791277E-5 44-45 0.6747746072076561 3.65565058297391E-5 0.0 9.870256574029556E-5 2.9245204663791277E-5 46-47 0.7308705654033908 4.021215641271301E-5 0.0 1.0053039103178252E-4 2.9245204663791277E-5 48-49 0.7761238639700249 4.3867806995686915E-5 0.0 1.0235821632326948E-4 2.9245204663791277E-5 50-51 0.8125816672340236 4.7523457578660826E-5 0.0 1.0235821632326948E-4 2.9245204663791277E-5 52-53 0.9052834825423675 5.30069334531217E-5 0.0 1.0784169219773034E-4 3.2900855246765186E-5 54-55 1.036289204659112 5.66625840360956E-5 0.0 1.1515299336367816E-4 4.3867806995686915E-5 56-57 1.16525872940114 5.8490409327582555E-5 0.0 1.1698081865516511E-4 4.3867806995686915E-5 58-59 1.2794064188545005 6.214605991055647E-5 0.0 1.1880864394665207E-4 5.117910816163474E-5 60-61 1.3795694170026942 6.397388520204341E-5 0.0 1.2429211982111293E-4 5.117910816163474E-5 62-63 1.4789190328961759 6.580171049353037E-5 0.0 1.334312462785477E-4 5.117910816163474E-5 64-65 1.5892812961708667 6.580171049353037E-5 0.0 1.3708689686152162E-4 5.117910816163474E-5 66-67 1.7085012008081035 6.580171049353037E-5 0.0 1.3891472215300858E-4 5.117910816163474E-5 68-69 1.822848123218236 6.945736107650429E-5 0.0 1.4257037273598247E-4 5.30069334531217E-5 70-71 1.9521923521450186 6.945736107650429E-5 3.6556505829739097E-6 1.4805384861044335E-4 5.483475874460865E-5 72-73 2.0853768420092154 6.945736107650429E-5 3.6556505829739097E-6 1.498816739019303E-4 5.8490409327582555E-5 74-75 2.210337945887013 7.128518636799125E-5 3.6556505829739097E-6 1.535373244849042E-4 5.8490409327582555E-5 76-77 2.3473352793092515 7.31130116594782E-5 3.6556505829739097E-6 1.5719297506787812E-4 6.031823461906951E-5 78-79 2.4834717070192003 7.31130116594782E-5 3.6556505829739097E-6 1.6084862565085204E-4 6.214605991055647E-5 80-81 2.618800235950311 8.042431282542602E-5 3.6556505829739097E-6 1.7364340269126073E-4 6.214605991055647E-5 82-83 2.7653424730446945 8.407996340839992E-5 3.6556505829739097E-6 1.7547122798274766E-4 6.214605991055647E-5 84-85 2.9400405309291435 8.407996340839992E-5 7.311301165947819E-6 1.7912687856572158E-4 6.214605991055647E-5 86-87 3.1398858091738697 9.139126457434775E-5 7.311301165947819E-6 1.900938303146433E-4 6.214605991055647E-5 88-89 3.3549733225243057 9.504691515732165E-5 7.311301165947819E-6 1.9192165560613026E-4 6.945736107650429E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 8200 0.0 75.58906 1 TCGACGG 8995 0.0 75.289734 2 ACGGCGC 13000 0.0 63.600563 5 GACGGCG 12245 0.0 58.05919 4 CGACGGC 13380 0.0 51.962845 3 CGGCGCG 16725 0.0 49.15048 6 TATCTCG 8140 0.0 41.832344 2 AATTCGA 5830 0.0 33.955627 1 ATCTCGA 15230 0.0 33.126266 1 TACGGCG 3380 0.0 32.45661 4 CGCGCCG 34845 0.0 30.447098 9 GGGCGCG 20640 0.0 29.197762 8 CTACGGC 3605 0.0 28.718328 3 TCTCGAG 18990 0.0 28.309938 2 GGCGCGC 37530 0.0 28.281067 7 TCGAGGG 20360 0.0 27.687319 4 GCGCGCC 38880 0.0 27.396849 8 CTCGAGG 20130 0.0 27.059988 3 CGAGGGC 22450 0.0 26.971148 5 GCGGTAC 3980 0.0 26.489458 9 >>END_MODULE