FastQCFastQC Report
Tue 31 May 2016
SRR1051273_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051273_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35379903
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT32249739.115268066167395No Hit
ATATGTATACATATGTATACATATGTATACATATGTATACATATGTATAC3392730.9589427082374986No Hit
ATACATATGTATACATATGTATACATATGTATACATATGTATACATATGT3215690.9089030006667909No Hit
ACATATGTATACATATGTATACATATGTATACATATGTATACATATGTAT3037200.8584534559068746No Hit
GTATACATATGTATACATATGTATACATATGTATACATATGTATACATAT2887010.8160028024949645No Hit
CATATGTATACATATGTATACATATGTATACATATGTATACATATGTATA2314180.6540945010504975No Hit
ATGTATACATATGTATACATATGTATACATATGTATACATATGTATACAT2285970.646121047872856No Hit
TATACATATGTATACATATGTATACATATGTATACATATGTATACATATG2209120.6243996768447896No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT2064380.5834894459716298No Hit
TATGTATACATATGTATACATATGTATACATATGTATACATATGTATACA1669610.47190915136200345No Hit
TACATATGTATACATATGTATACATATGTATACATATGTATACATATGTA1508470.42636352055572346No Hit
TGTATACATATGTATACATATGTATACATATGTATACATATGTATACATA1259570.3560128471805024No Hit
ATATATGTACATATATGTACATATATGTACATATATGTACATATATGTAC1201710.33965893009938436No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT1052860.29758702277957066No Hit
ACATATATGTACATATATGTACATATATGTACATATATGTACATATATGT891350.25193681282845803No Hit
ATATGTACATATATGTACATATATGTACATATATGTACATATATGTACAT779830.22041609328324047No Hit
CATATATGTACATATATGTACATATATGTACATATATGTACATATATGTA753540.21298532107337886No Hit
TATATGTACATATATGTACATATATGTACATATATGTACATATATGTACA604440.17084275216921876No Hit
GTACATATATGTACATATATGTACATATATGTACATATATGTACATATAT582100.1645284329920294No Hit
ATGTACATATATGTACATATATGTACATATATGTACATATATGTACATAT548280.15496933386165587No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT524140.14814625127717282No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT470010.1328466050345022No Hit
TACATATATGTACATATATGTACATATATGTACATATATGTACATATATG416080.1176034880593087No Hit
TATGTACATATATGTACATATATGTACATATATGTACATATATGTACATA409240.1156701871115927No Hit
ATATGTATACATATGTATACATATGTATACATATAGATCGGAAGAGCGTC369710.10449717739474866No Hit
ATACATATGTATACATATGTATACATATGTATAGATCGGAAGAGCGTCGT356050.10063622842606437No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG2030650.092.517311
ATATCTC4031350.091.672741
TATCTCG4023500.091.5878142
TCGACGG2077550.090.622442
ACGGCGC2118700.088.955645
GACGGCG2120600.088.7521744
ATCTCGA4159450.088.358633
CGGCGCG2140450.088.18156
CGACGGC2138300.088.001573
TCTCGAG4224950.086.3160554
CTCGAGG4299300.084.832585
TCGAGGG4320650.084.398156
CGAGGGC4343850.083.968937
AGGGCGC4388600.083.512629
GAGGGCG4393100.083.135868
AATTCGA96100.059.0546761
TATCGCG20400.054.596082
TCGCGAG20550.053.2686424
GCGGTAC49150.045.1807379
CTCGACG74950.044.8206375
Produced by FastQC (version 0.11.5)