FastQCFastQC Report
Tue 31 May 2016
SRR1051273_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051273_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35379903
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGTATACATATGTATACATATGTATACATATGTATACATATGTATAC3569411.0088806631267475No Hit
ATACATATGTATACATATGTATACATATGTATACATATGTATACATATGT3340780.9442592310103282No Hit
ACATATGTATACATATGTATACATATGTATACATATGTATACATATGTAT3256930.9205593356205639No Hit
GTATACATATGTATACATATGTATACATATGTATACATATGTATACATAT2982510.8429955277152682No Hit
CATATGTATACATATGTATACATATGTATACATATGTATACATATGTATA2520760.7124835814275693No Hit
ATGTATACATATGTATACATATGTATACATATGTATACATATGTATACAT2463100.6961861936139282No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2357620.6663726579465185No Hit
TATACATATGTATACATATGTATACATATGTATACATATGTATACATATG2353490.6652053285731168No Hit
TATGTATACATATGTATACATATGTATACATATGTATACATATGTATACA1714270.48453213678963447No Hit
TACATATGTATACATATGTATACATATGTATACATATGTATACATATGTA1653070.4672341809416493No Hit
TGTATACATATGTATACATATGTATACATATGTATACATATGTATACATA1345640.38034021743926205No Hit
ATATATGTACATATATGTACATATATGTACATATATGTACATATATGTAC1248910.35299983722397427No Hit
ACATATATGTACATATATGTACATATATGTACATATATGTACATATATGT938380.2652296700756924No Hit
CATATATGTACATATATGTACATATATGTACATATATGTACATATATGTA852610.24098709371814842No Hit
ATATGTACATATATGTACATATATGTACATATATGTACATATATGTACAT816810.23086835483975182No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT765770.21644208577960206No Hit
ATGTACATATATGTACATATATGTACATATATGTACATATATGTACATAT586960.16590209419172233No Hit
GTACATATATGTACATATATGTACATATATGTACATATATGTACATATAT570890.1613599675499393No Hit
TATATGTACATATATGTACATATATGTACATATATGTACATATATGTACA566230.16004283561772342No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT477880.13507103170972515No Hit
TATGTACATATATGTACATATATGTACATATATGTACATATATGTACATA446470.1261931102524504No Hit
TACATATATGTACATATATGTACATATATGTACATATATGTACATATATG443180.12526320380245248No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG111550.079.97821
TCGACGG123850.075.187842
ACGGCGC159200.061.0561145
GACGGCG164650.058.2852864
AATTCGA98950.056.2485431
CGACGGC188950.050.412523
CGGCGCG206900.047.0946166
AATTCCC223100.044.7809141
GCGGTAC58150.041.1561939
ATTCCCC255650.038.858132
GGCGGTA62950.038.3951578
ATTCGAG66050.036.0189442
ATCTCGA251750.032.9245871
TCTCGAG269100.031.7808912
ATATCTC179500.031.4879341
TCGAGGG291650.030.0555114
GGGCGCG292950.030.051818
CTCGAGG292950.029.7924613
TACGGCG41650.028.37018288-89
CGCGCCG463800.028.052679
Produced by FastQC (version 0.11.5)