FastQCFastQC Report
Tue 31 May 2016
SRR1051272_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051272_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6755160
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT2641003.9096039175978063No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT319570.47307539717786107No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT118010.1746960841786131No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC85040.12588895007668213No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA84230.12468986670930074No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC81960.12132947258096033No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA69120.10232178068321106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG434700.094.0274051
TCGACGG444950.091.904062
TATCTCG420750.091.5234452
ATATCTC424250.090.8579561
ACGGCGC461700.088.4464345
GACGGCG465500.087.785644
CGGCGCG467300.087.508486
CGACGGC467700.087.2914663
ATCTCGA443000.086.808683
TCTCGAG450600.084.395914
CTCGAGG464150.081.993525
TCGAGGG469350.081.034516
CGAGGGC472450.080.402277
AGGGCGC476750.079.846449
GAGGGCG483850.078.782758
CGCGCCG924700.045.2036939
GCGCGCC946050.044.24388
GGCGCGC946400.044.21747
CCATATT516450.039.93045818-19
GGGCGCG477350.039.91283810-11
Produced by FastQC (version 0.11.5)