##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051272_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6755160 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05664306989028 33.0 31.0 34.0 30.0 34.0 2 32.30252088773619 34.0 31.0 34.0 30.0 34.0 3 32.3806889844208 34.0 31.0 34.0 30.0 34.0 4 35.805830505865146 37.0 35.0 37.0 35.0 37.0 5 35.791407901515285 37.0 35.0 37.0 35.0 37.0 6 35.79518797482221 37.0 35.0 37.0 35.0 37.0 7 35.748469614339264 37.0 35.0 37.0 33.0 37.0 8 35.72944430627846 37.0 35.0 37.0 33.0 37.0 9 37.42866519815963 39.0 37.0 39.0 34.0 39.0 10-11 37.41293300232711 39.0 37.0 39.0 34.0 39.0 12-13 37.36513687314586 39.0 37.0 39.0 34.0 39.0 14-15 38.71182903143671 40.0 38.0 41.0 34.0 41.0 16-17 38.59297803456913 40.0 38.0 41.0 34.0 41.0 18-19 38.62024393204602 40.0 38.0 41.0 34.0 41.0 20-21 38.606302441392955 40.0 38.0 41.0 34.0 41.0 22-23 38.56556950538551 40.0 38.0 41.0 34.0 41.0 24-25 38.53688572883544 40.0 38.0 41.0 34.0 41.0 26-27 38.428260174444425 40.0 38.0 41.0 34.0 41.0 28-29 38.18756994652976 40.0 38.0 41.0 33.5 41.0 30-31 37.65273035723802 39.5 36.5 41.0 33.0 41.0 32-33 37.244682361335634 39.0 35.5 41.0 33.0 41.0 34-35 36.99965774311785 39.0 35.0 40.0 32.0 41.0 36-37 37.13847754901438 39.0 35.0 41.0 32.0 41.0 38-39 36.96628962156336 39.0 35.0 41.0 31.5 41.0 40-41 36.27035851112335 39.0 35.0 41.0 28.5 41.0 42-43 36.25040161891058 39.0 35.0 41.0 28.5 41.0 44-45 36.11335719657269 39.0 35.0 41.0 27.5 41.0 46-47 36.03115137169216 39.0 35.0 41.0 27.5 41.0 48-49 35.77620870564132 39.0 35.0 41.0 25.5 41.0 50-51 35.46580051397746 38.5 34.0 40.5 26.0 41.0 52-53 35.144483476335125 38.0 34.0 40.0 24.5 41.0 54-55 34.756452178778886 38.0 34.0 40.0 24.0 41.0 56-57 34.28993902439024 37.5 33.5 40.0 23.0 41.0 58-59 33.93178414722968 37.0 33.0 40.0 22.0 41.0 60-61 33.75762195121951 37.0 33.0 40.0 22.0 41.0 62-63 33.43203987766389 36.0 33.0 39.5 20.5 41.0 64-65 33.056103852462414 36.0 33.0 39.0 19.5 41.0 66-67 32.57807653704724 35.0 33.0 39.0 18.5 41.0 68-69 32.11661559459731 35.0 32.0 38.0 17.0 40.0 70-71 31.63918582239355 35.0 32.0 37.0 12.0 39.5 72-73 31.25547596207936 35.0 32.0 37.0 9.0 39.0 74-75 30.88508081229756 35.0 31.0 36.0 7.0 39.0 76-77 30.461439181307327 35.0 31.0 36.0 6.0 37.5 78-79 30.18535415889483 35.0 31.0 35.0 2.0 37.0 80-81 29.939122019315604 35.0 31.0 35.0 2.0 36.5 82-83 29.681341744681102 35.0 31.0 35.0 2.0 36.0 84-85 29.397115316291547 35.0 30.0 35.0 2.0 36.0 86-87 29.147029678053517 34.5 30.0 35.0 2.0 35.5 88-89 28.890969940016227 34.0 30.0 35.0 2.0 35.0 90-91 28.696879422545138 34.0 29.0 35.0 2.0 35.0 92-93 28.510748968196165 34.0 29.0 35.0 2.0 35.0 94-95 28.228342555912814 34.0 29.0 35.0 2.0 35.0 96-97 27.917909272319235 34.0 29.0 35.0 2.0 35.0 98-99 27.57304838079335 34.0 29.0 35.0 2.0 35.0 100-101 26.34580128375938 33.0 25.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3395546131705842 1101 2 0.3384405804700634 1101 3 0.3451659742191211 1101 4 0.3346825688903081 1101 5 0.364000185597277 1101 6 0.3360531359730743 1101 7 0.3617039913250366 1101 8 0.2789972369397802 1101 9 0.4039920319392536 1101 10-11 0.3122312749566305 1101 12-13 0.39829491963495656 1101 14-15 0.5253520167035859 1101 16-17 0.4603696703115503 1101 18-19 0.5055379621980265 1101 20-21 0.4838087771351738 1101 22-23 0.48052668090119965 1101 24-25 0.5161299438337963 1101 26-27 0.5536786505516247 1101 28-29 0.6679879830619626 1101 30-31 0.6679605617653976 1101 32-33 0.6926510464600923 1101 34-35 0.7270993099236591 1101 36-37 0.7560254779478157 1101 38-39 0.7314456692418503 1101 40-41 0.7304672678847481 1101 42-43 0.7354013045232151 1101 44-45 0.725647179309064 1101 46-47 0.7532950225951112 1101 48-49 0.656481608956824 1101 50-51 0.6369639006319119 1101 52-53 0.5954019626044769 1101 54-55 0.7271898387149989 1101 56-57 0.7011250729975984 1101 58-59 0.6942052955643945 1101 60-61 0.6525896944455312 1101 62-63 0.6661967020947301 1101 64-65 0.6442972316540789 1101 66-67 0.6248625568706032 1101 68-69 0.6117890158478261 1101 70-71 0.6183061995512311 1101 72-73 0.7093005106252726 1101 74-75 0.6748671633541825 1101 76-77 0.5535770548261141 1101 78-79 0.45741944924822064 1101 80-81 0.46286403899558337 1101 82-83 0.5157744986495523 1101 84-85 0.5232831979462524 1101 86-87 0.5820618319757713 1101 88-89 0.6104505553501838 1101 90-91 0.6258340902523898 1101 92-93 0.621720392776826 1101 94-95 0.6159577493294819 1101 96-97 0.6097576217369962 1101 98-99 0.66094008105161 1101 100-101 0.8085489238318075 1102 1 0.36816038676270324 1102 2 0.37114257796513783 1102 3 0.38153954418101677 1102 4 0.3037534235767012 1102 5 0.30926951572500627 1102 6 0.28666324450831127 1102 7 0.3277765557064072 1102 8 0.2718727216669521 1102 9 0.35836760883601926 1102 10-11 0.3506544941146501 1102 12-13 0.3769919197088498 1102 14-15 0.5356408509016859 1102 16-17 0.45579658444162163 1102 18-19 0.542315734523136 1102 20-21 0.4605338580446059 1102 22-23 0.46746106839260904 1102 24-25 0.4572499531326386 1102 26-27 0.5406293216788569 1102 28-29 0.5655632288164014 1102 30-31 0.6327352701635718 1102 32-33 0.5966417843875433 1102 34-35 0.6412138823910993 1102 36-37 0.660407624395944 1102 38-39 0.6828477160099595 1102 40-41 0.6084275230071157 1102 42-43 0.6123408323003829 1102 44-45 0.6072239512251016 1102 46-47 0.5352017423792859 1102 48-49 0.5767709367468257 1102 50-51 0.5573680382820783 1102 52-53 0.5128069235545212 1102 54-55 0.504395332750498 1102 56-57 0.5203560338832958 1102 58-59 0.45666739233373477 1102 60-61 0.4301899814193533 1102 62-63 0.5052053009143975 1102 64-65 0.45112882991859493 1102 66-67 0.45960449288654814 1102 68-69 0.4278952139643053 1102 70-71 0.49113415577325625 1102 72-73 0.534244204236245 1102 74-75 0.5230404118422705 1102 76-77 0.39370384416105964 1102 78-79 0.29890036205937776 1102 80-81 0.43298704776245955 1102 82-83 0.46733562544417495 1102 84-85 0.37002688949558404 1102 86-87 0.4155784631373578 1102 88-89 0.510833485470755 1102 90-91 0.514825042681256 1102 92-93 0.4291823357287221 1102 94-95 0.505151602555113 1102 96-97 0.49505871902532306 1102 98-99 0.5408635308552121 1102 100-101 0.6764585773854002 1103 1 0.2719135331209017 1103 2 0.26876161195645665 1103 3 0.2920650404539913 1103 4 0.2440881923184932 1103 5 0.2876290036186546 1103 6 0.274543743699212 1103 7 0.2769512957790994 1103 8 0.2708278497873451 1103 9 0.33291825498786665 1103 10-11 0.32643072432020404 1103 12-13 0.3387148235748185 1103 14-15 0.44826462813686874 1103 16-17 0.45949868985272957 1103 18-19 0.49032532099294457 1103 20-21 0.42644724246192567 1103 22-23 0.4507736245530367 1103 24-25 0.4671261597713041 1103 26-27 0.43868272526493257 1103 28-29 0.43603719388480755 1103 30-31 0.5133735093013811 1103 32-33 0.5032120742942681 1103 34-35 0.508316776565934 1103 36-37 0.4678898414617336 1103 38-39 0.34420969066847107 1103 40-41 0.40233381287530534 1103 42-43 0.4289279011185343 1103 44-45 0.42577281364380326 1103 46-47 0.397645612044073 1103 48-49 0.44786369430052986 1103 50-51 0.36870868214128905 1103 52-53 0.3904371940608087 1103 54-55 0.3712176206750186 1103 56-57 0.35416083545836585 1103 58-59 0.35803118936001255 1103 60-61 0.33283015701170626 1103 62-63 0.44876174375609423 1103 64-65 0.45953345500097953 1103 66-67 0.41513374541527526 1103 68-69 0.38522171664944693 1103 70-71 0.438487859628129 1103 72-73 0.4658715398071607 1103 74-75 0.3470203322045613 1103 76-77 0.4165797664799271 1103 78-79 0.3868432910021866 1103 80-81 0.4025751373427724 1103 82-83 0.4697471113147422 1103 84-85 0.40963678582356167 1103 86-87 0.4418075472455527 1103 88-89 0.5351756631760374 1103 90-91 0.5641852992695462 1103 92-93 0.5364828685092426 1103 94-95 0.5797641163151432 1103 96-97 0.5273097025270097 1103 98-99 0.6201671936520121 1103 100-101 0.7432696122145508 1104 1 0.24403413241018512 1104 2 0.22776410858595852 1104 3 0.18578680652225188 1104 4 0.1703163686332516 1104 5 0.1654739696281453 1104 6 0.1757610244816945 1104 7 0.22251058552405567 1104 8 0.16599430251392278 1104 9 0.1926878784711903 1104 10-11 0.20345373460233418 1104 12-13 0.23294078363874604 1104 14-15 0.32741452906549995 1104 16-17 0.4003454118319354 1104 18-19 0.3181489278492222 1104 20-21 0.36381403190659967 1104 22-23 0.3820640008769445 1104 24-25 0.37162692161747657 1104 26-27 0.3851968516760209 1104 28-29 0.3529052827916246 1104 30-31 0.411266141048209 1104 32-33 0.39387126490874635 1104 34-35 0.42595494637293285 1104 36-37 0.44420023096667194 1104 38-39 0.38722105461998524 1104 40-41 0.3357757669895918 1104 42-43 0.36736794482069257 1104 44-45 0.3040389614227692 1104 46-47 0.30743872582215914 1104 48-49 0.23175665934653722 1104 50-51 0.1775496086884374 1104 52-53 0.24366955500558873 1104 54-55 0.2710288310843225 1104 56-57 0.2516063366560388 1104 58-59 0.22405917430621258 1104 60-61 0.11453196186469938 1104 62-63 0.26603289234115124 1104 64-65 0.22838777807449162 1104 66-67 0.2823458267124508 1104 68-69 0.29556402844998075 1104 70-71 0.33539848975769715 1104 72-73 0.3334076625998321 1104 74-75 0.2854630753526983 1104 76-77 0.39144543522828457 1104 78-79 0.3296144338132656 1104 80-81 0.31529331395487503 1104 82-83 0.353710823441002 1104 84-85 0.3566497414366232 1104 86-87 0.2917688332120605 1104 88-89 0.3434338390079681 1104 90-91 0.46162441272556975 1104 92-93 0.31465357638103697 1104 94-95 0.40832657574388875 1104 96-97 0.45817073623000937 1104 98-99 0.5053087735128337 1104 100-101 0.5649891238419222 1105 1 0.0927010750060333 1105 2 0.0670476257782937 1105 3 0.09572182132036033 1105 4 -0.04858082280363618 1105 5 0.06877778957296243 1105 6 0.043624013921501614 1105 7 0.04647023063367328 1105 8 0.09463617663548263 1105 9 0.1097151269043195 1105 10-11 0.017022507027164124 1105 12-13 0.12566098220374755 1105 14-15 0.18045295118588456 1105 16-17 0.018658316761552385 1105 18-19 0.13373128955234392 1105 20-21 0.09818633298633728 1105 22-23 0.049050512059118034 1105 24-25 0.08558750735723919 1105 26-27 0.14825109239702527 1105 28-29 0.18653197661205922 1105 30-31 0.23107205177981172 1105 32-33 0.13093791001948318 1105 34-35 0.19747954294309977 1105 36-37 0.17841484029415966 1105 38-39 0.2031538516111624 1105 40-41 0.07117927358776655 1105 42-43 0.013278872057895796 1105 44-45 0.00726918190870407 1105 46-47 0.09335772374026874 1105 48-49 0.13045291872997922 1105 50-51 0.14346341559940612 1105 52-53 0.04007620237565135 1105 54-55 0.15715868554998025 1105 56-57 0.2046707808820969 1105 58-59 0.16271669058285454 1105 60-61 0.04644986730631473 1105 62-63 0.11836436631683966 1105 64-65 0.03720708025900876 1105 66-67 -0.01130335349268563 1105 68-69 0.1416302360170434 1105 70-71 0.0726085046084286 1105 72-73 0.2016790770314465 1105 74-75 0.18449961666681247 1105 76-77 0.32615523535201874 1105 78-79 0.31444385266274466 1105 80-81 0.22633828470185335 1105 82-83 0.2818692625794057 1105 84-85 0.19155632580221038 1105 86-87 0.2102559827556192 1105 88-89 0.26521732658634534 1105 90-91 0.35620010123773227 1105 92-93 0.27630895273442135 1105 94-95 0.3900278937693713 1105 96-97 0.2770574069726024 1105 98-99 0.3042622110413511 1105 100-101 0.3858576030287182 1106 1 -0.1149654964637783 1106 2 -0.14665458175998225 1106 3 -0.1515004542516607 1106 4 -0.3150077198664789 1106 5 -0.10315174351637779 1106 6 -0.1096409841335344 1106 7 -0.1161703567089063 1106 8 -0.07848462706680692 1106 9 -0.12097954193457383 1106 10-11 -0.11520372736006124 1106 12-13 -0.11559242478804066 1106 14-15 -0.27626591752012786 1106 16-17 -0.20474400297785422 1106 18-19 -0.27243896497060405 1106 20-21 -0.35351634271159327 1106 22-23 -0.1360393663878341 1106 24-25 -0.11264938767276789 1106 26-27 -0.40402396893919956 1106 28-29 -0.23939236761527383 1106 30-31 -0.20518052260815267 1106 32-33 -0.2691348096579418 1106 34-35 -0.1719710501739229 1106 36-37 -0.3917617889136622 1106 38-39 -0.14470132258269786 1106 40-41 -0.10438378841439544 1106 42-43 -0.13312846497404252 1106 44-45 -0.21427102170916612 1106 46-47 -0.0961286854812684 1106 48-49 0.009927207918529746 1106 50-51 -0.1619173391282942 1106 52-53 -0.1874640772852061 1106 54-55 -0.10329827159930716 1106 56-57 -0.038033807826792554 1106 58-59 0.03614072889463671 1106 60-61 0.02743240601306951 1106 62-63 0.12413330327849792 1106 64-65 0.05837318945933134 1106 66-67 -0.008215905298150972 1106 68-69 0.15443038783235608 1106 70-71 0.19453521796579665 1106 72-73 0.12409618823204482 1106 74-75 0.17701898181914544 1106 76-77 0.23127085329786468 1106 78-79 0.023875309164086644 1106 80-81 0.026512814877083457 1106 82-83 0.08179399642507335 1106 84-85 0.08819586457404327 1106 86-87 0.14626630785852512 1106 88-89 0.23721784353287134 1106 90-91 0.23011492515468746 1106 92-93 0.10906601066538357 1106 94-95 0.1129046355947132 1106 96-97 -0.016078029494757118 1106 98-99 0.12449228980629812 1106 100-101 0.14483620693200905 1107 1 -0.24167317926388066 1107 2 -0.30352144926133207 1107 3 -0.3416709499385391 1107 4 -0.2065364724658565 1107 5 -0.32397879351683656 1107 6 -0.17139119833690586 1107 7 -0.21172137739574026 1107 8 -0.22081560578619985 1107 9 -0.21033541970901837 1107 10-11 -0.2278220133171942 1107 12-13 -0.18167677107432922 1107 14-15 -0.2428479431089272 1107 16-17 -0.31235115497496224 1107 18-19 -0.32160928284270796 1107 20-21 -0.2947909407065339 1107 22-23 -0.24716956111038257 1107 24-25 -0.3541098870662367 1107 26-27 -0.26703413554187705 1107 28-29 -0.34450830121238596 1107 30-31 -0.21672960499315508 1107 32-33 -0.2551267034993998 1107 34-35 -0.431408317518418 1107 36-37 -0.31831009844825786 1107 38-39 -0.2888429605728291 1107 40-41 -0.36595758921777133 1107 42-43 -0.32086925581923254 1107 44-45 -0.2604399036112568 1107 46-47 -0.27781689253520625 1107 48-49 -0.1951923140193017 1107 50-51 -0.2589968064310426 1107 52-53 -0.12075619551813332 1107 54-55 -0.08119988001414669 1107 56-57 -0.05705465618203931 1107 58-59 -0.07923355459258374 1107 60-61 -0.10829506920637044 1107 62-63 -0.07041813673363606 1107 64-65 -0.19179103900694372 1107 66-67 -0.10073514276821527 1107 68-69 -0.04254560946839092 1107 70-71 0.09419301509359101 1107 72-73 0.09863404109546181 1107 74-75 -0.004635739236146463 1107 76-77 -0.3251473089960335 1107 78-79 -0.32187045463587793 1107 80-81 -0.1324564065698013 1107 82-83 -0.05516379181019104 1107 84-85 -0.19436861743642098 1107 86-87 -0.19434342954942707 1107 88-89 -0.12343317737039072 1107 90-91 -0.07450419722900392 1107 92-93 -0.16607850374871802 1107 94-95 -0.09107312798036205 1107 96-97 -0.14648344300355376 1107 98-99 -0.22992905593530466 1107 100-101 -0.24902694604481113 1108 1 -0.4785316125653907 1108 2 -0.41923779991166654 1108 3 -0.41843901509299286 1108 4 -0.5047588129076956 1108 5 -0.7362387863139475 1108 6 -0.5100059314868233 1108 7 -0.5555821804505001 1108 8 -1.3014353698030234 1108 9 -0.7902433457859956 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0.10577743173983833 2305 12-13 0.161168039950077 2305 14-15 0.2353122742157865 2305 16-17 0.23775372451964216 2305 18-19 0.166140678515724 2305 20-21 0.18921201977291702 2305 22-23 0.2145301355673439 2305 24-25 0.19611037826059174 2305 26-27 0.16645105380816716 2305 28-29 0.12284677493896368 2305 30-31 0.0851998812970507 2305 32-33 0.20465698876769522 2305 34-35 0.18868165998360098 2305 36-37 0.07426038423918868 2305 38-39 0.22580893735838004 2305 40-41 0.07662618618854111 2305 42-43 0.10468084419487411 2305 44-45 0.1777952188902745 2305 46-47 0.1287491796107929 2305 48-49 0.0821762292306758 2305 50-51 0.14378929148515596 2305 52-53 0.06263432769581101 2305 54-55 0.02945473718580871 2305 56-57 0.18171714472151024 2305 58-59 0.1438465561637443 2305 60-61 0.22091192124431558 2305 62-63 0.19987189405362926 2305 64-65 0.11069949354362052 2305 66-67 0.15558642332619144 2305 68-69 0.06851845488186115 2305 70-71 0.13272781830592706 2305 72-73 0.04729686977055181 2305 74-75 0.13346652998145103 2305 76-77 0.24465173845750599 2305 78-79 0.2235527347319035 2305 80-81 0.18260758104279518 2305 82-83 0.11766437140027364 2305 84-85 0.10463421837292941 2305 86-87 0.13028063465935702 2305 88-89 0.18298243766831845 2305 90-91 0.19755334901278587 2305 92-93 0.21234064423133603 2305 94-95 0.2913719757346023 2305 96-97 0.35285079928657126 2305 98-99 0.2739837684990647 2305 100-101 0.40854734827489025 2306 1 -0.3336018381328998 2306 2 -0.5351503955455819 2306 3 -0.3809567213358207 2306 4 -0.2645253904633478 2306 5 -0.2703052037764593 2306 6 -0.28372187508532676 2306 7 -0.15896657207257903 2306 8 -0.15695294642542734 2306 9 -0.1888080008010533 2306 10-11 -0.14473662949057342 2306 12-13 -0.1626085418581411 2306 14-15 -0.3020121650350376 2306 16-17 -0.21880234899804663 2306 18-19 -0.17630401881784508 2306 20-21 -0.19608623635500777 2306 22-23 -0.24116728008230126 2306 24-25 -0.2506884377688152 2306 26-27 -0.1436656756781005 2306 28-29 -0.13583920287868523 2306 30-31 -0.22077638076967077 2306 32-33 -0.2018081945676613 2306 34-35 -0.060142601330618106 2306 36-37 -0.33566886146201824 2306 38-39 -0.27581007287390946 2306 40-41 -0.24852231758080023 2306 42-43 -0.21490296048641255 2306 44-45 -0.07398193075192694 2306 46-47 -0.19041443648256973 2306 48-49 -0.18143097800294328 2306 50-51 -0.32501314459764785 2306 52-53 -0.07098161869051722 2306 54-55 -0.14240796065200811 2306 56-57 -0.09435689187775154 2306 58-59 -0.06353545370645008 2306 60-61 -0.06219253127709834 2306 62-63 -0.05775959258339469 2306 64-65 -0.0964864205419218 2306 66-67 -0.09063687607268633 2306 68-69 -0.09985409864532357 2306 70-71 -0.39315566844588545 2306 72-73 -0.3101793442346157 2306 74-75 -0.17369264014647356 2306 76-77 -0.06955805307980256 2306 78-79 -0.1345546467831582 2306 80-81 -0.08480362388511509 2306 82-83 0.0174212114607748 2306 84-85 -0.07615253887394502 2306 86-87 -0.20508100652432404 2306 88-89 -0.3192451334534425 2306 90-91 -0.2969294496189683 2306 92-93 -0.14095261104579393 2306 94-95 0.005631054662242008 2306 96-97 0.05560034760133803 2306 98-99 0.07315696404455352 2306 100-101 0.17438690945428803 2307 1 -0.35447535051195445 2307 2 -0.30901175890419097 2307 3 -0.4837600356055525 2307 4 -0.25303392904020683 2307 5 -0.34448766017371213 2307 6 -0.280631545237469 2307 7 -0.16322393214467468 2307 8 -0.18199814470959552 2307 9 -0.16652671094105642 2307 10-11 -0.39243004742053245 2307 12-13 -0.25690528250423483 2307 14-15 -0.4553001834849937 2307 16-17 -0.5328564067990627 2307 18-19 -0.5290418183332832 2307 20-21 -0.6748863559428955 2307 22-23 -0.6558659443359076 2307 24-25 -0.4478738954492414 2307 26-27 -0.34930275022529145 2307 28-29 -0.34675487125473836 2307 30-31 -0.25651569588496415 2307 32-33 -0.2881387459702722 2307 34-35 -0.20755987490490924 2307 36-37 -0.35137327085013936 2307 38-39 -0.3383997769212783 2307 40-41 -0.2532232247748354 2307 42-43 -0.4217818788804877 2307 44-45 -0.425406423059961 2307 46-47 -0.24397166473498544 2307 48-49 -0.2951869398900726 2307 50-51 -0.19016178331540345 2307 52-53 -0.23203583842532538 2307 54-55 -0.3316656747929798 2307 56-57 -0.2492862762714836 2307 58-59 -0.2551003995034833 2307 60-61 -0.2759513753684999 2307 62-63 -0.1515182668925874 2307 64-65 -0.23394634130702485 2307 66-67 -0.3421500377621385 2307 68-69 -0.3805019140528394 2307 70-71 -0.32302924939630984 2307 72-73 -0.3836367202598332 2307 74-75 -0.3358714890455232 2307 76-77 -0.6811200737820897 2307 78-79 -0.6208500413446458 2307 80-81 -0.44914108538802466 2307 82-83 -0.34776213561679015 2307 84-85 -0.2915029932896154 2307 86-87 -0.36621355476502515 2307 88-89 -0.3852607052227057 2307 90-91 -0.31477685218164453 2307 92-93 -0.33037503083629005 2307 94-95 -0.25248172300975114 2307 96-97 -0.3353028373331526 2307 98-99 -0.3453273451517731 2307 100-101 -0.2600243368337978 2308 1 -0.5081910588051421 2308 2 -0.43648167165321183 2308 3 -0.3894163305004241 2308 4 -0.6727735181575625 2308 5 -0.43016221967891255 2308 6 -0.7592510804791175 2308 7 -1.4024373907001575 2308 8 -0.5489643986353769 2308 9 -0.6249532470395778 2308 10-11 -0.4054313758016548 2308 12-13 -0.33963254473913196 2308 14-15 -0.9469195261867256 2308 16-17 -1.2452742655476996 2308 18-19 -0.7146544302902313 2308 20-21 -0.5529251496498446 2308 22-23 -0.5395809022501084 2308 24-25 -0.5869635188759688 2308 26-27 -0.5731800449931157 2308 28-29 -0.8870230503067376 2308 30-31 -1.4847255688734577 2308 32-33 -0.717127903263389 2308 34-35 -0.9998943946551435 2308 36-37 -1.310215939634439 2308 38-39 -0.8047015943315188 2308 40-41 -0.8513747077797333 2308 42-43 -0.6360449755184732 2308 44-45 -0.8850604467243528 2308 46-47 -1.1270446331899109 2308 48-49 -1.826904431363225 2308 50-51 -2.655802743154581 2308 52-53 -1.8297198945962165 2308 54-55 -1.3885079647897172 2308 56-57 -0.6463602353935443 2308 58-59 -0.7012236839023771 2308 60-61 -0.15054213245500847 2308 62-63 -0.11050858669082686 2308 64-65 -0.1871513034587764 2308 66-67 -0.22466210357633543 2308 68-69 -0.6149307549290235 2308 70-71 -0.860688066795209 2308 72-73 -0.9330967593980866 2308 74-75 -0.9190636577309981 2308 76-77 -0.6590954902750177 2308 78-79 -0.32523578140456877 2308 80-81 -0.5547678040179278 2308 82-83 -0.5222566366529229 2308 84-85 -0.817288249599315 2308 86-87 -0.623657298633649 2308 88-89 -0.7437269182400534 2308 90-91 -1.116391036962149 2308 92-93 -0.7534015649636991 2308 94-95 -1.6435788579091373 2308 96-97 -1.0697905813082222 2308 98-99 -1.552291893733976 2308 100-101 -1.7843226755586947 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 108.0 11 916.0 12 2387.0 13 4835.0 14 7704.0 15 11679.0 16 16777.0 17 27189.0 18 57889.0 19 156705.0 20 161525.0 21 102794.0 22 86837.0 23 80551.0 24 81092.0 25 85548.0 26 96507.0 27 107803.0 28 117410.0 29 127373.0 30 143050.0 31 163729.0 32 196584.0 33 249958.0 34 348702.0 35 530797.0 36 921123.0 37 1554739.0 38 1170854.0 39 141908.0 40 86.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 26.578704279395303 30.8875289408393 21.93225623079246 20.601510548972936 2 23.1637296525915 24.687438935569254 35.123638818325546 17.0251925935137 3 22.39753018433316 29.282740897328857 22.015585123076285 26.304143795261698 4 29.6266853782886 22.189067320389157 27.207601300339295 20.976646000982953 5 23.188022193404745 28.16001397450246 21.01965016372669 27.63231366836611 6 23.005628290077514 21.44331148336975 26.42944060540387 29.12161962114887 7 29.512802065384093 21.672528852018306 20.88406492222242 27.93060416037518 8 34.778732109972225 21.41047732400121 21.314905938571403 22.49588462745516 9 22.242759608950788 26.789713345057702 21.685748376056228 29.281778669935278 10-11 31.43690897032787 21.407916318784455 21.468817911048742 25.68635679983894 12-13 28.40590748405663 21.554752219044403 21.552657524026078 28.486682772872886 14-15 28.235682056383567 21.641411898459843 21.754902918657738 28.368003126498852 16-17 28.246074112234204 24.81167729557849 21.737775271052055 25.20447332113525 18-19 24.921223183462715 21.709219322710343 24.97584069067202 28.393716803154923 20-21 24.722005104246236 27.691897749276105 22.200354987890737 25.385742158586915 22-23 21.75989169760598 24.691783762338716 28.492530154726165 25.05579438532914 24-25 21.77545757613439 21.62797032194648 31.63840678829221 24.958165313626914 26-27 21.682480012683637 21.661296203390624 34.77176480968102 21.884458974244723 28-29 21.74164638587391 21.535973981371278 34.9355677733762 21.78681185937861 30-31 21.702221932393762 21.417223671487896 34.96638429857437 21.914170097543973 32-33 21.703260790555063 21.453030323890975 35.01206480217766 21.831644083376304 34-35 21.7520463545777 21.588154422291673 34.900247384886676 21.759551838243947 36-37 21.766230974667135 21.498364295279103 34.805044441264926 21.930360288788837 38-39 21.896626930683013 21.60252454474012 34.23410840078191 22.26674012379495 40-41 22.852784576898923 22.420183811653605 31.35117938557131 23.375852225876166 42-43 23.009624205005494 22.52208900977713 31.439787671338838 23.028499113878542 44-45 23.100113765221714 23.022019999258294 30.959203890683234 22.918662344836754 46-47 22.96568015347285 23.51284686719059 30.66807884032603 22.85339413901053 48-49 22.959483143129994 23.795135821779535 30.11696724523359 23.12841378985688 50-51 22.96747091882408 24.15614367552429 29.657410816419493 23.21897458923214 52-53 23.033330493474455 24.349549569021192 29.372450649926744 23.244669287577608 54-55 23.260688800853753 24.19986377762326 28.947129226657488 23.592318194865502 56-57 23.33399949075966 24.128584667128536 28.792286193073146 23.74512964903866 58-59 23.504289320000296 24.14535576560106 28.51081027068237 23.839544643716277 60-61 23.649153120590615 24.11323049830251 28.179351569220014 24.058264811886858 62-63 23.830286773370283 24.167961972773405 27.82589161470639 24.175859639149923 64-65 23.953799761959747 24.337543448267695 27.37456255662338 24.334094233149177 66-67 24.06039919729463 24.35535907682961 27.010316268047696 24.573925457828064 68-69 24.185486561426707 24.606456771516534 26.689952490494285 24.518104176562474 70-71 24.41189491894353 24.84812021259358 26.34292391898373 24.397060949479158 72-73 24.538760560081723 24.867717935741766 26.12644140910293 24.46708009507358 74-75 24.551981023049603 25.00431721624416 25.949434128236533 24.4942676324697 76-77 24.56894439002431 25.140303997547765 25.774428162828013 24.51632344959992 78-79 24.66667575122375 24.968562001509618 25.80059881055499 24.56416343671164 80-81 24.64437835284806 25.01275565177197 25.797274518954566 24.545591476425408 82-83 24.71425743943223 25.079195496037894 25.675839315339964 24.53070774918991 84-85 24.701826851101323 25.006833732205187 25.59981590188204 24.691523514811454 86-87 24.710067363571355 25.079730966140122 25.616017798804318 24.5941838714842 88-89 24.781512972654525 25.145529099861353 25.496976611345758 24.57598131613836 90-91 24.933332445123433 24.974286323343932 25.378229383167834 24.714151848364803 92-93 24.882171554781827 25.07021299273444 25.337186683957153 24.71042876852658 94-95 24.945624871903412 25.125687317532908 25.1721777282288 24.756510082334877 96-97 24.908298013800934 25.07233046439393 25.07823000695361 24.941141514851527 98-99 24.839946452175347 25.128344092885314 25.119691349462325 24.912018105477017 100-101 24.92983508747465 25.32332705220619 24.68039602928548 25.066441831033682 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 604.0 1 367.5 2 128.5 3 116.0 4 103.5 5 125.0 6 144.5 7 145.5 8 150.5 9 146.0 10 155.5 11 163.5 12 203.0 13 338.0 14 554.5 15 962.0 16 13266.5 17 19020.0 18 12751.0 19 12033.0 20 11548.5 21 11835.0 22 12307.5 23 13659.0 24 15402.5 25 17456.0 26 20259.0 27 23913.0 28 27756.0 29 32647.5 30 38984.5 31 44762.0 32 51522.5 33 61432.5 34 71365.5 35 80998.5 36 94291.5 37 106936.5 38 119923.5 39 134846.5 40 148375.0 41 159756.5 42 173537.5 43 189870.0 44 207466.5 45 227649.5 46 244689.5 47 263327.5 48 279683.5 49 287475.5 50 288221.0 51 289130.0 52 294700.5 53 299400.0 54 298764.5 55 290580.5 56 274352.5 57 250020.0 58 225772.5 59 203081.5 60 177362.5 61 150031.5 62 122533.5 63 93751.5 64 67981.0 65 48992.5 66 34621.5 67 24871.0 68 18485.5 69 14140.0 70 11319.0 71 9331.5 72 8168.0 73 6455.5 74 4801.0 75 4215.5 76 3457.0 77 2234.0 78 1351.5 79 836.5 80 486.5 81 326.5 82 218.5 83 145.0 84 88.5 85 49.0 86 17.5 87 2.5 88 1.5 89 0.5 90 0.5 91 0.0 92 0.5 93 1.5 94 1.0 95 0.5 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.4803498362733082E-5 28-29 0.0 30-31 0.003056922411904381 32-33 0.005728953866377702 34-35 0.001465546337910575 36-37 0.0017838215527093363 38-39 0.0029532979233652494 40-41 0.002990306669272082 42-43 0.0023759614872186593 44-45 0.00680220749767585 46-47 0.0013175113542832442 48-49 3.108734656173947E-4 50-51 0.0027978611905565523 52-53 0.0019170530379739338 54-55 4.2930145251925936E-4 56-57 0.0 58-59 1.480349836273308E-4 60-61 5.403276902397574E-4 62-63 0.0 64-65 0.0 66-67 5.921399345093233E-5 68-69 0.006017622084450998 70-71 0.0058251766057354675 72-73 0.0026868349528360543 74-75 0.0033825993758845093 76-77 0.03175350398806246 78-79 0.05589800981768012 80-81 0.04812617317724525 82-83 6.069434328720563E-4 84-85 9.844326411217498E-4 86-87 0.0014729480870919415 88-89 0.003545437857874573 90-91 0.0 92-93 0.0 94-95 5.181224426956578E-5 96-97 0.005906595846730499 98-99 0.001139869373930447 100-101 4.441049508819925E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6755160.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.93771817916962 #Duplication Level Percentage of deduplicated Percentage of total 1 82.81288545965543 43.011123066074376 2 9.02428051539355 9.374010763565636 3 2.567894142565832 4.001116868715738 4 1.266652193625416 2.6314809865417543 5 0.7286122657433439 1.8921229260032018 6 0.4974370756197814 1.5501447987246297 7 0.3891899113540563 1.4149545153858087 8 0.30586840237606233 1.2708885506016625 9 0.24779814060518107 1.158306299286673 >10 1.9691596711774 19.325026612275533 >50 0.15220165520221646 5.450655569908107 >100 0.03620983932025142 2.653229335081996 >500 8.910068650027631E-4 0.32804082952557045 >1k 6.323078419767927E-4 0.6226582775219637 >5k 2.011888581719102E-4 0.7206726186760424 >10k+ 8.622379635939008E-5 4.5955679821112945 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 264100 3.9096039175978063 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT 31957 0.47307539717786107 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11801 0.1746960841786131 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC 8504 0.12588895007668213 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA 8423 0.12468986670930074 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC 8196 0.12132947258096033 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA 6912 0.10232178068321106 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.960699672546616E-5 0.0 0.0 0.0 2.960699672546616E-5 2 2.960699672546616E-5 0.0 0.0 0.0 2.960699672546616E-5 3 2.960699672546616E-5 0.0 0.0 0.0 2.960699672546616E-5 4 2.960699672546616E-5 0.0 0.0 0.0 4.441049508819924E-5 5 4.441049508819924E-5 0.0 0.0 0.0 7.40174918136654E-5 6 5.921399345093232E-5 1.480349836273308E-5 0.0 0.0 7.40174918136654E-5 7 5.921399345093232E-5 1.480349836273308E-5 0.0 1.480349836273308E-5 1.1842798690186464E-4 8 7.40174918136654E-5 1.480349836273308E-5 0.0 1.480349836273308E-5 1.3323148526459772E-4 9 7.40174918136654E-5 1.480349836273308E-5 0.0 2.960699672546616E-5 1.6283848199006388E-4 10-11 9.622273935776502E-5 1.480349836273308E-5 0.0 2.960699672546616E-5 1.9984722789689657E-4 12-13 1.5543673280869736E-4 2.960699672546616E-5 0.0 2.960699672546616E-5 2.3685597380372929E-4 14-15 1.9244547871553004E-4 2.960699672546616E-5 0.0 2.960699672546616E-5 2.73864719710562E-4 16-17 3.256769639801278E-4 2.960699672546616E-5 0.0 2.960699672546616E-5 3.108734656173947E-4 18-19 5.47729439421124E-4 3.70087459068327E-5 0.0 2.960699672546616E-5 3.5528396070559393E-4 20-21 8.956116509453514E-4 4.441049508819924E-5 0.0 4.441049508819924E-5 3.7008745906832704E-4 22-23 0.0014951533346360412 4.441049508819924E-5 0.0 6.661574263229886E-5 4.070962049751597E-4 24-25 0.0025387999692087233 4.441049508819924E-5 0.0 7.40174918136654E-5 5.107206935142912E-4 26-27 0.0037452850857714695 5.181224426956578E-5 0.0 8.882099017639848E-5 5.551311886024906E-4 28-29 0.004885154459701916 5.921399345093232E-5 0.0 1.0362448853913156E-4 5.847381853279568E-4 30-31 0.006106443074627396 5.921399345093232E-5 0.0 1.0362448853913156E-4 6.291486804161559E-4 32-33 0.007608998158444804 5.921399345093232E-5 0.0 1.0362448853913156E-4 6.957644230484547E-4 34-35 0.009155963737350411 5.921399345093232E-5 0.0 1.0362448853913156E-4 7.475766673180205E-4 36-37 0.010725134563800118 5.921399345093232E-5 0.0 1.0362448853913156E-4 7.845854132248533E-4 38-39 0.012235091396798892 5.921399345093232E-5 0.0 1.0362448853913156E-4 8.21594159131686E-4 40-41 0.013493388757631203 5.921399345093232E-5 0.0 1.258297360832312E-4 8.808081525826183E-4 42-43 0.014944131597179045 5.921399345093232E-5 0.0 1.5543673280869736E-4 9.104151493080845E-4 44-45 0.016676140905618815 5.921399345093232E-5 0.0 1.6283848199006388E-4 9.326203968521841E-4 46-47 0.01827491872879399 5.921399345093232E-5 7.40174918136654E-6 1.6283848199006388E-4 9.992361394844831E-4 48-49 0.019666447574890895 5.921399345093232E-5 2.960699672546616E-5 1.6283848199006388E-4 0.0010362448853913158 50-51 0.02065088021601265 5.921399345093232E-5 2.960699672546616E-5 1.6283848199006388E-4 0.0010732536312981484 52-53 0.022057212560472293 7.40174918136654E-5 2.960699672546616E-5 1.7764198035279696E-4 0.001110262377204981 54-55 0.023952060350902124 7.40174918136654E-5 2.960699672546616E-5 1.8504372953416352E-4 0.00117687811983728 56-57 0.025809899395425127 8.882099017639848E-5 2.960699672546616E-5 1.9244547871553004E-4 0.0012508956116509453 58-59 0.0275419087038649 8.882099017639848E-5 2.960699672546616E-5 1.9244547871553004E-4 0.0013027078559205113 60-61 0.029088874282770502 8.882099017639848E-5 2.960699672546616E-5 2.0724897707826312E-4 0.0013471183510087104 62-63 0.03093190982893077 8.882099017639848E-5 2.960699672546616E-5 2.2945422462236273E-4 0.0014137340936410093 64-65 0.03295258735544383 8.882099017639848E-5 2.960699672546616E-5 2.3685597380372929E-4 0.0014581445887292084 66-67 0.035335950591843863 1.110262377204981E-4 2.960699672546616E-5 2.3685597380372929E-4 0.0015099568329987744 68-69 0.03804499079222402 1.1842798690186464E-4 2.960699672546616E-5 2.3685597380372929E-4 0.0015395638297242404 70-71 0.0411241184516725 1.1842798690186464E-4 2.960699672546616E-5 2.3685597380372929E-4 0.0015395638297242404 72-73 0.044743573801360736 1.1842798690186464E-4 2.960699672546616E-5 2.3685597380372929E-4 0.00156176907726834 74-75 0.04947329152825396 1.1842798690186464E-4 2.960699672546616E-5 2.4425772298509584E-4 0.0016061795723565394 76-77 0.05500979991591613 1.1842798690186464E-4 2.960699672546616E-5 2.516594721664624E-4 0.0016950005625329377 78-79 0.062056265136577075 1.1842798690186464E-4 2.960699672546616E-5 2.6646297052919545E-4 0.0017394110576211372 80-81 0.07048685745415356 1.3323148526459772E-4 2.960699672546616E-5 2.6646297052919545E-4 0.0017616163051652368 82-83 0.08072347657198349 1.3323148526459772E-4 2.960699672546616E-5 2.6646297052919545E-4 0.0017764198035279699 84-85 0.09394300060990413 1.3323148526459772E-4 2.960699672546616E-5 2.6646297052919545E-4 0.001835633796978902 86-87 0.11030826804990555 1.3323148526459772E-4 2.960699672546616E-5 2.960699672546616E-4 0.0018652407937043683 88-89 0.13077410453638405 1.3323148526459772E-4 2.960699672546616E-5 3.108734656173947E-4 0.001917053037973934 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 43470 0.0 94.027405 1 TCGACGG 44495 0.0 91.90406 2 TATCTCG 42075 0.0 91.523445 2 ATATCTC 42425 0.0 90.857956 1 ACGGCGC 46170 0.0 88.446434 5 GACGGCG 46550 0.0 87.78564 4 CGGCGCG 46730 0.0 87.50848 6 CGACGGC 46770 0.0 87.291466 3 ATCTCGA 44300 0.0 86.80868 3 TCTCGAG 45060 0.0 84.39591 4 CTCGAGG 46415 0.0 81.99352 5 TCGAGGG 46935 0.0 81.03451 6 CGAGGGC 47245 0.0 80.40227 7 AGGGCGC 47675 0.0 79.84644 9 GAGGGCG 48385 0.0 78.78275 8 CGCGCCG 92470 0.0 45.203693 9 GCGCGCC 94605 0.0 44.2438 8 GGCGCGC 94640 0.0 44.2174 7 CCATATT 51645 0.0 39.930458 18-19 GGGCGCG 47735 0.0 39.912838 10-11 >>END_MODULE