FastQCFastQC Report
Tue 31 May 2016
SRR1051272_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051272_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6755160
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT131540.19472521746339094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG21100.083.1076351
TCGACGG26300.066.646092
ATCTCGA39750.056.9071431
TCTCGAG49950.045.1712572
ACGGCGC41300.043.12935
GACGGCG46200.038.5552674
CGGCGCG47350.037.7189036
CTCGAGG63650.035.224213
CGACGGC51150.034.9172363
TCGAGGG66700.033.6132554
CGAGGGC69650.032.3939325
GGGCGCG70150.032.027628
AGGGCGC73450.030.7826757
CGCGCCG99800.026.27249
GAGGGCG87300.025.9534656
GCGCGCC123100.021.5697428
GGCGCGC124450.021.4120947
TATCTCG14400.017.1608121
CCATATT69800.015.99203118-19
TCCATAT66050.015.49764318-19
Produced by FastQC (version 0.11.5)