##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051272_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6755160 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2320244080081 33.0 31.0 34.0 31.0 34.0 2 32.521304010563775 34.0 31.0 34.0 31.0 34.0 3 32.64458991348835 34.0 31.0 34.0 31.0 34.0 4 36.17245868343607 37.0 35.0 37.0 35.0 37.0 5 36.081478158918515 37.0 35.0 37.0 35.0 37.0 6 36.085857033734214 37.0 35.0 37.0 35.0 37.0 7 36.05753468459666 37.0 35.0 37.0 35.0 37.0 8 36.069199249166566 37.0 35.0 37.0 35.0 37.0 9 37.84855355017498 39.0 38.0 39.0 35.0 39.0 10-11 37.81743815098384 39.0 38.0 39.0 35.0 39.0 12-13 37.794443876977006 39.0 38.0 39.0 35.0 39.0 14-15 39.16367621196241 40.5 39.0 41.0 36.0 41.0 16-17 39.12778653651431 40.0 38.5 41.0 36.0 41.0 18-19 39.08465417547475 40.0 38.5 41.0 36.0 41.0 20-21 39.045897876586196 40.0 38.5 41.0 35.5 41.0 22-23 38.947687767573235 40.0 38.0 41.0 35.0 41.0 24-25 38.849578248331646 40.0 38.0 41.0 35.0 41.0 26-27 38.671281065141315 40.0 38.0 41.0 34.5 41.0 28-29 38.465797775330266 40.0 38.0 41.0 34.0 41.0 30-31 38.295061330893716 40.0 38.0 41.0 34.0 41.0 32-33 38.46556358398617 40.0 38.0 41.0 34.0 41.0 34-35 38.42594675773779 40.0 38.0 41.0 34.0 41.0 36-37 38.343603038270004 40.0 38.0 41.0 34.0 41.0 38-39 38.2218119185926 40.0 38.0 41.0 33.0 41.0 40-41 38.060830313419665 40.0 37.5 41.0 33.0 41.0 42-43 37.926558882395085 40.0 37.0 41.0 33.0 41.0 44-45 37.81337362845588 40.0 37.0 41.0 33.0 41.0 46-47 37.641959849951746 40.0 37.0 41.0 32.5 41.0 48-49 37.51139884177429 40.0 36.0 41.0 32.0 41.0 50-51 37.52295489670119 39.0 36.0 41.0 32.0 41.0 52-53 37.3451636970849 39.0 36.0 41.0 32.0 41.0 54-55 37.00128701614766 39.0 35.0 41.0 31.0 41.0 56-57 36.74444972435886 39.0 35.0 40.5 31.0 41.0 58-59 36.45144630179004 38.0 35.0 40.0 31.0 41.0 60-61 36.13482160304123 37.5 35.0 40.0 31.0 41.0 62-63 35.7480036742283 37.0 35.0 40.0 30.0 41.0 64-65 35.33674857442311 36.5 34.0 39.0 30.0 41.0 66-67 34.91068427690831 36.0 34.0 39.0 29.0 41.0 68-69 34.50832304490197 35.0 34.0 38.5 29.0 40.0 70-71 34.07753954014413 35.0 34.0 37.0 29.0 39.5 72-73 33.68753197555647 35.0 33.0 37.0 28.0 39.0 74-75 33.342109439302696 35.0 33.0 36.0 28.0 39.0 76-77 32.784817606096674 35.0 32.5 36.0 27.0 37.5 78-79 32.70571984971488 35.0 33.0 35.0 27.0 37.0 80-81 32.48417661461757 35.0 33.0 35.0 26.5 37.0 82-83 32.21205315640192 35.0 32.5 35.0 26.0 36.0 84-85 31.994127970321948 35.0 32.0 35.0 26.0 36.0 86-87 31.706251591376073 35.0 32.0 35.0 25.0 35.5 88-89 31.536511274344353 34.0 32.0 35.0 25.0 35.0 90-91 31.299954479242537 34.0 32.0 35.0 24.5 35.0 92-93 31.019254466215457 34.0 31.5 35.0 23.5 35.0 94-95 30.72733347544692 34.0 31.0 35.0 23.0 35.0 96-97 30.464041710336986 34.0 31.0 35.0 20.0 35.0 98-99 30.05983707269702 34.0 31.0 35.0 16.5 35.0 100-101 28.28191293766543 32.5 28.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.42503781112156247 1101 2 0.4124071575644521 1101 3 0.3538430580336396 1101 4 0.2349648924364729 1101 5 0.23921571344844494 1101 6 0.24997968363854284 1101 7 0.2816238278779224 1101 8 0.23467844588281395 1101 9 0.32063260799822757 1101 10-11 0.3295021402890512 1101 12-13 0.3569191510789338 1101 14-15 0.443132310166483 1101 16-17 0.37834336133928304 1101 18-19 0.36113366647905565 1101 20-21 0.3734703927845189 1101 22-23 0.37882601991927345 1101 24-25 0.4011587102214236 1101 26-27 0.41352312090865695 1101 28-29 0.42311248865896545 1101 30-31 0.41594029911927777 1101 32-33 0.38175436406436347 1101 34-35 0.4086876674582314 1101 36-37 0.3735951885740789 1101 38-39 0.37501481737032094 1101 40-41 0.38498034277505866 1101 42-43 0.3910047777619141 1101 44-45 0.3306730764605774 1101 46-47 0.3248446103334217 1101 48-49 0.3756633731308483 1101 50-51 0.44768541240846815 1101 52-53 0.44692917032212165 1101 54-55 0.515215656825518 1101 56-57 0.40224794824376886 1101 58-59 0.37060451641519165 1101 60-61 0.2877123487367612 1101 62-63 0.38438539174615727 1101 64-65 0.3612699708488307 1101 66-67 0.28528949242625856 1101 68-69 0.26744560331212597 1101 70-71 0.2814475158459899 1101 72-73 0.17187047089900176 1101 74-75 0.22187195330626963 1101 76-77 0.2091854740744239 1101 78-79 0.21437534178133433 1101 80-81 0.15500656256269707 1101 82-83 -0.02457201455342073 1101 84-85 0.05028390082068768 1101 86-87 0.28293074286467856 1101 88-89 0.24902836013371754 1101 90-91 0.1732686675200803 1101 92-93 0.07919763976773453 1101 94-95 0.08353772633069312 1101 96-97 0.10903892469179866 1101 98-99 0.21429524881700956 1101 100-101 0.2630344114990564 1102 1 0.3746483883118614 1102 2 0.35176346996546926 1102 3 0.29467960685816763 1102 4 0.19342618046636773 1102 5 0.22311421632526418 1102 6 0.23109930689062708 1102 7 0.23854461701478158 1102 8 0.22167585420611857 1102 9 0.2451800414700429 1102 10-11 0.2552713361341219 1102 12-13 0.2525349026967021 1102 14-15 0.34504338543582946 1102 16-17 0.3582439650840499 1102 18-19 0.34037039748691456 1102 20-21 0.3531557636832545 1102 22-23 0.3089973358552953 1102 24-25 0.32361268612401517 1102 26-27 0.3602381552424916 1102 28-29 0.4450609236840961 1102 30-31 0.3837389412946379 1102 32-33 0.3794679853914289 1102 34-35 0.40443940820028246 1102 36-37 0.4249802841206858 1102 38-39 0.43461662811309765 1102 40-41 0.3983123758969498 1102 42-43 0.3996301861831739 1102 44-45 0.37495550978272973 1102 46-47 0.33700069469163196 1102 48-49 0.41127394596975364 1102 50-51 0.5223191048303661 1102 52-53 0.5141536937727125 1102 54-55 0.5768490758348221 1102 56-57 0.454960178705349 1102 58-59 0.4489433217505976 1102 60-61 0.4212986976621451 1102 62-63 0.3712638836585782 1102 64-65 0.39604677752396356 1102 66-67 0.3824383424677791 1102 68-69 0.37311124118401295 1102 70-71 0.4351431291454517 1102 72-73 0.44651454654068345 1102 74-75 0.40279044607236614 1102 76-77 0.4019793397889302 1102 78-79 0.37174881909324853 1102 80-81 0.465654209496428 1102 82-83 0.3970896697979711 1102 84-85 0.38366836613418087 1102 86-87 0.5420315147493113 1102 88-89 0.5765558427672453 1102 90-91 0.4937834322373149 1102 92-93 0.34811623533086333 1102 94-95 0.4233508929637324 1102 96-97 0.4056720762639294 1102 98-99 0.4686149171178009 1102 100-101 0.5909682164057379 1103 1 0.2890383955904312 1103 2 0.2748530602764845 1103 3 0.22366436438207415 1103 4 0.15206232756641214 1103 5 0.17603341848727183 1103 6 0.1383090920708625 1103 7 0.16425265577912285 1103 8 0.15840966649953714 1103 9 0.16758745617080706 1103 10-11 0.1356551825218375 1103 12-13 0.19192452044941177 1103 14-15 0.29064418886754595 1103 16-17 0.2618067349385953 1103 18-19 0.21465635003071526 1103 20-21 0.2241533542784495 1103 22-23 0.20909173568634998 1103 24-25 0.191930043559708 1103 26-27 0.20816348488470737 1103 28-29 0.23043634177707162 1103 30-31 0.21178250498061146 1103 32-33 0.14809583581785546 1103 34-35 0.23123535634096726 1103 36-37 0.2750082953452093 1103 38-39 0.2339086381814468 1103 40-41 0.17810759761166395 1103 42-43 0.27291261280252144 1103 44-45 0.2285340747404021 1103 46-47 0.2769007758583726 1103 48-49 0.36313332833937295 1103 50-51 0.3294898403274189 1103 52-53 0.3049567969469109 1103 54-55 0.3971215419032603 1103 56-57 0.36016135904365143 1103 58-59 0.27951323316991505 1103 60-61 0.26242503234467307 1103 62-63 0.3364441985844451 1103 64-65 0.3017491004790074 1103 66-67 0.31455033678429345 1103 68-69 0.2647281586985102 1103 70-71 0.3222132947445928 1103 72-73 0.37523603057923083 1103 74-75 0.3934231243027 1103 76-77 0.29770917621578263 1103 78-79 0.37422452562615405 1103 80-81 0.4303385035595184 1103 82-83 0.3071757173088585 1103 84-85 0.3703266910421732 1103 86-87 0.5329045690449448 1103 88-89 0.48042619893196914 1103 90-91 0.32249154350116527 1103 92-93 0.26814002434494455 1103 94-95 0.3738944473374275 1103 96-97 0.5061584702734017 1103 98-99 0.5386627641799997 1103 100-101 0.7078611044721796 1104 1 0.37303365202165395 1104 2 0.3646633325288491 1104 3 0.27565517112611104 1104 4 0.17784338924453635 1104 5 0.2154376463266061 1104 6 0.19454458723723178 1104 7 0.21510339839841208 1104 8 0.2046433903746987 1104 9 0.2515433632042061 1104 10-11 0.2543952013913682 1104 12-13 0.2743490744009094 1104 14-15 0.33612977975910496 1104 16-17 0.26341487163026756 1104 18-19 0.332041525487206 1104 20-21 0.3158745444497484 1104 22-23 0.35880277675912 1104 24-25 0.29707272002072926 1104 26-27 0.35883340895105675 1104 28-29 0.3726115185220209 1104 30-31 0.3901853492942067 1104 32-33 0.36087481190970294 1104 34-35 0.39514393180002116 1104 36-37 0.3846567733759727 1104 38-39 0.36098871191007476 1104 40-41 0.38410621326757166 1104 42-43 0.4051717753927804 1104 44-45 0.31217069698627853 1104 46-47 0.3367549323505017 1104 48-49 0.37796016893871354 1104 50-51 0.39796935754508667 1104 52-53 0.40173267982601146 1104 54-55 0.3311404841259815 1104 56-57 0.2301713905258964 1104 58-59 0.2568000435427322 1104 60-61 0.18896189456958723 1104 62-63 0.22410390121847712 1104 64-65 0.27497361404907394 1104 66-67 0.1442010821223505 1104 68-69 0.17183009532539018 1104 70-71 0.32160602169377484 1104 72-73 0.29545715532732686 1104 74-75 0.2699810569821821 1104 76-77 0.16306976150386276 1104 78-79 0.2213012120331328 1104 80-81 0.30725817336647054 1104 82-83 0.13829459186244009 1104 84-85 0.15978694233092483 1104 86-87 0.36250390556544687 1104 88-89 0.4057509530812027 1104 90-91 0.34630842008736096 1104 92-93 0.24260806185885286 1104 94-95 0.3764721966100666 1104 96-97 0.33835922594550993 1104 98-99 0.4938266088439427 1104 100-101 0.6418113443739344 1105 1 -0.10863970299720904 1105 2 0.06709107663568403 1105 3 0.048165174762743845 1105 4 0.03644619687898398 1105 5 0.012590715081884696 1105 6 0.03318053897972817 1105 7 0.05970218786231385 1105 8 -0.02289529876660623 1105 9 -0.008540963730766293 1105 10-11 -0.025455612563256125 1105 12-13 0.02718013276996345 1105 14-15 0.007665196258699325 1105 16-17 0.024871666267344494 1105 18-19 -0.037926438606568524 1105 20-21 -0.009806806709519833 1105 22-23 0.07662603396525469 1105 24-25 0.011287851668292603 1105 26-27 0.056228116261749506 1105 28-29 0.020653232022425527 1105 30-31 0.0625562251067251 1105 32-33 0.11291318095197056 1105 34-35 0.11108006784246527 1105 36-37 0.149066838134992 1105 38-39 0.13965276451371267 1105 40-41 0.15800111318250032 1105 42-43 0.14465757656222422 1105 44-45 0.07630746204984717 1105 46-47 -0.08524201821656163 1105 48-49 0.1708130689955425 1105 50-51 0.1273159118154581 1105 52-53 0.11997200799734742 1105 54-55 0.0956083838119639 1105 56-57 0.012877614397766024 1105 58-59 0.09469455771263569 1105 60-61 0.08804929553669183 1105 62-63 0.16374477118950637 1105 64-65 0.19434023626522645 1105 66-67 0.19739255062761174 1105 68-69 0.15279522271143975 1105 70-71 0.10420451060179658 1105 72-73 0.15770457456881815 1105 74-75 0.21604462715274764 1105 76-77 0.2030088492015878 1105 78-79 0.2896219569230567 1105 80-81 0.3931133072857378 1105 82-83 0.30840943087455486 1105 84-85 0.20314265026571832 1105 86-87 0.367170704363339 1105 88-89 0.3423599211620605 1105 90-91 0.38064003241285604 1105 92-93 0.2777167001000542 1105 94-95 0.34969874248865196 1105 96-97 0.46990897209300186 1105 98-99 0.6325786761975181 1105 100-101 0.8413204109712495 1106 1 -0.16696006192007218 1106 2 -0.049065142639932446 1106 3 -0.046546668408211644 1106 4 -0.030735845312051424 1106 5 -0.36526462028908213 1106 6 -0.2497304261767468 1106 7 -0.13911993275760182 1106 8 -0.3368990246123573 1106 9 -0.5021582937366702 1106 10-11 -0.11134196574928978 1106 12-13 -0.0862332801706387 1106 14-15 -0.27082065260779586 1106 16-17 -0.09419109334123021 1106 18-19 -0.20825106665828486 1106 20-21 -0.17566614347143172 1106 22-23 -0.14427461835443012 1106 24-25 -0.10263700149007349 1106 26-27 -0.08685206499639975 1106 28-29 -0.1509868827537204 1106 30-31 -0.24156490620669757 1106 32-33 -0.1638787446195309 1106 34-35 -0.11119078076821154 1106 36-37 -0.09454996054163445 1106 38-39 -0.08363035529638552 1106 40-41 -0.01728721688157009 1106 42-43 -0.1511908987881796 1106 44-45 -0.14285049094670654 1106 46-47 -0.2099293710546135 1106 48-49 -0.05442545317724523 1106 50-51 -0.026277174481073473 1106 52-53 -0.1255084183784021 1106 54-55 -0.07686603070958142 1106 56-57 -0.14500250112995872 1106 58-59 -0.08089060975637352 1106 60-61 -0.16836555783108764 1106 62-63 -0.10925073075462421 1106 64-65 -0.2022567029102973 1106 66-67 -0.2127791989388328 1106 68-69 -0.1837477385271029 1106 70-71 -0.10407959561569413 1106 72-73 -0.017752286637580994 1106 74-75 -0.09836041061569745 1106 76-77 -0.1858188999520749 1106 78-79 -0.1011566902221972 1106 80-81 -0.06486020118274638 1106 82-83 -0.1613777938250891 1106 84-85 -0.1415671304801407 1106 86-87 0.07580014425275294 1106 88-89 0.0516712798960377 1106 90-91 -0.051060088827377115 1106 92-93 -0.1776155227941132 1106 94-95 -0.03629350831015188 1106 96-97 -0.010040660319955208 1106 98-99 0.12452084859120305 1106 100-101 0.1753724051112009 1107 1 -0.2071296291342577 1107 2 -0.14754413456704896 1107 3 -0.18245451723573325 1107 4 -0.12238516424653767 1107 5 -0.14898311674107845 1107 6 -0.16907830138939772 1107 7 -0.12403733916232085 1107 8 -0.14975752612629378 1107 9 -0.2511302687956274 1107 10-11 -0.2589406728896151 1107 12-13 -0.29471520650194094 1107 14-15 -0.2677852224602617 1107 16-17 -0.30817031939532313 1107 18-19 -0.27291514864348443 1107 20-21 -0.2698785133846613 1107 22-23 -0.34285376456585936 1107 24-25 -0.3974318544009279 1107 26-27 -0.44918373507820775 1107 28-29 -0.4264847070366855 1107 30-31 -0.5006343835736615 1107 32-33 -0.5088203542090355 1107 34-35 -0.43161569507341824 1107 36-37 -0.3487207729016788 1107 38-39 -0.2743055383714079 1107 40-41 -0.26254752517348834 1107 42-43 -0.30160992694714395 1107 44-45 -0.40784327139888177 1107 46-47 -0.3930091332924235 1107 48-49 -0.2576658372461651 1107 50-51 -0.17713922700551166 1107 52-53 -0.2408829828586434 1107 54-55 -0.3369829843113763 1107 56-57 -0.4729379464857928 1107 58-59 -0.3367832278053413 1107 60-61 -0.36300412361685375 1107 62-63 -0.30753498375361943 1107 64-65 -0.30908119367411047 1107 66-67 -0.3330836963733077 1107 68-69 -0.3403678164305646 1107 70-71 -0.3035609028177291 1107 72-73 -0.2991773598652614 1107 74-75 -0.2578738019601019 1107 76-77 -0.36972705952201323 1107 78-79 -0.37003326659456803 1107 80-81 -0.27921559319796785 1107 82-83 -0.30118812161971675 1107 84-85 -0.4366828594904213 1107 86-87 -0.19850662233790572 1107 88-89 -0.22990884169598047 1107 90-91 -0.44615681912198113 1107 92-93 -0.4834606117696012 1107 94-95 -0.4709239066450266 1107 96-97 -0.5465651561141378 1107 98-99 -0.5024820502643799 1107 100-101 -0.3333840669503374 1108 1 -0.36730164055779824 1108 2 -0.27710808749795035 1108 3 -0.281229952486413 1108 4 -0.1615957163135633 1108 5 -0.3500402406837111 1108 6 -0.4994833195590971 1108 7 -0.7145886180090102 1108 8 -0.48081642898976185 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2305 12-13 0.052636969859754856 2305 14-15 0.13072456112510622 2305 16-17 0.10290524944523582 2305 18-19 0.12176015603370871 2305 20-21 0.13914572195560737 2305 22-23 0.15243892942821446 2305 24-25 0.1038004022367005 2305 26-27 0.16842737712973843 2305 28-29 0.10463628096147914 2305 30-31 0.16666728019242072 2305 32-33 0.17047994701927394 2305 34-35 0.16276367119563417 2305 36-37 0.17687658605587586 2305 38-39 0.1549992917161731 2305 40-41 0.2174873165572393 2305 42-43 0.28386505092721137 2305 44-45 0.303164730556567 2305 46-47 0.34644040403279774 2305 48-49 0.31199366459355815 2305 50-51 0.23292214130316324 2305 52-53 0.3044440028923958 2305 54-55 0.25852202674053615 2305 56-57 0.28276963914365183 2305 58-59 0.3473837231302781 2305 60-61 0.44060574762646354 2305 62-63 0.4021650708865536 2305 64-65 0.37818429103902673 2305 66-67 0.5255033612968205 2305 68-69 0.5038144685758894 2305 70-71 0.48784744070741226 2305 72-73 0.5075552687239053 2305 74-75 0.4830011646772334 2305 76-77 0.4432949118549274 2305 78-79 0.5142324473461599 2305 80-81 0.6070031742576063 2305 82-83 0.6271464460092275 2305 84-85 0.6289089832769434 2305 86-87 0.7604075465451956 2305 88-89 0.737379324353828 2305 90-91 0.7133084905990259 2305 92-93 0.8983388798508827 2305 94-95 0.9549439195714982 2305 96-97 0.9870452219918953 2305 98-99 1.0617158848432595 2305 100-101 1.2058975911264973 2306 1 -0.5576048656541097 2306 2 -0.9879644039342423 2306 3 -0.785191500362167 2306 4 -0.37843562293574706 2306 5 -0.2488511503230768 2306 6 -0.23064865722789563 2306 7 -0.09558692562151094 2306 8 -0.08585547554434925 2306 9 -0.0855786142640298 2306 10-11 -0.37267938184600524 2306 12-13 -0.2962167554002164 2306 14-15 -0.17844144599142453 2306 16-17 -0.24074641102682648 2306 18-19 -0.17970618976119823 2306 20-21 -0.15345480406170964 2306 22-23 -0.09040918841776602 2306 24-25 -0.11410187623067714 2306 26-27 -0.15223377810345795 2306 28-29 -0.15619138129056864 2306 30-31 -0.09139360099663207 2306 32-33 -0.17964373953171275 2306 34-35 -0.2437493403798925 2306 36-37 -0.27247297709730844 2306 38-39 -0.15314801276975487 2306 40-41 -0.2590626674144687 2306 42-43 -0.21615091612247284 2306 44-45 -0.23118420402467166 2306 46-47 -0.20516511513613267 2306 48-49 -0.1216798185824075 2306 50-51 -0.10510441262469783 2306 52-53 -0.07948046966710365 2306 54-55 -0.21252805471523573 2306 56-57 -0.21004049316402984 2306 58-59 -0.21380017882508895 2306 60-61 -0.24969127604936858 2306 62-63 -0.12698109898131094 2306 64-65 -0.13453982898356998 2306 66-67 -0.09903697237476194 2306 68-69 -0.08204366765325233 2306 70-71 -0.07479089178394105 2306 72-73 -0.07100208592957102 2306 74-75 -0.06681130584812678 2306 76-77 -0.06550667517939956 2306 78-79 0.01848109699550804 2306 80-81 0.1437000574195153 2306 82-83 0.07910186569542788 2306 84-85 0.04017296253830693 2306 86-87 0.1456877455682246 2306 88-89 0.03313909033608198 2306 90-91 0.13560855922282755 2306 92-93 0.18850048641935402 2306 94-95 0.2658911057622504 2306 96-97 0.1315369310636747 2306 98-99 0.30102115496316273 2306 100-101 0.4940366852922935 2307 1 -1.6323031022487484 2307 2 -2.1572648334280586 2307 3 -1.7240638275759785 2307 4 -0.882941436312386 2307 5 -0.4971187533522681 2307 6 -0.41426183370121095 2307 7 -0.32104558807620265 2307 8 -0.4647545689055761 2307 9 -0.4028040088488325 2307 10-11 -0.324046122397597 2307 12-13 -0.3387429051972859 2307 14-15 -0.6360983949165089 2307 16-17 -0.5052357786651598 2307 18-19 -0.3452593621764777 2307 20-21 -0.4009448214818292 2307 22-23 -0.4214253520221831 2307 24-25 -0.40859898568857034 2307 26-27 -0.3657900625874646 2307 28-29 -0.3211231356944495 2307 30-31 -0.7028974303595987 2307 32-33 -0.45035924678551 2307 34-35 -0.41233685811647547 2307 36-37 -0.29872354452849237 2307 38-39 -0.3342767273851379 2307 40-41 -0.2617765977581712 2307 42-43 -0.25606180591240957 2307 44-45 -0.239454159267126 2307 46-47 -0.30491848957088763 2307 48-49 -0.317193686772292 2307 50-51 -0.29149542802783657 2307 52-53 -0.2969955805061417 2307 54-55 -0.14994809000893383 2307 56-57 -0.1327027127232867 2307 58-59 -0.25269437751116897 2307 60-61 -0.2286864049194932 2307 62-63 -0.1218340863642382 2307 64-65 -0.09760352260121152 2307 66-67 -0.17630053093902376 2307 68-69 -0.037188307056752024 2307 70-71 -0.1470136316975399 2307 72-73 -0.1592438573253716 2307 74-75 -0.17638341321534057 2307 76-77 -0.11053410933259755 2307 78-79 -0.18553644837624717 2307 80-81 -0.1492748361876366 2307 82-83 -0.08243720326414206 2307 84-85 -0.13995078911531067 2307 86-87 -0.06962634982727423 2307 88-89 -0.09055338421700654 2307 90-91 -0.138382507777024 2307 92-93 -0.07516906806946011 2307 94-95 0.02081372200357734 2307 96-97 0.01439370733057288 2307 98-99 0.051380051238563595 2307 100-101 0.29176456420547225 2308 1 -1.2514654314369338 2308 2 -1.1794776939226281 2308 3 -0.7694009539762838 2308 4 -0.5217122529630274 2308 5 -0.6557050504725552 2308 6 -0.5937343570807698 2308 7 -1.518712185811836 2308 8 -0.5744417860196975 2308 9 -0.5446936609349251 2308 10-11 -0.8013787628978051 2308 12-13 -0.9123804828209643 2308 14-15 -0.6375470287461411 2308 16-17 -0.8687181413434999 2308 18-19 -0.6096647657464445 2308 20-21 -0.670550301866129 2308 22-23 -0.517214627387439 2308 24-25 -0.4939972025329382 2308 26-27 -0.4995091009155246 2308 28-29 -1.3166827850093412 2308 30-31 -0.6352947587601179 2308 32-33 -0.5006174783186736 2308 34-35 -1.143701781842985 2308 36-37 -0.6584244282044693 2308 38-39 -0.49247260842157914 2308 40-41 -0.9857740583241181 2308 42-43 -0.4222027307356413 2308 44-45 -0.5100720602921527 2308 46-47 -0.45588343400972775 2308 48-49 -0.3407773968493544 2308 50-51 -0.4216051686510056 2308 52-53 -0.5179267860495145 2308 54-55 -0.9894488088309785 2308 56-57 -1.7295608870454942 2308 58-59 -1.097634735592301 2308 60-61 -0.6804886954752405 2308 62-63 -0.41999060492140927 2308 64-65 -0.27197604142495635 2308 66-67 -0.24849348127361282 2308 68-69 -0.18484818579780438 2308 70-71 -0.0883859802998046 2308 72-73 -0.20618466459097817 2308 74-75 -0.12582501270697577 2308 76-77 -1.1080550993030585 2308 78-79 -0.5962969325325176 2308 80-81 -0.09223380526669445 2308 82-83 0.029392383217931695 2308 84-85 0.131821895104693 2308 86-87 -0.2215612464461003 2308 88-89 -0.20719300948709574 2308 90-91 -0.78233780528317 2308 92-93 0.1039374174176757 2308 94-95 0.26353696222798106 2308 96-97 0.09363950744619487 2308 98-99 -0.3878783648735755 2308 100-101 0.36675065994538514 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 15.0 12 140.0 13 799.0 14 2595.0 15 5707.0 16 9248.0 17 13370.0 18 20233.0 19 30231.0 20 28218.0 21 27486.0 22 28798.0 23 31402.0 24 36887.0 25 44511.0 26 54791.0 27 69590.0 28 86393.0 29 107000.0 30 133711.0 31 166589.0 32 210852.0 33 275610.0 34 385741.0 35 591809.0 36 1038413.0 37 1801142.0 38 1392312.0 39 161487.0 40 79.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.984756707556645 27.991926362789116 24.84014817366465 23.183168755989584 2 26.402555937028875 27.479550253615937 26.625128643619572 19.49276516573562 3 25.213796235670372 26.331758583491965 25.603499080034826 22.85094610080284 4 26.624427288809276 25.363182164718772 24.568025876553445 23.444364669918507 5 26.39768011347773 25.055490930041902 23.930617166911567 24.6162117895688 6 26.03969410050983 24.446941301168295 23.503662385494938 26.009702212826934 7 26.487559139975957 24.757873980779138 23.90957431060108 24.84499256864382 8 25.759730339473823 24.312407108047772 24.315826716169564 25.612035836308838 9 25.180558269530255 23.703672451873828 24.82857548895955 26.287193789636365 10-11 25.45523718165077 24.420480047844904 24.48733264645101 25.636950124053314 12-13 25.40554923939625 24.56597623150303 24.578240929896552 25.450233599204164 14-15 25.2297576963388 24.68481131460987 24.726113075041894 25.359317914009438 16-17 25.342937843071073 24.788842899353973 24.65763209161589 25.21058716595906 18-19 25.003723079838224 24.686239852201872 24.89012103340261 25.41991603455729 20-21 24.830070642294185 24.66157722392956 25.072522338479033 25.435829795297227 22-23 24.936167315059894 24.73301890702811 25.284597256023545 25.046216521888454 24-25 24.91800155392357 24.464525887882573 25.648465539335607 24.969007018858257 26-27 24.827746493051237 24.50368311039265 25.74241764813861 24.926152748417508 28-29 24.933998602549753 24.595864494697388 25.658008100474305 24.812128802278554 30-31 24.887782080661303 24.458177156425606 25.727947228489036 24.926093534424055 32-33 24.856324646640495 24.52377885942006 25.78154329431131 24.838353199628134 34-35 24.93233320898395 24.643509554177843 25.65875567714162 24.76540155969659 36-37 24.91973543187726 24.549004020630154 25.589038601602333 24.942221945890253 38-39 24.88582801887742 24.602659300445882 25.59554473913275 24.915967941543947 40-41 25.03049520662723 24.724447681476086 25.370687000752017 24.874370111144668 42-43 24.996832051350378 24.700480817626822 25.304382131585335 24.99830499943747 44-45 24.914391368968314 24.813979239573897 25.37309997098514 24.898529420472645 46-47 25.030894901083023 24.905679510181848 25.172312720942212 24.891112867792916 48-49 24.991880281148042 24.855177375517382 25.199950852385435 24.95299149094914 50-51 24.89791507529059 24.884473498777233 25.3142486632441 24.90336276268808 52-53 24.980488989157916 24.982739120909052 25.182290278838693 24.854481611094332 54-55 24.975774074929387 24.947462384310658 25.141713889826445 24.935049650933507 56-57 24.917833182337652 25.01185760218855 25.26456812273877 24.805741092735033 58-59 24.977424664996832 25.085334766311973 25.159633524594533 24.777607044096662 60-61 24.983405278335375 24.9498309440487 25.117310322775477 24.949453454840448 62-63 24.946618584903984 25.048829339349478 25.143068409926634 24.861483665819904 64-65 24.97412348486194 25.159640926343712 25.05809632932455 24.808139259469797 66-67 24.99616589392405 25.021968391570297 25.058644058763967 24.923221655741685 68-69 24.958927693792596 25.13194358090704 25.066919214348736 24.84220951095163 70-71 25.050087636710305 25.249823838369483 24.941133888760593 24.758954636159615 72-73 25.017283084338494 25.16499239100184 24.941489172721298 24.87623535193837 74-75 24.914028683258426 25.277846860770136 25.00410056904648 24.80402388692496 76-77 24.9868915021998 25.39588255496539 24.87351890998881 24.743707032846 78-79 24.963198503070245 25.29680274042362 24.902696605261756 24.83730215124438 80-81 24.882289982768725 25.354588196282545 24.956411099070934 24.806710721877796 82-83 24.929727793272104 25.483430444282597 24.850839950497104 24.7360018119482 84-85 24.938965176250452 25.38625287928043 24.819648979446825 24.855132965022296 86-87 24.890513326109225 25.496894226043498 24.90472468453745 24.707867763309828 88-89 24.976721498824602 25.582946962026064 24.77592684703249 24.66440469211684 90-91 24.966062980003436 25.504976936149546 24.733796090692152 24.795163993154862 92-93 24.873135860078506 25.602126789160195 24.77717957658883 24.747557774172467 94-95 25.010319889559973 25.719755395857046 24.657737541208316 24.612187173374664 96-97 24.897554232435223 25.696713748949968 24.6386632321561 24.767068786458708 98-99 24.861180194103472 25.743076403815753 24.703811604758435 24.691931797322344 100-101 24.97478223307286 25.88496079588368 24.3192683279947 24.820988643048764 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3946.0 1 2301.0 2 570.5 3 426.0 4 373.5 5 393.5 6 416.5 7 423.5 8 411.5 9 435.0 10 455.0 11 473.0 12 542.0 13 646.5 14 824.0 15 1071.0 16 2251.5 17 2657.5 18 2068.0 19 2261.0 20 2894.0 21 3622.0 22 4367.0 23 5402.0 24 6688.5 25 8335.0 26 10600.5 27 13592.5 28 16517.0 29 20348.5 30 25422.5 31 29869.5 32 34914.0 33 43056.5 34 51346.0 35 60402.0 36 74377.0 37 87512.5 38 101460.5 39 118744.5 40 135536.5 41 149293.0 42 164459.0 43 182026.5 44 201518.5 45 223784.0 46 243479.5 47 266740.5 48 287689.5 49 299036.5 50 303792.5 51 307064.5 52 314141.0 53 321661.5 54 322436.5 55 315787.5 56 299650.5 57 274406.0 58 250006.5 59 227127.5 60 200254.5 61 169956.0 62 139353.0 63 107358.5 64 78285.5 65 56510.0 66 40187.0 67 29131.5 68 21758.0 69 16821.5 70 13766.5 71 10987.0 72 9372.5 73 7787.0 74 5914.5 75 5135.5 76 4370.5 77 2962.0 78 1805.5 79 1111.0 80 682.5 81 473.5 82 371.0 83 306.5 84 219.0 85 134.0 86 54.5 87 3.5 88 2.5 89 1.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04164224089436815 2 9.474238952149173E-4 3 1.9244547871553004E-4 4 1.480349836273308E-4 5 2.9606996725466164E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.2205247544099624E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 7.401749181366541E-6 94-95 7.401749181366541E-6 96-97 7.401749181366541E-6 98-99 0.0 100-101 2.9606996725466164E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6755160.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.060595181117435 #Duplication Level Percentage of deduplicated Percentage of total 1 82.26636249182138 43.65102157201598 2 9.076730395206408 9.632334341363824 3 2.7000080260845714 4.297920985735241 4 1.2359083323222313 2.623121268092795 5 0.7512107996590507 1.99298460681962 6 0.5255557149001995 1.673177942006535 7 0.40002591299887946 1.4857929122113311 8 0.3337596295058202 1.416758767120645 9 0.26709815494716227 1.2755148365947282 >10 2.2419966410293317 22.51050899162784 >50 0.15646977220312208 5.709258873649242 >100 0.04448311804571807 3.190443492515637 >500 8.37896393484521E-5 0.033611069572794056 >1k 2.792923973579251E-4 0.31261568092474956 >5k 0.0 0.0 >10k+ 2.792923946455551E-5 0.19493465974906068 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13154 0.19472521746339094 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.480349836273308E-5 0.0 0.0 0.0 2.960699672546616E-5 2 1.480349836273308E-5 0.0 0.0 0.0 2.960699672546616E-5 3 1.480349836273308E-5 0.0 0.0 0.0 2.960699672546616E-5 4 1.480349836273308E-5 0.0 0.0 0.0 2.960699672546616E-5 5 1.480349836273308E-5 0.0 0.0 0.0 7.40174918136654E-5 6 2.960699672546616E-5 0.0 0.0 1.480349836273308E-5 7.40174918136654E-5 7 2.960699672546616E-5 0.0 0.0 1.480349836273308E-5 8.882099017639848E-5 8 4.441049508819924E-5 2.960699672546616E-5 0.0 2.960699672546616E-5 1.9244547871553004E-4 9 4.441049508819924E-5 2.960699672546616E-5 0.0 2.960699672546616E-5 2.0724897707826312E-4 10-11 6.661574263229886E-5 2.960699672546616E-5 0.0 2.960699672546616E-5 2.8866821807329505E-4 12-13 9.622273935776502E-5 2.960699672546616E-5 0.0 3.70087459068327E-5 3.478822115242274E-4 14-15 1.3323148526459772E-4 2.960699672546616E-5 0.0 4.441049508819924E-5 3.848909574310601E-4 16-17 2.6646297052919545E-4 2.960699672546616E-5 0.0 5.921399345093232E-5 4.070962049751597E-4 18-19 4.885154459701917E-4 2.960699672546616E-5 0.0 5.921399345093232E-5 4.367032017006259E-4 20-21 8.21594159131686E-4 2.960699672546616E-5 0.0 5.921399345093232E-5 4.66310198426092E-4 22-23 0.0013989305952782762 2.960699672546616E-5 0.0 7.40174918136654E-5 5.403276902397574E-4 24-25 0.0023833632364000257 2.960699672546616E-5 0.0 7.40174918136654E-5 5.995416836906898E-4 26-27 0.0035380361086932064 2.960699672546616E-5 0.0 7.40174918136654E-5 6.217469312347894E-4 28-29 0.004603887990809989 3.70087459068327E-5 0.0 7.40174918136654E-5 6.735591755043552E-4 30-31 0.00564753462538267 4.441049508819924E-5 0.0 7.40174918136654E-5 7.179696705925544E-4 32-33 0.006972447728847281 5.181224426956578E-5 0.0 7.40174918136654E-5 7.475766673180206E-4 34-35 0.00831956607985599 7.40174918136654E-5 0.0 7.40174918136654E-5 7.697819148621202E-4 36-37 0.00954085469478147 7.40174918136654E-5 0.0 7.40174918136654E-5 8.141924099503195E-4 38-39 0.010754741560525582 7.40174918136654E-5 0.0 7.40174918136654E-5 8.660046542198853E-4 40-41 0.011753977700010066 8.141924099503194E-5 0.0 8.141924099503194E-5 9.32620396852184E-4 42-43 0.012827231331308215 8.882099017639848E-5 0.0 9.622273935776502E-5 0.0010066378886658496 44-45 0.014137340936410092 9.622273935776502E-5 0.0 1.0362448853913156E-4 0.001095458878842248 46-47 0.015299415557884638 1.0362448853913156E-4 0.0 1.0362448853913156E-4 0.00117687811983728 48-49 0.01621723245637409 1.0362448853913156E-4 0.0 1.0362448853913156E-4 0.0012434938624695789 50-51 0.016898193381059814 1.0362448853913156E-4 0.0 1.0362448853913156E-4 0.0013175113542832442 52-53 0.018015857507446158 1.0362448853913156E-4 0.0 1.0362448853913156E-4 0.001339716601827344 54-55 0.019562823086351766 1.0362448853913156E-4 0.0 1.1842798690186464E-4 0.0013915288460969095 56-57 0.021057976420987806 1.0362448853913156E-4 0.0 1.1842798690186464E-4 0.0014433410903664755 58-59 0.02246430876544745 1.0362448853913156E-4 0.0 1.1842798690186464E-4 0.0015025550838174077 60-61 0.02381142711645616 1.0362448853913156E-4 0.0 1.1842798690186464E-4 0.0015691708264497066 62-63 0.025513829428170466 1.0362448853913156E-4 0.0 1.1842798690186464E-4 0.001657991816626105 64-65 0.0273198562284239 1.0362448853913156E-4 0.0 1.1842798690186464E-4 0.0017394110576211372 66-67 0.029473765240201566 1.258297360832312E-4 0.0 1.1842798690186464E-4 0.0017764198035279699 68-69 0.03206437745367985 1.3323148526459772E-4 0.0 1.258297360832312E-4 0.001835633796978902 70-71 0.03500287187868237 1.3323148526459772E-4 0.0 1.480349836273308E-4 0.0018430355461602687 72-73 0.03842248000047371 1.3323148526459772E-4 0.0 1.5543673280869736E-4 0.0019022495396112007 74-75 0.04288573475683774 1.3323148526459772E-4 0.0 1.6283848199006388E-4 0.001917053037973934 76-77 0.04814097667560798 1.3323148526459772E-4 0.0 1.702402311714304E-4 0.0019392582855180336 78-79 0.0549949964175534 1.3323148526459772E-4 0.0 1.7764198035279696E-4 0.0019540617838807667 80-81 0.06337377649086032 1.3323148526459772E-4 0.0 1.7764198035279696E-4 0.002035481024875799 82-83 0.07357338686278342 1.3323148526459772E-4 0.0 1.7764198035279696E-4 0.0020576862724198982 84-85 0.08657085842526306 1.3323148526459772E-4 0.0 1.7764198035279696E-4 0.0020872932691453645 86-87 0.10281029612918124 1.480349836273308E-4 0.0 1.7764198035279696E-4 0.00213910551341493 88-89 0.12318731162548333 1.5543673280869736E-4 0.0 1.9244547871553004E-4 0.00216131076095903 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 2110 0.0 83.107635 1 TCGACGG 2630 0.0 66.64609 2 ATCTCGA 3975 0.0 56.907143 1 TCTCGAG 4995 0.0 45.171257 2 ACGGCGC 4130 0.0 43.1293 5 GACGGCG 4620 0.0 38.555267 4 CGGCGCG 4735 0.0 37.718903 6 CTCGAGG 6365 0.0 35.22421 3 CGACGGC 5115 0.0 34.917236 3 TCGAGGG 6670 0.0 33.613255 4 CGAGGGC 6965 0.0 32.393932 5 GGGCGCG 7015 0.0 32.02762 8 AGGGCGC 7345 0.0 30.782675 7 CGCGCCG 9980 0.0 26.2724 9 GAGGGCG 8730 0.0 25.953465 6 GCGCGCC 12310 0.0 21.569742 8 GGCGCGC 12445 0.0 21.412094 7 TATCTCG 1440 0.0 17.160812 1 CCATATT 6980 0.0 15.992031 18-19 TCCATAT 6605 0.0 15.497643 18-19 >>END_MODULE