FastQCFastQC Report
Tue 31 May 2016
SRR1051271_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051271_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5487538
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT1000271.8228028671509882No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT114570.20878215330809555No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT59330.10811770232843947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG195400.093.893371
TATCTCG166050.090.696842
TCGACGG203000.090.448342
ACGGCGC210200.087.169455
GACGGCG211350.086.785034
CGGCGCG213150.086.163566
ATATCTC176300.085.720091
ATCTCGA177700.084.643863
CGACGGC222600.082.4633
TCTCGAG182900.081.458354
CTCGAGG192700.077.3403245
CGAGGGC198800.074.871657
AGGGCGC200050.074.522539
TCGAGGG204250.073.013376
GAGGGCG208100.071.731038
CGCGCCG397950.047.499539
GGCGCGC422300.044.8619087
GCGCGCC423450.044.7849358
TCCATAT238350.038.69624718-19
CCATATT242850.038.42901618-19
Produced by FastQC (version 0.11.5)