FastQCFastQC Report
Tue 31 May 2016
SRR1051271_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051271_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5487538
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT66020.12030896186960346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG20300.083.333521
TCGACGG25750.065.8546752
ACGGCGC30850.054.9673355
ATCTCGA21350.050.746111
GACGGCG34150.049.5170634
CGGCGCG37550.045.159586
TCTCGAG28600.038.365562
CGACGGC46750.036.577713
CGCGCCG66900.032.234539
CTCGAGG36950.029.6956713
GGGCGCG41500.026.5540988
AGGGCGC43200.025.6190977
CGAGGGC44400.024.7127275
GGCGCGC91600.023.801757
GCGCGCC92550.023.6087538
TCGAGGG48300.022.5208074
GAGGGCG52050.021.1718566
TCCATAT51100.017.61489118-19
CGGATCC70050.017.15552514-15
CCATATT56600.017.07810418-19
Produced by FastQC (version 0.11.5)