##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051271_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5487538 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26315608201711 33.0 31.0 34.0 31.0 34.0 2 32.53368213577747 34.0 31.0 34.0 31.0 34.0 3 32.661576102069816 34.0 31.0 34.0 31.0 34.0 4 36.18705073204049 37.0 35.0 37.0 35.0 37.0 5 36.098541641078384 37.0 35.0 37.0 35.0 37.0 6 36.10102690860637 37.0 35.0 37.0 35.0 37.0 7 36.06862913751121 37.0 35.0 37.0 35.0 37.0 8 36.076954182367395 37.0 35.0 37.0 35.0 37.0 9 37.85842831521167 39.0 38.0 39.0 35.0 39.0 10-11 37.825591640549916 39.0 38.0 39.0 35.0 39.0 12-13 37.80018762512442 39.0 38.0 39.0 35.0 39.0 14-15 39.17114177614806 41.0 39.0 41.0 36.0 41.0 16-17 39.13607987771566 40.0 38.5 41.0 36.0 41.0 18-19 39.098188750583596 40.0 38.5 41.0 36.0 41.0 20-21 39.05761727754778 40.0 39.0 41.0 35.5 41.0 22-23 38.955525410484626 40.0 38.0 41.0 35.0 41.0 24-25 38.85570806070045 40.0 38.0 41.0 35.0 41.0 26-27 38.67796778810461 40.0 38.0 41.0 34.5 41.0 28-29 38.478491265117434 40.0 38.0 41.0 34.0 41.0 30-31 38.31482861713213 40.0 38.0 41.0 34.0 41.0 32-33 38.48493149386847 40.0 38.0 41.0 34.0 41.0 34-35 38.44381487654391 40.0 38.0 41.0 34.0 41.0 36-37 38.36177025106706 40.0 38.0 41.0 34.0 41.0 38-39 38.24668995458437 40.0 38.0 41.0 33.0 41.0 40-41 38.0817447642276 40.0 37.5 41.0 33.0 41.0 42-43 37.9466459275544 40.0 37.0 41.0 33.0 41.0 44-45 37.838013604643834 40.0 37.0 41.0 33.0 41.0 46-47 37.67589317832514 40.0 37.0 41.0 32.5 41.0 48-49 37.546297082589675 40.0 36.0 41.0 32.0 41.0 50-51 37.548788181512364 39.0 36.0 41.0 32.0 41.0 52-53 37.37352616054777 39.0 36.0 41.0 32.0 41.0 54-55 37.0398020933978 39.0 35.0 41.0 31.0 41.0 56-57 36.79305100028465 39.0 35.0 40.5 31.0 41.0 58-59 36.50885998420421 38.0 35.0 40.0 31.0 41.0 60-61 36.205118032895626 37.5 35.0 40.0 31.0 41.0 62-63 35.82782406244841 37.0 35.0 40.0 30.0 41.0 64-65 35.425334002242906 36.5 34.0 39.0 30.0 41.0 66-67 35.01167800569217 36.0 34.0 39.0 29.0 41.0 68-69 34.619316531384385 35.0 34.0 38.5 29.0 40.0 70-71 34.19588210596446 35.0 34.0 37.0 29.0 39.5 72-73 33.813769672301135 35.0 33.0 37.0 29.0 39.0 74-75 33.47832461479082 35.0 33.0 36.0 28.0 39.0 76-77 32.92258814426433 35.0 32.5 36.0 27.0 37.5 78-79 32.847289531298 35.0 33.0 35.5 27.0 37.0 80-81 32.62822726694557 35.0 33.0 35.0 27.0 37.0 82-83 32.35615598470571 35.0 33.0 35.0 26.5 36.0 84-85 32.14462478437507 35.0 32.0 35.0 26.0 36.0 86-87 31.86438016465672 35.0 32.0 35.0 25.5 36.0 88-89 31.695502427500273 35.0 32.0 35.0 25.0 35.0 90-91 31.459528480713935 34.0 32.0 35.0 25.0 35.0 92-93 31.1899408259223 34.0 31.5 35.0 24.5 35.0 94-95 30.903497980332894 34.0 31.0 35.0 23.5 35.0 96-97 30.646938390221628 34.0 31.0 35.0 21.5 35.0 98-99 30.25049667082032 34.0 31.0 35.0 18.5 35.0 100-101 28.484523934048383 33.0 28.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4016933659504147 1101 2 0.37703505223188927 1101 3 0.32990210994310587 1101 4 0.21448817989982416 1101 5 0.2310108185694233 1101 6 0.21173455653902806 1101 7 0.25647370164845995 1101 8 0.2098420080886072 1101 9 0.28713270113201617 1101 10-11 0.29293509076816093 1101 12-13 0.3344864446267479 1101 14-15 0.4046615781126519 1101 16-17 0.3454716261564883 1101 18-19 0.30800505989732585 1101 20-21 0.361069895833225 1101 22-23 0.3246968933161938 1101 24-25 0.3213503101469186 1101 26-27 0.3387381359744026 1101 28-29 0.3606347284099485 1101 30-31 0.34650757411643696 1101 32-33 0.3105204599216975 1101 34-35 0.30978742096942113 1101 36-37 0.3541572310873633 1101 38-39 0.3603161449631642 1101 40-41 0.30873201750004853 1101 42-43 0.32125472882310646 1101 44-45 0.28115241218227993 1101 46-47 0.24694654740037691 1101 48-49 0.32442242416438205 1101 50-51 0.34088461252805047 1101 52-53 0.3829135103423553 1101 54-55 0.5085009701675176 1101 56-57 0.33938685551671455 1101 58-59 0.3552060391982792 1101 60-61 0.2865659265307343 1101 62-63 0.3742841647683761 1101 64-65 0.3660265250560215 1101 66-67 0.2623520680487488 1101 68-69 0.16572228208504924 1101 70-71 0.15488990680065484 1101 72-73 0.13724697696633115 1101 74-75 0.16185880625688753 1101 76-77 0.11882896942807264 1101 78-79 0.11841709766419939 1101 80-81 0.1131279078455023 1101 82-83 -0.09885280804228103 1101 84-85 -0.05644529257789799 1101 86-87 0.17424228784059537 1101 88-89 0.15845691473287005 1101 90-91 0.015717529015947918 1101 92-93 -0.12507544488753553 1101 94-95 -0.09859166302180711 1101 96-97 -0.02660550371136594 1101 98-99 0.01085818995725063 1101 100-101 -0.03229152196974283 1102 1 0.3625009511293342 1102 2 0.35022755779208126 1102 3 0.2753363159661859 1102 4 0.18658771869481683 1102 5 0.23602516957841146 1102 6 0.22190273828068285 1102 7 0.22177056344593638 1102 8 0.19195221096427417 1102 9 0.24129508972517044 1102 10-11 0.2622068732583145 1102 12-13 0.23055158920067953 1102 14-15 0.32174606468199585 1102 16-17 0.3184223795891157 1102 18-19 0.3341022162691303 1102 20-21 0.32286432007866495 1102 22-23 0.3367152813217089 1102 24-25 0.33671017249017154 1102 26-27 0.30276022685088577 1102 28-29 0.4043534362671082 1102 30-31 0.31738636733540204 1102 32-33 0.32670207742162205 1102 34-35 0.40073039385839593 1102 36-37 0.3917066896773207 1102 38-39 0.38709482247114835 1102 40-41 0.4018011202883969 1102 42-43 0.3807130315934515 1102 44-45 0.4067017684113665 1102 46-47 0.34519910802385567 1102 48-49 0.34562436186162415 1102 50-51 0.43461075905547375 1102 52-53 0.45623442422527916 1102 54-55 0.569096054104179 1102 56-57 0.4645858455337688 1102 58-59 0.4100167095815266 1102 60-61 0.36275137567221094 1102 62-63 0.4332227710051697 1102 64-65 0.4365999500582092 1102 66-67 0.4167511726742319 1102 68-69 0.39330951641799317 1102 70-71 0.446866040030109 1102 72-73 0.3981455135783776 1102 74-75 0.36543699197079604 1102 76-77 0.3343450755088 1102 78-79 0.32340755050153547 1102 80-81 0.3916477631352393 1102 82-83 0.24463493878783993 1102 84-85 0.20780549271026416 1102 86-87 0.4681218844136019 1102 88-89 0.46945159438879003 1102 90-91 0.37243246639478755 1102 92-93 0.33559250691464726 1102 94-95 0.3754064408436335 1102 96-97 0.44823513106608814 1102 98-99 0.5011982925177776 1102 100-101 0.6836894265042979 1103 1 0.2911205202010265 1103 2 0.28462931452333606 1103 3 0.24071681881259366 1103 4 0.1340635833645294 1103 5 0.1506995903297934 1103 6 0.13551155145690075 1103 7 0.17680713458538833 1103 8 0.14241011916995916 1103 9 0.1351012274113046 1103 10-11 0.09892618212470694 1103 12-13 0.1809567221136703 1103 14-15 0.24605390517107395 1103 16-17 0.2267459524870432 1103 18-19 0.18932836190525393 1103 20-21 0.19760956595712287 1103 22-23 0.1722465986764874 1103 24-25 0.14105062041195282 1103 26-27 0.21393282303125005 1103 28-29 0.21575287896001782 1103 30-31 0.2119006989836194 1103 32-33 0.12609163835581683 1103 34-35 0.149522117645553 1103 36-37 0.22915595269131472 1103 38-39 0.1575819558718976 1103 40-41 0.10598632844646261 1103 42-43 0.21665626139073169 1103 44-45 0.18142539780366462 1103 46-47 0.1876136750036963 1103 48-49 0.2763144202829366 1103 50-51 0.30591795977457537 1103 52-53 0.30692939613752657 1103 54-55 0.38042407480454443 1103 56-57 0.3038814568670247 1103 58-59 0.267765860626362 1103 60-61 0.20874932515108213 1103 62-63 0.2672796775511941 1103 64-65 0.22716357033996104 1103 66-67 0.23541874399665375 1103 68-69 0.22642810477590558 1103 70-71 0.252703822226529 1103 72-73 0.33820349865636246 1103 74-75 0.3594951911265838 1103 76-77 0.2822143299473652 1103 78-79 0.2859875884669094 1103 80-81 0.3269504762266493 1103 82-83 0.2576636231944036 1103 84-85 0.3260368390307278 1103 86-87 0.47050665191423136 1103 88-89 0.45014959561995127 1103 90-91 0.318006277867827 1103 92-93 0.25619565015976065 1103 94-95 0.3491611936992456 1103 96-97 0.45520060579464783 1103 98-99 0.4829135952519117 1103 100-101 0.6279557023019215 1104 1 0.39185462233697166 1104 2 0.38460122500610794 1104 3 0.27333319831467406 1104 4 0.1803132709769244 1104 5 0.19674516325927272 1104 6 0.18592668640346943 1104 7 0.25041092724276126 1104 8 0.23007837723050528 1104 9 0.2392525492425719 1104 10-11 0.2508798612871246 1104 12-13 0.26841959603545007 1104 14-15 0.34541771176034075 1104 16-17 0.2932233265911961 1104 18-19 0.35495187409976836 1104 20-21 0.33671561791342697 1104 22-23 0.343357122191712 1104 24-25 0.3459949445397186 1104 26-27 0.37227934798266205 1104 28-29 0.3762580747175832 1104 30-31 0.37424050024055333 1104 32-33 0.3503474904630721 1104 34-35 0.39849591156038144 1104 36-37 0.3914058730200054 1104 38-39 0.42773211272266565 1104 40-41 0.396553761133589 1104 42-43 0.4300537192855316 1104 44-45 0.3586545560092702 1104 46-47 0.3555548595080751 1104 48-49 0.3864064327743435 1104 50-51 0.4163036181290991 1104 52-53 0.43950939579848836 1104 54-55 0.35894772736200764 1104 56-57 0.25704265106642055 1104 58-59 0.33522451114626506 1104 60-61 0.23111007312034104 1104 62-63 0.2106728192097691 1104 64-65 0.28513158328239996 1104 66-67 0.15317142942039652 1104 68-69 0.15326170874369183 1104 70-71 0.318350320207621 1104 72-73 0.2743684019034873 1104 74-75 0.31838705876531037 1104 76-77 0.2573085345817603 1104 78-79 0.2503142167964114 1104 80-81 0.3448343758882757 1104 82-83 0.19091576431951296 1104 84-85 0.24581813216875048 1104 86-87 0.44915804022785366 1104 88-89 0.4540563373519326 1104 90-91 0.39787980068647144 1104 92-93 0.2970456943752495 1104 94-95 0.4823560417776349 1104 96-97 0.5076015453405098 1104 98-99 0.6089682723387426 1104 100-101 0.7271442289910688 1105 1 -0.10595263516108133 1105 2 0.07694198684314557 1105 3 0.053752825340232846 1105 4 0.0346458160147165 1105 5 0.024129493607546237 1105 6 0.045866590592133605 1105 7 0.0921541553575338 1105 8 -0.01344056985380604 1105 9 -0.018711710456763342 1105 10-11 -0.012647386605863176 1105 12-13 0.021882530643118514 1105 14-15 0.0384124496374838 1105 16-17 0.03853260223127819 1105 18-19 3.2167225829482504E-4 1105 20-21 0.01948560423835488 1105 22-23 0.08392061329016087 1105 24-25 -0.004653338024127152 1105 26-27 0.04527769853169872 1105 28-29 0.05675154808512417 1105 30-31 0.10455677223958304 1105 32-33 0.1157399247831492 1105 34-35 0.11233761853484481 1105 36-37 0.14061082154684357 1105 38-39 0.1378322189457819 1105 40-41 0.13255339018203927 1105 42-43 0.17927643029867824 1105 44-45 0.16734636503262834 1105 46-47 -0.07198194928802337 1105 48-49 0.14979047209050833 1105 50-51 0.1237659402874911 1105 52-53 0.10461297150451543 1105 54-55 0.17064483232511662 1105 56-57 0.0032386798555634755 1105 58-59 0.13210085452243447 1105 60-61 0.10626731558922131 1105 62-63 0.16445818721319228 1105 64-65 0.17427534204185946 1105 66-67 0.19009447113311495 1105 68-69 0.15745621371993224 1105 70-71 0.10467444997957642 1105 72-73 0.14795676708788363 1105 74-75 0.198543128729888 1105 76-77 0.11462647797042536 1105 78-79 0.22707782111563546 1105 80-81 0.3033696419336849 1105 82-83 0.26467469638399166 1105 84-85 0.20146749172534584 1105 86-87 0.40795752619544245 1105 88-89 0.38063775630724805 1105 90-91 0.36004188907033097 1105 92-93 0.22343547514284268 1105 94-95 0.3340013232178052 1105 96-97 0.4762588978043887 1105 98-99 0.6377193950599 1105 100-101 0.8029104708788815 1106 1 -0.16720354200394638 1106 2 -0.03372794748695185 1106 3 -0.042914061107723 1106 4 -0.02482670537440157 1106 5 -0.3558270571422426 1106 6 -0.2418783207016162 1106 7 -0.16012095834496876 1106 8 -0.35220512808470517 1106 9 -0.486864392691281 1106 10-11 -0.09292232416964197 1106 12-13 -0.0684977876350743 1106 14-15 -0.26912626603166956 1106 16-17 -0.11857898978454529 1106 18-19 -0.20432707245041115 1106 20-21 -0.19036551639034371 1106 22-23 -0.1552272446765457 1106 24-25 -0.11269990530612972 1106 26-27 -0.09487413053579274 1106 28-29 -0.13492178799263854 1106 30-31 -0.18518120827783946 1106 32-33 -0.19496726845438417 1106 34-35 -0.09807636568752542 1106 36-37 -0.11343590379730983 1106 38-39 -0.13931275472977234 1106 40-41 -0.0834012096314325 1106 42-43 -0.16821230777306795 1106 44-45 -0.11849520308977901 1106 46-47 -0.21988583283507523 1106 48-49 -0.07781723137485841 1106 50-51 -0.09171495341574598 1106 52-53 -0.1468183859676273 1106 54-55 -0.06608494137093146 1106 56-57 -0.17282038213948425 1106 58-59 -0.12304671821874535 1106 60-61 -0.22980042237494303 1106 62-63 -0.19296550795013445 1106 64-65 -0.1377034555145471 1106 66-67 -0.1674280961993091 1106 68-69 -0.14946852597305593 1106 70-71 -0.10672806967854598 1106 72-73 -0.05345506592468752 1106 74-75 -0.09163363992568208 1106 76-77 -0.19658738856994518 1106 78-79 -0.14084733161325147 1106 80-81 -0.10723341889222837 1106 82-83 -0.22691334651817385 1106 84-85 -0.21017845187971673 1106 86-87 0.008741674839896518 1106 88-89 0.03571489120549387 1106 90-91 -0.14658247763950882 1106 92-93 -0.26810529811829653 1106 94-95 -0.10042113715113032 1106 96-97 -0.05491542065257349 1106 98-99 0.029885678403307026 1106 100-101 0.07604722994956248 1107 1 -0.18144831057088595 1107 2 -0.1441248724219193 1107 3 -0.20121576668404373 1107 4 -0.12157114964627169 1107 5 -0.1470846518306459 1107 6 -0.17339025907433125 1107 7 -0.14571562417292938 1107 8 -0.13281259601760098 1107 9 -0.2466949014217903 1107 10-11 -0.27042641417036606 1107 12-13 -0.3071704882033046 1107 14-15 -0.26280883099422425 1107 16-17 -0.27238121818778893 1107 18-19 -0.2887777676642429 1107 20-21 -0.2383389760391168 1107 22-23 -0.29583644338155324 1107 24-25 -0.3718697617131639 1107 26-27 -0.4021922495410095 1107 28-29 -0.36544994564864197 1107 30-31 -0.4634486464359924 1107 32-33 -0.4930378098934014 1107 34-35 -0.39436989960346835 1107 36-37 -0.342848650017082 1107 38-39 -0.25649656057036196 1107 40-41 -0.25749416136942216 1107 42-43 -0.3160143494370047 1107 44-45 -0.4153777531966085 1107 46-47 -0.38927477141085376 1107 48-49 -0.29126098034660686 1107 50-51 -0.19636640884624512 1107 52-53 -0.2578226450907479 1107 54-55 -0.36640688871175087 1107 56-57 -0.452134444867788 1107 58-59 -0.3264730124220989 1107 60-61 -0.3553819513765575 1107 62-63 -0.29262571359490863 1107 64-65 -0.2805034255729808 1107 66-67 -0.3090778434361212 1107 68-69 -0.29641176995051666 1107 70-71 -0.21174955224916658 1107 72-73 -0.19841870784598115 1107 74-75 -0.20250479246315223 1107 76-77 -0.3175451782236891 1107 78-79 -0.2376680049993638 1107 80-81 -0.18328474610034107 1107 82-83 -0.17260775608832546 1107 84-85 -0.34523060118660354 1107 86-87 -0.0916941964222957 1107 88-89 -0.14177496145093826 1107 90-91 -0.3019662646695451 1107 92-93 -0.3601807473424614 1107 94-95 -0.30704917099976825 1107 96-97 -0.3800162433409717 1107 98-99 -0.38285626297220077 1107 100-101 -0.1970896409755376 1108 1 -0.35300851297254354 1108 2 -0.28164789081816366 1108 3 -0.302660718291925 1108 4 -0.17444614238896605 1108 5 -0.36172114731527927 1108 6 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10-11 0.02517810631582762 2305 12-13 0.03613182583691099 2305 14-15 0.13720096151441652 2305 16-17 0.04029670624285586 2305 18-19 0.11807183945759903 2305 20-21 0.12501106225552405 2305 22-23 0.169514310669463 2305 24-25 0.10930347393223627 2305 26-27 0.19762240107056073 2305 28-29 0.12805407178672112 2305 30-31 0.17027544022257501 2305 32-33 0.17693861327921923 2305 34-35 0.15191277498569633 2305 36-37 0.19834542166742608 2305 38-39 0.1387267802500034 2305 40-41 0.22414064721468918 2305 42-43 0.28375161716676445 2305 44-45 0.2992931755390771 2305 46-47 0.3238049840955526 2305 48-49 0.30522415764141897 2305 50-51 0.2609783625828399 2305 52-53 0.25835373561715613 2305 54-55 0.2383345556359302 2305 56-57 0.3116367827125046 2305 58-59 0.3680004991802477 2305 60-61 0.470206605848297 2305 62-63 0.37816339627600115 2305 64-65 0.3638505267010217 2305 66-67 0.5117523543844911 2305 68-69 0.5487374128107163 2305 70-71 0.557799016683731 2305 72-73 0.5130739927758938 2305 74-75 0.46613726711349557 2305 76-77 0.43605626452599466 2305 78-79 0.47474346451517846 2305 80-81 0.531240269879639 2305 82-83 0.560981037971203 2305 84-85 0.5626699796631485 2305 86-87 0.7263565364918598 2305 88-89 0.6465039190086763 2305 90-91 0.681743712181774 2305 92-93 0.8563951048485094 2305 94-95 0.9908159029537664 2305 96-97 1.0276466075499577 2305 98-99 1.0282437544720757 2305 100-101 1.1724987346442433 2306 1 -0.5605520403782904 2306 2 -0.9715040391542082 2306 3 -0.7866987244531884 2306 4 -0.3934199302665533 2306 5 -0.24343595704264942 2306 6 -0.2391479510682899 2306 7 -0.11487504249414826 2306 8 -0.09284228927268856 2306 9 -0.13491802098609895 2306 10-11 -0.40533612066879243 2306 12-13 -0.2810325984195501 2306 14-15 -0.17372999010054002 2306 16-17 -0.2588339466034242 2306 18-19 -0.2046726744541445 2306 20-21 -0.17514209036686879 2306 22-23 -0.09482842136439018 2306 24-25 -0.17066418259461358 2306 26-27 -0.2034422181186386 2306 28-29 -0.16368411768411306 2306 30-31 -0.06046136727088225 2306 32-33 -0.11649687081230553 2306 34-35 -0.2582194449104378 2306 36-37 -0.27878663649592283 2306 38-39 -0.1350530805528649 2306 40-41 -0.23756541367272632 2306 42-43 -0.12110621683329015 2306 44-45 -0.196642915435973 2306 46-47 -0.14342561899707107 2306 48-49 -0.05210629102877817 2306 50-51 -0.0046611620728782555 2306 52-53 -0.05082283165632617 2306 54-55 -0.16261668095158655 2306 56-57 -0.09967843593161518 2306 58-59 -0.138779862654971 2306 60-61 -0.20621767344088227 2306 62-63 -0.03540754750833486 2306 64-65 -0.04472872940835515 2306 66-67 -0.017667616108781203 2306 68-69 0.05726226275034918 2306 70-71 0.05582938276018723 2306 72-73 0.011115026224771896 2306 74-75 -0.011246566743231767 2306 76-77 -0.018887144934858213 2306 78-79 0.09245840744241463 2306 80-81 0.1947444333422368 2306 82-83 0.17816561970584388 2306 84-85 0.036824607107611484 2306 86-87 0.24148287056888407 2306 88-89 0.13990582786685124 2306 90-91 0.24370898764829008 2306 92-93 0.2567618979505326 2306 94-95 0.38667024329939537 2306 96-97 0.23479091442728262 2306 98-99 0.3066553112617214 2306 100-101 0.7068852280954019 2307 1 -1.6195537117946124 2307 2 -2.203934235544615 2307 3 -1.7104913094794192 2307 4 -0.8998490425388113 2307 5 -0.5084500885253291 2307 6 -0.427536097773185 2307 7 -0.3566702816382161 2307 8 -0.4886755714025526 2307 9 -0.4147256048705472 2307 10-11 -0.3301443439776861 2307 12-13 -0.3514693563715383 2307 14-15 -0.6708816074257484 2307 16-17 -0.5164768917262208 2307 18-19 -0.4269091184872167 2307 20-21 -0.43656765980910706 2307 22-23 -0.5045597523651892 2307 24-25 -0.4348597798973941 2307 26-27 -0.4362755726310823 2307 28-29 -0.39631012852503034 2307 30-31 -0.7616893660398389 2307 32-33 -0.4566032799518567 2307 34-35 -0.4668486667828873 2307 36-37 -0.39906433508649997 2307 38-39 -0.3951837547357968 2307 40-41 -0.3140317043911267 2307 42-43 -0.3269877130513734 2307 44-45 -0.35559536399944136 2307 46-47 -0.3982497354131027 2307 48-49 -0.3587083787529508 2307 50-51 -0.32790443964237426 2307 52-53 -0.3997778791054003 2307 54-55 -0.3474617545542671 2307 56-57 -0.2067500347236404 2307 58-59 -0.4188466227527812 2307 60-61 -0.4108126976519131 2307 62-63 -0.2931324868305367 2307 64-65 -0.25616565251685586 2307 66-67 -0.3099677878688567 2307 68-69 -0.22498807104000917 2307 70-71 -0.27510920589030263 2307 72-73 -0.3081675503572754 2307 74-75 -0.33846129127688585 2307 76-77 -0.24141913877639354 2307 78-79 -0.25571862183890204 2307 80-81 -0.2867695529026477 2307 82-83 -0.21235870090737308 2307 84-85 -0.18665473421634715 2307 86-87 -0.20465158249770354 2307 88-89 -0.19592523889702917 2307 90-91 -0.2073518796165139 2307 92-93 -0.15272254229380167 2307 94-95 -0.037863027500435464 2307 96-97 -0.11648150307109972 2307 98-99 -0.0228336204835351 2307 100-101 0.15326901955687688 2308 1 -1.2570672621419803 2308 2 -1.2357514848146138 2308 3 -0.7943600862472842 2308 4 -0.5315619124281312 2308 5 -0.6574508334100884 2308 6 -0.5834440884776342 2308 7 -1.5223508384712972 2308 8 -0.5911937673029257 2308 9 -0.6151973826617336 2308 10-11 -0.7931014200099682 2308 12-13 -0.8799882204831206 2308 14-15 -0.6864353388953717 2308 16-17 -0.8734301678387624 2308 18-19 -0.6118453944010156 2308 20-21 -0.7064440256873894 2308 22-23 -0.5850559466229655 2308 24-25 -0.5456237080843849 2308 26-27 -0.5092966952756441 2308 28-29 -1.357577943183884 2308 30-31 -0.6136997305754264 2308 32-33 -0.49404305491370337 2308 34-35 -1.203738619840621 2308 36-37 -0.6889330748260178 2308 38-39 -0.5075272559924713 2308 40-41 -0.9941442365505111 2308 42-43 -0.4679501792597307 2308 44-45 -0.5595417223529893 2308 46-47 -0.5239593156673408 2308 48-49 -0.41119327530843464 2308 50-51 -0.509308682175174 2308 52-53 -0.5977404858383082 2308 54-55 -1.070379436611077 2308 56-57 -1.7534477308407546 2308 58-59 -1.1392054987553024 2308 60-61 -0.7810087764937705 2308 62-63 -0.44824004044345855 2308 64-65 -0.3136793356690717 2308 66-67 -0.3280777085483777 2308 68-69 -0.27587733364335065 2308 70-71 -0.16402015114291402 2308 72-73 -0.2661450789911157 2308 74-75 -0.0784195794314897 2308 76-77 -1.0726339839252788 2308 78-79 -0.6097922471483912 2308 80-81 -0.10559580202387764 2308 82-83 7.316935335026642E-4 2308 84-85 0.0019227007606303914 2308 86-87 -0.3306626363100982 2308 88-89 -0.2757022752501008 2308 90-91 -0.8980395935190089 2308 92-93 -0.049822594591439895 2308 94-95 0.1599088407158078 2308 96-97 0.05758489189733851 2308 98-99 -0.45480443272455773 2308 100-101 0.36972215717292656 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 7.0 12 74.0 13 403.0 14 1558.0 15 3731.0 16 6249.0 17 9493.0 18 14033.0 19 19821.0 20 20095.0 21 20267.0 22 21995.0 23 24458.0 24 28782.0 25 35468.0 26 44240.0 27 55704.0 28 69985.0 29 87896.0 30 108334.0 31 135655.0 32 171832.0 33 225191.0 34 315501.0 35 479788.0 36 846337.0 37 1458636.0 38 1142205.0 39 139741.0 40 57.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 25.269972337782782 28.479887053998475 21.70956258036546 24.54057802785328 2 26.426045817958236 27.035831806074135 24.914555793085338 21.62356658288229 3 24.60870373373813 25.207388387853918 25.55497646482115 24.628931413586805 4 25.473687701695972 25.53036166187003 25.115220347810464 23.880730288623532 5 25.503135559723788 26.0829403063706 24.670029559709576 23.743894574196037 6 25.253146310786367 25.0681817601992 24.073837848594398 25.604834080420037 7 25.68352146263042 25.204909742766247 24.678899717869836 24.4326690767335 8 24.739382214756418 25.015753877239668 25.02608637972074 25.218777528283177 9 24.54315578315813 24.54845870771191 25.32425287988894 25.58413262924102 10-11 24.915116760922658 25.098022100256983 24.95675656369031 25.030104575130046 12-13 24.89973645740585 25.078824055523626 25.058122604344607 24.963316882725913 14-15 24.824894151074673 25.069548493331617 25.123798687134375 24.981758668459335 16-17 24.971544616183067 25.208955272838203 24.99873349396396 24.82076661701477 18-19 24.708093137578274 24.999371302759087 25.167379250950063 25.125156308712576 20-21 24.614471918007673 25.06573986366928 25.179944084214085 25.13984413410896 22-23 24.73893574859983 25.04154868722549 25.419550625435306 24.79996493873938 24-25 24.74323630860918 24.80917665050398 25.661881762598004 24.785705278288837 26-27 24.650480780269767 24.819372549219704 25.72431389085597 24.805832779654555 28-29 24.71656688299926 24.941850060992742 25.656478369716986 24.68510468629101 30-31 24.652549103076826 24.79861642871539 25.732395839445665 24.816438628762118 32-33 24.676339371135107 24.830351972050124 25.755812533781086 24.73749612303368 34-35 24.724302592528744 24.93488883357163 25.64319372366989 24.697614850229737 36-37 24.740812728768347 24.856821037047943 25.547112384460934 24.85525384972277 38-39 24.778735017415826 24.865085216721962 25.58003242984377 24.776147336018447 40-41 24.90166810689967 24.95260169496776 25.338840478188946 24.806889719943626 42-43 24.905740971634273 24.92137639866913 25.29738290650561 24.875499723190984 44-45 24.813276919449123 25.027152431564026 25.372134097294634 24.787436551692217 46-47 24.85542696925288 25.12934762365199 25.213037249127023 24.802188157968107 48-49 24.846825662072863 25.068099756211254 25.220854962644452 24.86421961907143 50-51 24.79569161981202 25.10555735559371 25.317428325781073 24.781322698813202 52-53 24.884829954708287 25.220280934728834 25.176099008334884 24.718790102227995 54-55 24.87430610958867 25.125775894399272 25.15811280031227 24.841805195699784 56-57 24.82874833850809 25.17325620341946 25.27688190951935 24.721113548553102 58-59 24.841586518398596 25.223597540463498 25.211907416404223 24.72290852473368 60-61 24.8556092003372 25.140181261614952 25.20036307721241 24.80384646083544 62-63 24.85049761842196 25.1352336876756 25.268809072483872 24.74545962141857 64-65 24.860119419674177 25.26001642266532 25.237246648679246 24.64261750898126 66-67 24.89742212263496 25.174285809045877 25.13275534492882 24.79553672339034 68-69 24.849513570566618 25.225866317463314 25.203907471802474 24.720712640167594 70-71 24.92851074562035 25.3241162065757 25.08519303192069 24.662180015883262 72-73 24.924720339066443 25.261337598026657 25.058541735838546 24.75540032706835 74-75 24.858488451469494 25.28350600943447 25.145119724000086 24.71288581509595 76-77 24.89908953705651 25.417700979929432 24.988236983506994 24.694972499507063 78-79 24.827800736869612 25.287396643084747 25.090432175594955 24.79437044445068 80-81 24.848110391217336 25.361081782030485 25.14411745303632 24.646690373715863 82-83 24.86290755526431 25.45830206551645 25.05182652038127 24.62696385883797 84-85 24.854114905445755 25.39874894715991 24.99988154979519 24.74725459759914 86-87 24.853067076710904 25.412243158953977 25.054350420899134 24.680339343435982 88-89 24.935435526824598 25.494101362031568 24.988710784326233 24.5817523268176 90-91 24.95227367901598 25.380015591691574 24.97679287141155 24.690917857880894 92-93 24.835982912555686 25.511057964427764 24.941813614775878 24.711145508240673 94-95 24.97693865627901 25.582082529542394 24.87026969107093 24.57070912310767 96-97 24.885495098166064 25.53281635589585 24.885932452768436 24.695756093169653 98-99 24.792484352727946 25.58778636248168 24.940064196366386 24.679665088423988 100-101 24.98342380046128 25.689733453253567 24.588574490575144 24.738268255710008 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1356.0 1 818.5 2 227.5 3 174.5 4 153.0 5 138.0 6 145.5 7 132.0 8 122.5 9 136.5 10 142.5 11 147.5 12 187.0 13 260.5 14 366.0 15 475.5 16 1258.5 17 1523.5 18 1079.0 19 1163.0 20 1411.0 21 1848.5 22 2405.0 23 3219.5 24 4342.0 25 5619.0 26 7406.0 27 9905.0 28 12339.0 29 16000.5 30 20879.5 31 24222.5 32 28213.5 33 36429.5 34 44676.0 35 52908.5 36 66740.0 37 78709.0 38 90780.0 39 108298.0 40 125322.0 41 136047.0 42 148156.0 43 164153.0 44 181939.0 45 200870.0 46 213302.5 47 229881.0 48 241689.0 49 242109.0 50 239704.0 51 238129.5 52 242206.0 53 246640.5 54 246414.5 55 240869.5 56 226443.0 57 205620.0 58 190037.5 59 175336.0 60 153302.0 61 129418.0 62 106182.5 63 80596.5 64 58798.5 65 44270.0 66 32038.0 67 22617.0 68 17012.5 69 13681.0 70 11797.0 71 10540.5 72 9932.5 73 8541.0 74 6514.0 75 5654.5 76 5024.5 77 3565.5 78 2297.5 79 1565.5 80 1002.0 81 795.5 82 659.0 83 542.5 84 368.0 85 193.0 86 74.0 87 3.0 88 2.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04444616146621672 2 7.107012288570941E-4 3 1.4578486745786543E-4 4 1.6400797589009863E-4 5 1.8223108432233178E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.6446216864466356E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 2.7334662648349772E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5487538.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.21358497136368 #Duplication Level Percentage of deduplicated Percentage of total 1 83.9649601931989 43.8411158366476 2 8.079064676535507 8.436738599548594 3 2.3496515584201756 3.680511938960069 4 1.1241671065820624 2.347871789661383 5 0.7196901968653668 1.8788802623543648 6 0.5310308622596225 1.6636215029405594 7 0.4228580245712253 1.5455253377740863 8 0.34371623667457685 1.4357325543716295 9 0.28201724369602815 1.325261818540111 >10 1.9393264312917675 18.32170658790357 >50 0.11312702073056581 4.18887542391577 >100 0.12993675340452676 11.001691874941177 >500 2.7919798836001895E-4 0.09133065121620472 >1k 1.3959822510281565E-4 0.12083213409978821 >5k 3.489955627565159E-5 0.12030368712500836 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6602 0.12030896186960346 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.644621686446636E-5 0.0 0.0 0.0 0.0 2 3.644621686446636E-5 0.0 0.0 0.0 1.822310843223318E-5 3 3.644621686446636E-5 0.0 0.0 0.0 3.644621686446636E-5 4 3.644621686446636E-5 0.0 0.0 0.0 3.644621686446636E-5 5 3.644621686446636E-5 0.0 0.0 0.0 3.644621686446636E-5 6 3.644621686446636E-5 0.0 0.0 0.0 3.644621686446636E-5 7 5.466932529669954E-5 0.0 0.0 0.0 5.466932529669954E-5 8 5.466932529669954E-5 0.0 0.0 1.822310843223318E-5 9.11155421611659E-5 9 5.466932529669954E-5 0.0 0.0 1.822310843223318E-5 1.0933865059339908E-4 10-11 6.378087951281614E-5 1.822310843223318E-5 0.0 1.822310843223318E-5 1.3667331324174886E-4 12-13 9.11155421611659E-5 3.644621686446636E-5 0.0 1.822310843223318E-5 2.3690040961903136E-4 14-15 1.1845020480951568E-4 3.644621686446636E-5 0.0 1.822310843223318E-5 2.5512351805126454E-4 16-17 1.2756175902563227E-4 3.644621686446636E-5 0.0 1.822310843223318E-5 3.006812891318475E-4 18-19 1.4578486745786545E-4 3.644621686446636E-5 0.0 1.822310843223318E-5 3.462390602124304E-4 20-21 1.5489642167398204E-4 3.644621686446636E-5 0.0 1.822310843223318E-5 4.1001993972524653E-4 22-23 1.731195301062152E-4 3.644621686446636E-5 0.0 3.644621686446636E-5 4.555777108058295E-4 24-25 1.822310843223318E-4 5.466932529669954E-5 0.0 3.644621686446636E-5 4.555777108058295E-4 26-27 2.0045419275456497E-4 5.466932529669954E-5 0.0 3.644621686446636E-5 4.646892650219461E-4 28-29 2.2778885540291474E-4 5.466932529669954E-5 0.0 3.644621686446636E-5 4.829123734541793E-4 30-31 2.5512351805126454E-4 5.466932529669954E-5 0.0 3.644621686446636E-5 5.284701445347623E-4 32-33 2.733466264834977E-4 5.466932529669954E-5 0.0 3.644621686446636E-5 5.649163613992286E-4 34-35 2.733466264834977E-4 5.466932529669954E-5 0.0 4.5557771080582954E-5 6.01362578263695E-4 36-37 2.733466264834977E-4 5.466932529669954E-5 0.0 5.466932529669954E-5 6.924781204248608E-4 38-39 2.824581806996143E-4 5.466932529669954E-5 0.0 5.466932529669954E-5 7.015896746409774E-4 40-41 2.915697349157309E-4 5.466932529669954E-5 0.0 5.466932529669954E-5 7.744821083699101E-4 42-43 2.915697349157309E-4 5.466932529669954E-5 0.0 5.466932529669954E-5 8.564860963149594E-4 44-45 3.4623906021243045E-4 5.466932529669954E-5 0.0 6.378087951281614E-5 9.384900842600087E-4 46-47 3.9179683129301335E-4 5.466932529669954E-5 0.0 7.289243372893273E-5 9.931594095567084E-4 48-49 4.1001993972524653E-4 5.466932529669954E-5 0.0 7.289243372893273E-5 0.0010660518432856411 50-51 4.646892650219461E-4 5.466932529669954E-5 0.0 7.289243372893273E-5 0.0010751633975017577 52-53 7.289243372893272E-4 5.466932529669954E-5 0.0 7.289243372893273E-5 0.001111609614366224 54-55 0.0012300598191757398 5.466932529669954E-5 0.0 7.289243372893273E-5 0.001157167385446807 56-57 0.0017129721926299188 5.466932529669954E-5 0.0 8.200398794504932E-5 0.00120272515652739 58-59 0.00240545031305478 5.466932529669954E-5 0.0 9.11155421611659E-5 0.001248282927607973 60-61 0.0032710479635858557 5.466932529669954E-5 0.0 9.11155421611659E-5 0.001375844686633605 62-63 0.004282430481574798 5.466932529669954E-5 0.0 9.11155421611659E-5 0.0014396255661464211 64-65 0.005667386722424519 5.466932529669954E-5 0.0 9.11155421611659E-5 0.0015034064456592373 66-67 0.007599036216241237 5.466932529669954E-5 0.0 1.0022709637728248E-4 0.0015489642167398202 68-69 0.009922482541350967 5.466932529669954E-5 0.0 1.0933865059339908E-4 0.0015671873251720534 70-71 0.01266506036040206 7.289243372893271E-5 0.0 1.0933865059339908E-4 0.0015854104336042866 72-73 0.01692015617932851 9.11155421611659E-5 0.0 1.2756175902563227E-4 0.0016400797589009861 74-75 0.02212285363673108 9.11155421611659E-5 0.0 1.4578486745786545E-4 0.001667414421549336 76-77 0.028582945575957742 1.0022709637728248E-4 0.0 1.4578486745786545E-4 0.0017494184094943852 78-79 0.036655782611437045 1.0933865059339908E-4 0.0 1.5489642167398204E-4 0.0018587570600877843 80-81 0.04720696239370005 1.0933865059339908E-4 0.0 1.640079758900986E-4 0.0018952032769522507 82-83 0.061038301693765035 1.0933865059339908E-4 0.0 1.640079758900986E-4 0.0019134263853844838 84-85 0.07898806349951472 1.1845020480951568E-4 0.0 1.822310843223318E-4 0.0019134263853844838 86-87 0.10109269402781357 1.2756175902563227E-4 0.0 1.822310843223318E-4 0.0019407610480328338 88-89 0.12991254001339034 1.2756175902563227E-4 0.0 1.822310843223318E-4 0.0020136534817617666 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 2030 0.0 83.33352 1 TCGACGG 2575 0.0 65.854675 2 ACGGCGC 3085 0.0 54.967335 5 ATCTCGA 2135 0.0 50.74611 1 GACGGCG 3415 0.0 49.517063 4 CGGCGCG 3755 0.0 45.15958 6 TCTCGAG 2860 0.0 38.36556 2 CGACGGC 4675 0.0 36.57771 3 CGCGCCG 6690 0.0 32.23453 9 CTCGAGG 3695 0.0 29.695671 3 GGGCGCG 4150 0.0 26.554098 8 AGGGCGC 4320 0.0 25.619097 7 CGAGGGC 4440 0.0 24.712727 5 GGCGCGC 9160 0.0 23.80175 7 GCGCGCC 9255 0.0 23.608753 8 TCGAGGG 4830 0.0 22.520807 4 GAGGGCG 5205 0.0 21.171856 6 TCCATAT 5110 0.0 17.614891 18-19 CGGATCC 7005 0.0 17.155525 14-15 CCATATT 5660 0.0 17.078104 18-19 >>END_MODULE