FastQCFastQC Report
Tue 31 May 2016
SRR1051270_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051270_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8244706
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT3295913.9976076769747766No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT600230.72801868253398No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT158280.19197773698661905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG474750.094.09511
TCGACGG485800.091.925482
TATCTCG445950.091.4712
ATATCTC454350.089.9471361
ACGGCGC500200.089.2411045
CGACGGC502550.088.899413
GACGGCG503000.088.8104254
CGGCGCG505800.088.337576
ATCTCGA472900.086.177833
TCTCGAG476600.084.153584
CTCGAGG492050.081.569135
TCGAGGG492650.081.5083546
CGAGGGC502100.080.012127
AGGGCGC504150.079.875189
GAGGGCG518900.077.64138
CGCGCCG990800.046.0882539
GCGCGCC1009850.045.261168
GGCGCGC1009600.045.164187
CCATATT551350.040.3130318-19
TCCATAT551800.040.26724618-19
Produced by FastQC (version 0.11.5)