##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1051270_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8244706
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07555660565701	33.0	31.0	34.0	30.0	34.0
2	32.2998305821942	34.0	31.0	34.0	30.0	34.0
3	32.39031591908796	34.0	31.0	34.0	30.0	34.0
4	35.81325568188848	37.0	35.0	37.0	35.0	37.0
5	35.80576978730351	37.0	35.0	37.0	35.0	37.0
6	35.81134293933586	37.0	35.0	37.0	35.0	37.0
7	35.756220537154384	37.0	35.0	37.0	33.0	37.0
8	35.739548505428814	37.0	35.0	37.0	33.0	37.0
9	37.45226270045287	39.0	37.0	39.0	34.0	39.0
10-11	37.4247576566102	39.0	37.0	39.0	34.0	39.0
12-13	37.37560617686064	39.0	37.0	39.0	34.0	39.0
14-15	38.724253963695006	40.0	38.0	41.0	34.0	41.0
16-17	38.60961373273953	40.0	38.0	41.0	34.0	41.0
18-19	38.633686452858356	40.0	38.0	41.0	34.0	41.0
20-21	38.62204188966835	40.0	38.0	41.0	34.0	41.0
22-23	38.58232300824311	40.0	38.0	41.0	34.0	41.0
24-25	38.55739968168665	40.0	38.0	41.0	34.0	41.0
26-27	38.44849622290958	40.0	38.0	41.0	34.0	41.0
28-29	38.222725346422294	40.0	38.0	41.0	33.5	41.0
30-31	37.72001566823608	39.5	36.5	41.0	33.0	41.0
32-33	37.345759327258	39.0	35.5	41.0	33.0	41.0
34-35	37.11405822111789	39.0	35.0	40.5	32.0	41.0
36-37	37.26341388037366	39.0	35.0	41.0	33.0	41.0
38-39	37.13156539481214	39.0	35.0	41.0	31.5	41.0
40-41	36.49713822421321	39.0	35.0	41.0	29.5	41.0
42-43	36.49276420529731	39.0	35.0	41.0	29.5	41.0
44-45	36.413345303034454	39.0	35.0	41.0	29.0	41.0
46-47	36.420162889980546	39.0	35.0	41.0	29.0	41.0
48-49	36.301471574607994	39.0	35.0	41.0	28.5	41.0
50-51	36.025161054863574	38.5	35.0	40.5	28.0	41.0
52-53	35.78719732395552	38.0	35.0	40.0	28.0	41.0
54-55	35.5045557112649	38.0	35.0	40.0	27.5	41.0
56-57	35.11105853865499	38.0	34.0	40.0	26.0	41.0
58-59	34.793203299183745	37.5	34.0	40.0	26.0	41.0
60-61	34.62757064957805	37.0	34.0	40.0	26.0	41.0
62-63	34.3027094598643	37.0	34.0	40.0	25.5	41.0
64-65	33.94039369020557	36.0	34.0	39.0	25.0	41.0
66-67	33.466468422282134	35.5	34.0	39.0	24.5	41.0
68-69	33.00924635760208	35.0	33.0	38.5	22.5	40.5
70-71	32.53773136361684	35.0	33.0	37.5	21.5	40.0
72-73	32.15901300786226	35.0	33.0	37.0	20.0	39.0
74-75	31.783623515501947	35.0	32.0	36.5	20.0	39.0
76-77	31.35324916376642	35.0	32.0	36.0	18.5	38.5
78-79	31.066187805847775	35.0	32.0	35.5	17.5	37.0
80-81	30.806983171989394	35.0	32.0	35.0	15.5	37.0
82-83	30.534122138497114	35.0	31.5	35.0	10.5	36.0
84-85	30.237957059960657	35.0	31.0	35.0	7.0	36.0
86-87	29.982057698600777	35.0	31.0	35.0	4.5	36.0
88-89	29.720370926507265	35.0	31.0	35.0	2.0	35.0
90-91	29.527651925975285	34.0	31.0	35.0	2.0	35.0
92-93	29.343018841423817	34.0	31.0	35.0	2.0	35.0
94-95	29.06079943905823	34.0	30.0	35.0	2.0	35.0
96-97	28.752130639952476	34.0	30.0	35.0	2.0	35.0
98-99	28.414691864088304	34.0	30.0	35.0	2.0	35.0
100-101	27.212525831727657	33.0	27.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.36546895358654297
1101	2	0.34483237278892176
1101	3	0.36544572410086573
1101	4	0.35204270693497364
1101	5	0.3670594615524365
1101	6	0.34700378099081775
1101	7	0.3779632285155543
1101	8	0.31757345142184334
1101	9	0.3943909184537091
1101	10-11	0.33132433756123447
1101	12-13	0.3915133168711975
1101	14-15	0.5311734909558439
1101	16-17	0.4354060463573646
1101	18-19	0.4762268201663602
1101	20-21	0.478357534965248
1101	22-23	0.4694498769233988
1101	24-25	0.4580634456608621
1101	26-27	0.5213285234560558
1101	28-29	0.6263403910080925
1101	30-31	0.6764481246670826
1101	32-33	0.6606937875522689
1101	34-35	0.7170349081209366
1101	36-37	0.6789387449637942
1101	38-39	0.721566818962728
1101	40-41	0.6756045839209648
1101	42-43	0.6376389341165662
1101	44-45	0.6648922536380866
1101	46-47	0.6541433680919937
1101	48-49	0.6375174898549503
1101	50-51	0.5866333458524622
1101	52-53	0.5113526955630476
1101	54-55	0.5250381156348638
1101	56-57	0.5143378081965082
1101	58-59	0.46377903807426435
1101	60-61	0.4703948522967565
1101	62-63	0.4634766325889146
1101	64-65	0.44409780010369104
1101	66-67	0.41163014113489993
1101	68-69	0.40524352286103493
1101	70-71	0.4884365685690284
1101	72-73	0.5261723357374279
1101	74-75	0.43851411284230934
1101	76-77	0.32300587019486215
1101	78-79	0.24728308214474026
1101	80-81	0.2865794573949003
1101	82-83	0.2906816328673507
1101	84-85	0.31572214384388175
1101	86-87	0.3420925558990042
1101	88-89	0.4335299798553116
1101	90-91	0.4181342757667075
1101	92-93	0.3943869598529197
1101	94-95	0.4886555102875043
1101	96-97	0.45505748641697963
1101	98-99	0.4792856144626434
1101	100-101	0.6732168901212923
1102	1	0.3394279595394778
1102	2	0.3559044532724229
1102	3	0.3582193122994468
1102	4	0.27883431454730356
1102	5	0.278928614215495
1102	6	0.31809769798682197
1102	7	0.34295653484205246
1102	8	0.2752643452355059
1102	9	0.3969654640282698
1102	10-11	0.34141426902663596
1102	12-13	0.3667770086872366
1102	14-15	0.5353437183356675
1102	16-17	0.490663252572638
1102	18-19	0.538359203600173
1102	20-21	0.44312304658075874
1102	22-23	0.42468869705930956
1102	24-25	0.4757535383598963
1102	26-27	0.5711331406159772
1102	28-29	0.5561347893680519
1102	30-31	0.5605663636548783
1102	32-33	0.5634556522315037
1102	34-35	0.6391671382710697
1102	36-37	0.6473002233044696
1102	38-39	0.5813557036778718
1102	40-41	0.5181592461137328
1102	42-43	0.5518788128359091
1102	44-45	0.5485918077782799
1102	46-47	0.5094595751131763
1102	48-49	0.548337106090699
1102	50-51	0.44329315468793595
1102	52-53	0.4100802537465924
1102	54-55	0.4353142767091569
1102	56-57	0.4038816628383586
1102	58-59	0.41329815621727306
1102	60-61	0.3604780534438703
1102	62-63	0.47027579862012914
1102	64-65	0.37973563784460396
1102	66-67	0.3439866271042078
1102	68-69	0.2983954418215973
1102	70-71	0.399910797868408
1102	72-73	0.4394983370155856
1102	74-75	0.4640465595917469
1102	76-77	0.29906417318710155
1102	78-79	0.2025928305511222
1102	80-81	0.28514303892085735
1102	82-83	0.3062423605387359
1102	84-85	0.2357253127680714
1102	86-87	0.26168607860019577
1102	88-89	0.3655389376225706
1102	90-91	0.4269731769409866
1102	92-93	0.31523616415413613
1102	94-95	0.39877444245442106
1102	96-97	0.39377581690649777
1102	98-99	0.4207516658809851
1102	100-101	0.5533026663754903
1103	1	0.2507030069190037
1103	2	0.2423470905273888
1103	3	0.27254692963391847
1103	4	0.231774917327769
1103	5	0.25315402678995014
1103	6	0.2797843361892447
1103	7	0.31255811372840725
1103	8	0.2746821740220682
1103	9	0.3522951341016167
1103	10-11	0.2961891864430868
1103	12-13	0.34827001114592804
1103	14-15	0.4344402174833135
1103	16-17	0.48802451357626353
1103	18-19	0.45778860891500983
1103	20-21	0.4181800380090053
1103	22-23	0.4494723435231194
1103	24-25	0.44940311723640036
1103	26-27	0.4751806036777211
1103	28-29	0.45260667689758094
1103	30-31	0.49708663987549073
1103	32-33	0.4723682146747379
1103	34-35	0.5083163241775992
1103	36-37	0.4660992130692989
1103	38-39	0.3872684119719878
1103	40-41	0.43518197992602126
1103	42-43	0.4090308618910967
1103	44-45	0.3972758799030842
1103	46-47	0.4357501331217293
1103	48-49	0.4177867518455898
1103	50-51	0.3809506188420215
1103	52-53	0.3834813932142751
1103	54-55	0.38879343627362317
1103	56-57	0.4183929008816918
1103	58-59	0.41527559307779427
1103	60-61	0.3244374831343748
1103	62-63	0.439104508691905
1103	64-65	0.43378007738333935
1103	66-67	0.3488967492518995
1103	68-69	0.39250257347177353
1103	70-71	0.41204903246016755
1103	72-73	0.41270701846116253
1103	74-75	0.38283349927506194
1103	76-77	0.4435346601257031
1103	78-79	0.3765347009227007
1103	80-81	0.366113249165295
1103	82-83	0.46522778240469265
1103	84-85	0.46857553377572003
1103	86-87	0.5141531985576577
1103	88-89	0.5652186026215027
1103	90-91	0.5550222810671599
1103	92-93	0.504387499534662
1103	94-95	0.5356427715042109
1103	96-97	0.5479135235480079
1103	98-99	0.5524418486910214
1103	100-101	0.6784491003919619
1104	1	0.2967514162150877
1104	2	0.269242639424526
1104	3	0.18908096904065985
1104	4	0.19815423390693354
1104	5	0.17548969523703306
1104	6	0.2000844043069847
1104	7	0.2539388574412058
1104	8	0.21416261044338114
1104	9	0.23428186491312886
1104	10-11	0.21613020158670082
1104	12-13	0.2771645107344085
1104	14-15	0.33456622261238067
1104	16-17	0.4184063210613971
1104	18-19	0.3736106201932827
1104	20-21	0.4158484055420004
1104	22-23	0.4119360920207882
1104	24-25	0.3736063592993446
1104	26-27	0.40934923332631
1104	28-29	0.3699473391583652
1104	30-31	0.46660503112737217
1104	32-33	0.41684516135041605
1104	34-35	0.47967249811156876
1104	36-37	0.46197553736083563
1104	38-39	0.40266037518624387
1104	40-41	0.42270532760147006
1104	42-43	0.36699425025869914
1104	44-45	0.3733815411075554
1104	46-47	0.35675295107594707
1104	48-49	0.3178991953584145
1104	50-51	0.2529789727176137
1104	52-53	0.3216807310088754
1104	54-55	0.3294509713622631
1104	56-57	0.30953401308578066
1104	58-59	0.33763071247155807
1104	60-61	0.2652160495413227
1104	62-63	0.3579901777467711
1104	64-65	0.322334129050347
1104	66-67	0.4424024680187131
1104	68-69	0.38435353656002036
1104	70-71	0.4029918365600338
1104	72-73	0.43209888142986586
1104	74-75	0.43466827959111143
1104	76-77	0.5462054426303808
1104	78-79	0.42830241942148106
1104	80-81	0.49196457669192384
1104	82-83	0.4965498997755482
1104	84-85	0.48578350915239454
1104	86-87	0.47940880703897193
1104	88-89	0.4969778056331471
1104	90-91	0.6229582061521093
1104	92-93	0.5543060413626044
1104	94-95	0.6595433400650172
1104	96-97	0.6642885779598835
1104	98-99	0.6466954835734597
1104	100-101	0.7372662743411631
1105	1	0.047920441938060776
1105	2	0.08277436160074814
1105	3	0.10718287708052543
1105	4	0.00894922181023361
1105	5	0.10482793488657194
1105	6	0.07758114750277656
1105	7	0.10546931386430458
1105	8	0.1074781169483785
1105	9	0.11292125172232659
1105	10-11	0.056056804289362105
1105	12-13	0.1568417426377664
1105	14-15	0.24235053605368506
1105	16-17	0.09257549990983449
1105	18-19	0.16498997182353037
1105	20-21	0.09760365392241965
1105	22-23	0.06133408735854573
1105	24-25	0.11244037823144026
1105	26-27	0.15812255975324518
1105	28-29	0.2543682518533288
1105	30-31	0.262886088394616
1105	32-33	0.12836649746142825
1105	34-35	0.23565999769672175
1105	36-37	0.19110875378109426
1105	38-39	0.2924236965762148
1105	40-41	0.21038785462107512
1105	42-43	0.13021467509467755
1105	44-45	0.05828727232679398
1105	46-47	0.14887386893811083
1105	48-49	0.17236628934804088
1105	50-51	0.17856376914477323
1105	52-53	0.07851814516725142
1105	54-55	0.07022137622261937
1105	56-57	0.13929562678028162
1105	58-59	0.12121665555425665
1105	60-61	-0.01842081458367062
1105	62-63	0.11065448199634886
1105	64-65	0.007433735167133193
1105	66-67	-0.024628086917196867
1105	68-69	0.046743161249303
1105	70-71	0.061532178082323696
1105	72-73	0.13051670577962682
1105	74-75	0.08088613847287363
1105	76-77	0.2458862422099486
1105	78-79	0.20414038747812313
1105	80-81	0.14184019113671553
1105	82-83	0.1868024139527833
1105	84-85	0.1803129865387696
1105	86-87	0.21017233175602357
1105	88-89	0.22429023639586276
1105	90-91	0.31351628334162385
1105	92-93	0.2250171316368892
1105	94-95	0.35648731084977214
1105	96-97	0.31340074435538057
1105	98-99	0.36286316393487894
1105	100-101	0.43665107337292497
1106	1	-0.14302500514484606
1106	2	-0.2119419417759687
1106	3	-0.19978805888128193
1106	4	-0.307235931062543
1106	5	-0.12287483561698309
1106	6	-0.09447820817695174
1106	7	-0.12680684869091152
1106	8	-0.1386915808783371
1106	9	-0.150046811957246
1106	10-11	-0.13481838742043806
1106	12-13	-0.14547255889395672
1106	14-15	-0.2811935034813615
1106	16-17	-0.24633957315282373
1106	18-19	-0.28336509775441243
1106	20-21	-0.3894243478466137
1106	22-23	-0.2344647805643305
1106	24-25	-0.1382802694842411
1106	26-27	-0.4764333525204094
1106	28-29	-0.32126712911610156
1106	30-31	-0.24703885720101937
1106	32-33	-0.32135817176338577
1106	34-35	-0.1919949301764916
1106	36-37	-0.3938769826362716
1106	38-39	-0.23388972098275929
1106	40-41	-0.2044723169857221
1106	42-43	-0.19675013202594727
1106	44-45	-0.29301699745479937
1106	46-47	-0.19532015743968145
1106	48-49	-0.10887155754507916
1106	50-51	-0.26276931390361113
1106	52-53	-0.22417162602414464
1106	54-55	-0.17410228368366631
1106	56-57	-0.12637579922404285
1106	58-59	-0.07832593416668487
1106	60-61	-0.10398665811364083
1106	62-63	-0.005501475529236188
1106	64-65	-0.12603368633801182
1106	66-67	-0.1567301994245227
1106	68-69	-0.053201748331495935
1106	70-71	0.031667607599004555
1106	72-73	0.011233517573494112
1106	74-75	0.014223369801158015
1106	76-77	0.09285032530903692
1106	78-79	-0.12830439700244867
1106	80-81	-0.10805458673936741
1106	82-83	-0.0318408709893383
1106	84-85	-0.08812525271249783
1106	86-87	-0.017408204768113222
1106	88-89	0.04210301625748869
1106	90-91	0.07247166240614789
1106	92-93	-0.07401266493423364
1106	94-95	-0.02585151921280371
1106	96-97	-0.12452522399350485
1106	98-99	-0.05570602618775311
1106	100-101	0.053563864398686434
1107	1	-0.2568750709927521
1107	2	-0.31690690793881515
1107	3	-0.32140679134079875
1107	4	-0.2096532617495157
1107	5	-0.3224882491545813
1107	6	-0.22985392427522555
1107	7	-0.2517761072786797
1107	8	-0.2312568634591372
1107	9	-0.20849441235285582
1107	10-11	-0.24503200714283224
1107	12-13	-0.18154434684714715
1107	14-15	-0.23410388742245658
1107	16-17	-0.3162467482490925
1107	18-19	-0.3422563090076238
1107	20-21	-0.2901949344389507
1107	22-23	-0.28643596173004937
1107	24-25	-0.37809155161035335
1107	26-27	-0.27054770564239305
1107	28-29	-0.33932767809901065
1107	30-31	-0.19383510630350997
1107	32-33	-0.2583280411600768
1107	34-35	-0.4334155436732985
1107	36-37	-0.3396059732400545
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2308	36-37	-1.2952466018547995
2308	38-39	-0.8337435571034248
2308	40-41	-0.8992152239793754
2308	42-43	-0.7161542836528554
2308	44-45	-0.8882969817812736
2308	46-47	-1.141653096029529
2308	48-49	-1.8750958785778167
2308	50-51	-2.8187976413214955
2308	52-53	-1.9589549876457042
2308	54-55	-1.4284496746896878
2308	56-57	-0.6740941720005296
2308	58-59	-0.7441234753823665
2308	60-61	-0.24019831935203229
2308	62-63	-0.22822080538327327
2308	64-65	-0.2854166317976521
2308	66-67	-0.28286966325298835
2308	68-69	-0.6798099875183397
2308	70-71	-0.8890964041091323
2308	72-73	-0.9764378351971637
2308	74-75	-1.0290720446532085
2308	76-77	-0.8107081075847624
2308	78-79	-0.46787636195398363
2308	80-81	-0.653756903547432
2308	82-83	-0.5600991544020957
2308	84-85	-0.9621312384156475
2308	86-87	-0.7257948623187573
2308	88-89	-0.7934848504569736
2308	90-91	-1.1817883472908264
2308	92-93	-0.8475628417034748
2308	94-95	-1.8216627676284212
2308	96-97	-1.162321839051497
2308	98-99	-1.6006529395345872
2308	100-101	-1.8604488149396268
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	127.0
11	946.0
12	2641.0
13	5331.0
14	8692.0
15	12761.0
16	17821.0
17	27791.0
18	55207.0
19	136171.0
20	146298.0
21	99875.0
22	87461.0
23	83225.0
24	87726.0
25	96035.0
26	108315.0
27	121229.0
28	134692.0
29	149606.0
30	169883.0
31	197957.0
32	242540.0
33	309961.0
34	436546.0
35	675180.0
36	1141700.0
37	1926599.0
38	1552787.0
39	209493.0
40	110.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	28.05944808705125	32.201645516529034	19.157517563391586	20.58138883302813
2	23.21802620978844	25.130987084318107	32.7655952801713	18.885391425722155
3	22.64513737663902	27.882886303041005	22.488770369737864	26.983205950582107
4	28.650008866295533	22.62496685752045	27.43294909484947	21.292075181334543
5	22.772879954724885	29.15959647317927	21.749884107450285	26.317639464645552
6	22.697049476354888	22.419477419813393	26.07040202525111	28.813071078580606
7	28.527457498181256	22.49546557512178	21.844890527327475	27.132186399369484
8	32.92856046049429	22.44191606104572	22.052975570020326	22.57654790843967
9	22.131486556342946	27.086775441113364	22.200221572485425	28.58151643005827
10-11	30.38511015432206	22.407299908571634	22.061756962589087	25.145832974517223
12-13	27.720110803230025	22.223886590006156	22.25750816690784	27.798494439855975
14-15	27.541909923774117	22.212732631096852	22.411223638538473	27.834133806590554
16-17	27.661792912931038	25.151260699896394	22.26959336087909	24.91735302629348
18-19	24.71318564906984	22.249471357741562	25.268790663972734	27.76855232921586
20-21	24.517896696377043	27.645837219665566	22.6099147744019	25.22635130955549
22-23	21.86986412856929	25.044828766483608	28.243639009080496	24.84166809586661
24-25	21.937337729204657	22.180414923224674	31.092121417064476	24.790125930506193
26-27	21.86816720218235	22.21099952563471	33.890434595505646	22.030398676677297
28-29	21.923304481688007	22.170275083186713	33.98608149277852	21.920338942346763
30-31	21.909905921416716	22.027991600397236	33.97023750862324	22.09186496956281
32-33	21.873408727071418	22.04164850235063	34.02360160238985	22.061341168188104
34-35	21.96676942513478	22.202087772615595	33.886921534836965	21.94422126741266
36-37	21.947736210575673	22.091085262150404	33.84034031648259	22.120838210791334
38-39	22.017093343239004	22.179420973231952	33.473399489049434	22.330086194479613
40-41	22.770735412406086	22.901435799946597	31.156665377108915	23.1711634105384
42-43	22.923735384443336	22.97009349795922	31.24465288210223	22.861518235495215
44-45	22.93849406323116	23.336953282280447	30.94378291659849	22.7807697378899
46-47	22.802245035971772	23.787782278305063	30.725386630832258	22.684586054890907
48-49	22.778633448282324	23.930595658056237	30.42782238200758	22.86294851165386
50-51	22.72996020900597	24.124315820326963	30.27358938952109	22.87213458114598
52-53	22.765292995619156	24.220294774981607	30.119348280301377	22.895063949097864
54-55	22.946255163574207	24.001436082357625	29.85644937733883	23.19585937672934
56-57	22.94884741796736	23.934637572279716	29.78322089350427	23.333294116248656
58-59	23.06898790780524	24.02793746793773	29.566805869250402	23.336268755006625
60-61	23.237092591326903	23.988913058758254	29.207245737222827	23.566748612692017
62-63	23.36479311694074	24.093800312588463	28.8940745734293	23.647331997041494
64-65	23.51587188190822	24.294583700134364	28.443973623801742	23.745570794155668
66-67	23.625221270951112	24.304985281755872	28.03942096373555	24.030372483557468
68-69	23.678835112935648	24.508058063861903	27.819230670326306	23.993876152876144
70-71	23.86725619652564	24.72741279274457	27.524853392322708	23.88047761840708
72-73	24.0387625567791	24.668574050594756	27.260826706350734	24.031836686275412
74-75	23.990944158779246	24.76546966259835	27.234199203641502	24.009386974980902
76-77	24.07180498756494	24.885689402765195	27.037067863719784	24.005437745950083
78-79	24.163189964038086	24.752840347974672	26.967648840847975	24.116320847139264
80-81	24.15064093812931	24.769808953293154	27.008035162253485	24.07151494632405
82-83	24.234774464805607	24.88536043003431	26.85267994271918	24.027185162440905
84-85	24.27919696519817	24.80209528051249	26.742541564054346	24.176166190234994
86-87	24.232107080365825	24.870721034222022	26.750151069054628	24.14702081635752
88-89	24.38330968285884	24.95427487329843	26.575890004006368	24.086525439836358
90-91	24.46429260182231	24.833875216411595	26.482381542774235	24.21945063899186
92-93	24.38686405395948	24.91738637101024	26.449515173678133	24.24623440135214
94-95	24.476674440751363	25.012024390698002	26.27680653772687	24.234494630823765
96-97	24.500079449698635	24.90555189642185	26.173890524380138	24.420478129499372
98-99	24.417640666767824	25.013454246283846	26.188785908464553	24.380119178483785
100-101	24.569241713317822	25.239277815276594	25.72945908919725	24.462021382208338
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6233.0
1	3749.5
2	972.5
3	604.0
4	476.0
5	411.5
6	385.0
7	381.0
8	379.0
9	379.0
10	413.5
11	423.5
12	629.0
13	1121.5
14	1682.0
15	2709.0
16	59052.0
17	72993.0
18	28858.5
19	22620.5
20	16972.5
21	14843.0
22	15057.0
23	16286.5
24	18100.5
25	20758.0
26	24177.0
27	28332.0
28	33584.5
29	40038.5
30	47498.0
31	55359.0
32	64456.0
33	75796.5
34	87422.5
35	99177.5
36	115401.0
37	132257.5
38	148318.5
39	165346.5
40	180916.0
41	193841.0
42	208167.0
43	225076.5
44	243613.5
45	265295.5
46	286566.5
47	309235.5
48	331356.0
49	345808.5
50	350224.5
51	353655.5
52	361208.0
53	366739.5
54	364766.0
55	353325.0
56	331400.0
57	301651.5
58	271690.5
59	242019.5
60	210259.0
61	177784.5
62	144937.0
63	110668.0
64	80113.0
65	56699.5
66	39589.5
67	28962.5
68	21634.0
69	16201.5
70	12446.0
71	9518.0
72	7698.0
73	5863.5
74	4350.0
75	3643.0
76	2886.0
77	1832.0
78	1037.5
79	615.0
80	345.0
81	214.0
82	134.0
83	89.5
84	51.5
85	20.5
86	7.5
87	2.0
88	2.0
89	0.5
90	0.5
91	1.5
92	1.5
93	1.0
94	1.0
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	6.064497630358196E-6
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	6.0644976303581955E-5
28-29	0.0
30-31	0.003256635227502351
32-33	0.006307077535572524
34-35	0.001576769383893131
36-37	0.0017587043128038768
38-39	0.003256635227502351
40-41	0.003365796184848799
42-43	0.002359089578209338
44-45	0.0069741722749119255
46-47	0.0014130279478734597
48-49	4.184503364947155E-4
50-51	0.003062571303330889
52-53	0.0021832191469289505
54-55	6.48901246448327E-4
56-57	0.0
58-59	9.096746445537294E-5
60-61	6.246432559268942E-4
62-63	0.0
64-65	0.0
66-67	7.883846919465655E-5
68-69	0.006573915431308285
70-71	0.006179723085335002
72-73	0.0030928937914826795
74-75	0.0036811500616274247
76-77	0.03260273926080566
78-79	0.05747324404290462
80-81	0.0491345598011621
82-83	7.459332085340581E-4
84-85	9.94577611378744E-4
86-87	0.001601027374414564
88-89	0.004038955421818558
90-91	0.0
92-93	1.2128995260716392E-5
94-95	6.670947393394016E-5
96-97	0.006015981649315331
98-99	0.00117651254028949
100-101	1.2128995260716392E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	8244706.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.25525699081635
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.52606965130762	42.60165721080272
2	9.741299386416582	10.1806820572336
3	3.0019361611374147	4.706008367107808
4	1.3604894547045772	2.8437090435553305
5	0.7813080828121839	2.0413727328176345
6	0.521258928998484	1.6343111576144085
7	0.3671358837872371	1.342934597049679
8	0.2897746338485029	1.2113798368938582
9	0.24546203120001117	1.1544013369659927
>10	2.0317881888774725	20.89605297168132
>50	0.12051912646691267	4.049784356269878
>100	0.011347154219812252	0.9498637686663156
>500	7.473468737797253E-4	0.27813163708081134
>1k	6.771651696231266E-4	0.8161590611828634
>5k	1.1675261243865007E-4	0.34639904455351045
>10k+	7.005156746319004E-5	4.947152820524301
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	329591	3.9976076769747766	No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT	60023	0.72801868253398	No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15828	0.19197773698661905	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.6386985782149175E-5	0.0	0.0	1.2128995260716392E-5	0.0
2	3.6386985782149175E-5	0.0	0.0	1.2128995260716392E-5	0.0
3	4.851598104286557E-5	0.0	0.0	1.2128995260716392E-5	1.2128995260716392E-5
4	4.851598104286557E-5	0.0	0.0	2.4257990521432784E-5	2.4257990521432784E-5
5	4.851598104286557E-5	0.0	0.0	2.4257990521432784E-5	8.490296682501474E-5
6	6.064497630358196E-5	0.0	0.0	3.6386985782149175E-5	8.490296682501474E-5
7	7.277397156429835E-5	1.2128995260716392E-5	0.0	3.6386985782149175E-5	9.703196208573114E-5
8	7.277397156429835E-5	1.2128995260716392E-5	0.0	3.6386985782149175E-5	1.334189478678803E-4
9	8.490296682501474E-5	1.2128995260716392E-5	0.0	3.6386985782149175E-5	1.9406392417146227E-4
10-11	9.703196208573112E-5	2.4257990521432784E-5	0.0	4.851598104286557E-5	2.48644402844686E-4
12-13	1.1522545497680572E-4	2.4257990521432784E-5	0.0	6.670947393394016E-5	2.910958862571934E-4
14-15	1.273544502375221E-4	2.4257990521432784E-5	0.0	9.096746445537294E-5	3.2141837440898436E-4
16-17	1.6374143601967129E-4	2.4257990521432784E-5	0.0	9.703196208573114E-5	3.517408625607753E-4
18-19	2.0012842180182046E-4	2.4257990521432784E-5	0.0	9.703196208573114E-5	3.820633507125664E-4
20-21	2.3651540758396963E-4	2.4257990521432784E-5	0.0	9.703196208573114E-5	4.0632134123399913E-4
22-23	2.547089004750442E-4	2.4257990521432784E-5	0.0	9.703196208573114E-5	4.487728246465065E-4
24-25	2.729023933661188E-4	2.4257990521432784E-5	0.0	9.703196208573114E-5	4.7303081516793927E-4
26-27	3.2748287203934257E-4	3.6386985782149175E-5	0.0	9.703196208573114E-5	4.912243080590138E-4
28-29	3.335473696697008E-4	3.6386985782149175E-5	0.0	1.0916095734644752E-4	5.154822985804467E-4
30-31	3.6993435545184996E-4	3.6386985782149175E-5	0.0	1.0916095734644752E-4	5.639982796233122E-4
32-33	4.245148341250737E-4	3.6386985782149175E-5	0.0	1.0916095734644752E-4	5.88256270144745E-4
34-35	4.609018199072229E-4	3.6386985782149175E-5	0.0	1.0916095734644752E-4	6.18578758296536E-4
36-37	4.790953127982975E-4	3.6386985782149175E-5	0.0	1.1522545497680572E-4	6.428367488179687E-4
38-39	5.154822985804467E-4	3.6386985782149175E-5	0.0	1.273544502375221E-4	6.670947393394016E-4
40-41	5.579337819929539E-4	3.6386985782149175E-5	0.0	1.334189478678803E-4	7.034817251215506E-4
42-43	6.064497630358196E-4	3.6386985782149175E-5	0.0	1.455479431285967E-4	7.580622037947746E-4
44-45	6.367722511876105E-4	3.6386985782149175E-5	0.0	1.7587043128038766E-4	8.308361753590729E-4
46-47	6.852882322304762E-4	3.6386985782149175E-5	0.0	1.8193492891074587E-4	9.27868137444804E-4
48-49	7.338042132733417E-4	3.6386985782149175E-5	0.0	1.8193492891074587E-4	0.0010430935924216097
50-51	8.793521564019384E-4	3.6386985782149175E-5	0.0	1.8193492891074587E-4	0.0010673515829430424
52-53	0.00117651254028949	3.6386985782149175E-5	0.0	1.8193492891074587E-4	0.0010976740710948335
54-55	0.00154644689574134	3.6386985782149175E-5	0.0	1.8193492891074587E-4	0.0011340610568769827
56-57	0.002177154649298592	4.2451483412507375E-5	0.0	1.8193492891074587E-4	0.0012007705308109229
58-59	0.0030140553222880234	4.851598104286557E-5	0.0	1.8193492891074587E-4	0.0012432220142234301
60-61	0.004111729393382857	4.851598104286557E-5	0.0	1.8193492891074587E-4	0.0012614155071145048
62-63	0.005549015331777749	4.851598104286557E-5	0.0	1.8193492891074587E-4	0.0013099314881573703
64-65	0.007768621464488848	4.851598104286557E-5	0.0	1.8193492891074587E-4	0.0013523829715698776
66-67	0.010600741857866126	4.851598104286557E-5	0.0	1.9406392417146227E-4	0.0014008989526127433
68-69	0.014306149910014984	4.851598104286557E-5	0.0	1.9406392417146227E-4	0.0014433504360252506
70-71	0.019103167535628317	4.851598104286557E-5	0.0	1.9406392417146227E-4	0.0014979309146984743
72-73	0.025458761052243706	4.851598104286557E-5	0.0	1.9406392417146227E-4	0.0015646403886324146
74-75	0.03425228261626309	4.851598104286557E-5	0.0	1.9406392417146227E-4	0.0016434788578270712
76-77	0.045750570123422235	4.851598104286557E-5	0.0	1.9406392417146227E-4	0.001673801345978862
78-79	0.060590395824908744	6.064497630358196E-5	0.0	2.0012842180182046E-4	0.0017162528293913695
80-81	0.07952982192451738	6.064497630358196E-5	0.0	2.0619291943217867E-4	0.0017587043128038768
82-83	0.10349065206206262	6.064497630358196E-5	0.0	2.0619291943217867E-4	0.001801155796216384
84-85	0.13474707284892876	6.064497630358196E-5	0.0	2.0619291943217867E-4	0.001855736274889608
86-87	0.1715888959533548	6.064497630358196E-5	1.2128995260716392E-5	2.0619291943217867E-4	0.0018981877583021153
88-89	0.2183461726834165	6.064497630358196E-5	1.2128995260716392E-5	2.0619291943217867E-4	0.0019224457488235481
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	47475	0.0	94.0951	1
TCGACGG	48580	0.0	91.92548	2
TATCTCG	44595	0.0	91.471	2
ATATCTC	45435	0.0	89.947136	1
ACGGCGC	50020	0.0	89.241104	5
CGACGGC	50255	0.0	88.89941	3
GACGGCG	50300	0.0	88.810425	4
CGGCGCG	50580	0.0	88.33757	6
ATCTCGA	47290	0.0	86.17783	3
TCTCGAG	47660	0.0	84.15358	4
CTCGAGG	49205	0.0	81.56913	5
TCGAGGG	49265	0.0	81.508354	6
CGAGGGC	50210	0.0	80.01212	7
AGGGCGC	50415	0.0	79.87518	9
GAGGGCG	51890	0.0	77.6413	8
CGCGCCG	99080	0.0	46.088253	9
GCGCGCC	100985	0.0	45.26116	8
GGCGCGC	100960	0.0	45.16418	7
CCATATT	55135	0.0	40.31303	18-19
TCCATAT	55180	0.0	40.267246	18-19
>>END_MODULE