FastQCFastQC Report
Tue 31 May 2016
SRR1051270_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051270_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8244706
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA486540.5901241354148953No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT186140.22576911778297493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG55150.090.3842851
TCGACGG57700.086.521472
ACGGCGC72450.069.0369645
CGACGGC77650.064.658843
CGGCGCG78950.063.292956
GACGGCG80400.062.3877874
ATCTCGA41050.056.2582241
CGCGCCG133150.043.9145669
TCTCGAG54350.042.737222
GCGCGCC153250.038.1548088
GGCGCGC154600.037.8216367
TCGAGGG64400.035.846124
CTCGAGG69000.033.8696253
GGGCGCG71800.032.2839858
AGGGCGC75800.030.6430077
CGAGGGC77300.030.1098335
TCCATAT101750.025.06868618-19
CCATATT103050.025.0520518-19
GAGGGCG99900.023.5359346
ATCCATA109850.023.2850616-17
Produced by FastQC (version 0.11.5)