##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051270_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8244706 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.240159321630145 33.0 31.0 34.0 31.0 34.0 2 32.51112471445313 34.0 31.0 34.0 31.0 34.0 3 32.642394040490956 34.0 31.0 34.0 31.0 34.0 4 36.17101701382681 37.0 35.0 37.0 35.0 37.0 5 36.079219440935795 37.0 35.0 37.0 35.0 37.0 6 36.08822243024797 37.0 35.0 37.0 35.0 37.0 7 36.057277845929256 37.0 35.0 37.0 35.0 37.0 8 36.06759743767698 37.0 35.0 37.0 35.0 37.0 9 37.84681454984568 39.0 38.0 39.0 35.0 39.0 10-11 37.81456840304554 39.0 38.0 39.0 35.0 39.0 12-13 37.78734930026613 39.0 38.0 39.0 35.0 39.0 14-15 39.16094370132786 41.0 39.0 41.0 36.0 41.0 16-17 39.11135315194987 40.0 38.5 41.0 36.0 41.0 18-19 39.053749217983025 40.0 38.5 41.0 35.5 41.0 20-21 39.01698665786263 40.0 38.5 41.0 35.0 41.0 22-23 38.916627287862056 40.0 38.0 41.0 35.0 41.0 24-25 38.824657604528284 40.0 38.0 41.0 35.0 41.0 26-27 38.657787676116044 40.0 38.0 41.0 34.5 41.0 28-29 38.45973258476409 40.0 38.0 41.0 34.0 41.0 30-31 38.28329779133422 40.0 38.0 41.0 34.0 41.0 32-33 38.44414603746938 40.0 38.0 41.0 34.0 41.0 34-35 38.40563338462282 40.0 38.0 41.0 34.0 41.0 36-37 38.33188739537832 40.0 38.0 41.0 34.0 41.0 38-39 38.22221890022519 40.0 38.0 41.0 33.5 41.0 40-41 38.018362631729985 40.0 37.5 41.0 33.0 41.0 42-43 37.904875928868776 40.0 37.0 41.0 33.0 41.0 44-45 37.81491383683057 40.0 37.0 41.0 33.0 41.0 46-47 37.67531740974148 40.0 37.0 41.0 32.5 41.0 48-49 37.56570822537517 40.0 36.0 41.0 32.0 41.0 50-51 37.57992261943603 40.0 36.0 41.0 32.0 41.0 52-53 37.417794157851105 39.0 36.0 41.0 32.0 41.0 54-55 37.097382490048766 39.0 35.0 41.0 31.0 41.0 56-57 36.85881697904085 39.0 35.0 41.0 31.0 41.0 58-59 36.580763704612394 38.0 35.0 40.0 31.0 41.0 60-61 36.27993393578861 38.0 35.0 40.0 31.0 41.0 62-63 35.90153760485819 37.0 35.0 40.0 30.0 41.0 64-65 35.49873251999526 36.5 34.0 39.0 30.0 41.0 66-67 35.07773709578001 36.0 34.0 39.0 29.0 41.0 68-69 34.67762301045058 35.5 34.0 39.0 29.0 40.0 70-71 34.24482716545623 35.0 34.0 37.5 29.0 40.0 72-73 33.85282525538206 35.0 33.0 37.0 29.0 39.0 74-75 33.501167051923986 35.0 33.0 36.5 28.0 39.0 76-77 32.930304549367804 35.0 32.5 36.0 27.0 37.5 78-79 32.84282580846424 35.0 33.0 35.5 27.0 37.0 80-81 32.61045087599243 35.0 33.0 35.0 27.0 37.0 82-83 32.32751313388252 35.0 33.0 35.0 26.0 36.0 84-85 32.10314673440145 35.0 32.0 35.0 26.0 36.0 86-87 31.808925509290447 35.0 32.0 35.0 25.5 36.0 88-89 31.63126332218517 35.0 32.0 35.0 25.0 35.0 90-91 31.39003646703715 34.0 32.0 35.0 25.0 35.0 92-93 31.112796623675848 34.0 31.5 35.0 24.0 35.0 94-95 30.818067072373474 34.0 31.0 35.0 23.0 35.0 96-97 30.554597034751755 34.0 31.0 35.0 21.5 35.0 98-99 30.155414153033476 34.0 31.0 35.0 18.0 35.0 100-101 28.41465195969389 33.0 28.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4199986541524936 1101 2 0.40866634593639617 1101 3 0.37664395803216877 1101 4 0.23586287920785765 1101 5 0.2668208617795287 1101 6 0.24201736968543486 1101 7 0.3053280375616083 1101 8 0.2597481323608548 1101 9 0.313115398829936 1101 10-11 0.30216292150975477 1101 12-13 0.329809941105232 1101 14-15 0.4569291058245355 1101 16-17 0.3827681928679354 1101 18-19 0.3681009854540065 1101 20-21 0.39790143429675595 1101 22-23 0.3894106404563473 1101 24-25 0.3802243364269984 1101 26-27 0.4103961757507335 1101 28-29 0.4406507893377736 1101 30-31 0.4384068213485577 1101 32-33 0.3885109568026266 1101 34-35 0.37610081802256445 1101 36-37 0.35733013808832936 1101 38-39 0.35376508419169994 1101 40-41 0.421532251145301 1101 42-43 0.37909591956020705 1101 44-45 0.32261095407617546 1101 46-47 0.2798989006872361 1101 48-49 0.33728616819228563 1101 50-51 0.4254820626868039 1101 52-53 0.4044144265302876 1101 54-55 0.45896162881017233 1101 56-57 0.38045307707650977 1101 58-59 0.3994679034219857 1101 60-61 0.27132490425699984 1101 62-63 0.33561954162389185 1101 64-65 0.3263783139183545 1101 66-67 0.26524019811910193 1101 68-69 0.3004185950583391 1101 70-71 0.30081315240790474 1101 72-73 0.22658759021079078 1101 74-75 0.22110921180519938 1101 76-77 0.16077304222742583 1101 78-79 0.19862346806697673 1101 80-81 0.17243951533708213 1101 82-83 0.03541404222453082 1101 84-85 0.03639868528099299 1101 86-87 0.21454873749852155 1101 88-89 0.20717359287719006 1101 90-91 0.06371068238297894 1101 92-93 -0.06407551727522431 1101 94-95 0.03231506098331849 1101 96-97 0.09585380609875571 1101 98-99 0.1729972320687665 1101 100-101 0.3114307788256667 1102 1 0.3795468198034371 1102 2 0.36846881247355157 1102 3 0.2835912548429107 1102 4 0.18918804609985784 1102 5 0.2281011916292357 1102 6 0.22586005507664453 1102 7 0.2355732504187671 1102 8 0.20487397191006806 1102 9 0.2787881566286572 1102 10-11 0.28248229697322813 1102 12-13 0.26104283217157587 1102 14-15 0.3695215789264452 1102 16-17 0.36845274420705465 1102 18-19 0.36778577127775947 1102 20-21 0.32228623049544325 1102 22-23 0.34483977874359084 1102 24-25 0.33892035923899755 1102 26-27 0.35358280080887994 1102 28-29 0.4166284005012031 1102 30-31 0.35272624373577344 1102 32-33 0.31902988242753594 1102 34-35 0.3979355244746614 1102 36-37 0.43382143058873623 1102 38-39 0.40577117633363713 1102 40-41 0.44708506098990597 1102 42-43 0.46794267038396953 1102 44-45 0.42904358313722923 1102 46-47 0.33327307896595215 1102 48-49 0.36588359494655975 1102 50-51 0.47014059109655904 1102 52-53 0.4856585898971062 1102 54-55 0.5520865341418926 1102 56-57 0.4350792180032954 1102 58-59 0.46686797171577155 1102 60-61 0.4024415750371446 1102 62-63 0.45633483390356844 1102 64-65 0.4559832611142127 1102 66-67 0.41916647293695775 1102 68-69 0.4012645600371698 1102 70-71 0.45297875385569597 1102 72-73 0.43340516201609347 1102 74-75 0.4359955746925408 1102 76-77 0.4174871280665329 1102 78-79 0.44190804550036233 1102 80-81 0.4643071127868126 1102 82-83 0.342286600881863 1102 84-85 0.33486241074736967 1102 86-87 0.5278023954187994 1102 88-89 0.5598298210931674 1102 90-91 0.5198722404940206 1102 92-93 0.3935857443484032 1102 94-95 0.4881456973222278 1102 96-97 0.5401839386904328 1102 98-99 0.6022407457441155 1102 100-101 0.731821307498592 1103 1 0.27402683502051417 1103 2 0.27643241999508916 1103 3 0.2481061853692239 1103 4 0.14217785264632 1103 5 0.1572659889488932 1103 6 0.15959208855034035 1103 7 0.15698462567810623 1103 8 0.1508758754532593 1103 9 0.13576433962934686 1103 10-11 0.11374198644025313 1103 12-13 0.17811045671850678 1103 14-15 0.24447808552595518 1103 16-17 0.2388740103035687 1103 18-19 0.21902645876087234 1103 20-21 0.22662697969011703 1103 22-23 0.1733062962849914 1103 24-25 0.13794634472485967 1103 26-27 0.22121534004272547 1103 28-29 0.22235186354146919 1103 30-31 0.21238887314101618 1103 32-33 0.15634630022788798 1103 34-35 0.19726927555589668 1103 36-37 0.2406871427727566 1103 38-39 0.20092053881038652 1103 40-41 0.1941378302245056 1103 42-43 0.26766133750940213 1103 44-45 0.18430132240990815 1103 46-47 0.20227821842694027 1103 48-49 0.29589731126652197 1103 50-51 0.3054444956486435 1103 52-53 0.31867777610906955 1103 54-55 0.39383296328199435 1103 56-57 0.3649208091633085 1103 58-59 0.2891629814768635 1103 60-61 0.26403974328391655 1103 62-63 0.27174606956153013 1103 64-65 0.2735505283396833 1103 66-67 0.28317619785446624 1103 68-69 0.21798070773613887 1103 70-71 0.25885268887802937 1103 72-73 0.3368581309176406 1103 74-75 0.32914180696477047 1103 76-77 0.28279736397261956 1103 78-79 0.33263876015468696 1103 80-81 0.31838185797247576 1103 82-83 0.2228763665513469 1103 84-85 0.29930010280379094 1103 86-87 0.5103924922234633 1103 88-89 0.4504300885539294 1103 90-91 0.24531324575072588 1103 92-93 0.18418595899653312 1103 94-95 0.3276726757295201 1103 96-97 0.4012115104916205 1103 98-99 0.48105235420642245 1103 100-101 0.6161847555611999 1104 1 0.4031075447744641 1104 2 0.40888849312957376 1104 3 0.31938503250989925 1104 4 0.20183817791584602 1104 5 0.22815828290426055 1104 6 0.20050315912214245 1104 7 0.22659239550137755 1104 8 0.22624026382994344 1104 9 0.25234008903606764 1104 10-11 0.26365694120050165 1104 12-13 0.2612084053235364 1104 14-15 0.36690171502760194 1104 16-17 0.30203152654327425 1104 18-19 0.36058582113449944 1104 20-21 0.37303025288072433 1104 22-23 0.39172295383492894 1104 24-25 0.3704978973308499 1104 26-27 0.4122703126148366 1104 28-29 0.4096296346003001 1104 30-31 0.44333283279598845 1104 32-33 0.37993031823916823 1104 34-35 0.4140048523894606 1104 36-37 0.38838548972886144 1104 38-39 0.39845499043178734 1104 40-41 0.4479679587773191 1104 42-43 0.467204916323567 1104 44-45 0.3630400605560382 1104 46-47 0.40929030490246987 1104 48-49 0.4314970924496251 1104 50-51 0.4491894458651444 1104 52-53 0.47166832886908594 1104 54-55 0.436409864243231 1104 56-57 0.31603958576435787 1104 58-59 0.3597294004300622 1104 60-61 0.2208659754157054 1104 62-63 0.25076332572286475 1104 64-65 0.3222111691942331 1104 66-67 0.2454675247594622 1104 68-69 0.1970775968002343 1104 70-71 0.33895288173724936 1104 72-73 0.3285342085881737 1104 74-75 0.32675914966194597 1104 76-77 0.28113286070206556 1104 78-79 0.22691099970510464 1104 80-81 0.33212153510330467 1104 82-83 0.17455783963330873 1104 84-85 0.16815910638601395 1104 86-87 0.40829603868516173 1104 88-89 0.419930332897664 1104 90-91 0.44311366738216407 1104 92-93 0.32619101597340006 1104 94-95 0.4867802713732452 1104 96-97 0.4515402532245929 1104 98-99 0.6032895850532505 1104 100-101 0.737064586139283 1105 1 -0.12360111691741338 1105 2 0.04455641210625316 1105 3 0.03226737880365249 1105 4 0.049077823673016496 1105 5 0.018976752145370313 1105 6 0.0488527754262833 1105 7 0.05834017665253555 1105 8 -0.0029657607012154585 1105 9 -0.018059971710002287 1105 10-11 -0.01667605206407785 1105 12-13 0.05089679186009022 1105 14-15 0.03306985749603797 1105 16-17 0.04575462718891998 1105 18-19 0.010197756266428826 1105 20-21 -0.012100603222926054 1105 22-23 0.03371465502308979 1105 24-25 0.015035067748776498 1105 26-27 0.018404909264724267 1105 28-29 0.007723437913277564 1105 30-31 0.027155364167803953 1105 32-33 0.08923488499073073 1105 34-35 0.08053565959379227 1105 36-37 0.11085741252674808 1105 38-39 0.1193629382943513 1105 40-41 0.09585379292878571 1105 42-43 0.10519641439469041 1105 44-45 0.11559041341675425 1105 46-47 -0.12125708501208976 1105 48-49 0.15816371815679986 1105 50-51 0.1343289139631736 1105 52-53 0.12300758851483096 1105 54-55 0.1392450063054298 1105 56-57 0.01916799774199518 1105 58-59 0.14865842961464182 1105 60-61 0.15140880727470574 1105 62-63 0.19872848474438598 1105 64-65 0.20052809982902886 1105 66-67 0.219314037384855 1105 68-69 0.2206604616200707 1105 70-71 0.1449156428434648 1105 72-73 0.18470382559808485 1105 74-75 0.2542098134366597 1105 76-77 0.2412383954417976 1105 78-79 0.3612877496728686 1105 80-81 0.41059057246297215 1105 82-83 0.3262077454684942 1105 84-85 0.2708521872996883 1105 86-87 0.45747369299635565 1105 88-89 0.38501884634560923 1105 90-91 0.35618522549359355 1105 92-93 0.23376281270008192 1105 94-95 0.3395959156701558 1105 96-97 0.48561326760326295 1105 98-99 0.5993651307281205 1105 100-101 0.7580337526788696 1106 1 -0.18259047483483215 1106 2 -0.06248229339030331 1106 3 -0.048129323480964104 1106 4 -0.03327617775178027 1106 5 -0.39941974153738613 1106 6 -0.28605116266511743 1106 7 -0.14274990570499568 1106 8 -0.3576886291496564 1106 9 -0.48959681517700915 1106 10-11 -0.12416170745284916 1106 12-13 -0.07638169462192934 1106 14-15 -0.263861329607451 1106 16-17 -0.10692735485779536 1106 18-19 -0.19612155826973066 1106 20-21 -0.192018257890183 1106 22-23 -0.16391312696508464 1106 24-25 -0.11735934923071056 1106 26-27 -0.09461362692468356 1106 28-29 -0.1663700742865828 1106 30-31 -0.2461585175823089 1106 32-33 -0.1902430661167429 1106 34-35 -0.10516256546081593 1106 36-37 -0.08651221832273137 1106 38-39 -0.09224508037004142 1106 40-41 -0.07300490750095179 1106 42-43 -0.16227248480066692 1106 44-45 -0.1085681265843732 1106 46-47 -0.17148211353434561 1106 48-49 -0.06123808829034516 1106 50-51 -0.025234341422816442 1106 52-53 -0.1277265457623784 1106 54-55 -0.08538944037152163 1106 56-57 -0.1379827011227377 1106 58-59 -0.07539890146130546 1106 60-61 -0.19201847166938535 1106 62-63 -0.11612092324639178 1106 64-65 -0.14084278578916098 1106 66-67 -0.15225114475597934 1106 68-69 -0.15272627727350852 1106 70-71 -0.10324795863215996 1106 72-73 -0.06531228618235474 1106 74-75 -0.06848489342844744 1106 76-77 -0.1937682362616968 1106 78-79 -0.14256245391726452 1106 80-81 -0.10850748807247612 1106 82-83 -0.21251949416387106 1106 84-85 -0.1938079370913215 1106 86-87 0.018777011349083494 1106 88-89 0.020694285956466274 1106 90-91 -0.09881557011158648 1106 92-93 -0.2419028439978952 1106 94-95 -0.1418069199327121 1106 96-97 -0.05150607241347416 1106 98-99 0.039072379655610945 1106 100-101 0.05704492876520462 1107 1 -0.20639696014409736 1107 2 -0.1570114890353551 1107 3 -0.20959164823459986 1107 4 -0.1350045529151842 1107 5 -0.18248729612455605 1107 6 -0.168276276878629 1107 7 -0.15209170459305454 1107 8 -0.1327653219805427 1107 9 -0.24732213303465755 1107 10-11 -0.29424298051414866 1107 12-13 -0.2981949363324006 1107 14-15 -0.2768599832513061 1107 16-17 -0.26923551044681204 1107 18-19 -0.3034149433349853 1107 20-21 -0.24512727928207312 1107 22-23 -0.29837313690202905 1107 24-25 -0.3616286038565235 1107 26-27 -0.404051123566461 1107 28-29 -0.3893788195665664 1107 30-31 -0.5100221180567175 1107 32-33 -0.5199042765071127 1107 34-35 -0.41079231360932056 1107 36-37 -0.37468250675468084 1107 38-39 -0.31696198949757104 1107 40-41 -0.31628988057762086 1107 42-43 -0.34674847733749203 1107 44-45 -0.43350083792284977 1107 46-47 -0.42285963433446483 1107 48-49 -0.3679567756216855 1107 50-51 -0.2621077195591255 1107 52-53 -0.28418588534552924 1107 54-55 -0.38574847451913286 1107 56-57 -0.4790845434994253 1107 58-59 -0.3826512977113552 1107 60-61 -0.43788186206635515 1107 62-63 -0.37136123077824124 1107 64-65 -0.3144870299235123 1107 66-67 -0.3601489975989054 1107 68-69 -0.3462447629235115 1107 70-71 -0.31715034519719865 1107 72-73 -0.2674898814563207 1107 74-75 -0.2662522022885412 1107 76-77 -0.40420579110723764 1107 78-79 -0.37951307812166135 1107 80-81 -0.31001765872864695 1107 82-83 -0.3231235680297502 1107 84-85 -0.43091244396141803 1107 86-87 -0.21628885393993968 1107 88-89 -0.20631590523712973 1107 90-91 -0.40392294097774695 1107 92-93 -0.4704576206141766 1107 94-95 -0.4637616124388799 1107 96-97 -0.6063938672646358 1107 98-99 -0.6024685406585704 1107 100-101 -0.46507962117237867 1108 1 -0.3843091828776082 1108 2 -0.2840781542994222 1108 3 -0.3175914598475913 1108 4 -0.177666957749409 1108 5 -0.35894171729406565 1108 6 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9 -0.028976233924794315 2305 10-11 0.048259137987393785 2305 12-13 0.04189808936536821 2305 14-15 0.13509012829981515 2305 16-17 0.06572395694247035 2305 18-19 0.11977931599012237 2305 20-21 0.12440395482694555 2305 22-23 0.15995360150881766 2305 24-25 0.05705823795690179 2305 26-27 0.16335270931621437 2305 28-29 0.1186337474087722 2305 30-31 0.1330880750943777 2305 32-33 0.15151957261136317 2305 34-35 0.14185234807631986 2305 36-37 0.19034744587685282 2305 38-39 0.1788596483256626 2305 40-41 0.24718901438320273 2305 42-43 0.3066787579049475 2305 44-45 0.26808580078680677 2305 46-47 0.31449758237575764 2305 48-49 0.3133227323942478 2305 50-51 0.20438577229446508 2305 52-53 0.21486380612530098 2305 54-55 0.22392649637487239 2305 56-57 0.2916099370648766 2305 58-59 0.3264091485485707 2305 60-61 0.42069251536421604 2305 62-63 0.3759388788066431 2305 64-65 0.36793854276761095 2305 66-67 0.5157317775618822 2305 68-69 0.48386196663306436 2305 70-71 0.41733608156501845 2305 72-73 0.4656783930979387 2305 74-75 0.3393479540321991 2305 76-77 0.3467618318570871 2305 78-79 0.36482269168251236 2305 80-81 0.46156990149159327 2305 82-83 0.4855070522046532 2305 84-85 0.507674771237717 2305 86-87 0.6200218409892919 2305 88-89 0.568136043586783 2305 90-91 0.5538235641358717 2305 92-93 0.7592424253316459 2305 94-95 0.8500189622712497 2305 96-97 0.8094325921283811 2305 98-99 0.9346393965308444 2305 100-101 1.1148588590049116 2306 1 -0.5653832436899613 2306 2 -0.9638104627360065 2306 3 -0.7492029094216228 2306 4 -0.3672176290735365 2306 5 -0.20785961371256434 2306 6 -0.20560901810615917 2306 7 -0.0930483135703355 2306 8 -0.0850787873068839 2306 9 -0.10653852078485215 2306 10-11 -0.33449611690477354 2306 12-13 -0.25979549496714327 2306 14-15 -0.16634886449671882 2306 16-17 -0.2323283573537296 2306 18-19 -0.21121405829048 2306 20-21 -0.1780341896579074 2306 22-23 -0.10683846017611387 2306 24-25 -0.16678879667747992 2306 26-27 -0.16037302589813862 2306 28-29 -0.1468636913173782 2306 30-31 -0.0717534448605548 2306 32-33 -0.15269845786773573 2306 34-35 -0.2593052575149528 2306 36-37 -0.27022334762787636 2306 38-39 -0.15033763184855076 2306 40-41 -0.30403564573660447 2306 42-43 -0.1755233624821173 2306 44-45 -0.2839264232668057 2306 46-47 -0.20248093795431288 2306 48-49 -0.14154947186979427 2306 50-51 -0.10800967557880625 2306 52-53 -0.06344108022838668 2306 54-55 -0.1520296515320041 2306 56-57 -0.1446501260160602 2306 58-59 -0.20464683610077117 2306 60-61 -0.20669422718430042 2306 62-63 -0.02795479336337081 2306 64-65 -0.06616982806876592 2306 66-67 -0.01931391885756284 2306 68-69 -0.030878331047219376 2306 70-71 -0.008227084977249888 2306 72-73 -0.011617734339644414 2306 74-75 -0.038610026437027045 2306 76-77 -0.0325480881611071 2306 78-79 0.03840072393927585 2306 80-81 0.1949377968813053 2306 82-83 0.19951435761907987 2306 84-85 0.06549623103963853 2306 86-87 0.25104076918866625 2306 88-89 0.09989064361893796 2306 90-91 0.1845857440746741 2306 92-93 0.2212230631731451 2306 94-95 0.3829905431765219 2306 96-97 0.1565389866207596 2306 98-99 0.2689174950578668 2306 100-101 0.5920668221549832 2307 1 -1.6736747669972196 2307 2 -2.1979242366905467 2307 3 -1.7607254659287115 2307 4 -0.942494646729017 2307 5 -0.5547886971927554 2307 6 -0.4661382905095479 2307 7 -0.38921643002608874 2307 8 -0.5120661514054916 2307 9 -0.46038160620084767 2307 10-11 -0.37349920496264843 2307 12-13 -0.37084684824353786 2307 14-15 -0.7134107370790517 2307 16-17 -0.5436088203866021 2307 18-19 -0.4044485316409592 2307 20-21 -0.43178391822337403 2307 22-23 -0.5022316696323514 2307 24-25 -0.45433505339919833 2307 26-27 -0.44105565940947145 2307 28-29 -0.3876418305855154 2307 30-31 -0.7868790364928131 2307 32-33 -0.49889419293621984 2307 34-35 -0.48767807219964254 2307 36-37 -0.39169400371307006 2307 38-39 -0.37362458887850636 2307 40-41 -0.3596091352420032 2307 42-43 -0.34285369154392953 2307 44-45 -0.32594358695763503 2307 46-47 -0.4738985603635726 2307 48-49 -0.3552707847434178 2307 50-51 -0.30933411575843195 2307 52-53 -0.4047817185758973 2307 54-55 -0.35941368661963224 2307 56-57 -0.25524652649710333 2307 58-59 -0.3842478178266404 2307 60-61 -0.3894315013590841 2307 62-63 -0.2743024070439546 2307 64-65 -0.2632950274573318 2307 66-67 -0.3169110639126558 2307 68-69 -0.18947275629260218 2307 70-71 -0.27272930373187165 2307 72-73 -0.3197425956526345 2307 74-75 -0.3632688445077008 2307 76-77 -0.30126234730042967 2307 78-79 -0.33828397580067104 2307 80-81 -0.33368337743597465 2307 82-83 -0.26328908481232816 2307 84-85 -0.31346715867763564 2307 86-87 -0.26713852039171826 2307 88-89 -0.2650650965147463 2307 90-91 -0.2701904109297715 2307 92-93 -0.23534024771526774 2307 94-95 -0.14206497320482114 2307 96-97 -0.16369955860415786 2307 98-99 -0.1813998160747765 2307 100-101 0.04957682781412842 2308 1 -1.249095786245661 2308 2 -1.2173310657473415 2308 3 -0.7935373646984445 2308 4 -0.5065853581977819 2308 5 -0.6289852710235451 2308 6 -0.5802007106862064 2308 7 -1.4827511143025873 2308 8 -0.5973045346204557 2308 9 -0.5728066474055282 2308 10-11 -0.8238535958342936 2308 12-13 -0.8750952810745432 2308 14-15 -0.6847806398844298 2308 16-17 -0.8970242666443724 2308 18-19 -0.6216556119680945 2308 20-21 -0.6854380450506312 2308 22-23 -0.5494445310983451 2308 24-25 -0.558321119218057 2308 26-27 -0.5161923175443022 2308 28-29 -1.3355566589400851 2308 30-31 -0.6418988440825473 2308 32-33 -0.48035321031743194 2308 34-35 -1.1652646841374121 2308 36-37 -0.7089127795631711 2308 38-39 -0.4911656598514398 2308 40-41 -1.0245958149793708 2308 42-43 -0.4575355062938513 2308 44-45 -0.5204005056284728 2308 46-47 -0.48106658981691197 2308 48-49 -0.37609715638739516 2308 50-51 -0.472249736098739 2308 52-53 -0.5828934457322177 2308 54-55 -1.0939146311521597 2308 56-57 -1.7674207476504762 2308 58-59 -1.2236697070501634 2308 60-61 -0.8380932516104735 2308 62-63 -0.5046830967480247 2308 64-65 -0.37255315932087285 2308 66-67 -0.3397304624199293 2308 68-69 -0.34534259133523193 2308 70-71 -0.23532811998901337 2308 72-73 -0.2946597498218182 2308 74-75 -0.1340766574049468 2308 76-77 -1.1639220388956346 2308 78-79 -0.6395360768028198 2308 80-81 -0.12940299684243683 2308 82-83 -0.0551017626924164 2308 84-85 -0.03412303630354785 2308 86-87 -0.39543245609118216 2308 88-89 -0.3277974234630392 2308 90-91 -0.9516031621557772 2308 92-93 -0.06737160694152422 2308 94-95 0.17246619323619328 2308 96-97 0.09736916182651356 2308 98-99 -0.4381588909046066 2308 100-101 0.26643539756505064 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 16.0 12 152.0 13 757.0 14 2663.0 15 6027.0 16 10062.0 17 14931.0 18 21923.0 19 31447.0 20 31323.0 21 31696.0 22 33782.0 23 37739.0 24 44796.0 25 54554.0 26 68046.0 27 85846.0 28 107403.0 29 131947.0 30 164295.0 31 204175.0 32 257325.0 33 335358.0 34 466980.0 35 725430.0 36 1216684.0 37 2156067.0 38 1775937.0 39 227226.0 40 118.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 26.155430319862273 30.07065014539194 21.31428636606541 22.459633168680377 2 25.93313780401762 27.791644718034007 25.157930090001624 21.11728738794675 3 24.880589838892707 25.393465274385026 25.624377478261163 24.101567408461104 4 25.99632491176149 25.764151515519064 25.030031414120586 23.209492158598856 5 25.42741068358419 26.99484093124011 24.19272733226962 23.385021052906076 6 25.21859481708626 25.45541344955175 23.31366333741919 26.012328395942802 7 25.834238358529703 25.60016087899314 24.39558184367035 24.170018918806807 8 24.93467929602341 25.335967104224217 24.56977847360476 25.159575126147615 9 24.520013206050038 24.69254816363373 24.82817458863906 25.959264041677166 10-11 24.994178082274857 25.449433854888216 24.601265345301577 24.95512271753535 12-13 24.953018336857617 25.242385841290155 24.799938287672113 25.004657534180115 14-15 24.798955839055996 25.27250213652252 24.896327413009026 25.032214611412464 16-17 24.95155072843107 25.641951332163938 24.73521796896093 24.671279970444065 18-19 24.553295169045445 25.271719816328197 25.14876819137032 25.026216823256036 20-21 24.35289990934789 25.44125284758486 25.082374071313158 25.12347317175409 22-23 24.457154688111377 25.529406385139747 25.41666130969376 24.596777617055114 24-25 24.50342222654284 25.07145949603658 25.855544506714033 24.569573770706548 26-27 24.406170456532955 25.1158440337351 25.984176997942683 24.49380851178926 28-29 24.487574208225254 25.281999139811656 25.877102227781073 24.353324424182016 30-31 24.53390697012119 25.101452980858262 25.859424217188582 24.50521583183197 32-33 24.421768344438238 25.159817706052827 25.948184204506504 24.470229745002428 34-35 24.50612550647652 25.31274614279757 25.81862227713153 24.362506073594375 36-37 24.498156756590227 25.208006204223654 25.748122492178616 24.545714547007496 38-39 24.424958270191805 25.265006417451392 25.82063569034481 24.48939962201199 40-41 24.609325062652328 25.44169555591188 25.43879066154694 24.51018871988886 42-43 24.66632527588006 25.36856377898739 25.372220671058493 24.59289027407405 44-45 24.513554516073707 25.467196768447536 25.499841959191755 24.519406756287005 46-47 24.600131284244704 25.642072622116547 25.33083653922893 24.426959554409823 48-49 24.607608809822935 25.53072844562317 25.29735444781172 24.564308296742176 50-51 24.498593400419615 25.58530285979876 25.456505059125213 24.45959868065641 52-53 24.541414818187572 25.712020537785097 25.36553759466984 24.38102704935749 54-55 24.578669027130864 25.572676575732356 25.333013693878232 24.51564070325855 56-57 24.494821282893533 25.640356369287154 25.456074479793457 24.408747868025856 58-59 24.528545954215954 25.77008810259577 25.335324267475396 24.366041675712875 60-61 24.565381712822752 25.652109365694788 25.268796728470367 24.513712193012097 62-63 24.520607526817813 25.71974064326854 25.325105588968245 24.4345462409454 64-65 24.555526904173416 25.880486217458813 25.20979523102461 24.354191647343157 66-67 24.566255000481522 25.777511047695334 25.136020617351303 24.52021333447184 68-69 24.51810895379411 25.875561845382965 25.17873287416192 24.42759632666101 70-71 24.596268199254165 26.004371775051773 25.08034852910462 24.31901149658945 72-73 24.61943458020213 25.885101300155515 25.013790667611435 24.48167345203092 74-75 24.504833768481255 25.980805137260198 25.12287278648869 24.39148830776986 76-77 24.560366373282445 26.132896673331953 24.95748787161119 24.349249081774413 78-79 24.58584332782758 26.005790867497275 24.969240868018826 24.439124936656324 80-81 24.456899979210903 26.10860836032237 25.059116722900733 24.375374937565997 82-83 24.550220468746854 26.243779948005425 24.910973174786342 24.295026408461382 84-85 24.560869726585764 26.13651717841728 24.874167738667698 24.428445356329263 86-87 24.469847681651714 26.226762967654636 24.95888270606617 24.344506644627472 88-89 24.587292742761232 26.350891105152808 24.816700559122424 24.245115592963533 90-91 24.556381998339297 26.239413509711568 24.81022974015083 24.393974751798307 92-93 24.453165731632257 26.316591902524593 24.85106350978819 24.379178856054956 94-95 24.58330528502839 26.464882612537384 24.721430299810606 24.230381802623626 96-97 24.536041673287077 26.389588664532127 24.664481668600434 24.409887993580366 98-99 24.449781741749295 26.48882971016976 24.742405899155525 24.31898264892542 100-101 24.61433573687641 26.655173227272517 24.33827972103545 24.392211314815626 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 44715.0 1 25690.5 2 4801.5 3 2661.5 4 2243.5 5 1994.5 6 1839.5 7 1783.5 8 1780.0 9 1722.5 10 1686.0 11 1766.0 12 2181.0 13 2924.5 14 3800.0 15 4001.0 16 9026.0 17 10146.0 18 5712.0 19 5136.0 20 4941.5 21 5518.5 22 6596.0 23 8212.5 24 9938.0 25 12173.5 26 15527.5 27 19164.0 28 23359.0 29 28589.0 30 35083.5 31 41884.5 32 49254.5 33 58719.0 34 68842.0 35 80045.5 36 96365.5 37 113786.5 38 130993.0 39 150025.0 40 168081.5 41 183741.5 42 200446.0 43 219195.5 44 239808.0 45 263316.5 46 287668.0 47 314839.0 48 341024.5 49 359747.0 50 368017.5 51 373680.0 52 383534.5 53 392885.0 54 393408.0 55 382518.5 56 359926.0 57 328071.5 58 296627.5 59 265788.0 60 232243.0 61 196987.5 62 160654.5 63 123513.0 64 89942.5 65 63602.0 66 44846.0 67 33157.0 68 24829.0 69 18636.5 70 14512.0 71 11172.0 72 9001.5 73 7147.5 74 5453.0 75 4627.5 76 3753.0 77 2421.5 78 1403.0 79 796.5 80 456.5 81 314.0 82 242.5 83 180.0 84 115.0 85 66.0 86 30.0 87 5.0 88 2.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04286386925137173 2 9.339326350751622E-4 3 1.698059336500295E-4 4 7.277397156429835E-5 5 1.2128995260716392E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 6.064497630358196E-6 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 6.064497630358196E-6 94-95 1.2128995260716392E-5 96-97 0.0 98-99 6.064497630358196E-6 100-101 3.0322488151790978E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8244706.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.774000105241825 #Duplication Level Percentage of deduplicated Percentage of total 1 80.89017332517844 43.497881889011744 2 10.084507307236883 10.845685940013361 3 3.080932981532842 4.970222714195701 4 1.3751175021015163 2.957822748109072 5 0.7643933461162239 2.0552243937249983 6 0.5279528155778206 1.7034080856266665 7 0.3833293125245845 1.4429205344427498 8 0.3003737481242605 1.2921838370596692 9 0.24740917826482667 1.1973763060245526 >10 2.2038658094250385 23.570057321729706 >50 0.13288364669166974 4.6225617976314295 >100 0.00874514113381764 0.607723569507044 >500 6.769229277691057E-5 0.031158875643391336 >1k 1.3537844126536002E-4 0.16411185966022818 >5k 6.768921478655647E-5 0.2254930342773207 >10k+ 4.512614319103765E-5 0.8161670933423089 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 48654 0.5901241354148953 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18614 0.22576911778297493 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.6386985782149175E-5 0.0 0.0 0.0 0.0 2 3.6386985782149175E-5 0.0 0.0 1.2128995260716392E-5 1.2128995260716392E-5 3 3.6386985782149175E-5 0.0 0.0 3.6386985782149175E-5 1.2128995260716392E-5 4 3.6386985782149175E-5 0.0 0.0 3.6386985782149175E-5 1.2128995260716392E-5 5 3.6386985782149175E-5 0.0 0.0 4.851598104286557E-5 4.851598104286557E-5 6 4.851598104286557E-5 1.2128995260716392E-5 0.0 4.851598104286557E-5 4.851598104286557E-5 7 6.064497630358196E-5 1.2128995260716392E-5 0.0 4.851598104286557E-5 7.277397156429835E-5 8 6.064497630358196E-5 1.2128995260716392E-5 0.0 6.064497630358196E-5 8.490296682501474E-5 9 7.277397156429835E-5 1.2128995260716392E-5 0.0 6.064497630358196E-5 1.0916095734644752E-4 10-11 8.490296682501475E-5 1.2128995260716392E-5 0.0 6.064497630358196E-5 1.2128995260716391E-4 12-13 1.0916095734644752E-4 1.2128995260716392E-5 0.0 6.064497630358196E-5 1.7587043128038769E-4 14-15 1.3341894786788032E-4 1.2128995260716392E-5 0.0 6.064497630358196E-5 2.0619291943217867E-4 16-17 1.7587043128038766E-4 1.2128995260716392E-5 0.0 6.064497630358196E-5 2.7896689099647697E-4 18-19 2.1225741706253686E-4 1.2128995260716392E-5 0.0 6.064497630358196E-5 3.335473696697008E-4 20-21 2.48644402844686E-4 1.2128995260716392E-5 0.0 6.064497630358196E-5 3.759988530822082E-4 22-23 2.6683789573576065E-4 1.2128995260716392E-5 0.0 6.670947393394016E-5 4.245148341250737E-4 24-25 2.850313886268352E-4 1.2128995260716392E-5 0.0 8.490296682501474E-5 4.912243080590139E-4 26-27 3.3961186730005894E-4 1.2128995260716392E-5 0.0 8.490296682501474E-5 5.518692843625957E-4 28-29 3.3961186730005894E-4 1.2128995260716392E-5 0.0 8.490296682501474E-5 5.821917725143868E-4 30-31 3.6993435545184996E-4 1.2128995260716392E-5 0.0 8.490296682501474E-5 6.489012464483269E-4 32-33 4.1238583886435735E-4 1.2128995260716392E-5 0.0 9.096746445537294E-5 7.701911990554909E-4 34-35 4.487728246465065E-4 1.2128995260716392E-5 0.0 9.703196208573114E-5 8.308361753590729E-4 36-37 4.6696631753758106E-4 1.2128995260716392E-5 0.0 1.0309645971608934E-4 8.97545649293013E-4 38-39 4.912243080590138E-4 1.8193492891074587E-5 0.0 1.0916095734644752E-4 9.339326350751621E-4 40-41 5.276112938411631E-4 2.4257990521432784E-5 0.0 1.0916095734644752E-4 9.763841184876696E-4 42-43 5.579337819929539E-4 3.032248815179098E-5 0.0 1.0916095734644752E-4 0.0010188356019001769 44-45 5.761272748840286E-4 3.6386985782149175E-5 0.0 1.2128995260716392E-4 0.0010916095734644752 46-47 6.064497630358196E-4 3.6386985782149175E-5 0.0 1.2128995260716392E-4 0.0011583190473984154 48-49 6.367722511876105E-4 3.6386985782149175E-5 0.0 1.3948344549823848E-4 0.0011825770379198482 50-51 7.216752180126253E-4 3.6386985782149175E-5 0.0 1.455479431285967E-4 0.001212899526071639 52-53 0.0010067066066394605 4.2451483412507375E-5 0.0 1.6374143601967129E-4 0.0012432220142234301 54-55 0.001394834454982385 4.851598104286557E-5 0.0 1.6980593365002947E-4 0.0012917379952662959 56-57 0.0020376712038003538 4.851598104286557E-5 0.0 1.6980593365002947E-4 0.0013402539763091611 58-59 0.0028563783838987103 4.851598104286557E-5 0.0 1.6980593365002947E-4 0.0013887699573520268 60-61 0.003984374943145335 4.851598104286557E-5 0.0 1.8193492891074587E-4 0.0014433504360252506 62-63 0.005421660881540227 4.851598104286557E-5 0.0 1.9406392417146227E-4 0.0014615439289163252 64-65 0.007647331511881685 4.851598104286557E-5 0.0 1.9406392417146227E-4 0.0015100599099591907 66-67 0.010540096881562544 4.851598104286557E-5 0.0 2.0619291943217867E-4 0.0015585758910020564 68-69 0.014178795459777462 4.851598104286557E-5 0.0 2.1832191469289505E-4 0.0015888983791538473 70-71 0.018969748587760436 4.851598104286557E-5 0.0 2.1832191469289505E-4 0.0016313498625663548 72-73 0.025325342104375828 4.851598104286557E-5 0.0 2.1832191469289505E-4 0.0017101883317610113 74-75 0.03424621811863274 4.851598104286557E-5 0.0 2.3651540758396963E-4 0.0017708333080645932 76-77 0.045823344094986534 4.851598104286557E-5 0.0 2.4257990521432785E-4 0.0018193492891074587 78-79 0.06074200826566769 6.064497630358196E-5 0.0 2.4257990521432785E-4 0.0019042522559324735 80-81 0.07994220776338173 6.064497630358196E-5 0.0 2.4257990521432785E-4 0.001970961729866414 82-83 0.10405465034168593 6.064497630358196E-5 0.0 2.850313886268352E-4 0.0020012842180182045 84-85 0.1353535226119646 6.064497630358196E-5 0.0 2.910958862571934E-4 0.0020679936919521446 86-87 0.1722984421761067 6.064497630358196E-5 0.0 2.910958862571934E-4 0.0021225741706253688 88-89 0.2192194603421881 6.064497630358196E-5 0.0 3.0322488151790977E-4 0.0021286386682557268 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 5515 0.0 90.384285 1 TCGACGG 5770 0.0 86.52147 2 ACGGCGC 7245 0.0 69.036964 5 CGACGGC 7765 0.0 64.65884 3 CGGCGCG 7895 0.0 63.29295 6 GACGGCG 8040 0.0 62.387787 4 ATCTCGA 4105 0.0 56.258224 1 CGCGCCG 13315 0.0 43.914566 9 TCTCGAG 5435 0.0 42.73722 2 GCGCGCC 15325 0.0 38.154808 8 GGCGCGC 15460 0.0 37.821636 7 TCGAGGG 6440 0.0 35.84612 4 CTCGAGG 6900 0.0 33.869625 3 GGGCGCG 7180 0.0 32.283985 8 AGGGCGC 7580 0.0 30.643007 7 CGAGGGC 7730 0.0 30.109833 5 TCCATAT 10175 0.0 25.068686 18-19 CCATATT 10305 0.0 25.05205 18-19 GAGGGCG 9990 0.0 23.535934 6 ATCCATA 10985 0.0 23.28506 16-17 >>END_MODULE