FastQCFastQC Report
Tue 31 May 2016
SRR1051269_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051269_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7823080
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT3499444.473225379262388No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT662110.8463546327022095No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT152520.1949615752363519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG570300.094.311741
TCGACGG579300.092.838322
TATCTCG467350.091.8360062
ATATCTC473300.091.012641
ACGGCGC593800.090.3713465
GACGGCG594850.090.2677154
CGACGGC598600.089.74193
CGGCGCG598550.089.733526
ATCTCGA487350.087.969763
TCTCGAG495550.085.5748754
CTCGAGG513000.082.6639945
TCGAGGG515150.082.3189856
CGAGGGC521600.081.246947
AGGGCGC524700.080.947959
GAGGGCG537150.079.0982748
CGCGCCG1104750.049.503249
GGCGCGC1125850.048.5881357
GCGCGCC1128800.048.5621388
TCCATAT633550.042.37688418-19
CCATATT636950.042.1693218-19
Produced by FastQC (version 0.11.5)