##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1051269_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7823080
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0506029594482	33.0	31.0	34.0	30.0	34.0
2	32.282505483773654	34.0	31.0	34.0	30.0	34.0
3	32.373573579715405	34.0	31.0	34.0	30.0	34.0
4	35.79059922690296	37.0	35.0	37.0	35.0	37.0
5	35.776557315021705	37.0	35.0	37.0	35.0	37.0
6	35.781259427233266	37.0	35.0	37.0	35.0	37.0
7	35.727927363647055	37.0	35.0	37.0	33.0	37.0
8	35.71204960194706	37.0	35.0	37.0	33.0	37.0
9	37.41172223727739	39.0	37.0	39.0	34.0	39.0
10-11	37.395057956712705	39.0	37.0	39.0	34.0	39.0
12-13	37.349008510714455	39.0	37.0	39.0	34.0	39.0
14-15	38.693218591654436	40.0	38.0	41.0	34.0	41.0
16-17	38.551179906123934	40.0	38.0	41.0	33.5	41.0
18-19	38.58232614264459	40.0	38.0	41.0	34.0	41.0
20-21	38.578712987723506	40.0	38.0	41.0	34.0	41.0
22-23	38.53417209078777	40.0	38.0	41.0	34.0	41.0
24-25	38.520118866226596	40.0	38.0	41.0	34.0	41.0
26-27	38.432359888944	40.0	38.0	41.0	34.0	41.0
28-29	38.2001240559984	40.0	38.0	41.0	33.5	41.0
30-31	37.64313882767401	39.5	36.5	41.0	33.0	41.0
32-33	37.22960617812933	39.0	35.5	41.0	33.0	41.0
34-35	36.99406512524479	39.0	35.0	40.0	32.0	41.0
36-37	37.13609121982646	39.0	35.0	41.0	32.0	41.0
38-39	36.95788218962352	39.0	35.0	41.0	31.5	41.0
40-41	36.1716800799685	39.0	35.0	41.0	27.5	41.0
42-43	36.17611330831335	39.0	35.0	41.0	28.0	41.0
44-45	36.106148856971934	39.0	35.0	41.0	28.0	41.0
46-47	36.110535300674414	39.0	35.0	41.0	28.0	41.0
48-49	35.97109450497758	39.0	35.0	41.0	27.0	41.0
50-51	35.700452890677326	38.5	34.5	40.5	27.0	41.0
52-53	35.4464527398416	38.0	34.0	40.0	27.0	41.0
54-55	35.140148573196235	38.0	34.0	40.0	26.0	41.0
56-57	34.72558020626147	38.0	34.0	40.0	25.0	41.0
58-59	34.39005826349724	37.0	34.0	40.0	25.0	41.0
60-61	34.21267154368867	37.0	34.0	40.0	25.0	41.0
62-63	33.88285330074599	36.5	34.0	39.5	24.0	41.0
64-65	33.506782047480016	36.0	34.0	39.0	23.0	41.0
66-67	33.02345891899354	35.0	33.0	39.0	21.5	41.0
68-69	32.563115358656695	35.0	33.0	38.5	20.0	40.0
70-71	32.08374770550729	35.0	32.5	37.0	18.5	39.5
72-73	31.69643203188514	35.0	32.0	37.0	18.0	39.0
74-75	31.319874269469313	35.0	32.0	36.0	15.5	39.0
76-77	30.88836353456695	35.0	31.5	36.0	9.5	37.5
78-79	30.598683894322953	35.0	31.0	35.0	7.5	37.0
80-81	30.33678774855939	35.0	31.0	35.0	7.0	37.0
82-83	30.062038992315046	35.0	31.0	35.0	2.0	36.0
84-85	29.760900885584704	35.0	31.0	35.0	2.0	36.0
86-87	29.498173034150234	35.0	30.5	35.0	2.0	35.5
88-89	29.22789106080981	34.0	30.0	35.0	2.0	35.0
90-91	29.022628108110872	34.0	30.0	35.0	2.0	35.0
92-93	28.82951490972865	34.0	30.0	35.0	2.0	35.0
94-95	28.535688565117574	34.0	29.5	35.0	2.0	35.0
96-97	28.21400202989104	34.0	29.0	35.0	2.0	35.0
98-99	27.86493427141228	34.0	29.0	35.0	2.0	35.0
100-101	26.653809816593977	33.0	26.5	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3463243434594574
1101	2	0.33977740139648915
1101	3	0.3637663165759335
1101	4	0.33679282474636096
1101	5	0.3580483556759688
1101	6	0.34496183936129654
1101	7	0.3422301411311608
1101	8	0.28718001805962245
1101	9	0.4095551809760707
1101	10-11	0.35715026533762284
1101	12-13	0.38441408416589695
1101	14-15	0.5010629727193674
1101	16-17	0.3623572963603152
1101	18-19	0.45946604573387617
1101	20-21	0.4520354693979627
1101	22-23	0.3971280336036429
1101	24-25	0.46816180811470787
1101	26-27	0.5441218862836124
1101	28-29	0.6333059590775676
1101	30-31	0.6548017206209025
1101	32-33	0.6136942552074132
1101	34-35	0.6573987469244145
1101	36-37	0.6704340602020338
1101	38-39	0.6750847566656333
1101	40-41	0.593789128115084
1101	42-43	0.5526970885372648
1101	44-45	0.5693104928360029
1101	46-47	0.5345630882059851
1101	48-49	0.5660868987677574
1101	50-51	0.5230377014474783
1101	52-53	0.4859165636387175
1101	54-55	0.537087958250936
1101	56-57	0.5005207711578095
1101	58-59	0.4431784908635876
1101	60-61	0.41302894420191194
1101	62-63	0.489886370005749
1101	64-65	0.437508883943984
1101	66-67	0.37920609261906435
1101	68-69	0.3808060859276239
1101	70-71	0.5056109102247177
1101	72-73	0.5063071135306529
1101	74-75	0.44282140900012124
1101	76-77	0.40262336673391275
1101	78-79	0.27389991752029275
1101	80-81	0.2787390996878294
1101	82-83	0.33895739398440483
1101	84-85	0.3343651871537823
1101	86-87	0.32027696356353985
1101	88-89	0.34406175896569735
1101	90-91	0.41716035930938133
1101	92-93	0.3712700370039528
1101	94-95	0.43625949509057804
1101	96-97	0.42383460366104586
1101	98-99	0.44223983386679677
1101	100-101	0.5899571869143294
1102	1	0.35290791696925794
1102	2	0.3791776426364706
1102	3	0.37512764413144595
1102	4	0.3222742234774145
1102	5	0.318458214757662
1102	6	0.2913797018143285
1102	7	0.3476597671353332
1102	8	0.3088091021305459
1102	9	0.3431201402184456
1102	10-11	0.36140980651784105
1102	12-13	0.35515262552513605
1102	14-15	0.5056555261113616
1102	16-17	0.463614140503509
1102	18-19	0.5553101766687547
1102	20-21	0.4380125967618014
1102	22-23	0.46812686584524243
1102	24-25	0.5329606004967715
1102	26-27	0.5260905379364473
1102	28-29	0.5845479888302023
1102	30-31	0.601734010870473
1102	32-33	0.5806773527143392
1102	34-35	0.6821678872610306
1102	36-37	0.695377378333049
1102	38-39	0.7027545568893245
1102	40-41	0.6239314046098983
1102	42-43	0.6031865478034248
1102	44-45	0.63504928862573
1102	46-47	0.610853617616371
1102	48-49	0.7233958087314178
1102	50-51	0.639269907746062
1102	52-53	0.5909708982110331
1102	54-55	0.5660173832587105
1102	56-57	0.6338487398483181
1102	58-59	0.5847613533343434
1102	60-61	0.5233740896829744
1102	62-63	0.5995652833460525
1102	64-65	0.553365389838639
1102	66-67	0.5186084507280881
1102	68-69	0.507442685114448
1102	70-71	0.6135033771915914
1102	72-73	0.6356372877900611
1102	74-75	0.6222082106604425
1102	76-77	0.5072857574174279
1102	78-79	0.4107160903480924
1102	80-81	0.4721705427466567
1102	82-83	0.500360262209945
1102	84-85	0.46857105642569863
1102	86-87	0.46826398266037117
1102	88-89	0.5617616885227932
1102	90-91	0.6116449654893898
1102	92-93	0.5294837769297587
1102	94-95	0.5801027822689626
1102	96-97	0.5694901356888629
1102	98-99	0.6497215913116001
1102	100-101	0.7304024746105888
1103	1	0.23318503103179467
1103	2	0.23304891182126397
1103	3	0.2823387945240299
1103	4	0.2156503645213661
1103	5	0.26649857576104097
1103	6	0.2554396820321898
1103	7	0.3142482252739569
1103	8	0.2707953001875367
1103	9	0.3472991108364667
1103	10-11	0.3288523242751751
1103	12-13	0.3359027134505794
1103	14-15	0.4195191419604072
1103	16-17	0.5051029169573269
1103	18-19	0.46379976672761813
1103	20-21	0.43366895427073615
1103	22-23	0.46229048289036
1103	24-25	0.4660877449599141
1103	26-27	0.44855967649086637
1103	28-29	0.460618756306026
1103	30-31	0.5193975027905182
1103	32-33	0.4981838782938155
1103	34-35	0.5137077542319872
1103	36-37	0.4315219819988272
1103	38-39	0.4213156061274148
1103	40-41	0.4933281218925174
1103	42-43	0.46385355449693577
1103	44-45	0.40838847236411624
1103	46-47	0.41847408076716874
1103	48-49	0.42068513562796284
1103	50-51	0.36043933961671115
1103	52-53	0.384552923434093
1103	54-55	0.27956032792061336
1103	56-57	0.3717905460195823
1103	58-59	0.3421524025894982
1103	60-61	0.2876733452810498
1103	62-63	0.41858947438092997
1103	64-65	0.4323540535567574
1103	66-67	0.38357393838987264
1103	68-69	0.3908973657700727
1103	70-71	0.48698243809315045
1103	72-73	0.47407094606361255
1103	74-75	0.4911482611470426
1103	76-77	0.5205176906502551
1103	78-79	0.3853107102062019
1103	80-81	0.3721532060340351
1103	82-83	0.5006616227064917
1103	84-85	0.4485922497289607
1103	86-87	0.49024317458750843
1103	88-89	0.5795548851264023
1103	90-91	0.5667940027592806
1103	92-93	0.5003418649428539
1103	94-95	0.5166689476948427
1103	96-97	0.49907196612816307
1103	98-99	0.5901045547209556
1103	100-101	0.6926125827915577
1104	1	0.28766423960242804
1104	2	0.280894427343128
1104	3	0.2536038425043543
1104	4	0.23434838607540343
1104	5	0.2154689533391405
1104	6	0.27013874689575346
1104	7	0.2723482494568614
1104	8	0.23960898030760092
1104	9	0.28834572875633313
1104	10-11	0.2759646869748167
1104	12-13	0.3417165798492192
1104	14-15	0.3988070619650941
1104	16-17	0.4621528067493017
1104	18-19	0.4185949627694967
1104	20-21	0.46895487471211084
1104	22-23	0.5125483946783689
1104	24-25	0.4361888471353055
1104	26-27	0.46980104965790304
1104	28-29	0.4756287293975916
1104	30-31	0.560648273492184
1104	32-33	0.49702114749688775
1104	34-35	0.5440699333337164
1104	36-37	0.5301033492750378
1104	38-39	0.4974664770018151
1104	40-41	0.4051221625212662
1104	42-43	0.45181567153299795
1104	44-45	0.4282910978842551
1104	46-47	0.4667363206685735
1104	48-49	0.42559736412953697
1104	50-51	0.35458591748550106
1104	52-53	0.3117956718712449
1104	54-55	0.3263959168950663
1104	56-57	0.3825667897378793
1104	58-59	0.4069929360177724
1104	60-61	0.31705427372354933
1104	62-63	0.4048459920704488
1104	64-65	0.3798642570325299
1104	66-67	0.49079210084268965
1104	68-69	0.5292404923228062
1104	70-71	0.5403097149939313
1104	72-73	0.5151828383767239
1104	74-75	0.4974214621709301
1104	76-77	0.542414605241941
1104	78-79	0.4330082963795441
1104	80-81	0.45866976262522385
1104	82-83	0.44579791508221334
1104	84-85	0.4562019085769755
1104	86-87	0.5016685172142381
1104	88-89	0.5474899509014115
1104	90-91	0.6366042226724993
1104	92-93	0.5610963158357904
1104	94-95	0.627636299852032
1104	96-97	0.6965517136865458
1104	98-99	0.7219630582365539
1104	100-101	0.7657347297212347
1105	1	0.10549988011519673
1105	2	0.08375383033789063
1105	3	0.09987616263730814
1105	4	-0.03667616316981537
1105	5	0.105586750563333
1105	6	0.08083816151594192
1105	7	0.09405773991789346
1105	8	0.09821623662328705
1105	9	0.1561995837826089
1105	10-11	0.06679725330202757
1105	12-13	0.1377478622570436
1105	14-15	0.21795102522737153
1105	16-17	0.08211666979376275
1105	18-19	0.1415979975335091
1105	20-21	0.11686956190541054
1105	22-23	0.07804016152833526
1105	24-25	0.11105776007951818
1105	26-27	0.1414194050142541
1105	28-29	0.2305129532802752
1105	30-31	0.25572230840207055
1105	32-33	0.18796424122459854
1105	34-35	0.26924246744087554
1105	36-37	0.2113874351869498
1105	38-39	0.24815222540963333
1105	40-41	0.18185879964864426
1105	42-43	0.14712686107645823
1105	44-45	0.050289715953006464
1105	46-47	0.10690370171754182
1105	48-49	0.11525030561364957
1105	50-51	0.1163585349940135
1105	52-53	0.022893261454619562
1105	54-55	0.11864152089496116
1105	56-57	0.143539059577094
1105	58-59	0.14567776012704314
1105	60-61	0.052948958166133764
1105	62-63	0.17246785061696812
1105	64-65	0.0960105836418279
1105	66-67	0.05917156525725176
1105	68-69	0.14069792393465264
1105	70-71	0.17779606047832175
1105	72-73	0.23478872971566034
1105	74-75	0.2059318160703114
1105	76-77	0.3216855820776807
1105	78-79	0.2691224698790826
1105	80-81	0.2482733009473428
1105	82-83	0.2251987201247978
1105	84-85	0.1372323891200331
1105	86-87	0.21036699780788481
1105	88-89	0.24930909416981706
1105	90-91	0.3192428045697824
1105	92-93	0.2720042905732001
1105	94-95	0.3861562057165351
1105	96-97	0.321268594274148
1105	98-99	0.30847042225967414
1105	100-101	0.36595367447367977
1106	1	-0.10838578190725556
1106	2	-0.1445888957892265
1106	3	-0.16913678904382579
1106	4	-0.3215357942700052
1106	5	-0.11961094662841987
1106	6	-0.09528584702419352
1106	7	-0.1096956765947894
1106	8	-0.08706965969829383
1106	9	-0.13382087028956846
1106	10-11	-0.10464672046624912
1106	12-13	-0.09408771375517944
1106	14-15	-0.27377749622993264
1106	16-17	-0.22366777205137112
1106	18-19	-0.2485339306737373
1106	20-21	-0.35905133945425405
1106	22-23	-0.19760359001586636
1106	24-25	-0.1015434979759462
1106	26-27	-0.44171842032756814
1106	28-29	-0.2520426545200465
1106	30-31	-0.2054536523273427
1106	32-33	-0.2746797858680594
1106	34-35	-0.15384149437704764
1106	36-37	-0.356971503769671
1106	38-39	-0.2354479134573424
1106	40-41	-0.20260048421827292
1106	42-43	-0.2792987914076761
1106	44-45	-0.29429231952037327
1106	46-47	-0.10874767205814351
1106	48-49	-0.07918201031390737
1106	50-51	-0.2801079902639074
1106	52-53	-0.30112823509448816
1106	54-55	-0.2809293958551038
1106	56-57	-0.1530831168991682
1106	58-59	-0.11188623576178713
1106	60-61	-0.13237539720967106
1106	62-63	-0.007001248489842737
1106	64-65	-0.0772512412167643
1106	66-67	-0.11376972983160982
1106	68-69	-0.029769123052268753
1106	70-71	0.07651042499542626
1106	72-73	-0.022492219216658782
1106	74-75	0.06611539604278605
1106	76-77	0.1298610252928718
1106	78-79	-0.06437706162528656
1106	80-81	-0.06683930269048233
1106	82-83	0.05354689093350018
1106	84-85	-0.024019927209387504
1106	86-87	-0.0020904284838074716
1106	88-89	0.12728183270627014
1106	90-91	0.115629923192202
1106	92-93	0.044521747327358696
1106	94-95	0.06455530274170229
1106	96-97	-0.13602278736645346
1106	98-99	0.014576994264334786
1106	100-101	0.06302379840310124
1107	1	-0.24284057806108095
1107	2	-0.3055832353878358
1107	3	-0.3221907557651562
1107	4	-0.22525112034766437
1107	5	-0.3282262009008221
1107	6	-0.18808131480206924
1107	7	-0.21688433287059183
1107	8	-0.21791989198449357
1107	9	-0.19734140630785646
1107	10-11	-0.23533299784734396
1107	12-13	-0.15048278254203495
1107	14-15	-0.21030635828365263
1107	16-17	-0.26798681792945445
1107	18-19	-0.32844712305613655
1107	20-21	-0.3056087312218665
1107	22-23	-0.25779751535038287
1107	24-25	-0.38001950642731686
1107	26-27	-0.27735028845862786
1107	28-29	-0.3118039943033253
1107	30-31	-0.208530154794893
1107	32-33	-0.2577118746960494
1107	34-35	-0.4170098973546672
1107	36-37	-0.2806128403127417
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2308	30-31	-1.4762386852995562
2308	32-33	-0.7526833021217101
2308	34-35	-1.0448525613315383
2308	36-37	-1.2511084381937039
2308	38-39	-0.8275241615686966
2308	40-41	-0.9464569592701011
2308	42-43	-0.7277487021238116
2308	44-45	-0.937143071008677
2308	46-47	-1.1793165724064067
2308	48-49	-1.8951070879736491
2308	50-51	-2.7626628219530147
2308	52-53	-1.9785787699888644
2308	54-55	-1.4775797167436835
2308	56-57	-0.7090272696420996
2308	58-59	-0.829188468380174
2308	60-61	-0.3416799361712677
2308	62-63	-0.2910376479905068
2308	64-65	-0.27118309928621187
2308	66-67	-0.35233460204690203
2308	68-69	-0.7755140156021838
2308	70-71	-0.9959139088183875
2308	72-73	-1.1053483956520473
2308	74-75	-1.0844618670567279
2308	76-77	-0.7904433000108213
2308	78-79	-0.5195309709797264
2308	80-81	-0.6746503535860633
2308	82-83	-0.624658525446371
2308	84-85	-0.9937229759164872
2308	86-87	-0.7862039924934692
2308	88-89	-0.8423344782057249
2308	90-91	-1.240756727860969
2308	92-93	-0.9321862564170615
2308	94-95	-1.8230033282198939
2308	96-97	-1.1521056101622271
2308	98-99	-1.6875385308895368
2308	100-101	-1.9134238549672027
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	157.0
11	1177.0
12	3313.0
13	6372.0
14	9744.0
15	13444.0
16	18988.0
17	29597.0
18	59330.0
19	145344.0
20	154076.0
21	105412.0
22	92779.0
23	89361.0
24	93475.0
25	101135.0
26	114274.0
27	127308.0
28	139537.0
29	151719.0
30	167842.0
31	194447.0
32	231073.0
33	294289.0
34	413102.0
35	638573.0
36	1074923.0
37	1777653.0
38	1395607.0
39	178950.0
40	78.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	28.022990944742993	30.34791923385674	20.378559339799672	21.250530481600595
2	22.499424778987304	23.797724170019993	34.445320768802056	19.257530282190647
3	21.827937334144607	27.808701943480063	21.678431001600394	28.68492972077494
4	29.516060170674468	21.706016044831447	27.3504425366991	21.427481247794987
5	22.444868261605404	29.375936331981777	21.361420310159172	26.817775096253648
6	22.4148800728102	21.604329752476005	26.466046621023942	29.514743553689847
7	29.67894997878074	21.773483078276076	21.144127376941054	27.403439566002135
8	34.905446448201985	21.714030790941674	21.407783123782448	21.97273963707389
9	21.83151648711249	27.0807022298123	21.621880384707815	29.465900898367398
10-11	31.357579112063277	21.642601123854032	21.471517611989142	25.528302152093552
12-13	28.399922306810254	21.578957493657068	21.59041078388632	28.430709415646362
14-15	28.208966289492114	21.64821272440011	21.70648261298619	28.436338373121583
16-17	28.29262259877184	25.242749658702202	21.676718121251476	24.787909621274483
18-19	24.560256318483255	21.661449199036696	25.368492972077494	28.409801510402556
20-21	24.376933381737118	28.03349192389698	22.062563593878625	25.52701110048727
22-23	21.45324475781917	25.07675365712737	28.737172571416885	24.73282901363657
24-25	21.456229515740606	21.537169503571484	32.364433189996774	24.642167790691136
26-27	21.41800105227151	21.56672771682136	35.4263911024257	21.58888012848143
28-29	21.444156265818577	21.530931551256028	35.50438574065458	21.520526442270818
30-31	21.435723113811157	21.41541077549734	35.50773143969674	21.641134670994767
32-33	21.41095119373005	21.454466243302562	35.5586346756908	21.57594788727659
34-35	21.470378313987183	21.563821211141384	35.455335030176236	21.510465444695196
36-37	21.456951779972815	21.47862521039877	35.40817281988203	21.656250189746377
38-39	21.62328255974753	21.611566856717143	34.7880384525608	21.97711213097453
40-41	22.576168609822364	22.420163925079915	31.826176471415373	23.177490993682348
42-43	22.72908965705372	22.552690585969092	32.013539247083585	22.7046805098936
44-45	22.780272354514686	23.0100127516371	31.629052000472996	22.58066289337522
46-47	22.56021132645636	23.496058241325944	31.47189988965185	22.471830542565847
48-49	22.49166725786793	23.7136067687112	31.13430750518819	22.660418468232677
50-51	22.511128640992336	23.949341625981276	30.88317876656705	22.65635096645934
52-53	22.509863650570153	24.021621096483855	30.74702971974002	22.721485533205975
54-55	22.656621548854904	23.817449424216615	30.486233767893868	23.039695259034616
56-57	22.72153039467831	23.733158807017183	30.420652735239827	23.124658063064675
58-59	22.858133334083252	23.762567155532654	30.201170358801548	23.178129151582542
60-61	23.045102839269628	23.78048258265049	29.808055264499078	23.366359313580805
62-63	23.19063591322088	23.870438497369324	29.40895401811051	23.52997157129928
64-65	23.34116486089878	24.07273094484525	28.925448800216795	23.660655394039175
66-67	23.471317420411104	24.144211305118347	28.471690675125743	23.91278059934481
68-69	23.5489584989197	24.352663977400294	28.212087841357192	23.88628968232282
70-71	23.75560381762053	24.579642570107083	27.847271145834572	23.817482466437816
72-73	23.938513457371673	24.569817905239137	27.58272240935209	23.908946228037095
74-75	23.910172684608906	24.680997662278312	27.470470084992083	23.9383595681207
76-77	23.95351889690175	24.79666882955678	27.30049384605657	23.949318427484904
78-79	24.031652750593867	24.635490468503736	27.2979298107079	24.034926970194498
80-81	24.05694705221958	24.67441932290985	27.251888388709038	24.01674523616153
82-83	24.161381084597732	24.733213336910815	27.124715790377902	23.980689788113555
84-85	24.156518375578038	24.727808746321102	26.98050822498604	24.135164653114817
86-87	24.173135343358105	24.779888162465465	26.944338808033535	24.1026376861429
88-89	24.286985206614037	24.829240833812865	26.809423982106633	24.07434997746647
90-91	24.414763750338743	24.703543872745772	26.693342008518385	24.188350368397103
92-93	24.370891842642255	24.840074147281022	26.585518731532602	24.203515278544117
94-95	24.4157058014685	24.899390533461858	26.43236907858819	24.25253458648145
96-97	24.41466764213005	24.85862449692283	26.335389212317583	24.391318648629543
98-99	24.391862024921966	24.910871537667624	26.318519622578513	24.3787468148319
100-101	24.497392361865867	25.130244492719005	25.873385729180292	24.49897741623483
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5270.0
1	3030.5
2	629.0
3	408.0
4	357.5
5	328.5
6	291.5
7	298.0
8	301.5
9	296.5
10	311.5
11	341.0
12	520.0
13	947.0
14	1496.5
15	2606.0
16	60562.5
17	75690.5
18	30868.0
19	24016.5
20	17941.5
21	15727.5
22	15834.0
23	16736.0
24	18333.0
25	20549.0
26	23562.5
27	27714.5
28	32509.0
29	38207.0
30	45314.0
31	52707.5
32	61052.0
33	71984.0
34	83575.0
35	95628.0
36	111307.0
37	126018.0
38	140767.5
39	157466.5
40	172761.0
41	185725.5
42	200328.5
43	217323.0
44	237036.0
45	259256.5
46	278202.0
47	298893.0
48	317122.5
49	325554.0
50	326780.0
51	328313.0
52	333768.0
53	338732.5
54	337249.0
55	325903.0
56	304957.5
57	277411.5
58	252264.0
59	227013.5
60	196234.0
61	165792.0
62	135769.5
63	104089.0
64	76517.0
65	55502.0
66	39194.0
67	28378.5
68	21239.5
69	16267.5
70	13056.0
71	10620.0
72	9209.5
73	7375.5
74	5403.5
75	4646.0
76	3805.0
77	2471.5
78	1525.0
79	984.5
80	562.5
81	365.5
82	267.5
83	164.0
84	98.5
85	51.0
86	18.0
87	3.5
88	0.5
89	0.5
90	0.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.0
96	0.5
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	6.391344585508521E-6
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	5.113075668406817E-5
28-29	0.0
30-31	0.003176498258997735
32-33	0.006378561896337504
34-35	0.0015403140451075536
36-37	0.0017576197610148431
38-39	0.0031445415360701924
40-41	0.003042280022702056
42-43	0.0024670590100062894
44-45	0.007094392489914458
46-47	0.0014380525317394172
48-49	3.1956722927542605E-4
50-51	0.003157324225241209
52-53	0.002109143713217812
54-55	5.432642897682243E-4
56-57	0.0
58-59	1.1504420253915337E-4
60-61	6.00786391037801E-4
62-63	0.0
64-65	0.0
66-67	5.113075668406817E-5
68-69	0.0063977359300940295
70-71	0.006531954166389709
72-73	0.0029400185093339196
74-75	0.0037389365825224847
76-77	0.03228268150140354
78-79	0.056537834203408376
80-81	0.04833773910020094
82-83	8.820055528001759E-4
84-85	0.0011248766470494996
86-87	0.0015978361463771303
88-89	0.003751719271693502
90-91	0.0
92-93	1.9174033756525563E-5
94-95	1.1504420253915337E-4
96-97	0.006046211977891061
98-99	0.0013038342954437383
100-101	5.7522101269576684E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	7823080.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.71853004585324
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.30635984917265	42.56763944824244
2	9.342390778839642	9.663494363910404
3	2.811098512881171	4.361576487008946
4	1.2394010014180732	2.563999917228048
5	0.7350603364440055	1.9008120047947143
6	0.48610671642339565	1.5084434891300669
7	0.3720589932186488	1.3469640953726014
8	0.29274230769379117	1.2112161468923002
9	0.24360819904549086	1.1339152165575372
>10	2.00563228834823	19.750550953330652
>50	0.13754790348181864	4.807701355800543
>100	0.0260242859003918	1.9419540656640417
>500	0.001021847434608118	0.3716584893992681
>1k	6.977745158548727E-4	0.7661970791798632
>5k	1.744436275318106E-4	0.5436766797122682
>10k+	7.476155465649026E-5	5.560200207776367
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	349944	4.473225379262388	No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT	66211	0.8463546327022095	No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15252	0.1949615752363519	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5565378342034083E-5	0.0	0.0	1.2782689171017042E-5	0.0
2	2.5565378342034083E-5	0.0	0.0	1.2782689171017042E-5	0.0
3	3.8348067513051125E-5	0.0	0.0	1.2782689171017042E-5	3.8348067513051125E-5
4	3.8348067513051125E-5	0.0	0.0	2.5565378342034083E-5	3.8348067513051125E-5
5	3.8348067513051125E-5	0.0	0.0	3.8348067513051125E-5	6.391344585508522E-5
6	3.8348067513051125E-5	0.0	0.0	3.8348067513051125E-5	7.669613502610225E-5
7	3.8348067513051125E-5	0.0	0.0	3.8348067513051125E-5	7.669613502610225E-5
8	5.113075668406817E-5	0.0	0.0	3.8348067513051125E-5	1.0226151336813633E-4
9	5.113075668406817E-5	0.0	0.0	3.8348067513051125E-5	1.0226151336813633E-4
10-11	5.113075668406817E-5	0.0	0.0	5.113075668406817E-5	1.2782689171017043E-4
12-13	6.391344585508522E-5	0.0	0.0	5.113075668406817E-5	1.5978361463771302E-4
14-15	6.391344585508522E-5	0.0	0.0	5.113075668406817E-5	2.0452302673627267E-4
16-17	7.030479044059374E-5	0.0	0.0	6.391344585508522E-5	2.2369706049279824E-4
18-19	8.94788241971193E-5	0.0	0.0	6.391344585508522E-5	2.6204512800584933E-4
20-21	1.0226151336813633E-4	6.391344585508521E-6	0.0	7.030479044059374E-5	3.1956722927542605E-4
22-23	1.1504420253915338E-4	1.2782689171017042E-5	0.0	7.669613502610225E-5	3.706979859594942E-4
24-25	1.4060958088118748E-4	1.2782689171017042E-5	0.0	8.94788241971193E-5	3.8987201971601976E-4
26-27	1.7256630380873007E-4	1.2782689171017042E-5	0.0	8.94788241971193E-5	4.0904605347254533E-4
28-29	1.9174033756525562E-4	1.2782689171017042E-5	0.0	8.94788241971193E-5	4.4100277640008796E-4
30-31	2.0452302673627267E-4	1.2782689171017042E-5	0.0	8.94788241971193E-5	4.921335330841561E-4
32-33	2.109143713217812E-4	1.2782689171017042E-5	0.0	8.94788241971193E-5	5.752210126957669E-4
34-35	2.3008840507830676E-4	1.2782689171017042E-5	0.0	8.94788241971193E-5	6.071777356233094E-4
36-37	2.4287109424932379E-4	1.2782689171017042E-5	0.0	8.94788241971193E-5	6.455258031363607E-4
38-39	2.748278171768664E-4	2.5565378342034083E-5	0.0	8.94788241971193E-5	6.774825260639032E-4
40-41	3.003931955189005E-4	2.5565378342034083E-5	0.0	8.94788241971193E-5	7.222219381624629E-4
42-43	3.06784540104409E-4	2.5565378342034083E-5	0.0	1.1504420253915338E-4	7.733526948465309E-4
44-45	3.4513260761746015E-4	2.5565378342034083E-5	0.0	1.1504420253915338E-4	8.628315190436504E-4
46-47	3.643066413739857E-4	2.5565378342034083E-5	0.0	1.214355471246619E-4	9.395276540697526E-4
48-49	3.770893305450027E-4	2.5565378342034083E-5	0.0	1.2782689171017043E-4	0.0010034410999248378
50-51	4.026547088870368E-4	3.195672292754261E-5	0.0	1.2782689171017043E-4	0.001041789167437889
52-53	4.857421884986476E-4	3.8348067513051125E-5	0.0	1.2782689171017043E-4	0.0010801372349509399
54-55	6.583084923073776E-4	3.8348067513051125E-5	0.0	1.4700092546669598E-4	0.001131267991635008
56-57	8.756142082146673E-4	3.8348067513051125E-5	0.0	1.533922700522045E-4	0.001188790092904585
58-59	0.0012974429508582298	3.8348067513051125E-5	0.0	1.533922700522045E-4	0.00125270353875967
60-61	0.0017320543826728093	3.8348067513051125E-5	0.0	1.533922700522045E-4	0.0012846602616872127
62-63	0.0022753186724410335	3.8348067513051125E-5	0.0	1.533922700522045E-4	0.001323008329200264
64-65	0.003131758846899175	3.8348067513051125E-5	0.0	1.533922700522045E-4	0.0013741390858843321
66-67	0.0044419844869284224	3.8348067513051125E-5	0.0	1.533922700522045E-4	0.0014124871533973832
68-69	0.006116516768331654	3.8348067513051125E-5	0.0	1.533922700522045E-4	0.0014316611871539088
70-71	0.00846853157579879	3.8348067513051125E-5	0.0	1.533922700522045E-4	0.0014827919438379768
72-73	0.011740900003579153	3.8348067513051125E-5	0.0	1.533922700522045E-4	0.0015147486667655193
74-75	0.016585539199394612	3.8348067513051125E-5	0.0	1.533922700522045E-4	0.001546705389693062
76-77	0.02247835890723347	3.8348067513051125E-5	0.0	1.533922700522045E-4	0.0016106188355481472
78-79	0.029726143667200132	3.8348067513051125E-5	0.0	1.533922700522045E-4	0.0016106188355481472
80-81	0.03933872592380494	3.8348067513051125E-5	0.0	1.533922700522045E-4	0.001655358247646707
82-83	0.052492113080781486	3.8348067513051125E-5	0.0	1.533922700522045E-4	0.0016873149705742495
84-85	0.06912239169227466	3.8348067513051125E-5	0.0	1.6617495922322155E-4	0.001719271693501792
86-87	0.08929347520413955	3.8348067513051125E-5	0.0	1.789576483942386E-4	0.0017448370718438262
88-89	0.11331214815648057	4.473941209855964E-5	0.0	1.789576483942386E-4	0.0017704024501858603
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	57030	0.0	94.31174	1
TCGACGG	57930	0.0	92.83832	2
TATCTCG	46735	0.0	91.836006	2
ATATCTC	47330	0.0	91.01264	1
ACGGCGC	59380	0.0	90.371346	5
GACGGCG	59485	0.0	90.267715	4
CGACGGC	59860	0.0	89.7419	3
CGGCGCG	59855	0.0	89.73352	6
ATCTCGA	48735	0.0	87.96976	3
TCTCGAG	49555	0.0	85.574875	4
CTCGAGG	51300	0.0	82.663994	5
TCGAGGG	51515	0.0	82.318985	6
CGAGGGC	52160	0.0	81.24694	7
AGGGCGC	52470	0.0	80.94795	9
GAGGGCG	53715	0.0	79.098274	8
CGCGCCG	110475	0.0	49.50324	9
GGCGCGC	112585	0.0	48.588135	7
GCGCGCC	112880	0.0	48.562138	8
TCCATAT	63355	0.0	42.376884	18-19
CCATATT	63695	0.0	42.16932	18-19
>>END_MODULE