FastQCFastQC Report
Tue 31 May 2016
SRR1051269_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051269_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7823080
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA458710.5863547349637227No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT176320.2253843754633725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG58500.089.839741
TCGACGG63350.083.227692
ACGGCGC78400.067.432715
CGACGGC85450.062.0915643
GACGGCG85700.061.744164
CGGCGCG86250.061.4605756
ATCTCGA40750.058.0725561
TCTCGAG53550.043.818652
CGCGCCG145300.043.5441789
GGCGCGC170750.037.081817
GCGCGCC171900.036.9442678
CTCGAGG71700.032.7264823
TCGAGGG73100.031.969754
CGAGGGC77450.030.419485
GGGCGCG78050.029.8204848
AGGGCGC81850.028.6681567
TCCATAT106500.025.37781118-19
CCATATT110000.025.2180618-19
GAGGGCG100950.023.2911226
GCCGGAT150300.023.26003512-13
Produced by FastQC (version 0.11.5)