##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051269_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7823080 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.266282589466044 33.0 31.0 34.0 31.0 34.0 2 32.53858761510811 34.0 31.0 34.0 31.0 34.0 3 32.66843544997622 34.0 31.0 34.0 31.0 34.0 4 36.19157211737576 37.0 35.0 37.0 35.0 37.0 5 36.100364562295155 37.0 35.0 37.0 35.0 37.0 6 36.10593998783088 37.0 35.0 37.0 35.0 37.0 7 36.07435741421537 37.0 35.0 37.0 35.0 37.0 8 36.08396846254928 37.0 35.0 37.0 35.0 37.0 9 37.86503359290714 39.0 38.0 39.0 35.0 39.0 10-11 37.83375211553506 39.0 38.0 39.0 35.0 39.0 12-13 37.80819459854686 39.0 38.0 39.0 35.0 39.0 14-15 39.1849843028577 41.0 39.0 41.0 36.0 41.0 16-17 39.132516285146004 40.5 38.5 41.0 36.0 41.0 18-19 39.07297797031348 40.0 38.5 41.0 35.5 41.0 20-21 39.03549637738589 40.0 38.5 41.0 35.0 41.0 22-23 38.93495912096003 40.0 38.0 41.0 35.0 41.0 24-25 38.84225643864053 40.0 38.0 41.0 35.0 41.0 26-27 38.67249721337376 40.0 38.0 41.0 34.5 41.0 28-29 38.468468429314285 40.0 38.0 41.0 34.0 41.0 30-31 38.284250512585835 40.0 38.0 41.0 34.0 41.0 32-33 38.44885984803939 40.0 38.0 41.0 34.0 41.0 34-35 38.41121029057609 40.0 38.0 41.0 34.0 41.0 36-37 38.332131526201955 40.0 38.0 41.0 34.0 41.0 38-39 38.210879985887914 40.0 38.0 41.0 33.5 41.0 40-41 37.99584696820178 40.0 37.0 41.0 33.0 41.0 42-43 37.880772918083416 40.0 37.0 41.0 33.0 41.0 44-45 37.78555415514094 40.0 37.0 41.0 33.0 41.0 46-47 37.63771845615793 40.0 36.5 41.0 32.5 41.0 48-49 37.52365813720427 40.0 36.0 41.0 32.0 41.0 50-51 37.5369795528104 39.0 36.0 41.0 32.0 41.0 52-53 37.369836624449704 39.0 35.5 41.0 32.0 41.0 54-55 37.04372523353973 39.0 35.0 41.0 31.0 41.0 56-57 36.7979607136831 39.0 35.0 40.5 31.0 41.0 58-59 36.514305107451285 38.0 35.0 40.0 31.0 41.0 60-61 36.20776414148903 37.5 35.0 40.0 31.0 41.0 62-63 35.82745465980151 37.0 35.0 40.0 30.0 41.0 64-65 35.4262674036313 36.5 34.0 39.0 30.0 41.0 66-67 35.00530098119923 36.0 34.0 39.0 29.0 41.0 68-69 34.60552876872025 35.0 34.0 38.5 29.0 40.0 70-71 34.17786364194154 35.0 34.0 37.5 29.0 39.5 72-73 33.78684507892032 35.0 33.0 37.0 28.5 39.0 74-75 33.43823289548362 35.0 33.0 36.5 28.0 39.0 76-77 32.88027573538811 35.0 32.5 36.0 27.0 37.5 78-79 32.79680164334252 35.0 33.0 35.5 27.0 37.0 80-81 32.56812924065713 35.0 33.0 35.0 27.0 37.0 82-83 32.2906555985622 35.0 33.0 35.0 26.0 36.0 84-85 32.06648116854231 35.0 32.0 35.0 26.0 36.0 86-87 31.775748362537517 35.0 32.0 35.0 25.0 36.0 88-89 31.602729104138014 35.0 32.0 35.0 25.0 35.0 90-91 31.36380696605429 34.0 32.0 35.0 24.5 35.0 92-93 31.082334067911873 34.0 31.5 35.0 24.0 35.0 94-95 30.788086533692614 34.0 31.0 35.0 23.0 35.0 96-97 30.524349680688424 34.0 31.0 35.0 20.5 35.0 98-99 30.123245000690265 34.0 31.0 35.0 17.5 35.0 100-101 28.350029336271646 33.0 28.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4163502378734094 1101 2 0.384851104166529 1101 3 0.3654196481001577 1101 4 0.22180689551522192 1101 5 0.23792961749503405 1101 6 0.21294247385451115 1101 7 0.27993778610483844 1101 8 0.2389280977527406 1101 9 0.31366038962541154 1101 10-11 0.30476232855609453 1101 12-13 0.3232063375140726 1101 14-15 0.43328136518016436 1101 16-17 0.3685829840906507 1101 18-19 0.3470914537953007 1101 20-21 0.36856346671857665 1101 22-23 0.3881917941040456 1101 24-25 0.369966119879237 1101 26-27 0.36701223852891474 1101 28-29 0.40660151247462295 1101 30-31 0.39481249196655455 1101 32-33 0.3369070605479223 1101 34-35 0.36674914805661274 1101 36-37 0.3577326188393428 1101 38-39 0.381054739844096 1101 40-41 0.37419528337378694 1101 42-43 0.37310017574868226 1101 44-45 0.3374039535191784 1101 46-47 0.25858526893667033 1101 48-49 0.30346314278825304 1101 50-51 0.44903245417683024 1101 52-53 0.43318486036134374 1101 54-55 0.5244417711292044 1101 56-57 0.4141469000055267 1101 58-59 0.3893322673059032 1101 60-61 0.2864442387845898 1101 62-63 0.36166610020645606 1101 64-65 0.37336563368211984 1101 66-67 0.24991029572781542 1101 68-69 0.21722140024687064 1101 70-71 0.2860340815083049 1101 72-73 0.21504278736999538 1101 74-75 0.22726219746057552 1101 76-77 0.17594392705380102 1101 78-79 0.20033610113674172 1101 80-81 0.17731270347357508 1101 82-83 0.010954994021084019 1101 84-85 0.04171141054285954 1101 86-87 0.23650835104154666 1101 88-89 0.23060143223692364 1101 90-91 0.15721593695466396 1101 92-93 0.03925653714840038 1101 94-95 0.06734186677990905 1101 96-97 0.11889942578991253 1101 98-99 0.21879069254486794 1101 100-101 0.23806363845083212 1102 1 0.3623377933791403 1102 2 0.34922519519606965 1102 3 0.28121353326035603 1102 4 0.18255788162723263 1102 5 0.21569481161109394 1102 6 0.22833731858322892 1102 7 0.23136459646084973 1102 8 0.21438566458191843 1102 9 0.2545654099488317 1102 10-11 0.27216568411387243 1102 12-13 0.23326126883483056 1102 14-15 0.34165375950177435 1102 16-17 0.34079822855366615 1102 18-19 0.3392732646520429 1102 20-21 0.3065342923672958 1102 22-23 0.3291574493855194 1102 24-25 0.34807474899447755 1102 26-27 0.35572188101603786 1102 28-29 0.43152099851913306 1102 30-31 0.3881373336361946 1102 32-33 0.32584253778862404 1102 34-35 0.39077838588626435 1102 36-37 0.40509846380999903 1102 38-39 0.39814930670813453 1102 40-41 0.45707902898791275 1102 42-43 0.4086959380298083 1102 44-45 0.3492413596605459 1102 46-47 0.2997215458941156 1102 48-49 0.3323089639318155 1102 50-51 0.4781021252207367 1102 52-53 0.4587547966554979 1102 54-55 0.5010483602584515 1102 56-57 0.34917249427243036 1102 58-59 0.3708858676856366 1102 60-61 0.3215955508851991 1102 62-63 0.3719848443700684 1102 64-65 0.3882005836276292 1102 66-67 0.34075875419663504 1102 68-69 0.29294552178591715 1102 70-71 0.4006264574636589 1102 72-73 0.34215642064179264 1102 74-75 0.3198256706664253 1102 76-77 0.2871100678359184 1102 78-79 0.32808720925721246 1102 80-81 0.39376891015207605 1102 82-83 0.29684529928892545 1102 84-85 0.2116852535008249 1102 86-87 0.49500385519205636 1102 88-89 0.5130916746984688 1102 90-91 0.39818196058797284 1102 92-93 0.34682096880755964 1102 94-95 0.4215494583505155 1102 96-97 0.4413367873302434 1102 98-99 0.5633307010559214 1102 100-101 0.629875737621262 1103 1 0.2699870601339498 1103 2 0.2666330281810474 1103 3 0.21423344747464057 1103 4 0.13343804759838918 1103 5 0.17602121653811764 1103 6 0.15018608927741184 1103 7 0.15186371592882608 1103 8 0.13534241745212228 1103 9 0.12808036976419857 1103 10-11 0.11955155311626697 1103 12-13 0.19895740235706683 1103 14-15 0.26431063399373755 1103 16-17 0.25115717406833227 1103 18-19 0.19763688659070766 1103 20-21 0.21600986698084768 1103 22-23 0.16804005631471597 1103 24-25 0.16254287321756777 1103 26-27 0.22583627608626955 1103 28-29 0.21617618222352064 1103 30-31 0.22085444500937967 1103 32-33 0.17551704659864242 1103 34-35 0.20756187027451745 1103 36-37 0.2669256101786175 1103 38-39 0.25738975184155066 1103 40-41 0.18867560758523183 1103 42-43 0.23724053823598013 1103 44-45 0.16863280431646643 1103 46-47 0.20567667978298232 1103 48-49 0.2681021490874471 1103 50-51 0.3035311393045106 1103 52-53 0.29665849416713286 1103 54-55 0.34560895070321607 1103 56-57 0.29662947901465486 1103 58-59 0.22320510142008487 1103 60-61 0.21513824027901052 1103 62-63 0.23805186385136068 1103 64-65 0.20317503929364022 1103 66-67 0.25714549380861484 1103 68-69 0.20208121244315436 1103 70-71 0.24646783474670997 1103 72-73 0.31538395884230397 1103 74-75 0.34507713137859497 1103 76-77 0.29843455082200876 1103 78-79 0.33725370817568034 1103 80-81 0.37875197256376225 1103 82-83 0.257435396489079 1103 84-85 0.3354648721955229 1103 86-87 0.4645686619310361 1103 88-89 0.44160642652539295 1103 90-91 0.3215853471507941 1103 92-93 0.2568614154774629 1103 94-95 0.36407385584826457 1103 96-97 0.4185762960155728 1103 98-99 0.43323435075837224 1103 100-101 0.5525161777064795 1104 1 0.4323254264722536 1104 2 0.4459070299716785 1104 3 0.33932406965372763 1104 4 0.22580670722994256 1104 5 0.26088761808154004 1104 6 0.22591782699766583 1104 7 0.26516323346529447 1104 8 0.26345626842611125 1104 9 0.30447952242611365 1104 10-11 0.29538703552686485 1104 12-13 0.28546304299349146 1104 14-15 0.3801692472678937 1104 16-17 0.33949008053451735 1104 18-19 0.39098404144725407 1104 20-21 0.4128478736512946 1104 22-23 0.40946551118100416 1104 24-25 0.4025357593032268 1104 26-27 0.45450564443793695 1104 28-29 0.42771631251893893 1104 30-31 0.42937608334180766 1104 32-33 0.39192244066320114 1104 34-35 0.4352762274667157 1104 36-37 0.41940775087794435 1104 38-39 0.46613013197320896 1104 40-41 0.43353974114203453 1104 42-43 0.479341386738092 1104 44-45 0.4018437323907236 1104 46-47 0.4553336821740359 1104 48-49 0.5171236449410586 1104 50-51 0.5450283646085836 1104 52-53 0.49891056163069436 1104 54-55 0.45046612107464057 1104 56-57 0.3757699344326184 1104 58-59 0.4549327872330835 1104 60-61 0.3218473122543699 1104 62-63 0.362836612013929 1104 64-65 0.4416672205642058 1104 66-67 0.31824730741792706 1104 68-69 0.31607233929918266 1104 70-71 0.3981175675154702 1104 72-73 0.38866817976786905 1104 74-75 0.4047507137008921 1104 76-77 0.31933530966502843 1104 78-79 0.33329094895265854 1104 80-81 0.43901695365916993 1104 82-83 0.30260007544513456 1104 84-85 0.30691838660957416 1104 86-87 0.49671330875276354 1104 88-89 0.5199163899869568 1104 90-91 0.4392088593841166 1104 92-93 0.2982827801000205 1104 94-95 0.5303501735801 1104 96-97 0.5774221176004986 1104 98-99 0.6913139012067688 1104 100-101 0.8437780873975989 1105 1 -0.09733222081388249 1105 2 0.0814215628789654 1105 3 0.024090436322914854 1105 4 0.04461224047673085 1105 5 0.02236927008603118 1105 6 0.0361187976570605 1105 7 0.0789574361451173 1105 8 -0.008442248568769628 1105 9 0.024916456734487724 1105 10-11 -5.072269060093504E-4 1105 12-13 0.03789490869193202 1105 14-15 0.05350272930058253 1105 16-17 0.06224781925918421 1105 18-19 -0.040841633243665854 1105 20-21 9.642481329805719E-4 1105 22-23 0.05181614973820814 1105 24-25 -0.004964697796758344 1105 26-27 0.015426724175021889 1105 28-29 0.015715673111699857 1105 30-31 0.012586409646104357 1105 32-33 0.09801763438025546 1105 34-35 0.06830233912066319 1105 36-37 0.07879295499514427 1105 38-39 0.10663643522789812 1105 40-41 0.1404274711796063 1105 42-43 0.11532733946623352 1105 44-45 0.097882604951252 1105 46-47 -0.1162999067600623 1105 48-49 0.11493535666942734 1105 50-51 0.11813369503592952 1105 52-53 0.16142677900988645 1105 54-55 0.18725806255139332 1105 56-57 0.04225151440362396 1105 58-59 0.15170713678803338 1105 60-61 0.1303763879127402 1105 62-63 0.16840556865538048 1105 64-65 0.21995005309337756 1105 66-67 0.23595452217653445 1105 68-69 0.1783815206137831 1105 70-71 0.11359576033740382 1105 72-73 0.1617466273428434 1105 74-75 0.20912657801591905 1105 76-77 0.16634461810598822 1105 78-79 0.27853624869791105 1105 80-81 0.31463594369775194 1105 82-83 0.28662841499534863 1105 84-85 0.2293906862581494 1105 86-87 0.4309230487832032 1105 88-89 0.3886960490818687 1105 90-91 0.36714676961889836 1105 92-93 0.23759952090096093 1105 94-95 0.35332414132512113 1105 96-97 0.48907139686489387 1105 98-99 0.6454254929426213 1105 100-101 0.8530091560582065 1106 1 -0.15030466744620696 1106 2 -0.03397579266781747 1106 3 -0.05053212131656437 1106 4 -0.014205259496016254 1106 5 -0.36871452052019293 1106 6 -0.27273091504118696 1106 7 -0.11936224236830384 1106 8 -0.3299556174194578 1106 9 -0.4740677653199157 1106 10-11 -0.10677919317548401 1106 12-13 -0.07632263621824364 1106 14-15 -0.25660300699015437 1106 16-17 -0.10544731487236447 1106 18-19 -0.20197384288585596 1106 20-21 -0.15277299408348455 1106 22-23 -0.15208864544588607 1106 24-25 -0.09633473420270633 1106 26-27 -0.07850655639805382 1106 28-29 -0.13910825441883645 1106 30-31 -0.1499839514308121 1106 32-33 -0.1493922756321453 1106 34-35 -0.09831178318992073 1106 36-37 -0.07919188757417572 1106 38-39 -0.08089151229263791 1106 40-41 -0.018261575164750354 1106 42-43 -0.14331759563057744 1106 44-45 -0.07642590515866488 1106 46-47 -0.17453249153891193 1106 48-49 -0.05320397518168818 1106 50-51 -0.028748203821152174 1106 52-53 -0.15572498228989673 1106 54-55 -0.11604369517874602 1106 56-57 -0.16883695415580746 1106 58-59 -0.08235444295758754 1106 60-61 -0.16587629506374668 1106 62-63 -0.12306127600277961 1106 64-65 -0.079777282958851 1106 66-67 -0.08730806076142983 1106 68-69 -0.1402286511383437 1106 70-71 -0.045870615879977095 1106 72-73 -0.04842206568062579 1106 74-75 -0.09532000703725885 1106 76-77 -0.1794591724806125 1106 78-79 -0.09928275850701596 1106 80-81 -0.08081920073308879 1106 82-83 -0.169051820465846 1106 84-85 -0.19200116915011023 1106 86-87 0.06565036324517948 1106 88-89 0.1207821011011383 1106 90-91 -0.017784375894148496 1106 92-93 -0.178777908957219 1106 94-95 -0.11551343616509158 1106 96-97 -0.015897926351343017 1106 98-99 0.14553898378180818 1106 100-101 0.11606921446214358 1107 1 -0.18123123253538864 1107 2 -0.1236260232872155 1107 3 -0.1871577905880386 1107 4 -0.11588619476302853 1107 5 -0.16368623417640293 1107 6 -0.15993071462361996 1107 7 -0.12598015660054074 1107 8 -0.12424710351767487 1107 9 -0.2479027399410043 1107 10-11 -0.2499665208012658 1107 12-13 -0.27361092440082757 1107 14-15 -0.2813287649417262 1107 16-17 -0.27985516822897694 1107 18-19 -0.2859668165679281 1107 20-21 -0.22753793358688057 1107 22-23 -0.28158336242330506 1107 24-25 -0.3706404445223015 1107 26-27 -0.4100538127688367 1107 28-29 -0.35927997116039023 1107 30-31 -0.45033331238703056 1107 32-33 -0.4638086781208344 1107 34-35 -0.3899055745246045 1107 36-37 -0.3701832884086329 1107 38-39 -0.2544157607762614 1107 40-41 -0.2981710867036682 1107 42-43 -0.28179225261689567 1107 44-45 -0.3514773933764346 1107 46-47 -0.3793018657863598 1107 48-49 -0.26886685519479414 1107 50-51 -0.1998025708693092 1107 52-53 -0.23066014196388096 1107 54-55 -0.29698897627091014 1107 56-57 -0.43549727477840605 1107 58-59 -0.3490996653158476 1107 60-61 -0.3813869557232863 1107 62-63 -0.3183048652397886 1107 64-65 -0.30910227182544503 1107 66-67 -0.3474235904838636 1107 68-69 -0.32556713934692993 1107 70-71 -0.2805097955463083 1107 72-73 -0.2795838072624761 1107 74-75 -0.2504097624327244 1107 76-77 -0.35380266796621385 1107 78-79 -0.32955551802221805 1107 80-81 -0.217492845092913 1107 82-83 -0.22735660719073536 1107 84-85 -0.3061700700094647 1107 86-87 -0.1753969609943944 1107 88-89 -0.15682584742840788 1107 90-91 -0.3321730870915047 1107 92-93 -0.39167831815884924 1107 94-95 -0.4055092877909594 1107 96-97 -0.44971861114308354 1107 98-99 -0.36960942779755257 1107 100-101 -0.23269214255062565 1108 1 -0.37141057286597245 1108 2 -0.2814086384147316 1108 3 -0.30795647874546006 1108 4 -0.20879792869753544 1108 5 -0.3757841069332031 1108 6 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2305 8 -0.07211669207256932 2305 9 -0.005867649481238857 2305 10-11 0.028148812798207246 2305 12-13 0.02069371560424571 2305 14-15 0.12203768319034225 2305 16-17 0.05744065844922375 2305 18-19 0.13486561450795165 2305 20-21 0.12712781305119591 2305 22-23 0.14999447662312804 2305 24-25 0.06904504363559028 2305 26-27 0.1800256287013866 2305 28-29 0.1178554934393361 2305 30-31 0.12267621886924474 2305 32-33 0.11202202543135087 2305 34-35 0.10866439932047456 2305 36-37 0.1377216935280572 2305 38-39 0.13914304186767623 2305 40-41 0.22854983456801392 2305 42-43 0.2771423378174518 2305 44-45 0.3010567391665404 2305 46-47 0.2927660982402642 2305 48-49 0.24090215323899855 2305 50-51 0.13895343162968032 2305 52-53 0.22977510135957857 2305 54-55 0.25091434965317916 2305 56-57 0.2749682761346648 2305 58-59 0.3079289427389966 2305 60-61 0.4109915281573393 2305 62-63 0.3536719047131527 2305 64-65 0.3177986533501027 2305 66-67 0.4709732079177016 2305 68-69 0.4880558788134408 2305 70-71 0.5010384226763023 2305 72-73 0.47765890974429936 2305 74-75 0.41983496639286955 2305 76-77 0.3965643395074423 2305 78-79 0.47185985119027407 2305 80-81 0.5558868723583572 2305 82-83 0.5625128847958152 2305 84-85 0.5727012145491628 2305 86-87 0.7184573258680906 2305 88-89 0.6722406721710392 2305 90-91 0.6884970421165129 2305 92-93 0.8622829331225788 2305 94-95 0.9818739597984241 2305 96-97 0.9356365927865866 2305 98-99 1.0208888177021365 2305 100-101 1.2236766277406517 2306 1 -0.5493379977602082 2306 2 -0.9625769822281676 2306 3 -0.7371008813697664 2306 4 -0.35557259495703875 2306 5 -0.1958948044070894 2306 6 -0.1920590234434556 2306 7 -0.06860641067348183 2306 8 -0.04129635200046522 2306 9 -0.03230460804832802 2306 10-11 -0.3262648554334717 2306 12-13 -0.2345870375396757 2306 14-15 -0.11297065395792316 2306 16-17 -0.19054743164083732 2306 18-19 -0.1309447349308428 2306 20-21 -0.12599492630078402 2306 22-23 -0.07062103652542362 2306 24-25 -0.10929070249203932 2306 26-27 -0.12071554137642693 2306 28-29 -0.07218476525929418 2306 30-31 -0.038238077470751364 2306 32-33 -0.06583226348715954 2306 34-35 -0.17665824188259194 2306 36-37 -0.167297425981225 2306 38-39 -0.0795634701065282 2306 40-41 -0.17011782640378925 2306 42-43 -0.05121172040541211 2306 44-45 -0.1672259539022818 2306 46-47 -0.09571000717729561 2306 48-49 -0.030803070662678067 2306 50-51 -0.025840249350785882 2306 52-53 -0.01307513548746897 2306 54-55 -0.09741544529141777 2306 56-57 -0.05143598920403036 2306 58-59 -0.14937171476155697 2306 60-61 -0.22310203867088063 2306 62-63 -0.013620736199513317 2306 64-65 -0.07698570680399541 2306 66-67 -0.046450975260661664 2306 68-69 0.008530093330435307 2306 70-71 0.01575492449408955 2306 72-73 -0.02349863255216178 2306 74-75 -0.03814683368910465 2306 76-77 -0.01955921269652805 2306 78-79 0.043128225882135496 2306 80-81 0.2145129329982609 2306 82-83 0.2124707990526602 2306 84-85 0.09308194025135208 2306 86-87 0.29769437117093744 2306 88-89 0.11857588556874532 2306 90-91 0.200973851162221 2306 92-93 0.2183333434685366 2306 94-95 0.37203079761209423 2306 96-97 0.19242183592686857 2306 98-99 0.36905385373354704 2306 100-101 0.7634031535838197 2307 1 -1.6108140373750572 2307 2 -2.1636921940765603 2307 3 -1.747020690571997 2307 4 -0.8678320373281423 2307 5 -0.5071305659540641 2307 6 -0.4173561396647898 2307 7 -0.35501942120903607 2307 8 -0.5056256824051104 2307 9 -0.43933761532346693 2307 10-11 -0.36028722131743507 2307 12-13 -0.36090892212190084 2307 14-15 -0.6914136579871482 2307 16-17 -0.5592887343552491 2307 18-19 -0.40398591333176626 2307 20-21 -0.43372748827709273 2307 22-23 -0.5207842661327433 2307 24-25 -0.42631868117114635 2307 26-27 -0.4284032192234193 2307 28-29 -0.41556687684051496 2307 30-31 -0.7724734661114496 2307 32-33 -0.484422964768342 2307 34-35 -0.48566870436798837 2307 36-37 -0.39757381232775657 2307 38-39 -0.38578234590244875 2307 40-41 -0.3229748261387826 2307 42-43 -0.31222537464520883 2307 44-45 -0.29067238929921047 2307 46-47 -0.35861259259576883 2307 48-49 -0.3112081128402977 2307 50-51 -0.26523886814656805 2307 52-53 -0.3119285291484175 2307 54-55 -0.2967985224698424 2307 56-57 -0.1889923725158127 2307 58-59 -0.38840357207612897 2307 60-61 -0.37264010925790814 2307 62-63 -0.22203148722806532 2307 64-65 -0.2197686852217302 2307 66-67 -0.2681283518572215 2307 68-69 -0.11549698743703374 2307 70-71 -0.20168290781649034 2307 72-73 -0.2578715252110868 2307 74-75 -0.22548165678181675 2307 76-77 -0.20146906315601143 2307 78-79 -0.23642708359001574 2307 80-81 -0.20612236726913125 2307 82-83 -0.19780018459980653 2307 84-85 -0.20183137179846256 2307 86-87 -0.16897625557693274 2307 88-89 -0.25235954579695274 2307 90-91 -0.24725687276243136 2307 92-93 -0.13661870520655484 2307 94-95 -0.060982684387912656 2307 96-97 -0.07080269655739357 2307 98-99 -0.11824674282452108 2307 100-101 0.10630311888854038 2308 1 -1.2426565720392162 2308 2 -1.2248300065062843 2308 3 -0.7643136768188832 2308 4 -0.47771181000986473 2308 5 -0.6421660911899707 2308 6 -0.5441317476513845 2308 7 -1.439469791563539 2308 8 -0.5694663143736136 2308 9 -0.5494545803964499 2308 10-11 -0.8156260510092181 2308 12-13 -0.8700167897528317 2308 14-15 -0.6549336858524768 2308 16-17 -0.8372190437931124 2308 18-19 -0.6090151735431917 2308 20-21 -0.6890612646327483 2308 22-23 -0.5190929946741889 2308 24-25 -0.5220851633933492 2308 26-27 -0.48315673345478416 2308 28-29 -1.3457134580187997 2308 30-31 -0.6592783172228422 2308 32-33 -0.5089947241072608 2308 34-35 -1.136885345272951 2308 36-37 -0.6653351242724526 2308 38-39 -0.44743620042724785 2308 40-41 -0.9474987162169342 2308 42-43 -0.38931540452361446 2308 44-45 -0.5066541648582827 2308 46-47 -0.46900002800961005 2308 48-49 -0.36108511591190506 2308 50-51 -0.44636806023230946 2308 52-53 -0.5486999172987552 2308 54-55 -0.9802990999900629 2308 56-57 -1.7019022720083683 2308 58-59 -1.1073121065731897 2308 60-61 -0.7054725979033449 2308 62-63 -0.4156941391806086 2308 64-65 -0.2467699808130206 2308 66-67 -0.3029026341781247 2308 68-69 -0.2102447268679839 2308 70-71 -0.10033322374901132 2308 72-73 -0.2071429776171385 2308 74-75 -0.07228576951910526 2308 76-77 -1.1066263731497443 2308 78-79 -0.6060052777106648 2308 80-81 -0.1192472233809383 2308 82-83 0.02642483306733112 2308 84-85 0.09902926770018183 2308 86-87 -0.24955048018776793 2308 88-89 -0.24448081481808615 2308 90-91 -0.8512352158721121 2308 92-93 0.01858347930574311 2308 94-95 0.2634756653447603 2308 96-97 0.12546245404370637 2308 98-99 -0.3676005457386786 2308 100-101 0.4017099214968205 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 15.0 12 111.0 13 637.0 14 2333.0 15 5559.0 16 9259.0 17 13922.0 18 21084.0 19 30632.0 20 30152.0 21 30503.0 22 32558.0 23 36900.0 24 43050.0 25 52296.0 26 65132.0 27 81373.0 28 101471.0 29 126044.0 30 155404.0 31 193344.0 32 243529.0 33 319154.0 34 447435.0 35 700373.0 36 1180479.0 37 2056334.0 38 1644277.0 39 199629.0 40 91.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 25.178655123134913 27.97222390956146 23.160873054187032 23.688247913116594 2 25.863083874353944 26.914058670158582 25.360682181299993 21.862175274187486 3 24.64527298659936 25.217593938329486 25.19642574553327 24.940707329537886 4 26.097324402146775 25.266308359092605 24.818044671442454 23.818322567318166 5 25.616573012164007 26.29172908879879 24.305196930109368 23.78650096892784 6 25.492517013759286 25.068387387064938 23.60125423746146 25.837841361714315 7 26.164285677763743 25.288863209886642 24.184119298281495 24.362731814068116 8 25.492197446530014 25.094310680703764 24.42035617685106 24.993135695915164 9 24.735244941889896 24.614218440818707 24.716531084943526 25.934005532347875 10-11 25.155827372339285 25.184358334568994 24.46936500713274 25.190449285958984 12-13 25.15711842394556 25.108365247447296 24.584332513536868 25.15018381507028 14-15 24.98541495165587 25.181175444965408 24.689700220373563 25.14370938300516 16-17 25.090936050762615 25.530794776481898 24.616129452849773 24.762139719905715 18-19 24.586070959264124 25.175595801142258 25.096189736011905 25.14214350358171 20-21 24.41289108637519 25.35593397996697 24.987715835706652 25.24345909795119 22-23 24.496310915905244 25.39076680795799 25.445470326265358 24.667451949871406 24-25 24.51671208782219 24.94697740531862 25.948290187496486 24.588020319362705 26-27 24.46999135890212 24.966880052357894 26.036017783277178 24.527110805462808 28-29 24.531770095665646 25.106614019030864 25.935085669582826 24.426530215720664 30-31 24.500267158203673 24.99077728976311 25.969803453371306 24.53915209866191 32-33 24.447199824110196 25.050210403063755 25.991649069164573 24.510940703661475 34-35 24.51864227388701 25.155737893515084 25.907238581223762 24.41838125137414 36-37 24.52863194547416 25.07341098390915 25.855385602601533 24.542571468015158 38-39 24.49610000153392 25.122343118055806 25.854695337386296 24.526861543023976 40-41 24.650335929071414 25.280177372594935 25.4557284343251 24.61375826400855 42-43 24.712645147435534 25.26208986741795 25.411794331644316 24.6134706535022 44-45 24.59707685464037 25.348264366464356 25.492977190569444 24.561681588325825 46-47 24.65411321372145 25.495194987140614 25.351293863797892 24.499397935340046 48-49 24.632363468096965 25.45275645909284 25.33354509988393 24.581334972926264 50-51 24.548234199317918 25.476877393558546 25.426999340413236 24.547889066710297 52-53 24.59292887200438 25.578231335995543 25.35818373326107 24.470656058739014 54-55 24.59409209671894 25.47543294968222 25.34197528339222 24.58849967020662 56-57 24.555667333070865 25.532731353891307 25.437941322343626 24.473659990694202 58-59 24.6051810795748 25.60285079533892 25.370697985959495 24.42127013912679 60-61 24.593011959483988 25.553356222868747 25.303422692852433 24.55020912479484 62-63 24.553136360615 25.621679696487824 25.32219407190007 24.5029898709971 64-65 24.58743231566084 25.745396953629516 25.228113479601383 24.43905725110826 66-67 24.600969183492946 25.658723929705435 25.173531396841142 24.566775489960477 68-69 24.581999672763157 25.74154936418904 25.195440926080266 24.481010036967536 70-71 24.661373781170585 25.83970763433328 25.075852477540817 24.423066106955314 72-73 24.663808883457666 25.822105871344792 25.013134213123223 24.500951032074322 74-75 24.604196812508626 25.867522765969415 25.088884429150664 24.439395992371292 76-77 24.611316770376884 26.012107763182783 24.952505918385086 24.42406954805524 78-79 24.62935314479719 25.897645173000917 25.001412487153395 24.471589195048498 80-81 24.547882675365713 25.958535512867055 25.065006365779208 24.428575445988024 82-83 24.619254820352086 26.067795548556322 24.956481334717274 24.356468296374317 84-85 24.59108816476375 26.038267536571276 24.916701606017067 24.45394269264791 86-87 24.525084749229205 26.12123358063576 24.96252115535058 24.391160514784456 88-89 24.63754684855581 26.200901690894124 24.843348144209187 24.318203316340878 90-91 24.637131411157753 26.12003839919827 24.830444019491043 24.412386170152935 92-93 24.54409899937109 26.213850555024365 24.833575778337945 24.4084746672666 94-95 24.61584980697179 26.32595003029178 24.746904336073793 24.311295826662633 96-97 24.578804000470402 26.26818337534577 24.74405860607331 24.408954018110514 98-99 24.534486417114486 26.344304289359176 24.751280825454934 24.3699284680714 100-101 24.651649305855152 26.48361342126291 24.388696573305726 24.476040699576217 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 43714.0 1 24567.0 2 3865.5 3 2055.5 4 1665.0 5 1488.5 6 1411.5 7 1346.0 8 1342.5 9 1314.0 10 1326.5 11 1403.0 12 1660.5 13 2264.5 14 2959.5 15 3325.5 16 8475.0 17 9406.5 18 4950.5 19 4542.5 20 4421.0 21 4814.5 22 5735.5 23 6992.0 24 8583.5 25 10710.5 26 13398.0 27 16695.5 28 20390.0 29 25266.5 30 31574.5 31 37358.0 32 43583.0 33 53191.0 34 63336.0 35 73462.0 36 89625.5 37 105844.5 38 122105.5 39 141237.5 40 159974.5 41 175769.0 42 192597.0 43 211668.0 44 233136.5 45 257553.5 46 280151.5 47 306291.5 48 329144.0 49 342320.5 50 346788.5 51 349769.5 52 357556.5 53 365708.0 54 366632.5 55 357147.0 56 335988.0 57 306798.0 58 279686.5 59 253549.0 60 222601.5 61 189200.0 62 155352.0 63 119373.5 64 87484.5 65 63515.0 66 45220.0 67 33097.5 68 25059.0 69 19249.0 70 15594.0 71 12763.0 72 11090.0 73 9036.5 74 6817.0 75 5803.0 76 4858.5 77 3324.5 78 2001.0 79 1246.5 80 775.5 81 554.5 82 462.0 83 377.5 84 238.5 85 134.5 86 63.5 87 5.5 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04352505662731303 2 7.286132827479714E-4 3 2.812191617623749E-4 4 7.669613502610225E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 6.391344585508521E-6 96-97 0.0 98-99 0.0 100-101 1.2782689171017042E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7823080.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.49798615676783 #Duplication Level Percentage of deduplicated Percentage of total 1 81.92762714914792 43.82963063081953 2 9.454802494282726 10.116257859082225 3 2.787367484075712 4.473556412307217 4 1.297225931241358 2.7759589964700173 5 0.7417197565746126 1.984025663471492 6 0.483119091277221 1.5507539074331418 7 0.3757042164038762 1.406959327774971 8 0.29397899170397285 1.2581827222847766 9 0.2463397943167752 1.1860814615597888 >10 2.217586637789655 22.814275550987592 >50 0.1447752591009396 5.232441582722194 >100 0.02939452495835905 2.1120858721386195 >500 9.565025725742384E-5 0.03972093528816653 >1k 1.6737564443961635E-4 0.23534353378505363 >5k 4.7821612602801116E-5 0.1724078783121883 >10k+ 4.7821612602801116E-5 0.8123176655630577 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 45871 0.5863547349637227 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17632 0.2253843754633725 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.2782689171017042E-5 0.0 0.0 0.0 1.2782689171017042E-5 2 1.2782689171017042E-5 0.0 0.0 0.0 1.2782689171017042E-5 3 2.5565378342034083E-5 0.0 0.0 1.2782689171017042E-5 3.8348067513051125E-5 4 2.5565378342034083E-5 0.0 0.0 1.2782689171017042E-5 3.8348067513051125E-5 5 3.8348067513051125E-5 0.0 0.0 1.2782689171017042E-5 5.113075668406817E-5 6 3.8348067513051125E-5 0.0 0.0 3.8348067513051125E-5 7.669613502610225E-5 7 3.8348067513051125E-5 0.0 0.0 5.113075668406817E-5 1.2782689171017043E-4 8 5.113075668406817E-5 0.0 0.0 5.113075668406817E-5 1.6617495922322155E-4 9 5.113075668406817E-5 0.0 0.0 5.113075668406817E-5 1.9174033756525562E-4 10-11 5.113075668406817E-5 0.0 0.0 5.113075668406817E-5 2.109143713217812E-4 12-13 6.391344585508522E-5 0.0 0.0 5.113075668406817E-5 2.364797496638153E-4 14-15 6.391344585508522E-5 0.0 0.0 5.113075668406817E-5 2.748278171768664E-4 16-17 7.030479044059374E-5 0.0 0.0 6.391344585508522E-5 3.003931955189005E-4 18-19 8.94788241971193E-5 0.0 0.0 6.391344585508522E-5 3.1956722927542605E-4 20-21 1.0226151336813633E-4 1.9174033756525563E-5 0.0 6.391344585508522E-5 3.8987201971601976E-4 22-23 1.1504420253915338E-4 2.5565378342034083E-5 0.0 6.391344585508522E-5 4.218287426435624E-4 24-25 1.4060958088118748E-4 2.5565378342034083E-5 0.0 6.391344585508522E-5 4.793508439131391E-4 26-27 1.5978361463771302E-4 2.5565378342034083E-5 0.0 6.391344585508522E-5 5.432642897682243E-4 28-29 1.789576483942386E-4 2.5565378342034083E-5 0.0 6.391344585508522E-5 5.880037018667839E-4 30-31 1.9174033756525562E-4 2.5565378342034083E-5 0.0 6.391344585508522E-5 6.455258031363607E-4 32-33 1.9813168215076414E-4 2.5565378342034083E-5 0.0 7.669613502610225E-5 7.158305935769544E-4 34-35 2.1730571590728972E-4 2.5565378342034083E-5 0.0 7.669613502610225E-5 7.669613502610225E-4 36-37 2.3008840507830676E-4 2.5565378342034083E-5 0.0 7.669613502610225E-5 8.180921069450907E-4 38-39 2.5565378342034086E-4 3.195672292754261E-5 0.0 7.669613502610225E-5 8.883968973856845E-4 40-41 2.748278171768664E-4 4.473941209855964E-5 0.0 7.669613502610225E-5 9.587016878262781E-4 42-43 2.812191617623749E-4 5.113075668406817E-5 0.0 7.669613502610225E-5 0.0010737458903654316 44-45 3.1956722927542605E-4 5.113075668406817E-5 0.0 7.669613502610225E-5 0.0011184853024639911 46-47 3.387412630319516E-4 5.113075668406817E-5 0.0 9.587016878262781E-5 0.0011440506808060252 48-49 3.5791529678847714E-4 5.113075668406817E-5 0.0 1.0226151336813633E-4 0.001201572782075602 50-51 3.770893305450027E-4 5.113075668406817E-5 0.0 1.0226151336813633E-4 0.001227138160417636 52-53 4.473941209855965E-4 5.752210126957669E-5 0.0 1.0226151336813633E-4 0.0012463121941741616 54-55 6.13569080208818E-4 6.391344585508522E-5 0.0 1.0226151336813633E-4 0.0012782689171017042 56-57 8.372661407016163E-4 6.391344585508522E-5 0.0 1.2782689171017043E-4 0.0013741390858843321 58-59 0.0012718775725161957 7.030479044059374E-5 0.0 1.2782689171017043E-4 0.0014252698425684003 60-61 0.001719271693501792 8.308747961161077E-5 0.0 1.2782689171017043E-4 0.0014572265654959429 62-63 0.0022497532940989992 8.94788241971193E-5 0.0 1.2782689171017043E-4 0.0015019659775945024 64-65 0.003106193468557141 8.94788241971193E-5 0.0 1.3421823629567895E-4 0.0015850534572061131 66-67 0.004397245074829862 1.0226151336813633E-4 0.0 1.4060958088118745E-4 0.0016234015247191644 68-69 0.006046211977891061 1.1504420253915338E-4 0.0 1.4700092546669598E-4 0.0016489669030611983 70-71 0.008404618129943704 1.1504420253915338E-4 0.0 1.533922700522045E-4 0.001680923625988741 72-73 0.011676986557724069 1.1504420253915338E-4 0.0 1.5978361463771302E-4 0.001732054382672809 74-75 0.01646410365226995 1.1504420253915338E-4 0.0 1.7256630380873007E-4 0.0017512284164293347 76-77 0.022395271427621856 1.1504420253915338E-4 0.0 1.853489929797471E-4 0.0017704024501858603 78-79 0.0297005782888581 1.1504420253915338E-4 0.0 1.9813168215076414E-4 0.0018023591731134029 80-81 0.03935150861297596 1.1504420253915338E-4 0.0 2.0452302673627267E-4 0.0018407072406264541 82-83 0.0523578948444858 1.1504420253915338E-4 0.0 2.1730571590728972E-4 0.0018790553081395051 84-85 0.06870056294963109 1.1504420253915338E-4 0.0 2.1730571590728972E-4 0.0019110120310670477 86-87 0.08853290519846403 1.1504420253915338E-4 0.0 2.1730571590728972E-4 0.0019429687539945905 88-89 0.11241096856992386 1.1504420253915338E-4 0.0 2.3008840507830676E-4 0.00198770816609315 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 5850 0.0 89.83974 1 TCGACGG 6335 0.0 83.22769 2 ACGGCGC 7840 0.0 67.43271 5 CGACGGC 8545 0.0 62.091564 3 GACGGCG 8570 0.0 61.74416 4 CGGCGCG 8625 0.0 61.460575 6 ATCTCGA 4075 0.0 58.072556 1 TCTCGAG 5355 0.0 43.81865 2 CGCGCCG 14530 0.0 43.544178 9 GGCGCGC 17075 0.0 37.08181 7 GCGCGCC 17190 0.0 36.944267 8 CTCGAGG 7170 0.0 32.726482 3 TCGAGGG 7310 0.0 31.96975 4 CGAGGGC 7745 0.0 30.41948 5 GGGCGCG 7805 0.0 29.820484 8 AGGGCGC 8185 0.0 28.668156 7 TCCATAT 10650 0.0 25.377811 18-19 CCATATT 11000 0.0 25.21806 18-19 GAGGGCG 10095 0.0 23.291122 6 GCCGGAT 15030 0.0 23.260035 12-13 >>END_MODULE