FastQCFastQC Report
Tue 31 May 2016
SRR1051268_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051268_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12139190
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT8330646.862599563891825No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1140270.939329559880025No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT595440.4905104871082832No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT298970.24628496629511523No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT258550.2129878517429911No Hit
GTCGACGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166190.13690369785792955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG953550.094.348381
TCGACGG973400.092.439032
ATATCTC1086200.092.2522661
CGACGGC994400.090.3592453
TATCTCG1110850.090.0725862
ACGGCGC999650.089.8455055
GACGGCG1003950.089.550964
CGGCGCG1008850.088.99646
ATCTCGA1159900.086.190233
TCTCGAG1181150.083.793814
CTCGAGG1211200.081.738075
TCGAGGG1221100.081.1124956
CGAGGGC1242600.079.629987
AGGGCGC1255050.079.001819
GAGGGCG1271650.078.015358
CTCGACG23650.041.376885
CGCGCCG2241450.040.838469
CCATATT1084900.040.7969718-19
TCCATAT1088400.040.56322518-19
ATCCATA1092300.040.5097116-17
Produced by FastQC (version 0.11.5)