##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051268_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12139190 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.35638778205136 34.0 31.0 34.0 31.0 34.0 2 32.54153324892353 34.0 31.0 34.0 31.0 34.0 3 32.62616756142708 34.0 31.0 34.0 31.0 34.0 4 36.07743440872085 37.0 37.0 37.0 35.0 37.0 5 36.04254666085628 37.0 37.0 37.0 35.0 37.0 6 36.073553424899025 37.0 37.0 37.0 35.0 37.0 7 36.07134948872206 37.0 37.0 37.0 35.0 37.0 8 36.040405990844526 37.0 37.0 37.0 35.0 37.0 9 37.71362636222021 39.0 38.0 39.0 35.0 39.0 10-11 37.76930726020434 39.0 38.0 39.0 35.0 39.0 12-13 37.70524338938595 39.0 38.0 39.0 35.0 39.0 14-15 39.192621707049646 41.0 39.0 41.0 36.0 41.0 16-17 39.14798582112975 41.0 39.0 41.0 36.0 41.0 18-19 39.10872059832658 41.0 39.0 41.0 36.0 41.0 20-21 39.064998282422465 40.5 39.0 41.0 35.5 41.0 22-23 38.97449784540814 41.0 39.0 41.0 35.0 41.0 24-25 38.94977486141991 41.0 39.0 41.0 35.0 41.0 26-27 38.936774323492756 41.0 39.0 41.0 35.0 41.0 28-29 38.793244442174476 40.5 38.5 41.0 35.0 41.0 30-31 38.334642179585295 40.0 37.0 41.0 35.0 41.0 32-33 37.90508135221543 40.0 36.0 41.0 34.5 41.0 34-35 37.72937506538739 40.0 35.0 41.0 34.0 41.0 36-37 37.60045802067519 39.5 35.0 41.0 34.0 41.0 38-39 37.32898405906819 39.0 35.0 41.0 33.0 41.0 40-41 36.48051999350863 39.0 35.0 41.0 30.0 41.0 42-43 36.44306708272957 39.0 35.0 41.0 30.0 41.0 44-45 36.337855697126415 39.0 35.0 41.0 29.5 41.0 46-47 36.476294835157866 39.0 35.0 41.0 30.0 41.0 48-49 36.49683422040515 39.0 35.0 41.0 29.5 41.0 50-51 36.13730755511694 38.5 35.0 40.5 29.0 41.0 52-53 36.11906420444856 39.0 35.0 40.0 29.5 41.0 54-55 36.08150020718021 39.0 35.0 41.0 30.0 41.0 56-57 35.756441739522984 38.0 35.0 41.0 29.0 41.0 58-59 35.42868432737275 37.5 35.0 40.0 29.0 41.0 60-61 35.05986058377866 37.0 35.0 40.0 28.0 41.0 62-63 34.81536317497296 37.0 35.0 40.0 28.0 41.0 64-65 34.522182863930794 36.0 35.0 39.5 27.5 41.0 66-67 34.17285078328949 35.5 34.0 39.0 27.0 41.0 68-69 33.877893665063326 35.0 34.0 39.0 26.5 41.0 70-71 33.54886742855166 35.0 34.0 37.5 26.0 40.0 72-73 33.20230649656196 35.0 34.0 37.0 26.0 39.0 74-75 32.88000373171521 35.0 34.0 36.5 26.0 39.0 76-77 32.56947316913237 35.0 34.0 36.0 25.0 39.0 78-79 32.29221842643537 35.0 34.0 36.0 25.0 37.0 80-81 32.02000026360902 35.0 33.5 35.0 25.0 37.0 82-83 31.79999736390978 35.0 33.0 35.0 24.5 36.5 84-85 31.60433542106187 35.0 33.0 35.0 24.0 36.0 86-87 31.426153021741975 35.0 33.0 35.0 23.5 36.0 88-89 31.263840626928157 35.0 33.0 35.0 22.5 35.5 90-91 31.094090956645378 35.0 33.0 35.0 20.0 35.0 92-93 30.910807228488885 35.0 33.0 35.0 19.0 35.0 94-95 30.74321989358433 35.0 33.0 35.0 17.5 35.0 96-97 30.53413617383038 35.0 33.0 35.0 6.0 35.0 98-99 30.293889419310513 35.0 33.0 35.0 2.0 35.0 100-101 29.460363335609706 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4261235659932652 1101 2 0.4563309414700498 1101 3 0.4535384117554173 1101 4 0.31690296080589775 1101 5 0.3249072493997218 1101 6 0.3299331912375152 1101 7 0.36146726239545046 1101 8 0.27515004826685896 1101 9 0.43054797819657864 1101 10-11 0.3913899957882023 1101 12-13 0.44528764486076966 1101 14-15 0.5762319386645345 1101 16-17 0.48971140974478544 1101 18-19 0.5908533894024757 1101 20-21 0.6324601923640927 1101 22-23 0.6444698446293131 1101 24-25 0.6776559919944418 1101 26-27 0.610167427888392 1101 28-29 0.6111010245075121 1101 30-31 0.6077286959908079 1101 32-33 0.577114404235914 1101 34-35 0.5388622922750912 1101 36-37 0.5782930668792474 1101 38-39 0.5505911487219137 1101 40-41 0.4610200097749342 1101 42-43 0.4458474683806415 1101 44-45 0.3502025627723597 1101 46-47 0.3409284852774448 1101 48-49 0.26000753221915573 1101 50-51 0.19296051719602048 1101 52-53 0.14541410740913108 1101 54-55 0.1301243633702498 1101 56-57 0.17401615421101724 1101 58-59 0.09847986837949918 1101 60-61 0.044273333494381006 1101 62-63 0.1334121092912568 1101 64-65 0.03439535240904945 1101 66-67 0.1106725444852259 1101 68-69 0.0690460169078122 1101 70-71 0.005504743030222414 1101 72-73 0.04352752102563784 1101 74-75 -0.0029844193704491317 1101 76-77 -0.05950026381019313 1101 78-79 -0.12166881858620826 1101 80-81 -0.11488398793171939 1101 82-83 -0.08566429394726072 1101 84-85 -0.07841273348035216 1101 86-87 -0.15075047246384088 1101 88-89 -0.11388798731492855 1101 90-91 -0.16515503727849534 1101 92-93 -0.20784305214635168 1101 94-95 -0.1716363515393553 1101 96-97 -0.1509045474837194 1101 98-99 -0.11480583044295756 1101 100-101 0.12196822962855691 1102 1 0.31418446266870603 1102 2 0.32955785697881623 1102 3 0.34211068696301794 1102 4 0.18225541470938822 1102 5 0.2785405571419446 1102 6 0.23708423913365095 1102 7 0.25669605303178855 1102 8 0.2799023440426822 1102 9 0.26927613666263284 1102 10-11 0.29111489472044383 1102 12-13 0.2901133241112319 1102 14-15 0.45104954669625386 1102 16-17 0.4445857488589766 1102 18-19 0.45024288917934996 1102 20-21 0.4479095858076576 1102 22-23 0.5062934176994176 1102 24-25 0.5000420051858754 1102 26-27 0.44198164112961535 1102 28-29 0.47939060092486585 1102 30-31 0.41329208235797665 1102 32-33 0.3820928811146729 1102 34-35 0.3230702237457521 1102 36-37 0.3437831918148575 1102 38-39 0.2858299685702619 1102 40-41 0.19220782840681494 1102 42-43 0.14428591454440465 1102 44-45 0.12029938789061134 1102 46-47 0.08171164376071971 1102 48-49 0.14758734742852653 1102 50-51 0.014804221401057305 1102 52-53 0.016198010097717486 1102 54-55 0.07500810526013169 1102 56-57 0.10289491286048502 1102 58-59 0.06372228860172413 1102 60-61 0.005901800616648245 1102 62-63 0.07342500110870276 1102 64-65 0.12634464360162667 1102 66-67 0.10398525694929361 1102 68-69 -0.0027330868563097965 1102 70-71 0.0747151090028737 1102 72-73 0.06284893873512232 1102 74-75 0.07697220935082782 1102 76-77 0.009618403780507379 1102 78-79 0.021963732231434108 1102 80-81 0.029116428796484684 1102 82-83 0.03822484688649297 1102 84-85 0.05990408290116278 1102 86-87 0.05796704446349921 1102 88-89 0.04466572050552031 1102 90-91 0.02915481633412398 1102 92-93 0.05494010318543374 1102 94-95 0.013893483382545924 1102 96-97 0.06798881467602058 1102 98-99 0.03492488098411428 1102 100-101 0.3070411973070506 1103 1 0.30495456696291257 1103 2 0.24985260022069866 1103 3 0.24073755872862534 1103 4 0.2098209504218076 1103 5 0.24450637257873353 1103 6 0.2622798776890889 1103 7 0.2768134517324796 1103 8 0.23327127264979453 1103 9 0.34933677596142587 1103 10-11 0.2750403784400248 1103 12-13 0.26402613512009054 1103 14-15 0.4465734708294917 1103 16-17 0.405502965332154 1103 18-19 0.45162299217073354 1103 20-21 0.42293754532492756 1103 22-23 0.4650175665989664 1103 24-25 0.512536045393432 1103 26-27 0.4879137679605563 1103 28-29 0.44799449447727824 1103 30-31 0.44270945759613056 1103 32-33 0.3832746899568633 1103 34-35 0.2817242221938798 1103 36-37 0.3430315702158069 1103 38-39 0.3822408571383278 1103 40-41 0.24986610907635765 1103 42-43 0.22542766514486345 1103 44-45 0.20120485250543396 1103 46-47 0.17394196944717777 1103 48-49 0.23397277265528515 1103 50-51 0.08208289263524904 1103 52-53 0.0798226719728845 1103 54-55 0.13978025572654218 1103 56-57 0.155967516784699 1103 58-59 0.11613932334682886 1103 60-61 0.1356691791860456 1103 62-63 0.15222852126371578 1103 64-65 0.17314274645494265 1103 66-67 0.2406212931657663 1103 68-69 0.1719858986413172 1103 70-71 0.10691340152684603 1103 72-73 0.12729899174610892 1103 74-75 0.04278643749718469 1103 76-77 0.13418582778124488 1103 78-79 0.12387748402444032 1103 80-81 0.12105273090377011 1103 82-83 0.044311556117712314 1103 84-85 0.06214302760317025 1103 86-87 0.09068025716503314 1103 88-89 0.09061627374235215 1103 90-91 0.10122912262314188 1103 92-93 0.12055109623515392 1103 94-95 0.14624275122799446 1103 96-97 0.17793460084585533 1103 98-99 0.1904610614791764 1103 100-101 0.47145890201778684 1104 1 0.21294509230190073 1104 2 0.19631430596129462 1104 3 0.17278119605742148 1104 4 0.15339230143568017 1104 5 0.17592154952988892 1104 6 0.19251155144868193 1104 7 0.18632958449435932 1104 8 0.1656201169201097 1104 9 0.25651161482482365 1104 10-11 0.22767050422257995 1104 12-13 0.24950193673130627 1104 14-15 0.29768008791102574 1104 16-17 0.3098546714045298 1104 18-19 0.3272709945394041 1104 20-21 0.36313853753025427 1104 22-23 0.40266628680591765 1104 24-25 0.41101775207908275 1104 26-27 0.31071225899210475 1104 28-29 0.32342685631602563 1104 30-31 0.26734335001891196 1104 32-33 0.2288986412242906 1104 34-35 0.23792776755090728 1104 36-37 0.2641818356852781 1104 38-39 0.22014643828070035 1104 40-41 0.04620218055053016 1104 42-43 -0.001096899200696555 1104 44-45 0.07595123390678538 1104 46-47 0.09955658077394247 1104 48-49 0.08787260003227004 1104 50-51 -0.035384769453933984 1104 52-53 -0.0652822917682343 1104 54-55 0.0018967174473587534 1104 56-57 0.0666162304567095 1104 58-59 0.0039858763658315866 1104 60-61 -0.07542075619844724 1104 62-63 0.05388144695740493 1104 64-65 0.07459589142107603 1104 66-67 0.04470798219199423 1104 68-69 0.028621655030732995 1104 70-71 -0.03299472472421883 1104 72-73 -0.0050220413176873535 1104 74-75 0.0031560211610326405 1104 76-77 0.025885546175501872 1104 78-79 0.031986605999641426 1104 80-81 0.03238174464021881 1104 82-83 -7.422591324299788E-4 1104 84-85 -0.03621633742161379 1104 86-87 -0.02870549847034809 1104 88-89 -0.04227946979835906 1104 90-91 -0.05096791242325338 1104 92-93 -0.06728219839121152 1104 94-95 -0.05255402764865735 1104 96-97 -0.0239535325031639 1104 98-99 0.03069957933414713 1104 100-101 0.3529992985178758 1105 1 0.09662503176437554 1105 2 0.038121090232500876 1105 3 0.11229508751875983 1105 4 0.0800360374755229 1105 5 0.09218064219616195 1105 6 0.11942932325619182 1105 7 0.10865353917245812 1105 8 0.09755188149155458 1105 9 0.10924639958000171 1105 10-11 0.02538930979881826 1105 12-13 0.1356509553381784 1105 14-15 0.2365272836994663 1105 16-17 0.23802898358618307 1105 18-19 0.21499907699335807 1105 20-21 0.15341778144973972 1105 22-23 0.22103564904961814 1105 24-25 0.23589875897065582 1105 26-27 0.20021019246292582 1105 28-29 0.249413274388381 1105 30-31 0.22427119290482977 1105 32-33 0.13395286460013978 1105 34-35 0.07382902677910153 1105 36-37 0.14342679084874277 1105 38-39 0.08915666549588508 1105 40-41 -0.0038966808126517094 1105 42-43 -0.04219534797405089 1105 44-45 -0.08126064623218099 1105 46-47 -0.11441532351837225 1105 48-49 -0.03715152737534311 1105 50-51 -0.15686455437971603 1105 52-53 -0.1013314197293056 1105 54-55 0.011963300617011896 1105 56-57 0.02677770307282401 1105 58-59 6.7071830329723525E-6 1105 60-61 -0.07604512890229387 1105 62-63 -0.02427091145696636 1105 64-65 0.007665520171343587 1105 66-67 0.024753963087192687 1105 68-69 0.0099446560104397 1105 70-71 0.025345249171643047 1105 72-73 0.01996586209991591 1105 74-75 0.06781575566859033 1105 76-77 0.06517465582830795 1105 78-79 0.051476421380492354 1105 80-81 0.022618556908639675 1105 82-83 0.0050182777077765195 1105 84-85 -0.037008286835050086 1105 86-87 -1.302309317559036E-4 1105 88-89 -0.009202428751919456 1105 90-91 -0.05066983053114882 1105 92-93 -0.010930404896832613 1105 94-95 -0.030628613577484032 1105 96-97 0.08383993889687957 1105 98-99 0.11254859122647787 1105 100-101 0.4198320203459076 1106 1 0.043839882038668065 1106 2 0.03557639071207319 1106 3 0.016818066348626814 1106 4 0.03076916907323124 1106 5 0.006059053554487548 1106 6 0.0627662362797139 1106 7 0.05520957186033115 1106 8 0.07284165522111152 1106 9 0.0049125874507964795 1106 10-11 0.047251052328945775 1106 12-13 0.05597189794116275 1106 14-15 0.13138398501740056 1106 16-17 0.0926086495794678 1106 18-19 0.09797548091257369 1106 20-21 0.06118604939415917 1106 22-23 0.09600089475698326 1106 24-25 0.14139267848665327 1106 26-27 0.130976284164241 1106 28-29 0.1119754311172656 1106 30-31 0.08716409879171039 1106 32-33 -0.012339292622996823 1106 34-35 -0.04715027681317707 1106 36-37 -0.01363296536361247 1106 38-39 0.0034341167087248436 1106 40-41 -0.11282594249848188 1106 42-43 -0.20007947703429352 1106 44-45 -0.17755116373161428 1106 46-47 -0.1892398545292977 1106 48-49 -0.13482096414486477 1106 50-51 -0.2802330751774136 1106 52-53 -0.1955573397206507 1106 54-55 -0.10700292866911099 1106 56-57 -0.05916928809983801 1106 58-59 -0.05330960857287437 1106 60-61 -0.0505601968474636 1106 62-63 -0.04951008302052884 1106 64-65 0.006130398281655403 1106 66-67 0.05435506566058734 1106 68-69 0.01310778351233921 1106 70-71 0.02154697065694222 1106 72-73 0.047749213368128096 1106 74-75 0.021600480734718985 1106 76-77 0.018630352059886945 1106 78-79 0.02284098291847414 1106 80-81 0.030574641326182928 1106 82-83 0.037921884701685116 1106 84-85 1.561611476716962E-4 1106 86-87 -0.0364229001613019 1106 88-89 0.01953031792988469 1106 90-91 0.02405767925004909 1106 92-93 0.061457473091756754 1106 94-95 0.04443190571149458 1106 96-97 0.0321073732710353 1106 98-99 0.03939101239824083 1106 100-101 0.348242247935854 1107 1 -0.024389614308013563 1107 2 -0.02186228285604841 1107 3 -0.017214120250919507 1107 4 -0.049356070640421024 1107 5 -0.11334120102488043 1107 6 -0.0934364641211829 1107 7 -0.018257022875864948 1107 8 -0.0810105808135333 1107 9 -0.10984038051876865 1107 10-11 -0.06211427332480213 1107 12-13 -0.08587812438405251 1107 14-15 -0.0764832137732867 1107 16-17 -0.05112403826115752 1107 18-19 -0.017893889596074075 1107 20-21 -0.03196132059482437 1107 22-23 -0.07598915264258466 1107 24-25 0.02142680206271308 1107 26-27 -0.05180070073723897 1107 28-29 -0.0945145247818644 1107 30-31 -0.08341990983047509 1107 32-33 -0.19414887323603836 1107 34-35 -0.28287498536844424 1107 36-37 -0.30809252860589 1107 38-39 -0.5481509943287151 1107 40-41 -0.5558977503309208 1107 42-43 -0.47535091899042214 1107 44-45 -0.42736463789648127 1107 46-47 -0.6423351759287428 1107 48-49 -0.5598505801488898 1107 50-51 -0.546073135709527 1107 52-53 -0.41039545066815464 1107 54-55 -0.28102451922912763 1107 56-57 -0.2834324253692557 1107 58-59 -0.31134912126959335 1107 60-61 -0.3674048225522668 1107 62-63 -0.4070541772922951 1107 64-65 -0.2837726832815264 1107 66-67 -0.1648352669258344 1107 68-69 -0.11904616209611874 1107 70-71 -0.10812352624483168 1107 72-73 -0.11938176247887355 1107 74-75 -0.14186710172448613 1107 76-77 -0.10270202521670058 1107 78-79 -0.2341759954045486 1107 80-81 -0.19542153576285415 1107 82-83 -0.1734839694529242 1107 84-85 -0.1564843309826749 1107 86-87 -0.16608101685952192 1107 88-89 -0.20272558006064756 1107 90-91 -0.252688600698562 1107 92-93 -0.19798823080417804 1107 94-95 -0.20839022576426913 1107 96-97 -0.20211661591834584 1107 98-99 -0.18147529061383594 1107 100-101 0.12493329939095332 1108 1 -0.39831257755659166 1108 2 -0.25732776392938916 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-1.073135893701732 2308 40-41 -1.2850229568745348 2308 42-43 -1.2525310430773189 2308 44-45 -1.1937693662604687 2308 46-47 -1.2227142425728559 2308 48-49 -1.0804467988459194 2308 50-51 -1.5353566255897633 2308 52-53 -1.3879555594031459 2308 54-55 -0.9600649436060991 2308 56-57 -0.9869140217330639 2308 58-59 -1.192044749346202 2308 60-61 -1.1765749399589467 2308 62-63 -1.1346625736776303 2308 64-65 -1.3025737281465126 2308 66-67 -1.0184767307872846 2308 68-69 -0.9154546192508093 2308 70-71 -0.7879012507805072 2308 72-73 -0.7875318877792878 2308 74-75 -1.0334110810740782 2308 76-77 -0.9105748687662185 2308 78-79 -0.821767915175954 2308 80-81 -0.869371216042417 2308 82-83 -0.8300229019642273 2308 84-85 -0.8202403237756002 2308 86-87 -0.8011256269620013 2308 88-89 -0.8908727175535951 2308 90-91 -0.9528082876886081 2308 92-93 -0.9153861838736503 2308 94-95 -0.8892586587020546 2308 96-97 -0.9303281566463966 2308 98-99 -0.9498860309285888 2308 100-101 -0.5000548943731644 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 117.0 11 893.0 12 2799.0 13 5719.0 14 8571.0 15 11684.0 16 15736.0 17 24303.0 18 53806.0 19 161026.0 20 177074.0 21 108454.0 22 88942.0 23 85889.0 24 90173.0 25 101343.0 26 120873.0 27 142067.0 28 161604.0 29 182343.0 30 203769.0 31 238671.0 32 294713.0 33 392772.0 34 583058.0 35 1026779.0 36 1809946.0 37 2826236.0 38 2765121.0 39 454317.0 40 391.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 28.185329362183648 34.508504322390046 18.253618989419625 19.052547326006685 2 21.15748412450449 24.619467635345437 36.93590412874938 17.287144111400696 3 20.63580069231199 30.682142657658822 21.548667878572385 27.1333887714568 4 28.851489270940323 21.833111461496248 29.483278217562482 19.83212105000095 5 21.092782273595144 30.298138257883927 20.453079526191882 28.15599994232904 6 21.16450851418853 21.7253109951474 27.841225552162868 29.268954938501203 7 29.208014589595145 21.863262581573437 20.158707484977008 28.77001534385441 8 36.68827162273595 22.08061658150173 20.32563128182358 20.90548051393874 9 21.012958854750604 29.678471133576455 20.579742140950096 28.72882787072284 10-11 33.508207714023754 21.623922189207022 20.434567710036667 24.433302386732556 12-13 28.952912014722564 21.4878505073238 20.665913458805736 28.893324019147897 14-15 28.672691505775923 21.55916086658171 20.769058726323586 28.999088901318785 16-17 28.56921672698096 25.561730230764983 20.64731254721279 25.221740495041267 18-19 25.013752976928444 21.68584147706725 24.707661713837577 28.59274383216673 20-21 24.36089228358729 29.736086180379417 21.52852455559226 24.374496980441034 22-23 19.99228119833366 25.255124106303633 29.698138837929054 25.05445585743365 24-25 20.00238071897713 21.507477022766757 33.95286670692197 24.53727555133415 26-27 19.898395197702648 21.510838037793295 38.4545509214371 20.13621584306696 28-29 19.99806412124697 21.395970406592202 38.633870958441214 19.97209451371961 30-31 19.99266013630234 21.285996017856217 38.62875117697309 20.09259266886835 32-33 19.95812945845541 21.291854529215488 38.697822615685936 20.052193396643165 34-35 20.00605229888092 21.418910274813253 38.57967365035671 19.99536377594912 36-37 20.052977175577613 21.348047110227288 38.43837191773092 20.16060379646418 38-39 20.17366128881785 21.514467964080673 37.84396721433692 20.467903532764552 40-41 21.31517836033541 22.624314307626786 34.42307518046921 21.637432151568596 42-43 21.456742688910243 22.89980154697063 34.45281090442576 21.190644859693368 44-45 21.56297564527325 23.71573382099161 33.58830140764537 21.132989126089768 46-47 21.270916758037398 24.54116790329503 33.15755005070355 21.030365287964024 48-49 21.208840952320543 24.91395224887328 32.70589718094865 21.171309617857535 50-51 21.164164323148054 25.00205945158946 32.573867997720434 21.259908227542052 52-53 21.170036663890365 24.967403765474174 32.62135180121715 21.241207769418306 54-55 21.2554632731666 24.605880891696632 32.63904406317142 21.499611771965345 56-57 21.21262534076191 24.47595724826867 32.763900624899215 21.547516786070204 58-59 21.315377431379652 24.533461865154116 32.606706220791295 21.544454482674936 60-61 21.486423905258984 24.46935424563766 32.30129439697941 21.742927452123944 62-63 21.62894971420851 24.632285458658686 31.94079527311253 21.797969554020277 64-65 21.721737937732733 24.85985612127151 31.499304825730697 21.919101115265065 66-67 21.83156265966998 24.863259473017596 31.122573984303266 22.18260388300916 68-69 21.87818132840824 25.07153689826092 30.906843866847787 22.14343790648305 70-71 22.05056103413819 25.27673592719119 30.65327258243754 22.019430456233078 72-73 22.16175461459949 25.23203360356004 30.510544772756663 22.0956670090838 74-75 22.132992781394528 25.280869882015722 30.50259213653711 22.08354520005264 76-77 22.203942334905083 25.356658601356312 30.380484856917455 22.05891420682115 78-79 22.249099268816643 25.18562977204022 30.40331098851883 22.161959970624316 80-81 22.174657647095263 25.229060438245988 30.44078163028456 22.15550028437418 82-83 22.30982351386153 25.31174450802358 30.29181004375392 22.086621934360966 84-85 22.308866961026837 25.128149976888725 30.312860174031066 22.25012288805337 86-87 22.28055872498792 25.200322976610966 30.345673366815333 22.173444931585777 88-89 22.40789948071594 25.279031103620426 30.185553094098243 22.12751632156539 90-91 22.537996451828803 25.139173104185176 30.076136450088125 22.2466939938979 92-93 22.51173477342223 25.19044923004934 30.068601389657502 22.229214606870933 94-95 22.57281182497788 25.291602142499592 29.900712860842273 22.23487317168026 96-97 22.548885057404984 25.236716782585987 29.78941346168896 22.42498469832007 98-99 22.57884239626811 25.25917783248273 29.79915082379944 22.362828947449717 100-101 22.689572876528285 25.32379909275994 29.641136805211943 22.345491225499828 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 106051.0 1 59142.0 2 8496.0 3 3945.5 4 2743.5 5 2009.5 6 1562.0 7 1410.5 8 1310.5 9 1254.5 10 1344.0 11 1578.5 12 3071.5 13 6887.5 14 9726.5 15 20468.5 16 393182.0 17 412091.0 18 59354.5 19 44823.0 20 34121.0 21 25458.5 22 21341.0 23 21170.5 24 23325.0 25 27123.0 26 32700.0 27 40070.0 28 48927.0 29 58793.0 30 71005.0 31 85232.0 32 101081.5 33 119643.5 34 139093.5 35 159149.0 36 182641.0 37 205641.5 38 227410.5 39 247573.0 40 263553.5 41 277958.0 42 294265.5 43 312715.0 44 335706.5 45 363303.0 46 391844.0 47 420814.5 48 449098.0 49 469445.5 50 474010.5 51 477221.5 52 485778.5 53 491405.0 54 486762.0 55 468530.0 56 437391.0 57 397675.0 58 356737.5 59 316156.0 60 273408.5 61 230085.0 62 187226.0 63 143167.5 64 104077.5 65 73844.0 66 51920.0 67 38147.5 68 28262.5 69 20628.5 70 15597.0 71 11661.5 72 9126.0 73 6837.5 74 4993.5 75 3935.0 76 2955.0 77 1843.5 78 1052.5 79 578.0 80 255.0 81 107.0 82 61.5 83 32.0 84 17.0 85 9.5 86 7.5 87 5.0 88 6.0 89 5.5 90 2.5 91 2.0 92 1.5 93 2.5 94 4.5 95 3.0 96 1.0 97 1.5 98 1.5 99 2.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0025042857060479326 2 0.018502058209814657 3 0.006030056371141732 4 0.0065984633241591905 5 0.011244572331432329 6 0.002759656945809399 7 0.001054436086757024 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 8.773237753095552E-4 34-35 8.23778192778925E-6 36-37 0.0 38-39 3.501057319310432E-4 40-41 0.0 42-43 3.7481907771441095E-4 44-45 7.290437006093487E-4 46-47 0.0 48-49 0.0 50-51 2.96560149400413E-4 52-53 0.0011079816692876542 54-55 5.06623588559039E-4 56-57 2.6772791265315064E-4 58-59 3.542246228949378E-4 60-61 0.003060335986173707 62-63 0.007990648469955574 64-65 0.0012150728343489146 66-67 0.007183345841032227 68-69 0.0 70-71 0.0 72-73 0.0 74-75 5.972391897647207E-4 76-77 0.001363352909049121 78-79 0.004773794627153871 80-81 0.00209239660965847 82-83 0.001342758454229648 84-85 7.661137192844004E-4 86-87 0.0018988087343554225 88-89 0.002187131101828046 90-91 0.0021459421921890998 92-93 0.004839696882576185 94-95 3.707001867505163E-5 96-97 0.0 98-99 0.001079149432540392 100-101 5.39574716270196E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.213919E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.372034499844204 #Duplication Level Percentage of deduplicated Percentage of total 1 74.98722085424326 34.023227716461626 2 13.245623222272418 12.019617476257634 3 4.42084372551578 6.017480220975653 4 2.0156375968642974 3.6581431433643985 5 1.1217379988165095 2.544776759104443 6 0.7213785231700708 1.9638246744431467 7 0.5040365036587802 1.6008413143230915 8 0.3941357399713136 1.4306192313280053 9 0.28326181518232535 1.1566948365846863 >10 2.134978558738553 19.475375906084555 >50 0.15390250708842723 4.5266675758414525 >100 0.01508854289184895 1.103473355221428 >500 8.075319731546618E-4 0.24483581159021273 >1k 0.0010320268587267103 0.955887964416959 >5k 1.5742637736171938E-4 0.46664412216736234 >10k+ 1.5742637736171938E-4 8.811889891835314 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 833064 6.862599563891825 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 114027 0.939329559880025 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT 59544 0.4905104871082832 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 29897 0.24628496629511523 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25855 0.2129878517429911 No Hit GTCGACGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16619 0.13690369785792955 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.001342758454229648 0.0 0.0 0.0 0.0 2 0.0013592340180852264 0.0 0.0 0.0 0.0 3 0.0013839473638685943 0.0 0.0 0.0 1.64755638555785E-5 4 0.001408660709651962 0.0 0.0 0.0 2.4713345783367755E-5 5 0.0014333740554353298 0.0 0.0 0.0 4.118890963894626E-5 6 0.0014992763108576438 0.0 0.0 0.0 4.118890963894626E-5 7 0.0015487030024243793 0.0 0.0 0.0 4.118890963894626E-5 8 0.0015898919120633254 0.0 0.0 0.0 4.942669156673551E-5 9 0.0017134586409801643 0.0 0.0 0.0 7.414003735010327E-5 10-11 0.0017875986783302674 0.0 0.0 1.2356672891683876E-5 8.649671024178714E-5 12-13 0.0018658576066442655 0.0 0.0 2.0594454819473126E-5 1.31804510844628E-4 14-15 0.002187131101828046 0.0 0.0 2.4713345783367755E-5 1.606367475918904E-4 16-17 0.0025907824162897195 1.2356672891683876E-5 0.0 2.4713345783367755E-5 1.935878753030474E-4 18-19 0.0028008458554483456 1.64755638555785E-5 0.0 2.4713345783367755E-5 2.224201120503098E-4 20-21 0.0029532448211124465 2.0594454819473126E-5 0.0 2.4713345783367755E-5 2.5125234879757214E-4 22-23 0.003258042752440649 2.8832236747262376E-5 0.0 2.4713345783367755E-5 2.842034765087292E-4 24-25 0.003587554029552219 3.2951127711157E-5 0.0 2.4713345783367755E-5 3.1303571325599157E-4 26-27 0.0039376597614832615 3.2951127711157E-5 0.0 2.4713345783367755E-5 3.4186795000325395E-4 28-29 0.004246576583775359 3.2951127711157E-5 0.0 2.4713345783367755E-5 3.6246240482272707E-4 30-31 0.004658465680164822 3.2951127711157E-5 0.0 2.4713345783367755E-5 3.6246240482272707E-4 32-33 0.0050579981036626 4.118890963894625E-5 0.0 3.707001867505163E-5 3.830568596422002E-4 34-35 0.005498719436799325 5.766447349452476E-5 0.0 4.118890963894626E-5 3.9541353253388407E-4 36-37 0.005935321878972156 5.766447349452476E-5 0.0 4.5307800602840884E-5 4.2424576928114645E-4 38-39 0.006466658813314562 7.825892831399789E-5 0.0 5.766447349452476E-5 4.448402241006196E-4 40-41 0.007459311535613166 9.061560120568177E-5 0.0 5.766447349452476E-5 4.5719689699230345E-4 42-43 0.008085382962125149 9.061560120568177E-5 0.0 6.5902255422314E-5 4.983858066312497E-4 44-45 0.00897506341032639 9.885338313347102E-5 0.0 7.414003735010325E-5 5.107424795229335E-4 46-47 0.010494934176003506 1.0297227409736564E-4 0.0 8.237781927789252E-5 5.395747162701958E-4 48-49 0.012055993851319568 1.3592340180852263E-4 0.0 8.237781927789252E-5 5.848825168730369E-4 50-51 0.013460535670007635 1.4416118373631188E-4 0.0 8.237781927789252E-5 6.095958626564046E-4 52-53 0.01459323068507866 1.5651785662799578E-4 0.0 8.237781927789252E-5 6.301903174758777E-4 54-55 0.01584125464713873 1.5651785662799578E-4 0.0 8.237781927789252E-5 6.425469903675616E-4 56-57 0.017048089699559857 1.6887452951967966E-4 0.0 8.237781927789252E-5 6.672603361509294E-4 58-59 0.018489701536922976 1.7299342048357429E-4 0.0 9.061560120568177E-5 6.837359000065079E-4 60-61 0.01999721562970841 1.7299342048357429E-4 0.0 9.061560120568177E-5 7.331625915732434E-4 62-63 0.022204941186355926 1.771123114474689E-4 0.0 9.061560120568177E-5 7.455192644649272E-4 64-65 0.024750415802042805 1.8946898433915279E-4 0.0 9.061560120568177E-5 7.661137192844004E-4 66-67 0.02822675977556987 1.8946898433915279E-4 0.0 9.061560120568177E-5 7.949459560316627E-4 68-69 0.03267928090753996 1.8946898433915279E-4 0.0 9.061560120568177E-5 8.237781927789251E-4 70-71 0.03927362534073525 1.8946898433915279E-4 0.0 9.061560120568177E-5 8.526104295261876E-4 72-73 0.04776677850828598 1.935878753030474E-4 0.0 9.061560120568177E-5 8.649671024178714E-4 74-75 0.058039292572239165 2.059445481947313E-4 0.0 9.061560120568177E-5 8.979182301290284E-4 76-77 0.0702064964795839 2.100634391586259E-4 0.0 9.473449216957639E-5 9.143937939846069E-4 78-79 0.08577178543214167 2.3065789397809904E-4 0.0 9.885338313347102E-5 9.143937939846069E-4 80-81 0.10541065754799125 2.3477678494199366E-4 0.0 9.885338313347102E-5 9.391071397679747E-4 82-83 0.12958030972412493 2.5125234879757214E-4 0.0 9.885338313347102E-5 9.679393765152371E-4 84-85 0.16069441206538493 2.759656945809399E-4 0.0 9.885338313347102E-5 9.926527222986047E-4 86-87 0.1993543226524999 2.9656014940041307E-4 0.0 9.885338313347102E-5 0.0010256038500097617 88-89 0.24581953161619516 3.006790403643077E-4 0.0 9.885338313347102E-5 0.0010585549777209187 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 95355 0.0 94.34838 1 TCGACGG 97340 0.0 92.43903 2 ATATCTC 108620 0.0 92.252266 1 CGACGGC 99440 0.0 90.359245 3 TATCTCG 111085 0.0 90.072586 2 ACGGCGC 99965 0.0 89.845505 5 GACGGCG 100395 0.0 89.55096 4 CGGCGCG 100885 0.0 88.9964 6 ATCTCGA 115990 0.0 86.19023 3 TCTCGAG 118115 0.0 83.79381 4 CTCGAGG 121120 0.0 81.73807 5 TCGAGGG 122110 0.0 81.112495 6 CGAGGGC 124260 0.0 79.62998 7 AGGGCGC 125505 0.0 79.00181 9 GAGGGCG 127165 0.0 78.01535 8 CTCGACG 2365 0.0 41.37688 5 CGCGCCG 224145 0.0 40.83846 9 CCATATT 108490 0.0 40.79697 18-19 TCCATAT 108840 0.0 40.563225 18-19 ATCCATA 109230 0.0 40.50971 16-17 >>END_MODULE