FastQCFastQC Report
Tue 31 May 2016
SRR1051268_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051268_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12139190
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6780195.585372664897741No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT583280.4804933442840914No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT529980.43658596660897475No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT393380.3240578654753736No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167590.13805698732782004No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159640.1315079506952276No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT137550.11331069041674116No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126000.10379605229014456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG63700.086.779091
TCGACGG70100.079.2825852
TATCTCG107000.065.126292
ATATCTC110750.063.1740761
CGACGGC93850.059.1677253
ACGGCGC101750.054.4324725
GACGGCG105100.052.6983344
CGGCGCG112600.049.2287946
ATCTCGA168800.041.2540053
TCTCGAG199000.034.945324
TCGAGGG229300.030.3680326
CTCGAGG230550.030.32755
CGAGGGC253050.027.5929247
AGGGCGC272950.025.6160079
GAGGGCG294500.023.7738178
CGCGCCG386750.020.3752449
GGGCGCG271900.020.2295448
GGCGCGC406700.019.4108077
AATTCCC62300.019.3785151
ATTCCCC60000.019.2382262
Produced by FastQC (version 0.11.5)