##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051268_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12139190 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.56540872990702 34.0 31.0 34.0 31.0 34.0 2 32.81440837485862 34.0 31.0 34.0 31.0 34.0 3 32.902767318083 34.0 31.0 34.0 31.0 34.0 4 36.342884327537504 37.0 37.0 37.0 35.0 37.0 5 36.2330034376264 37.0 37.0 37.0 35.0 37.0 6 36.204659783725276 37.0 37.0 37.0 35.0 37.0 7 36.18812408406162 37.0 37.0 37.0 35.0 37.0 8 36.17451584496165 37.0 37.0 37.0 35.0 37.0 9 37.906000235600565 39.0 38.0 39.0 35.0 39.0 10-11 37.886206040106465 39.0 38.0 39.0 35.0 39.0 12-13 37.86553678622708 39.0 38.0 39.0 35.0 39.0 14-15 39.34456116923782 41.0 39.0 41.0 36.0 41.0 16-17 39.26272729070062 40.5 39.0 41.0 36.0 41.0 18-19 39.06991376689878 40.0 38.0 41.0 35.5 41.0 20-21 39.022639072293956 40.0 38.0 41.0 35.0 41.0 22-23 38.98131905835562 40.0 38.0 41.0 35.0 41.0 24-25 38.93597183996626 40.0 38.0 41.0 35.0 41.0 26-27 38.82939528914203 40.0 38.0 41.0 35.0 41.0 28-29 38.68821593533012 40.0 38.0 41.0 35.0 41.0 30-31 38.55559814946467 40.0 38.0 41.0 35.0 41.0 32-33 38.47510678224824 40.0 37.5 41.0 35.0 41.0 34-35 38.36683213624632 40.0 37.0 41.0 35.0 41.0 36-37 38.28136634322389 40.0 37.0 41.0 34.0 41.0 38-39 38.18568660676701 40.0 37.0 41.0 34.0 41.0 40-41 38.20450194782353 40.0 37.0 41.0 34.0 41.0 42-43 38.201283858313445 40.0 37.0 41.0 34.0 41.0 44-45 38.14111719974727 40.0 37.0 41.0 34.0 41.0 46-47 38.039523477266606 40.0 36.5 41.0 34.0 41.0 48-49 37.96555132591219 40.0 36.0 41.0 33.0 41.0 50-51 37.91300510989613 40.0 36.0 41.0 33.0 41.0 52-53 37.73918539869629 40.0 35.0 41.0 33.0 41.0 54-55 37.54594355142312 39.0 35.0 41.0 33.0 41.0 56-57 37.32733011840164 39.0 35.0 41.0 33.0 41.0 58-59 37.07808276334747 39.0 35.0 41.0 33.0 41.0 60-61 36.7659859512867 38.0 35.0 40.5 33.0 41.0 62-63 36.426638474230984 37.0 35.0 40.0 32.0 41.0 64-65 36.09127338809262 37.0 35.0 40.0 32.0 41.0 66-67 35.70439716323742 36.0 35.0 39.0 31.5 41.0 68-69 35.31415077941774 36.0 35.0 39.0 31.0 41.0 70-71 34.98273134368932 35.0 35.0 38.5 31.0 40.0 72-73 34.604868735063874 35.0 34.0 37.0 31.0 39.5 74-75 34.25587119074666 35.0 34.0 37.0 30.0 39.0 76-77 33.602273009978425 35.0 33.5 36.0 29.5 38.5 78-79 33.65077793493635 35.0 34.0 36.0 30.0 37.0 80-81 33.46569005015985 35.0 34.0 35.0 30.0 37.0 82-83 33.24749604380523 35.0 34.0 35.0 30.0 36.5 84-85 33.03155811878717 35.0 34.0 35.0 29.5 36.0 86-87 32.84840191149492 35.0 34.0 35.0 29.0 36.0 88-89 32.66201048010617 35.0 34.0 35.0 29.0 36.0 90-91 32.50446541326069 35.0 33.0 35.0 29.0 35.0 92-93 32.33852785894281 35.0 33.0 35.0 29.0 35.0 94-95 32.16906836452844 35.0 33.0 35.0 29.0 35.0 96-97 31.981427385187974 35.0 33.0 35.0 27.0 35.0 98-99 31.759970311033932 35.0 33.0 35.0 27.0 35.0 100-101 31.05387134561696 34.5 32.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.40054410264929174 1101 2 0.38687682256426115 1101 3 0.34855677138553887 1101 4 0.22901130787445823 1101 5 0.2433845203704479 1101 6 0.2857132067894739 1101 7 0.31572920735375476 1101 8 0.2845318396858616 1101 9 0.3868321044117309 1101 10-11 0.37051266460328947 1101 12-13 0.360008197281779 1101 14-15 0.47879998988845784 1101 16-17 0.48383784436053645 1101 18-19 0.45388569393654876 1101 20-21 0.4322042108763853 1101 22-23 0.4565964869468502 1101 24-25 0.4429739306431273 1101 26-27 0.40978405904409954 1101 28-29 0.45657066896158227 1101 30-31 0.4779939607168018 1101 32-33 0.4656297481881069 1101 34-35 0.446277314520259 1101 36-37 0.35069917099674086 1101 38-39 0.3017596498457209 1101 40-41 0.47215431076065073 1101 42-43 0.45101910467198536 1101 44-45 0.4242542888681129 1101 46-47 0.43857633808674024 1101 48-49 0.42138278331331946 1101 50-51 0.4215038032577212 1101 52-53 0.3844870986604363 1101 54-55 0.42956526611247625 1101 56-57 0.3927893783257801 1101 58-59 0.31152671519594577 1101 60-61 0.3626787603411472 1101 62-63 0.2052060394006645 1101 64-65 0.22742389277816244 1101 66-67 0.2826784093490673 1101 68-69 0.23501754736342662 1101 70-71 0.17187556146777894 1101 72-73 0.19719572763814597 1101 74-75 0.20279907765434046 1101 76-77 0.005960412577096008 1101 78-79 0.12169365870769866 1101 80-81 0.11959326073797172 1101 82-83 0.0897747092636223 1101 84-85 0.03971125363377581 1101 86-87 -0.03158261941082685 1101 88-89 -0.07109084646018005 1101 90-91 -0.060103790538178714 1101 92-93 -0.1201239448618594 1101 94-95 -0.18456519458958098 1101 96-97 -0.22792848470583138 1101 98-99 -0.24572538730235038 1101 100-101 -0.054247472482483516 1102 1 0.3532685164319602 1102 2 0.3141250830783022 1102 3 0.29571344078166106 1102 4 0.19421862526244382 1102 5 0.18013594860019566 1102 6 0.2441893333167613 1102 7 0.197839041378721 1102 8 0.24881143912769943 1102 9 0.3580138296952171 1102 10-11 0.2993763210651821 1102 12-13 0.3141057514967258 1102 14-15 0.39692576440821625 1102 16-17 0.30870803931395585 1102 18-19 0.35091186760978843 1102 20-21 0.3079267464675368 1102 22-23 0.29030814439019537 1102 24-25 0.2937443759965035 1102 26-27 0.3149300781576585 1102 28-29 0.3391003363342975 1102 30-31 0.30924830904869083 1102 32-33 0.2837118258779441 1102 34-35 0.249481048126043 1102 36-37 0.23665939856763885 1102 38-39 0.208226911472579 1102 40-41 0.3105106394907651 1102 42-43 0.3142015849644295 1102 44-45 0.26637368082312207 1102 46-47 0.2725680998492166 1102 48-49 0.28978978113793374 1102 50-51 0.3193969372483849 1102 52-53 0.30093083887796723 1102 54-55 0.29431943985839126 1102 56-57 0.2952424833878169 1102 58-59 0.23187976564292256 1102 60-61 0.24523536087098563 1102 62-63 0.2761137414061352 1102 64-65 0.2094660618964923 1102 66-67 0.194337830355785 1102 68-69 0.21609792688816043 1102 70-71 0.2702613172049624 1102 72-73 0.2865630996140922 1102 74-75 0.22556285048507618 1102 76-77 0.02866065477493862 1102 78-79 0.18429003122378163 1102 80-81 0.14008269638764403 1102 82-83 0.16593471162538265 1102 84-85 0.19424509280975855 1102 86-87 0.18325224643945148 1102 88-89 0.12454836656051782 1102 90-91 0.11015386933745219 1102 92-93 0.20843975262246772 1102 94-95 0.1500418136359869 1102 96-97 0.06666916734698702 1102 98-99 0.09901433525541137 1102 100-101 0.35222982293944227 1103 1 0.360881376039508 1103 2 0.29949449853970833 1103 3 0.305994450810104 1103 4 0.18116071877368967 1103 5 0.23207022064141825 1103 6 0.2215806411698864 1103 7 0.2416492873944449 1103 8 0.2615172523233298 1103 9 0.3110751280578725 1103 10-11 0.3358852934888148 1103 12-13 0.33028016640258784 1103 14-15 0.4513322757707243 1103 16-17 0.40154455780305653 1103 18-19 0.3699152762289586 1103 20-21 0.3453004246385518 1103 22-23 0.34557974726193663 1103 24-25 0.2951754292270863 1103 26-27 0.36127077929594975 1103 28-29 0.2867179973302285 1103 30-31 0.29923775916950035 1103 32-33 0.300281079458145 1103 34-35 0.2951673533491004 1103 36-37 0.19131630041929526 1103 38-39 0.20440011161662852 1103 40-41 0.23713920096635377 1103 42-43 0.23091079043570772 1103 44-45 0.2616720769330527 1103 46-47 0.21918656687286386 1103 48-49 0.293989890047861 1103 50-51 0.3328881444665228 1103 52-53 0.35515545667676207 1103 54-55 0.3433755849082871 1103 56-57 0.3845116770877368 1103 58-59 0.3566193820955661 1103 60-61 0.32847378309165975 1103 62-63 0.3151815760903105 1103 64-65 0.27664474890720214 1103 66-67 0.2446148363587639 1103 68-69 0.2986305418662596 1103 70-71 0.301237269641625 1103 72-73 0.24320988895375706 1103 74-75 0.24478974989406055 1103 76-77 0.10963032477160084 1103 78-79 0.27109042664383765 1103 80-81 0.29275095317838407 1103 82-83 0.27916396805301247 1103 84-85 0.2558959995751877 1103 86-87 0.18244073131742766 1103 88-89 0.22605174762851732 1103 90-91 0.21378573596585682 1103 92-93 0.22425726933159496 1103 94-95 0.19588418463920476 1103 96-97 0.1766068936048768 1103 98-99 0.20129276667812235 1103 100-101 0.434885054264214 1104 1 0.273208388575533 1104 2 0.24942698755849335 1104 3 0.23097002017952661 1104 4 0.14756241866300002 1104 5 0.1791222578845506 1104 6 0.20492578766821623 1104 7 0.23498864795536178 1104 8 0.19474089916297999 1104 9 0.27142602430008367 1104 10-11 0.29104749578448974 1104 12-13 0.2655975836325126 1104 14-15 0.32137704263917044 1104 16-17 0.3462744535556439 1104 18-19 0.2563165639142113 1104 20-21 0.2392383859175098 1104 22-23 0.2635214817160616 1104 24-25 0.23207368670984607 1104 26-27 0.25243693221561614 1104 28-29 0.25374065498068177 1104 30-31 0.2196630230554888 1104 32-33 0.2058798284691079 1104 34-35 0.21006384237591647 1104 36-37 0.166313409964026 1104 38-39 0.15690531946298591 1104 40-41 0.15887459511888835 1104 42-43 0.1697503279427508 1104 44-45 0.21105387138800324 1104 46-47 0.20756075364955962 1104 48-49 0.2362265042238576 1104 50-51 0.21652949979345237 1104 52-53 0.22022646636556686 1104 54-55 0.23880443044537003 1104 56-57 0.24413706905860266 1104 58-59 0.254793858521019 1104 60-61 0.2552198945496187 1104 62-63 0.2559002739678675 1104 64-65 0.2594546207921766 1104 66-67 0.24396027391336617 1104 68-69 0.28492706939898227 1104 70-71 0.2342787800980375 1104 72-73 0.24448723140383777 1104 74-75 0.25922795095512896 1104 76-77 0.04817940922617936 1104 78-79 0.22379335419746837 1104 80-81 0.2195895089360249 1104 82-83 0.16357324194049028 1104 84-85 0.274215654771524 1104 86-87 0.27054865689549956 1104 88-89 0.24277545680146773 1104 90-91 0.23569097578315734 1104 92-93 0.2722435645521415 1104 94-95 0.22226902689072858 1104 96-97 0.21916580611004122 1104 98-99 0.21325658722659568 1104 100-101 0.5149512683107176 1105 1 0.14280608318703258 1105 2 0.1602342651547204 1105 3 0.13453440125060467 1105 4 0.07716875523004774 1105 5 0.08668298994323465 1105 6 0.10439700125434825 1105 7 0.10988264006464732 1105 8 0.1173521579476855 1105 9 0.16004790126297763 1105 10-11 0.18345795842501644 1105 12-13 0.1810182082424916 1105 14-15 0.27846537415796746 1105 16-17 0.22376892867337972 1105 18-19 0.077881540014765 1105 20-21 0.05203444942930702 1105 22-23 0.027022034843596998 1105 24-25 0.05259010693684729 1105 26-27 0.08872883388202979 1105 28-29 0.06222761628046669 1105 30-31 0.12188676305099477 1105 32-33 0.08164629331966466 1105 34-35 0.04913100417228122 1105 36-37 0.03229127193048953 1105 38-39 0.029891015192646364 1105 40-41 0.021740028809084322 1105 42-43 -0.007429072529980374 1105 44-45 -0.03723710299248495 1105 46-47 0.013397078386887529 1105 48-49 0.09429290356564479 1105 50-51 0.06034026537003001 1105 52-53 0.08731919028175383 1105 54-55 0.05078176492960296 1105 56-57 0.035616325917644076 1105 58-59 0.010806608875974177 1105 60-61 0.033836384537686115 1105 62-63 0.06183220782752841 1105 64-65 0.1430256800534977 1105 66-67 0.15139329364336618 1105 68-69 0.16254630051189167 1105 70-71 0.1568282517865427 1105 72-73 0.1981972418102771 1105 74-75 0.16750271290133156 1105 76-77 -0.012963409939288795 1105 78-79 0.13527559277958545 1105 80-81 0.11297734620919186 1105 82-83 0.15732906464801033 1105 84-85 0.1660727250243852 1105 86-87 0.12612462792638723 1105 88-89 0.14950282703203754 1105 90-91 0.06956046672827654 1105 92-93 0.08287166020868142 1105 94-95 0.0474835679307688 1105 96-97 0.03827594041032967 1105 98-99 0.05585723426269951 1105 100-101 0.32499629042960976 1106 1 0.03686255924219495 1106 2 0.043179131697208106 1106 3 0.03803441011980624 1106 4 0.04292968330134528 1106 5 0.06030416796681948 1106 6 0.08683552891184831 1106 7 0.11248404396789624 1106 8 0.03040346237266789 1106 9 0.11752697725921735 1106 10-11 0.09444830809444937 1106 12-13 0.09012180368986122 1106 14-15 0.1545263931378429 1106 16-17 0.10610403271698488 1106 18-19 0.02802874626438978 1106 20-21 -0.03417925816702194 1106 22-23 -0.025161030781731597 1106 24-25 0.003807535452118316 1106 26-27 -0.0026384396876437677 1106 28-29 -0.017351801405808942 1106 30-31 -0.01327923062616776 1106 32-33 -0.05288221219475986 1106 34-35 -0.07681849977205246 1106 36-37 -0.09813935767036241 1106 38-39 -0.14377892138903547 1106 40-41 -0.08715281990290435 1106 42-43 -0.049699430778133546 1106 44-45 -0.11839045574918572 1106 46-47 -0.06705791925593019 1106 48-49 -0.011835066053158982 1106 50-51 -0.037265721537906416 1106 52-53 -0.006521670716715278 1106 54-55 0.010494287209340314 1106 56-57 0.015020271744937475 1106 58-59 -0.02453131884087867 1106 60-61 -0.02872924210296901 1106 62-63 -0.05306594723828084 1106 64-65 -0.00922090301753542 1106 66-67 0.01764668725823526 1106 68-69 0.04434471376477234 1106 70-71 0.056090533279451904 1106 72-73 0.06536534950625139 1106 74-75 0.05715232096419953 1106 76-77 -0.15943005953648992 1106 78-79 0.07510502743181036 1106 80-81 0.038129788086976646 1106 82-83 0.0614083948605213 1106 84-85 0.10721281492813262 1106 86-87 0.1492526554298763 1106 88-89 0.06567388708274535 1106 90-91 0.01962712982742687 1106 92-93 0.08530588877143686 1106 94-95 0.10162994136277348 1106 96-97 0.040687491908805384 1106 98-99 0.010257060651021277 1106 100-101 0.3818584248102894 1107 1 -0.047075179397104705 1107 2 -0.00982466764904899 1107 3 -0.009424546738152628 1107 4 0.006432557627675806 1107 5 -0.02382528476886847 1107 6 -0.0226109088285682 1107 7 0.014305374646035318 1107 8 0.01857361406712954 1107 9 -0.038574566845468894 1107 10-11 0.017397467591393934 1107 12-13 -0.007117070410913584 1107 14-15 0.0234911837501528 1107 16-17 -0.009876870610384003 1107 18-19 -0.12934758811022817 1107 20-21 -0.199091718851669 1107 22-23 -0.19031800862552473 1107 24-25 -0.20726693590282963 1107 26-27 -0.17938284114828207 1107 28-29 -0.22973375751158187 1107 30-31 -0.2797753886728316 1107 32-33 -0.2552878204086255 1107 34-35 -0.3699859047956693 1107 36-37 -0.3568319845574095 1107 38-39 -0.4639039133210403 1107 40-41 -0.5151433074967073 1107 42-43 -0.330843659652281 1107 44-45 -0.3262948180877885 1107 46-47 -0.26525121204581126 1107 48-49 -0.28705792318346823 1107 50-51 -0.16993861017780887 1107 52-53 -0.1370898692595901 1107 54-55 -0.1648232706350612 1107 56-57 -0.2110343176779068 1107 58-59 -0.19049618931629908 1107 60-61 -0.15796276487778727 1107 62-63 -0.16651050852695448 1107 64-65 -0.14224935625318835 1107 66-67 -0.0579042218849537 1107 68-69 -0.08468418419336388 1107 70-71 -0.06409807064225959 1107 72-73 -0.048039665718647484 1107 74-75 -0.13039835411235146 1107 76-77 -0.3765656024148356 1107 78-79 -0.1634521121709014 1107 80-81 -0.20537820542244845 1107 82-83 -0.25011200488550855 1107 84-85 -0.15678992928027924 1107 86-87 -0.018993864658582993 1107 88-89 -0.09755738087905286 1107 90-91 -0.15653216347254784 1107 92-93 -0.0946354597848682 1107 94-95 -0.12648206445490473 1107 96-97 -0.1790617803791008 1107 98-99 -0.2182905770936756 1107 100-101 0.10854143164878138 1108 1 -0.15198369452729565 1108 2 -0.14685380831348738 1108 3 -0.18336147042337103 1108 4 -0.10503521024733686 1108 5 -0.17283013021931737 1108 6 -0.08448976008467213 1108 7 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-0.6879507699311205 2308 52-53 -0.699141596236764 2308 54-55 -0.6480872470638133 2308 56-57 -0.7938820231163177 2308 58-59 -0.7166311703750807 2308 60-61 -0.8482724088168467 2308 62-63 -1.0058764935705682 2308 64-65 -0.8067353121605834 2308 66-67 -0.9414668072799941 2308 68-69 -0.9510377541428809 2308 70-71 -1.3168120345747454 2308 72-73 -1.0797091510331427 2308 74-75 -0.997908658583377 2308 76-77 -1.2758648666490515 2308 78-79 -1.1620150050230365 2308 80-81 -1.1511094082644746 2308 82-83 -1.2454722997601522 2308 84-85 -1.2629986525833345 2308 86-87 -1.354167959034399 2308 88-89 -1.3414583245510556 2308 90-91 -1.3070933265291558 2308 92-93 -1.2212438155835343 2308 94-95 -1.27342659605101 2308 96-97 -1.323949530298627 2308 98-99 -1.5425533439000496 2308 100-101 -1.5174416449345856 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 4.0 11 59.0 12 246.0 13 887.0 14 2532.0 15 5218.0 16 8675.0 17 13447.0 18 22890.0 19 40459.0 20 37872.0 21 34325.0 22 35689.0 23 40286.0 24 47038.0 25 58046.0 26 71883.0 27 92311.0 28 116333.0 29 142133.0 30 177076.0 31 226964.0 32 291852.0 33 392609.0 34 581513.0 35 1290701.0 36 1477027.0 37 3135119.0 38 3274032.0 39 521630.0 40 334.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 24.021042741089392 33.75602261329668 21.024869136167222 21.198065509446703 2 23.747759052390354 31.54370294751442 25.005408260658886 19.703129739436346 3 22.90025820771684 29.615825404660523 25.10623553842372 22.377680849198917 4 24.20012762303983 29.26535031173402 24.93342463230115 21.601097432925 5 23.83423048362417 30.478697822504124 23.169313357508145 22.51775833636356 6 23.91199083299627 29.248780190441042 22.777071616804744 24.06215735975794 7 24.19231431421701 29.491382868214437 23.24197907768146 23.07432373988709 8 23.88467434812372 29.151113047905174 23.413876873168636 23.550335730802466 9 23.34214226814145 28.964922700773283 23.733535763094572 23.959399267990698 10-11 24.03649666905288 29.195259321256195 23.44681152531594 23.321432484374988 12-13 23.622683888972894 29.04259715411573 23.733922873452855 23.600796083458526 14-15 23.2611854662461 29.215161802393734 23.826931615700882 23.696721115659283 16-17 23.436306090463415 29.592215129567638 23.694575155152762 23.276903624816185 18-19 23.0645578494117 29.39596464014485 24.31155620762176 23.227921302821688 20-21 22.66128959180967 29.56555585669225 24.559525800321108 23.213628751176973 22-23 22.378824287287703 29.315028432704327 25.239385823930593 23.066761456077383 24-25 22.430298067663493 28.905828972114282 25.86247929227568 22.80139366794654 26-27 22.333075765351722 28.937177851240488 26.234468691897895 22.495277691509894 28-29 22.46632600692468 29.083262557056937 26.12949051790111 22.32092091811727 30-31 22.454299780915736 28.959730755780978 26.135376691889434 22.45059277141385 32-33 22.38905149355105 29.006614115109823 26.202328985706625 22.4020054056325 34-35 22.46404002243972 29.147817935134057 26.045308624381036 22.342833418045192 36-37 22.51224340339018 29.098139991218524 25.89421534715249 22.49540125823881 38-39 22.419395363282064 29.185827060948878 25.91592190253221 22.478855673236847 40-41 22.573149665968344 29.444739770653864 25.56798808875669 22.4141224746211 42-43 22.591969464820043 29.38510213026359 25.456726746377445 22.56620165853892 44-45 22.508648730359383 29.548766729562782 25.45082755549954 22.491756984578302 46-47 22.57152896673908 29.712952437259744 25.253741909805832 22.461776686195346 48-49 22.60622413851336 29.61478484149272 25.21201167458455 22.566979345409372 50-51 22.530992595057825 29.610719496111354 25.327855482944084 22.530432425886733 52-53 22.557250524952654 29.731921981614917 25.243739491679428 22.467088001752998 54-55 22.623786846948974 29.60570618279228 25.197777923037414 22.57272904722134 56-57 22.511141502133032 29.684498163776986 25.290780447401552 22.513579886688433 58-59 22.57511441669747 29.78213686738834 25.17540104229437 22.467347673619827 60-61 22.6237994462563 29.632500191528433 25.11803917724329 22.625661184971978 62-63 22.591494984426475 29.706541375495398 25.160620272028034 22.541343368050093 64-65 22.6122541948845 29.820968285363357 25.083675269931522 22.48310224982062 66-67 22.611207996579672 29.68753681258799 25.042992983881128 22.658262206951203 68-69 22.593024176818684 29.744703704812753 25.083495209453993 22.57877690891457 70-71 22.67084316752956 29.831436844751323 25.00459462324872 22.493125364470394 72-73 22.68385430053316 29.714094123384537 24.98375890521099 22.61829267087131 74-75 22.590560481769483 29.75772017714517 25.066933595110374 22.584785745974976 76-77 22.67531082723412 29.821221257949183 24.956518744863207 22.546949169953486 78-79 22.702318440913196 29.675191896338987 24.96508391968898 22.65740574305884 80-81 22.551794373295138 29.726363092473733 25.057975387156468 22.66386714707466 82-83 22.642942112891152 29.818215907723715 24.936866479198898 22.601975500186235 84-85 22.673888896028302 29.660715362510242 24.91436000496903 22.751035736492426 86-87 22.587554009523792 29.732551506677325 25.000625042295905 22.67926944150298 88-89 22.678380134592818 29.82317434228411 24.86549697293019 22.63294855019288 90-91 22.732495687813913 29.64040835002829 24.83955932808939 22.787536634068406 92-93 22.681661125553187 29.662800964799178 24.89670059324383 22.758837316403806 94-95 22.753555260386552 29.739463308940085 24.82438865396056 22.682592776712802 96-97 22.688940779660953 29.66545746852298 24.793721549752888 22.85188020206317 98-99 22.71114637280143 29.656821576002596 24.807038491140883 22.82499356005509 100-101 22.75735165097149 29.680239038323997 24.703199792667814 22.859209518036696 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 596195.0 1 333346.0 2 47397.0 3 21033.0 4 15620.5 5 11814.0 6 9392.0 7 8379.5 8 7901.5 9 7645.5 10 7725.5 11 8435.5 12 12244.0 13 21293.5 14 28364.5 15 28922.5 16 82589.5 17 78004.5 18 15297.5 19 10552.5 20 9028.5 21 8812.0 22 9943.5 23 12149.0 24 14934.0 25 18173.5 26 22588.0 27 28379.5 28 34856.5 29 42359.5 30 51696.5 31 61356.0 32 72298.5 33 86614.5 34 102194.0 35 119367.5 36 142941.0 37 167100.0 38 190361.5 39 214536.0 40 237597.5 41 259101.0 42 280657.5 43 304662.5 44 331619.5 45 361803.5 46 395741.0 47 433671.5 48 468244.0 49 494584.5 50 506825.0 51 512869.0 52 523087.0 53 531533.5 54 528464.5 55 509850.0 56 475841.0 57 433370.5 58 389871.5 59 347268.5 60 302534.0 61 254507.5 62 207060.0 63 159779.5 64 117062.0 65 82618.5 66 58116.5 67 43371.0 68 32068.0 69 23319.0 70 17999.0 71 13411.5 72 10133.5 73 7687.0 74 5792.0 75 4546.5 76 3464.0 77 2260.0 78 1305.5 79 690.5 80 357.5 81 229.5 82 163.0 83 118.0 84 85.5 85 52.5 86 25.5 87 8.5 88 4.0 89 2.5 90 1.5 91 2.0 92 2.5 93 2.0 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06961749507174696 2 0.002899699238581816 3 0.0010379605229014455 4 0.002603139089181403 5 0.003163308260271072 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 8.649671024178714E-5 14-15 0.0 16-17 8.690859933817661E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 2.0594454819473126E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 5.848825168730368E-4 42-43 9.473449216957639E-5 44-45 0.0010297227409736564 46-47 2.842034765087292E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 5.3545582530630134E-5 56-57 4.530780060284088E-5 58-59 7.414003735010325E-5 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.6887452951967966E-4 70-71 8.23778192778925E-6 72-73 0.0018946898433915278 74-75 8.773237753095552E-4 76-77 4.201268783172518E-4 78-79 6.919736819342971E-4 80-81 0.0016681508403773235 82-83 3.0067904036430764E-4 84-85 1.64755638555785E-5 86-87 9.473449216957639E-5 88-89 4.7779135181177663E-4 90-91 3.7481907771441095E-4 92-93 6.878547909704025E-4 94-95 0.0031427138054515996 96-97 1.31804510844628E-4 98-99 9.143937939846069E-4 100-101 0.0017793608964024783 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.213919E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.075163825779825 #Duplication Level Percentage of deduplicated Percentage of total 1 75.25225069567017 34.672597790616564 2 13.382397592472712 12.331923229098035 3 4.327054948077568 5.981092968474756 4 1.9201481763796886 3.5388456718586614 5 1.0555458920820489 2.4317224951654657 6 0.6483459899217079 1.7923588620858053 7 0.4267378791762809 1.3763412385599088 8 0.32467174511818947 1.1967443076737927 9 0.25784234838047115 1.0692115598568603 >10 2.2046787217306587 21.30777951904047 >50 0.18568532074879104 5.561087754096702 >100 0.013786344127713089 0.8883281454081925 >500 2.27160322161539E-4 0.0747183938326214 >1k 4.703904145748812E-4 0.5826076743032775 >5k 1.7421708591048547E-5 0.06851113441100255 >10k+ 1.3937366872838837E-4 7.126129255517988 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 678019 5.585372664897741 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 58328 0.4804933442840914 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 52998 0.43658596660897475 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 39338 0.3240578654753736 No Hit TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16759 0.13805698732782004 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15964 0.1315079506952276 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 13755 0.11331069041674116 No Hit GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12600 0.10379605229014456 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0014168984915797513 8.23778192778925E-6 0.0 0.0 8.23778192778925E-6 2 0.0014498496192909083 8.23778192778925E-6 0.0 0.0 8.23778192778925E-6 3 0.001474562965074276 8.23778192778925E-6 0.0 0.0 3.2951127711157E-5 4 0.0014992763108576438 8.23778192778925E-6 0.0 0.0 4.942669156673551E-5 5 0.0015157518747132223 8.23778192778925E-6 0.0 0.0 6.5902255422314E-5 6 0.0015898919120633254 8.23778192778925E-6 0.0 8.23778192778925E-6 7.414003735010327E-5 7 0.0016310808217022718 8.23778192778925E-6 0.0 8.23778192778925E-6 7.414003735010327E-5 8 0.0016475563855578503 8.23778192778925E-6 0.0 8.23778192778925E-6 7.414003735010327E-5 9 0.0017134586409801643 8.23778192778925E-6 0.0 8.23778192778925E-6 8.237781927789252E-5 10-11 0.0017958364602580568 1.64755638555785E-5 0.0 8.23778192778925E-6 9.885338313347102E-5 12-13 0.0018864520614637384 1.64755638555785E-5 0.0 8.23778192778925E-6 9.885338313347102E-5 14-15 0.0021953688837558355 2.0594454819473126E-5 0.0 8.23778192778925E-6 1.1532894698904952E-4 16-17 0.002648446889784244 2.8832236747262376E-5 0.0 8.23778192778925E-6 1.2768561988073338E-4 18-19 0.0028749858927984485 4.118890963894625E-5 0.0 8.23778192778925E-6 1.5651785662799578E-4 20-21 0.003043860422318128 4.942669156673551E-5 0.0 8.23778192778925E-6 1.6475563855578503E-4 22-23 0.003377490590393593 4.942669156673551E-5 0.0 8.23778192778925E-6 1.8123120241136354E-4 24-25 0.003707001867505163 5.766447349452475E-5 0.0 8.23778192778925E-6 1.8535009337525816E-4 26-27 0.004052988708472311 6.5902255422314E-5 0.0 8.23778192778925E-6 2.1418233012252054E-4 28-29 0.004378381094619987 7.414003735010327E-5 0.0 8.23778192778925E-6 2.224201120503098E-4 30-31 0.004996214739204181 7.414003735010327E-5 0.0 8.23778192778925E-6 2.471334578336775E-4 32-33 0.005391628271738065 9.061560120568177E-5 0.0 8.23778192778925E-6 2.9656014940041307E-4 34-35 0.005828230713910895 9.061560120568177E-5 0.0 8.23778192778925E-6 3.2539238614767544E-4 36-37 0.006273070938011514 9.885338313347102E-5 0.0 8.23778192778925E-6 3.4598684096714857E-4 38-39 0.006812645654281711 9.885338313347102E-5 0.0 8.23778192778925E-6 3.7481907771441095E-4 40-41 0.007788822812724737 9.885338313347102E-5 0.0 2.0594454819473126E-5 4.160079873533572E-4 42-43 0.008357229765742196 1.0297227409736564E-4 0.0 3.2951127711157E-5 4.283646602450411E-4 44-45 0.009185126849485015 1.0709116506126027E-4 0.0 3.2951127711157E-5 4.654346789200927E-4 46-47 0.010655570923595398 1.0709116506126027E-4 0.0 3.2951127711157E-5 5.066235885590388E-4 48-49 0.012245462835658721 1.1532894698904952E-4 0.0 3.2951127711157E-5 5.354558253063013E-4 50-51 0.013682955782057946 1.1532894698904952E-4 0.0 3.2951127711157E-5 5.642880620535637E-4 52-53 0.014811531906165073 1.1532894698904952E-4 0.0 3.2951127711157E-5 6.054769716925101E-4 54-55 0.016059555868225144 1.1532894698904952E-4 0.0 3.2951127711157E-5 6.178336445841939E-4 56-57 0.017245796465826798 1.2356672891683876E-4 0.0 3.2951127711157E-5 6.384280994036671E-4 58-59 0.018712121648973283 1.2768561988073338E-4 0.0 3.2951127711157E-5 6.837359000065079E-4 60-61 0.02036379692549503 1.31804510844628E-4 0.0 3.2951127711157E-5 7.414003735010326E-4 62-63 0.022773348139373387 1.31804510844628E-4 0.0 3.2951127711157E-5 7.619948283205057E-4 64-65 0.02525292049963795 1.3592340180852263E-4 0.0 3.2951127711157E-5 8.031837379594521E-4 66-67 0.02872926447316501 1.4416118373631188E-4 0.0 3.2951127711157E-5 8.237781927789251E-4 68-69 0.03316942893224342 1.4828007470020653E-4 0.0 3.2951127711157E-5 8.690859933817661E-4 70-71 0.039685514437124716 1.5239896566410116E-4 0.0 3.2951127711157E-5 9.061560120568177E-4 72-73 0.047997436402264074 1.5651785662799578E-4 0.0 3.2951127711157E-5 9.185126849485016E-4 74-75 0.05821640488368664 1.5651785662799578E-4 0.0 4.118890963894626E-5 9.349882488040801E-4 76-77 0.07033418209946463 1.5651785662799578E-4 0.0 4.118890963894626E-5 9.555827036235532E-4 78-79 0.08577178543214167 1.5651785662799578E-4 0.0 4.118890963894626E-5 9.844149403708154E-4 80-81 0.10520883189076041 1.5651785662799578E-4 0.0 4.118890963894626E-5 0.0010379605229014455 82-83 0.12916430173677157 1.5651785662799578E-4 0.0 4.118890963894626E-5 0.0010709116506126025 84-85 0.15989534721838936 1.5651785662799578E-4 0.0 4.118890963894626E-5 0.0011121005602515487 86-87 0.19823398431032052 1.6475563855578503E-4 0.0 4.118890963894626E-5 0.001120338342179338 88-89 0.2443161364143736 1.6475563855578503E-4 0.0 4.942669156673551E-5 0.0011615272518182844 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 6370 0.0 86.77909 1 TCGACGG 7010 0.0 79.282585 2 TATCTCG 10700 0.0 65.12629 2 ATATCTC 11075 0.0 63.174076 1 CGACGGC 9385 0.0 59.167725 3 ACGGCGC 10175 0.0 54.432472 5 GACGGCG 10510 0.0 52.698334 4 CGGCGCG 11260 0.0 49.228794 6 ATCTCGA 16880 0.0 41.254005 3 TCTCGAG 19900 0.0 34.94532 4 TCGAGGG 22930 0.0 30.368032 6 CTCGAGG 23055 0.0 30.3275 5 CGAGGGC 25305 0.0 27.592924 7 AGGGCGC 27295 0.0 25.616007 9 GAGGGCG 29450 0.0 23.773817 8 CGCGCCG 38675 0.0 20.375244 9 GGGCGCG 27190 0.0 20.229544 8 GGCGCGC 40670 0.0 19.410807 7 AATTCCC 6230 0.0 19.378515 1 ATTCCCC 6000 0.0 19.238226 2 >>END_MODULE