FastQCFastQC Report
Tue 31 May 2016
SRR1051267_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051267_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11448379
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT3750673.2761581355753506No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT980110.8561124679747237No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA315000.27514812359024804No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT166670.14558392939297346No Hit
GTCGACGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162160.14164450705204643No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT123510.10788426903057628No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA121030.10571802348611974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG1644000.094.591561
TCGACGG1657800.093.775492
CGACGGC1679150.092.432423
ACGGCGC1684000.092.140825
GACGGCG1688400.091.931654
CGGCGCG1691900.091.7262346
ATATCTC589800.090.7416841
TATCTCG602800.088.5562062
ATCTCGA629900.084.6800163
TCTCGAG644650.081.7157444
CTCGAGG664350.079.3641365
TCGAGGG672250.078.435126
CGAGGGC684900.076.6383367
AGGGCGC687450.076.623519
GAGGGCG708250.074.3866358
CGCGCCG2357200.066.176659
GGCGCGC2376600.065.7263957
GCGCGCC2375550.065.721468
CCATATT1724500.044.54914518-19
ATCCATA1730150.044.41601616-17
Produced by FastQC (version 0.11.5)