##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051267_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11448379 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14844931321718 33.0 31.0 34.0 31.0 34.0 2 32.398246511580375 34.0 31.0 34.0 31.0 34.0 3 32.50226018897523 34.0 31.0 34.0 31.0 34.0 4 36.00776599027688 37.0 37.0 37.0 35.0 37.0 5 35.94951101811007 37.0 35.0 37.0 35.0 37.0 6 36.0059133262447 37.0 37.0 37.0 35.0 37.0 7 35.956236074993676 37.0 37.0 37.0 35.0 37.0 8 35.94162038136578 37.0 36.0 37.0 35.0 37.0 9 37.60373106096505 39.0 38.0 39.0 35.0 39.0 10-11 37.6515564343214 39.0 38.0 39.0 35.0 39.0 12-13 37.597343781158884 39.0 38.0 39.0 35.0 39.0 14-15 39.06289523608539 41.0 39.0 41.0 36.0 41.0 16-17 38.95633390543762 40.5 38.5 41.0 35.5 41.0 18-19 38.940496903535426 40.5 39.0 41.0 35.0 41.0 20-21 38.97862981300672 40.5 39.0 41.0 35.0 41.0 22-23 38.858273953019896 40.5 38.5 41.0 35.0 41.0 24-25 38.81869345869839 40.0 39.0 41.0 35.0 41.0 26-27 38.8205431092035 40.0 38.5 41.0 35.0 41.0 28-29 38.626037319344505 40.0 38.0 41.0 35.0 41.0 30-31 37.944143489659105 39.5 36.0 41.0 34.0 41.0 32-33 37.586490672609635 39.0 35.0 41.0 34.0 41.0 34-35 37.46365258347929 39.0 35.0 41.0 34.0 41.0 36-37 37.3380078087911 39.0 35.0 41.0 33.0 41.0 38-39 36.86120240254101 39.0 35.0 41.0 32.0 41.0 40-41 35.26119911823325 39.0 35.0 41.0 23.0 41.0 42-43 35.301850899590235 39.0 34.5 41.0 23.0 41.0 44-45 35.27724117099897 39.0 34.0 41.0 23.0 41.0 46-47 35.50713970073842 39.0 34.0 41.0 23.5 41.0 48-49 35.63023210534871 39.0 34.0 41.0 24.0 41.0 50-51 35.26469712437019 38.5 33.5 40.5 24.0 41.0 52-53 35.168459482342435 38.0 34.0 40.0 24.0 41.0 54-55 35.076897305723364 38.0 34.0 40.5 24.5 41.0 56-57 34.71331491558762 38.0 34.0 40.0 25.0 41.0 58-59 34.342626803322986 37.0 34.0 40.0 24.5 41.0 60-61 33.92076725447332 36.5 33.5 40.0 23.0 41.0 62-63 33.650002939280746 36.0 33.0 40.0 22.0 41.0 64-65 33.32011160706682 35.5 33.0 39.0 21.5 41.0 66-67 32.928316532847134 35.0 33.0 39.0 20.0 41.0 68-69 32.59010738550846 35.0 33.0 38.5 19.5 40.5 70-71 32.22528267975754 35.0 33.0 37.0 19.0 39.5 72-73 31.850770576340985 35.0 32.5 37.0 18.5 39.0 74-75 31.49499295926524 35.0 32.0 36.0 18.0 39.0 76-77 31.164243820020282 35.0 32.0 36.0 16.0 37.5 78-79 30.87399259755464 35.0 32.0 35.0 11.5 37.0 80-81 30.587912926362762 35.0 32.0 35.0 8.5 37.0 82-83 30.36004787227956 35.0 31.5 35.0 7.0 36.0 84-85 30.14639618412353 35.0 31.0 35.0 6.0 36.0 86-87 29.94996951096745 35.0 31.0 35.0 2.0 36.0 88-89 29.770215853266212 35.0 31.0 35.0 2.0 35.0 90-91 29.582276364190946 35.0 31.0 35.0 2.0 35.0 92-93 29.36511033570779 35.0 31.0 35.0 2.0 35.0 94-95 29.15993705309721 35.0 31.0 35.0 2.0 35.0 96-97 28.90529716041022 35.0 30.5 35.0 2.0 35.0 98-99 28.600418190208412 35.0 30.0 35.0 2.0 35.0 100-101 27.62900961786817 34.0 27.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5056370220600357 1101 2 0.4806280387390274 1101 3 0.4876381987078773 1101 4 0.35636084126845446 1101 5 0.3753578020348911 1101 6 0.3483440397365811 1101 7 0.3722343664078238 1101 8 0.30880493578176527 1101 9 0.4955225960637506 1101 10-11 0.44629533823616896 1101 12-13 0.46036922251483503 1101 14-15 0.6111444163876243 1101 16-17 0.5300008101111615 1101 18-19 0.6116394613362814 1101 20-21 0.6368658284662203 1101 22-23 0.6640217726045563 1101 24-25 0.7044065964876651 1101 26-27 0.6360173328827372 1101 28-29 0.6350721646134829 1101 30-31 0.6644709690944097 1101 32-33 0.6545391335084645 1101 34-35 0.6239983988886451 1101 36-37 0.6020015243164352 1101 38-39 0.5768390555545082 1101 40-41 0.3970213777403728 1101 42-43 0.4012800719044449 1101 44-45 0.31251409272026365 1101 46-47 0.42026515736406367 1101 48-49 0.4012002400606889 1101 50-51 0.30157642843749954 1101 52-53 0.22423219546344342 1101 54-55 0.16643871708267 1101 56-57 0.2628094607355962 1101 58-59 0.17157568818990399 1101 60-61 0.17024329207426803 1101 62-63 0.23551532943772457 1101 64-65 0.22010932798344385 1101 66-67 0.27377328128945777 1101 68-69 0.20373952416236563 1101 70-71 0.1374705838428838 1101 72-73 0.13291244235735178 1101 74-75 0.16474081819392694 1101 76-77 0.1608020453766663 1101 78-79 0.07686695751861095 1101 80-81 0.08090594896915704 1101 82-83 0.09243455532885037 1101 84-85 0.09258536116260174 1101 86-87 0.008098822650705273 1101 88-89 0.0424338268515001 1101 90-91 -0.03129898879074844 1101 92-93 -0.042219328125494116 1101 94-95 0.022515990321554824 1101 96-97 -0.011919644296479959 1101 98-99 0.00834181159959968 1101 100-101 0.31399097476550253 1102 1 0.41136200043878546 1102 2 0.40501151634368426 1102 3 0.42335711713883484 1102 4 0.20982965718858537 1102 5 0.3060131303914275 1102 6 0.28999809564499657 1102 7 0.3477687147236068 1102 8 0.3261984808487739 1102 9 0.31689127484290935 1102 10-11 0.37856617321730823 1102 12-13 0.3844397149523857 1102 14-15 0.5615928446155749 1102 16-17 0.5355964717671213 1102 18-19 0.5580426280930126 1102 20-21 0.5492289805304367 1102 22-23 0.5995081344192101 1102 24-25 0.5796824439155586 1102 26-27 0.5479391276699204 1102 28-29 0.5476160560423509 1102 30-31 0.49752059088417155 1102 32-33 0.43495798365716354 1102 34-35 0.430778449417744 1102 36-37 0.433270866915052 1102 38-39 0.4013196364107756 1102 40-41 0.20099660892142168 1102 42-43 0.17263547152077763 1102 44-45 0.1681702736511923 1102 46-47 0.14271265589466253 1102 48-49 0.16168782817334204 1102 50-51 0.048668577500912136 1102 52-53 0.018192701934339084 1102 54-55 0.09003235407008958 1102 56-57 0.11165892197569605 1102 58-59 0.07933633098470949 1102 60-61 -0.05982383347559761 1102 62-63 0.0022096897106607116 1102 64-65 0.035463114662157125 1102 66-67 0.09225521114871071 1102 68-69 -0.06468614553432417 1102 70-71 -0.0031218341316474607 1102 72-73 -0.06235259259491244 1102 74-75 0.001625972054792868 1102 76-77 -0.07697157843752933 1102 78-79 -0.07729225957688257 1102 80-81 -0.057758215316265904 1102 82-83 -0.020797909594278963 1102 84-85 -0.05719580500666055 1102 86-87 -0.14089023605320605 1102 88-89 -0.08615659669770537 1102 90-91 -0.06448466769541383 1102 92-93 -0.05942247326753147 1102 94-95 -0.07879842317707997 1102 96-97 -0.02588714223936961 1102 98-99 -0.019646022176452504 1102 100-101 0.28230137105839503 1103 1 0.38477162413224164 1103 2 0.27205089327759424 1103 3 0.3090849397419504 1103 4 0.23483610818684753 1103 5 0.2714402847866708 1103 6 0.28373270167802644 1103 7 0.3178732735979608 1103 8 0.2792966829814034 1103 9 0.4173820929389507 1103 10-11 0.32607117742844594 1103 12-13 0.32496814191218704 1103 14-15 0.5089381397449486 1103 16-17 0.4901550851420211 1103 18-19 0.5200472922711725 1103 20-21 0.4736424135054236 1103 22-23 0.5594826192768494 1103 24-25 0.5678385428707244 1103 26-27 0.5381319988354321 1103 28-29 0.5214921760988247 1103 30-31 0.5309894517686189 1103 32-33 0.5101739451396838 1103 34-35 0.41484008001523875 1103 36-37 0.45284231387446994 1103 38-39 0.46386455522346637 1103 40-41 0.2472761616386947 1103 42-43 0.2336957688443988 1103 44-45 0.23241257780790647 1103 46-47 0.21992865487709423 1103 48-49 0.31809933497045506 1103 50-51 0.11045122446762434 1103 52-53 0.09658633242237613 1103 54-55 0.16498518019516695 1103 56-57 0.23267566619285418 1103 58-59 0.1088583946149626 1103 60-61 0.06461263660707317 1103 62-63 0.17008992867631179 1103 64-65 0.1469722833904754 1103 66-67 0.21649602797786827 1103 68-69 0.2052367034656868 1103 70-71 0.16266863866115955 1103 72-73 0.1623758877769248 1103 74-75 0.09729479310825795 1103 76-77 0.1517104567738734 1103 78-79 0.11963198502794725 1103 80-81 0.08663148376214735 1103 82-83 0.12293252330672644 1103 84-85 0.14541793374463197 1103 86-87 0.11588307140857168 1103 88-89 0.1358597127231853 1103 90-91 0.12626897917096258 1103 92-93 0.10850626727260959 1103 94-95 0.11883680200988422 1103 96-97 0.16705207547193623 1103 98-99 0.21717350392601986 1103 100-101 0.5647118146823473 1104 1 0.28339085858254265 1104 2 0.27474138985692775 1104 3 0.22047498688686318 1104 4 0.17551641646640093 1104 5 0.19736310855576988 1104 6 0.22421269540944877 1104 7 0.2080831309319109 1104 8 0.190918452846347 1104 9 0.2992119477729389 1104 10-11 0.2638246582598498 1104 12-13 0.2987747293261549 1104 14-15 0.3631719370647559 1104 16-17 0.37434283362291865 1104 18-19 0.3912940017357869 1104 20-21 0.40841988376234895 1104 22-23 0.4720640874293167 1104 24-25 0.4954423894622835 1104 26-27 0.3821781959933048 1104 28-29 0.41502596856795293 1104 30-31 0.4049688261051543 1104 32-33 0.416952172849399 1104 34-35 0.4117161655887429 1104 36-37 0.39974250361079555 1104 38-39 0.32611794463816324 1104 40-41 0.10384277364189387 1104 42-43 0.05508796614779499 1104 44-45 0.07932540730453752 1104 46-47 0.10581792964403292 1104 48-49 0.14607657220802395 1104 50-51 -2.608417926950324E-4 1104 52-53 -0.012599172617669296 1104 54-55 0.11187950247081346 1104 56-57 0.15022635116525862 1104 58-59 0.0867942891088731 1104 60-61 0.06389189508690407 1104 62-63 0.12059559837838663 1104 64-65 0.14060171360313944 1104 66-67 0.18205722867114105 1104 68-69 0.09224513339290752 1104 70-71 0.09985680038292344 1104 72-73 0.13169951601312135 1104 74-75 0.1375377283684287 1104 76-77 0.13269708567983685 1104 78-79 0.1029966752278888 1104 80-81 0.10901501606676334 1104 82-83 0.07058929364740862 1104 84-85 0.09735636925013935 1104 86-87 0.08501936533756904 1104 88-89 0.08253703223580189 1104 90-91 0.0677128315833393 1104 92-93 0.08754712401515263 1104 94-95 0.14051954122784238 1104 96-97 0.1720934088873598 1104 98-99 0.20921590262603473 1104 100-101 0.5465259234533164 1105 1 0.172117564769664 1105 2 0.05887734086651619 1105 3 0.1758389997549159 1105 4 0.11478227819250009 1105 5 0.11430605450356524 1105 6 0.15862145374570247 1105 7 0.14663755762434505 1105 8 0.1743439115930272 1105 9 0.17869880321737952 1105 10-11 0.10833438940763074 1105 12-13 0.18051010025735081 1105 14-15 0.27950842766929185 1105 16-17 0.2783195809763157 1105 18-19 0.2799352963989321 1105 20-21 0.22493562871483874 1105 22-23 0.29801139978792435 1105 24-25 0.30678871706484756 1105 26-27 0.27720431486930863 1105 28-29 0.29160691983727105 1105 30-31 0.3051291311733877 1105 32-33 0.2593408647328914 1105 34-35 0.23454219397526543 1105 36-37 0.3144708934173366 1105 38-39 0.21328496140904463 1105 40-41 0.07610024833901718 1105 42-43 0.06146166690344046 1105 44-45 0.0553870806446497 1105 46-47 -0.010939036720174045 1105 48-49 0.0722786822898982 1105 50-51 -0.11248726343634274 1105 52-53 -0.007759187634334808 1105 54-55 0.0549311550860665 1105 56-57 0.09891840629783388 1105 58-59 0.058388352318409886 1105 60-61 -0.013967063436503224 1105 62-63 0.012584768974882365 1105 64-65 0.033248734990088735 1105 66-67 0.05234687466752064 1105 68-69 0.03859076412904017 1105 70-71 0.038393847704938366 1105 72-73 0.03391195040442341 1105 74-75 0.08535034444837208 1105 76-77 0.074960602567959 1105 78-79 0.038681922482826536 1105 80-81 0.0197065357385533 1105 82-83 0.011871277867534502 1105 84-85 0.029209024371102288 1105 86-87 -0.0357460013410531 1105 88-89 -0.008828569768301264 1105 90-91 -0.07680327616054683 1105 92-93 2.9725275390291017E-4 1105 94-95 0.028293364044454705 1105 96-97 0.10187597356133438 1105 98-99 0.12826056413193143 1105 100-101 0.4070833912312892 1106 1 0.16520359754156289 1106 2 0.08353699521494207 1106 3 0.05426365325998006 1106 4 0.054242578691876986 1106 5 0.055360597442494 1106 6 0.08847852174446302 1106 7 0.11133696989805486 1106 8 0.1073810673395954 1106 9 0.05723053214251905 1106 10-11 0.13486483510345693 1106 12-13 0.1112324354261176 1106 14-15 0.19132186079647084 1106 16-17 0.1504543669666134 1106 18-19 0.2031196910446198 1106 20-21 0.13303361360356547 1106 22-23 0.15681353073624393 1106 24-25 0.2106409582633404 1106 26-27 0.20230154280740464 1106 28-29 0.19067126994271888 1106 30-31 0.19343629377044635 1106 32-33 0.16484262456399534 1106 34-35 0.11492421694256194 1106 36-37 0.11794342464296648 1106 38-39 0.13297826347785247 1106 40-41 0.04966710324876544 1106 42-43 -0.06380027768595653 1106 44-45 -0.011166524339316197 1106 46-47 0.0035647819121962243 1106 48-49 -0.0019138105769016533 1106 50-51 -0.19337309398132163 1106 52-53 -0.15283751040018956 1106 54-55 -0.031198430200930716 1106 56-57 -0.017696114337205415 1106 58-59 -0.02189649277061534 1106 60-61 -0.031686021841942136 1106 62-63 -0.036946424259419075 1106 64-65 0.023962948195084266 1106 66-67 0.04780833685003216 1106 68-69 0.034823882999944544 1106 70-71 0.036383919582817725 1106 72-73 0.06389334208461861 1106 74-75 0.07923844813371161 1106 76-77 0.005324752102854546 1106 78-79 -0.002666020837793326 1106 80-81 0.021362437113712218 1106 82-83 0.07015188329110345 1106 84-85 0.049742822995327174 1106 86-87 -0.022804648926953774 1106 88-89 0.016337663211139386 1106 90-91 0.0287607248687749 1106 92-93 0.04437634457702089 1106 94-95 0.09826027556378136 1106 96-97 0.05598766166404445 1106 98-99 0.04514348384364553 1106 100-101 0.38435585220338453 1107 1 0.042847622645581396 1107 2 -0.01488779938335938 1107 3 -0.010893629647128478 1107 4 -0.04335470799482266 1107 5 -0.1350994970310424 1107 6 -0.1086722164358278 1107 7 -0.016694748453154773 1107 8 -0.07279397799383247 1107 9 -0.08421627013563437 1107 10-11 -0.02722715071273285 1107 12-13 -0.05632661552392193 1107 14-15 -0.06913158192030977 1107 16-17 -0.039144799051328505 1107 18-19 -0.00350052263598144 1107 20-21 -0.04481723441683272 1107 22-23 -0.06383941253967151 1107 24-25 0.01606496241939226 1107 26-27 -0.07295438239815155 1107 28-29 -0.09227032376160338 1107 30-31 -0.08212212219689263 1107 32-33 -0.08652205203373597 1107 34-35 -0.12178008852650635 1107 36-37 -0.14721637904170848 1107 38-39 -0.3900340653055707 1107 40-41 -0.3783922788157952 1107 42-43 -0.32507954790912663 1107 44-45 -0.1893340608482248 1107 46-47 -0.40973694544320693 1107 48-49 -0.2368201427192247 1107 50-51 -0.26138074981244586 1107 52-53 -0.2417119638161438 1107 54-55 -0.0738094336789814 1107 56-57 -0.0735530248251095 1107 58-59 -0.18507358413148722 1107 60-61 -0.21554605918034753 1107 62-63 -0.23627765455373861 1107 64-65 -0.10667785131850138 1107 66-67 0.013884735553304495 1107 68-69 -0.011155875497379952 1107 70-71 -0.03778287966612481 1107 72-73 -0.008511899406823176 1107 74-75 -0.002846862671624706 1107 76-77 0.02132201797832778 1107 78-79 -0.0807267085262815 1107 80-81 -0.08175975289655923 1107 82-83 -0.07144712550881138 1107 84-85 -0.10196119904539813 1107 86-87 -0.10598841431419359 1107 88-89 -0.11916308970205591 1107 90-91 -0.1585107090443536 1107 92-93 -0.03184169901265932 1107 94-95 -0.03733692072729866 1107 96-97 -0.07422782728875887 1107 98-99 -0.08859885146321744 1107 100-101 0.26025435129463403 1108 1 -0.25306154426059635 1108 2 -0.1675841169760801 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46-47 -0.7635315700086664 2308 48-49 -0.5980678823274985 2308 50-51 -1.060125213872908 2308 52-53 -0.9507616388860214 2308 54-55 -0.5813382798092945 2308 56-57 -0.6105965358309007 2308 58-59 -0.8597470906061702 2308 60-61 -0.9118566023807375 2308 62-63 -0.8692016889349006 2308 64-65 -0.9991709983028088 2308 66-67 -0.6508823750012525 2308 68-69 -0.5421291222996985 2308 70-71 -0.44583677343060657 2308 72-73 -0.47985593231446444 2308 74-75 -0.7404955071370622 2308 76-77 -0.5717629028722016 2308 78-79 -0.5179360097233392 2308 80-81 -0.5635284672774787 2308 82-83 -0.5297100530252905 2308 84-85 -0.5075388525049718 2308 86-87 -0.44115893648525173 2308 88-89 -0.5848271328078845 2308 90-91 -0.6698453619645655 2308 92-93 -0.6566202698703663 2308 94-95 -0.6291031281800947 2308 96-97 -0.6232128758529463 2308 98-99 -0.6524147155035713 2308 100-101 -0.12088678289349275 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 155.0 11 1199.0 12 3952.0 13 7819.0 14 11405.0 15 15533.0 16 20977.0 17 34665.0 18 83665.0 19 216804.0 20 229590.0 21 149523.0 22 125242.0 23 121304.0 24 125098.0 25 141215.0 26 169875.0 27 195915.0 28 216856.0 29 234021.0 30 252109.0 31 280603.0 32 325958.0 33 400676.0 34 550989.0 35 847160.0 36 1462350.0 37 2620891.0 38 2285767.0 39 316853.0 40 208.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.0045208347546 29.41440012746212 17.534794180382836 18.04628485740044 2 20.638977228271003 23.399988433030302 38.17847834242179 17.7825559962769 3 21.02848847778048 25.296610661779766 20.275240088642736 33.399660771797016 4 35.59291800391697 20.095784101848242 24.666105269042824 19.645192625191964 5 20.949139885821292 35.447700793114926 19.463948340647093 24.139210980416685 6 20.95789171266201 20.265731918866038 22.90766861409674 35.868707754375215 7 35.49131852317717 20.23857846958808 19.457094877153445 24.8130081300813 8 39.27740337736897 20.66313492940791 19.325417161678523 20.734044531544598 9 20.558194308556697 24.53276573041476 19.71867807660805 35.1903618844205 10-11 32.593762837516124 20.24743415639891 19.66049953447558 27.498303471609386 12-13 30.01968226244082 20.085249623549327 19.86639767953175 30.028670434478105 14-15 29.82406068142922 20.089769914151166 19.978963834093893 30.107205570325718 16-17 29.794043331374688 27.418475576323946 19.84470465207345 22.942776440227913 18-19 22.847500943146624 20.17404385371938 27.126608928652697 29.851846274481304 20-21 22.371250113225635 29.62793247847577 20.501059582321655 27.499757825976936 22-23 19.90201407552982 27.170632628427132 30.07231853522669 22.855034760816356 24-25 19.91643096371984 20.01289003447562 37.49401989574245 22.57665910606209 26-27 19.816630808606178 20.00136001786803 40.121784926931575 20.060224246594213 28-29 19.9260349434623 20.01984298388444 40.165917812469345 19.888204260183908 30-31 19.915173143726285 19.905276546138104 40.16396993845155 20.01558037168406 32-33 19.861417719276652 19.940757086058884 40.247136493496974 19.950688701167486 34-35 19.885637948013247 20.05427319049039 40.15967589469879 19.900412966797568 36-37 19.953920987416645 19.96878335352105 40.015407421434944 20.061888237627354 38-39 20.22492976922658 20.253453582614544 38.80855887352644 20.71305777463244 40-41 22.26647108730415 22.20979057384456 32.49886730689122 23.02487103196007 42-43 22.468495556847056 22.59614719000226 32.91458578010246 22.020771473048224 44-45 22.721179326085156 23.75741168929783 31.654439379258108 21.866969605358907 46-47 22.164762365047487 24.890764011219403 31.16532043532102 21.77915318841209 48-49 21.995917500634807 25.410641104736314 30.653400800235563 21.94004059439332 50-51 21.959775862897274 25.44051326173641 30.492935663217846 22.106775212148474 52-53 21.924428354277705 25.185006248109975 30.801002807238337 22.08956259037398 54-55 21.980473125981312 24.60290042429788 31.09822179227092 22.31840465744989 56-57 21.890038728698038 24.299170239085814 31.521865406375305 22.288925625840843 58-59 22.026424272239343 24.27986490266098 31.409013255897932 22.284697569201747 60-61 22.252504889584127 24.29852412978811 30.882740432996524 22.56623054763124 62-63 22.519660463014272 24.595226018397593 30.129052724515564 22.75606079407257 64-65 22.670773788539098 24.975743004778725 29.397456064004224 22.95602714267795 66-67 22.812032693855464 25.0796968743084 28.797665745410534 23.310604686425606 68-69 22.9337445938853 25.37827844448546 28.339282792786648 23.34869416884259 70-71 23.135650907434144 25.737355480631802 27.92483547234067 23.202158139593386 72-73 23.296835298691633 25.71501170602406 27.702454644452285 23.285698350832025 74-75 23.301650628341815 25.725620390857788 27.660295098655197 23.312433882145196 76-77 23.35694047403481 25.813468473862905 27.502318917219615 23.327272134882673 78-79 23.45208819185557 25.543269683279856 27.505143418444415 23.49949870642016 80-81 23.37635525653019 25.51267756626821 27.598541965996183 23.51242521120542 82-83 23.529754484443448 25.614590326326404 27.444194250186854 23.411460939043295 84-85 23.58330242987849 25.405644695274752 27.440350581661182 23.570702293185573 86-87 23.58222730484039 25.42077038002936 27.47772394674851 23.519278368381734 88-89 23.67420509345528 25.544188577646683 27.2637849568783 23.517821372019736 90-91 23.83827274437755 25.372165715157223 27.126972571755413 23.662588968709812 92-93 23.836437780486584 25.450587824274272 27.030696545985073 23.68227784925407 94-95 23.904448938630658 25.55779769890406 26.85795210515103 23.67980125731425 96-97 23.8536171802139 25.461901636904226 26.782704346178615 23.901776836703256 98-99 23.84064064280908 25.489543097476503 26.796585487312193 23.87323077240223 100-101 23.89817523809507 25.509145865171167 26.686550523524616 23.906128373209143 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 30626.0 1 17338.5 2 2984.5 3 1621.5 4 1104.5 5 812.5 6 702.0 7 640.5 8 581.0 9 554.5 10 603.0 11 831.0 12 1294.5 13 2327.5 14 3368.5 15 7550.0 16 125976.0 17 143827.5 18 44431.0 19 36515.5 20 27593.0 21 22230.0 22 20951.5 23 22315.0 24 25087.5 25 29259.0 26 35286.5 27 43737.0 28 53389.5 29 64149.5 30 78442.5 31 95108.5 32 113515.0 33 135334.5 34 158179.5 35 179543.0 36 202509.0 37 223085.5 38 241344.5 39 258698.5 40 270126.5 41 276646.0 42 286096.5 43 304291.5 44 327375.5 45 352519.0 46 378897.0 47 405773.5 48 432790.0 49 453598.5 50 457069.0 51 460447.0 52 473231.5 53 482300.5 54 478555.5 55 459490.5 56 429747.5 57 392886.0 58 352294.0 59 311417.0 60 270931.5 61 229725.0 62 187205.0 63 143945.0 64 105430.5 65 74972.0 66 53377.5 67 39922.5 68 30157.5 69 22469.5 70 17318.0 71 13214.0 72 10430.5 73 7692.5 74 5532.0 75 4413.0 76 3290.5 77 2080.5 78 1237.5 79 693.5 80 320.5 81 142.0 82 68.0 83 29.5 84 19.5 85 16.0 86 10.0 87 6.0 88 5.0 89 7.0 90 4.5 91 1.5 92 1.5 93 1.0 94 0.5 95 0.5 96 1.0 97 2.0 98 2.0 99 2.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002375882210049126 2 0.017408578105249657 3 0.005485492749672247 4 0.006629759549365023 5 0.010796288278017352 6 0.002620458319907124 7 0.0013451686042189903 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 7.861374959721372E-4 34-35 8.73486106635708E-6 36-37 0.0 38-39 4.36743053317854E-4 40-41 0.0 42-43 2.926178457229622E-4 44-45 6.813191631758523E-4 46-47 0.0 48-49 0.0 50-51 2.533109709243553E-4 52-53 0.0010219787447637784 54-55 4.716824975832823E-4 56-57 1.8343208239349868E-4 58-59 3.5812930372064027E-4 60-61 0.0027907881107010874 62-63 0.007411529614803982 64-65 0.0011792062439582058 66-67 0.006852498506557129 68-69 0.0 70-71 0.0 72-73 0.0 74-75 6.289099967777097E-4 76-77 0.0012927594378208478 78-79 0.0043368585194462905 80-81 0.0019522414483308076 82-83 0.0014281497843493826 84-85 7.905049265053158E-4 86-87 0.0018430556850013438 88-89 0.002240491863520591 90-91 0.002209919849788341 92-93 0.00460763921250336 94-95 6.987888853085664E-5 96-97 0.0 98-99 0.0011136947859605277 100-101 5.546636777136746E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.1448379E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.21174287219589 #Duplication Level Percentage of deduplicated Percentage of total 1 73.61523573802565 32.54657873925676 2 13.555348595262767 11.986111732734784 3 4.548738944897404 6.033230298736431 4 2.20319987206911 3.896292249598975 5 1.1737796605611 2.5947422270670364 6 0.7643727657057059 2.027655130553394 7 0.5639452760364283 1.7453102476678468 8 0.41232316549553866 1.4583620618510902 9 0.32924658738945706 1.3100908916889582 >10 2.634070303763307 23.07626535502403 >50 0.1778345301582306 5.0955753351771795 >100 0.018948143581390936 1.3758191349345648 >500 0.0012193607012137508 0.35961836746760495 >1k 0.0014668490563567512 1.2759840830839757 >5k 1.351036481946162E-4 0.42348906073809434 >10k+ 1.351036481946162E-4 4.794875084419316 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 375067 3.2761581355753506 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT 98011 0.8561124679747237 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 31500 0.27514812359024804 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 16667 0.14558392939297346 No Hit GTCGACGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16216 0.14164450705204643 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12351 0.10788426903057628 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA 12103 0.10571802348611974 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.003860808591329829 8.73486106635708E-6 0.0 0.0 8.73486106635708E-6 2 0.0038695434523961865 1.746972213271416E-5 0.0 0.0 1.746972213271416E-5 3 0.0038957480355952577 1.746972213271416E-5 0.0 0.0 1.746972213271416E-5 4 0.0039044828966616147 2.620458319907124E-5 0.0 0.0 3.493944426542832E-5 5 0.003965626924126114 3.493944426542832E-5 0.0 0.0 5.240916639814248E-5 6 0.003983096646258829 3.493944426542832E-5 0.0 0.0 7.861374959721372E-5 7 0.004035505812656971 3.493944426542832E-5 0.0 0.0 8.73486106635708E-5 8 0.0041141195622541845 4.36743053317854E-5 0.0 8.73486106635708E-6 8.73486106635708E-5 9 0.004175263589718684 4.36743053317854E-5 0.0 8.73486106635708E-6 9.608347172992787E-5 10-11 0.004284449353048147 5.6776596931321016E-5 0.0 8.73486106635708E-6 1.1355319386264203E-4 12-13 0.004363063102645361 7.861374959721372E-5 0.0 8.73486106635708E-6 1.4412520759489182E-4 14-15 0.004620741504102895 8.298118013039225E-5 0.0 1.746972213271416E-5 1.7032979079396307E-4 16-17 0.004917726780359037 8.73486106635708E-5 0.0 2.620458319907124E-5 1.965343739930343E-4 18-19 0.005105526293285713 1.0045090226310641E-4 0.0 2.620458319907124E-5 2.096366655925699E-4 20-21 0.00532389781994464 1.1355319386264203E-4 0.0 3.493944426542832E-5 2.4894354039117674E-4 22-23 0.005516064763404495 1.1792062439582057E-4 0.0 3.493944426542832E-5 2.9261784572296216E-4 24-25 0.005703864276331173 1.2665548546217766E-4 0.0 3.493944426542832E-5 3.1882242892203343E-4 26-27 0.0060008495525873135 1.5286006866124888E-4 0.0 3.493944426542832E-5 3.7559902585335446E-4 28-29 0.006306569689909811 1.7906465186032014E-4 0.0 3.493944426542832E-5 3.9743617851924716E-4 30-31 0.006738945312694487 1.8343208239349868E-4 0.0 3.493944426542832E-5 4.105384701187828E-4 32-33 0.007158218643879628 1.9216694345985575E-4 0.0 3.930687479860686E-5 4.411104838510326E-4 34-35 0.007603696558263838 1.9216694345985575E-4 0.0 4.36743053317854E-5 4.5858020598374665E-4 36-37 0.007948723570384942 1.9216694345985575E-4 0.0 4.36743053317854E-5 5.109893723818892E-4 38-39 0.008446610651167297 1.9216694345985575E-4 0.0 5.240916639814248E-5 5.721333998463887E-4 40-41 0.009219645855539898 1.9216694345985575E-4 4.36743053317854E-6 5.6776596931321016E-5 5.896031219791029E-4 42-43 0.009796146685919466 2.052692350593914E-4 8.73486106635708E-6 6.114402746449956E-5 6.245425662445311E-4 44-45 0.010394484668964924 2.18371526658927E-4 8.73486106635708E-6 6.114402746449956E-5 7.031563158417449E-4 46-47 0.011372789108396918 2.2710638772528407E-4 8.73486106635708E-6 6.114402746449956E-5 7.380957601071732E-4 48-49 0.01247338160275791 2.445761098579982E-4 8.73486106635708E-6 6.114402746449956E-5 7.817700654389587E-4 50-51 0.013346867709393619 2.620458319907124E-4 8.73486106635708E-6 6.114402746449956E-5 8.036072181048514E-4 52-53 0.014556645967084074 2.7951555412342654E-4 8.73486106635708E-6 6.987888853085664E-5 8.429140929034582E-4 54-55 0.015500010962250638 2.7951555412342654E-4 8.73486106635708E-6 7.861374959721372E-5 8.778535371688866E-4 56-57 0.01653509199861395 2.7951555412342654E-4 8.73486106635708E-6 7.861374959721372E-5 9.564672867661002E-4 58-59 0.017364903799917876 2.7951555412342654E-4 8.73486106635708E-6 7.861374959721372E-5 0.0010088764531642428 60-61 0.018373780253082117 2.8825041518978364E-4 8.73486106635708E-6 7.861374959721372E-5 0.0010612856195623853 62-63 0.01977572545423243 2.8825041518978364E-4 8.73486106635708E-6 7.861374959721372E-5 0.0010962250638278134 64-65 0.021789110930027736 2.8825041518978364E-4 8.73486106635708E-6 7.861374959721372E-5 0.0011355319386264205 66-67 0.024702187095657822 2.8825041518978364E-4 8.73486106635708E-6 8.73486106635708E-5 0.0011617365218254917 68-69 0.028379563604594153 2.9261784572296216E-4 8.73486106635708E-6 8.73486106635708E-5 0.001222880549289991 70-71 0.03452017093424318 3.057201373224978E-4 8.73486106635708E-6 8.73486106635708E-5 0.0012665548546217766 72-73 0.04166965471705645 3.188224289220334E-4 8.73486106635708E-6 1.0045090226310641E-4 0.0012883920072876693 74-75 0.04937816960811657 3.3192472052156905E-4 8.73486106635708E-6 1.0481833279628495E-4 0.0013233314515530975 76-77 0.05951060844509079 3.3629215105474757E-4 8.73486106635708E-6 1.091857633294635E-4 0.0013888429095507758 78-79 0.0730409082368779 3.4065958158792614E-4 8.73486106635708E-6 1.1355319386264203E-4 0.0014281497843493826 80-81 0.08939256815309836 3.4065958158792614E-4 8.73486106635708E-6 1.1355319386264203E-4 0.0014761915202143466 82-83 0.11068815943287691 3.493944426542832E-4 8.73486106635708E-6 1.1355319386264203E-4 0.0015591727003447387 84-85 0.1370062958258108 3.5812930372064027E-4 8.73486106635708E-6 1.1792062439582057E-4 0.0015984795751433456 86-87 0.16990615003224474 3.5812930372064027E-4 8.73486106635708E-6 1.222880549289991E-4 0.0016465213110083096 88-89 0.2097240142032335 3.7123159532017594E-4 8.73486106635708E-6 1.222880549289991E-4 0.0016901956163400949 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 164400 0.0 94.59156 1 TCGACGG 165780 0.0 93.77549 2 CGACGGC 167915 0.0 92.43242 3 ACGGCGC 168400 0.0 92.14082 5 GACGGCG 168840 0.0 91.93165 4 CGGCGCG 169190 0.0 91.726234 6 ATATCTC 58980 0.0 90.741684 1 TATCTCG 60280 0.0 88.556206 2 ATCTCGA 62990 0.0 84.680016 3 TCTCGAG 64465 0.0 81.715744 4 CTCGAGG 66435 0.0 79.364136 5 TCGAGGG 67225 0.0 78.43512 6 CGAGGGC 68490 0.0 76.638336 7 AGGGCGC 68745 0.0 76.62351 9 GAGGGCG 70825 0.0 74.386635 8 CGCGCCG 235720 0.0 66.17665 9 GGCGCGC 237660 0.0 65.726395 7 GCGCGCC 237555 0.0 65.72146 8 CCATATT 172450 0.0 44.549145 18-19 ATCCATA 173015 0.0 44.416016 16-17 >>END_MODULE