FastQCFastQC Report
Tue 31 May 2016
SRR1051267_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051267_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11448379
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1856871.621950146828647No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT287950.2515203244057521No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT185460.1619967333366584No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT184690.16132414903454892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG43000.084.574711
TCGACGG48700.074.619062
TATCTCG48150.051.6954462
CGACGGC71250.051.401463
ACGGCGC75700.048.1899075
ATATCTC53000.047.4490241
GACGGCG81900.044.717194
CGGCGCG89450.040.7812276
ATCTCGA84300.036.034691
AATTCGA42450.028.2208631
TCTCGAG108250.027.9091452
TCGAGGG126400.024.314274
CTCGAGG127450.023.7786253
CGCGCCG214450.022.1269079
CGAGGGC142350.021.6226185
GGGCGCG143250.021.3205138
AATTCCC65600.020.8706591
AGGGCGC149850.020.6667487
ATTCGAT9450.020.609592
GGCGCGC237500.020.099437
Produced by FastQC (version 0.11.5)