##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051267_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11448379 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.551920407247174 34.0 31.0 34.0 31.0 34.0 2 32.80551508645897 34.0 31.0 34.0 31.0 34.0 3 32.893822260775956 34.0 31.0 34.0 31.0 34.0 4 36.33225690728792 37.0 37.0 37.0 35.0 37.0 5 36.22017824532189 37.0 37.0 37.0 35.0 37.0 6 36.197326887937585 37.0 37.0 37.0 35.0 37.0 7 36.17897529423161 37.0 37.0 37.0 35.0 37.0 8 36.16748703025992 37.0 37.0 37.0 35.0 37.0 9 37.90328665743858 39.0 38.0 39.0 35.0 39.0 10-11 37.8873875069999 39.0 38.0 39.0 35.0 39.0 12-13 37.870182058088744 39.0 38.0 39.0 35.0 39.0 14-15 39.345637404212425 41.0 39.0 41.0 36.0 41.0 16-17 39.31551811832924 41.0 39.0 41.0 36.0 41.0 18-19 39.28447752297509 41.0 39.0 41.0 36.0 41.0 20-21 39.278926999184776 41.0 39.0 41.0 36.0 41.0 22-23 39.23409501904156 41.0 39.0 41.0 35.5 41.0 24-25 39.17799939187897 40.5 39.0 41.0 35.0 41.0 26-27 39.057328989545155 40.0 38.5 41.0 35.0 41.0 28-29 38.91816286829777 40.0 38.0 41.0 35.0 41.0 30-31 38.80450800065232 40.0 38.0 41.0 35.0 41.0 32-33 38.74591201950949 40.0 38.0 41.0 35.0 41.0 34-35 38.653124953323086 40.0 38.0 41.0 35.0 41.0 36-37 38.56404972267253 40.0 38.0 41.0 35.0 41.0 38-39 38.463052804244164 40.0 38.0 41.0 34.0 41.0 40-41 38.507803768550986 40.0 38.0 41.0 35.0 41.0 42-43 38.49781611003619 40.0 38.0 41.0 34.0 41.0 44-45 38.42602625227554 40.0 38.0 41.0 34.0 41.0 46-47 38.30860661583618 40.0 37.5 41.0 34.0 41.0 48-49 38.220922062415994 40.0 37.0 41.0 34.0 41.0 50-51 38.15138903070907 40.0 37.0 41.0 34.0 41.0 52-53 37.95938027558312 40.0 36.5 41.0 33.0 41.0 54-55 37.75496229640895 39.5 36.0 41.0 33.0 41.0 56-57 37.525053677904964 39.0 35.5 41.0 33.0 41.0 58-59 37.25661349087063 39.0 35.0 41.0 33.0 41.0 60-61 36.92228292756555 38.5 35.0 40.5 33.0 41.0 62-63 36.559284768612216 37.5 35.0 40.0 32.0 41.0 64-65 36.19873372466093 37.0 35.0 40.0 32.0 41.0 66-67 35.789805089436676 36.5 35.0 39.0 31.5 41.0 68-69 35.382459429409174 36.0 35.0 39.0 31.0 41.0 70-71 35.04089727462726 35.0 35.0 38.5 31.0 40.0 72-73 34.66141018741605 35.0 34.0 37.0 31.0 39.5 74-75 34.31339083026514 35.0 34.0 37.0 30.5 39.0 76-77 33.66355040307453 35.0 33.5 36.0 29.5 38.5 78-79 33.72776172067678 35.0 34.0 36.0 30.0 37.0 80-81 33.55960254285782 35.0 34.0 35.5 30.0 37.0 82-83 33.35658222880287 35.0 34.0 35.0 30.0 36.5 84-85 33.16010262238872 35.0 34.0 35.0 30.0 36.0 86-87 32.99390066488889 35.0 34.0 35.0 29.5 36.0 88-89 32.82709268272827 35.0 34.0 35.0 29.0 36.0 90-91 32.68999178835711 35.0 34.0 35.0 29.0 35.0 92-93 32.54381498026926 35.0 33.0 35.0 29.0 35.0 94-95 32.39298598517746 35.0 33.0 35.0 29.0 35.0 96-97 32.224282800211284 35.0 33.0 35.0 29.0 35.0 98-99 32.01815230785075 35.0 33.0 35.0 28.0 35.0 100-101 31.272877802176186 34.5 32.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3822441033261015 1101 2 0.3652450431748875 1101 3 0.3451470901145868 1101 4 0.21717264527966051 1101 5 0.24350738685517115 1101 6 0.28879083887977686 1101 7 0.3259896761389882 1101 8 0.3177954690396376 1101 9 0.4231675091150251 1101 10-11 0.3908668885401312 1101 12-13 0.3924330503677851 1101 14-15 0.5081839651752986 1101 16-17 0.5202694919946396 1101 18-19 0.4501313222824521 1101 20-21 0.38888464658099053 1101 22-23 0.42406058658229284 1101 24-25 0.4122682255230856 1101 26-27 0.3887517004301557 1101 28-29 0.43805805461365566 1101 30-31 0.4211790734701353 1101 32-33 0.39700999010554483 1101 34-35 0.35589529078369964 1101 36-37 0.29580107301499936 1101 38-39 0.25220265358844074 1101 40-41 0.40760867055203676 1101 42-43 0.38749602157201224 1101 44-45 0.40591957789332866 1101 46-47 0.38819231783675434 1101 48-49 0.3971529601753403 1101 50-51 0.3742437746657288 1101 52-53 0.36462460461235224 1101 54-55 0.3611236159329607 1101 56-57 0.31939066225571366 1101 58-59 0.2627062928677688 1101 60-61 0.30790620105196354 1101 62-63 0.18626400269462806 1101 64-65 0.19214856628283172 1101 66-67 0.22082044218037566 1101 68-69 0.2170879177204057 1101 70-71 0.18095614685616823 1101 72-73 0.18917409860034695 1101 74-75 0.18531627142044016 1101 76-77 -0.055936179062719305 1101 78-79 0.0755954160272907 1101 80-81 0.06707719583467764 1101 82-83 0.06645963261974686 1101 84-85 0.08096327189894481 1101 86-87 -0.04518762316846647 1101 88-89 -0.06901035106515963 1101 90-91 -0.061638696920113034 1101 92-93 -0.1278723080298647 1101 94-95 -0.16464419229703253 1101 96-97 -0.1819284814631743 1101 98-99 -0.1850434532647114 1101 100-101 -0.012955948407661566 1102 1 0.370804863691788 1102 2 0.31661144602740876 1102 3 0.2987126057650755 1102 4 0.21955702696127588 1102 5 0.215803754287883 1102 6 0.28258931333110127 1102 7 0.23975793289229586 1102 8 0.2886665431894002 1102 9 0.37914002758623155 1102 10-11 0.3478548950923397 1102 12-13 0.3427763277548408 1102 14-15 0.42096252411833746 1102 16-17 0.35857620190237327 1102 18-19 0.40224961275526994 1102 20-21 0.3197382057923548 1102 22-23 0.34033548025751514 1102 24-25 0.3374344243381131 1102 26-27 0.36759377837329765 1102 28-29 0.4091329292696244 1102 30-31 0.3785163347592615 1102 32-33 0.3262218040646374 1102 34-35 0.2623796604955757 1102 36-37 0.2658776057617587 1102 38-39 0.29578734783220995 1102 40-41 0.37121141369668464 1102 42-43 0.36706640244666744 1102 44-45 0.3148287496393323 1102 46-47 0.36040612075734657 1102 48-49 0.34754057337983113 1102 50-51 0.334263172591875 1102 52-53 0.37205965338475977 1102 54-55 0.3434164855689019 1102 56-57 0.33592059528048424 1102 58-59 0.2802299979028007 1102 60-61 0.28683216490700403 1102 62-63 0.30344517918222635 1102 64-65 0.229773797596728 1102 66-67 0.24772807131116537 1102 68-69 0.22278756059046145 1102 70-71 0.2692709480749471 1102 72-73 0.30038423563766514 1102 74-75 0.24025775357184642 1102 76-77 0.06372643167427583 1102 78-79 0.2705208971829265 1102 80-81 0.19625083011201383 1102 82-83 0.1895525763275927 1102 84-85 0.2215925136235981 1102 86-87 0.22121170710100557 1102 88-89 0.1639419290919122 1102 90-91 0.13151702333102122 1102 92-93 0.24779730121915833 1102 94-95 0.14778816602742495 1102 96-97 0.11082073304748974 1102 98-99 0.10946657729034115 1102 100-101 0.4441395217831712 1103 1 0.36000406828826215 1103 2 0.31789699366293434 1103 3 0.29996139335240457 1103 4 0.1824235100472933 1103 5 0.2128836767340161 1103 6 0.22311898944335695 1103 7 0.22021937436861805 1103 8 0.26949601082241514 1103 9 0.3422276638654651 1103 10-11 0.3621416847857404 1103 12-13 0.35444622160981964 1103 14-15 0.4440003564613022 1103 16-17 0.4020737856918686 1103 18-19 0.38935795658688477 1103 20-21 0.3402415762235691 1103 22-23 0.34539470984717013 1103 24-25 0.34317284573373286 1103 26-27 0.3617149147422296 1103 28-29 0.3070644189586673 1103 30-31 0.32666552916995073 1103 32-33 0.3216601881630581 1103 34-35 0.34260174358524154 1103 36-37 0.18307212844362653 1103 38-39 0.21127684583001383 1103 40-41 0.24918138133527634 1103 42-43 0.261099386152452 1103 44-45 0.25796365100949004 1103 46-47 0.2211663858513191 1103 48-49 0.28555124109108476 1103 50-51 0.3324571641939258 1103 52-53 0.34721256687449653 1103 54-55 0.37433459741796327 1103 56-57 0.36507815723895476 1103 58-59 0.30892373916760363 1103 60-61 0.2995486306113122 1103 62-63 0.2962202080926346 1103 64-65 0.28703256674828737 1103 66-67 0.2604418258732366 1103 68-69 0.3256110074606937 1103 70-71 0.3233174621541366 1103 72-73 0.30134364046339357 1103 74-75 0.3000684220939078 1103 76-77 0.16193247645727382 1103 78-79 0.31769014289755404 1103 80-81 0.33446815780752814 1103 82-83 0.3376182650236004 1103 84-85 0.3265243702036713 1103 86-87 0.24996812389326806 1103 88-89 0.2617696327088339 1103 90-91 0.21727597592808934 1103 92-93 0.26639312970875295 1103 94-95 0.27773860280003504 1103 96-97 0.25576827371209276 1103 98-99 0.29547888739636363 1103 100-101 0.5454201564235639 1104 1 0.2986927048172632 1104 2 0.26537019830391984 1104 3 0.23250673324535143 1104 4 0.1705756610453193 1104 5 0.18319180703828408 1104 6 0.19669410133555232 1104 7 0.22321097709022553 1104 8 0.21311225090883568 1104 9 0.2787016371531337 1104 10-11 0.3079360074345132 1104 12-13 0.30070048228179047 1104 14-15 0.33323847275455876 1104 16-17 0.35186753372127555 1104 18-19 0.28175398406413876 1104 20-21 0.2888996721066448 1104 22-23 0.3068215568846142 1104 24-25 0.30036716875563485 1104 26-27 0.3177805118333126 1104 28-29 0.30474379628024195 1104 30-31 0.28309799646982015 1104 32-33 0.26970361856601954 1104 34-35 0.27340315569366425 1104 36-37 0.20791353443272698 1104 38-39 0.2251011568931176 1104 40-41 0.24876161069794733 1104 42-43 0.26621986032536427 1104 44-45 0.27490109772193705 1104 46-47 0.2638516306335674 1104 48-49 0.27333751533750217 1104 50-51 0.28221024111269344 1104 52-53 0.24323220840902593 1104 54-55 0.25022553261097613 1104 56-57 0.27504931899616736 1104 58-59 0.2584259378329179 1104 60-61 0.2626380538837694 1104 62-63 0.3148418678843967 1104 64-65 0.3298648292000834 1104 66-67 0.2864661082053672 1104 68-69 0.3278010488220886 1104 70-71 0.2832096650544784 1104 72-73 0.32596744201822503 1104 74-75 0.3031607371196472 1104 76-77 0.09237190168865794 1104 78-79 0.2948862476126024 1104 80-81 0.31206265284210133 1104 82-83 0.24813254002373952 1104 84-85 0.3427493821904548 1104 86-87 0.3663349437295693 1104 88-89 0.34114861003561714 1104 90-91 0.31525678588020156 1104 92-93 0.33687465793506277 1104 94-95 0.2917133223466095 1104 96-97 0.3110896407837913 1104 98-99 0.3349541367867701 1104 100-101 0.6572437660032904 1105 1 0.13342343277781055 1105 2 0.17321955088034002 1105 3 0.13386882657437837 1105 4 0.08797012404743043 1105 5 0.10404638726826221 1105 6 0.12790336595413976 1105 7 0.13294382680072658 1105 8 0.12871531330883101 1105 9 0.20002489410305202 1105 10-11 0.19956968743930759 1105 12-13 0.2242465379125278 1105 14-15 0.2927933657162498 1105 16-17 0.24125282407961635 1105 18-19 0.15038571816532453 1105 20-21 0.1271891142004904 1105 22-23 0.08408617270924168 1105 24-25 0.09359360529381888 1105 26-27 0.18522543576367667 1105 28-29 0.1664199657461083 1105 30-31 0.19473282533463987 1105 32-33 0.152151888197956 1105 34-35 0.10593626025346481 1105 36-37 0.0841141333995381 1105 38-39 0.11275544174084473 1105 40-41 0.13218092389955416 1105 42-43 0.08255455203251216 1105 44-45 0.047159799877718456 1105 46-47 0.11991090908840363 1105 48-49 0.19707679462081984 1105 50-51 0.17256321448583378 1105 52-53 0.1679593360992655 1105 54-55 0.15292990664233486 1105 56-57 0.09056190634738925 1105 58-59 0.08917297659407808 1105 60-61 0.10878877243806073 1105 62-63 0.09857603454455699 1105 64-65 0.17164168142590341 1105 66-67 0.19211750937108008 1105 68-69 0.18110159642543522 1105 70-71 0.19695949594368045 1105 72-73 0.24844554673460095 1105 74-75 0.20090395227490632 1105 76-77 0.029567425413084436 1105 78-79 0.1283127378371418 1105 80-81 0.1336214518866612 1105 82-83 0.22160221575062167 1105 84-85 0.2135671675423083 1105 86-87 0.183634969776449 1105 88-89 0.21320234413192907 1105 90-91 0.11460106096561162 1105 92-93 0.1767169063934091 1105 94-95 0.1369665499542876 1105 96-97 0.14086685002472876 1105 98-99 0.1367979282122036 1105 100-101 0.37976363319515016 1106 1 0.034380957660793854 1106 2 0.05344124769641212 1106 3 0.05273967484695419 1106 4 0.0400542029914277 1106 5 0.0851925113513019 1106 6 0.10659241190464996 1106 7 0.11407140152843454 1106 8 0.03512547203648353 1106 9 0.13676185543204156 1106 10-11 0.10400874785641179 1106 12-13 0.1273840761964138 1106 14-15 0.17230016214671195 1106 16-17 0.1135683818517279 1106 18-19 0.041301143246144534 1106 20-21 0.0037297965530385113 1106 22-23 0.020709105608887057 1106 24-25 0.03799270625449225 1106 26-27 0.02144038434975215 1106 28-29 0.03769272438266569 1106 30-31 0.048282384553075985 1106 32-33 -0.012816485114278464 1106 34-35 -0.029222323677565498 1106 36-37 -0.04271939060249963 1106 38-39 -0.09102167742911149 1106 40-41 -0.02439861373279939 1106 42-43 -0.009216787105906121 1106 44-45 -0.08218620948205313 1106 46-47 0.011162138218494988 1106 48-49 0.018787091320717764 1106 50-51 0.01419721761895687 1106 52-53 0.03394322063859079 1106 54-55 0.08241472058262644 1106 56-57 0.06102959048122614 1106 58-59 -0.02788505560204868 1106 60-61 -0.015441462765679148 1106 62-63 -0.06557865397572016 1106 64-65 0.011033005489530012 1106 66-67 0.029861720441807904 1106 68-69 0.05061693975142134 1106 70-71 0.06896214892042707 1106 72-73 0.11509841226995832 1106 74-75 0.10403500077588035 1106 76-77 -0.11778458029201744 1106 78-79 0.0853295094828681 1106 80-81 0.05819631827791483 1106 82-83 0.04315056685255314 1106 84-85 0.07327202701663538 1106 86-87 0.09675180839344932 1106 88-89 0.07252869783739158 1106 90-91 -0.021330604397860498 1106 92-93 0.02842981644666054 1106 94-95 0.07810569445251758 1106 96-97 0.06908479245571186 1106 98-99 0.019760981120505505 1106 100-101 0.3569344907239511 1107 1 -0.048649160978598616 1107 2 -0.02312125944318666 1107 3 -0.020097913689944846 1107 4 -0.0101306168008648 1107 5 -0.04968684808067536 1107 6 -0.026254801246494708 1107 7 -0.0055074559267822565 1107 8 0.031305035127459746 1107 9 -0.03704692550743971 1107 10-11 0.045591745959093544 1107 12-13 0.013426991484777773 1107 14-15 0.04600588519204507 1107 16-17 -0.009273954084520142 1107 18-19 -0.07562685375978617 1107 20-21 -0.10790463206569711 1107 22-23 -0.11730905642118472 1107 24-25 -0.12488584159947891 1107 26-27 -0.09960055549498037 1107 28-29 -0.10320615546464751 1107 30-31 -0.17265654332246072 1107 32-33 -0.17118039265674412 1107 34-35 -0.2610905544749187 1107 36-37 -0.24493227651596072 1107 38-39 -0.3446697658683746 1107 40-41 -0.34948098006761796 1107 42-43 -0.20024737660502723 1107 44-45 -0.20509531945981507 1107 46-47 -0.16973913921061978 1107 48-49 -0.1936000006401457 1107 50-51 -0.06529852763915756 1107 52-53 -0.07309132190007261 1107 54-55 -0.07965012838666752 1107 56-57 -0.13234683961066196 1107 58-59 -0.10286668588747006 1107 60-61 -0.13295025279563788 1107 62-63 -0.14453541435216266 1107 64-65 -0.14584979459079506 1107 66-67 -0.055204358489042704 1107 68-69 -0.11348880718486498 1107 70-71 -0.10738866331582386 1107 72-73 -0.07804551933223536 1107 74-75 -0.1181740325285503 1107 76-77 -0.3659597467911553 1107 78-79 -0.15992782515831294 1107 80-81 -0.16891253901034275 1107 82-83 -0.23623774551703747 1107 84-85 -0.16987745654256514 1107 86-87 -0.06704471852195582 1107 88-89 -0.11858951545828234 1107 90-91 -0.1751735421024989 1107 92-93 -0.1394165159005638 1107 94-95 -0.1430876959970746 1107 96-97 -0.19266663768624426 1107 98-99 -0.2350068784349304 1107 100-101 0.08862171981334654 1108 1 -0.15705568881595866 1108 2 -0.11597527702797095 1108 3 -0.16847497078147455 1108 4 -0.1020825273827981 1108 5 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32-33 -0.5601007514196326 2308 34-35 -0.6789420769832972 2308 36-37 -0.9388293517777271 2308 38-39 -0.8367139507614283 2308 40-41 -0.7230289407969934 2308 42-43 -0.7284767228466151 2308 44-45 -0.6531399281802805 2308 46-47 -0.6224714630042882 2308 48-49 -0.5817767752359515 2308 50-51 -0.5071912313685232 2308 52-53 -0.5398035993833048 2308 54-55 -0.49206994023329287 2308 56-57 -0.6356669454274169 2308 58-59 -0.6086647810778274 2308 60-61 -0.7522611526456942 2308 62-63 -0.8930038167194851 2308 64-65 -0.7473506959624814 2308 66-67 -0.8434854674585068 2308 68-69 -0.8881145318035522 2308 70-71 -1.2380490715657615 2308 72-73 -1.0455544037150446 2308 74-75 -0.9536805341270167 2308 76-77 -1.2284744028896384 2308 78-79 -1.1117427803581066 2308 80-81 -1.1129069776892635 2308 82-83 -1.1574088084013923 2308 84-85 -1.1874802146445518 2308 86-87 -1.3130526116533119 2308 88-89 -1.2894990451018522 2308 90-91 -1.213993051969986 2308 92-93 -1.1173422120024732 2308 94-95 -1.15315216215388 2308 96-97 -1.1790093818952698 2308 98-99 -1.3366393228531592 2308 100-101 -1.2458417270984725 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 11.0 11 81.0 12 290.0 13 838.0 14 2298.0 15 4735.0 16 7814.0 17 11982.0 18 20108.0 19 33630.0 20 31273.0 21 28583.0 22 29218.0 23 32379.0 24 37789.0 25 46470.0 26 59109.0 27 75337.0 28 96234.0 29 121485.0 30 154251.0 31 200197.0 32 261890.0 33 355503.0 34 523527.0 35 942005.0 36 1532912.0 37 3218683.0 38 3167378.0 39 452102.0 40 267.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 26.423097279883496 30.82510827013842 21.554684450839083 21.197109999138995 2 24.947685532485984 28.654893625793537 24.412259317635556 21.98516152408492 3 25.199307528137677 25.911930919127208 25.522378373033533 23.366383179701582 4 25.986999682390717 25.880466050572203 25.156594655428865 22.97593961160822 5 25.645236877517085 27.250354974513023 23.54178741186783 23.562620736102055 6 25.461089294825058 26.098629334336326 22.657679309883086 25.78260206095553 7 25.552141486580766 26.167730820232276 23.99012995638946 24.289997736797496 8 24.8956205939723 26.120344198947294 23.822770018358057 25.161265188722354 9 24.69138207251874 25.544149088705048 24.249511655754933 25.51495718302128 10-11 25.037937685326455 26.105647795203147 24.20013785357735 24.65627666589305 12-13 24.873082368931122 25.888764077331533 24.456918321123446 24.781235232613902 14-15 24.636928948631066 25.929334624578726 24.564198127962044 24.869538298828157 16-17 24.79913919010689 26.238135944949327 24.3816401027979 24.58108476214589 18-19 24.608566854748606 25.998872853528 24.728885198507143 24.663675093216252 20-21 24.347892395945312 26.041359217754756 24.911072563198687 24.699675823101245 22-23 24.2250234727554 26.046997570573094 25.28036501936213 24.447613937309377 24-25 24.265688618449825 25.723724729937747 25.632087302490596 24.378499349121828 26-27 24.168246875824078 25.737923246601113 25.847685510760954 24.24614436681385 28-29 24.283551409330524 25.902121164926495 25.796210974496915 24.018116451246065 30-31 24.268544827881687 25.79871152729686 25.786731645985466 24.146011998835988 32-33 24.15395664311952 25.844095482862684 25.872025201122362 24.129922672895436 34-35 24.224630404007414 25.99522168160226 25.766066968956913 24.01408094543341 36-37 24.313075239734815 25.892587064072565 25.647491229981117 24.146846466211507 38-39 24.17092847817145 25.96552752140718 25.69348027349549 24.170063726925882 40-41 24.325146585924685 26.184196728611564 25.42689780801453 24.063758877449217 42-43 24.37186893117595 26.080977313183617 25.359660465112622 24.18749329052781 44-45 24.209081372119982 26.25831675877665 25.386110739875985 24.146491129227385 46-47 24.29410832898518 26.373968906145734 25.253020317924708 24.07890244694438 48-49 24.3557231988913 26.282043947007693 25.206498666754477 24.15573418734652 50-51 24.24927581450614 26.283603119708037 25.330262913203693 24.136858152582125 52-53 24.245987139314657 26.431279048326406 25.26571665735385 24.057017155005088 54-55 24.3222999601035 26.330266594662255 25.212439584753206 24.134993860481043 56-57 24.149289672052994 26.408780845209982 25.345900649297448 24.09602883343958 58-59 24.21872178228334 26.52002745718266 25.234979947582932 24.02627081295107 60-61 24.26998180266394 26.39778522356746 25.14270360895634 24.18952936481226 62-63 24.23391119389042 26.469214549937593 25.216508817536525 24.08036543863546 64-65 24.210217883247925 26.636832166370457 25.163719684681997 23.989230265699625 66-67 24.227582787047844 26.477809653226892 25.164732928565698 24.129874631159574 68-69 24.16338581172635 26.606783284509028 25.180701606889194 24.049129296875428 70-71 24.222549255449113 26.73473919187504 25.095559388412923 23.94715216426292 72-73 24.258698374081316 26.66095483873673 25.0656742289207 24.014672558261257 74-75 24.12217787202195 26.71089079263987 25.1650990943964 24.00183224094178 76-77 24.17497333889921 26.8646370775398 25.05099216680306 23.909397416757923 78-79 24.20520500077828 26.72920463810428 25.065232403978303 24.000357957139133 80-81 24.0349632149708 26.834743110567523 25.136184030970448 23.994109643491228 82-83 24.132182424621202 26.98394833489033 25.010815973837264 23.873053266651205 84-85 24.13691809166845 26.842201001845023 25.008312313251373 24.012568593235155 86-87 24.002408902825472 26.9799562216982 25.08290375599905 23.934731119477274 88-89 24.067723237025696 27.124016030110244 24.989202574122054 23.81905815874201 90-91 24.169542156706843 26.968476692054484 24.92338080324931 23.93860034798936 92-93 24.078768043894737 27.041850545899948 24.966773443824287 23.912607966381028 94-95 24.100872305320443 27.231851925906525 24.898806546838735 23.768469221934296 96-97 24.021609370139863 27.202076069014907 24.839066567310432 23.9372479935348 98-99 24.05128156302175 27.196520946454346 24.903365447019755 23.848832043504153 100-101 24.073295203314967 27.329484518000125 24.759883607883094 23.837336670801815 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 176594.0 1 99785.5 2 16106.0 3 7931.5 4 5851.5 5 4446.5 6 3593.0 7 3289.5 8 3161.0 9 3027.5 10 3004.5 11 3427.0 12 4668.5 13 7560.5 14 9910.0 15 9997.0 16 24991.0 17 23856.5 18 6505.0 19 5520.5 20 5730.5 21 6585.0 22 8160.0 23 10337.0 24 12883.0 25 16101.5 26 20414.0 27 25714.0 28 31740.5 29 39089.0 30 48503.0 31 57463.5 32 66719.5 33 79039.0 34 92365.5 35 107244.0 36 128350.5 37 151088.0 38 174120.0 39 199617.5 40 223491.0 41 244107.0 42 265517.0 43 291422.0 44 320328.5 45 351923.0 46 389717.0 47 431049.0 48 468953.0 49 499527.5 50 513275.5 51 520651.0 52 534220.0 53 545952.5 54 542446.0 55 522873.5 56 489891.5 57 448048.0 58 405258.0 59 363585.0 60 319907.0 61 271454.5 62 221958.0 63 172958.5 64 127502.0 65 89633.0 66 63326.5 67 47676.0 68 36203.5 69 27129.0 70 20938.0 71 15696.5 72 12228.0 73 9347.0 74 6959.0 75 5689.0 76 4470.5 77 2874.0 78 1686.0 79 931.0 80 459.5 81 284.5 82 214.0 83 164.5 84 114.0 85 75.0 86 39.5 87 8.5 88 5.5 89 4.0 90 3.0 91 3.5 92 3.0 93 1.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07201019463104777 2 0.0026466629031061954 3 0.0010132438836974213 4 0.003065936234291335 5 0.003266838038817548 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 7.861374959721372E-5 14-15 0.0 16-17 7.905049265053158E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 1.6159492972760598E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 5.197242334482462E-4 42-43 1.0481833279628497E-4 44-45 9.520998562329218E-4 46-47 3.4502701212110466E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 5.677659693132102E-5 56-57 4.36743053317854E-5 58-59 7.424631906403518E-5 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.4412520759489182E-4 70-71 8.73486106635708E-6 72-73 0.0017906465186032013 74-75 8.996906898347792E-4 76-77 4.542127754505682E-4 78-79 7.075237463749235E-4 80-81 0.0015329681171456675 82-83 2.9698527625614073E-4 84-85 1.3102291599535621E-5 86-87 1.1792062439582058E-4 88-89 4.2364076171831833E-4 90-91 3.5376187318746175E-4 92-93 7.11891176908102E-4 94-95 0.003323614635748869 96-97 1.0481833279628497E-4 98-99 8.123420791712084E-4 100-101 0.0015810098530106315 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.1448379E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.36607407235859 #Duplication Level Percentage of deduplicated Percentage of total 1 73.67126080442286 34.15847135461919 2 14.196561603068266 13.164776537213301 3 4.667696873607318 6.492683369669805 4 2.0360901136414373 3.776220201083836 5 1.0902086507941318 2.5274347528523418 6 0.6608776043653913 1.838537997406118 7 0.45337161964461414 1.4714743469123155 8 0.33678569331846203 1.2492344322331563 9 0.27123588691590805 1.1318528901442384 >10 2.3627449364531596 23.080975631213647 >50 0.23170703538694035 7.050073406673666 >100 0.020637593854939382 1.2655758125455843 >500 3.740088619743154E-4 0.11885266567703727 >1k 3.1703351765896386E-4 0.34753156112049605 >5k 5.59466344351842E-5 0.15167268831088412 >10k+ 7.459551258024561E-5 2.174632352324407 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 185687 1.621950146828647 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28795 0.2515203244057521 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18546 0.1619967333366584 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 18469 0.16132414903454892 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0039219526187943285 0.0 0.0 0.0 0.0 2 0.003930687479860686 8.73486106635708E-6 0.0 0.0 8.73486106635708E-6 3 0.0039481572019934 8.73486106635708E-6 0.0 0.0 8.73486106635708E-6 4 0.003983096646258829 8.73486106635708E-6 0.0 0.0 2.620458319907124E-5 5 0.004035505812656971 8.73486106635708E-6 0.0 0.0 5.240916639814248E-5 6 0.004070445256922399 8.73486106635708E-6 0.0 8.73486106635708E-6 7.861374959721372E-5 7 0.004122854423320541 8.73486106635708E-6 0.0 8.73486106635708E-6 8.73486106635708E-5 8 0.0042014681729177555 1.746972213271416E-5 0.0 1.746972213271416E-5 9.608347172992787E-5 9 0.004236407617183184 1.746972213271416E-5 0.0 1.746972213271416E-5 1.0481833279628495E-4 10-11 0.004341225949979469 4.36743053317854E-5 0.0 1.746972213271416E-5 1.3102291599535618E-4 12-13 0.004454779143842111 4.36743053317854E-5 0.0 1.746972213271416E-5 1.4849263812807036E-4 14-15 0.004773601572764144 4.36743053317854E-5 0.0 2.620458319907124E-5 1.7032979079396307E-4 16-17 0.005166670320750213 4.36743053317854E-5 0.0 2.620458319907124E-5 2.052692350593914E-4 18-19 0.005350102403143712 5.240916639814248E-5 0.0 3.057201373224978E-5 2.3584124879164116E-4 20-21 0.00556410649926946 5.240916639814248E-5 0.0 3.493944426542832E-5 2.838829846566051E-4 22-23 0.005765008303795673 5.240916639814248E-5 0.0 3.493944426542832E-5 3.1882242892203343E-4 24-25 0.005965910108321886 6.551145799767809E-5 0.0 3.493944426542832E-5 3.6686416478699736E-4 26-27 0.006267262815111205 6.987888853085664E-5 0.0 3.493944426542832E-5 4.105384701187828E-4 28-29 0.006568615521900524 8.73486106635708E-5 0.0 3.493944426542832E-5 4.673150670501038E-4 30-31 0.006961684269886593 9.608347172992787E-5 0.0 3.493944426542832E-5 5.066219418487106E-4 32-33 0.007354753017872662 9.608347172992787E-5 0.0 4.36743053317854E-5 5.284590945146034E-4 34-35 0.007804598362790051 9.608347172992787E-5 0.0 4.36743053317854E-5 5.677659693132102E-4 36-37 0.008132155652778441 9.608347172992787E-5 0.0 4.36743053317854E-5 6.114402746449955E-4 38-39 0.008621307872494438 1.091857633294635E-4 0.0 4.804173586496394E-5 6.463797189104239E-4 40-41 0.009403077937933397 1.1792062439582057E-4 0.0 5.240916639814248E-5 6.813191631758523E-4 42-43 0.009966476476713428 1.3975777706171327E-4 0.0 5.240916639814248E-5 6.987888853085664E-4 44-45 0.010542977307092995 1.4412520759489182E-4 0.0 5.240916639814248E-5 7.511980517067088E-4 46-47 0.011460137719060489 1.5722749919442743E-4 0.0 5.240916639814248E-5 8.25444370770744E-4 48-49 0.01250395361649016 1.6159492972760598E-4 0.0 5.240916639814248E-5 8.691186761025294E-4 50-51 0.013473523194855796 1.7906465186032014E-4 0.0 5.240916639814248E-5 8.953232593016008E-4 52-53 0.01465709686934718 1.8343208239349868E-4 0.0 5.240916639814248E-5 9.171604119674934E-4 54-55 0.015604829295046923 1.877995129266772E-4 0.0 5.240916639814248E-5 9.520998562329217E-4 56-57 0.016670482345142488 1.965343739930343E-4 0.0 5.240916639814248E-5 0.0010001415920978857 58-59 0.01752649872964548 2.096366655925699E-4 0.0 6.114402746449956E-5 0.0010394484668964925 60-61 0.01850917059961065 2.096366655925699E-4 0.0 6.114402746449956E-5 0.0010612856195623853 62-63 0.019998464411424534 2.096366655925699E-4 8.73486106635708E-6 6.114402746449956E-5 0.0010787553416950996 64-65 0.022129770511615663 2.18371526658927E-4 8.73486106635708E-6 6.114402746449956E-5 0.0011049599248941708 66-67 0.02500790723298032 2.402086793248197E-4 8.73486106635708E-6 6.114402746449956E-5 0.0011704713828918486 68-69 0.028685283741916652 2.533109709243553E-4 8.73486106635708E-6 6.114402746449956E-5 0.0012316154103563484 70-71 0.03470360301663668 2.620458319907124E-4 8.73486106635708E-6 6.114402746449956E-5 0.0012883920072876693 72-73 0.04191859825744763 2.7951555412342654E-4 8.73486106635708E-6 7.424631906403518E-5 0.0013451686042189903 74-75 0.04971446175917132 3.0572013732249777E-4 8.73486106635708E-6 7.861374959721372E-5 0.001367005756884883 76-77 0.059912412054143216 3.2755728998839053E-4 8.73486106635708E-6 7.861374959721372E-5 0.0013975777706171327 78-79 0.0734863861512621 3.3192472052156905E-4 8.73486106635708E-6 7.861374959721372E-5 0.001432517214882561 80-81 0.08992976210867931 3.3192472052156905E-4 8.73486106635708E-6 7.861374959721372E-5 0.0014892938118138822 82-83 0.11119041394419245 3.4502701212110466E-4 8.73486106635708E-6 8.73486106635708E-5 0.00155480526981156 84-85 0.1372901788104674 3.5812930372064027E-4 8.73486106635708E-6 9.171604119674934E-5 0.0016246841583424168 86-87 0.1700371729482401 3.5812930372064027E-4 8.73486106635708E-6 1.0045090226310641E-4 0.0016639910331410239 88-89 0.2098419348276293 3.6686416478699736E-4 8.73486106635708E-6 1.0481833279628495E-4 0.0017032979079396305 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 4300 0.0 84.57471 1 TCGACGG 4870 0.0 74.61906 2 TATCTCG 4815 0.0 51.695446 2 CGACGGC 7125 0.0 51.40146 3 ACGGCGC 7570 0.0 48.189907 5 ATATCTC 5300 0.0 47.449024 1 GACGGCG 8190 0.0 44.71719 4 CGGCGCG 8945 0.0 40.781227 6 ATCTCGA 8430 0.0 36.03469 1 AATTCGA 4245 0.0 28.220863 1 TCTCGAG 10825 0.0 27.909145 2 TCGAGGG 12640 0.0 24.31427 4 CTCGAGG 12745 0.0 23.778625 3 CGCGCCG 21445 0.0 22.126907 9 CGAGGGC 14235 0.0 21.622618 5 GGGCGCG 14325 0.0 21.320513 8 AATTCCC 6560 0.0 20.870659 1 AGGGCGC 14985 0.0 20.666748 7 ATTCGAT 945 0.0 20.60959 2 GGCGCGC 23750 0.0 20.09943 7 >>END_MODULE