FastQCFastQC Report
Tue 31 May 2016
SRR1051266_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051266_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12232916
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT10536688.61338375903178No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT1047550.8563371153697122No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA752020.6147512171259902No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT325810.2663387862714009No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228210.18655404811085108No Hit
GTCGACGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175490.14345721003888198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG901650.094.230911
ATATCTC1337050.092.735711
TATCTCG1338250.092.5354462
TCGACGG928400.091.5004962
ATCTCGA1389650.089.0478443
ACGGCGC953050.088.9844065
GACGGCG956350.088.722044
CGACGGC957650.088.586733
CGGCGCG961650.088.2626956
TCTCGAG1389950.087.750734
TCGAGGG1409750.086.494686
CTCGAGG1411000.086.3877645
CGAGGGC1425250.085.467397
AGGGCGC1436700.084.958149
GAGGGCG1449800.084.180658
CTCGACG34750.049.0637445
TCTCGAC37900.045.3618164
AATTCGA46250.044.6682171
GGGCGCG1443450.042.3972310-11
GGATCCT1427900.041.2491518-19
Produced by FastQC (version 0.11.5)