##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051266_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12232916 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08445059215644 33.0 31.0 34.0 30.0 34.0 2 32.31841753838578 34.0 31.0 34.0 30.0 34.0 3 32.39866120228407 34.0 31.0 34.0 30.0 34.0 4 35.81895085358225 37.0 35.0 37.0 35.0 37.0 5 35.80799034343079 37.0 35.0 37.0 35.0 37.0 6 35.81134293736669 37.0 35.0 37.0 35.0 37.0 7 35.75987491453387 37.0 35.0 37.0 35.0 37.0 8 35.74749356572055 37.0 35.0 37.0 33.0 37.0 9 37.42588807116799 39.0 37.0 39.0 34.0 39.0 10-11 37.40218125424878 39.0 37.0 39.0 34.0 39.0 12-13 37.36502772519651 39.0 37.0 39.0 34.0 39.0 14-15 38.72155130469301 40.0 38.0 41.0 34.0 41.0 16-17 38.57628336530718 40.0 38.0 41.0 33.5 41.0 18-19 38.5713190542631 40.0 38.0 41.0 34.0 41.0 20-21 38.536345054605135 40.0 38.0 41.0 34.0 41.0 22-23 38.52214292160593 40.0 38.0 41.0 34.0 41.0 24-25 38.536596916058286 40.0 38.0 41.0 34.0 41.0 26-27 38.479083523503306 40.0 38.0 41.0 34.0 41.0 28-29 38.26368336870784 40.0 38.0 41.0 34.0 41.0 30-31 37.66846960283223 39.5 36.5 41.0 33.0 41.0 32-33 37.09825277963161 38.5 35.0 40.5 33.0 41.0 34-35 36.808981685151764 38.0 35.0 40.0 32.0 41.0 36-37 36.912071373661036 38.0 35.0 41.0 33.0 41.0 38-39 36.71965220720881 39.0 35.0 41.0 31.5 41.0 40-41 35.90166800785684 39.0 35.0 41.0 27.5 41.0 42-43 35.899261835853366 39.0 35.0 41.0 27.0 41.0 44-45 35.81147692013907 38.0 35.0 41.0 27.0 41.0 46-47 35.842865838365924 38.0 35.0 41.0 27.5 41.0 48-49 35.66238421812101 38.0 35.0 41.0 26.0 41.0 50-51 35.45648376069941 38.0 34.5 40.5 27.0 41.0 52-53 35.309515736068164 38.0 34.0 40.0 27.0 41.0 54-55 35.06623845859809 38.0 34.5 40.0 26.5 41.0 56-57 34.698873637324084 37.0 34.0 40.0 25.5 41.0 58-59 34.40386400920271 37.0 34.0 40.0 25.0 41.0 60-61 34.23633441119027 36.5 34.0 40.0 25.0 41.0 62-63 33.93241202669911 36.0 34.0 39.5 25.0 41.0 64-65 33.59190654133487 35.5 34.0 39.0 24.5 41.0 66-67 33.14549078077541 35.0 33.5 39.0 22.5 41.0 68-69 32.71456122971824 35.0 33.0 38.5 21.5 40.0 70-71 32.26126681487881 35.0 33.0 37.0 19.5 39.5 72-73 31.89632128594687 35.0 33.0 37.0 19.0 39.0 74-75 31.53930399750967 35.0 32.0 36.0 18.0 39.0 76-77 31.122050989314403 35.0 32.0 36.0 14.5 38.0 78-79 30.839138558623308 35.0 32.0 35.0 11.0 37.0 80-81 30.5841054986399 35.0 32.0 35.0 7.5 37.0 82-83 30.31727222683455 35.0 31.0 35.0 7.0 36.0 84-85 30.02033284623225 35.0 31.0 35.0 2.0 36.0 86-87 29.75540978945658 35.0 31.0 35.0 2.0 36.0 88-89 29.489764337464592 35.0 31.0 35.0 2.0 35.0 90-91 29.28455754130904 35.0 31.0 35.0 2.0 35.0 92-93 29.089109742926382 34.5 30.0 35.0 2.0 35.0 94-95 28.799458444740402 34.0 30.0 35.0 2.0 35.0 96-97 28.486801061987183 34.0 30.0 35.0 2.0 35.0 98-99 28.154307525695426 34.0 29.0 35.0 2.0 35.0 100-101 27.024196193287032 33.5 27.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34972782351974985 1101 2 0.3448747959423173 1101 3 0.3425445174323869 1101 4 0.33457485363037875 1101 5 0.34852010680488377 1101 6 0.32066751944518757 1101 7 0.36304580400704367 1101 8 0.2905785718756917 1101 9 0.38198775772833926 1101 10-11 0.32584061536538655 1101 12-13 0.3585132593038054 1101 14-15 0.5195162664276651 1101 16-17 0.37092030548968324 1101 18-19 0.46532317529086953 1101 20-21 0.4396428092735434 1101 22-23 0.4302310060946084 1101 24-25 0.4843435571596473 1101 26-27 0.5269944827757627 1101 28-29 0.617336892770254 1101 30-31 0.6858331676214533 1101 32-33 0.6512680364838062 1101 34-35 0.6997134498942188 1101 36-37 0.7367183526707706 1101 38-39 0.7285949688682862 1101 40-41 0.7198363153802774 1101 42-43 0.6438337021137244 1101 44-45 0.6698490060185094 1101 46-47 0.728502969470874 1101 48-49 0.6942398179095548 1101 50-51 0.6643581426305971 1101 52-53 0.5656126123446157 1101 54-55 0.5849928686124386 1101 56-57 0.578168599619886 1101 58-59 0.5775500552996107 1101 60-61 0.5170546814302028 1101 62-63 0.5638110066568416 1101 64-65 0.5193919983733153 1101 66-67 0.47355575881809386 1101 68-69 0.42474102299950545 1101 70-71 0.5202950907953436 1101 72-73 0.5429884799223963 1101 74-75 0.49383004888165516 1101 76-77 0.361026978424853 1101 78-79 0.30271675910305973 1101 80-81 0.32255297944249506 1101 82-83 0.3744955460997552 1101 84-85 0.3706353895850292 1101 86-87 0.36164481157626227 1101 88-89 0.4195063838647748 1101 90-91 0.4828829191833286 1101 92-93 0.45642966353947045 1101 94-95 0.45647472352424145 1101 96-97 0.430799628737109 1101 98-99 0.4857345327709055 1101 100-101 0.6636520471356171 1102 1 0.33914069114747747 1102 2 0.3198448715374269 1102 3 0.36140749086750645 1102 4 0.2677296730386942 1102 5 0.2975649097186377 1102 6 0.30180773462637944 1102 7 0.32380057256755634 1102 8 0.2874057012549258 1102 9 0.38334466580788273 1102 10-11 0.3668411724925775 1102 12-13 0.3636002638884861 1102 14-15 0.5331064604182245 1102 16-17 0.484379541489929 1102 18-19 0.5370796145648526 1102 20-21 0.4544833204555019 1102 22-23 0.4715198672799872 1102 24-25 0.4908034204973717 1102 26-27 0.5155470617225646 1102 28-29 0.5433399722447803 1102 30-31 0.5820126935829251 1102 32-33 0.5697341000707681 1102 34-35 0.6477346310524581 1102 36-37 0.6744262124945877 1102 38-39 0.6665129129553762 1102 40-41 0.6052843861216459 1102 42-43 0.6321001862579152 1102 44-45 0.6178396599715228 1102 46-47 0.5942540614049392 1102 48-49 0.6030199356053316 1102 50-51 0.5641415110751495 1102 52-53 0.5149536073459586 1102 54-55 0.47247206516858853 1102 56-57 0.47008577309433974 1102 58-59 0.4480809058608912 1102 60-61 0.36074918811845436 1102 62-63 0.4302429440929103 1102 64-65 0.3390288580976417 1102 66-67 0.3059374647970543 1102 68-69 0.36884343267025343 1102 70-71 0.46644579925729346 1102 72-73 0.4330124883811486 1102 74-75 0.4080014107099217 1102 76-77 0.31051471051208424 1102 78-79 0.24060583102864896 1102 80-81 0.3060414511065055 1102 82-83 0.34495877825616716 1102 84-85 0.27633829514939023 1102 86-87 0.2774134500103749 1102 88-89 0.39215361321229025 1102 90-91 0.4571924956852804 1102 92-93 0.41358372508960173 1102 94-95 0.44589240444420497 1102 96-97 0.4316665072250778 1102 98-99 0.455526280291771 1102 100-101 0.5183702988725649 1103 1 0.28828568234268914 1103 2 0.2606511632642565 1103 3 0.3052768866795006 1103 4 0.26537147010854056 1103 5 0.2779613872722848 1103 6 0.25597934750585694 1103 7 0.3152107374243158 1103 8 0.2866669984382426 1103 9 0.34465316901431464 1103 10-11 0.324871801482324 1103 12-13 0.35576775781582626 1103 14-15 0.4397837035818668 1103 16-17 0.5081588441628924 1103 18-19 0.4885435674417593 1103 20-21 0.44692404101656535 1103 22-23 0.4628444123476001 1103 24-25 0.45782784151190725 1103 26-27 0.4655756774138311 1103 28-29 0.4461889243018078 1103 30-31 0.4780941690860203 1103 32-33 0.4451689344029859 1103 34-35 0.47234090613761026 1103 36-37 0.42182775306317666 1103 38-39 0.3660762464871752 1103 40-41 0.41287098406630207 1103 42-43 0.40120583752987216 1103 44-45 0.3890966782164895 1103 46-47 0.41278232929526126 1103 48-49 0.44136596943836537 1103 50-51 0.39057191867856744 1103 52-53 0.40220623156893964 1103 54-55 0.3951696190425338 1103 56-57 0.38633254523659843 1103 58-59 0.3811354399290252 1103 60-61 0.33425153336339264 1103 62-63 0.44535893908909685 1103 64-65 0.42872020222245055 1103 66-67 0.38556655039101884 1103 68-69 0.3498167489539128 1103 70-71 0.437118360578971 1103 72-73 0.4472952287621048 1103 74-75 0.38253652214093137 1103 76-77 0.3935419371615545 1103 78-79 0.2829261073579943 1103 80-81 0.31561673149538194 1103 82-83 0.4222612659586851 1103 84-85 0.3754502275156888 1103 86-87 0.42258993764658825 1103 88-89 0.4859195473904343 1103 90-91 0.46699866401317536 1103 92-93 0.45362453737388364 1103 94-95 0.5049204928905304 1103 96-97 0.4993944005503046 1103 98-99 0.5474185851764943 1103 100-101 0.6519465800899695 1104 1 0.2632073927516956 1104 2 0.25670301822356834 1104 3 0.20794989595471947 1104 4 0.17146943822029215 1104 5 0.16880222881132312 1104 6 0.20237194813427095 1104 7 0.24110637947772062 1104 8 0.2120847902029368 1104 9 0.2368990573768599 1104 10-11 0.2338960595432127 1104 12-13 0.309980343046405 1104 14-15 0.36191368057797035 1104 16-17 0.4172184564878023 1104 18-19 0.36523401688317136 1104 20-21 0.39850140219615326 1104 22-23 0.4208801324059337 1104 24-25 0.39564098538821213 1104 26-27 0.40914070755955123 1104 28-29 0.39206274960884 1104 30-31 0.4541086953396132 1104 32-33 0.4386038860970629 1104 34-35 0.5168856127228949 1104 36-37 0.47163282922367245 1104 38-39 0.41725655979328025 1104 40-41 0.2920813532079336 1104 42-43 0.2758685472559179 1104 44-45 0.3066907334231388 1104 46-47 0.34557220294593805 1104 48-49 0.31552759188442536 1104 50-51 0.2288105202138837 1104 52-53 0.23069530372991665 1104 54-55 0.2741746063458237 1104 56-57 0.2636777533087127 1104 58-59 0.30816910457534163 1104 60-61 0.19304801759204082 1104 62-63 0.36004786069986494 1104 64-65 0.3260491215520531 1104 66-67 0.4209325537837927 1104 68-69 0.4051359312885907 1104 70-71 0.4272282685716249 1104 72-73 0.42541493042262957 1104 74-75 0.4272910179563283 1104 76-77 0.4855731639005647 1104 78-79 0.3473092540769507 1104 80-81 0.37013548261014506 1104 82-83 0.37089558037288484 1104 84-85 0.3732816624693065 1104 86-87 0.37284819963323557 1104 88-89 0.4259112554195603 1104 90-91 0.5081546588500636 1104 92-93 0.37780764066196326 1104 94-95 0.4774717605094665 1104 96-97 0.5388389109048894 1104 98-99 0.5518986736033327 1104 100-101 0.6009944038550152 1105 1 0.05535489596954335 1105 2 0.07826633377084136 1105 3 0.10741948130657875 1105 4 -0.05045803171119445 1105 5 0.07433057193706816 1105 6 0.0775407658249847 1105 7 0.08704674123789857 1105 8 0.10734030582609932 1105 9 0.10984636867053155 1105 10-11 0.0280699294752651 1105 12-13 0.10557248921362827 1105 14-15 0.19210906314980036 1105 16-17 0.06366568291406338 1105 18-19 0.1544859323501342 1105 20-21 0.07570916732587563 1105 22-23 0.049384420543624685 1105 24-25 0.0654446787735381 1105 26-27 0.14792295424386026 1105 28-29 0.22558969045140032 1105 30-31 0.21908934775432698 1105 32-33 0.13910538594416977 1105 34-35 0.20916606374949964 1105 36-37 0.216458707811384 1105 38-39 0.2397718898258958 1105 40-41 0.19065409811957323 1105 42-43 0.18009735523096992 1105 44-45 0.1019403076877694 1105 46-47 0.11151334102846278 1105 48-49 0.1429407841369823 1105 50-51 0.16716932434582077 1105 52-53 0.01348568852426979 1105 54-55 0.097059086754264 1105 56-57 0.1546132020120936 1105 58-59 0.1428475417976358 1105 60-61 0.05681554849923742 1105 62-63 0.13912559142205794 1105 64-65 0.04229702535099733 1105 66-67 0.03691902153812521 1105 68-69 0.12586066232108095 1105 70-71 0.11591404526243565 1105 72-73 0.18015224414805786 1105 74-75 0.17059687924330547 1105 76-77 0.25669047326990224 1105 78-79 0.21309711382868102 1105 80-81 0.16393569647030404 1105 82-83 0.22502812685641516 1105 84-85 0.1672530975187847 1105 86-87 0.20972536204255476 1105 88-89 0.2284733208853531 1105 90-91 0.3744659201596825 1105 92-93 0.31040379694291786 1105 94-95 0.39470104803432093 1105 96-97 0.2796162008306986 1105 98-99 0.2954249649148579 1105 100-101 0.37129833912554133 1106 1 -0.1263528574310051 1106 2 -0.17165971885174258 1106 3 -0.15367040135618026 1106 4 -0.2900327624416619 1106 5 -0.12489859171678575 1106 6 -0.08804245494428642 1106 7 -0.08707215997211648 1106 8 -0.1017299357623358 1106 9 -0.11042611274910996 1106 10-11 -0.12115327593685521 1106 12-13 -0.12637104013682432 1106 14-15 -0.26006702217131306 1106 16-17 -0.20219801121463377 1106 18-19 -0.25409867379218554 1106 20-21 -0.36608116114150135 1106 22-23 -0.18176054536818498 1106 24-25 -0.08289189297408939 1106 26-27 -0.37339456966848417 1106 28-29 -0.23873504237245413 1106 30-31 -0.19200830327882557 1106 32-33 -0.26909007534364804 1106 34-35 -0.19948100849711636 1106 36-37 -0.3785261045150108 1106 38-39 -0.23690660692081167 1106 40-41 -0.25220414917138356 1106 42-43 -0.27740024326147505 1106 44-45 -0.27044321869220767 1106 46-47 -0.1486568095429348 1106 48-49 -0.10912811162198466 1106 50-51 -0.2990931614394512 1106 52-53 -0.2920998676180204 1106 54-55 -0.21404133983573814 1106 56-57 -0.11047267941336258 1106 58-59 -0.09752777390490053 1106 60-61 -0.11839870625579607 1106 62-63 -0.009880224677203842 1106 64-65 -0.07585535055748238 1106 66-67 -0.12473545631435456 1106 68-69 0.015814136752474894 1106 70-71 0.05675496754877685 1106 72-73 -0.04910970177035168 1106 74-75 0.04954757049494418 1106 76-77 0.13846832985373325 1106 78-79 -0.08663504998744642 1106 80-81 -0.1309328049199685 1106 82-83 -0.0016081220727350853 1106 84-85 -0.050255422818079154 1106 86-87 5.549492413230439E-4 1106 88-89 0.05668032345584706 1106 90-91 0.07734126937399921 1106 92-93 -0.06089533390229818 1106 94-95 -0.004798849269022298 1106 96-97 -0.10784416912597905 1106 98-99 -0.017273950020097573 1106 100-101 0.030269002051277027 1107 1 -0.25467432966802406 1107 2 -0.3430031646974925 1107 3 -0.3257822621796933 1107 4 -0.22237895281003972 1107 5 -0.30790928005589535 1107 6 -0.21521798933607528 1107 7 -0.25045853779921146 1107 8 -0.22577524649122438 1107 9 -0.2292898732079891 1107 10-11 -0.27715425509726543 1107 12-13 -0.22107838642634192 1107 14-15 -0.2959572097641825 1107 16-17 -0.32392337997134035 1107 18-19 -0.35528017050835814 1107 20-21 -0.33143614778463615 1107 22-23 -0.28674377323208233 1107 24-25 -0.3658049786340314 1107 26-27 -0.3001799525370856 1107 28-29 -0.3204710180277104 1107 30-31 -0.1908669283438087 1107 32-33 -0.2875252432922153 1107 34-35 -0.45049143127221214 1107 36-37 -0.3504665272294005 1107 38-39 -0.3186668594443418 1107 40-41 -0.3409974551738415 1107 42-43 -0.34344351434187104 1107 44-45 -0.34624151316501184 1107 46-47 -0.30698843471758863 1107 48-49 -0.26795902294680474 1107 50-51 -0.28589839317164234 1107 52-53 -0.19698791886811762 1107 54-55 -0.1863121970009871 1107 56-57 -0.1322214780353832 1107 58-59 -0.09364791838380881 1107 60-61 -0.12206012641328101 1107 62-63 -0.10641691961910738 1107 64-65 -0.21740073411397987 1107 66-67 -0.11347769747830228 1107 68-69 -0.05644556620521968 1107 70-71 0.061281293401556525 1107 72-73 0.09853805412014438 1107 74-75 0.03584949905848944 1107 76-77 -0.33100853572189237 1107 78-79 -0.32598706182452375 1107 80-81 -0.16491158234055803 1107 82-83 -0.05716073230851748 1107 84-85 -0.15846921993481544 1107 86-87 -0.19942990499557922 1107 88-89 -0.11767882889730785 1107 90-91 -0.04009441068947339 1107 92-93 -0.16518001612577393 1107 94-95 -0.10530251960841497 1107 96-97 -0.19485580102063338 1107 98-99 -0.21982602977883303 1107 100-101 -0.2651547108115082 1108 1 -0.4759146742893279 1108 2 -0.3733873763775044 1108 3 -0.3724238292798958 1108 4 -0.48354244920733436 1108 5 -0.7206797493036916 1108 6 -0.44192692393978916 1108 7 -0.4629143413625272 1108 8 -1.2164580305810446 1108 9 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0.1490520464515086 2305 10-11 0.10315016746160666 2305 12-13 0.11250354447891198 2305 14-15 0.22052923817112458 2305 16-17 0.20924246626371712 2305 18-19 0.14247342259537277 2305 20-21 0.18489096741226518 2305 22-23 0.17132816406086704 2305 24-25 0.1653139557179557 2305 26-27 0.12163614471178619 2305 28-29 0.12299909662461062 2305 30-31 0.050488993259961035 2305 32-33 0.1473330185914321 2305 34-35 0.08470331579205492 2305 36-37 -0.017033452582019493 2305 38-39 0.09816091501313196 2305 40-41 0.028196010232697688 2305 42-43 0.0736090154560074 2305 44-45 0.08353435389970798 2305 46-47 0.054815651163430346 2305 48-49 0.07108975179383492 2305 50-51 0.10004727112674061 2305 52-53 0.08645169193537328 2305 54-55 0.03855426310354204 2305 56-57 0.12804028705490111 2305 58-59 0.0877459193479524 2305 60-61 0.17090948821567054 2305 62-63 0.16843941883844593 2305 64-65 0.07757904045318043 2305 66-67 0.1553942377742885 2305 68-69 0.0744557861766495 2305 70-71 0.06454319968745637 2305 72-73 0.021660269257314013 2305 74-75 0.14338134651097434 2305 76-77 0.1965966666804455 2305 78-79 0.15872402030353427 2305 80-81 0.12295407675310344 2305 82-83 0.07646104558189037 2305 84-85 0.13051855448614091 2305 86-87 0.12992938455981573 2305 88-89 0.1315604671505035 2305 90-91 0.175840285138527 2305 92-93 0.18057544050962804 2305 94-95 0.29891355612645043 2305 96-97 0.33993570305711884 2305 98-99 0.28185951584633173 2305 100-101 0.377635054427909 2306 1 -0.27919436790134355 2306 2 -0.48414980012771025 2306 3 -0.33458183521582896 2306 4 -0.23083943237493543 2306 5 -0.24555089422879917 2306 6 -0.23769646902530894 2306 7 -0.13405824645453635 2306 8 -0.14066193451115794 2306 9 -0.1448105409551843 2306 10-11 -0.14019147556013678 2306 12-13 -0.15321949954832093 2306 14-15 -0.24010707139205323 2306 16-17 -0.20344615331832472 2306 18-19 -0.1649185378165825 2306 20-21 -0.1275394806592871 2306 22-23 -0.2020580007546542 2306 24-25 -0.23348731082109708 2306 26-27 -0.14887515432642573 2306 28-29 -0.14250333753810907 2306 30-31 -0.1422899803811788 2306 32-33 -0.1624898511648425 2306 34-35 -0.16218890033437106 2306 36-37 -0.40031714557208886 2306 38-39 -0.3654169078652316 2306 40-41 -0.2823936457883107 2306 42-43 -0.25216072984041205 2306 44-45 -0.15297485539700517 2306 46-47 -0.28598332009206473 2306 48-49 -0.2888044579205058 2306 50-51 -0.4382455547421813 2306 52-53 -0.16095985376716726 2306 54-55 -0.33272440941603776 2306 56-57 -0.2562147735149978 2306 58-59 -0.15843632767037263 2306 60-61 -0.09744194570993159 2306 62-63 -0.13977241567491916 2306 64-65 -0.13655880320641955 2306 66-67 -0.15111491128070043 2306 68-69 -0.08199287454780091 2306 70-71 -0.3800128008307553 2306 72-73 -0.32464634829512207 2306 74-75 -0.1778202503947668 2306 76-77 -0.09242746747439057 2306 78-79 -0.12262907604282347 2306 80-81 -0.06591477719510763 2306 82-83 0.0675164863198745 2306 84-85 -0.06515961494507394 2306 86-87 -0.1844088113925757 2306 88-89 -0.2915811286536467 2306 90-91 -0.24669645244874872 2306 92-93 -0.06273464054063993 2306 94-95 0.03449096523476669 2306 96-97 0.08442967717701677 2306 98-99 0.02264622270102734 2306 100-101 0.1160728490148557 2307 1 -0.4072772875684194 2307 2 -0.3697488960289874 2307 3 -0.5378056086912508 2307 4 -0.32428744793556064 2307 5 -0.4262707895187887 2307 6 -0.3320899526880652 2307 7 -0.26193839966457944 2307 8 -0.2706899809111789 2307 9 -0.3156610608782273 2307 10-11 -0.46463463086262635 2307 12-13 -0.2800677787637298 2307 14-15 -0.5079061684516546 2307 16-17 -0.5905673838633732 2307 18-19 -0.6128741850526822 2307 20-21 -0.7188539762562343 2307 22-23 -0.6622791557448622 2307 24-25 -0.48028264804615617 2307 26-27 -0.3517143175802673 2307 28-29 -0.3646731605847364 2307 30-31 -0.2954861343787485 2307 32-33 -0.390328278328532 2307 34-35 -0.28793073711146633 2307 36-37 -0.4826839403421488 2307 38-39 -0.4177393102132072 2307 40-41 -0.39156234019484515 2307 42-43 -0.5100811417709536 2307 44-45 -0.5640237502501009 2307 46-47 -0.351871789398146 2307 48-49 -0.44347591929445684 2307 50-51 -0.31451782310421805 2307 52-53 -0.33124913082456686 2307 54-55 -0.41455386545617046 2307 56-57 -0.27126798597254265 2307 58-59 -0.2964046316116722 2307 60-61 -0.31769256817385383 2307 62-63 -0.2285314388810349 2307 64-65 -0.2668629978630861 2307 66-67 -0.3433581641995005 2307 68-69 -0.38041533306516584 2307 70-71 -0.3616614500714519 2307 72-73 -0.38784634600813206 2307 74-75 -0.4064359065006222 2307 76-77 -0.730316850145801 2307 78-79 -0.7096960756054038 2307 80-81 -0.6058137058651951 2307 82-83 -0.47812492989370625 2307 84-85 -0.44888983138875105 2307 86-87 -0.4948963447156167 2307 88-89 -0.5004281831849511 2307 90-91 -0.4830027332661402 2307 92-93 -0.4462022607423428 2307 94-95 -0.3686657641991431 2307 96-97 -0.40559267157847145 2307 98-99 -0.4790825788179234 2307 100-101 -0.4050194648076513 2308 1 -0.5264055975010677 2308 2 -0.4759996415467498 2308 3 -0.4342488602898946 2308 4 -0.6808481567619182 2308 5 -0.4527824974751766 2308 6 -0.7529909646941206 2308 7 -1.3404511457336739 2308 8 -0.53312732645783 2308 9 -0.6339586968330977 2308 10-11 -0.4026197395144422 2308 12-13 -0.3655437989103518 2308 14-15 -0.9626098011708422 2308 16-17 -1.1939321898767474 2308 18-19 -0.6496529739404835 2308 20-21 -0.539285852104463 2308 22-23 -0.5417563413956614 2308 24-25 -0.553938390386044 2308 26-27 -0.5208002632013162 2308 28-29 -0.8408671329450641 2308 30-31 -1.3786197178384256 2308 32-33 -0.6795148813297089 2308 34-35 -1.017961087489887 2308 36-37 -1.2540752080872366 2308 38-39 -0.8246142543686332 2308 40-41 -0.8336962690402672 2308 42-43 -0.6357044324250154 2308 44-45 -0.83572710818855 2308 46-47 -1.0398135514474163 2308 48-49 -1.7751871663263046 2308 50-51 -2.6267992659765866 2308 52-53 -1.8383707088768517 2308 54-55 -1.3530617119336554 2308 56-57 -0.597406830757194 2308 58-59 -0.7300646565314821 2308 60-61 -0.2141476079156419 2308 62-63 -0.20905603869231015 2308 64-65 -0.2563734133670721 2308 66-67 -0.28642499372178776 2308 68-69 -0.6769866220840512 2308 70-71 -0.8590451119514526 2308 72-73 -0.946689625223037 2308 74-75 -1.0225181687196638 2308 76-77 -0.7129519734231202 2308 78-79 -0.3985501975654522 2308 80-81 -0.5782602441428502 2308 82-83 -0.5489037421687684 2308 84-85 -0.9211827529206893 2308 86-87 -0.721120149519141 2308 88-89 -0.8350022255907739 2308 90-91 -1.1943840528222118 2308 92-93 -0.8472978990654987 2308 94-95 -1.7308792093334802 2308 96-97 -1.1130910856381284 2308 98-99 -1.55224232289509 2308 100-101 -1.7497000679861756 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 185.0 11 1575.0 12 4430.0 13 8432.0 14 13486.0 15 19974.0 16 28048.0 17 43890.0 18 88610.0 19 224987.0 20 232274.0 21 156186.0 22 137975.0 23 133543.0 24 139631.0 25 152073.0 26 170882.0 27 192022.0 28 210118.0 29 232218.0 30 259347.0 31 300937.0 32 364766.0 33 472629.0 34 684696.0 35 1169846.0 36 1840008.0 37 2533091.0 38 2102135.0 39 314735.0 40 184.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.085225141740533 37.775899057918814 16.950848023480255 17.188027776860398 2 21.066800425998185 24.753713668924075 38.373826812838416 15.805659092239333 3 20.708177837565465 32.42392083784439 21.28220286970008 25.58569845489007 4 28.114727510595184 21.50931143482061 31.593628207697982 18.78233284688622 5 20.454959389895265 29.7085339260075 19.933799921457812 29.902706762639426 6 20.55422435664563 21.374601117182525 29.602083428023214 28.469091098148635 7 27.870272304657366 21.577725212860123 20.213283570327796 30.338718912154718 8 37.80421919060175 21.374414847156217 20.25949661221385 20.561869350028182 9 20.122348588022675 31.267728806443206 20.634867434714664 27.975055170819452 10-11 34.49608825892371 21.240463026150103 20.45938597142333 23.804062743502858 12-13 29.1596688115629 21.120403436139302 20.744372866156592 28.975554886141204 14-15 28.870397704030665 21.129365230661275 20.86857704246477 29.131660022843285 16-17 29.02528309685115 24.961906057394657 20.600264074403846 25.412546771350346 18-19 25.333710294422033 21.258488164228382 24.500429823927508 28.907371717422077 20-21 25.096068672424465 29.935123399850045 21.269389081066198 23.69941884665929 22-23 19.553318276688895 24.72718279108595 30.372929888507365 25.346569043717786 24-25 19.64442492697571 21.19794250201669 33.96861386115951 25.18901870984809 26-27 19.51661702053044 21.1482894215922 39.48169237586906 19.8534011820083 28-29 19.699934995057596 20.979952776590636 39.746377723839515 19.573734504512252 30-31 19.67183617993302 20.839089677240217 39.759424847794016 19.729649295032743 32-33 19.626304184775247 20.847532125373743 39.85002347713472 19.67614021271629 34-35 19.64907502034399 20.98784012086919 39.727097678984705 19.635987179802118 36-37 19.7773734147077 20.873192066399834 39.55204096305626 19.79739355583621 38-39 19.77766628074159 20.976013717541676 39.05666558689395 20.189654414822794 40-41 20.780585517413165 21.94756720561632 36.17575381379615 21.096093463174363 42-43 20.88658713720761 22.18275714695859 36.14155794639512 20.789097769438687 44-45 20.94610677158326 22.898315905845994 35.45021075958103 20.70536656298971 46-47 20.78107608165358 23.55706630854611 35.11190286535687 20.54995474444344 48-49 20.72324136478474 23.850821633322937 34.73801444380196 20.687922558090364 50-51 20.64799257881484 24.1293115763499 34.43677206240155 20.785923782433706 52-53 20.680917269488592 24.28552118463373 34.25677399846011 20.77678754741757 54-55 20.879918498059016 24.04331374466472 33.97074063969214 21.106027117584127 56-57 20.83685525184674 23.925252163915783 34.02072735560352 21.21716522863396 58-59 20.94799442061935 24.03904422001686 33.72595081509297 21.287010544270824 60-61 21.165854958913503 23.936362014055433 33.38373041574691 21.514052611284157 62-63 21.338064448411156 24.091230578220273 33.03918297158257 21.531522001786 64-65 21.36622295805117 24.296133656772174 32.64899933298812 21.68864405218854 66-67 21.517886141045626 24.252125843840716 32.272627430258396 21.95736058485526 68-69 21.657395591986383 24.537241303252884 31.889153247295994 21.91620985746474 70-71 21.833810007488054 24.77550860741944 31.62324973029068 21.76743165480183 72-73 22.005414887843283 24.76521902059673 31.417737121656604 21.81162896990338 74-75 21.93465965119523 24.87196492128032 31.252363838507907 21.941011589016544 76-77 22.02493906660323 24.98044799682327 31.076723336129437 21.917889600444063 78-79 22.15710256756619 24.790307800482342 31.0144470916813 22.038142540270165 80-81 21.983994866422965 24.89384108157127 31.027475203959256 22.094688848046513 82-83 22.142322895256118 25.019937137167297 30.824700378886334 22.01303958869025 84-85 22.231087731345237 24.83054574586358 30.7110064743967 22.22736004839448 86-87 22.169486662117396 24.95591643159041 30.758270893277523 22.11632601301467 88-89 22.251564914043055 25.11159446982909 30.559447522456846 22.07739309367101 90-91 22.46788092062432 24.885599639529936 30.399301360362486 22.247218079483254 92-93 22.435832641177427 24.912940813323008 30.377967603547596 22.273258941951973 94-95 22.493505529532168 25.08348281743182 30.20409744814121 22.218914204894805 96-97 22.3973145658365 25.086893523428145 30.098897893528985 22.416894017206374 98-99 22.494495621450643 25.05894928140306 30.1256915701332 22.320863527013096 100-101 22.49455720377302 25.324868464480556 29.678320677925228 22.502253653821196 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 91181.0 1 51793.5 2 8639.5 3 3864.0 4 2392.5 5 1790.0 6 1523.5 7 1366.5 8 1268.5 9 1203.5 10 1320.5 11 1525.0 12 3057.0 13 5874.0 14 8379.0 15 13955.0 16 402119.5 17 441964.0 18 86073.5 19 64086.5 20 46117.5 21 34756.5 22 30579.0 23 30890.0 24 33615.0 25 37761.0 26 43749.0 27 51416.5 28 60492.5 29 71211.0 30 83945.0 31 96703.5 32 109922.5 33 126302.0 34 143291.5 35 160308.0 36 182167.5 37 204659.5 38 227451.5 39 249804.5 40 266559.0 41 279472.0 42 295762.0 43 314969.0 44 336337.5 45 361658.5 46 387391.5 47 414996.0 48 441046.0 49 461460.0 50 463484.5 51 461093.5 52 471383.5 53 481045.0 54 474808.0 55 456477.5 56 431721.0 57 396943.0 58 355185.0 59 311088.5 60 268869.0 61 227123.5 62 185100.5 63 141426.5 64 102500.5 65 72915.5 66 51076.0 67 37136.5 68 27573.5 69 20363.0 70 15476.0 71 11790.5 72 9558.5 73 7409.5 74 5427.5 75 4418.5 76 3512.5 77 2235.5 78 1289.5 79 769.0 80 425.5 81 258.5 82 184.0 83 128.5 84 67.5 85 34.5 86 17.5 87 4.5 88 1.5 89 1.5 90 0.5 91 1.0 92 1.5 93 1.0 94 1.5 95 1.0 96 0.5 97 0.5 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 8.174665795138297E-6 9 0.0 10-11 0.0 12-13 8.174665795138297E-6 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 5.7222660565968083E-5 28-29 0.0 30-31 0.003249429653567473 32-33 0.0065519946348033455 34-35 0.0015327498365884308 36-37 0.0019210464618574998 38-39 0.0032984776483383034 40-41 0.0034251849681629465 42-43 0.0024523997385414893 44-45 0.006952553258765122 46-47 0.0013406451904026807 48-49 4.618686174253138E-4 50-51 0.0030123643455084626 52-53 0.0019660071237307605 54-55 6.417112649183563E-4 56-57 0.0 58-59 1.3896931851735108E-4 60-61 5.313532766839893E-4 62-63 0.0 64-65 4.0873328975691486E-6 66-67 7.357199215624467E-5 68-69 0.006417112649183564 70-71 0.006257706666178367 72-73 0.0028202596993227125 74-75 0.0035028442932167605 76-77 0.032616916522601805 78-79 0.056899761267060124 80-81 0.0487537068022048 82-83 7.479819202551542E-4 84-85 9.482612322360425E-4 86-87 0.0015000511734078775 88-89 0.0037194729367879256 90-91 0.0 92-93 2.0436664487845745E-5 94-95 7.765932505381382E-5 96-97 0.005754964719777361 98-99 0.0012629858653488669 100-101 2.8611330282984042E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.2232916E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.720463113578376 #Duplication Level Percentage of deduplicated Percentage of total 1 75.63651963324516 33.068636666647414 2 13.225410455323635 11.564421399478217 3 4.172267800559876 5.472404414230465 4 1.878244457601725 3.2847087010743703 5 1.0719151040098212 2.3432312382874456 6 0.6560583922591129 1.7209906043470846 7 0.4668490134530762 1.4287598550599971 8 0.3406041180933563 1.191309582514678 9 0.259152437009506 1.0197238106761244 >10 2.0864229615633874 18.609594890895526 >50 0.17815873809578694 5.1691558115801355 >100 0.025285361100870356 1.7284260406334346 >500 0.0015574021091974309 0.45924625107149575 >1k 0.0011234655264790823 1.018190417946757 >5k 2.059678494949067E-4 0.685944339398662 >10k+ 2.246921994489891E-4 11.235255976158099 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 1053668 8.61338375903178 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT 104755 0.8563371153697122 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 75202 0.6147512171259902 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32581 0.2663387862714009 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22821 0.18655404811085108 No Hit GTCGACGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17549 0.14345721003888198 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 9.809598954165957E-5 0.0 0.0 0.0 8.174665795138297E-6 2 9.809598954165957E-5 0.0 0.0 0.0 2.452399738541489E-5 3 9.809598954165957E-5 0.0 0.0 0.0 3.269866318055319E-5 4 1.1444532113193617E-4 0.0 0.0 0.0 4.904799477082978E-5 5 1.1444532113193617E-4 0.0 0.0 8.174665795138297E-6 7.357199215624467E-5 6 1.1444532113193617E-4 0.0 0.0 8.174665795138297E-6 7.357199215624467E-5 7 1.1444532113193617E-4 0.0 0.0 8.174665795138297E-6 7.357199215624467E-5 8 1.2261998692707446E-4 0.0 0.0 8.174665795138297E-6 1.0627065533679786E-4 9 1.2261998692707446E-4 0.0 0.0 8.174665795138297E-6 1.3079465272221275E-4 10-11 1.4714398431248934E-4 4.0873328975691486E-6 0.0 8.174665795138297E-6 1.675806488003351E-4 12-13 1.7166798169790425E-4 8.174665795138297E-6 0.0 2.452399738541489E-5 1.8801731328818084E-4 14-15 2.0436664487845743E-4 8.174665795138297E-6 0.0 3.269866318055319E-5 2.329779751614415E-4 16-17 2.1662864357116488E-4 2.043666448784574E-5 0.0 3.269866318055319E-5 2.6567663834199467E-4 18-19 2.493273067517181E-4 2.452399738541489E-5 0.0 3.269866318055319E-5 3.147246331128245E-4 20-21 3.106373002152553E-4 2.452399738541489E-5 0.0 4.904799477082979E-5 3.31073964703101E-4 22-23 4.373446200398989E-4 2.452399738541489E-5 0.0 6.130999346353723E-5 3.433359633958085E-4 24-25 5.395279424791277E-4 2.452399738541489E-5 0.0 6.539732636110638E-5 3.8012195947393084E-4 26-27 6.784972609964787E-4 2.8611330282984042E-5 0.0 6.539732636110638E-5 4.1690795555205317E-4 28-29 8.174665795138297E-4 3.269866318055319E-5 0.0 7.357199215624467E-5 4.7821794901559036E-4 30-31 9.07387903260351E-4 3.269866318055319E-5 0.0 7.357199215624467E-5 5.068292792985744E-4 32-33 0.0010177458914947181 3.269866318055319E-5 0.0 7.357199215624467E-5 5.313532766839893E-4 34-35 0.0011444532113193615 3.269866318055319E-5 0.0 7.357199215624467E-5 5.599646069669734E-4 36-37 0.0012752478640415744 3.269866318055319E-5 0.0 7.765932505381383E-5 5.80401271454819E-4 38-39 0.0014060425167637873 4.087332897569149E-5 0.0 9.400865664409042E-5 6.335365991232181E-4 40-41 0.0015164005049981542 4.087332897569149E-5 0.0 1.675806488003351E-4 6.539732636110637E-4 42-43 0.0016553698235155052 4.087332897569149E-5 0.0 1.8392998039061168E-4 6.907592596891862E-4 44-45 0.0017943391420328562 4.087332897569149E-5 0.0 1.8801731328818084E-4 7.275452557673084E-4 46-47 0.001978269122423468 4.087332897569149E-5 0.0 1.8801731328818084E-4 7.888552492308457E-4 48-49 0.002178548434404356 4.904799477082978E-5 0.0 1.8801731328818084E-4 8.092919137186914E-4 50-51 0.002346129083204691 4.904799477082978E-5 0.0 1.8801731328818084E-4 8.460779097968138E-4 52-53 0.0027385130413713297 4.904799477082978E-5 0.0 1.9210464618575E-4 8.706019071822287E-4 54-55 0.003208556324591782 4.904799477082978E-5 0.0 2.0436664487845743E-4 9.033005703627819E-4 56-57 0.003915664915871244 4.904799477082978E-5 0.0 2.0845397777602659E-4 9.523485651336116E-4 58-59 0.004659559503228829 4.904799477082978E-5 0.0 2.1254131067359572E-4 0.0010013965599044413 60-61 0.005456589418254814 4.904799477082978E-5 0.0 2.1662864357116488E-4 0.0010586192204704095 62-63 0.006633741292754729 4.904799477082978E-5 0.0 2.2071597646873404E-4 0.001128103879729085 64-65 0.008182840460933436 4.904799477082978E-5 0.0 2.2889064226387233E-4 0.0011771518744999147 66-67 0.010148847584664196 4.904799477082978E-5 0.0 2.2889064226387233E-4 0.0012139378705780372 68-69 0.012617596654795962 5.3135327668398937E-5 0.0 2.329779751614415E-4 0.0012507238666561596 70-71 0.015654484997689837 5.7222660565968083E-5 0.0 2.3706530805901063E-4 0.0012997718614269893 72-73 0.01968868256759059 5.7222660565968083E-5 0.0 2.534146396492872E-4 0.0013529071890953882 74-75 0.024613918709161412 5.7222660565968083E-5 0.0 2.6976397123956383E-4 0.0014142171825589254 76-77 0.030949284700393594 6.539732636110638E-5 0.0 2.6976397123956383E-4 0.0014183045154564946 78-79 0.0390830771665562 6.539732636110638E-5 0.0 2.779386370347021E-4 0.0014755271760224626 80-81 0.04937906873553288 6.539732636110638E-5 0.0 2.8202596993227126E-4 0.0015531865010762766 82-83 0.06198849072453371 6.539732636110638E-5 0.0 2.861133028298404E-4 0.001622671160334952 84-85 0.07765523772091626 6.948465925867552E-5 0.0 2.861133028298404E-4 0.0016431078248227977 86-87 0.0963711350588854 7.357199215624467E-5 0.0 2.942879686249787E-4 0.0016798938209009202 88-89 0.11872884600858863 7.357199215624467E-5 0.0 2.9837530152254784E-4 0.0017575531459547338 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 90165 0.0 94.23091 1 ATATCTC 133705 0.0 92.73571 1 TATCTCG 133825 0.0 92.535446 2 TCGACGG 92840 0.0 91.500496 2 ATCTCGA 138965 0.0 89.047844 3 ACGGCGC 95305 0.0 88.984406 5 GACGGCG 95635 0.0 88.72204 4 CGACGGC 95765 0.0 88.58673 3 CGGCGCG 96165 0.0 88.262695 6 TCTCGAG 138995 0.0 87.75073 4 TCGAGGG 140975 0.0 86.49468 6 CTCGAGG 141100 0.0 86.387764 5 CGAGGGC 142525 0.0 85.46739 7 AGGGCGC 143670 0.0 84.95814 9 GAGGGCG 144980 0.0 84.18065 8 CTCGACG 3475 0.0 49.063744 5 TCTCGAC 3790 0.0 45.361816 4 AATTCGA 4625 0.0 44.668217 1 GGGCGCG 144345 0.0 42.39723 10-11 GGATCCT 142790 0.0 41.24915 18-19 >>END_MODULE