FastQCFastQC Report
Tue 31 May 2016
SRR1051266_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051266_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12232916
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5844974.7780676332609495No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1141760.9333506418257103No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT489400.40006814401406826No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135210.11052965621606492No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127670.10436595820653065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG121550.090.746841
TCGACGG124550.088.70832
ACGGCGC152150.072.990075
CGACGGC153650.072.463293
GACGGCG158800.070.0235064
CGGCGCG163350.067.9855356
ATCTCGA97900.058.980021
TCTCGAG120150.048.073762
AATTCGA50700.042.180911
TCGAGGG141350.040.8630454
CTCGAGG143850.040.417043
CGCGCCG324850.039.2602049
GGCGCGC347750.036.647537
GCGCGCC350300.036.4349948
CGAGGGC158650.036.34715
TATCTCG34100.034.9637222
GGGCGCG168950.034.074988
AGGGCGC171100.033.7300837
CGAGGCG33500.031.4774345
GCGGTAC33250.031.4283969
Produced by FastQC (version 0.11.5)