FastQCFastQC Report
Tue 31 May 2016
SRR1051265_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051265_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10780618
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT7257246.73174766047735No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT743330.6895059262836323No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA468660.43472461411766933No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT268750.24928997576947812No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128430.11913046172306634No Hit
GTCGACGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127260.11804518071227457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG638050.094.0393141
TATCTCG930600.092.320642
ATATCTC933000.092.190061
TCGACGG657600.091.337482
ATCTCGA971950.088.2659763
ACGGCGC681550.088.016345
GACGGCG684100.087.750744
CGACGGC685200.087.561353
CGGCGCG689350.087.0548866
TCTCGAG976500.086.6534044
CTCGAGG1003500.084.298265
TCGAGGG1003850.084.278326
CGAGGGC1017800.083.15127
AGGGCGC1024300.082.7440959
GAGGGCG1044850.081.2257848
CTCGACG26100.043.3071635
GGGCGCG1030850.041.20361310-11
GATCCTT1019350.039.8700620-21
GGATCCT1033150.039.8569518-19
TCCATAT746100.039.56419818-19
Produced by FastQC (version 0.11.5)