##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051265_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10780618 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04400044598557 33.0 31.0 34.0 30.0 34.0 2 32.28829701599667 34.0 31.0 34.0 30.0 34.0 3 32.36933346492752 34.0 31.0 34.0 30.0 34.0 4 35.78694588751777 37.0 35.0 37.0 35.0 37.0 5 35.78298034491158 37.0 35.0 37.0 35.0 37.0 6 35.77851983995723 37.0 35.0 37.0 35.0 37.0 7 35.72731349909625 37.0 35.0 37.0 33.0 37.0 8 35.70747623188207 37.0 35.0 37.0 33.0 37.0 9 37.394603352052734 39.0 37.0 39.0 34.0 39.0 10-11 37.364938494249586 39.0 37.0 39.0 34.0 39.0 12-13 37.31969767410365 39.0 37.0 39.0 34.0 39.0 14-15 38.667401998660935 40.0 38.0 41.0 34.0 41.0 16-17 38.543341995792815 40.0 38.0 41.0 33.5 41.0 18-19 38.544343793648935 40.0 38.0 41.0 34.0 41.0 20-21 38.51246950777775 40.0 38.0 41.0 34.0 41.0 22-23 38.48904687096788 40.0 38.0 41.0 34.0 41.0 24-25 38.4808603273022 40.0 38.0 41.0 34.0 41.0 26-27 38.39423199115301 40.0 38.0 41.0 34.0 41.0 28-29 38.17133832216298 40.0 38.0 41.0 33.5 41.0 30-31 37.63760973628784 39.5 36.5 41.0 33.0 41.0 32-33 37.152252867136184 39.0 35.5 41.0 32.5 41.0 34-35 36.886437864693846 38.5 35.0 40.0 32.0 41.0 36-37 37.01783376426101 39.0 35.0 41.0 32.0 41.0 38-39 36.87565652544224 39.0 35.0 41.0 31.5 41.0 40-41 36.224075883219314 39.0 35.0 41.0 29.0 41.0 42-43 36.22019196858659 39.0 35.0 41.0 29.0 41.0 44-45 36.13491448263912 39.0 35.0 41.0 28.5 41.0 46-47 36.133564745546124 39.0 35.0 41.0 28.5 41.0 48-49 35.96266512736097 39.0 35.0 41.0 27.0 41.0 50-51 35.727216055702925 38.5 34.5 40.5 27.0 41.0 52-53 35.54332182069711 38.0 34.5 40.0 27.5 41.0 54-55 35.27454465040873 38.0 34.5 40.0 26.5 41.0 56-57 34.88787154873682 37.5 34.0 40.0 26.0 41.0 58-59 34.57717303405055 37.0 34.0 40.0 26.0 41.0 60-61 34.39915907418295 37.0 34.0 40.0 26.0 41.0 62-63 34.088557678233286 36.0 34.0 39.5 25.0 41.0 64-65 33.7375745527761 36.0 34.0 39.0 25.0 41.0 66-67 33.28506426997042 35.0 33.5 39.0 23.0 41.0 68-69 32.8484508494782 35.0 33.0 38.5 22.0 40.5 70-71 32.38964723543678 35.0 33.0 37.0 20.5 39.5 72-73 32.01899705564189 35.0 33.0 37.0 20.0 39.0 74-75 31.65901263730892 35.0 32.0 36.0 19.0 39.0 76-77 31.23573087368461 35.0 32.0 36.0 17.0 38.0 78-79 30.951989069643318 35.0 32.0 35.0 16.0 37.0 80-81 30.702186229026942 35.0 32.0 35.0 11.0 37.0 82-83 30.434295510702633 35.0 31.5 35.0 7.5 36.0 84-85 30.14136350995833 35.0 31.0 35.0 6.5 36.0 86-87 29.882311385117255 35.0 31.0 35.0 2.0 36.0 88-89 29.619877404059768 35.0 31.0 35.0 2.0 35.0 90-91 29.42262878621615 34.0 31.0 35.0 2.0 35.0 92-93 29.23474976109904 34.0 30.5 35.0 2.0 35.0 94-95 28.947471285968952 34.0 30.0 35.0 2.0 35.0 96-97 28.635551783766015 34.0 30.0 35.0 2.0 35.0 98-99 28.29804390620278 34.0 30.0 35.0 2.0 35.0 100-101 27.11118861645965 33.5 27.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3534785801830438 1101 2 0.34407469037651595 1101 3 0.3757256792808832 1101 4 0.3354693087493388 1101 5 0.3471371823931193 1101 6 0.34256980196892783 1101 7 0.38538856470532323 1101 8 0.2920917094373294 1101 9 0.4040376078326773 1101 10-11 0.3388138204961706 1101 12-13 0.39157977814417677 1101 14-15 0.5097116843040226 1101 16-17 0.4350809047674389 1101 18-19 0.50473125691191 1101 20-21 0.49727977592323214 1101 22-23 0.48150280476182417 1101 24-25 0.5094076405740822 1101 26-27 0.5453634082473755 1101 28-29 0.6223053114868193 1101 30-31 0.6730444766689132 1101 32-33 0.6720712709812773 1101 34-35 0.7034336835490151 1101 36-37 0.7165279336722392 1101 38-39 0.7235178401292686 1101 40-41 0.7426172870589198 1101 42-43 0.6976579240687997 1101 44-45 0.7240064779417139 1101 46-47 0.6849373607821079 1101 48-49 0.6566194687816989 1101 50-51 0.61255354009689 1101 52-53 0.5167952529546369 1101 54-55 0.562280788199871 1101 56-57 0.511721751621323 1101 58-59 0.48530928812761687 1101 60-61 0.5071235478304388 1101 62-63 0.5260832816546781 1101 64-65 0.479702122979063 1101 66-67 0.4586402991781995 1101 68-69 0.4487585612920384 1101 70-71 0.5103874274326898 1101 72-73 0.5606241852799272 1101 74-75 0.5091916801020311 1101 76-77 0.3589492501376732 1101 78-79 0.27597671050894235 1101 80-81 0.28216053676073827 1101 82-83 0.3554317037273016 1101 84-85 0.33715495264708295 1101 86-87 0.3307352567826811 1101 88-89 0.3733959027652425 1101 90-91 0.428586167284319 1101 92-93 0.44174652884533216 1101 94-95 0.47009544384226487 1101 96-97 0.43013959249429234 1101 98-99 0.4855281826425113 1101 100-101 0.6342466030765017 1102 1 0.3329890454983939 1102 2 0.31600561678110495 1102 3 0.32993571348485773 1102 4 0.2515266689494169 1102 5 0.2528410216906991 1102 6 0.26029304067964176 1102 7 0.2816377640784751 1102 8 0.27089194839825836 1102 9 0.3173313058966869 1102 10-11 0.32504612739096217 1102 12-13 0.32866808234766154 1102 14-15 0.4709861625347571 1102 16-17 0.42747671552859146 1102 18-19 0.46955113233138235 1102 20-21 0.3762914043174348 1102 22-23 0.41625727719811323 1102 24-25 0.44361142539910503 1102 26-27 0.4957171087297283 1102 28-29 0.5108079147961249 1102 30-31 0.5421872103292387 1102 32-33 0.5483794324068114 1102 34-35 0.5997426351258994 1102 36-37 0.6736052236446213 1102 38-39 0.629713117657964 1102 40-41 0.5572014818767244 1102 42-43 0.5611831484538286 1102 44-45 0.5447002906973921 1102 46-47 0.4893327812563655 1102 48-49 0.45931034121654335 1102 50-51 0.46400179176728074 1102 52-53 0.4017688966179307 1102 54-55 0.393400109435909 1102 56-57 0.3924269018518416 1102 58-59 0.35184649767216314 1102 60-61 0.28770246539490074 1102 62-63 0.3684059914428559 1102 64-65 0.32740438676271566 1102 66-67 0.2603697369855027 1102 68-69 0.2818187217406489 1102 70-71 0.3613183574288783 1102 72-73 0.38696723308527936 1102 74-75 0.378271967860794 1102 76-77 0.22074848464861319 1102 78-79 0.15987346166992467 1102 80-81 0.23938034130602404 1102 82-83 0.31190952022781815 1102 84-85 0.22464271427996607 1102 86-87 0.21640149205862969 1102 88-89 0.3577568319353581 1102 90-91 0.4126044341471662 1102 92-93 0.3112800761580381 1102 94-95 0.3823319912160308 1102 96-97 0.3694509875544085 1102 98-99 0.47310671590608777 1102 100-101 0.5231536681071525 1103 1 0.2730028460901224 1103 2 0.2720463984744299 1103 3 0.3113452896768365 1103 4 0.2493135939770923 1103 5 0.2701962576855692 1103 6 0.2753018889242682 1103 7 0.30906948158576597 1103 8 0.2965555623615259 1103 9 0.35324787744198005 1103 10-11 0.3273756518431057 1103 12-13 0.32761491973178636 1103 14-15 0.42142081497490125 1103 16-17 0.4785279239484055 1103 18-19 0.5013348839112552 1103 20-21 0.46266151813406964 1103 22-23 0.4756806791024957 1103 24-25 0.4689658281151381 1103 26-27 0.47030304878121854 1103 28-29 0.5038451767225993 1103 30-31 0.5152869311236117 1103 32-33 0.4689510032974482 1103 34-35 0.5175662907097092 1103 36-37 0.44677174221398985 1103 38-39 0.4331682404059123 1103 40-41 0.4654259006557453 1103 42-43 0.45112319814673896 1103 44-45 0.4134131186387222 1103 46-47 0.3901853521684231 1103 48-49 0.4397177798051999 1103 50-51 0.3672619815406577 1103 52-53 0.4004780520297331 1103 54-55 0.40781698423779034 1103 56-57 0.4563817194361306 1103 58-59 0.4222112799860156 1103 60-61 0.3585692338755848 1103 62-63 0.47915639768656604 1103 64-65 0.4799234408318682 1103 66-67 0.39025571978380214 1103 68-69 0.39533552102047054 1103 70-71 0.45349134518727396 1103 72-73 0.460442905909062 1103 74-75 0.43602729300119947 1103 76-77 0.45516386974590617 1103 78-79 0.36850423376281327 1103 80-81 0.3901729426211169 1103 82-83 0.49140268331453996 1103 84-85 0.431587650865918 1103 86-87 0.4899103522388728 1103 88-89 0.5569750581597113 1103 90-91 0.5330295028065244 1103 92-93 0.4464183033609146 1103 94-95 0.517984625732069 1103 96-97 0.48519299250870773 1103 98-99 0.5627345172921672 1103 100-101 0.717494361266521 1104 1 0.2942579732409598 1104 2 0.2641024711508493 1104 3 0.2241147838878632 1104 4 0.19107305885051318 1104 5 0.21982416233026214 1104 6 0.2245201909470893 1104 7 0.26059348219603606 1104 8 0.2192062985181309 1104 9 0.22891635058942938 1104 10-11 0.24112431354485153 1104 12-13 0.2958732704850462 1104 14-15 0.36619396529319914 1104 16-17 0.4374654960603621 1104 18-19 0.4379126979858867 1104 20-21 0.45092869823814397 1104 22-23 0.4688356793293309 1104 24-25 0.43299635381846713 1104 26-27 0.41810240766625384 1104 28-29 0.4028883340044942 1104 30-31 0.47596312647036854 1104 32-33 0.4216849587704701 1104 34-35 0.4942553508541536 1104 36-37 0.47243334191941955 1104 38-39 0.4311752354954095 1104 40-41 0.388852025015737 1104 42-43 0.3656926969973071 1104 44-45 0.3724612048294276 1104 46-47 0.3306732885894732 1104 48-49 0.31483046651727165 1104 50-51 0.23614118186299038 1104 52-53 0.27199884899592774 1104 54-55 0.22137017970334938 1104 56-57 0.18716141629109018 1104 58-59 0.26099061422699066 1104 60-61 0.13263992330700347 1104 62-63 0.21154949544480672 1104 64-65 0.14423989052555442 1104 66-67 0.2333348750833295 1104 68-69 0.2737457235746419 1104 70-71 0.3191966267593216 1104 72-73 0.2915665668084628 1104 74-75 0.2552182406288459 1104 76-77 0.3592338956840422 1104 78-79 0.2575599719553452 1104 80-81 0.24380864751882925 1104 82-83 0.2593497794445341 1104 84-85 0.2778199778365291 1104 86-87 0.32874338715501494 1104 88-89 0.3353751573439929 1104 90-91 0.440458560409823 1104 92-93 0.3400285875543325 1104 94-95 0.46727959285613707 1104 96-97 0.4908577705298143 1104 98-99 0.5235153967395227 1104 100-101 0.60155570004844 1105 1 0.04385450096263099 1105 2 0.07277472286590125 1105 3 0.11197523103039231 1105 4 -0.06684140314474263 1105 5 0.06782820024916703 1105 6 0.05145679145362436 1105 7 0.08307778623038331 1105 8 0.12115447657355816 1105 9 0.1329209707407486 1105 10-11 0.04688722507034271 1105 12-13 0.13435895926400576 1105 14-15 0.21025104238747616 1105 16-17 0.0632792233716728 1105 18-19 0.1426666455896921 1105 20-21 0.09558126054133709 1105 22-23 0.09194601799194402 1105 24-25 0.1074450998024048 1105 26-27 0.1328907134252546 1105 28-29 0.20898353478886378 1105 30-31 0.21102150127605057 1105 32-33 0.12840307194901612 1105 34-35 0.20947582549297294 1105 36-37 0.20965871586947316 1105 38-39 0.2440718033502023 1105 40-41 0.18243229315886822 1105 42-43 0.13568980298816058 1105 44-45 0.08710734348258597 1105 46-47 0.12050044298107565 1105 48-49 0.1353663024193068 1105 50-51 0.12200107390514603 1105 52-53 0.006731859646158966 1105 54-55 0.07356551493378305 1105 56-57 0.21482780942952928 1105 58-59 0.2507378488854144 1105 60-61 0.1116408204159356 1105 62-63 0.21553940855614684 1105 64-65 0.11487275673842134 1105 66-67 0.10965484491306654 1105 68-69 0.1580938938546268 1105 70-71 0.16136054114209486 1105 72-73 0.23111103498641228 1105 74-75 0.26002172297075177 1105 76-77 0.36384104530454664 1105 78-79 0.26547100489271 1105 80-81 0.18441723908735597 1105 82-83 0.2447022042104834 1105 84-85 0.19514035291928877 1105 86-87 0.2896093576678851 1105 88-89 0.3182373888044303 1105 90-91 0.41978095262916426 1105 92-93 0.3005185248492275 1105 94-95 0.3933545866697763 1105 96-97 0.2865541160286007 1105 98-99 0.3625643654947943 1105 100-101 0.41353422836686704 1106 1 -0.08581058476524817 1106 2 -0.13223226308360836 1106 3 -0.12230540481281338 1106 4 -0.27220715998345923 1106 5 -0.06390711343806288 1106 6 -0.04380965470741671 1106 7 -0.031531166194533 1106 8 -0.023958926117160217 1106 9 -0.03800831187867004 1106 10-11 -0.06607564097098617 1106 12-13 -0.056071545337310624 1106 14-15 -0.18896392119447114 1106 16-17 -0.13207090374167052 1106 18-19 -0.17672703464199913 1106 20-21 -0.2615104464518083 1106 22-23 -0.07240780584493223 1106 24-25 8.10298399471776E-5 1106 26-27 -0.3138898789661795 1106 28-29 -0.17210563036568516 1106 30-31 -0.12839964196040654 1106 32-33 -0.18519064776907612 1106 34-35 -0.09696947891579555 1106 36-37 -0.2984947436117622 1106 38-39 -0.1532061020560036 1106 40-41 -0.13777767437770194 1106 42-43 -0.16459476474582146 1106 44-45 -0.1885024199420826 1106 46-47 -0.04286429204049824 1106 48-49 -0.026582846290558848 1106 50-51 -0.16791092147608566 1106 52-53 -0.15629116742051252 1106 54-55 -0.07066419920634104 1106 56-57 0.019963672076791283 1106 58-59 -0.004745801256341053 1106 60-61 -0.04104249749430977 1106 62-63 0.08471328467992834 1106 64-65 0.05927565540517321 1106 66-67 7.966125147618186E-5 1106 68-69 0.12290160826523788 1106 70-71 0.21674905710134595 1106 72-73 0.11213366624493659 1106 74-75 0.1698914400370377 1106 76-77 0.26484451762643246 1106 78-79 0.07623085974750055 1106 80-81 -0.005441886906112359 1106 82-83 0.10592849375739988 1106 84-85 0.04748295548005288 1106 86-87 0.10861453015021993 1106 88-89 0.1922452777517627 1106 90-91 0.21877737882146775 1106 92-93 0.07225849349847735 1106 94-95 0.10547938961914127 1106 96-97 -0.05728849936286551 1106 98-99 0.05582034805053837 1106 100-101 0.12516929231235352 1107 1 -0.2421521076312949 1107 2 -0.30337969317136526 1107 3 -0.3081199334964566 1107 4 -0.2275390470108718 1107 5 -0.32484631544341624 1107 6 -0.2121921005542049 1107 7 -0.22961921613882197 1107 8 -0.20444369208426139 1107 9 -0.19732130299689743 1107 10-11 -0.25569770185987295 1107 12-13 -0.1838953445987741 1107 14-15 -0.28020068705107093 1107 16-17 -0.28650742366890114 1107 18-19 -0.3159319930083839 1107 20-21 -0.2957353162950511 1107 22-23 -0.2612366069528207 1107 24-25 -0.3788938029238622 1107 26-27 -0.27926948740967816 1107 28-29 -0.33343852502823523 1107 30-31 -0.18302197199916037 1107 32-33 -0.28223307530547714 1107 34-35 -0.4382664781509291 1107 36-37 -0.35149502478326866 1107 38-39 -0.3138308143205606 1107 40-41 -0.2820489617893571 1107 42-43 -0.319463192381356 1107 44-45 -0.29818561502428764 1107 46-47 -0.265759438061842 1107 48-49 -0.2720563235580826 1107 50-51 -0.26684743420928925 1107 52-53 -0.16585165311519035 1107 54-55 -0.1302034910792358 1107 56-57 -0.1245591766224976 1107 58-59 -0.123729530316254 1107 60-61 -0.183089926217896 1107 62-63 -0.17368266648524866 1107 64-65 -0.29027072873989823 1107 66-67 -0.1941135036679995 1107 68-69 -0.11687158250126117 1107 70-71 -0.011789280388001089 1107 72-73 -4.959214211197605E-4 1107 74-75 -0.07570169705105911 1107 76-77 -0.36521387615455936 1107 78-79 -0.4047238638602231 1107 80-81 -0.1987568592571236 1107 82-83 -0.14743156728220086 1107 84-85 -0.26673669946998046 1107 86-87 -0.2749257091127646 1107 88-89 -0.2014509437980827 1107 90-91 -0.09762070693485114 1107 92-93 -0.24003377185534447 1107 94-95 -0.15524186023699826 1107 96-97 -0.1945244860266513 1107 98-99 -0.26736627602824825 1107 100-101 -0.3809221648194665 1108 1 -0.49531538992099655 1108 2 -0.4272985768103368 1108 3 -0.4003955215244659 1108 4 -0.4852195563382722 1108 5 -0.7429951871176641 1108 6 -0.4719189114625806 1108 7 -0.4694522943160422 1108 8 -1.2513168723255816 1108 9 -0.7666480946873193 1108 10-11 -0.6207405109936062 1108 12-13 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2305 24-25 0.2006911230771138 2305 26-27 0.1361205960453944 2305 28-29 0.15454948908388388 2305 30-31 0.13262661236046824 2305 32-33 0.23724865549856844 2305 34-35 0.18059832437888446 2305 36-37 0.06457774444205455 2305 38-39 0.20684101367687902 2305 40-41 0.1928742417271394 2305 42-43 0.1572778167734441 2305 44-45 0.276291572879515 2305 46-47 0.19078946412482622 2305 48-49 0.1847370708051983 2305 50-51 0.18836929967162774 2305 52-53 0.19557146058371444 2305 54-55 0.15540705330462146 2305 56-57 0.24168252572354731 2305 58-59 0.21006411235664757 2305 60-61 0.28344724105163976 2305 62-63 0.28032037967268764 2305 64-65 0.19836172119458695 2305 66-67 0.2346650298548525 2305 68-69 0.14862628377520792 2305 70-71 0.2410721836367813 2305 72-73 0.2048868552449008 2305 74-75 0.2752104017049888 2305 76-77 0.3673095408433049 2305 78-79 0.3462265766957735 2305 80-81 0.3289806907488142 2305 82-83 0.27543031273802043 2305 84-85 0.249272561347329 2305 86-87 0.2184673433444999 2305 88-89 0.2528408646577027 2305 90-91 0.2932509333032307 2305 92-93 0.33599393131194333 2305 94-95 0.46677755605393045 2305 96-97 0.5875928246526314 2305 98-99 0.5352498957626572 2305 100-101 0.717904003528961 2306 1 -0.2830139475051112 2306 2 -0.5221488077153325 2306 3 -0.326119766797774 2306 4 -0.19067836092013124 2306 5 -0.2094303039351928 2306 6 -0.21389147097755767 2306 7 -0.10502043603079869 2306 8 -0.11298217260807775 2306 9 -0.14356282995061775 2306 10-11 -0.11757620473920838 2306 12-13 -0.13135364853302178 2306 14-15 -0.255252633890386 2306 16-17 -0.18776367014670114 2306 18-19 -0.1726963855540049 2306 20-21 -0.12149402466250336 2306 22-23 -0.17649600497628626 2306 24-25 -0.2109050610216059 2306 26-27 -0.1101931561468632 2306 28-29 -0.12197485281057396 2306 30-31 -0.16557165834426257 2306 32-33 -0.12428272421662712 2306 34-35 -0.11265449854553111 2306 36-37 -0.3362672316570965 2306 38-39 -0.2689779212773473 2306 40-41 -0.15430199002658185 2306 42-43 -0.1841078369858664 2306 44-45 -0.0415809509618299 2306 46-47 -0.17843793653083395 2306 48-49 -0.15348360041181763 2306 50-51 -0.31413422818121717 2306 52-53 -0.05138833765737161 2306 54-55 -0.09511511572130615 2306 56-57 -0.02713417001661611 2306 58-59 0.04497724185222296 2306 60-61 0.056904587067073464 2306 62-63 0.07416819664303631 2306 64-65 0.09315134221128574 2306 66-67 0.06253749985110346 2306 68-69 0.09281372632576534 2306 70-71 -0.19008531302886666 2306 72-73 -0.10628489153556586 2306 74-75 0.0015487601559165398 2306 76-77 0.0577274240905048 2306 78-79 0.04277615329539941 2306 80-81 0.08745312756658663 2306 82-83 0.19075845209324171 2306 84-85 0.09955773773654641 2306 86-87 0.021884871765816882 2306 88-89 -0.1271206819508528 2306 90-91 -0.04195576233286147 2306 92-93 0.1273693040822259 2306 94-95 0.24982092972675574 2306 96-97 0.3015826526476033 2306 98-99 0.19312316886901115 2306 100-101 0.2725314084808055 2307 1 -0.39759448117467855 2307 2 -0.35383241823165434 2307 3 -0.4842341749874599 2307 4 -0.2702771826788606 2307 5 -0.3344555123478301 2307 6 -0.2850489444770332 2307 7 -0.2220394884958239 2307 8 -0.228598654424907 2307 9 -0.259748352823685 2307 10-11 -0.44372938000147855 2307 12-13 -0.27051989663257814 2307 14-15 -0.44945393692211866 2307 16-17 -0.5282590896174568 2307 18-19 -0.546318010606889 2307 20-21 -0.6737453246398886 2307 22-23 -0.6458691035427933 2307 24-25 -0.46428243315419593 2307 26-27 -0.3493041049661514 2307 28-29 -0.35161369689524946 2307 30-31 -0.27710778758909527 2307 32-33 -0.31380574606647826 2307 34-35 -0.23747238869169252 2307 36-37 -0.4177034529499153 2307 38-39 -0.3805843808125289 2307 40-41 -0.3320749048715186 2307 42-43 -0.4311275584680132 2307 44-45 -0.47244076141227964 2307 46-47 -0.27703779290427377 2307 48-49 -0.34768503852986044 2307 50-51 -0.23452349791065785 2307 52-53 -0.259448570484885 2307 54-55 -0.3457072887828829 2307 56-57 -0.23232870191942112 2307 58-59 -0.25403368181242314 2307 60-61 -0.2635063323786966 2307 62-63 -0.1857303328659654 2307 64-65 -0.2263637196908519 2307 66-67 -0.33016899350865003 2307 68-69 -0.3721990336830032 2307 70-71 -0.34819614796778353 2307 72-73 -0.3352668919489261 2307 74-75 -0.37230154945998706 2307 76-77 -0.6945173103436382 2307 78-79 -0.6170659852616467 2307 80-81 -0.4707403420209708 2307 82-83 -0.33590839504873315 2307 84-85 -0.2508937649832568 2307 86-87 -0.33743007512506296 2307 88-89 -0.38654370329278365 2307 90-91 -0.3472088700997382 2307 92-93 -0.31260709531110464 2307 94-95 -0.1984584677567902 2307 96-97 -0.2901734279959314 2307 98-99 -0.3350253676653523 2307 100-101 -0.24588408973199094 2308 1 -0.5738543342527578 2308 2 -0.48782987780382925 2308 3 -0.4728700689956824 2308 4 -0.7476437532030289 2308 5 -0.4976224873456587 2308 6 -0.8129502539874238 2308 7 -1.4791676362670785 2308 8 -0.6408881769412957 2308 9 -0.70020456532162 2308 10-11 -0.4451983548681824 2308 12-13 -0.4255511779231753 2308 14-15 -1.0161502489243404 2308 16-17 -1.2710403591127744 2308 18-19 -0.7577507088849984 2308 20-21 -0.6546591006085336 2308 22-23 -0.6741884088641896 2308 24-25 -0.6742016675463631 2308 26-27 -0.6149700969564407 2308 28-29 -0.968639575805831 2308 30-31 -1.4824372089517865 2308 32-33 -0.7421250047615331 2308 34-35 -1.0704575644787795 2308 36-37 -1.3766813805936167 2308 38-39 -0.8616324091694381 2308 40-41 -0.9682962223657086 2308 42-43 -0.7595881513101332 2308 44-45 -0.9898124599153277 2308 46-47 -1.1846950851811968 2308 48-49 -1.9257872355995858 2308 50-51 -2.8531774411126136 2308 52-53 -2.029483849065521 2308 54-55 -1.5300465419159153 2308 56-57 -0.7374494094739887 2308 58-59 -0.8628368203970211 2308 60-61 -0.2960200729494389 2308 62-63 -0.2887028973842547 2308 64-65 -0.3041917368433431 2308 66-67 -0.41640703233276355 2308 68-69 -0.7888864677911727 2308 70-71 -0.9938583955904008 2308 72-73 -1.06391943319543 2308 74-75 -1.1187755547476108 2308 76-77 -0.8139886388741324 2308 78-79 -0.5547019854996549 2308 80-81 -0.7587008052843913 2308 82-83 -0.6705032185723283 2308 84-85 -0.9697248694020928 2308 86-87 -0.873292844715646 2308 88-89 -0.976973418073694 2308 90-91 -1.3375877674413665 2308 92-93 -1.000132124851362 2308 94-95 -1.8567286803371452 2308 96-97 -1.2674417683856518 2308 98-99 -1.6791604464902328 2308 100-101 -1.9497021428416623 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 159.0 11 1432.0 12 4221.0 13 7990.0 14 12854.0 15 18458.0 16 25818.0 17 38987.0 18 73873.0 19 187073.0 20 196978.0 21 133675.0 22 118435.0 23 113194.0 24 118348.0 25 128308.0 26 144349.0 27 161157.0 28 179946.0 29 199471.0 30 226790.0 31 264785.0 32 321572.0 33 416079.0 34 592776.0 35 985070.0 36 1585529.0 37 2352153.0 38 1903182.0 39 267782.0 40 173.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 26.11916125773124 33.68225272428724 19.714398562308766 20.484187455672764 2 22.099827672216936 24.36639532167822 35.883313925045854 17.65046308105899 3 21.03034352947113 30.642770201114626 21.95732192718451 26.36956434222973 4 27.471068912746933 22.07992157777968 29.984338560182728 20.46467094929066 5 21.567223697194354 28.511500917665387 21.11319592253431 28.808079462605946 6 21.6769947696876 21.86542552569806 28.7902604470356 27.667319257578736 7 27.66056639795604 22.20679742107549 20.99625457464498 29.136381606323496 8 35.311676937259065 21.9614682572001 21.337292537403698 21.389562268137134 9 21.12528242815022 29.68988419773338 21.650168849318284 27.534664524798114 10-11 32.52673455269448 21.858561355202458 21.397196338836977 24.217507753266094 12-13 28.354782476735796 21.829534432027405 21.52602675709563 28.28965633414117 14-15 28.144012708733396 21.90199578539932 21.62245244196576 28.331539063901534 16-17 28.282770987711466 24.949914745147264 21.467113480878368 25.300200786262906 18-19 25.197261418593996 21.96183001753703 24.656633784816417 28.184274779052554 20-21 24.989374449590922 28.773229883481633 22.068581782602816 24.168813884324628 22-23 20.69165700890246 24.675598374787047 29.35220411297386 25.280540503336635 24-25 20.773707036090137 21.76905349953036 32.307605185528324 25.149634278851174 26-27 20.67431409750873 21.755277575099154 36.60645395521999 20.96395437217212 28-29 20.82466422611394 21.645108842554293 36.75514242318947 20.775084508142296 30-31 20.779797950751338 21.552490342910456 36.79524929402426 20.87246241231395 32-33 20.773278840894356 21.567381494181824 36.84277219228835 20.816567472635466 34-35 20.79324766841916 21.669425981503192 36.74349429068572 20.793832059391935 36-37 20.881688009697587 21.572352707855693 36.60869361950174 20.937265662944977 38-39 20.922903914475867 21.68313635980149 36.1472833366519 21.246676389070743 40-41 21.711987653742085 22.45026690192978 33.807430898881435 22.030314545446704 42-43 21.78902574948029 22.61158226397678 33.81309342035955 21.786298566183383 44-45 21.866818529751544 23.15186464642876 33.318623838815355 21.66269298500434 46-47 21.744740020370187 23.61735028004371 33.08390365531712 21.554006044268984 48-49 21.69094334442117 23.824279398696547 32.780958909446426 21.703818347435853 50-51 21.677719699611302 24.022983963617335 32.55097562692835 21.748320709843018 52-53 21.710853084329713 24.17677609622384 32.3401888732293 21.77218194621714 54-55 21.858807222278884 24.042260890286897 32.009802646124506 22.089129241309717 56-57 21.880035077766415 23.911551267283564 31.992516570014818 22.215897084935204 58-59 22.013772875560843 24.007273243350795 31.684037359667034 22.294916521421328 60-61 22.169987257620452 23.933893607206528 31.341680641666052 22.55443849350697 62-63 22.388447489745023 24.07619860011736 30.996312085262645 22.539041824874975 64-65 22.405668209373523 24.253822925550278 30.61728001122014 22.72322885385606 66-67 22.520424621375994 24.230795346017334 30.303528110082024 22.94525192252465 68-69 22.686664760970135 24.480604543959135 29.93435829379894 22.898372401271786 70-71 22.856887753445523 24.64538730770979 29.706236496897475 22.791488441947212 72-73 22.972047301120888 24.62094295479552 29.54001018428321 22.866999559800384 74-75 22.92511287089514 24.78839287114003 29.334062878209448 22.95243137975538 76-77 23.021929592994614 24.845173753449565 29.197066441274778 22.935830212281044 78-79 23.11558812673158 24.66875227214952 29.200020529975944 23.015639071142957 80-81 23.040438882295664 24.74924299597267 29.16923425010292 23.041083871628746 82-83 23.13992945802675 24.85176983844582 28.997771167508247 23.010529536019185 84-85 23.176572766225227 24.70815814040467 28.932611263897318 23.18265782947278 86-87 23.166756350691035 24.80612342296572 28.964102674463877 23.063017551879373 88-89 23.233829735849433 24.95489762176413 28.783468835656194 23.02780380673024 90-91 23.40280492268625 24.747417077573846 28.66100533383151 23.18877266590839 92-93 23.387003416687243 24.799334323876423 28.626628825917027 23.187033433519304 94-95 23.44066149114155 24.916268676002005 28.47518967613437 23.16788015672207 96-97 23.341897510853567 24.924043229948328 28.38631758192884 23.34774167726927 98-99 23.419876639067862 24.9003395442661 28.405313511333148 23.274470305332894 100-101 23.424151457071666 25.109153120938167 28.013723710039667 23.452971711950504 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 50252.0 1 29045.0 2 5420.5 3 2300.0 4 1391.0 5 1106.5 6 991.0 7 926.0 8 918.5 9 913.0 10 1070.5 11 1212.0 12 2102.0 13 3852.0 14 5461.5 15 8992.5 16 240257.5 17 265383.5 18 54585.0 19 40434.5 20 29384.0 21 23458.5 22 22055.5 23 23118.0 24 25208.5 25 28710.0 26 33430.0 27 39150.5 28 46268.0 29 54746.0 30 64754.5 31 75289.5 32 86895.5 33 101852.0 34 117226.5 35 133206.0 36 153918.0 37 175205.0 38 196521.0 39 217454.0 40 235444.0 41 250341.5 42 266647.5 43 285471.0 44 307685.0 45 333937.0 46 358864.0 47 384032.0 48 408833.0 49 428258.0 50 431852.0 51 430962.5 52 437900.0 53 446485.5 54 444878.5 55 429412.5 56 404808.5 57 371481.0 58 333654.5 59 295046.0 60 255690.0 61 216451.5 62 178134.5 63 136816.0 64 99395.0 65 70971.5 66 49649.0 67 36094.0 68 26704.5 69 19863.0 70 15352.5 71 11914.0 72 9877.0 73 7687.5 74 5732.0 75 4848.5 76 3939.0 77 2507.0 78 1452.0 79 852.5 80 480.0 81 317.0 82 241.0 83 155.0 84 90.0 85 43.5 86 16.5 87 3.0 88 1.0 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 1.5 96 1.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 9.275906075143373E-6 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.710362430057349E-5 28-29 0.0 30-31 0.003019307427459168 32-33 0.006043252807955908 34-35 0.0015212485963235131 36-37 0.0019108366514795348 38-39 0.003362515952239473 40-41 0.0032233773611123223 42-43 0.0024534771568754223 44-45 0.006794601200042521 46-47 0.001465593159872653 48-49 3.524844308554482E-4 50-51 0.0029590140379707358 52-53 0.0019711300409679666 54-55 5.519164114710307E-4 56-57 0.0 58-59 7.420724860114698E-5 60-61 5.287266462831722E-4 62-63 0.0 64-65 0.0 66-67 1.8551812150286745E-5 68-69 0.006098908244406768 70-71 0.00638182337969864 72-73 0.002611167560152859 74-75 0.003534120214629625 76-77 0.03280887978778211 78-79 0.05685202833455373 80-81 0.04866140327020213 82-83 7.9772792246233E-4 84-85 0.0011316605411674915 86-87 0.0016418353753003768 88-89 0.003909794410672932 90-91 0.0 92-93 0.0 94-95 4.637953037571687E-5 96-97 0.005913390122903901 98-99 0.0012847129914073572 100-101 5.101748341328855E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0780618E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.62205671229783 #Duplication Level Percentage of deduplicated Percentage of total 1 76.30575451082564 34.812254597675626 2 13.054897052593489 11.911825074132597 3 4.046227462555581 5.5379165630270295 4 1.7642768826522992 3.2195975998623685 5 0.9461266351740552 2.158212150346313 6 0.6061429395299507 1.6592092537796594 7 0.4137146058947486 1.3212157848985318 8 0.3053601221662374 1.1144925448913818 9 0.25680973976506893 1.054456966064918 >10 2.0914745726337616 19.521907148470014 >50 0.1793817095220737 5.480647634758358 >100 0.02755221346510086 1.837012545446268 >500 9.98783453537067E-4 0.32152044984918154 >1k 8.551802933183081E-4 0.7439149492513224 >5k 3.0542105370040166E-4 0.9513348789759091 >10k+ 1.2216842148016067E-4 8.354481858570544 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 725724 6.73174766047735 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT 74333 0.6895059262836323 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 46866 0.43472461411766933 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26875 0.24928997576947812 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12843 0.11913046172306634 No Hit GTCGACGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12726 0.11804518071227457 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.637953037571687E-5 0.0 0.0 0.0 0.0 2 4.637953037571687E-5 0.0 0.0 0.0 0.0 3 4.637953037571687E-5 0.0 0.0 0.0 9.275906075143373E-6 4 4.637953037571687E-5 0.0 0.0 0.0 3.710362430057349E-5 5 5.5655436450860236E-5 0.0 0.0 0.0 3.710362430057349E-5 6 5.5655436450860236E-5 0.0 0.0 0.0 4.637953037571687E-5 7 5.5655436450860236E-5 0.0 0.0 0.0 5.5655436450860236E-5 8 5.5655436450860236E-5 0.0 0.0 9.275906075143373E-6 6.49313425260036E-5 9 8.348315467629036E-5 0.0 0.0 9.275906075143373E-6 7.420724860114698E-5 10-11 9.275906075143373E-5 0.0 0.0 9.275906075143373E-6 1.0203496682657711E-4 12-13 1.0203496682657711E-4 0.0 0.0 9.275906075143373E-6 1.7160426239015242E-4 14-15 1.2522473201443554E-4 0.0 0.0 9.275906075143373E-6 2.1334583972829757E-4 16-17 1.7160426239015242E-4 0.0 4.637953037571686E-6 9.275906075143373E-6 2.597253701040144E-4 18-19 2.4581151099129937E-4 0.0 9.275906075143373E-6 9.275906075143373E-6 3.0610490047973133E-4 20-21 3.8495010211845E-4 0.0 1.3913859112715059E-5 9.275906075143373E-6 3.1538080655487473E-4 22-23 5.33364599320744E-4 0.0 1.8551812150286745E-5 9.275906075143373E-6 3.478464778178765E-4 24-25 7.09606814748468E-4 0.0 1.8551812150286745E-5 9.275906075143373E-6 3.9886396123116504E-4 26-27 0.0010017978561154843 0.0 1.8551812150286745E-5 9.275906075143373E-6 4.7307120983231205E-4 28-29 0.0013589202400085042 0.0 1.8551812150286745E-5 9.275906075143373E-6 5.008989280577421E-4 30-31 0.0016603871874506639 0.0 1.8551812150286745E-5 9.275906075143373E-6 5.33364599320744E-4 32-33 0.001920112557554678 0.0 1.8551812150286745E-5 9.275906075143373E-6 6.029338948843193E-4 34-35 0.0022308554110719815 0.0 1.8551812150286745E-5 9.275906075143373E-6 6.400375191848928E-4 36-37 0.0025694259828147143 0.0 1.8551812150286745E-5 9.275906075143373E-6 6.864170495606097E-4 38-39 0.0028523411181065874 0.0 1.8551812150286745E-5 9.275906075143373E-6 7.327965799363265E-4 40-41 0.003153808065548747 0.0 1.8551812150286745E-5 9.275906075143373E-6 7.559863451241848E-4 42-43 0.003445999106915763 0.0 1.8551812150286745E-5 9.275906075143373E-6 7.930899694247584E-4 44-45 0.003817035349921498 0.0 1.8551812150286745E-5 9.275906075143373E-6 8.348315467629036E-4 46-47 0.004090674579138228 0.0 1.8551812150286745E-5 9.275906075143373E-6 8.719351710634771E-4 48-49 0.0042993824658289536 0.0 1.8551812150286745E-5 9.275906075143373E-6 9.322285605519089E-4 50-51 0.004545193976820253 0.0 1.8551812150286745E-5 9.275906075143373E-6 9.786080909276258E-4 52-53 0.00503217904576528 0.0 1.8551812150286745E-5 9.275906075143373E-6 0.001034263527378486 54-55 0.005704682236213175 0.0 1.8551812150286745E-5 9.275906075143373E-6 0.0010574532925663445 56-57 0.006344719755398067 0.0 1.8551812150286745E-5 9.275906075143373E-6 0.0011409364472426348 58-59 0.007091430194447109 0.0 1.8551812150286745E-5 9.275906075143373E-6 0.0011873159776183517 60-61 0.008199900970426742 0.0 1.8551812150286745E-5 9.275906075143373E-6 0.0012151436958437818 62-63 0.009424320572345667 0.0 1.8551812150286745E-5 9.275906075143373E-6 0.0012522473201443552 64-65 0.010811068530579601 9.275906075143373E-6 1.8551812150286745E-5 9.275906075143373E-6 0.0013125407096327873 66-67 0.012805388336735425 9.275906075143373E-6 1.8551812150286745E-5 9.275906075143373E-6 0.0013496443339333607 68-69 0.015189296198047273 9.275906075143373E-6 1.8551812150286745E-5 9.275906075143373E-6 0.001391385911271506 70-71 0.018046275269191434 9.275906075143373E-6 1.8551812150286745E-5 9.275906075143373E-6 0.0014331274886096512 72-73 0.02184012085392507 9.275906075143373E-6 1.8551812150286745E-5 9.275906075143373E-6 0.001507334737210798 74-75 0.02670533359033777 9.275906075143373E-6 1.8551812150286745E-5 9.275906075143373E-6 0.0015583522206240865 76-77 0.03264191347842953 9.275906075143373E-6 1.8551812150286745E-5 9.275906075143373E-6 0.0015815419858119452 78-79 0.0400997419628448 9.275906075143373E-6 1.8551812150286745E-5 1.3913859112715059E-5 0.0016186456101125186 80-81 0.0491901299164853 9.275906075143373E-6 1.8551812150286745E-5 1.8551812150286745E-5 0.0016325594692252338 82-83 0.06051601123423536 9.275906075143373E-6 1.8551812150286745E-5 1.8551812150286745E-5 0.0016650251404882353 84-85 0.07437421491049956 9.275906075143373E-6 1.8551812150286745E-5 1.8551812150286745E-5 0.0017206805769390957 86-87 0.09152072729040209 9.275906075143373E-6 1.8551812150286745E-5 1.8551812150286745E-5 0.0017485082951645258 88-89 0.11247499911415097 9.275906075143373E-6 1.8551812150286745E-5 3.710362430057349E-5 0.001818077590728101 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 63805 0.0 94.039314 1 TATCTCG 93060 0.0 92.32064 2 ATATCTC 93300 0.0 92.19006 1 TCGACGG 65760 0.0 91.33748 2 ATCTCGA 97195 0.0 88.265976 3 ACGGCGC 68155 0.0 88.01634 5 GACGGCG 68410 0.0 87.75074 4 CGACGGC 68520 0.0 87.56135 3 CGGCGCG 68935 0.0 87.054886 6 TCTCGAG 97650 0.0 86.653404 4 CTCGAGG 100350 0.0 84.29826 5 TCGAGGG 100385 0.0 84.27832 6 CGAGGGC 101780 0.0 83.1512 7 AGGGCGC 102430 0.0 82.744095 9 GAGGGCG 104485 0.0 81.225784 8 CTCGACG 2610 0.0 43.307163 5 GGGCGCG 103085 0.0 41.203613 10-11 GATCCTT 101935 0.0 39.87006 20-21 GGATCCT 103315 0.0 39.85695 18-19 TCCATAT 74610 0.0 39.564198 18-19 >>END_MODULE