FastQCFastQC Report
Tue 31 May 2016
SRR1051265_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051265_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10780618
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3392143.146517203373684No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT426540.3956544977291654No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT373310.3462788496911773No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165340.15336783104642052No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112400.10426118428461151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG106600.090.948131
TCGACGG110000.088.394312
ACGGCGC135250.072.1714865
GACGGCG139750.069.881534
CGACGGC140150.069.749863
CGGCGCG144200.067.593236
ATCTCGA74000.059.1491741
TCTCGAG97100.045.5438352
CGCGCCG275750.042.5128979
GGCGCGC301050.039.0190437
GCGCGCC305850.038.4222078
TCGAGGG124850.035.2681164
CTCGAGG129300.034.1645363
AATTCGA42450.034.034291
CGAGGGC143650.030.7847185
AGGGCGC149200.029.7032497
GGGCGCG148250.029.7013478
TATCTCG24450.026.6158832
CCATATT190650.026.37212818-19
TCCATAT190550.026.08683618-19
Produced by FastQC (version 0.11.5)