FastQCFastQC Report
Tue 31 May 2016
SRR1051264_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051264_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4619548
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT127465527.59263460407815No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT72050115.596785659549376No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTA276560.5986732901140978No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246440.5334721059289784No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAA239910.5193365238330676No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168130.3639533564755686No Hit
CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154450.33434006963451834No Hit
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141640.30661008393028927No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA128510.27818738976194207No Hit
ATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT128160.27742973987931285No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA126940.27478878885986247No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTAAA125370.2713901879577829No Hit
GGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124520.2695501810999691No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT105600.2285937931589844No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA103750.22458907235080144No Hit
ATATCTCGAGGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT91130.1972703822971425No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTC85080.184173862897409No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC81890.17726842539573134No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTAAA81140.17564488993295446No Hit
TGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77210.16713756410800365No Hit
ATATGGATCTGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT77020.1667262684574335No Hit
TATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT75560.1635657860898945No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75200.1627864890677616No Hit
ATATGGATTCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT73200.15845706116702327No Hit
ATATGGACCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT70100.15174644792087882No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTG68970.14930032115696168No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC60230.13038072123073513No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG51240.11091994281691628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGGA1530350.092.493971
TATGGAT1534200.091.645942
ATGGATC1539300.090.684053
ATATCTC898600.090.1258851
TGGATCC1538950.089.8446964
TATCTCG899600.089.82452
GGATCCG1541150.089.516115
ATCTCGA914200.088.2747043
TCTCGAG917950.087.886324
GATCCGG1580400.087.559486
CTCGAGG927100.087.100915
TCGAGGG928150.087.057726
CGAGGGC932800.086.6644747
ATCCGGC1600900.086.411557
AGGGCGC942800.086.1785059
GAGGGCG943650.085.9599468
TCCGGCG1619450.085.641718
CCGGCGC1656050.084.210739
ATCGGCG4500.066.496397
TATCGAT3200.065.311782
Produced by FastQC (version 0.11.5)