##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051264_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4619548 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04532499716422 33.0 31.0 34.0 30.0 34.0 2 32.36372497915381 34.0 31.0 34.0 31.0 34.0 3 32.3610602162809 34.0 31.0 34.0 31.0 34.0 4 35.812690765416875 37.0 35.0 37.0 35.0 37.0 5 35.79551722376302 37.0 35.0 37.0 35.0 37.0 6 35.7256229397335 37.0 36.0 37.0 33.0 37.0 7 35.6564692043464 37.0 36.0 37.0 33.0 37.0 8 35.71279246367826 37.0 35.0 37.0 33.0 37.0 9 37.352142244219564 39.0 37.0 39.0 34.0 39.0 10-11 37.330588836829925 39.0 37.0 39.0 34.0 39.0 12-13 37.374037676413366 39.0 37.5 39.0 34.5 39.0 14-15 38.94488259457419 41.0 39.0 41.0 35.0 41.0 16-17 38.91185176558399 41.0 39.0 41.0 35.5 41.0 18-19 38.932233629783696 41.0 39.0 41.0 35.0 41.0 20-21 38.16543501658604 40.0 38.0 41.0 33.0 41.0 22-23 38.028731274141975 40.0 38.0 41.0 32.5 41.0 24-25 38.314234531170584 40.0 38.0 41.0 33.5 41.0 26-27 38.66069916364112 40.5 38.0 41.0 34.0 41.0 28-29 38.711786629341226 41.0 38.0 41.0 34.5 41.0 30-31 38.23896558710939 40.0 38.0 40.5 34.5 41.0 32-33 36.07606988822283 36.0 35.0 39.5 33.0 41.0 34-35 35.83442925584927 35.0 35.0 39.0 33.0 41.0 36-37 35.68911330718936 35.0 35.0 39.0 33.0 41.0 38-39 35.60879246194649 35.0 35.0 39.0 33.0 41.0 40-41 35.53719541392361 35.0 35.0 39.0 33.0 41.0 42-43 35.46029081200152 35.0 35.0 39.0 33.0 41.0 44-45 35.317767236101886 35.0 35.0 38.5 33.0 41.0 46-47 34.80511101951966 35.0 35.0 38.0 30.5 41.0 48-49 33.54020274277916 35.0 34.0 38.0 21.5 41.0 50-51 32.32981257040732 35.0 32.0 37.5 20.0 40.0 52-53 30.16396723229199 35.0 28.5 38.0 7.0 40.0 54-55 28.37012896066888 35.0 21.5 38.0 2.0 41.0 56-57 26.89529549211308 35.0 12.5 37.5 2.0 41.0 58-59 25.964007625854304 35.0 7.0 37.0 2.0 41.0 60-61 25.278348985658337 35.0 2.0 36.5 2.0 40.0 62-63 24.665608843116253 34.0 2.0 36.0 2.0 40.0 64-65 24.075325226623903 33.5 2.0 35.5 2.0 40.0 66-67 23.363902702169128 33.0 2.0 35.0 2.0 39.0 68-69 22.62829252991851 33.0 2.0 35.0 2.0 39.0 70-71 22.05579929032018 33.0 2.0 35.0 2.0 39.0 72-73 21.38508367052361 31.5 2.0 35.0 2.0 37.5 74-75 20.687987006520984 30.5 2.0 35.0 2.0 37.0 76-77 20.03141735944729 29.0 2.0 35.0 2.0 36.5 78-79 19.510692604557846 28.0 2.0 35.0 2.0 36.0 80-81 19.059522598314814 26.5 2.0 35.0 2.0 36.0 82-83 18.659232028761256 24.5 2.0 35.0 2.0 35.0 84-85 18.280032158990444 21.5 2.0 35.0 2.0 35.0 86-87 17.95388325870843 17.0 2.0 35.0 2.0 35.0 88-89 17.64166873036063 6.5 2.0 35.0 2.0 35.0 90-91 17.34769375705156 2.0 2.0 35.0 2.0 35.0 92-93 17.05810871539813 2.0 2.0 35.0 2.0 35.0 94-95 16.768369113168646 2.0 2.0 35.0 2.0 35.0 96-97 16.468882020492046 2.0 2.0 35.0 2.0 35.0 98-99 16.105348077344363 2.0 2.0 35.0 2.0 35.0 100-101 15.678416914382101 2.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4947152384978324 1101 2 0.4097897280670537 1101 3 0.41880476748123385 1101 4 0.34366971580323735 1101 5 0.3461214215297943 1101 6 0.3871871596383869 1101 7 0.48067515451079146 1101 8 0.4102600424788889 1101 9 0.5126208373143513 1101 10-11 0.4437114885261053 1101 12-13 0.41676453984241135 1101 14-15 0.5642859728902394 1101 16-17 0.5819529750931522 1101 18-19 0.5735191342276238 1101 20-21 0.5863032014739886 1101 22-23 0.596626408503397 1101 24-25 0.5814569128842635 1101 26-27 0.5001589739642753 1101 28-29 0.48669695704619187 1101 30-31 0.41847014049742626 1101 32-33 0.44170795533616314 1101 34-35 0.47127052113125245 1101 36-37 0.5612364197799451 1101 38-39 0.5967895241454997 1101 40-41 0.5522236126270954 1101 42-43 0.47499512365683216 1101 44-45 0.3710072861927429 1101 46-47 0.37657107469633644 1101 48-49 0.26192638954917413 1101 50-51 0.16433955602256134 1101 52-53 0.04458581635973147 1101 54-55 -0.134008409481158 1101 56-57 -0.031144702243672384 1101 58-59 -0.04890079197209829 1101 60-61 -0.03859954796973497 1101 62-63 -0.10425796970708134 1101 64-65 -0.0873072081396451 1101 66-67 0.012105828895240478 1101 68-69 -0.029382197928203624 1101 70-71 -0.10551211540166605 1101 72-73 -0.04165289870943667 1101 74-75 -0.14883341619108492 1101 76-77 -0.11507287561289559 1101 78-79 -0.1551297580124178 1101 80-81 -0.2850447384116066 1101 82-83 -0.2740183021057625 1101 84-85 -0.14771947509123962 1101 86-87 -0.07586239040549003 1101 88-89 -0.0746133688359727 1101 90-91 0.023791561825774465 1101 92-93 0.06910502262603657 1101 94-95 0.15123940594565966 1101 96-97 0.1618341342217562 1101 98-99 0.21624337157749096 1101 100-101 0.22605219872538918 1102 1 0.5126639591188109 1102 2 0.3756401699233862 1102 3 0.34758512836042854 1102 4 0.31709108877512193 1102 5 0.3425911164139137 1102 6 0.3865070168340523 1102 7 0.4283039982415673 1102 8 0.30831205544585316 1102 9 0.4331051368301999 1102 10-11 0.44536780006400534 1102 12-13 0.4328064448910922 1102 14-15 0.5313209660664953 1102 16-17 0.543953543321841 1102 18-19 0.5521994017194629 1102 20-21 0.5590399489307316 1102 22-23 0.6527429732007519 1102 24-25 0.6974763028331523 1102 26-27 0.5680694171786342 1102 28-29 0.5730819475354991 1102 30-31 0.48292096646928684 1102 32-33 0.4620498756728253 1102 34-35 0.4572572464817455 1102 36-37 0.4522643255432399 1102 38-39 0.5262362790677244 1102 40-41 0.4348129322098444 1102 42-43 0.4752281578123032 1102 44-45 0.48306939783199 1102 46-47 0.45794231014452436 1102 48-49 0.5266320392585797 1102 50-51 0.42102558265539614 1102 52-53 0.24233127069723892 1102 54-55 0.028596967960023534 1102 56-57 0.0839958759680357 1102 58-59 -0.04495404235430556 1102 60-61 -0.11716258221900588 1102 62-63 -0.1633517224436396 1102 64-65 -0.0840605764458715 1102 66-67 0.005381655796387719 1102 68-69 -0.03567273055254461 1102 70-71 -0.15833626596537798 1102 72-73 -0.18995935060895874 1102 74-75 -0.2294372062048211 1102 76-77 -0.20413499978502614 1102 78-79 -0.15353816185391622 1102 80-81 -0.10047813317210341 1102 82-83 -0.12231966068380373 1102 84-85 -0.040456349212956155 1102 86-87 -0.05780734736652349 1102 88-89 -0.013633463752931618 1102 90-91 -0.01564685351738504 1102 92-93 0.014662372958344605 1102 94-95 0.10802585999795511 1102 96-97 0.19019915156884792 1102 98-99 0.31792872921083415 1102 100-101 0.3388857775455971 1103 1 0.35747191185023297 1103 2 0.16859361631625802 1103 3 0.2032848898053956 1103 4 0.16532615267877304 1103 5 0.2057159906095123 1103 6 0.21769360394251436 1103 7 0.37836509433918764 1103 8 0.28186749707161596 1103 9 0.33532563432805773 1103 10-11 0.3136318400842555 1103 12-13 0.3352580986335383 1103 14-15 0.31739657999197135 1103 16-17 0.2881951209040423 1103 18-19 0.29889443291895645 1103 20-21 0.36539062981901793 1103 22-23 0.48986782074076984 1103 24-25 0.490533182864084 1103 26-27 0.4149027958513827 1103 28-29 0.39275563209479003 1103 30-31 0.415928671926558 1103 32-33 0.3307786383952589 1103 34-35 0.39082311866248176 1103 36-37 0.37322137068932904 1103 38-39 0.320850194080748 1103 40-41 0.3923905547707349 1103 42-43 0.3458930315794291 1103 44-45 0.3342128477324451 1103 46-47 0.44410116049657944 1103 48-49 0.41628101508619864 1103 50-51 0.3148920576011349 1103 52-53 0.23407880717303797 1103 54-55 0.10621571734968072 1103 56-57 0.32557685862745345 1103 58-59 0.2678251873640356 1103 60-61 0.19697435724142665 1103 62-63 0.19052731301276893 1103 64-65 0.16936208174880107 1103 66-67 0.24016099238618338 1103 68-69 0.19954837877820708 1103 70-71 0.19435645267519774 1103 72-73 0.16930535879034636 1103 74-75 0.1687260744550798 1103 76-77 0.2575536116532575 1103 78-79 0.2758279732953852 1103 80-81 0.2696702512502718 1103 82-83 0.23591391972439268 1103 84-85 0.27347364200814184 1103 86-87 0.24153394203641199 1103 88-89 0.29921333912128745 1103 90-91 0.29544416833322273 1103 92-93 0.3757011486149686 1103 94-95 0.36868940223136093 1103 96-97 0.4747309835698985 1103 98-99 0.6006319132828466 1103 100-101 0.6967785525863999 1104 1 0.2627642727776802 1104 2 0.23951786475142 1104 3 0.21580582782884505 1104 4 0.2082694877753113 1104 5 0.16775480309298274 1104 6 0.26202380759234245 1104 7 0.31624473376438544 1104 8 0.23321634658232426 1104 9 0.3550244573907406 1104 10-11 0.2870441318060273 1104 12-13 0.24913504917572737 1104 14-15 0.2627926943356229 1104 16-17 0.24699487625976957 1104 18-19 0.3145081374063068 1104 20-21 0.36008669787861436 1104 22-23 0.4273890342645288 1104 24-25 0.47959416761239027 1104 26-27 0.43958927650024293 1104 28-29 0.36112130916434637 1104 30-31 0.32126924540799706 1104 32-33 0.25455486602463395 1104 34-35 0.23156465529300618 1104 36-37 0.25875209448373937 1104 38-39 0.26062582662945744 1104 40-41 0.2623477971228496 1104 42-43 0.18005314133874606 1104 44-45 0.24026007950534023 1104 46-47 0.24583295543619244 1104 48-49 0.4421493920587807 1104 50-51 0.406153187813608 1104 52-53 0.36365569379915286 1104 54-55 0.21656901894538194 1104 56-57 0.33107915079002126 1104 58-59 0.31646889934499 1104 60-61 0.1947058877007386 1104 62-63 0.09798180036179716 1104 64-65 0.16988966483969747 1104 66-67 0.1674222989188472 1104 68-69 0.10299764264138034 1104 70-71 0.13239946378336853 1104 72-73 0.19060759364884916 1104 74-75 0.22003910892567902 1104 76-77 0.16216218081485678 1104 78-79 0.19576868562898042 1104 80-81 0.24537498103956779 1104 82-83 0.20049201855186638 1104 84-85 0.17520350844559118 1104 86-87 0.17430993824552488 1104 88-89 0.1573127726926522 1104 90-91 0.23105010371186907 1104 92-93 0.2581076989103046 1104 94-95 0.28771435153930724 1104 96-97 0.36605189734932253 1104 98-99 0.481172499988304 1104 100-101 0.5475436169432708 1105 1 0.10570710939480676 1105 2 0.03377471763172224 1105 3 0.048334156165040554 1105 4 0.03167683680850786 1105 5 0.021469587880510232 1105 6 -0.011151032281226492 1105 7 0.1101621538590365 1105 8 0.05857765698998918 1105 9 0.04507022010169237 1105 10-11 0.04559785930790383 1105 12-13 0.05143669341491375 1105 14-15 0.029905217341209323 1105 16-17 0.02526463330424633 1105 18-19 0.004590245090490441 1105 20-21 0.015505786646507147 1105 22-23 -0.24127439148224994 1105 24-25 -0.6040958655924413 1105 26-27 -0.35538518077058256 1105 28-29 -0.6393947132112885 1105 30-31 -0.37195724906501226 1105 32-33 -0.1511966758140062 1105 34-35 0.05663139370105341 1105 36-37 0.06254602979881696 1105 38-39 0.09804645297527514 1105 40-41 0.08648170364270413 1105 42-43 0.0981570798668443 1105 44-45 0.023500514795564698 1105 46-47 -0.12823665697738562 1105 48-49 -0.4024670120046636 1105 50-51 -0.491498336022417 1105 52-53 -0.13418800036873435 1105 54-55 -0.09683780579630152 1105 56-57 0.2865747114446755 1105 58-59 0.44522186681703246 1105 60-61 0.3884370744258412 1105 62-63 0.40573409737756094 1105 64-65 0.358451007574935 1105 66-67 0.4053117367366177 1105 68-69 0.2248035064630045 1105 70-71 0.09476290131661358 1105 72-73 -0.07815783236200247 1105 74-75 -0.25282722979193295 1105 76-77 -0.2901732564568249 1105 78-79 -0.11248192939322266 1105 80-81 -0.18889447614066057 1105 82-83 -0.015031133111993 1105 84-85 0.12598018196706917 1105 86-87 0.2336333158256565 1105 88-89 0.15383227759283002 1105 90-91 0.1881609217609146 1105 92-93 0.1593491844429984 1105 94-95 0.20191804220175058 1105 96-97 0.1993474112796889 1105 98-99 0.2245213474619021 1105 100-101 -0.03355501350323742 1106 1 -0.06851938222426313 1106 2 -0.15959413762318064 1106 3 -0.18749129441842172 1106 4 -0.24384312093704352 1106 5 -0.1770443344152497 1106 6 -0.24587692853958032 1106 7 -0.04459766460594494 1106 8 -0.12076720489894655 1106 9 -0.14976415591110026 1106 10-11 -0.17671759187990688 1106 12-13 -0.1808819177922416 1106 14-15 -0.18854735819785873 1106 16-17 -0.17950441923633065 1106 18-19 -0.1395317538741523 1106 20-21 -0.16806463431343843 1106 22-23 -0.06931046202173974 1106 24-25 -0.019316710465844267 1106 26-27 -0.009562159398576853 1106 28-29 -0.059911324503609364 1106 30-31 -0.07863122655082577 1106 32-33 -0.09076977865281322 1106 34-35 -0.1531024794075364 1106 36-37 -0.16091440241174126 1106 38-39 -0.17008600632076565 1106 40-41 -0.13604980966170643 1106 42-43 -0.18996559270448898 1106 44-45 -0.14899140064225236 1106 46-47 4.734789049649635E-4 1106 48-49 0.1549284603203276 1106 50-51 0.18680039753490973 1106 52-53 0.3415777894218657 1106 54-55 0.4811488492147973 1106 56-57 0.6351972429763677 1106 58-59 0.6797546874411147 1106 60-61 0.6277763252333166 1106 62-63 0.6291660425757968 1106 64-65 0.585434515731194 1106 66-67 0.6146850303621498 1106 68-69 0.6588220854036209 1106 70-71 0.6941031167991447 1106 72-73 0.6375793635426596 1106 74-75 0.6711013218605331 1106 76-77 0.6754815477823399 1106 78-79 0.6130988408490801 1106 80-81 0.6306579328064501 1106 82-83 0.5479948753517725 1106 84-85 0.5380918103395338 1106 86-87 0.47604317666094786 1106 88-89 0.3909180634801608 1106 90-91 0.386687305434382 1106 92-93 0.37539901273288834 1106 94-95 0.3394107829354205 1106 96-97 0.28253503645663613 1106 98-99 0.32570630657468413 1106 100-101 0.3787225448200413 1107 1 -0.42256866173216423 1107 2 -0.5355554550566701 1107 3 -0.530680731122743 1107 4 -0.49216039752705143 1107 5 -0.41573016669263296 1107 6 -0.5509254039862483 1107 7 -0.292836597182891 1107 8 -0.30703058694267327 1107 9 -0.30071716542716587 1107 10-11 -0.3556955020570527 1107 12-13 -0.35866565626589164 1107 14-15 -0.48387351776796095 1107 16-17 -0.5166682963152098 1107 18-19 -0.6199229479341994 1107 20-21 -0.6001825789119408 1107 22-23 -0.8892954224818013 1107 24-25 -0.565679318608602 1107 26-27 -0.6315359320825138 1107 28-29 -0.4560091639976349 1107 30-31 -0.6333688846025822 1107 32-33 -0.4872846615806523 1107 34-35 -0.572178352704114 1107 36-37 -0.5787509946984315 1107 38-39 -0.6485261368312294 1107 40-41 -0.6579532073734171 1107 42-43 -0.48512234435539625 1107 44-45 -0.40561929250589657 1107 46-47 -0.31950924431167493 1107 48-49 -0.1370593321862188 1107 50-51 -0.12593539817873278 1107 52-53 0.3316032724343678 1107 54-55 0.5866102519722958 1107 56-57 0.9705061719252335 1107 58-59 0.8839248646481046 1107 60-61 0.9236970145286243 1107 62-63 0.9457722491729577 1107 64-65 0.9339009212990668 1107 66-67 0.9126589877755826 1107 68-69 0.9437811778124754 1107 70-71 0.8365948564864176 1107 72-73 0.7972827990081086 1107 74-75 0.7696916945039973 1107 76-77 0.8286818227668888 1107 78-79 0.8894445106294988 1107 80-81 0.8901178509049146 1107 82-83 0.7220412834943986 1107 84-85 0.7576427627841724 1107 86-87 0.671743099633975 1107 88-89 0.5200535358281009 1107 90-91 0.4702882230362313 1107 92-93 0.38087891273560004 1107 94-95 0.36504642909490315 1107 96-97 0.24951102041780615 1107 98-99 0.33925983095841517 1107 100-101 0.379314882856546 1108 1 -0.915740047503597 1108 2 -0.7378669968503182 1108 3 -0.6248091696005496 1108 4 -0.46904613323403055 1108 5 -0.5468493646102175 1108 6 -0.5935492308672821 1108 7 -0.6838906378129721 1108 8 -0.6026231230430312 1108 9 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-0.05442429200240895 2305 20-21 -0.15314458867051428 2305 22-23 -0.17432782615894382 2305 24-25 -0.3982464297118824 2305 26-27 -0.5580925910564503 2305 28-29 -0.35499040952456085 2305 30-31 -0.17034314175338494 2305 32-33 -0.038124715517177776 2305 34-35 -0.03362502233535736 2305 36-37 -0.08090227945211126 2305 38-39 0.0327313153322919 2305 40-41 0.07134071926592611 2305 42-43 -0.010400565668895467 2305 44-45 -0.06964470218393615 2305 46-47 -0.1710849634047733 2305 48-49 -0.4619773576502766 2305 50-51 -0.5562498762841805 2305 52-53 -0.4807346860785273 2305 54-55 -0.31227493601971545 2305 56-57 0.11808497154411413 2305 58-59 0.21042425872328963 2305 60-61 0.26212172325355354 2305 62-63 0.21987516528369966 2305 64-65 0.11819833418353554 2305 66-67 0.1787496546066656 2305 68-69 0.050409956762003105 2305 70-71 -0.0858308025607748 2305 72-73 -0.2913987873144208 2305 74-75 -0.49834314085467213 2305 76-77 -0.42409136608138454 2305 78-79 -0.4174017418319771 2305 80-81 -0.4059459174570996 2305 82-83 -0.20306010603734492 2305 84-85 -0.009353709447193381 2305 86-87 -0.0327387130467649 2305 88-89 -0.09540337933727372 2305 90-91 -0.049161537894885754 2305 92-93 -0.06563110758457569 2305 94-95 0.005232671693761404 2305 96-97 -0.07515977596428414 2305 98-99 -0.13910582786779102 2305 100-101 -0.39749532416609235 2306 1 -0.41749735952882716 2306 2 -0.26601772539943624 2306 3 -0.2827028590460614 2306 4 -0.20582706056106304 2306 5 -0.21580092860276068 2306 6 -0.33024505971509655 2306 7 -0.18249722128908274 2306 8 -0.22455730344422165 2306 9 -0.4228859599332466 2306 10-11 -0.28841732235093076 2306 12-13 -0.22244471026466073 2306 14-15 -0.35668718656453535 2306 16-17 -0.33305691087908684 2306 18-19 -0.4069564308407507 2306 20-21 -0.38344856713968056 2306 22-23 -0.22033785952037022 2306 24-25 -0.0639540353729302 2306 26-27 -0.09803664021965375 2306 28-29 -0.052811470481714196 2306 30-31 -0.13338967322805928 2306 32-33 -0.17849099433895077 2306 34-35 -0.25074095135175156 2306 36-37 -0.296331099690299 2306 38-39 -0.24569574100966207 2306 40-41 -0.2042289610939676 2306 42-43 -0.2796050911201249 2306 44-45 -0.2690312886421893 2306 46-47 -0.1822608058664983 2306 48-49 -0.07228541918478015 2306 50-51 0.06494092114591155 2306 52-53 0.18621612548797017 2306 54-55 0.085505970163954 2306 56-57 0.36936799803800113 2306 58-59 0.3555843656504827 2306 60-61 0.22628005832414644 2306 62-63 0.31550581976970804 2306 64-65 0.3300370871221112 2306 66-67 0.34006528028817584 2306 68-69 0.2752673949250024 2306 70-71 0.2517943741442572 2306 72-73 0.3102157319667782 2306 74-75 0.25996556291589457 2306 76-77 0.27911771525069184 2306 78-79 0.21463503113907834 2306 80-81 0.20081362461476715 2306 82-83 0.1210290932823277 2306 84-85 0.047363969377098414 2306 86-87 -0.04770541544995055 2306 88-89 -0.1437796117666501 2306 90-91 -0.2216232297753713 2306 92-93 -0.269610973574121 2306 94-95 -0.21299667552044355 2306 96-97 -0.23395690431597416 2306 98-99 -0.07967313964426381 2306 100-101 0.011998035539571461 2307 1 -0.45396107705336064 2307 2 -0.41960139369425775 2307 3 -0.5318940270224601 2307 4 -0.28850435565201593 2307 5 -0.38723444035183263 2307 6 -0.3964237736407057 2307 7 -0.2309962260313725 2307 8 -0.22932180354937515 2307 9 -0.408082056804993 2307 10-11 -0.4755938080544695 2307 12-13 -0.5591052577748883 2307 14-15 -0.9014315741700258 2307 16-17 -0.7126225815968894 2307 18-19 -0.7199174965662678 2307 20-21 -0.6731568692477907 2307 22-23 -0.6528816575245315 2307 24-25 -0.6368154716542165 2307 26-27 -0.46746108653349694 2307 28-29 -0.3221614205707297 2307 30-31 -0.3937286162406721 2307 32-33 -0.39962046744476964 2307 34-35 -0.35696481102633726 2307 36-37 -0.37482085164599965 2307 38-39 -0.4336184848700171 2307 40-41 -0.42049035398083134 2307 42-43 -0.40933460169622293 2307 44-45 -0.387144553265216 2307 46-47 -0.416284753514752 2307 48-49 -0.22677418699056062 2307 50-51 0.01278412214353608 2307 52-53 0.24153781070217306 2307 54-55 0.616065898896391 2307 56-57 0.6550996198341501 2307 58-59 0.7433688731015877 2307 60-61 0.5262396444924313 2307 62-63 0.6345093364950749 2307 64-65 0.6142941501936079 2307 66-67 0.5949965823281573 2307 68-69 0.6512457132652933 2307 70-71 0.59603098777988 2307 72-73 0.5860352274659064 2307 74-75 0.6053058277551564 2307 76-77 0.7538999841124543 2307 78-79 0.7971493194557588 2307 80-81 0.6981376661608536 2307 82-83 0.7947046192730731 2307 84-85 0.8050766612826301 2307 86-87 0.7456981696993807 2307 88-89 0.638318097511462 2307 90-91 0.5322263589402993 2307 92-93 0.43986624583577694 2307 94-95 0.4610406866319181 2307 96-97 0.5021637306939937 2307 98-99 0.5522583045753926 2307 100-101 0.5714072938059882 2308 1 -0.8179157775072099 2308 2 -0.5114006312164712 2308 3 -0.6554047287135631 2308 4 -0.5983902701102153 2308 5 -0.46442242343966456 2308 6 -0.664312543764062 2308 7 -0.5541162341000145 2308 8 -0.497680111911869 2308 9 -0.546599820844655 2308 10-11 -0.7596289839301349 2308 12-13 -1.0553696403282515 2308 14-15 -1.054877194714372 2308 16-17 -0.9877745168951293 2308 18-19 -0.9266597050242211 2308 20-21 -1.1084831453535244 2308 22-23 -1.236354358400547 2308 24-25 -1.8395993725263935 2308 26-27 -1.0980062094923824 2308 28-29 -1.1628404682970341 2308 30-31 -1.5043184782581633 2308 32-33 -1.0145115387424042 2308 34-35 -0.8604460631351714 2308 36-37 -1.2908901489565991 2308 38-39 -1.0571078199012973 2308 40-41 -1.0542037178335306 2308 42-43 -0.7625928224275711 2308 44-45 -0.9212296441711132 2308 46-47 -1.1680716145081362 2308 48-49 -0.7218616113955605 2308 50-51 -0.2778725766134258 2308 52-53 -0.1276485660240354 2308 54-55 0.23177949022228006 2308 56-57 0.5496672254592632 2308 58-59 0.3090890064946521 2308 60-61 0.38900159560128955 2308 62-63 0.348797838002465 2308 64-65 0.24163360312487825 2308 66-67 0.3458226789339598 2308 68-69 0.37933008805612545 2308 70-71 0.28520466187334037 2308 72-73 0.5361765087036332 2308 74-75 0.647450311379032 2308 76-77 0.42200972247773905 2308 78-79 0.669653589361058 2308 80-81 0.5070655361437382 2308 82-83 0.26969445493954325 2308 84-85 0.2748975071584958 2308 86-87 0.5222375029716453 2308 88-89 0.5670981444749934 2308 90-91 0.565661041011257 2308 92-93 0.5490374915687255 2308 94-95 0.2701004402344225 2308 96-97 0.2763347913872458 2308 98-99 0.34926135104845457 2308 100-101 0.4344223966435141 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 228.0 11 1603.0 12 4545.0 13 8577.0 14 13214.0 15 20092.0 16 30392.0 17 49668.0 18 98425.0 19 411201.0 20 454763.0 21 263099.0 22 211170.0 23 178753.0 24 149492.0 25 128157.0 26 112746.0 27 108201.0 28 103307.0 29 98022.0 30 98695.0 31 102357.0 32 110130.0 33 123400.0 34 148966.0 35 235591.0 36 369712.0 37 471852.0 38 433057.0 39 80039.0 40 94.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 11.677204741754917 65.76624849939327 11.515242050978953 11.041304707872868 2 11.705136023341604 13.995935827904587 64.46009874461807 9.83882940413574 3 12.0086262359841 64.40575780902958 12.724499047061563 10.861116907924758 4 12.260723343495943 12.21719094595402 63.63009974135998 11.89198596919006 5 45.14212533491331 12.12647573460124 11.730716818931313 31.00068211155414 6 45.73679069900346 11.706664807898955 29.953320108374236 12.603224384723354 7 12.957501469840773 44.4763643542615 11.709998467382524 30.856135708515204 8 30.92430255081233 11.695689708170583 44.17834818471418 13.20165955630291 9 12.376124244190125 29.752867596570056 12.044013829924486 45.82699432931533 10-11 47.732808491220354 11.485528454298992 11.770870223666904 29.010792830813752 12-13 37.880102122545324 11.533574280427436 11.960304341463711 38.626019255563534 14-15 37.63729698230217 11.880989222322185 12.47666438361502 38.00504941176063 16-17 37.27214220958414 11.841266721332909 12.770123830296818 38.116467238786136 18-19 36.73191619613001 12.021121979899332 13.704446841985407 37.542514981985256 20-21 20.18359804898661 20.804221538557453 31.24394421272384 27.768236199732094 22-23 26.98278811515027 27.947857690147544 24.469080767522712 20.600273427179474 24-25 10.370675115144277 11.335394365293183 41.84016102935136 36.45376949021118 26-27 10.289556293941802 11.219130676290149 67.90185632380413 10.589456705963924 28-29 10.335648371135708 11.27736141540645 67.88069592707619 10.50629428638164 30-31 10.23942169233873 11.20071703984892 68.02387376427305 10.535987503539307 32-33 10.145873578973527 11.233696456882795 68.14333350362416 10.477096460519514 34-35 10.112082394208265 11.245883796423373 68.17503573942733 10.466998069941042 36-37 10.079341095708932 11.200533039163139 68.32054781117114 10.399578053956795 38-39 9.968756683554322 11.226910078648388 68.45977138888914 10.34456184890816 40-41 10.014875914266936 11.290217138126932 68.41686675839281 10.278040189213316 42-43 9.962587248795769 11.250364754300637 68.47675356982977 10.310294427073817 44-45 9.945615891424875 11.42323881037712 68.33537610173116 10.295769196466841 46-47 10.074827667122412 11.73812892516757 67.75807936187697 10.428964045833055 48-49 10.378966769537703 13.011302825316667 65.78100591941423 10.828724485731406 50-51 10.767642202990821 14.735533114270288 63.241832872706304 11.25499181003259 52-53 11.38414261568528 16.028980785373438 60.724173259071925 11.862703339869363 54-55 12.743383733703157 17.77771259016277 56.19689223938798 13.2820114367461 56-57 13.775631582764102 19.065557322203812 52.563185335352294 14.595625759679795 58-59 14.301366713799704 19.787000806139478 50.34251186479716 15.569120615263657 60-61 14.658284749936573 20.051626263002355 49.06701911096064 16.223069876100432 62-63 15.06345426002717 20.01313764896479 48.551276012285186 16.372132078722853 64-65 15.345592252748538 19.820532225230693 48.312280768594675 16.52159475342609 66-67 15.544967738813906 19.63072797199459 48.07824342931824 16.746060859873264 68-69 15.621127867921277 20.095234425532542 47.735135558717005 16.54850214782918 70-71 16.043063087557485 20.509755499888733 47.07569874801604 16.371482664537744 72-73 16.563664109380458 20.981328141480585 46.06600367936629 16.389004069772664 74-75 17.024196966633486 21.608883102691205 44.73264187597496 16.63427805470035 76-77 17.393712104598073 22.36668428182023 43.38142537261895 16.858178240962747 78-79 17.66033646488039 23.17979097908362 42.119178030593304 17.040694525442685 80-81 17.76229242572396 23.959879995436726 40.96405859433431 17.313768984505003 82-83 17.936938852026216 24.47173403112166 39.97890053312575 17.612426583726375 84-85 18.12162142270196 24.74967247877931 39.19621573366052 17.932490364858207 86-87 18.349197800304275 25.08888315480216 38.56145666199377 18.000462382899798 88-89 18.61245948737842 25.456895349934666 37.846852116267655 18.08379304641926 90-91 18.837275854694006 25.59037161211443 37.32369487231219 18.248657660879374 92-93 19.055251725926432 25.620655960280097 36.82661160788891 18.49748070590456 94-95 19.360562981486503 25.605308138371978 36.122246159148034 18.911882720993482 96-97 19.601744586266882 25.7420531186168 35.60121033486393 19.054991960252387 98-99 19.79832226009991 25.90732902872749 35.15389384415965 19.140454867012963 100-101 19.947468938248935 26.23823680985315 34.432335401951406 19.381958849946514 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8568.0 1 4916.5 2 1088.0 3 875.5 4 834.5 5 851.0 6 833.5 7 850.0 8 928.0 9 1000.5 10 1598.0 11 2377.0 12 2850.0 13 5487.0 14 13409.5 15 25860.5 16 239882.5 17 291593.5 18 124279.5 19 108903.0 20 104153.5 21 102032.5 22 99756.0 23 97102.0 24 94694.5 25 92603.0 26 90774.0 27 88589.0 28 86302.5 29 84096.0 30 82164.5 31 79818.5 32 78385.0 33 78410.0 34 78054.0 35 77960.0 36 80122.5 37 82490.5 38 83313.0 39 83835.5 40 83932.5 41 83893.0 42 84005.0 43 85101.5 44 87207.0 45 90749.0 46 95399.5 47 99436.0 48 102164.5 49 103362.0 50 101134.0 51 98187.5 52 97980.5 53 97927.5 54 96186.5 55 92402.0 56 86369.0 57 79479.5 58 71287.5 59 62437.0 60 53467.5 61 44622.0 62 37273.5 63 29659.0 64 21958.5 65 15401.5 66 10851.5 67 8347.5 68 6382.5 69 4558.5 70 3231.5 71 2177.0 72 1512.5 73 1173.0 74 903.0 75 710.0 76 556.5 77 355.5 78 189.5 79 101.5 80 50.5 81 22.5 82 10.0 83 10.5 84 6.5 85 2.5 86 3.0 87 2.5 88 0.5 89 0.5 90 1.0 91 1.0 92 1.5 93 1.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012620282330652263 2 0.0033986009020795975 3 0.003290365204561139 4 0.0 5 0.0014936526257547276 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.901205918847472E-4 24-25 3.788249413146048E-4 26-27 3.138835228035297E-4 28-29 8.875327196513599E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0037233079946349733 50-51 0.0104339212407794 52-53 0.006072022630785523 54-55 0.008702150080484064 56-57 6.494141851107511E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.006440024002348281 68-69 0.0 70-71 0.0 72-73 5.411784875922926E-5 74-75 0.0027275395774651544 76-77 0.009070151452046824 78-79 0.0022945967873913204 80-81 0.001233886951710427 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.028747401260902583 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4619548.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.642836347784062 #Duplication Level Percentage of deduplicated Percentage of total 1 82.1321142427143 23.524967071515697 2 8.689008905006716 4.977557201810915 3 2.7790708787403844 2.388014171359598 4 1.5988494518304621 1.8318233277409672 5 1.037571842380654 1.4859500240188934 6 0.8084815285643745 1.389432246772541 7 0.5841281205834751 1.1711760314807802 8 0.47753712976408613 1.094241428625859 9 0.3493031527375313 0.9004529735663517 >10 1.48958947499434 6.517163560868637 >50 0.020946382017557906 0.413432029170701 >100 0.022721814148945255 1.4471485399910105 >500 0.004511440781865995 0.9349746383857052 >1k 0.0040602967036785335 2.4117491288399906 >5k 9.77478836070758E-4 2.0846921445605546 >10k+ 0.0011278601954662593 47.427225481291806 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 1274655 27.59263460407815 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 720501 15.596785659549376 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTA 27656 0.5986732901140978 No Hit GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24644 0.5334721059289784 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAA 23991 0.5193365238330676 No Hit ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16813 0.3639533564755686 No Hit CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15445 0.33434006963451834 No Hit CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14164 0.30661008393028927 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA 12851 0.27818738976194207 No Hit ATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12816 0.27742973987931285 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA 12694 0.27478878885986247 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTAAA 12537 0.2713901879577829 No Hit GGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12452 0.2695501810999691 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10560 0.2285937931589844 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10375 0.22458907235080144 No Hit ATATCTCGAGGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 9113 0.1972703822971425 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTC 8508 0.184173862897409 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC 8189 0.17726842539573134 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTAAA 8114 0.17564488993295446 No Hit TGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7721 0.16713756410800365 No Hit ATATGGATCTGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 7702 0.1667262684574335 No Hit TATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT 7556 0.1635657860898945 No Hit TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7520 0.1627864890677616 No Hit ATATGGATTCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 7320 0.15845706116702327 No Hit ATATGGACCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 7010 0.15174644792087882 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTG 6897 0.14930032115696168 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC 6023 0.13038072123073513 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG 5124 0.11091994281691628 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.001969889694835945 2.1647139503691705E-5 0.0 0.0 0.0 2 0.001969889694835945 2.1647139503691705E-5 0.0 0.0 0.0 3 0.001969889694835945 2.1647139503691705E-5 0.0 0.0 0.0 4 0.001969889694835945 2.1647139503691705E-5 0.0 0.0 0.0 5 0.001969889694835945 2.1647139503691705E-5 0.0 0.0 2.1647139503691705E-5 6 0.001969889694835945 2.1647139503691705E-5 0.0 4.329427900738341E-5 2.1647139503691705E-5 7 0.0020131839738433284 2.1647139503691705E-5 0.0 6.494141851107511E-5 2.1647139503691705E-5 8 0.00203483111334702 2.1647139503691705E-5 0.0 6.494141851107511E-5 2.1647139503691705E-5 9 0.00203483111334702 2.1647139503691705E-5 0.0 8.658855801476682E-5 2.1647139503691705E-5 10-11 0.00203483111334702 2.1647139503691705E-5 0.0 1.1905926727030436E-4 2.1647139503691705E-5 12-13 0.002067301822602558 2.1647139503691705E-5 0.0 1.2988283702215021E-4 2.1647139503691705E-5 14-15 0.0021214196713617867 2.1647139503691705E-5 0.0 1.5152997652584192E-4 2.1647139503691705E-5 16-17 0.002186361089872862 2.1647139503691705E-5 0.0 1.9482425553322532E-4 2.1647139503691705E-5 18-19 0.0022729496478876288 2.1647139503691705E-5 0.0 1.9482425553322532E-4 2.1647139503691705E-5 20-21 0.002402832484909779 2.1647139503691705E-5 0.0 2.3811853454060874E-4 2.1647139503691705E-5 22-23 0.002565186031187467 2.1647139503691705E-5 0.0 2.3811853454060874E-4 7.576498826292096E-5 24-25 0.002781657426224384 2.1647139503691705E-5 0.0 2.3811853454060874E-4 1.0823569751845852E-4 26-27 0.003041423100268684 4.329427900738341E-5 0.0 2.3811853454060874E-4 1.0823569751845852E-4 28-29 0.003192953076794526 4.329427900738341E-5 0.0 2.3811853454060874E-4 1.0823569751845852E-4 30-31 0.003290365204561139 4.329427900738341E-5 0.0 2.489421042924546E-4 1.0823569751845852E-4 32-33 0.003485189460094364 4.329427900738341E-5 0.0 2.5976567404430043E-4 1.0823569751845852E-4 34-35 0.0036042487273646685 4.329427900738341E-5 0.0 2.8141281354799214E-4 1.1905926727030436E-4 36-37 0.003701660855131281 4.329427900738341E-5 0.0 2.8141281354799214E-4 1.4070640677399607E-4 38-39 0.003799072982897894 4.329427900738341E-5 0.0 2.92236383299838E-4 1.6235354627768778E-4 40-41 0.003918132250168198 4.329427900738341E-5 0.0 3.0305995305168385E-4 2.0564782528507118E-4 42-43 0.0042428393427235735 4.329427900738341E-5 0.0 3.0305995305168385E-4 2.272949647887629E-4 44-45 0.004556722865527104 4.329427900738341E-5 0.0 3.0305995305168385E-4 2.489421042924546E-4 46-47 0.0049788420858490915 4.329427900738341E-5 0.0 3.0305995305168385E-4 2.8141281354799214E-4 48-49 0.005422608445674772 4.329427900738341E-5 0.0 3.0305995305168385E-4 2.8141281354799214E-4 50-51 0.006050375491281831 4.329427900738341E-5 0.0 3.0305995305168385E-4 2.92236383299838E-4 52-53 0.007500733838029175 6.494141851107511E-5 0.0 3.2470709255537556E-4 3.0305995305168385E-4 54-55 0.009145916440309744 6.494141851107511E-5 0.0 3.2470709255537556E-4 3.4635423205906727E-4 56-57 0.010953452588868002 6.494141851107511E-5 0.0 3.2470709255537556E-4 3.4635423205906727E-4 58-59 0.01372428644534054 6.494141851107511E-5 0.0 3.2470709255537556E-4 3.4635423205906727E-4 60-61 0.016982180940646144 6.494141851107511E-5 0.0 3.2470709255537556E-4 3.4635423205906727E-4 62-63 0.02144149167840663 6.494141851107511E-5 0.0 3.2470709255537556E-4 3.4635423205906727E-4 64-65 0.02585750813715974 6.494141851107511E-5 0.0 3.2470709255537556E-4 3.8964851106645064E-4 66-67 0.031951177907448954 6.494141851107511E-5 0.0 3.2470709255537556E-4 4.1129565057014235E-4 68-69 0.03928955819920044 6.494141851107511E-5 0.0 3.2470709255537556E-4 4.3294279007383406E-4 70-71 0.04659546778169639 6.494141851107511E-5 0.0 3.2470709255537556E-4 4.545899295775258E-4 72-73 0.05755974394031624 6.494141851107511E-5 0.0 3.2470709255537556E-4 4.762370690812175E-4 74-75 0.06923837570255792 6.494141851107511E-5 0.0 3.2470709255537556E-4 5.08707778336755E-4 76-77 0.08370948846077582 6.494141851107511E-5 0.0 3.2470709255537556E-4 5.303549178404467E-4 78-79 0.10035613873911474 6.494141851107511E-5 0.0 3.4635423205906727E-4 5.84472766599676E-4 80-81 0.12129974620893645 6.494141851107511E-5 0.0 3.4635423205906727E-4 6.277670456070594E-4 82-83 0.14677842940478159 6.494141851107511E-5 0.0 3.4635423205906727E-4 6.494141851107511E-4 84-85 0.17763642676729413 6.494141851107511E-5 0.0 3.4635423205906727E-4 6.60237754862597E-4 86-87 0.21260738063550805 6.494141851107511E-5 0.0 3.571778018109131E-4 6.927084641181345E-4 88-89 0.256356249572469 6.494141851107511E-5 0.0 3.6800137156275893E-4 7.143556036218262E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATATGGA 153035 0.0 92.49397 1 TATGGAT 153420 0.0 91.64594 2 ATGGATC 153930 0.0 90.68405 3 ATATCTC 89860 0.0 90.125885 1 TGGATCC 153895 0.0 89.844696 4 TATCTCG 89960 0.0 89.8245 2 GGATCCG 154115 0.0 89.51611 5 ATCTCGA 91420 0.0 88.274704 3 TCTCGAG 91795 0.0 87.88632 4 GATCCGG 158040 0.0 87.55948 6 CTCGAGG 92710 0.0 87.10091 5 TCGAGGG 92815 0.0 87.05772 6 CGAGGGC 93280 0.0 86.664474 7 ATCCGGC 160090 0.0 86.41155 7 AGGGCGC 94280 0.0 86.178505 9 GAGGGCG 94365 0.0 85.959946 8 TCCGGCG 161945 0.0 85.64171 8 CCGGCGC 165605 0.0 84.21073 9 ATCGGCG 450 0.0 66.49639 7 TATCGAT 320 0.0 65.31178 2 >>END_MODULE