FastQCFastQC Report
Tue 31 May 2016
SRR1051264_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051264_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4619548
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA623721.3501753851242588No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT181770.39348005475860415No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117080.25344470930922247No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86270.18674987249834832No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT74060.16031871516434076No Hit
CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62090.13440708917842178No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT60140.13018589697520191No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49070.10622251354461519No Hit
ATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46660.10100555292422547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG14600.059.8612062
ATATGGA39950.049.2284051
ATATCTC20800.042.936511
TATGGAT47200.041.5612452
GGATCCG59150.039.8284455
ATGGATC59500.037.519473
TCTCGAG28100.035.157564
ATCTCGA27000.034.6559833
GATCCGG68900.033.778736
ATCCGGC70950.033.003577
TCGAGGG32100.031.368756
CTCGAGG32600.030.7419385
TGGATCC77650.030.0943764
TCCGGCG79050.029.7419688
CGAGGGC39550.025.7000337
CCGGCGC95750.024.5049939
AGGGCGC42350.023.6643969
GGGCGCG38400.021.769478
GAGGGCG63300.016.132488
CGCGCCG129250.014.8277312-13
Produced by FastQC (version 0.11.5)