FastQCFastQC Report
Tue 31 May 2016
SRR1051263_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051263_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4735940
Sequences flagged as poor quality0
Sequence length101
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT122104825.782590151057654No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT89580718.915083383657734No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTA284290.6002820981684734No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAA268790.567553642993788No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232060.48999776179596866No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA227450.4802636857730461No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTAAA160710.3393412923305616No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151000.318838498798549No Hit
CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148780.31415093941223915No Hit
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145460.30714071546514526No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT138840.2931624978356989No Hit
GGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111560.23556041672825245No Hit
ATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT109900.2320553047547055No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA102820.21710579103620403No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA97990.2069071821011246No Hit
ATATCTCGAGGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT83570.1764591612224817No Hit
ATATGGATCTGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT79270.16737965430305282No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTC79130.1670840424498621No Hit
ATATGGATTCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT76030.16053835141492503No Hit
ATATGGACCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT75790.16003158823802666No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73570.1553440288517169No Hit
TGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70670.14922064046419506No Hit
TATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT68700.1450609593871544No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTG63490.13405997542198592No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTAAA62800.13260303128840314No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT62600.13218072864098787No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC59570.1257828435326461No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTAAAA55350.11687225767218336No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54210.11446513258191615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGGA1492250.092.361991
TATGGAT1495350.091.449012
ATGGATC1497500.090.6991963
ATATCTC1060400.090.573721
TATCTCG1063300.090.1502762
TGGATCC1495800.089.8655244
GGATCCG1495150.089.647265
ATCTCGA1081050.088.542663
TCTCGAG1087050.088.001524
GATCCGG1531950.087.749286
CTCGAGG1097750.087.2995155
TCGAGGG1100250.087.112276
CGAGGGC1104500.086.738367
ATCCGGC1550400.086.6897357
AGGGCGC1119950.086.0888759
GAGGGCG1119850.085.960848
TCCGGCG1571100.085.846858
CCGGCGC1604250.084.591059
TATCGAT4000.068.872872
GTATCCG4200.064.462295
Produced by FastQC (version 0.11.5)