##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1051263_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4735940
Sequences flagged as poor quality	0
Sequence length	101
%GC	35
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07432209867524	33.0	31.0	34.0	30.0	34.0
2	32.40199981418684	34.0	31.0	34.0	31.0	34.0
3	32.38705790191599	34.0	31.0	34.0	31.0	34.0
4	35.85271371681229	37.0	35.0	37.0	35.0	37.0
5	35.85244618808515	37.0	35.0	37.0	35.0	37.0
6	35.775658475402984	37.0	36.0	37.0	33.0	37.0
7	35.718540775432125	37.0	36.0	37.0	33.0	37.0
8	35.76161416740921	37.0	36.0	37.0	33.0	37.0
9	37.39218296684502	39.0	37.0	39.0	34.0	39.0
10-11	37.36621262093692	39.0	37.0	39.0	34.0	39.0
12-13	37.41279397543043	39.0	38.0	39.0	35.0	39.0
14-15	39.00587444097687	41.0	39.0	41.0	36.0	41.0
16-17	38.975663543034756	41.0	39.0	41.0	35.5	41.0
18-19	38.98705811306731	41.0	39.0	41.0	35.0	41.0
20-21	38.200057538735706	40.0	38.0	41.0	33.0	41.0
22-23	38.09230195061593	40.0	38.0	41.0	33.0	41.0
24-25	38.38676260678979	40.0	38.0	41.0	33.5	41.0
26-27	38.70698921439038	40.5	38.0	41.0	34.5	41.0
28-29	38.74195904086623	41.0	38.0	41.0	35.0	41.0
30-31	38.252825943740845	40.0	38.0	40.5	34.5	41.0
32-33	36.07376750972352	36.0	35.0	39.0	33.0	41.0
34-35	35.802723535348846	35.0	35.0	39.0	33.0	41.0
36-37	35.6585484613403	35.0	35.0	39.0	33.0	41.0
38-39	35.575396014307614	35.0	35.0	39.0	33.0	41.0
40-41	35.51165523634168	35.0	35.0	39.0	33.0	41.0
42-43	35.44141817675055	35.0	35.0	38.5	33.0	41.0
44-45	35.31313361233462	35.0	35.0	38.0	33.0	41.0
46-47	34.8491547612512	35.0	35.0	38.0	30.5	41.0
48-49	33.72649347331259	35.0	34.0	38.0	23.5	41.0
50-51	32.61825646017475	35.0	32.0	37.0	20.0	40.0
52-53	30.547470935020293	35.0	30.0	37.5	7.5	40.0
54-55	28.809087002791422	35.0	24.5	38.0	2.0	41.0
56-57	27.349297288394702	35.0	17.5	37.0	2.0	41.0
58-59	26.411650168709908	35.0	8.5	37.0	2.0	40.5
60-61	25.716315029328918	35.0	3.5	36.0	2.0	40.0
62-63	25.096807603136867	35.0	2.0	36.0	2.0	40.0
64-65	24.507157818722366	34.0	2.0	35.0	2.0	40.0
66-67	23.81171520331761	33.5	2.0	35.0	2.0	39.0
68-69	23.096082087188606	33.0	2.0	35.0	2.0	39.0
70-71	22.5550103041846	33.0	2.0	35.0	2.0	39.0
72-73	21.916021528988963	33.0	2.0	35.0	2.0	37.5
74-75	21.252183304687136	31.5	2.0	35.0	2.0	37.0
76-77	20.628861746559288	31.0	2.0	35.0	2.0	36.5
78-79	20.134721195792174	30.0	2.0	35.0	2.0	36.0
80-81	19.704715431361038	29.0	2.0	35.0	2.0	36.0
82-83	19.319456327571718	28.0	2.0	35.0	2.0	35.0
84-85	18.942127539622547	26.5	2.0	35.0	2.0	35.0
86-87	18.622526890121073	25.0	2.0	35.0	2.0	35.0
88-89	18.310556721580088	23.5	2.0	35.0	2.0	35.0
90-91	18.01920241810496	19.0	2.0	35.0	2.0	35.0
92-93	17.734601472991635	7.0	2.0	35.0	2.0	35.0
94-95	17.441014877722267	2.0	2.0	35.0	2.0	35.0
96-97	17.140575049514986	2.0	2.0	35.0	2.0	35.0
98-99	16.767923052234615	2.0	2.0	35.0	2.0	35.0
100-101	16.34395991080968	2.0	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.561649256000166
1101	2	0.4772679257515051
1101	3	0.5046461236117707
1101	4	0.3904334268797953
1101	5	0.40883423764790194
1101	6	0.441234416682569
1101	7	0.5702228717179665
1101	8	0.4708795836245301
1101	9	0.5270719819935579
1101	10-11	0.5181721198145013
1101	12-13	0.478870420455344
1101	14-15	0.663016884976912
1101	16-17	0.6715948080042153
1101	18-19	0.6749165226599203
1101	20-21	0.6205622408891855
1101	22-23	0.6560029143786039
1101	24-25	0.6443022126044937
1101	26-27	0.5416921623924154
1101	28-29	0.5359747622143729
1101	30-31	0.4683611677273589
1101	32-33	0.5000493113575359
1101	34-35	0.49623657383619246
1101	36-37	0.5417086477509798
1101	38-39	0.5872549164439818
1101	40-41	0.5427782465704141
1101	42-43	0.5055583928534375
1101	44-45	0.3761052758032264
1101	46-47	0.3531099676321148
1101	48-49	0.25677426267504444
1101	50-51	0.07958414924941337
1101	52-53	-0.07112655207849627
1101	54-55	-0.26007515641697765
1101	56-57	-0.194107466708207
1101	58-59	-0.2512823013043608
1101	60-61	-0.35193005856270076
1101	62-63	-0.34170350531501725
1101	64-65	-0.3861836801028673
1101	66-67	-0.24000034267757897
1101	68-69	-0.25999506074109746
1101	70-71	-0.4001332986447501
1101	72-73	-0.36007739662986893
1101	74-75	-0.3918722231373124
1101	76-77	-0.31793057502772015
1101	78-79	-0.4343201368023344
1101	80-81	-0.3999555094558893
1101	82-83	-0.3896213603287073
1101	84-85	-0.3363559299672758
1101	86-87	-0.32907131953258784
1101	88-89	-0.3745517693345768
1101	90-91	-0.32332894944533663
1101	92-93	-0.25587438806095264
1101	94-95	-0.17936481710697905
1101	96-97	-0.17667783407774706
1101	98-99	-0.07485081550661121
1101	100-101	-0.03835393990463487
1102	1	0.5024627871982474
1102	2	0.3560093938029567
1102	3	0.3580554676473895
1102	4	0.329238710210241
1102	5	0.33620691841764483
1102	6	0.36287352887579516
1102	7	0.4378511854153828
1102	8	0.3344772779066574
1102	9	0.44608809184511244
1102	10-11	0.4704822555327368
1102	12-13	0.40241370551952116
1102	14-15	0.5060275663262246
1102	16-17	0.4991338370639582
1102	18-19	0.5475414109130767
1102	20-21	0.5462621757421005
1102	22-23	0.6561617605932355
1102	24-25	0.718625838735143
1102	26-27	0.5626777626164028
1102	28-29	0.5707380768360295
1102	30-31	0.5071756546880408
1102	32-33	0.550972859309617
1102	34-35	0.4841109039651528
1102	36-37	0.5188139688074074
1102	38-39	0.5611097964839757
1102	40-41	0.4674443641951598
1102	42-43	0.4508213028329564
1102	44-45	0.4522727070463972
1102	46-47	0.4560440769467675
1102	48-49	0.5449160093218879
1102	50-51	0.36991946219595206
1102	52-53	0.2151092659199918
1102	54-55	-0.10740466287853678
1102	56-57	-0.07964735651200172
1102	58-59	-0.07033056512065983
1102	60-61	-0.02527461802691633
1102	62-63	-0.08932037315117114
1102	64-65	-0.040625953149852734
1102	66-67	0.07508286580836199
1102	68-69	-0.0778823877540944
1102	70-71	-0.10217523290932107
1102	72-73	-0.14981135581522054
1102	74-75	-0.12457950047059185
1102	76-77	-0.10724771136613498
1102	78-79	-0.0730110790023275
1102	80-81	-0.09133638913806408
1102	82-83	-0.048249910204003044
1102	84-85	-0.018084249079198145
1102	86-87	-0.0526279756163035
1102	88-89	-0.017519417354289857
1102	90-91	0.08988946722403668
1102	92-93	0.08189132141525945
1102	94-95	0.17149910800105772
1102	96-97	0.19713877517887113
1102	98-99	0.38090984239144277
1102	100-101	0.3534523775528804
1103	1	0.3883630920087313
1103	2	0.18450723030967708
1103	3	0.22618164805851393
1103	4	0.2126882047553167
1103	5	0.2365799045812551
1103	6	0.21401557936370352
1103	7	0.32413659920322857
1103	8	0.27524720212186793
1103	9	0.34924707122925724
1103	10-11	0.2999817025656881
1103	12-13	0.3455840898490692
1103	14-15	0.3207270863190246
1103	16-17	0.387829259923798
1103	18-19	0.3638478459798691
1103	20-21	0.42042446071363315
1103	22-23	0.5288790022881145
1103	24-25	0.5715752839358572
1103	26-27	0.47575189248339456
1103	28-29	0.4732400564270449
1103	30-31	0.5070517363619729
1103	32-33	0.3743483101706815
1103	34-35	0.33912578385865544
1103	36-37	0.3713608672768771
1103	38-39	0.31848746900455893
1103	40-41	0.3845737231004378
1103	42-43	0.34921148671776336
1103	44-45	0.3633121455017658
1103	46-47	0.3765455145253753
1103	48-49	0.34359834041536885
1103	50-51	0.3584817982762232
1103	52-53	0.1301347034706808
1103	54-55	-0.1299757934033856
1103	56-57	-0.0990432041404965
1103	58-59	-0.2172094204517201
1103	60-61	-0.19514543662561223
1103	62-63	-0.26438180154726965
1103	64-65	-0.20182139830674828
1103	66-67	-0.1308417268001456
1103	68-69	-0.04928351406577036
1103	70-71	-0.05636275136498625
1103	72-73	-0.03752075185382964
1103	74-75	-0.04075522387926256
1103	76-77	-0.009613594074686205
1103	78-79	-0.02461242950607101
1103	80-81	-0.016597582212945383
1103	82-83	-0.03992048047163266
1103	84-85	0.016465830695796768
1103	86-87	0.01060627621365029
1103	88-89	0.053504699628874164
1103	90-91	0.057973784907868975
1103	92-93	0.06002418415786437
1103	94-95	0.043601484072553376
1103	96-97	0.08776208169304311
1103	98-99	0.19098820007155126
1103	100-101	0.3243029846436194
1104	1	0.2614663091952423
1104	2	0.27067856824158554
1104	3	0.21609804929551757
1104	4	0.20252873467978105
1104	5	0.19405600151987557
1104	6	0.2542593178666337
1104	7	0.2683645845369895
1104	8	0.22805426753553348
1104	9	0.319761936564646
1104	10-11	0.33089785136305494
1104	12-13	0.285875059031099
1104	14-15	0.3293927190610333
1104	16-17	0.29018927262988115
1104	18-19	0.3174251828635235
1104	20-21	0.36474523886636234
1104	22-23	0.48348457085567276
1104	24-25	0.5336825104737457
1104	26-27	0.5199019747994953
1104	28-29	0.3997067395212568
1104	30-31	0.41536322277497817
1104	32-33	0.31145357808635765
1104	34-35	0.27633593138361334
1104	36-37	0.28262287412570686
1104	38-39	0.26771971720871335
1104	40-41	0.24428049846711275
1104	42-43	0.16118479499212413
1104	44-45	0.18570731963654907
1104	46-47	0.27540275853570506
1104	48-49	0.4748438379454356
1104	50-51	0.44876809802196505
1104	52-53	0.40230811142564704
1104	54-55	0.36427642060426635
1104	56-57	0.5328013372041518
1104	58-59	0.398466610258037
1104	60-61	0.3297155731563457
1104	62-63	0.27802414568425604
1104	64-65	0.28958610808699703
1104	66-67	0.291985719303117
1104	68-69	0.23689749442273467
1104	70-71	0.12608860756802542
1104	72-73	0.19674836364677262
1104	74-75	0.28169128257285436
1104	76-77	0.2634558498088282
1104	78-79	0.3063869090148117
1104	80-81	0.17020403742144907
1104	82-83	0.06528592187983051
1104	84-85	0.015663745669375118
1104	86-87	-0.047055679580580545
1104	88-89	-0.06339491902670957
1104	90-91	-0.04651963735965481
1104	92-93	0.008134648921146237
1104	94-95	0.027297112292867354
1104	96-97	0.06998810237807263
1104	98-99	0.1806376575174582
1104	100-101	0.3136979280162713
1105	1	0.07492115805878541
1105	2	0.06532338880331423
1105	3	0.01643012692182566
1105	4	0.03635158443416486
1105	5	-0.015091393698270394
1105	6	-0.02809350438477054
1105	7	0.10725268362583762
1105	8	0.018383195942867303
1105	9	0.11379032361461583
1105	10-11	0.05420555672727545
1105	12-13	0.054533763384227996
1105	14-15	0.051730531871967855
1105	16-17	0.01800727101339561
1105	18-19	0.019466012469287364
1105	20-21	-0.020937989110315414
1105	22-23	-0.3461825061663646
1105	24-25	-0.6401714050426719
1105	26-27	-0.4208489883986388
1105	28-29	-0.7435795507680467
1105	30-31	-0.43572738698040325
1105	32-33	-0.2031059693694388
1105	34-35	-0.013053500039902133
1105	36-37	0.09287796622241018
1105	38-39	0.08619654017697087
1105	40-41	0.024023385930661334
1105	42-43	0.019499891877075015
1105	44-45	-0.021064868984453256
1105	46-47	-0.2648624897868288
1105	48-49	-0.44256192898222224
1105	50-51	-0.6106587241862655
1105	52-53	-0.27061648005543404
1105	54-55	-0.22884665335589816
1105	56-57	0.1365294312496168
1105	58-59	0.3529330556182302
1105	60-61	0.4666472116500202
1105	62-63	0.5142333258172869
1105	64-65	0.5105678797283808
1105	66-67	0.44502431898571615
1105	68-69	0.4332388304826864
1105	70-71	0.4606933968806892
1105	72-73	0.22036132607133396
1105	74-75	-0.015371269440226598
1105	76-77	-0.04299228698831925
1105	78-79	0.0706621874143245
1105	80-81	-0.012047609551039784
1105	82-83	0.15125838854284268
1105	84-85	0.34750274719309004
1105	86-87	0.3849197541530991
1105	88-89	0.322523080019284
1105	90-91	0.41101959586501735
1105	92-93	0.3615409444133384
1105	94-95	0.35411649267518897
1105	96-97	0.4012326671131987
1105	98-99	0.4227578138245427
1105	100-101	0.12774846786753002
1106	1	-0.07007789176587664
1106	2	-0.180852591786973
1106	3	-0.19344008424114634
1106	4	-0.23997324451195112
1106	5	-0.1963476691781736
1106	6	-0.25082312718261335
1106	7	-0.01248481227371201
1106	8	-0.10193895611457293
1106	9	-0.15257816144384861
1106	10-11	-0.15917183034979843
1106	12-13	-0.19929181868035784
1106	14-15	-0.21338804237959152
1106	16-17	-0.23035114989828998
1106	18-19	-0.1604884903268058
1106	20-21	-0.2078149718047797
1106	22-23	-0.10869268473194893
1106	24-25	-0.018090495075860247
1106	26-27	0.03726549884072483
1106	28-29	0.005366326903761376
1106	30-31	-0.010004827544925377
1106	32-33	-0.04754489468825085
1106	34-35	-0.12584898285520296
1106	36-37	-0.15762656835849498
1106	38-39	-0.13886496957168504
1106	40-41	-0.12351251231055471
1106	42-43	-0.16760069252337217
1106	44-45	-0.06555103518647343
1106	46-47	0.040746126132006566
1106	48-49	0.18144603885290422
1106	50-51	0.16251914108755727
1106	52-53	0.2926559128497388
1106	54-55	0.2995511488938227
1106	56-57	0.6496934286717639
1106	58-59	0.5779916531464444
1106	60-61	0.48159310239130093
1106	62-63	0.4621673601075358
1106	64-65	0.3839742863211093
1106	66-67	0.4875459236025357
1106	68-69	0.504467084922517
1106	70-71	0.6209812894551057
1106	72-73	0.6015969379773516
1106	74-75	0.6400722509768535
1106	76-77	0.6921090570653377
1106	78-79	0.615334563223648
1106	80-81	0.578435272315744
1106	82-83	0.535049225280801
1106	84-85	0.47846394066851516
1106	86-87	0.44464240401961774
1106	88-89	0.352389189314696
1106	90-91	0.3035799908522172
1106	92-93	0.32105987279089376
1106	94-95	0.3507233398310632
1106	96-97	0.3872457086654464
1106	98-99	0.37080073674734493
1106	100-101	0.4627510379274362
1107	1	-0.3761930011983914
1107	2	-0.5008430024507824
1107	3	-0.552965715206728
1107	4	-0.5174309002185922
1107	5	-0.3458789581386199
1107	6	-0.520017576949023
1107	7	-0.22453326880754787
1107	8	-0.3021080927629214
1107	9	-0.31369005545536766
1107	10-11	-0.37698826166755595
1107	12-13	-0.4105147802470839
1107	14-15	-0.4569818278292388
1107	16-17	-0.391215110785744
1107	18-19	-0.5058423295183516
1107	20-21	-0.5817110916523376
1107	22-23	-0.8169288278282494
1107	24-25	-0.5124855250581888
1107	26-27	-0.5461587005782604
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2308	20-21	-1.1900284124528255
2308	22-23	-1.3361055278281881
2308	24-25	-2.067412430841024
2308	26-27	-1.1855782859297577
2308	28-29	-1.3141411526128692
2308	30-31	-1.6560170975510502
2308	32-33	-1.1367468581356164
2308	34-35	-0.9594018225461625
2308	36-37	-1.323177807919926
2308	38-39	-1.1522558142966446
2308	40-41	-1.1277342562977495
2308	42-43	-0.8424294496270548
2308	44-45	-1.1122547991645106
2308	46-47	-1.1547256876986154
2308	48-49	-0.8833343711973285
2308	50-51	-0.3987338566805292
2308	52-53	-0.20135885700130274
2308	54-55	0.3574185550429476
2308	56-57	0.8041978900722135
2308	58-59	0.6412791845869883
2308	60-61	0.7482158721860301
2308	62-63	0.732500916939852
2308	64-65	0.7805761118854342
2308	66-67	0.8669632731949797
2308	68-69	0.8391088442114736
2308	70-71	0.7441220902941943
2308	72-73	0.9809166387526886
2308	74-75	1.0746693544652395
2308	76-77	0.9299650770852175
2308	78-79	1.0966347707521322
2308	80-81	0.9062176806580631
2308	82-83	0.5753577726650043
2308	84-85	0.6469663786992434
2308	86-87	0.9582793945970209
2308	88-89	1.0112773654706082
2308	90-91	0.9836736228470642
2308	92-93	0.9231518650599462
2308	94-95	0.6571804146077511
2308	96-97	0.6583545055941826
2308	98-99	0.6989267417778322
2308	100-101	0.7697568840976636
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	162.0
11	1271.0
12	3762.0
13	7414.0
14	11915.0
15	18761.0
16	29410.0
17	47982.0
18	93975.0
19	386541.0
20	450143.0
21	266586.0
22	211400.0
23	178442.0
24	149296.0
25	127678.0
26	112447.0
27	108025.0
28	104288.0
29	100238.0
30	100982.0
31	106208.0
32	115403.0
33	131081.0
34	162264.0
35	282606.0
36	437866.0
37	468374.0
38	437778.0
39	83521.0
40	120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.239742758292852	66.71189422958948	11.61004562950749	10.438317382610188
2	11.363210683696884	13.800781673145416	65.27403642810552	9.561971215052182
3	11.675697567685303	65.22177881264257	12.650511204391593	10.452012415280535
4	11.937883503591685	12.138287224922612	64.47955421732539	11.444275054160315
5	42.9225192482991	12.012146440582338	11.497309999655817	33.56802431146274
6	43.47605755140479	11.619150580454988	32.624885450406886	12.279906417733333
7	12.639138164757155	42.55769709920311	11.533486488426796	33.26967824761293
8	33.57992288753658	11.663006710389068	42.024877848959235	12.732192553115116
9	12.091960624501155	32.68322656114731	11.858300569686271	43.36651224466526
10-11	49.31993437416859	11.467417239238673	11.632685380304649	27.57996300628809
12-13	38.0336744131049	11.510513224407404	11.8064945924146	38.6493177700731
14-15	37.75150234166818	11.833785901003814	12.277583753172548	38.13712800415546
16-17	37.42288753658197	11.785432247874763	12.585431825572114	38.20624838997116
18-19	36.959779895860166	11.957721170454018	13.489666254217747	37.59283267946807
20-21	21.345825749481623	22.250725304796937	29.991501159220768	26.411947786500672
22-23	25.65731177361344	26.882137173730143	25.679218910789807	21.781332141866606
24-25	10.062316330468173	11.272725929030647	42.01003290281133	36.65492483768985
26-27	9.97618203010186	11.145754151252554	68.57544046352135	10.302623355124242
28-29	10.066960050854291	11.201447417361512	68.55474802114537	10.176844510638828
30-31	9.952712661055672	11.124106302022408	68.70134545623466	10.221835580687255
32-33	9.856649366334876	11.174835407543169	68.81500821378648	10.15350701233546
34-35	9.817618044147519	11.169070976405951	68.89846577448195	10.11484520496459
36-37	9.771988243094295	11.123557308580768	69.02134528731361	10.08310916101133
38-39	9.63626017221502	11.156180188093598	69.18972791040427	10.01783172928711
40-41	9.69039937161366	11.218955476631882	69.15591202591249	9.934733125841966
42-43	9.647556768033379	11.186670439236984	69.17671043129769	9.989062361431944
44-45	9.604239073974755	11.349320303888986	69.10480284800904	9.941637774127205
46-47	9.710395401968775	11.64859563254602	68.59751179280143	10.043497172683775
48-49	9.97150497826158	12.916873911666805	66.77014223438599	10.341478875685624
50-51	10.31907303691404	14.550460724660038	64.45713743900885	10.673328799417074
52-53	10.880329332292291	15.789536477890803	62.127166482689525	11.202967707127385
54-55	12.218439569241433	17.523542624215594	57.7252296032114	12.532788203331577
56-57	13.178116649952273	18.844110834631827	54.1906213520298	13.787151163386097
58-59	13.63670147848157	19.707914373915212	51.96686402277055	14.688520124832664
60-61	13.993621118510791	20.101500441306268	50.5600366558698	15.344841784313147
62-63	14.373915210074454	20.149653500677793	49.909479427526534	15.56695186172122
64-65	14.645645848553823	20.03593795529504	49.62238753024742	15.696028665903707
66-67	14.888363720561038	19.920994580545816	49.24596534205784	15.944676356835302
68-69	14.962066664695922	20.302315907718427	48.92165018982504	15.813967237760613
70-71	15.368775786855407	20.60769350963061	48.331461124929795	15.692069578584189
72-73	15.911265767725096	20.987470280451188	47.34653521792928	15.754728733894433
74-75	16.331859315242507	21.54861430193116	46.12133768936579	15.998188693460547
76-77	16.7541231126597	22.236574807306514	44.81830852074754	16.190993559286245
78-79	16.99767833972256	22.978978363899923	43.63500839873011	16.388334897647404
80-81	17.155504991109872	23.726134403379483	42.48733152648494	16.6310290790257
82-83	17.400157096584838	24.216216843963394	41.47276992529466	16.910856134157104
84-85	17.562616925045504	24.494144773793586	40.61788156099951	17.325356740161403
86-87	17.757118966878803	24.78702221734228	39.982981203309166	17.472877612469752
88-89	17.945772117045404	25.180777205792303	39.26299741973082	17.610453257431473
90-91	18.182821150605793	25.323663306545267	38.695623255362186	17.79789228748675
92-93	18.429150284843136	25.35024725820006	38.126528207705334	18.09407424925147
94-95	18.730452666207764	25.367456091082236	37.411242541079496	18.49084870163051
96-97	19.031755047572393	25.534381770039317	36.84638107746297	18.587482104925314
98-99	19.317727842835847	25.682198254200856	36.36643411867549	18.63363978428781
100-101	19.528824341963528	25.954527789394533	35.67570450349981	18.840943365142127
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	20246.0
1	11304.5
2	1850.0
3	1184.5
4	1061.0
5	1044.5
6	1006.0
7	1043.5
8	1109.0
9	1211.5
10	1847.0
11	2708.0
12	3273.5
13	6281.0
14	14408.5
15	27494.0
16	305457.0
17	355931.5
18	122927.0
19	106220.0
20	101665.5
21	99832.5
22	97748.0
23	95587.5
24	93661.5
25	91447.0
26	89889.0
27	88401.5
28	86739.0
29	85153.5
30	83866.5
31	82447.5
32	81419.5
33	81942.5
34	82441.5
35	82619.5
36	84902.5
37	86911.0
38	86542.5
39	85806.5
40	85161.5
41	84642.0
42	83728.0
43	83828.5
44	85762.5
45	89077.5
46	92526.0
47	95542.0
48	98014.0
49	99309.0
50	98017.5
51	95463.5
52	94752.0
53	94596.0
54	92549.0
55	89609.5
56	84070.5
57	76911.0
58	69164.5
59	60171.0
60	51657.5
61	43444.5
62	35997.0
63	28531.5
64	21229.5
65	14985.5
66	10497.5
67	7979.5
68	6087.5
69	4445.5
70	3272.0
71	2251.5
72	1632.0
73	1260.5
74	1010.0
75	810.0
76	609.5
77	370.5
78	209.0
79	124.5
80	65.0
81	34.5
82	14.5
83	6.0
84	2.5
85	1.5
86	1.5
87	0.5
88	0.5
89	0.5
90	0.5
91	1.0
92	0.5
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01323918799646955
2	0.004244141606523731
3	0.0034417665764346675
4	0.0
5	0.0014147138688412437
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	8.551628610159758E-4
24-25	4.7509047834220873E-4
26-27	4.2230264741529666E-4
28-29	0.001193004978948213
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.004233584040338348
50-51	0.01096931126661233
52-53	0.006281751880302538
54-55	0.009153409882726555
56-57	3.167269855614725E-5
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.006661824262976305
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0027871974729409576
76-77	0.009290658243136525
78-79	0.0022170888989303072
80-81	0.0014675016997681557
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.029561185319070763
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4735940.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.914135895815996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.77597379099674	23.10619781310386
2	8.531161014989195	4.762799758429917
3	2.7400992034626124	2.294625046004175
4	1.5029843625323283	1.6781803898005512
5	0.9729768523061041	1.3579904039377937
6	0.7388675601278821	1.2374909689451816
7	0.5611212325315471	1.096425003723936
8	0.4061141284524632	0.9069059976666344
9	0.3077699775929613	0.7732019681263947
>10	1.3866919305202474	6.273910874264814
>50	0.04261912241304751	0.7687430851151104
>100	0.022643739735335143	1.4321214092517076
>500	0.0047366870779673624	0.9177993423917183
>1k	0.0040600174953995686	2.3670832277097014
>5k	0.0011277826376109913	2.2304133752559787
>10k+	0.0010525971284369251	48.79611133627256
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	1221048	25.782590151057654	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	895807	18.915083383657734	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTA	28429	0.6002820981684734	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAA	26879	0.567553642993788	No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23206	0.48999776179596866	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22745	0.4802636857730461	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTAAA	16071	0.3393412923305616	No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15100	0.318838498798549	No Hit
CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14878	0.31415093941223915	No Hit
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14546	0.30714071546514526	No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13884	0.2931624978356989	No Hit
GGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11156	0.23556041672825245	No Hit
ATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10990	0.2320553047547055	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA	10282	0.21710579103620403	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA	9799	0.2069071821011246	No Hit
ATATCTCGAGGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	8357	0.1764591612224817	No Hit
ATATGGATCTGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	7927	0.16737965430305282	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTC	7913	0.1670840424498621	No Hit
ATATGGATTCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	7603	0.16053835141492503	No Hit
ATATGGACCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	7579	0.16003158823802666	No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7357	0.1553440288517169	No Hit
TGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7067	0.14922064046419506	No Hit
TATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6870	0.1450609593871544	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTG	6349	0.13405997542198592	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTAAA	6280	0.13260303128840314	No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT	6260	0.13218072864098787	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC	5957	0.1257828435326461	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTAAAA	5535	0.11687225767218336	No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5421	0.11446513258191615	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0061445035198925664	0.0	0.0	0.0	0.0
2	0.006228964049375625	0.0	0.0	0.0	2.1115132370764832E-5
3	0.00629230944648792	0.0	0.0	2.1115132370764832E-5	2.1115132370764832E-5
4	0.006313424578858685	0.0	0.0	2.1115132370764832E-5	2.1115132370764832E-5
5	0.006313424578858685	0.0	0.0	4.2230264741529664E-5	2.1115132370764832E-5
6	0.006313424578858685	0.0	0.0	4.2230264741529664E-5	2.1115132370764832E-5
7	0.00633453971122945	0.0	0.0	4.2230264741529664E-5	6.33453971122945E-5
8	0.00633453971122945	0.0	0.0	6.33453971122945E-5	6.33453971122945E-5
9	0.00633453971122945	0.0	0.0	8.446052948305933E-5	6.33453971122945E-5
10-11	0.00633453971122945	0.0	0.0	1.1613322803920658E-4	1.0557566185382417E-4
12-13	0.006376769975970979	0.0	0.0	1.26690794224589E-4	1.0557566185382417E-4
14-15	0.006461230505454039	0.0	0.0	1.26690794224589E-4	1.0557566185382417E-4
16-17	0.006524575902566334	0.0	0.0	1.4780592659535382E-4	1.2669079422458898E-4
18-19	0.00659847886586401	0.0	0.0	1.4780592659535382E-4	1.4780592659535382E-4
20-21	0.0067251696600886	0.0	0.0	1.4780592659535382E-4	1.6892105896611865E-4
22-23	0.006851860454313188	0.0	0.0	1.4780592659535382E-4	2.005937575222659E-4
24-25	0.006967993682352395	0.0	0.0	1.4780592659535382E-4	2.1115132370764833E-4
26-27	0.007189702572245426	0.0	0.0	1.4780592659535382E-4	2.1115132370764833E-4
28-29	0.007379738763582309	0.0	0.0	1.4780592659535382E-4	2.1115132370764833E-4
30-31	0.007485314425436132	0.0	0.0	1.4780592659535382E-4	2.1115132370764833E-4
32-33	0.007527544690177662	0.0	0.0	1.4780592659535382E-4	2.639391546345604E-4
34-35	0.007622562785846105	0.0	0.0	1.4780592659535382E-4	3.1672698556147247E-4
36-37	0.007728138447699928	1.0557566185382416E-5	0.0	1.4780592659535382E-4	3.1672698556147247E-4
38-39	0.007812598977182988	2.1115132370764832E-5	0.0	1.5836349278073623E-4	3.1672698556147247E-4
40-41	0.007918174639036813	2.1115132370764832E-5	0.0	1.6892105896611865E-4	3.1672698556147247E-4
42-43	0.00811876839655908	2.1115132370764832E-5	0.0	1.6892105896611865E-4	3.4839968411761975E-4
44-45	0.008319362154081345	2.1115132370764832E-5	0.0	1.6892105896611865E-4	3.80072382673767E-4
46-47	0.008604416441086669	2.1115132370764832E-5	0.0	1.6892105896611865E-4	4.011875150445318E-4
48-49	0.008900028294277378	2.1115132370764832E-5	0.0	1.6892105896611865E-4	4.011875150445318E-4
50-51	0.009280100676951144	2.1115132370764832E-5	0.0	1.6892105896611865E-4	4.011875150445318E-4
52-53	0.010188051368894032	2.1115132370764832E-5	0.0	1.6892105896611865E-4	4.011875150445318E-4
54-55	0.011349383649286098	2.1115132370764832E-5	0.0	1.6892105896611865E-4	4.011875150445318E-4
56-57	0.012563503760605076	2.1115132370764832E-5	0.0	1.6892105896611865E-4	4.011875150445318E-4
58-59	0.014020447894187849	2.1115132370764832E-5	0.0	1.6892105896611865E-4	4.011875150445318E-4
60-61	0.0160897308665228	2.1115132370764832E-5	0.0	1.6892105896611865E-4	4.2230264741529666E-4
62-63	0.018644661883385344	2.1115132370764832E-5	0.0	1.6892105896611865E-4	4.328602136006791E-4
64-65	0.02111513237076483	2.1115132370764832E-5	0.0	1.900361913368835E-4	4.434177797860615E-4
66-67	0.0249158561975025	2.1115132370764832E-5	0.0	1.900361913368835E-4	4.434177797860615E-4
68-69	0.02963508828236844	2.1115132370764832E-5	0.0	1.900361913368835E-4	4.434177797860615E-4
70-71	0.03438599306579053	2.1115132370764832E-5	0.0	1.900361913368835E-4	4.539753459714439E-4
72-73	0.04077332060794689	2.1115132370764832E-5	0.0	1.900361913368835E-4	4.856480445275911E-4
74-75	0.04844867122471991	3.1672698556147244E-5	0.0	1.900361913368835E-4	5.06763176898356E-4
76-77	0.057200893592401936	4.2230264741529664E-5	0.0	1.900361913368835E-4	5.173207430837384E-4
78-79	0.06947934306600168	4.2230264741529664E-5	0.0	1.900361913368835E-4	5.278783092691208E-4
80-81	0.08296135508473503	4.2230264741529664E-5	0.0	1.900361913368835E-4	5.595510078252681E-4
82-83	0.09930446753970701	4.2230264741529664E-5	0.0	1.900361913368835E-4	5.701085740106505E-4
84-85	0.11839254720287842	4.2230264741529664E-5	0.0	1.900361913368835E-4	5.806661401960328E-4
86-87	0.1430022339810048	4.2230264741529664E-5	0.0	1.900361913368835E-4	6.123388387521802E-4
88-89	0.17355583052150153	4.2230264741529664E-5	0.0	1.900361913368835E-4	6.123388387521802E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATGGA	149225	0.0	92.36199	1
TATGGAT	149535	0.0	91.44901	2
ATGGATC	149750	0.0	90.699196	3
ATATCTC	106040	0.0	90.57372	1
TATCTCG	106330	0.0	90.150276	2
TGGATCC	149580	0.0	89.865524	4
GGATCCG	149515	0.0	89.64726	5
ATCTCGA	108105	0.0	88.54266	3
TCTCGAG	108705	0.0	88.00152	4
GATCCGG	153195	0.0	87.74928	6
CTCGAGG	109775	0.0	87.299515	5
TCGAGGG	110025	0.0	87.11227	6
CGAGGGC	110450	0.0	86.73836	7
ATCCGGC	155040	0.0	86.689735	7
AGGGCGC	111995	0.0	86.088875	9
GAGGGCG	111985	0.0	85.96084	8
TCCGGCG	157110	0.0	85.84685	8
CCGGCGC	160425	0.0	84.59105	9
TATCGAT	400	0.0	68.87287	2
GTATCCG	420	0.0	64.46229	5
>>END_MODULE