FastQCFastQC Report
Tue 31 May 2016
SRR1051263_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051263_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4735940
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1561783.29771914340131No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304820.6436314649256536No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT180210.38051580045355304No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA106010.223841518262478No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT102530.2164934521974518No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91900.1940480664873288No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT82180.1735241578229454No Hit
CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69450.14664459431496177No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52100.11000983965168476No Hit
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51580.10891185276840501No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49610.10475217169136433No Hit
ATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT48150.10166936236523268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG17150.063.976272
ATATCTC21450.051.8193131
ATATGGA37600.049.396031
GGATCCG58400.039.282235
TCTCGAG32450.039.22634
TATGGAT49050.038.346632
ATCTCGA31000.038.304463
ATGGATC59150.036.2956623
TCGAGGG37950.033.666846
GATCCGG67700.033.605376
ATCCGGC71900.031.9726267
CTCGAGG40300.031.7036385
TCCGGCG80900.028.474438
TGGATCC80650.027.8556984
CGAGGGC48750.026.0134837
AGGGCGC51900.024.5261449
CCGGCGC102700.022.1527149
GGGCGCG48550.020.9356488
CGTCGCG1951.033566E-519.4876061
GAGGGCG73450.017.5889118
Produced by FastQC (version 0.11.5)