##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051263_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4735940 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.47135288876126 31.0 31.0 34.0 28.0 34.0 2 29.130714071546514 31.0 28.0 34.0 16.0 34.0 3 29.100160897308665 31.0 28.0 34.0 16.0 34.0 4 34.090006630151564 37.0 33.0 37.0 28.0 37.0 5 35.16307702377986 37.0 35.0 37.0 32.0 37.0 6 35.609286646367984 37.0 35.0 37.0 33.0 37.0 7 35.81074316819892 37.0 35.0 37.0 35.0 37.0 8 35.921338530471246 37.0 35.0 37.0 35.0 37.0 9 37.715973808789805 39.0 37.0 39.0 35.0 39.0 10-11 37.73744314750609 39.0 37.5 39.0 35.0 39.0 12-13 37.74752615109144 39.0 38.0 39.0 35.0 39.0 14-15 39.16788198330215 40.0 39.0 41.0 36.0 41.0 16-17 39.10776762374523 40.0 38.5 41.0 36.0 41.0 18-19 38.956933998319236 40.0 38.0 41.0 35.5 41.0 20-21 38.86098409185927 40.0 38.0 41.0 35.0 41.0 22-23 38.790222215653074 40.0 38.0 41.0 35.0 41.0 24-25 38.73504594652804 40.0 38.0 41.0 35.0 41.0 26-27 38.58405047361242 40.0 38.0 41.0 35.0 41.0 28-29 38.44528171809609 40.0 38.0 41.0 34.0 41.0 30-31 38.34480662761775 40.0 37.5 41.0 34.0 41.0 32-33 38.19634328137603 40.0 37.0 41.0 34.0 41.0 34-35 38.06053507856941 40.0 37.0 41.0 33.5 41.0 36-37 37.94632755060242 40.0 36.0 41.0 33.0 41.0 38-39 37.83486203372509 40.0 36.0 41.0 33.0 41.0 40-41 37.70250436027483 40.0 36.0 41.0 33.0 41.0 42-43 37.566397061618176 40.0 35.0 41.0 33.0 41.0 44-45 37.4043637588314 39.0 35.0 41.0 33.0 41.0 46-47 37.21167276190154 39.0 35.0 40.5 32.5 41.0 48-49 36.99586312326592 39.0 35.0 40.0 32.0 41.0 50-51 36.80807981097733 38.5 35.0 40.0 31.0 41.0 52-53 36.777657972862826 39.0 35.0 40.0 31.0 41.0 54-55 36.77202266076006 39.0 35.0 41.0 32.0 41.0 56-57 36.654703184584264 39.0 35.0 41.0 32.0 41.0 58-59 36.4109826138 38.0 35.0 40.5 31.0 41.0 60-61 36.10750706301178 37.5 35.0 40.0 31.0 41.0 62-63 35.77113012411475 37.0 35.0 40.0 31.0 41.0 64-65 35.41963137201907 36.5 35.0 40.0 30.5 41.0 66-67 35.04548188955097 36.0 34.5 39.0 30.0 41.0 68-69 34.6596203710351 35.0 34.0 39.0 30.0 41.0 70-71 34.3029204339582 35.0 34.0 38.5 29.0 40.0 72-73 33.89987468168938 35.0 34.0 37.0 29.0 40.0 74-75 33.518443434671894 35.0 34.0 37.0 29.0 39.0 76-77 32.74723148941921 35.0 33.0 36.0 27.0 39.0 78-79 32.829692310291094 35.0 33.0 36.0 28.0 38.0 80-81 32.61106717990515 35.0 33.0 35.5 27.0 37.0 82-83 32.34285717302161 35.0 33.0 35.0 27.0 37.0 84-85 32.058676946920784 35.0 33.0 35.0 26.0 36.0 86-87 31.78934298154115 35.0 33.0 35.0 25.5 36.0 88-89 31.52178511552089 35.0 33.0 35.0 24.5 36.0 90-91 31.26706461652808 35.0 33.0 35.0 24.0 35.5 92-93 31.019688594027798 35.0 33.0 35.0 21.5 35.0 94-95 30.758916286946203 35.0 33.0 35.0 19.0 35.0 96-97 30.484920522641758 35.0 33.0 35.0 8.5 35.0 98-99 30.194096314564796 35.0 32.0 35.0 2.0 35.0 100-101 29.64741033459039 34.5 31.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.1598932154448995 1101 2 3.6856039962058595 1101 3 3.776442712571704 1101 4 2.2237089770661598 1101 5 1.0586326057926385 1101 6 0.613652540037009 1101 7 0.46177198478572734 1101 8 0.3662451848042778 1101 9 0.3839130140140625 1101 10-11 0.351167708932806 1101 12-13 0.3106292566984976 1101 14-15 0.3754619009088529 1101 16-17 0.4186841745241594 1101 18-19 0.42468627736655407 1101 20-21 0.4357885499848422 1101 22-23 0.43032667504020594 1101 24-25 0.4492955570258914 1101 26-27 0.4608450454855131 1101 28-29 0.5056159661967925 1101 30-31 0.4759178363506038 1101 32-33 0.5254141487831134 1101 34-35 0.5614250579166296 1101 36-37 0.5457863203402269 1101 38-39 0.5071190146680777 1101 40-41 0.46645249034900615 1101 42-43 0.5235801037010006 1101 44-45 0.40301243409746235 1101 46-47 0.46151725292953216 1101 48-49 0.5278461772930143 1101 50-51 0.4672538631042755 1101 52-53 0.436681005658329 1101 54-55 0.5127781850838673 1101 56-57 0.41788148323588103 1101 58-59 0.49084835636143964 1101 60-61 0.456098278437544 1101 62-63 0.42966888504506784 1101 64-65 0.46524500207980424 1101 66-67 0.46049887211048457 1101 68-69 0.44791856828621945 1101 70-71 0.44526204529939406 1101 72-73 0.4296596293112742 1101 74-75 0.44345938370511107 1101 76-77 0.43302764784125714 1101 78-79 0.3941980924377475 1101 80-81 0.3825688645454548 1101 82-83 0.3209650975452618 1101 84-85 0.39284720840635146 1101 86-87 0.42973108088961354 1101 88-89 0.40615330198688326 1101 90-91 0.4201906501239101 1101 92-93 0.38938120935715403 1101 94-95 0.4028349047052515 1101 96-97 0.44968540824358527 1101 98-99 0.5460847248070522 1101 100-101 0.5118854892100764 1102 1 1.1016131667282743 1102 2 3.613197892018494 1102 3 3.712602078083325 1102 4 2.1715140913513906 1102 5 1.1080449587143306 1102 6 0.6173049830734598 1102 7 0.441581241776106 1102 8 0.3257027223992779 1102 9 0.2973424854556086 1102 10-11 0.30863747412313813 1102 12-13 0.29152085363305247 1102 14-15 0.31715009973246566 1102 16-17 0.24200356052983807 1102 18-19 0.26566500785325076 1102 20-21 0.31857809194460174 1102 22-23 0.3601405060889249 1102 24-25 0.40646465179069935 1102 26-27 0.4339928700915152 1102 28-29 0.5143030815766494 1102 30-31 0.48568861496274707 1102 32-33 0.32987640799922247 1102 34-35 0.4941798871242895 1102 36-37 0.5294080543134498 1102 38-39 0.4958981833916525 1102 40-41 0.49626830826642276 1102 42-43 0.4383449535950703 1102 44-45 0.46611475127694746 1102 46-47 0.4133249264668919 1102 48-49 0.4756662415064383 1102 50-51 0.44447407784463167 1102 52-53 0.3458865478833104 1102 54-55 0.3837959237804469 1102 56-57 0.4210183022159768 1102 58-59 0.45757667792629064 1102 60-61 0.4182669751830943 1102 62-63 0.3971982155002678 1102 64-65 0.4278551993819377 1102 66-67 0.454724016944688 1102 68-69 0.4271486833906053 1102 70-71 0.42054655418120745 1102 72-73 0.36513093018714926 1102 74-75 0.31641431292494104 1102 76-77 0.2600026075894135 1102 78-79 0.26705338128985545 1102 80-81 0.2016235382402911 1102 82-83 0.31924136953305293 1102 84-85 0.3100274399639531 1102 86-87 0.3604973566298675 1102 88-89 0.3482520106048561 1102 90-91 0.38129782450666383 1102 92-93 0.3239134275239621 1102 94-95 0.3231165492169765 1102 96-97 0.34926125373496575 1102 98-99 0.30809066615123015 1102 100-101 0.37209824476251185 1103 1 0.9894976431477751 1103 2 3.4845758318129505 1103 3 3.6118471966270924 1103 4 2.142891502802499 1103 5 1.0136168676004047 1103 6 0.5415417596090464 1103 7 0.39731241952030416 1103 8 0.2874742459921791 1103 9 0.2559867496536441 1103 10-11 0.20694252929285284 1103 12-13 0.13369524532566857 1103 14-15 0.28653008251912837 1103 16-17 0.2740632406426613 1103 18-19 0.26158411296177775 1103 20-21 0.2969205591548345 1103 22-23 0.35337853533142294 1103 24-25 0.2569917154755501 1103 26-27 0.27619938651348974 1103 28-29 0.3747182969148568 1103 30-31 0.2986715038122938 1103 32-33 0.3356438330050082 1103 34-35 0.42260327569163536 1103 36-37 0.426016254861338 1103 38-39 0.4854057450997402 1103 40-41 0.4763305330552825 1103 42-43 0.3909326581051431 1103 44-45 0.40162648379523347 1103 46-47 0.41214479405726223 1103 48-49 0.4125880569678415 1103 50-51 0.4383764503679899 1103 52-53 0.29030445263454396 1103 54-55 0.3530433117769931 1103 56-57 0.30926694776662345 1103 58-59 0.339604262392875 1103 60-61 0.3511925882213305 1103 62-63 0.3431866693078405 1103 64-65 0.29890763847654966 1103 66-67 0.3169320975537886 1103 68-69 0.2996009899825296 1103 70-71 0.3188992087235789 1103 72-73 0.3054509870116888 1103 74-75 0.25279632144785324 1103 76-77 0.1739757936316053 1103 78-79 0.2200871723850355 1103 80-81 0.21117398640146945 1103 82-83 0.24535153522733566 1103 84-85 0.3421044366509989 1103 86-87 0.3028489299587811 1103 88-89 0.38864316838640534 1103 90-91 0.43366520499588646 1103 92-93 0.3681156009636659 1103 94-95 0.3926342938947194 1103 96-97 0.3657888513859042 1103 98-99 0.3420737462707635 1103 100-101 0.42311832549337325 1104 1 0.7829618984949072 1104 2 2.02880366873217 1104 3 2.9423055304139147 1104 4 1.862036479433506 1104 5 0.8452262461041684 1104 6 0.39746386859946625 1104 7 0.3167984077933994 1104 8 0.12633541759096545 1104 9 0.14560729099973457 1104 10-11 0.12293985387338324 1104 12-13 0.1240968721649196 1104 14-15 0.14341515452581888 1104 16-17 0.11855210051226095 1104 18-19 0.11823244128785149 1104 20-21 0.18408032556474296 1104 22-23 0.1547449880143006 1104 24-25 0.16267827709650362 1104 26-27 0.2086613874437333 1104 28-29 0.24724569378945915 1104 30-31 0.21879745109059456 1104 32-33 0.14060232235725323 1104 34-35 0.16612977070054313 1104 36-37 0.250763979374085 1104 38-39 0.22701347620208878 1104 40-41 0.2938149904548695 1104 42-43 0.32571716109048054 1104 44-45 0.22754216890958645 1104 46-47 0.23525726578991168 1104 48-49 0.32658236990251766 1104 50-51 0.32542279208896474 1104 52-53 0.3150241594051906 1104 54-55 0.27229558087792327 1104 56-57 0.2706989106280844 1104 58-59 0.24679001888743102 1104 60-61 0.25906215347196593 1104 62-63 0.235375884086082 1104 64-65 0.2796525663646179 1104 66-67 0.27020878255579817 1104 68-69 0.3031053777460002 1104 70-71 0.319708495228312 1104 72-73 0.3118376357654711 1104 74-75 0.3435477995892171 1104 76-77 0.23868754133516745 1104 78-79 0.20574782037731154 1104 80-81 0.21818820941671646 1104 82-83 0.2276356287234691 1104 84-85 0.23007100474642783 1104 86-87 0.23264502344519045 1104 88-89 0.16864698376447151 1104 90-91 0.15853287769126823 1104 92-93 0.18695534734929709 1104 94-95 0.16605283801282766 1104 96-97 0.1964532340277323 1104 98-99 0.32042071117989934 1104 100-101 0.36524762801968436 1105 1 0.14828058266807886 1105 2 -1.2215349060744067 1105 3 0.10218404975830708 1105 4 0.5186884589638652 1105 5 0.20513548367951984 1105 6 0.10837817311785614 1105 7 0.06748528548953914 1105 8 -0.007227152613367593 1105 9 0.004363533668701791 1105 10-11 -0.07970409543802504 1105 12-13 -0.059598277606930594 1105 14-15 -0.07207252815604903 1105 16-17 -0.12081118675523328 1105 18-19 -0.1809934380670697 1105 20-21 -0.13916960409827084 1105 22-23 -0.16292428615021493 1105 24-25 0.008702077808251829 1105 26-27 -0.03473301373846027 1105 28-29 -0.05005255654272389 1105 30-31 -0.07908319786248086 1105 32-33 -0.022749626435583536 1105 34-35 -0.0192362188622468 1105 36-37 -0.09167739984103207 1105 38-39 -0.2204326913737944 1105 40-41 -0.2081006111198178 1105 42-43 -0.04103622504885607 1105 44-45 -0.12479490574620655 1105 46-47 -0.07040152417034307 1105 48-49 -0.05353506537132802 1105 50-51 -0.03426475775071225 1105 52-53 -0.026392448005033486 1105 54-55 -0.11903308680606273 1105 56-57 -0.1757250792983669 1105 58-59 -0.20250832744994796 1105 60-61 -0.2438494322857352 1105 62-63 -0.231592386026648 1105 64-65 -0.1071543221943898 1105 66-67 -0.15867598829591145 1105 68-69 -0.11763603360019914 1105 70-71 -0.10267285651577396 1105 72-73 -0.12057867576096015 1105 74-75 -0.10914634448644733 1105 76-77 -0.10910665792311391 1105 78-79 -0.08121072122364126 1105 80-81 -0.09881840274253051 1105 82-83 -0.07039846130929561 1105 84-85 -0.06364271767778718 1105 86-87 -0.04484056285891924 1105 88-89 0.03297799684370872 1105 90-91 0.06218303880440246 1105 92-93 -0.02144899384337151 1105 94-95 -0.12759447617547792 1105 96-97 -0.0756825819738367 1105 98-99 -0.0017427604484332448 1105 100-101 -0.03933502478555795 1106 1 -0.48154997927316856 1106 2 -3.5514956513722993 1106 3 -3.530271224421057 1106 4 -1.3296457996505637 1106 5 -0.6675003311199816 1106 6 -0.41061555182842113 1106 7 -0.21661557791583874 1106 8 -0.19174767205770848 1106 9 -0.1996442255418529 1106 10-11 -0.23532964485326602 1106 12-13 -0.19904823851620534 1106 14-15 -0.25724914955659983 1106 16-17 -0.23645647513973245 1106 18-19 -0.22963792020284046 1106 20-21 -0.27088221132988366 1106 22-23 -0.24067169317813608 1106 24-25 -0.17310550028044958 1106 26-27 -0.3001386903075485 1106 28-29 -0.32507462853541114 1106 30-31 -0.3047655990972089 1106 32-33 -0.31167607969851474 1106 34-35 -0.3491312717729258 1106 36-37 -0.3538647887048114 1106 38-39 -0.27993261218574617 1106 40-41 -0.2620474552023353 1106 42-43 -0.2756851500762494 1106 44-45 -0.20327422378575477 1106 46-47 -0.19642017220356678 1106 48-49 -0.2296765989600047 1106 50-51 -0.2550863992461885 1106 52-53 -0.1457883719030093 1106 54-55 -0.16812106865881304 1106 56-57 -0.1787544785594406 1106 58-59 -0.18046706021072367 1106 60-61 -0.22859446058765087 1106 62-63 -0.22151785875409757 1106 64-65 -0.14629738580482865 1106 66-67 -0.16102713446064598 1106 68-69 -0.1927589719206324 1106 70-71 -0.23319165630916672 1106 72-73 -0.27466554792579956 1106 74-75 -0.21462314336400112 1106 76-77 -0.23680979717092754 1106 78-79 -0.15510584914176206 1106 80-81 -0.0954952649542733 1106 82-83 -0.14935215672017677 1106 84-85 -0.1853066423248606 1106 86-87 -0.15779922134284874 1106 88-89 -0.18002645351618085 1106 90-91 -0.16982115373773254 1106 92-93 -0.20904873485666897 1106 94-95 -0.19993702530311808 1106 96-97 -0.19689227126203335 1106 98-99 -0.18297296800390228 1106 100-101 -0.23056801954908224 1107 1 -0.780623640952669 1107 2 -2.44869228230176 1107 3 -4.5614337206916 1107 4 -2.053545433124995 1107 5 -1.036532245483393 1107 6 -0.6827102894634152 1107 7 -0.38890712012584316 1107 8 -0.4214567595861496 1107 9 -0.3373988701833568 1107 10-11 -0.37542916418760086 1107 12-13 -0.32763307100329087 1107 14-15 -0.5676784141024669 1107 16-17 -0.5051741536366947 1107 18-19 -0.46327379679129876 1107 20-21 -0.5086067790237152 1107 22-23 -0.4839510052522158 1107 24-25 -0.4921614916589476 1107 26-27 -0.5341631393448409 1107 28-29 -0.5901745060184069 1107 30-31 -0.5584446812323094 1107 32-33 -0.6130852382308802 1107 34-35 -0.5794815585067496 1107 36-37 -0.494153976030816 1107 38-39 -0.465905461576547 1107 40-41 -0.5583536644671625 1107 42-43 -0.620329553317255 1107 44-45 -0.6487307555087938 1107 46-47 -0.6157846125201232 1107 48-49 -0.534138315092278 1107 50-51 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2304 22-23 0.22910371937256713 2304 24-25 0.19123582312549559 2304 26-27 0.2213581276502694 2304 28-29 0.25103392217310017 2304 30-31 0.2174071211750359 2304 32-33 0.24130205566127927 2304 34-35 0.2393086312335626 2304 36-37 0.3463852209980445 2304 38-39 0.3877369596707254 2304 40-41 0.2391610559246118 2304 42-43 0.1950440115944403 2304 44-45 0.21946241369963815 2304 46-47 0.2628069361591088 2304 48-49 0.22874345645723793 2304 50-51 0.23796393262971804 2304 52-53 0.17752107549456753 2304 54-55 0.2522377869177248 2304 56-57 0.13353526581959585 2304 58-59 0.20307247571887643 2304 60-61 0.21557055062000785 2304 62-63 0.26798251338581736 2304 64-65 0.223731461996465 2304 66-67 0.15217336336275622 2304 68-69 0.12758535605477306 2304 70-71 0.048624686142211715 2304 72-73 0.013199917223168711 2304 74-75 0.04776751265679735 2304 76-77 0.13352731180098232 2304 78-79 0.07538461761799908 2304 80-81 0.07861059083752053 2304 82-83 0.09390186847949877 2304 84-85 0.09975421042416599 2304 86-87 0.1133062511383578 2304 88-89 0.0742918144475837 2304 90-91 0.10412921039970868 2304 92-93 0.09861322225692248 2304 94-95 0.1258864062211167 2304 96-97 0.11089049502654902 2304 98-99 0.18774671765243056 2304 100-101 0.14623571147286896 2305 1 0.05553984900118536 2305 2 -2.068687617030026 2305 3 -0.904027622106998 2305 4 0.09513875841872732 2305 5 -0.00300130593921466 2305 6 -0.01774242150403893 2305 7 0.002423355673208505 2305 8 0.03431368602073093 2305 9 0.0545276601255793 2305 10-11 -0.012994704078977293 2305 12-13 0.005881759258706154 2305 14-15 0.0579164181903451 2305 16-17 0.03276387927531488 2305 18-19 -0.01970566199933188 2305 20-21 -0.021465572605244176 2305 22-23 0.007875129840606121 2305 24-25 0.01831186627812542 2305 26-27 -0.008651281293367674 2305 28-29 0.016245004189308077 2305 30-31 0.057595928032107224 2305 32-33 0.0434360213172198 2305 34-35 -0.094080687727363 2305 36-37 -0.11747785521533416 2305 38-39 -0.12194956758094122 2305 40-41 0.01975734287695019 2305 42-43 0.027656922929288896 2305 44-45 0.09474101876703145 2305 46-47 0.13074347004323528 2305 48-49 0.09615877829657649 2305 50-51 0.01959383771229284 2305 52-53 0.07768700999173461 2305 54-55 0.016146818298402366 2305 56-57 0.039281324042725885 2305 58-59 0.025858692064204547 2305 60-61 0.004421165094996127 2305 62-63 -0.015600812798894026 2305 64-65 -0.015621452233155253 2305 66-67 0.02123627645692494 2305 68-69 -0.039234690398153305 2305 70-71 -0.023095458754625042 2305 72-73 0.026901616769585246 2305 74-75 0.023201871263744067 2305 76-77 0.05805457824128979 2305 78-79 0.021858620094036496 2305 80-81 0.012351148171909188 2305 82-83 -0.07636818305363846 2305 84-85 -0.07565222470969957 2305 86-87 -0.03624113934433737 2305 88-89 -0.03682442932718999 2305 90-91 -0.08404791796427702 2305 92-93 -0.14107835760045973 2305 94-95 -0.1155893785288491 2305 96-97 -0.04667219342237772 2305 98-99 -0.04282132866303101 2305 100-101 -0.06667707673153345 2306 1 -0.6665386047916577 2306 2 -2.3323079112086518 2306 3 -4.361081203987222 2306 4 -1.8801141700518471 2306 5 -0.8889238616782933 2306 6 -0.520910659457904 2306 7 -0.3613420391732518 2306 8 -0.29455347190314285 2306 9 -0.2030106378010501 2306 10-11 -0.24258502523791492 2306 12-13 -0.2194786061088294 2306 14-15 -0.22318314749912815 2306 16-17 -0.31441339371913557 2306 18-19 -0.250879435818554 2306 20-21 -0.2055850950292708 2306 22-23 -0.20500127776664812 2306 24-25 -0.29618380984209125 2306 26-27 -0.2251145513963877 2306 28-29 -0.30073573986357616 2306 30-31 -0.38379640511171687 2306 32-33 -0.3114077666961421 2306 34-35 -0.35193295406040903 2306 36-37 -0.3103900033370621 2306 38-39 -0.2635669895971944 2306 40-41 -0.3203476096361726 2306 42-43 -0.3323988827783282 2306 44-45 -0.3493976228581701 2306 46-47 -0.2805542628383506 2306 48-49 -0.3416230869735486 2306 50-51 -0.33611859622552487 2306 52-53 -0.17750892726063228 2306 54-55 -0.30548935582297077 2306 56-57 -0.3547631785890246 2306 58-59 -0.3485935778793987 2306 60-61 -0.26585000872466225 2306 62-63 -0.22736480919773072 2306 64-65 -0.2842676751914439 2306 66-67 -0.23180252726623252 2306 68-69 -0.23914487802084494 2306 70-71 -0.32309521177324996 2306 72-73 -0.2731799954708052 2306 74-75 -0.2656602923058742 2306 76-77 -0.1828685897983675 2306 78-79 -0.21156958725403996 2306 80-81 -0.23008975593769776 2306 82-83 -0.23277974485225883 2306 84-85 -0.2697652175859062 2306 86-87 -0.3024387019118251 2306 88-89 -0.2808757052898194 2306 90-91 -0.3308013300563566 2306 92-93 -0.3473168209939459 2306 94-95 -0.3995242637201777 2306 96-97 -0.3369732811283441 2306 98-99 -0.3890904209450099 2306 100-101 -0.4030758694155132 2307 1 -4.089034575204671 2307 2 -4.665116530651289 2307 3 -5.062845523517133 2307 4 -8.020274576681246 2307 5 -4.217566343848013 2307 6 -2.0476566523321083 2307 7 -1.0762569184583697 2307 8 -0.7609898685790668 2307 9 -0.5994892881548424 2307 10-11 -0.4989587774280153 2307 12-13 -0.4187948975679916 2307 14-15 -0.48096391979198216 2307 16-17 -0.4398232970180658 2307 18-19 -0.46303745909114014 2307 20-21 -0.6942615774904013 2307 22-23 -0.7049493731887466 2307 24-25 -0.7857514462120179 2307 26-27 -0.7880844290816214 2307 28-29 -0.8628129597821967 2307 30-31 -0.744827118313701 2307 32-33 -0.6793134625231971 2307 34-35 -0.7090595342443677 2307 36-37 -0.6909730861742389 2307 38-39 -0.7662892676220849 2307 40-41 -0.7850916184492931 2307 42-43 -0.7092422096263036 2307 44-45 -0.6567602593047965 2307 46-47 -0.6565422007386488 2307 48-49 -0.7565048051199241 2307 50-51 -0.6537493266917878 2307 52-53 -0.7179170171218985 2307 54-55 -0.7048648391252712 2307 56-57 -0.7675312783018384 2307 58-59 -0.7244580812795576 2307 60-61 -0.7720080862220655 2307 62-63 -0.7588044067275703 2307 64-65 -0.8080829284945992 2307 66-67 -0.6688153498453389 2307 68-69 -0.7336324872422821 2307 70-71 -0.745526639831894 2307 72-73 -0.6003390132230706 2307 74-75 -0.5357129041266191 2307 76-77 -0.739712782009093 2307 78-79 -0.8425690292368557 2307 80-81 -0.8615120343965614 2307 82-83 -0.7865262907594293 2307 84-85 -0.6822991558968852 2307 86-87 -0.6643399070905751 2307 88-89 -0.7092440524431041 2307 90-91 -0.9552821231497113 2307 92-93 -0.9408210436333064 2307 94-95 -1.0213422456229289 2307 96-97 -1.0573526962883548 2307 98-99 -1.0655449747703791 2307 100-101 -1.0987201536795261 2308 1 -3.658829239931496 2308 2 -4.709546124305824 2308 3 -4.4352536018782835 2308 4 -3.9516931603167684 2308 5 -1.7249691567516194 2308 6 -1.0104200342148957 2308 7 -0.674642139098026 2308 8 -0.4913447139518894 2308 9 -0.5182562352684599 2308 10-11 -0.5008886056885871 2308 12-13 -0.4797403462303933 2308 14-15 -0.967679061727658 2308 16-17 -0.7347251939494157 2308 18-19 -0.7849363089423633 2308 20-21 -0.768828506460288 2308 22-23 -0.8442827965899724 2308 24-25 -0.8306996591257558 2308 26-27 -0.8219942620095821 2308 28-29 -0.8229641580040621 2308 30-31 -0.862914039817845 2308 32-33 -0.8895534531103237 2308 34-35 -0.8630824258064038 2308 36-37 -0.9484875923763596 2308 38-39 -0.9692132072385959 2308 40-41 -0.8647887274130923 2308 42-43 -0.9629526814375922 2308 44-45 -0.9172646551255994 2308 46-47 -0.8663580522453245 2308 48-49 -1.206130112271616 2308 50-51 -0.9517831458841428 2308 52-53 -0.8340548733212216 2308 54-55 -0.9228505578976467 2308 56-57 -0.8166869633885696 2308 58-59 -0.9471772387855211 2308 60-61 -0.9344789622091199 2308 62-63 -0.8927032414807101 2308 64-65 -0.9027173940672526 2308 66-67 -1.031269168899719 2308 68-69 -1.011697375953112 2308 70-71 -0.889434295251398 2308 72-73 -0.9415042054184539 2308 74-75 -1.1168239813971894 2308 76-77 -0.7770572343019388 2308 78-79 -0.7919795075429263 2308 80-81 -0.8036687283217816 2308 82-83 -0.7814572905533907 2308 84-85 -0.779767519223995 2308 86-87 -0.8182964581826973 2308 88-89 -0.8558216677342365 2308 90-91 -0.9065076647382391 2308 92-93 -0.9909993442213647 2308 94-95 -0.8929005360145688 2308 96-97 -1.0524239260652202 2308 98-99 -1.1235918512041323 2308 100-101 -1.162472499251809 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 21.0 11 293.0 12 800.0 13 1804.0 14 3036.0 15 4610.0 16 6306.0 17 9146.0 18 15446.0 19 29769.0 20 28668.0 21 25518.0 22 25487.0 23 26799.0 24 30016.0 25 34682.0 26 41617.0 27 50242.0 28 61815.0 29 75873.0 30 94032.0 31 115615.0 32 147420.0 33 194443.0 34 274373.0 35 488844.0 36 686763.0 37 1111293.0 38 975099.0 39 175923.0 40 187.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 23.912526575503996 30.466022632151102 24.641668869853728 20.979781922491174 2 23.54913579913242 28.226289375532104 29.276810328517954 18.947764496817523 3 24.431839417312993 29.25508878759539 25.950530981430376 20.362540813661234 4 23.851336799030392 27.976262368188788 27.114617161534987 21.057783671245836 5 24.25854212680059 28.224956397251656 24.85365101753823 22.662850458409523 6 23.827962347495955 27.71943901316317 25.197257566607682 23.255341072733184 7 23.753890463139314 28.256481289881208 25.228275696060336 22.761352550919142 8 23.12493401521134 27.62268947664033 25.839136475546564 23.413240032601763 9 22.683754439456578 27.426044248871396 26.20512506492903 23.68507624674299 10-11 23.752989509044305 27.794407716404134 25.43996314139769 23.012639633153864 12-13 23.11686734903952 28.04581998236881 25.753110532789265 23.084202135802403 14-15 23.018566535893616 28.393159541717168 25.953316553841475 22.63495736854774 16-17 23.05119997297263 28.347529740663944 25.89159702192173 22.709673264441697 18-19 22.666228879588846 28.4875336258483 26.410691436124612 22.43554605843824 20-21 22.176727323403593 28.535992854639208 27.196607220530666 22.09067260142654 22-23 22.310069384324972 28.70309801222144 27.102317596929016 21.884515006524577 24-25 21.90829064557406 28.437575222659074 27.874698581485408 21.779435550281466 26-27 21.776876167511556 28.613553529537043 28.292735001446072 21.31683530150532 28-29 21.970792623530482 28.792069435830708 28.000731894841113 21.236406045797697 30-31 21.916232139844258 28.75750351960919 28.122654077794817 21.203610262751734 32-33 21.70810426347119 29.065346390799434 28.05954376864861 21.167005577080772 34-35 21.846787610386812 29.21875084137294 27.842779428467175 21.091682119773075 36-37 21.742197053990743 29.24016502855183 27.9341570964199 21.08348082103753 38-39 21.61685828932618 29.51011480044588 27.867260809880857 21.00576610034708 40-41 21.733862780832574 29.702192644545494 27.593391246800714 20.970553327821218 42-43 21.54116281900674 29.775774792303224 27.697006955655283 20.986055433034753 44-45 21.44762180264108 30.116544973120433 27.557105875496735 20.878727348741748 46-47 21.553143221047236 30.342880333315176 27.229241599024313 20.87473484661328 48-49 21.414927240726232 30.518649417099148 27.256330362460307 20.810092979714316 50-51 21.336961415049004 30.85302151510314 27.123225973196707 20.68679109665115 52-53 21.405077070769167 31.092933091733965 26.83715659796752 20.664833239529347 54-55 21.319790040601372 31.22446577073713 26.816627610304966 20.63911657835653 56-57 21.186776014898836 31.646779731162134 26.63238976845146 20.53405448548757 58-59 21.306304080571685 31.872922982973368 26.262416702880508 20.558356233574436 60-61 21.114403898698043 32.04739713763266 26.286122712703285 20.552076250966017 62-63 21.091377846847724 32.39356917528516 26.116399278707082 20.39865369916004 64-65 21.099927722184603 32.609911161421934 25.839994695826107 20.450166420567356 66-67 20.953433798991473 32.78721201305725 25.81038483916566 20.448969348785614 68-69 20.84228263026981 33.198541366655824 25.611272524567458 20.347903478506908 70-71 20.95397112294497 33.447288183549624 25.339721364713235 20.25901932879217 72-73 20.80976532641883 33.62958567887262 25.315375617089742 20.245273377618805 74-75 20.66661528651123 34.05826509626389 25.095440398315855 20.179679218909026 76-77 20.78257959349147 34.367654573326526 24.75785166197207 20.09191417120994 78-79 20.57502839985304 34.56860728809909 24.73007470533833 20.126289606709545 80-81 20.394494018082998 35.00125635037606 24.557574631435365 20.046675000105576 82-83 20.52070972182925 35.31849009911443 24.142387783629015 20.01841239542731 84-85 20.362715743865 35.50324750735862 24.108487438607753 20.025549310168625 86-87 20.313338006815965 35.87662639307086 23.90257266772805 19.907462932385123 88-89 20.333777454950866 36.185963082302564 23.56175331613154 19.918506146615034 90-91 20.25003483996841 36.31206265282077 23.476258145162312 19.961644362048506 92-93 20.169501725106315 36.67086153963099 23.266711571514843 19.89292516374785 94-95 20.245041111162728 36.911120073311736 22.942319792902783 19.90151902262275 96-97 20.09907220108363 37.086502362783314 22.86069924872359 19.95372618740947 98-99 20.102197240674503 37.32356195390989 22.670304100136406 19.903936705279204 100-101 20.15952482506113 37.535652901008035 22.299902448088446 20.00491982584239 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 150633.0 1 86743.0 2 16266.5 3 8321.5 4 6826.0 5 6775.5 6 7077.5 7 7539.0 8 8067.0 9 8390.5 10 9190.5 11 10459.0 12 11617.0 13 13954.0 14 18977.0 15 22870.5 16 29217.5 17 29115.5 18 22552.0 19 22367.0 20 23843.0 21 25714.5 22 27315.5 23 29554.0 24 32003.0 25 34399.5 26 37666.0 27 41390.5 28 45518.5 29 51519.5 30 58957.0 31 64819.5 32 71782.0 33 79422.5 34 83680.0 35 88277.5 36 96543.0 37 103463.0 38 106170.5 39 107867.5 40 108318.5 41 107752.5 42 107968.5 43 109972.0 44 113368.5 45 119109.5 46 127671.5 47 137731.5 48 147511.5 49 155408.5 50 157628.0 51 153587.0 52 149493.0 53 148352.5 54 147407.5 55 143200.0 56 135152.5 57 126155.0 58 116711.5 59 106321.0 60 94597.5 61 81967.5 62 70617.5 63 58733.0 64 45120.0 65 32413.5 66 23659.0 67 18484.0 68 14356.0 69 10870.5 70 8537.5 71 6339.5 72 4685.5 73 3748.5 74 3175.0 75 2796.5 76 2362.0 77 1545.5 78 806.5 79 413.5 80 190.0 81 83.5 82 37.5 83 16.0 84 5.0 85 3.5 86 2.5 87 1.0 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007939289771407577 2 8.446052948305933E-5 3 0.0018370165162565402 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.390296329767691E-5 12-13 3.167269855614725E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.014960071284686884 28-29 0.006060042990409507 30-31 0.00694687854998163 32-33 0.014083793291300143 34-35 0.007949847337592958 36-37 0.01091652343568542 38-39 0.0033150757822100786 40-41 0.0065773637334932454 42-43 2.0059375752226588E-4 44-45 0.0 46-47 4.2230264741529664E-5 48-49 3.061694193760901E-4 50-51 0.0021959737665595423 52-53 1.0557566185382416E-5 54-55 0.005542722247325769 56-57 0.0 58-59 0.0038429540914791996 60-61 0.0 62-63 0.0 64-65 9.924112214259471E-4 66-67 5.59551007825268E-4 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4735940.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.045170007944975 #Duplication Level Percentage of deduplicated Percentage of total 1 79.9886705892168 39.23057947757646 2 9.716401157234165 9.530850932438858 3 3.1149188144262254 4.583151684434419 4 1.5728612562761626 3.0856499085189717 5 1.0235955304748068 2.510120840575476 6 0.7188877592745181 2.1154783422149617 7 0.5475284237473996 1.8797537238811346 8 0.42280277541070116 1.6589147199879055 9 0.3441234656934622 1.5189834490793166 >10 2.4001413887920666 21.862164999567252 >50 0.12947673323474665 4.157406369660936 >100 0.018768455188861173 1.3167999348401687 >500 6.078838687872135E-4 0.20124291044678186 >1k 7.815646036909059E-4 0.8066111025100556 >5k 2.1710127880297635E-4 0.739402383775065 >10k+ 2.1710127880297635E-4 4.8028892204921885 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 156178 3.29771914340131 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30482 0.6436314649256536 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 18021 0.38051580045355304 No Hit ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10601 0.223841518262478 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 10253 0.2164934521974518 No Hit GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9190 0.1940480664873288 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8218 0.1735241578229454 No Hit CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6945 0.14664459431496177 No Hit AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5210 0.11000983965168476 No Hit CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5158 0.10891185276840501 No Hit ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4961 0.10475217169136433 No Hit ATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4815 0.10166936236523268 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0027449672081994283 0.0 0.0 0.0 0.0 2 0.002787197472940958 0.0 0.0 0.0 0.0 3 0.002787197472940958 0.0 0.0 0.0 0.0 4 0.0028083126053117225 0.0 0.0 0.0 0.0 5 0.0028083126053117225 0.0 0.0 0.0 0.0 6 0.0028083126053117225 0.0 0.0 0.0 6.33453971122945E-5 7 0.0028083126053117225 0.0 0.0 0.0 6.33453971122945E-5 8 0.0028083126053117225 0.0 0.0 0.0 6.33453971122945E-5 9 0.0028083126053117225 0.0 0.0 0.0 6.33453971122945E-5 10-11 0.0028083126053117225 0.0 0.0 0.0 9.501809566844175E-5 12-13 0.0028505428700532523 0.0 0.0 0.0 1.5836349278073623E-4 14-15 0.0029244458333509294 0.0 0.0 0.0 1.900361913368835E-4 16-17 0.002977233664277841 0.0 0.0 0.0 2.005937575222659E-4 18-19 0.0030300214952047534 0.0 0.0 0.0 2.2170888989303072E-4 20-21 0.0031461547232439603 0.0 0.0 0.0 2.53381588449178E-4 22-23 0.0032939606498393137 0.0 0.0 0.0 2.7449672081994285E-4 24-25 0.003462881708805433 0.0 0.0 0.0 2.9561185319070763E-4 26-27 0.003684590598698463 0.0 0.0 0.0 2.9561185319070763E-4 28-29 0.003853511657664582 0.0 0.0 2.1115132370764832E-5 3.1672698556147247E-4 30-31 0.003980202451889171 0.0 0.0 2.1115132370764832E-5 3.1672698556147247E-4 32-33 0.0040224327166307 2.1115132370764832E-5 0.0 2.1115132370764832E-5 3.80072382673767E-4 34-35 0.004096335679928377 2.1115132370764832E-5 0.0 2.1115132370764832E-5 4.4341777978606145E-4 36-37 0.004138565944669907 2.1115132370764832E-5 0.0 2.1115132370764832E-5 4.962056107129736E-4 38-39 0.0042019113417822015 2.1115132370764832E-5 0.0 2.1115132370764832E-5 5.173207430837384E-4 40-41 0.004265256738894496 2.1115132370764832E-5 0.0 2.1115132370764832E-5 5.489934416398857E-4 42-43 0.004339159702192173 2.1115132370764832E-5 0.0 2.1115132370764832E-5 6.123388387521802E-4 44-45 0.0044236202316752325 2.1115132370764832E-5 0.0 2.1115132370764832E-5 6.334539711229449E-4 46-47 0.004581983724455969 2.1115132370764832E-5 0.0 2.1115132370764832E-5 6.545691034937097E-4 48-49 0.004772019915792852 2.1115132370764832E-5 0.0 2.1115132370764832E-5 6.967993682352395E-4 50-51 0.005004286371871265 2.1115132370764832E-5 0.0 2.1115132370764832E-5 7.812598977182989E-4 52-53 0.0059227946299995355 2.1115132370764832E-5 0.0 2.1115132370764832E-5 8.12932596274446E-4 54-55 0.007063011778020836 2.1115132370764832E-5 0.0 2.1115132370764832E-5 8.234901624598284E-4 56-57 0.008182113793671373 2.1115132370764832E-5 0.0 2.1115132370764832E-5 8.234901624598284E-4 58-59 0.00966017305962491 2.1115132370764832E-5 0.0 2.1115132370764832E-5 8.234901624598284E-4 60-61 0.011729456031959865 2.1115132370764832E-5 0.0 2.1115132370764832E-5 8.446052948305933E-4 62-63 0.014210484085524732 2.1115132370764832E-5 0.0 2.1115132370764832E-5 8.446052948305933E-4 64-65 0.016712627271460365 4.2230264741529664E-5 0.0 2.1115132370764832E-5 8.446052948305933E-4 66-67 0.020460563267271123 4.2230264741529664E-5 0.0 2.1115132370764832E-5 8.657204272013582E-4 68-69 0.02517979535213706 4.2230264741529664E-5 0.0 2.1115132370764832E-5 9.501809566844175E-4 70-71 0.029835682039890708 4.2230264741529664E-5 0.0 2.1115132370764832E-5 9.712960890551822E-4 72-73 0.03603297339071018 4.2230264741529664E-5 0.0 2.1115132370764832E-5 0.0010135263537967118 74-75 0.04369776644129782 4.2230264741529664E-5 0.0 2.1115132370764832E-5 0.0010451990523528592 76-77 0.052713927963614404 4.2230264741529664E-5 0.0 2.1115132370764832E-5 0.0010768717509090065 78-79 0.06524575902566333 4.2230264741529664E-5 0.0 2.1115132370764832E-5 0.001171889846577448 80-81 0.079012825331402 4.2230264741529664E-5 0.0 2.1115132370764832E-5 0.0012035625451335954 82-83 0.09579935556616004 4.2230264741529664E-5 0.0 2.1115132370764832E-5 0.0012246776775043603 84-85 0.11561590729612284 4.2230264741529664E-5 0.0 2.1115132370764832E-5 0.0012246776775043603 86-87 0.14077458751588912 4.2230264741529664E-5 0.0 2.1115132370764832E-5 0.0012246776775043603 88-89 0.17189829263039652 4.2230264741529664E-5 0.0 2.1115132370764832E-5 0.00126690794224589 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCTCG 1715 0.0 63.97627 2 ATATCTC 2145 0.0 51.819313 1 ATATGGA 3760 0.0 49.39603 1 GGATCCG 5840 0.0 39.28223 5 TCTCGAG 3245 0.0 39.2263 4 TATGGAT 4905 0.0 38.34663 2 ATCTCGA 3100 0.0 38.30446 3 ATGGATC 5915 0.0 36.295662 3 TCGAGGG 3795 0.0 33.66684 6 GATCCGG 6770 0.0 33.60537 6 ATCCGGC 7190 0.0 31.972626 7 CTCGAGG 4030 0.0 31.703638 5 TCCGGCG 8090 0.0 28.47443 8 TGGATCC 8065 0.0 27.855698 4 CGAGGGC 4875 0.0 26.013483 7 AGGGCGC 5190 0.0 24.526144 9 CCGGCGC 10270 0.0 22.152714 9 GGGCGCG 4855 0.0 20.935648 8 CGTCGCG 195 1.033566E-5 19.487606 1 GAGGGCG 7345 0.0 17.588911 8 >>END_MODULE