FastQCFastQC Report
Tue 31 May 2016
SRR1051262_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051262_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4442880
Sequences flagged as poor quality0
Sequence length101
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT103730223.34751332469029No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT94976221.377169763756843No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA297950.6706235594929415No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTA262310.5904053226735811No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAA260380.5860612935753384No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223880.5039073753961394No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTAAA164940.37124567847882456No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT160430.361094605301066No Hit
CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152650.3435834413713627No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146340.3293809420916163No Hit
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137520.30952895419187554No Hit
ATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT123900.27887316335350043No Hit
GGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111090.25004051426101986No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA100940.2271949726303659No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA95890.21582847162201096No Hit
ATATCTCGAGGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT86730.1952112143474503No Hit
ATATGGATCTGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT70780.15931107749927975No Hit
ATATGGATTCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT70100.15778053874963988No Hit
TGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67770.15253619273984442No Hit
ATATGGACCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT65150.14663911696917314No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTC65120.14657159320080668No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62800.14134975511380005No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61470.1383562013828868No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTAAA60050.1351600763468741No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTAAAA58340.1313112215499856No Hit
TATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT58000.13054595217516565No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT56850.12795754105445117No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC56600.1273948429847306No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTG56200.12649452607317774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGGA1293750.092.176981
TATGGAT1297600.091.349242
ATATCTC1118950.090.949961
TATCTCG1117000.090.4912642
ATGGATC1303750.090.160533
TGGATCC1304100.089.264824
GGATCCG1304450.088.960485
ATCTCGA1134550.088.936583
TCTCGAG1139200.088.5266954
CTCGAGG1149000.087.796455
TCGAGGG1151500.087.654346
CGAGGGC1155000.087.253017
GATCCGG1336650.087.082956
AGGGCGC1171350.086.700139
GAGGGCG1169250.086.603988
ATCCGGC1352900.086.001887
TCCGGCG1368150.085.3314068
CCGGCGC1399500.083.837379
TATCGAT3750.063.3325462
ATTCGGC13050.061.5112657
Produced by FastQC (version 0.11.5)