##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1051262_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4442880
Sequences flagged as poor quality	0
Sequence length	101
%GC	34
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.930671771463555	33.0	31.0	34.0	30.0	34.0
2	32.19964189894843	34.0	31.0	34.0	30.0	34.0
3	32.21106309420916	34.0	31.0	34.0	30.0	34.0
4	35.63497506122155	37.0	35.0	37.0	33.0	37.0
5	35.62110635443676	37.0	35.0	37.0	33.0	37.0
6	35.57610783995967	37.0	35.0	37.0	33.0	37.0
7	35.51974079876116	37.0	35.0	37.0	33.0	37.0
8	35.510534158023624	37.0	35.0	37.0	33.0	37.0
9	37.12195197709594	39.0	37.0	39.0	33.0	39.0
10-11	37.14409932296168	39.0	37.0	39.0	33.5	39.0
12-13	37.203822520527225	39.0	37.5	39.0	34.0	39.0
14-15	38.74721678280755	41.0	39.0	41.0	35.0	41.0
16-17	38.70083830758427	41.0	39.0	41.0	34.5	41.0
18-19	38.71868810321233	41.0	39.0	41.0	34.5	41.0
20-21	37.89083376098387	40.0	38.0	41.0	32.5	41.0
22-23	37.78531718164794	40.0	38.0	41.0	32.0	41.0
24-25	38.11677920628061	40.0	38.0	41.0	32.5	41.0
26-27	38.510508161372805	40.5	38.0	41.0	34.0	41.0
28-29	38.63583857767934	41.0	38.0	41.0	34.0	41.0
30-31	38.16094245174301	40.0	38.0	40.0	34.0	41.0
32-33	35.99924665982426	36.0	35.0	39.0	33.0	41.0
34-35	35.69533275713051	35.0	35.0	39.0	33.0	41.0
36-37	35.549142898300204	35.0	35.0	39.0	33.0	41.0
38-39	35.4668436914794	35.0	35.0	38.5	33.0	41.0
40-41	35.40401473818784	35.0	35.0	38.0	33.0	41.0
42-43	35.336821386127916	35.0	35.0	38.0	33.0	41.0
44-45	35.21512442379718	35.0	35.0	38.0	33.0	41.0
46-47	34.75880509939499	35.0	35.0	38.0	30.5	41.0
48-49	33.67561761740133	35.0	34.0	38.0	23.5	41.0
50-51	32.619506266205704	35.0	32.0	36.5	21.0	40.0
52-53	30.703460142970325	35.0	30.5	37.0	9.5	40.0
54-55	29.108082032375396	35.0	25.5	37.0	2.0	41.0
56-57	27.724492783959953	35.0	20.5	37.0	2.0	41.0
58-59	26.856696669727746	35.0	12.5	36.0	2.0	40.0
60-61	26.19035996920916	35.0	7.0	36.0	2.0	40.0
62-63	25.577500630221838	35.0	3.5	35.0	2.0	40.0
64-65	24.988032199834343	35.0	2.0	35.0	2.0	39.5
66-67	24.286642448141748	34.0	2.0	35.0	2.0	39.0
68-69	23.56201551696197	33.0	2.0	35.0	2.0	39.0
70-71	23.0308819954624	33.0	2.0	35.0	2.0	38.5
72-73	22.411041599142898	33.0	2.0	35.0	2.0	37.0
74-75	21.762208297320658	33.0	2.0	35.0	2.0	37.0
76-77	21.14772951328868	31.5	2.0	35.0	2.0	36.5
78-79	20.661616676570155	31.0	2.0	35.0	2.0	36.0
80-81	20.230674359874676	30.5	2.0	35.0	2.0	35.5
82-83	19.845172838339096	29.5	2.0	35.0	2.0	35.0
84-85	19.462086079299915	29.0	2.0	35.0	2.0	35.0
86-87	19.137851911372803	27.5	2.0	35.0	2.0	35.0
88-89	18.82319610252809	26.5	2.0	35.0	2.0	35.0
90-91	18.522454691551424	24.5	2.0	35.0	2.0	35.0
92-93	18.22121934420916	22.5	2.0	35.0	2.0	35.0
94-95	17.913781038425526	17.0	2.0	35.0	2.0	35.0
96-97	17.600968628457217	2.0	2.0	35.0	2.0	35.0
98-99	17.20401755167819	2.0	2.0	35.0	2.0	35.0
100-101	16.777759358794295	2.0	2.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.511566135863962
1101	2	0.4406966603875162
1101	3	0.4599633623695709
1101	4	0.3464582090527273
1101	5	0.3498546885371354
1101	6	0.37789105845201476
1101	7	0.526141307759211
1101	8	0.46964611479101137
1101	9	0.556915822340919
1101	10-11	0.46946951433287154
1101	12-13	0.4411490361570074
1101	14-15	0.6450055964927444
1101	16-17	0.6094931110351283
1101	18-19	0.6252830998052019
1101	20-21	0.6428021000282698
1101	22-23	0.6485790021642757
1101	24-25	0.6161919007638659
1101	26-27	0.48583000861704306
1101	28-29	0.4984008584058017
1101	30-31	0.43622684576125437
1101	32-33	0.4739279954811124
1101	34-35	0.48908474384896294
1101	36-37	0.5538540638234721
1101	38-39	0.5696284067291444
1101	40-41	0.5388360626638473
1101	42-43	0.5153000310858218
1101	44-45	0.4708895861051019
1101	46-47	0.46056019589291
1101	48-49	0.39864920652458835
1101	50-51	0.21860661473291998
1101	52-53	-0.19669670109406567
1101	54-55	-0.4952931454513312
1101	56-57	-0.42314522553823863
1101	58-59	-0.5261034415080168
1101	60-61	-0.690789417036985
1101	62-63	-0.7125498052846417
1101	64-65	-0.7242874380892061
1101	66-67	-0.6440092893240354
1101	68-69	-0.6010627809203939
1101	70-71	-0.6469617329938657
1101	72-73	-0.6926450679342935
1101	74-75	-0.7723202790107067
1101	76-77	-0.7010898120543771
1101	78-79	-0.8021637696986907
1101	80-81	-0.806239163355329
1101	82-83	-0.7861082781251412
1101	84-85	-0.6494459515968174
1101	86-87	-0.5782778515033691
1101	88-89	-0.6106890186119713
1101	90-91	-0.5364427650633061
1101	92-93	-0.5110378847631019
1101	94-95	-0.47401729427753736
1101	96-97	-0.43596449080970956
1101	98-99	-0.36814569190971724
1101	100-101	-0.35551150783471286
1102	1	0.47043513672833726
1102	2	0.3079977629230939
1102	3	0.31950035569786195
1102	4	0.30188101435609127
1102	5	0.3188479810949687
1102	6	0.3753694577928428
1102	7	0.4354965954828316
1102	8	0.28479547860856513
1102	9	0.42687107728330886
1102	10-11	0.4794717307227927
1102	12-13	0.4295369994751326
1102	14-15	0.521372225394245
1102	16-17	0.5129507302666525
1102	18-19	0.5502065932865463
1102	20-21	0.6169240241352938
1102	22-23	0.682718361232503
1102	24-25	0.6701803744571748
1102	26-27	0.555197827306884
1102	28-29	0.5975108672437841
1102	30-31	0.4811117841297943
1102	32-33	0.47961388692728946
1102	34-35	0.4230298345722687
1102	36-37	0.40398515876085384
1102	38-39	0.4861202062063441
1102	40-41	0.42300456992732904
1102	42-43	0.42468340587130626
1102	44-45	0.3986097356134053
1102	46-47	0.3655946773326093
1102	48-49	0.49868393016649293
1102	50-51	0.28340886203358906
1102	52-53	0.05823326769901982
1102	54-55	-0.21826928529744194
1102	56-57	-0.07301600407082987
1102	58-59	-0.08214540315877272
1102	60-61	-0.2810719051346062
1102	62-63	-0.4355067607982086
1102	64-65	-0.349613631693412
1102	66-67	-0.2193284176982715
1102	68-69	-0.16148564977185842
1102	70-71	-0.284504149852868
1102	72-73	-0.36149687004454734
1102	74-75	-0.40240333278235596
1102	76-77	-0.3868977164550138
1102	78-79	-0.4202535731180319
1102	80-81	-0.34004666344615586
1102	82-83	-0.32668333628664215
1102	84-85	-0.32477574825848166
1102	86-87	-0.37210684224321255
1102	88-89	-0.4217999605279239
1102	90-91	-0.41664880839184093
1102	92-93	-0.3407878745414479
1102	94-95	-0.29177460323845494
1102	96-97	-0.22686365927680185
1102	98-99	-0.09819215756214916
1102	100-101	-0.11291949288148473
1103	1	0.34767782248240664
1103	2	0.15340411281961508
1103	3	0.24943791043514096
1103	4	0.23885538676670137
1103	5	0.2673675257488739
1103	6	0.24843403571345135
1103	7	0.3262607414296994
1103	8	0.25407787875298027
1103	9	0.32164206469182943
1103	10-11	0.32111923632569983
1103	12-13	0.33600635116494715
1103	14-15	0.294427286624817
1103	16-17	0.3333123454776441
1103	18-19	0.3965884322867339
1103	20-21	0.4348957768740931
1103	22-23	0.4972900395288846
1103	24-25	0.545066650862374
1103	26-27	0.45465737351455005
1103	28-29	0.49131561552166403
1103	30-31	0.43077119643417916
1103	32-33	0.3334959289445223
1103	34-35	0.3796746222153047
1103	36-37	0.31711349793727805
1103	38-39	0.31500526723318245
1103	40-41	0.40137637378500557
1103	42-43	0.3358978944161777
1103	44-45	0.37097805160701114
1103	46-47	0.3985249714652923
1103	48-49	0.427935468882481
1103	50-51	0.3874970067858001
1103	52-53	0.22193123922533786
1103	54-55	-0.1522535471776827
1103	56-57	-0.17674380420649172
1103	58-59	-0.2577470755992941
1103	60-61	-0.3167943788180061
1103	62-63	-0.39710606524564085
1103	64-65	-0.4282672234948244
1103	66-67	-0.40645537210265914
1103	68-69	-0.3761580804201863
1103	70-71	-0.52603973645693
1103	72-73	-0.4989003248697479
1103	74-75	-0.5182855478251582
1103	76-77	-0.4904306074580411
1103	78-79	-0.4363212941000292
1103	80-81	-0.5023287577739772
1103	82-83	-0.4770085707294811
1103	84-85	-0.45084446063329153
1103	86-87	-0.3887739955265559
1103	88-89	-0.30644766111798916
1103	90-91	-0.3486181792909626
1103	92-93	-0.2990144146662139
1103	94-95	-0.3397380613618708
1103	96-97	-0.2811488284545547
1103	98-99	-0.124931389416016
1103	100-101	-0.03734731962642357
1104	1	0.31405530726073394
1104	2	0.26150857482503653
1104	3	0.2682811444115245
1104	4	0.28314025593238057
1104	5	0.23478659247159328
1104	6	0.27023936175802277
1104	7	0.38266840429745486
1104	8	0.32015644191211834
1104	9	0.3745231885865863
1104	10-11	0.40997027534339026
1104	12-13	0.38710637409374016
1104	14-15	0.40780694229479053
1104	16-17	0.35662224809099285
1104	18-19	0.37549991352148027
1104	20-21	0.3796791515616391
1104	22-23	0.5225330005848576
1104	24-25	0.6004098640830193
1104	26-27	0.4980898212809919
1104	28-29	0.39520186820220005
1104	30-31	0.3700384965238612
1104	32-33	0.32496626283430174
1104	34-35	0.28561061125898135
1104	36-37	0.3466574521312751
1104	38-39	0.3259034139004271
1104	40-41	0.2978523470751142
1104	42-43	0.1726568797987511
1104	44-45	0.2778370796473695
1104	46-47	0.32956055491039393
1104	48-49	0.32859481512897304
1104	50-51	0.17277710228766097
1104	52-53	0.24842391796037688
1104	54-55	0.1516291158128915
1104	56-57	0.2034247482431013
1104	58-59	0.09262777797007615
1104	60-61	0.09105399740874276
1104	62-63	0.047594429453148734
1104	64-65	0.004041062302405862
1104	66-67	0.008187005749565657
1104	68-69	0.029419368518421862
1104	70-71	-0.0129335925478955
1104	72-73	0.023540916961270142
1104	74-75	0.1326439024461834
1104	76-77	0.2234583007092965
1104	78-79	0.21024496080242372
1104	80-81	0.14749339921171867
1104	82-83	0.06520017226324626
1104	84-85	0.021377265548018443
1104	86-87	-0.0926541345569909
1104	88-89	-0.10627787346153639
1104	90-91	-0.06755773704791679
1104	92-93	-0.0353653009076389
1104	94-95	-0.02373974635870013
1104	96-97	0.08368452168351936
1104	98-99	0.140451972186451
1104	100-101	0.2746267410815477
1105	1	0.17638557293615165
1105	2	0.06544167423965774
1105	3	0.09109843427737019
1105	4	0.07715954742337061
1105	5	0.04922448878632224
1105	6	0.03743187965750394
1105	7	0.15117761159997656
1105	8	0.02858226465748004
1105	9	0.16052242424875374
1105	10-11	0.12533447984456814
1105	12-13	0.09037741842904268
1105	14-15	0.10068171828072536
1105	16-17	0.11639466588447789
1105	18-19	0.082026245454351
1105	20-21	0.06439544989085988
1105	22-23	-0.21835977815418772
1105	24-25	-0.4816011112145233
1105	26-27	-0.3174952125391002
1105	28-29	-0.5873521950943754
1105	30-31	-0.28622845526472673
1105	32-33	-0.1569428254887697
1105	34-35	0.10706086189500041
1105	36-37	0.16721188151845467
1105	38-39	0.21308994572372342
1105	40-41	0.17523723569575367
1105	42-43	0.13020184648574684
1105	44-45	0.08086035732091545
1105	46-47	-0.11338746393337829
1105	48-49	-0.2913659414417964
1105	50-51	-0.44737760809879745
1105	52-53	-0.265956986951295
1105	54-55	-0.1395411868178904
1105	56-57	0.0753471568224846
1105	58-59	0.3098683081583289
1105	60-61	0.42603564959050644
1105	62-63	0.47651024469117687
1105	64-65	0.4645528347127801
1105	66-67	0.47247068639487466
1105	68-69	0.3632049680474516
1105	70-71	0.41688605835502557
1105	72-73	0.1864904446920157
1105	74-75	-0.012474724516138735
1105	76-77	-0.010520522927791376
1105	78-79	0.1224274916016519
1105	80-81	0.07150202955049068
1105	82-83	0.2590607956314557
1105	84-85	0.3958006913893115
1105	86-87	0.40472266544004043
1105	88-89	0.33637687712068143
1105	90-91	0.40151177440663943
1105	92-93	0.3721826588174473
1105	94-95	0.39688103695152677
1105	96-97	0.3237971192819131
1105	98-99	0.2977866841312711
1105	100-101	0.04461539969339512
1106	1	-0.04636972688073371
1106	2	-0.16508538734315437
1106	3	-0.20213594897541753
1106	4	-0.18580053205933922
1106	5	-0.10414079895458883
1106	6	-0.10703229844333606
1106	7	-0.02164195380456846
1106	8	-0.048544514454576415
1106	9	-0.1062054521714515
1106	10-11	-0.1518894771193544
1106	12-13	-0.13896316126424324
1106	14-15	-0.1645459336054742
1106	16-17	-0.19104388974526643
1106	18-19	-0.12411344494466903
1106	20-21	-0.12408068551827256
1106	22-23	-0.004185144214417846
1106	24-25	0.011918697803594114
1106	26-27	0.10506663901716706
1106	28-29	0.04012728854721814
1106	30-31	-0.055405695195020144
1106	32-33	-0.10549275463840502
1106	34-35	-0.15745310923900746
1106	36-37	-0.1788185666886477
1106	38-39	-0.25635657213778273
1106	40-41	-0.160081858536401
1106	42-43	-0.22035353197620822
1106	44-45	-0.13995790043480838
1106	46-47	-0.03639891865778111
1106	48-49	0.0782834364943028
1106	50-51	0.040347478515492696
1106	52-53	0.2080491068783239
1106	54-55	0.21672884964270978
1106	56-57	0.4219793096266038
1106	58-59	0.4186953332107386
1106	60-61	0.292639276105529
1106	62-63	0.2639204252723566
1106	64-65	0.3294067247353283
1106	66-67	0.5011497633158015
1106	68-69	0.5972653106886732
1106	70-71	0.572383104511303
1106	72-73	0.5980200331549206
1106	74-75	0.6311832950706702
1106	76-77	0.6250059802284831
1106	78-79	0.5173551171952688
1106	80-81	0.44511613121669313
1106	82-83	0.4095173093280309
1106	84-85	0.41540566278158764
1106	86-87	0.3525537832936365
1106	88-89	0.22423767301810926
1106	90-91	0.171369108059519
1106	92-93	0.18721195294303428
1106	94-95	0.18109811130834785
1106	96-97	0.11309387412568128
1106	98-99	0.219170372026678
1106	100-101	0.29617388672975764
1107	1	-0.3052425617911645
1107	2	-0.3848397206948455
1107	3	-0.41846906158346897
1107	4	-0.36157865213473883
1107	5	-0.31214806757607505
1107	6	-0.40170247657938063
1107	7	-0.15902584566423883
1107	8	-0.2802878093424539
1107	9	-0.26716215287583367
1107	10-11	-0.3023081212400598
1107	12-13	-0.3196611645760541
1107	14-15	-0.3363073547852693
1107	16-17	-0.3538331327167654
1107	18-19	-0.4082332252549037
1107	20-21	-0.5015455498855843
1107	22-23	-0.7881102141988805
1107	24-25	-0.4472592862156901
1107	26-27	-0.44139167617971964
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2308	22-23	-1.3409286965411056
2308	24-25	-1.939961087006175
2308	26-27	-1.1378714734721953
2308	28-29	-1.2995720836759048
2308	30-31	-1.662057973128178
2308	32-33	-1.1711385475053717
2308	34-35	-1.0520232425299483
2308	36-37	-1.4524920297489388
2308	38-39	-1.2113059130034642
2308	40-41	-1.2853457624442228
2308	42-43	-0.9388501368295579
2308	44-45	-1.1451508863731945
2308	46-47	-1.2967402940838753
2308	48-49	-0.9480611166813873
2308	50-51	-0.5290153342114934
2308	52-53	-0.27669230574351644
2308	54-55	0.3345854291122201
2308	56-57	0.7561392490231249
2308	58-59	0.42104994062885126
2308	60-61	0.5629572851817386
2308	62-63	0.606700803826989
2308	64-65	0.6588173429105169
2308	66-67	0.8384257899626029
2308	68-69	0.7143435634540047
2308	70-71	0.6754725336704084
2308	72-73	0.9881326690657986
2308	74-75	1.234834414396559
2308	76-77	1.112210345915578
2308	78-79	1.2881191759556039
2308	80-81	1.038751940270778
2308	82-83	0.7988721036983577
2308	84-85	0.9400956754691236
2308	86-87	1.2314646121490327
2308	88-89	1.2460854498661824
2308	90-91	1.271900635693715
2308	92-93	1.2609778103579252
2308	94-95	0.9785220012899138
2308	96-97	0.9998986154596992
2308	98-99	1.0570497527548888
2308	100-101	1.1710038118072355
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	275.0
11	1824.0
12	6095.0
13	11895.0
14	15592.0
15	20694.0
16	29312.0
17	45282.0
18	85100.0
19	339110.0
20	393001.0
21	237325.0
22	187894.0
23	160301.0
24	136675.0
25	119881.0
26	107811.0
27	104383.0
28	101803.0
29	100103.0
30	99877.0
31	103713.0
32	113119.0
33	129583.0
34	162540.0
35	292172.0
36	456164.0
37	422122.0
38	386219.0
39	72901.0
40	111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.141523524598375	68.05613802868582	10.52701718605261	10.275321260663198
2	11.062016918964046	14.226056321067313	65.69717516820262	9.01475159176601
3	11.225325143475938	65.85467362304853	12.733969263500311	10.186031969975225
4	11.564323141745895	12.251264945260731	65.16203453615674	11.022377376836646
5	39.92524547538404	12.031625044341327	11.582607215976923	36.460522264297715
6	41.0752259795448	11.457320476807837	35.41144482857966	12.056008715067703
7	12.556472378277153	40.0269869994238	11.262064246614809	36.15447637568424
8	36.15409374099683	11.553564354652837	39.384678406799196	12.90766349755114
9	11.993166594641313	35.42717786660904	11.67233866320945	40.90731687554019
10-11	50.96651271247479	11.33662174085278	11.303861459233651	26.393004087438776
12-13	38.5542823573898	11.326200572601556	11.472299499423798	38.64721757058484
14-15	38.052119346009796	11.666891745894555	11.944673274992798	38.33631563310285
16-17	37.724347720397574	11.684875576202822	12.177585260011524	38.41319144338807
18-19	36.906128457216944	11.859075644626909	13.180628331172572	38.05416756698358
20-21	22.98962609838663	23.3986400713051	28.55708459377701	25.054649236531258
22-23	24.393794421182303	25.578047920238422	27.004173680759674	23.023983977819597
24-25	9.73200711568594	11.150879033901695	42.156770396009506	36.96034345440285
26-27	9.630547873133594	10.944790661426051	69.43516707978246	9.989494385657895
28-29	9.757344019007693	11.031789851205302	69.42381133839602	9.787054791390988
30-31	9.59636542062806	10.959625288101412	69.60063067199654	9.843378619273984
32-33	9.479009111207144	11.013846874099684	69.74949807332182	9.757645941371361
34-35	9.445112179487179	10.993747299049266	69.8060829912129	9.755057530250648
36-37	9.43850610414866	10.95393078363584	69.91650686041486	9.691056251800633
38-39	9.282019770959378	11.009525352924229	70.06983083045232	9.638624045664073
40-41	9.337895689282627	11.070578093488908	70.06634210242005	9.525184114808413
42-43	9.31797617761452	11.006644338807261	70.04405925885912	9.6313202247191
44-45	9.279363836070296	11.235437373955632	69.93301642178047	9.552182368193604
46-47	9.360459881878421	11.540509759435322	69.42779233290118	9.671238025785076
48-49	9.643478225638361	12.871817646108157	67.5951931954923	9.889510932761189
50-51	9.98931766425681	14.51144268258876	65.27118737352642	10.228052279628006
52-53	10.498245833601572	15.699552916039963	63.08619838174605	10.716002868612419
54-55	11.68503266667387	17.30614517041055	59.01842857811729	11.99039358479829
56-57	12.627981470125398	18.508897214070288	55.81169824659885	13.051423069205455
58-59	13.090900496974935	19.26765971622011	53.766374513828865	13.875065272976087
60-61	13.470102726159608	19.544754753673292	52.43478329371939	14.550359226447709
62-63	13.876708351339673	19.63307584269663	51.74664857029675	14.743567235666955
64-65	14.136922446701238	19.59451977095938	51.32394978032268	14.94460800201671
66-67	14.411029472921019	19.498873343455852	50.9282184555582	15.161878728064918
68-69	14.523147147796026	19.83752655934889	50.5653990204552	15.073927272399883
70-71	14.92123352420052	20.23843768006338	49.836502448862	15.0038263468741
72-73	15.366490440825695	20.63871429299721	48.84708190872201	15.14771335745508
74-75	15.708421768283342	21.242090935906717	47.59052720866604	15.4589600871439
76-77	16.116684824692516	21.90506203742054	46.340546271451984	15.637706866434964
78-79	16.434965572332537	22.58468940596602	45.25923219920442	15.72111282249702
80-81	16.49526860778124	23.26339398205039	44.195017337502904	16.04632007266547
82-83	16.77381563310285	23.743922860847018	43.18063958513397	16.301621920916162
84-85	16.966044547680784	23.965445836934602	42.387437878133106	16.681071737251514
86-87	17.101744814174587	24.281457072889655	41.86520905358686	16.75158905934889
88-89	17.282145815326995	24.72140818568136	41.13329642033996	16.863149578651683
90-91	17.59626638576779	24.85170655070585	40.54511938202247	17.00690768150389
92-93	17.90187896139441	24.91766601843849	39.984604580812444	17.195850439354654
94-95	18.17536147723999	25.000799031259003	39.277698249783924	17.546141241717084
96-97	18.382985811005472	25.22343614952463	38.71704840103716	17.67652963843273
98-99	18.74231354436762	25.241048599106886	38.192962672860844	17.82367518366465
100-101	18.92845575882785	25.500107001548677	37.51778246410664	18.05365477551683
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	26455.0
1	14786.5
2	2438.5
3	1543.0
4	1321.0
5	1268.5
6	1262.5
7	1350.5
8	1430.5
9	1484.5
10	2155.5
11	2824.5
12	3462.0
13	6773.0
14	15122.5
15	29435.5
16	340520.5
17	389244.0
18	121143.0
19	102836.5
20	96312.5
21	92193.5
22	89293.5
23	87075.5
24	85227.5
25	82957.0
26	81215.0
27	79650.5
28	77968.5
29	76583.5
30	75329.0
31	74003.0
32	72801.0
33	73010.0
34	73326.0
35	73032.5
36	73493.5
37	74261.5
38	74385.5
39	74501.0
40	74799.5
41	75082.0
42	75185.0
43	75805.0
44	77702.5
45	80034.0
46	83114.5
47	86531.0
48	89171.5
49	91615.5
50	90508.5
51	87799.0
52	87895.5
53	87304.0
54	84475.0
55	81638.0
56	76738.5
57	70146.0
58	62898.0
59	54665.5
60	46765.0
61	39073.5
62	32160.5
63	25137.5
64	18312.0
65	12861.5
66	9130.5
67	6925.5
68	5266.5
69	3794.5
70	2822.5
71	1938.0
72	1393.0
73	1051.5
74	802.0
75	701.0
76	573.5
77	362.0
78	203.0
79	114.0
80	60.5
81	29.5
82	13.5
83	7.5
84	5.5
85	3.0
86	3.5
87	2.0
88	0.0
89	1.0
90	1.5
91	1.0
92	0.5
93	0.5
94	2.0
95	1.5
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013842372515125325
2	0.0038938706424661482
3	0.003353680495534428
4	0.0
5	0.00162057044079516
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	8.77808988764045E-4
24-25	4.389044943820225E-4
26-27	3.9388864880437915E-4
28-29	0.0010578723710746184
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.004006410256410256
50-51	0.011242707433016422
52-53	0.007067487755690003
54-55	0.00972342264477096
56-57	1.1253961394410832E-5
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.006988710025929127
68-69	0.0
70-71	0.0
72-73	3.3761884183232494E-5
74-75	0.002554649236531259
76-77	0.009003169115528665
78-79	0.0019581892826274847
80-81	0.0012266817919907808
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.030509489340247765
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4442880.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.062467146530285
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.08949358319857	24.14785677483548
2	8.652783152919213	5.029424522155307
3	2.7487455363001834	2.3965598052868757
4	1.5231553137069425	1.770666050546842
5	1.006243706016063	1.4621962323747357
6	0.6972253048789321	1.2157852510064115
7	0.5115826723521841	1.0407498225578662
8	0.3865232208857961	0.8986654726691615
9	0.29028755585537963	0.759282529958419
>10	1.0376937164191484	4.426803106722824
>50	0.01908558247917384	0.3949242059895924
>100	0.026068753556823587	1.684915608701568
>500	0.004444760572660038	0.9022700495242987
>1k	0.004444760572659855	2.6343158503105335
>5k	0.0011495070446534109	2.2090159671718217
>10k+	0.0010728732416765167	49.02656875018833
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	1037302	23.34751332469029	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	949762	21.377169763756843	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29795	0.6706235594929415	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTA	26231	0.5904053226735811	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAA	26038	0.5860612935753384	No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22388	0.5039073753961394	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTAAA	16494	0.37124567847882456	No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16043	0.361094605301066	No Hit
CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15265	0.3435834413713627	No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14634	0.3293809420916163	No Hit
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13752	0.30952895419187554	No Hit
ATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12390	0.27887316335350043	No Hit
GGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11109	0.25004051426101986	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA	10094	0.2271949726303659	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA	9589	0.21582847162201096	No Hit
ATATCTCGAGGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	8673	0.1952112143474503	No Hit
ATATGGATCTGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	7078	0.15931107749927975	No Hit
ATATGGATTCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	7010	0.15778053874963988	No Hit
TGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6777	0.15253619273984442	No Hit
ATATGGACCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	6515	0.14663911696917314	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTC	6512	0.14657159320080668	No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6280	0.14134975511380005	No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6147	0.1383562013828868	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTAAA	6005	0.1351600763468741	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTAAAA	5834	0.1313112215499856	No Hit
TATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5800	0.13054595217516565	No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT	5685	0.12795754105445117	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC	5660	0.1273948429847306	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTG	5620	0.12649452607317774	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.007832757130509939	2.2507922788821664E-5	0.0	2.2507922788821664E-5	0.0
2	0.007832757130509939	2.2507922788821664E-5	0.0	4.501584557764333E-5	0.0
3	0.007832757130509939	2.2507922788821664E-5	0.0	4.501584557764333E-5	0.0
4	0.007855265053298761	4.501584557764333E-5	0.0	4.501584557764333E-5	0.0
5	0.007877772976087582	4.501584557764333E-5	0.0	4.501584557764333E-5	0.0
6	0.007877772976087582	4.501584557764333E-5	0.0	4.501584557764333E-5	4.501584557764333E-5
7	0.007900280898876405	4.501584557764333E-5	0.0	6.7523768366465E-5	4.501584557764333E-5
8	0.007922788821665225	4.501584557764333E-5	0.0	6.7523768366465E-5	4.501584557764333E-5
9	0.007922788821665225	4.501584557764333E-5	0.0	9.003169115528665E-5	6.7523768366465E-5
10-11	0.007922788821665225	4.501584557764333E-5	0.0	1.1253961394410832E-4	1.1253961394410832E-4
12-13	0.0080015665514261	4.501584557764333E-5	0.0	1.1253961394410832E-4	1.1253961394410832E-4
14-15	0.008035328435609335	4.501584557764333E-5	0.0	1.1253961394410832E-4	1.1253961394410832E-4
16-17	0.008136614088159033	4.501584557764333E-5	0.0	1.2379357533851915E-4	1.1253961394410832E-4
18-19	0.00827166162489196	4.501584557764333E-5	0.0	1.5755545952175166E-4	1.1253961394410832E-4
20-21	0.008462978968596947	4.501584557764333E-5	0.0	1.5755545952175166E-4	1.1253961394410832E-4
22-23	0.008688058196485163	4.501584557764333E-5	0.0	2.02571305099395E-4	1.3504753673293E-4
24-25	0.008980661192739844	4.501584557764333E-5	0.0	2.02571305099395E-4	1.3504753673293E-4
26-27	0.009262010227600115	4.501584557764333E-5	0.0	2.2507922788821664E-4	1.3504753673293E-4
28-29	0.009565867185249207	4.501584557764333E-5	0.0	2.2507922788821664E-4	1.3504753673293E-4
30-31	0.009723422644770958	4.501584557764333E-5	0.0	2.2507922788821664E-4	2.2507922788821664E-4
32-33	0.009779692451743014	4.501584557764333E-5	0.0	2.2507922788821664E-4	2.2507922788821664E-4
34-35	0.009925993949870354	4.501584557764333E-5	0.0	2.2507922788821664E-4	2.2507922788821664E-4
36-37	0.010241104868913858	4.501584557764333E-5	0.0	2.2507922788821664E-4	2.475871506770383E-4
38-39	0.010353644482857966	4.501584557764333E-5	0.0	2.2507922788821664E-4	2.475871506770383E-4
40-41	0.01054496182656295	4.501584557764333E-5	0.0	2.2507922788821664E-4	2.7009507346586E-4
42-43	0.01082631086142322	4.501584557764333E-5	0.0	2.2507922788821664E-4	2.8134903486027083E-4
44-45	0.011141421780466725	4.501584557764333E-5	0.0	2.2507922788821664E-4	3.151109190435033E-4
46-47	0.011389008931143762	4.501584557764333E-5	0.0	2.2507922788821664E-4	3.2636488043791414E-4
48-49	0.01177164361855373	4.501584557764333E-5	0.0	2.2507922788821664E-4	3.601267646211466E-4
50-51	0.0121542783059637	4.501584557764333E-5	0.0	2.2507922788821664E-4	3.601267646211466E-4
52-53	0.013268420484010371	4.501584557764333E-5	0.0	2.2507922788821664E-4	3.826346874099683E-4
54-55	0.014528864160184386	4.501584557764333E-5	0.0	2.2507922788821664E-4	3.9388864880437915E-4
56-57	0.016048148948429847	4.501584557764333E-5	0.0	2.2507922788821664E-4	4.163965715932008E-4
58-59	0.017803766925957934	4.501584557764333E-5	0.0	2.475871506770383E-4	4.2765053298761163E-4
60-61	0.019953273552290405	4.501584557764333E-5	0.0	2.588411120714491E-4	4.3890449438202246E-4
62-63	0.022147796024200517	4.501584557764333E-5	0.0	2.7009507346586E-4	4.501584557764333E-4
64-65	0.025467714635551714	4.501584557764333E-5	0.0	2.7009507346586E-4	4.501584557764333E-4
66-67	0.02936158527801786	4.501584557764333E-5	0.0	2.7009507346586E-4	4.501584557764333E-4
68-69	0.034572169403630074	4.501584557764333E-5	0.0	2.7009507346586E-4	4.501584557764333E-4
70-71	0.040345451598962835	5.626980697205416E-5	0.0	2.7009507346586E-4	4.501584557764333E-4
72-73	0.04755924085278018	6.7523768366465E-5	0.0	2.7009507346586E-4	4.8392033995966577E-4
74-75	0.05529071233074042	6.7523768366465E-5	0.0	2.7009507346586E-4	5.289361855373091E-4
76-77	0.0656443568135984	6.7523768366465E-5	0.0	2.7009507346586E-4	5.852059925093633E-4
78-79	0.07723593704984154	6.7523768366465E-5	0.0	2.7009507346586E-4	6.07713915298185E-4
80-81	0.09062815110919042	6.7523768366465E-5	0.0	2.7009507346586E-4	6.639837222702391E-4
82-83	0.10657501440507058	6.7523768366465E-5	0.0	2.7009507346586E-4	6.864916450590608E-4
84-85	0.12730481129357535	6.7523768366465E-5	0.0	2.8134903486027083E-4	7.42761452031115E-4
86-87	0.1506117653414002	6.7523768366465E-5	0.0	2.9260299625468166E-4	7.540154134255258E-4
88-89	0.1790055099394987	6.7523768366465E-5	0.0	2.9260299625468166E-4	7.652693748199367E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATGGA	129375	0.0	92.17698	1
TATGGAT	129760	0.0	91.34924	2
ATATCTC	111895	0.0	90.94996	1
TATCTCG	111700	0.0	90.491264	2
ATGGATC	130375	0.0	90.16053	3
TGGATCC	130410	0.0	89.26482	4
GGATCCG	130445	0.0	88.96048	5
ATCTCGA	113455	0.0	88.93658	3
TCTCGAG	113920	0.0	88.526695	4
CTCGAGG	114900	0.0	87.79645	5
TCGAGGG	115150	0.0	87.65434	6
CGAGGGC	115500	0.0	87.25301	7
GATCCGG	133665	0.0	87.08295	6
AGGGCGC	117135	0.0	86.70013	9
GAGGGCG	116925	0.0	86.60398	8
ATCCGGC	135290	0.0	86.00188	7
TCCGGCG	136815	0.0	85.331406	8
CCGGCGC	139950	0.0	83.83737	9
TATCGAT	375	0.0	63.332546	2
ATTCGGC	1305	0.0	61.511265	7
>>END_MODULE