FastQCFastQC Report
Tue 31 May 2016
SRR1051262_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051262_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4442880
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2212464.9797878853356385No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT353320.7952499279746471No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT211650.4763801858254106No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT150970.3398021103428407No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91610.20619508066839526No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT85490.1924202319216364No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA74700.16813418323249782No Hit
CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60100.1352726159608182No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51640.11623091328147508No Hit
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46320.10425669835782195No Hit
ATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46000.10353644482857965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG22800.067.0810852
ATATGGA42550.055.9333651
ATATCTC28600.054.8126371
ATCTCGA37400.047.498413
TCTCGAG40000.047.3791284
TATGGAT52300.045.6820262
GGATCCG66100.045.6295745
ATGGATC65150.042.5772363
CACTACG6100.042.04738
CTCGAGG47100.041.1446575
TCGAGGG47650.040.570066
GATCCGG74750.039.9045686
ATCCGGC78350.037.94987
TGGATCC80400.036.2732544
TCCGGCG86100.034.5890438
CGAGGGC56750.034.148257
AGGGCGC62950.030.9358679
CCGGCGC104850.028.312999
AATTCCC27550.028.2784141
AATTCGA11000.028.0707171