##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051262_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4442880 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.443058556611927 31.0 31.0 34.0 28.0 34.0 2 29.085392583189282 31.0 28.0 34.0 16.0 34.0 3 29.057470154494382 31.0 28.0 34.0 16.0 34.0 4 34.05286210746183 37.0 33.0 37.0 28.0 37.0 5 35.13947934673005 37.0 35.0 37.0 32.0 37.0 6 35.59014490600691 37.0 35.0 37.0 33.0 37.0 7 35.79535346441948 37.0 35.0 37.0 35.0 37.0 8 35.90451981597522 37.0 35.0 37.0 35.0 37.0 9 37.698699042062806 39.0 37.0 39.0 35.0 39.0 10-11 37.72286062193892 39.0 37.5 39.0 35.0 39.0 12-13 37.73335280268655 39.0 38.0 39.0 35.0 39.0 14-15 39.15439703525641 40.0 39.0 41.0 36.0 41.0 16-17 39.078502682944396 40.0 38.5 41.0 36.0 41.0 18-19 38.85501960440075 40.0 38.0 41.0 35.0 41.0 20-21 38.72379672644771 40.0 38.0 41.0 35.0 41.0 22-23 38.64747922518727 40.0 38.0 41.0 35.0 41.0 24-25 38.58963059997119 40.0 38.0 41.0 35.0 41.0 26-27 38.4382384849467 40.0 37.5 41.0 34.0 41.0 28-29 38.29477602366033 40.0 37.0 41.0 34.0 41.0 30-31 38.19028625576203 40.0 37.0 41.0 34.0 41.0 32-33 38.032738561473636 40.0 36.0 41.0 34.0 41.0 34-35 37.89086324636272 40.0 36.0 41.0 33.0 41.0 36-37 37.77388867131231 40.0 35.5 41.0 33.0 41.0 38-39 37.665545772111784 40.0 35.0 41.0 33.0 41.0 40-41 37.54386951707001 39.5 35.0 41.0 33.0 41.0 42-43 37.40955686401613 39.0 35.0 41.0 33.0 41.0 44-45 37.254010799301355 39.0 35.0 41.0 33.0 41.0 46-47 37.07153029116249 39.0 35.0 40.0 32.5 41.0 48-49 36.8647694738548 38.5 35.0 40.0 32.0 41.0 50-51 36.67806602924229 38.0 35.0 40.0 31.0 41.0 52-53 36.635148597306255 38.0 35.0 40.0 31.0 41.0 54-55 36.61120478158311 39.0 35.0 40.5 31.0 41.0 56-57 36.490372348566694 38.0 35.0 41.0 31.0 41.0 58-59 36.248367950518585 38.0 35.0 40.0 31.0 41.0 60-61 35.94930765629502 37.0 35.0 40.0 31.0 41.0 62-63 35.617819972630365 37.0 35.0 40.0 31.0 41.0 64-65 35.26921242527369 36.0 35.0 39.5 30.0 41.0 66-67 34.901180315471045 35.5 34.0 39.0 30.0 41.0 68-69 34.51779712708874 35.0 34.0 39.0 29.0 41.0 70-71 34.16228358632239 35.0 34.0 37.5 29.0 40.0 72-73 33.76262098008499 35.0 34.0 37.0 29.0 39.5 74-75 33.38106228392394 35.0 34.0 37.0 28.5 39.0 76-77 32.614381212186686 35.0 33.0 36.0 27.0 39.0 78-79 32.68823026955488 35.0 33.0 36.0 27.0 37.5 80-81 32.46540273426246 35.0 33.0 35.0 27.0 37.0 82-83 32.19293262478392 35.0 33.0 35.0 26.5 37.0 84-85 31.897951779026215 35.0 33.0 35.0 25.5 36.0 86-87 31.616400060321233 35.0 33.0 35.0 25.0 36.0 88-89 31.33476416198502 35.0 33.0 35.0 24.0 36.0 90-91 31.064010619237973 35.0 33.0 35.0 21.5 35.5 92-93 30.79774538137424 35.0 33.0 35.0 19.0 35.0 94-95 30.520286165730337 35.0 33.0 35.0 11.5 35.0 96-97 30.227707928190725 35.0 32.0 35.0 2.0 35.0 98-99 29.926937707072888 35.0 32.0 35.0 2.0 35.0 100-101 29.38097574096082 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.1248911217803261 1101 2 3.642760343909355 1101 3 3.7081486359394553 1101 4 2.1940371507585965 1101 5 1.0509880960456215 1101 6 0.5696980822514348 1101 7 0.3745697134131589 1101 8 0.31676275944963805 1101 9 0.37752226495596375 1101 10-11 0.31114179787514473 1101 12-13 0.3189155513082582 1101 14-15 0.36430683619258986 1101 16-17 0.33016542369253443 1101 18-19 0.42215902518974957 1101 20-21 0.4152471756669982 1101 22-23 0.44067538780746673 1101 24-25 0.429944154672782 1101 26-27 0.4552317092057905 1101 28-29 0.47849194992349453 1101 30-31 0.45364354534438434 1101 32-33 0.5349071890445742 1101 34-35 0.5282282001434169 1101 36-37 0.5094151939029032 1101 38-39 0.49389360955802175 1101 40-41 0.47403097831164587 1101 42-43 0.4933447381597702 1101 44-45 0.36569176782189317 1101 46-47 0.41394955164620484 1101 48-49 0.4686886994065915 1101 50-51 0.4041765091402354 1101 52-53 0.3957937380755894 1101 54-55 0.5010874183081455 1101 56-57 0.43003853486646904 1101 58-59 0.4936411383087673 1101 60-61 0.4194716893580761 1101 62-63 0.41921100334567285 1101 64-65 0.4037151571572011 1101 66-67 0.4255743069820639 1101 68-69 0.36681775425909535 1101 70-71 0.4067415592876671 1101 72-73 0.29282741472385254 1101 74-75 0.30948733033931575 1101 76-77 0.31143683281386103 1101 78-79 0.3290834442515944 1101 80-81 0.351508618167955 1101 82-83 0.2783987945348301 1101 84-85 0.24013955858530167 1101 86-87 0.23737720619234182 1101 88-89 0.223394316562203 1101 90-91 0.2190049519528543 1101 92-93 0.1970120905799675 1101 94-95 0.2328684954168807 1101 96-97 0.1752299427860713 1101 98-99 0.197363480389118 1101 100-101 0.24875036044796772 1102 1 1.1197545167429261 1102 2 3.648341366000235 1102 3 3.7127097110006737 1102 4 2.1563453329123945 1102 5 1.0678572093781398 1102 6 0.6218262602110585 1102 7 0.4426818575872602 1102 8 0.36436589292259214 1102 9 0.32810792066266004 1102 10-11 0.3096256660696568 1102 12-13 0.29095348736663595 1102 14-15 0.3486822464825181 1102 16-17 0.2808375138099777 1102 18-19 0.3109365983213266 1102 20-21 0.3670104196608932 1102 22-23 0.4179819111160086 1102 24-25 0.4144104507381101 1102 26-27 0.46943911630056334 1102 28-29 0.5267673893376354 1102 30-31 0.5384186012517276 1102 32-33 0.4134233774438272 1102 34-35 0.5678803420321827 1102 36-37 0.5705682963871652 1102 38-39 0.5848621173970443 1102 40-41 0.5293445498240601 1102 42-43 0.45118596838387504 1102 44-45 0.4642205962982544 1102 46-47 0.3907167254307282 1102 48-49 0.49232493616475637 1102 50-51 0.5445561239164363 1102 52-53 0.45234026265989513 1102 54-55 0.5139858244378317 1102 56-57 0.5276442222536915 1102 58-59 0.48446061086192316 1102 60-61 0.5050052608009423 1102 62-63 0.5344457134626097 1102 64-65 0.5209366896760272 1102 66-67 0.5396275919453046 1102 68-69 0.5551402900105984 1102 70-71 0.5391825473815075 1102 72-73 0.5056516704107139 1102 74-75 0.46393301763541217 1102 76-77 0.3973358208283386 1102 78-79 0.3706740880046553 1102 80-81 0.3487773537369847 1102 82-83 0.37151200583743105 1102 84-85 0.4185112110241853 1102 86-87 0.4346443770392625 1102 88-89 0.4199929733711585 1102 90-91 0.5271650287813081 1102 92-93 0.4920815882226357 1102 94-95 0.4745273724451913 1102 96-97 0.5124066073476605 1102 98-99 0.4654367852824812 1102 100-101 0.5109895899140469 1103 1 1.0027114584589576 1103 2 3.49424197940964 1103 3 3.599204398536873 1103 4 2.150939972138758 1103 5 1.0265509866375453 1103 6 0.528658020736863 1103 7 0.39170461779850285 1103 8 0.2868217658695045 1103 9 0.22653964164624796 1103 10-11 0.1882717514895873 1103 12-13 0.16354767862191721 1103 14-15 0.2633794208086968 1103 16-17 0.26400571951893426 1103 18-19 0.28901111201817997 1103 20-21 0.30855162576544615 1103 22-23 0.36795381503227986 1103 24-25 0.258514937865975 1103 26-27 0.2947155386924649 1103 28-29 0.4148018639871225 1103 30-31 0.35525744510044177 1103 32-33 0.44880807814733714 1103 34-35 0.5503954173601642 1103 36-37 0.4506456519821782 1103 38-39 0.4674496276748741 1103 40-41 0.4771957200846799 1103 42-43 0.4049595402802524 1103 44-45 0.38413985566710807 1103 46-47 0.31231249275656836 1103 48-49 0.4431261030006155 1103 50-51 0.4077373752085265 1103 52-53 0.3732003662947747 1103 54-55 0.3959518840735967 1103 56-57 0.4061819184190938 1103 58-59 0.5006768137729622 1103 60-61 0.45896115789426517 1103 62-63 0.44322977159375654 1103 64-65 0.3984579741193315 1103 66-67 0.4558997302377392 1103 68-69 0.4951087666132423 1103 70-71 0.4856026035248746 1103 72-73 0.45818897348063103 1103 74-75 0.48986768626761545 1103 76-77 0.4076333958656235 1103 78-79 0.42065670032528857 1103 80-81 0.4471204211063551 1103 82-83 0.4591748160030704 1103 84-85 0.48803641302969325 1103 86-87 0.5412726112029418 1103 88-89 0.5511176330445053 1103 90-91 0.5795427669790385 1103 92-93 0.5353712704792493 1103 94-95 0.5571387751763623 1103 96-97 0.5751767257126978 1103 98-99 0.5447503314480464 1103 100-101 0.5845305854256537 1104 1 0.7924811357889787 1104 2 2.0932709071900426 1104 3 2.9746499158419972 1104 4 1.8738452925193698 1104 5 0.8557763084093182 1104 6 0.45024966535267197 1104 7 0.3012519948047725 1104 8 0.11036002839041714 1104 9 0.17014348086254927 1104 10-11 0.16485995470784331 1104 12-13 0.12029442942613144 1104 14-15 0.16253562012398248 1104 16-17 0.11235062420876574 1104 18-19 0.18904877387037544 1104 20-21 0.2369536543535702 1104 22-23 0.17048504812925103 1104 24-25 0.22514497461883565 1104 26-27 0.2203434920474976 1104 28-29 0.27441543191305584 1104 30-31 0.2638670253513098 1104 32-33 0.1952770358159981 1104 34-35 0.2416036388990861 1104 36-37 0.2827859852577248 1104 38-39 0.23513460178634205 1104 40-41 0.3316288663200453 1104 42-43 0.3312646154754191 1104 44-45 0.30634209198936446 1104 46-47 0.28417854460629854 1104 48-49 0.33990073407679944 1104 50-51 0.33914689335593806 1104 52-53 0.2710550170708359 1104 54-55 0.2615904645745033 1104 56-57 0.290042847799441 1104 58-59 0.32247020364351897 1104 60-61 0.30173698092426093 1104 62-63 0.2812107136931985 1104 64-65 0.3041530107478181 1104 66-67 0.31587535387748034 1104 68-69 0.3013360027217047 1104 70-71 0.31418743466998933 1104 72-73 0.3263478230580432 1104 74-75 0.3341631230542532 1104 76-77 0.2552290405033659 1104 78-79 0.2926941501232392 1104 80-81 0.2896545249155693 1104 82-83 0.28094938664457203 1104 84-85 0.2965904167699911 1104 86-87 0.2916161141201492 1104 88-89 0.23111699568608657 1104 90-91 0.28461921372871757 1104 92-93 0.34161394667343714 1104 94-95 0.31532373617415743 1104 96-97 0.30024096127808164 1104 98-99 0.3942420329088421 1104 100-101 0.46446125268247584 1105 1 0.1834932402781675 1105 2 -1.1697184205266034 1105 3 0.056784224097711444 1105 4 0.5506557033447663 1105 5 0.2200491300155889 1105 6 0.1362320806354731 1105 7 0.11775466689518765 1105 8 0.027692439901002786 1105 9 0.04990238051291129 1105 10-11 -0.046706678111938515 1105 12-13 -0.003527234975145177 1105 14-15 0.013099461502868337 1105 16-17 -0.022795026671886376 1105 18-19 -0.0700058589691892 1105 20-21 -0.02456808289478829 1105 22-23 -0.08400709390895855 1105 24-25 -0.0069563607461304855 1105 26-27 0.008756736612099303 1105 28-29 -0.027461450330676485 1105 30-31 -0.03077281445931135 1105 32-33 0.008317504906699469 1105 34-35 0.0049864521817397645 1105 36-37 0.013966194597237802 1105 38-39 -0.07362971872598933 1105 40-41 -0.06592500527366951 1105 42-43 0.006627508976443153 1105 44-45 -0.050132368861817156 1105 46-47 0.042930868230392605 1105 48-49 0.034147261472369905 1105 50-51 -0.0051556360086095765 1105 52-53 0.0706949991126109 1105 54-55 0.014720019836254039 1105 56-57 -0.024118105916066668 1105 58-59 -0.04096884659870881 1105 60-61 -0.04334993467577419 1105 62-63 -0.06444428548433478 1105 64-65 -0.06006839739002601 1105 66-67 -0.0724690137071704 1105 68-69 -0.011291216857209463 1105 70-71 -0.02079539246474127 1105 72-73 -0.09177437947168698 1105 74-75 -0.11802744689687472 1105 76-77 -0.060520969081771625 1105 78-79 -0.06340269321101033 1105 80-81 -0.05865339439113626 1105 82-83 -0.044444006162002836 1105 84-85 -0.05677843312419739 1105 86-87 -0.07410830622607989 1105 88-89 -0.07570460811235336 1105 90-91 -0.006918651295158895 1105 92-93 0.002135415537804164 1105 94-95 -0.029028673035988106 1105 96-97 -0.011660372355379423 1105 98-99 0.06125128288070414 1105 100-101 0.10571385149292922 1106 1 -0.5181709871503486 1106 2 -3.496560146157684 1106 3 -3.357538276536335 1106 4 -1.3047413960947125 1106 5 -0.6211750107637357 1106 6 -0.39981688871854715 1106 7 -0.2606224990435919 1106 8 -0.18729037684900618 1106 9 -0.19286006251013532 1106 10-11 -0.21413741577013212 1106 12-13 -0.1914181742718526 1106 14-15 -0.20331570768873775 1106 16-17 -0.19686510687590442 1106 18-19 -0.23768784203490867 1106 20-21 -0.252876795046852 1106 22-23 -0.30306061909283244 1106 24-25 -0.2500464605416042 1106 26-27 -0.2700910083917307 1106 28-29 -0.2938461620080801 1106 30-31 -0.3211457386831569 1106 32-33 -0.33791344169608806 1106 34-35 -0.34262270275602447 1106 36-37 -0.436638755854041 1106 38-39 -0.4126805681160235 1106 40-41 -0.33959309980598107 1106 42-43 -0.3300320183620258 1106 44-45 -0.27287408029955884 1106 46-47 -0.2750093173068251 1106 48-49 -0.2987290772970894 1106 50-51 -0.29403998121858876 1106 52-53 -0.2789865930545332 1106 54-55 -0.23886994151597207 1106 56-57 -0.27907752692081544 1106 58-59 -0.24715813225481753 1106 60-61 -0.34412002814241305 1106 62-63 -0.3076238265973217 1106 64-65 -0.19134921846318065 1106 66-67 -0.19964051347798062 1106 68-69 -0.16315205696340485 1106 70-71 -0.17347284994870193 1106 72-73 -0.21735121081989917 1106 74-75 -0.29364052943465424 1106 76-77 -0.32079251562081623 1106 78-79 -0.2626336816957817 1106 80-81 -0.2170591331545495 1106 82-83 -0.24525820369142792 1106 84-85 -0.2310590401338466 1106 86-87 -0.15446149937884002 1106 88-89 -0.2178695231035448 1106 90-91 -0.18435152958842238 1106 92-93 -0.1736689629916306 1106 94-95 -0.1673833506726332 1106 96-97 -0.2100738709209189 1106 98-99 -0.2678899269038588 1106 100-101 -0.2679867015820214 1107 1 -0.6870771186686078 1107 2 -2.4198010675931023 1107 3 -4.545593828073322 1107 4 -2.0465332750793905 1107 5 -0.9634738387877775 1107 6 -0.7469111406142304 1107 7 -0.3612934903691425 1107 8 -0.4045572783863207 1107 9 -0.3328161780260217 1107 10-11 -0.39358613005170184 1107 12-13 -0.3295866391854574 1107 14-15 -0.607890442232538 1107 16-17 -0.4928848796819949 1107 18-19 -0.4049782437506977 1107 20-21 -0.4778893647130005 1107 22-23 -0.47192691388519137 1107 24-25 -0.5289378477124487 1107 26-27 -0.5730127821329916 1107 28-29 -0.6176678240161664 1107 30-31 -0.5739090453763325 1107 32-33 -0.5670174239473695 1107 34-35 -0.5711463505217864 1107 36-37 -0.46923931550980313 1107 38-39 -0.4988426685442491 1107 40-41 -0.5745561369926477 1107 42-43 -0.5800847991156601 1107 44-45 -0.6447904723709499 1107 46-47 -0.620543020720909 1107 48-49 -0.4926094624673638 1107 50-51 -0.5948289514348843 1107 52-53 -0.6788081198694371 1107 54-55 -0.6706417809460703 1107 56-57 -0.6404712175786926 1107 58-59 -0.6385425429754221 1107 60-61 -0.654410729009065 1107 62-63 -0.6263065509649195 1107 64-65 -0.5969010050224242 1107 66-67 -0.4965262776955015 1107 68-69 -0.43763092659953173 1107 70-71 -0.35202920887999056 1107 72-73 -0.5074724197954694 1107 74-75 -0.4243153646288391 1107 76-77 -0.4911175304281912 1107 78-79 -0.40727078699494257 1107 80-81 -0.44398280814507274 1107 82-83 -0.43997433524098994 1107 84-85 -0.4663290432463363 1107 86-87 -0.49524239692110683 1107 88-89 -0.54445862670255 1107 90-91 -0.5656024374681436 1107 92-93 -0.5872860209528703 1107 94-95 -0.5750660054977992 1107 96-97 -0.5483103575463169 1107 98-99 -0.5526145867064685 1107 100-101 -0.6335180289633051 1108 1 -3.428042865206919 1108 2 -4.603322700521137 1108 3 -5.464685518084153 1108 4 -2.9426599122579873 1108 5 -1.3572757374243736 1108 6 -0.9874458238043644 1108 7 -1.1645042281738327 1108 8 -0.8390201868035518 1108 9 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-0.04247831697595217 2305 44-45 0.05165098448471639 2305 46-47 0.06843646109451385 2305 48-49 0.015326592335696887 2305 50-51 -0.040631978803453705 2305 52-53 -0.007753424405585463 2305 54-55 -0.02163457767025534 2305 56-57 0.0066125186213739084 2305 58-59 0.023727916279590033 2305 60-61 -0.010954006194189958 2305 62-63 -0.015137723091598332 2305 64-65 -0.03877515660779096 2305 66-67 -0.0012312511265051285 2305 68-69 -0.046470155438910865 2305 70-71 -0.07347723039541165 2305 72-73 -0.018712883624289134 2305 74-75 0.011775511408373518 2305 76-77 0.09904485269692742 2305 78-79 0.06405054373964703 2305 80-81 0.021047582093629558 2305 82-83 -0.034094328140831465 2305 84-85 0.0053423508460817 2305 86-87 0.033949876894180164 2305 88-89 -0.014238273060506401 2305 90-91 -0.02400109087637503 2305 92-93 -0.04030765516069934 2305 94-95 0.10808380345847723 2305 96-97 0.1489867440895445 2305 98-99 0.10749516345831367 2305 100-101 0.09504059814399568 2306 1 -0.5641828508591438 2306 2 -2.292326440737714 2306 3 -4.182860120255842 2306 4 -1.7222575934717952 2306 5 -0.7976292693577136 2306 6 -0.5031756998276009 2306 7 -0.33137404838693385 2306 8 -0.24935074835134685 2306 9 -0.1445153793523204 2306 10-11 -0.17835294617911757 2306 12-13 -0.12698592553875443 2306 14-15 -0.16266341049360733 2306 16-17 -0.23146666164667806 2306 18-19 -0.15585811864409038 2306 20-21 -0.16555978701749297 2306 22-23 -0.18056858250632501 2306 24-25 -0.222117323021628 2306 26-27 -0.24162722384710378 2306 28-29 -0.24449120784470324 2306 30-31 -0.31858186329530014 2306 32-33 -0.22428500087904268 2306 34-35 -0.23400953677618475 2306 36-37 -0.17764409473655718 2306 38-39 -0.12236005671453398 2306 40-41 -0.16184339398171232 2306 42-43 -0.15213278099088257 2306 44-45 -0.21728466120459444 2306 46-47 -0.1847259251725646 2306 48-49 -0.21247283097826397 2306 50-51 -0.1531780451789686 2306 52-53 -0.0914075559779306 2306 54-55 -0.2397566578904815 2306 56-57 -0.2938765399294425 2306 58-59 -0.3478263458040445 2306 60-61 -0.2301023237551192 2306 62-63 -0.13604731406624637 2306 64-65 -0.1619115100600439 2306 66-67 -0.12059297424350035 2306 68-69 -0.21211179750802245 2306 70-71 -0.2561897620215845 2306 72-73 -0.16524049605468605 2306 74-75 -0.21468044410551101 2306 76-77 -0.17914910565222186 2306 78-79 -0.14881580961055363 2306 80-81 -0.21065602875498968 2306 82-83 -0.21175191988575648 2306 84-85 -0.13461601814743673 2306 86-87 -0.14882303525074292 2306 88-89 -0.13548291829630088 2306 90-91 -0.22876219366065342 2306 92-93 -0.21811064021573756 2306 94-95 -0.26780574867175844 2306 96-97 -0.2161326374400332 2306 98-99 -0.22893333192759258 2306 100-101 -0.21567655494941818 2307 1 -4.129485321039272 2307 2 -4.751522365719648 2307 3 -5.165737954781296 2307 4 -8.042528762255007 2307 5 -4.281168439114435 2307 6 -2.0434475547772593 2307 7 -1.1360255487883322 2307 8 -0.8032997745290444 2307 9 -0.6160288622607197 2307 10-11 -0.4641139779617376 2307 12-13 -0.4509950742498603 2307 14-15 -0.4831590615620911 2307 16-17 -0.4552077834444219 2307 18-19 -0.5445788547657386 2307 20-21 -0.7689456155012522 2307 22-23 -0.7936063207548543 2307 24-25 -0.8145542071526677 2307 26-27 -0.8745945568406768 2307 28-29 -0.9226638562392822 2307 30-31 -0.8570173170148792 2307 32-33 -0.7704727466520396 2307 34-35 -0.7941402240660551 2307 36-37 -0.8476023735278417 2307 38-39 -0.8680488130929334 2307 40-41 -0.8673177362203717 2307 42-43 -0.8211839813577839 2307 44-45 -0.7313340264696322 2307 46-47 -0.7017824542251176 2307 48-49 -0.7529096472079218 2307 50-51 -0.7052755127145716 2307 52-53 -0.7227857746898394 2307 54-55 -0.6892919529763901 2307 56-57 -0.7681055360836666 2307 58-59 -0.8162593872122628 2307 60-61 -0.7823119847497964 2307 62-63 -0.7530810003087893 2307 64-65 -0.6832292388367094 2307 66-67 -0.6175629700301783 2307 68-69 -0.7082508059131172 2307 70-71 -0.8322206758465214 2307 72-73 -0.6372350166889902 2307 74-75 -0.5442426117391719 2307 76-77 -0.7706348755901047 2307 78-79 -0.8626144380215237 2307 80-81 -0.8956322987923357 2307 82-83 -0.8130587987449047 2307 84-85 -0.7228400477249792 2307 86-87 -0.7132424654817768 2307 88-89 -0.6996285137562168 2307 90-91 -0.8924628037432107 2307 92-93 -0.8570318499495926 2307 94-95 -0.9127491597286586 2307 96-97 -0.9160968640403233 2307 98-99 -0.8646514461209485 2307 100-101 -0.8626770586785604 2308 1 -3.6066374834979165 2308 2 -4.767895234177814 2308 3 -4.471529371945387 2308 4 -3.884343261859321 2308 5 -1.6185286099248515 2308 6 -0.953265935624863 2308 7 -0.6182619890753287 2308 8 -0.48442916591945107 2308 9 -0.517990285362913 2308 10-11 -0.4794158129768604 2308 12-13 -0.42274643739231976 2308 14-15 -0.9020027090876468 2308 16-17 -0.6796263640786862 2308 18-19 -0.8905944391312772 2308 20-21 -0.8998015844287082 2308 22-23 -0.9791211336412076 2308 24-25 -0.9169329619540676 2308 26-27 -1.0041679034780486 2308 28-29 -1.0571589569621835 2308 30-31 -1.0123613926627897 2308 32-33 -1.0040992495163223 2308 34-35 -0.9945338072941468 2308 36-37 -1.1142865998413711 2308 38-39 -1.1675873397530339 2308 40-41 -1.0814708388980705 2308 42-43 -1.1155743735033852 2308 44-45 -1.040660702357286 2308 46-47 -0.9675556490644084 2308 48-49 -1.3066375186057542 2308 50-51 -1.064735952819369 2308 52-53 -0.8704491130292382 2308 54-55 -0.935898167893356 2308 56-57 -0.884833799493741 2308 58-59 -1.0141754016554003 2308 60-61 -0.9807695519253343 2308 62-63 -0.9267762584582115 2308 64-65 -0.9687846628801466 2308 66-67 -1.0952686230309112 2308 68-69 -0.9461542092258384 2308 70-71 -0.8643894704289465 2308 72-73 -0.8853326737363147 2308 74-75 -1.0292101827144648 2308 76-77 -0.7548506400427897 2308 78-79 -0.7096310677718698 2308 80-81 -0.7822668951807827 2308 82-83 -0.7600386416755427 2308 84-85 -0.8364002919310316 2308 86-87 -0.8372555547461893 2308 88-89 -0.873874253272021 2308 90-91 -0.9036266619212405 2308 92-93 -0.9746284936534728 2308 94-95 -0.9294557923926767 2308 96-97 -0.9889466412991617 2308 98-99 -0.9954242671443332 2308 100-101 -1.0275690368999228 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 32.0 11 308.0 12 927.0 13 1847.0 14 3085.0 15 4488.0 16 6229.0 17 9103.0 18 15002.0 19 28577.0 20 28100.0 21 24752.0 22 25293.0 23 27398.0 24 30759.0 25 35287.0 26 42191.0 27 51099.0 28 62355.0 29 75336.0 30 92141.0 31 113448.0 32 144212.0 33 189709.0 34 265481.0 35 500113.0 36 627326.0 37 1006239.0 38 872364.0 39 159480.0 40 198.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 23.30760706541594 32.85734447920684 22.78218704115505 21.052861414222175 2 22.82849603984085 29.814172213185287 29.34350872193851 18.013823025035354 3 23.194697037904024 30.741221752048258 26.22769424687134 19.836386963176373 4 22.797464707577067 29.430099394987035 27.356466976375682 20.415968921060212 5 23.52773426246039 29.67624603860559 24.530574762316334 22.26544493661769 6 23.23812481993662 29.130946593200807 25.091989880437914 22.53893870642466 7 22.946084521751654 29.926579155862864 24.829570008643042 22.297766313742436 8 22.374383282915584 28.893780610774993 25.801372082973206 22.930464023336214 9 21.95584845865745 28.868436689714777 25.91195350763469 23.263761343993085 10-11 23.25240373686042 29.147585549353867 25.080589726379817 22.5194209874059 12-13 22.555109885527912 29.388919130828278 25.385384031303165 22.670586952340642 14-15 22.423090427830598 29.705168719389224 25.669678226735808 22.202062626044366 16-17 22.48745183304523 29.78260722774417 25.45446872299049 22.27547221622011 18-19 22.124950482569865 29.924542188850477 25.99835016925958 21.95215715932008 20-21 21.53966571233074 29.95800021607606 27.01512307332181 21.48721099827139 22-23 21.566236315182945 30.208625936329586 26.907602726159606 21.31753502232786 24-25 21.11293800417747 29.834319180351482 27.743490708729475 21.30925210674157 26-27 20.960466794155618 29.957599981090517 28.323966759825897 20.757966464927968 28-29 21.216652410750463 30.178712915860086 28.04509746544816 20.559537207941293 30-31 21.113085439786214 30.13857491335551 28.204855783093556 20.54348386376472 32-33 20.90239028241834 30.467234283469608 28.134951654648567 20.495423779463483 34-35 21.05796222918872 30.580203041914622 27.92578149707903 20.436053231817628 36-37 21.018864467297824 30.592484826984172 27.965249167909604 20.423401537808395 38-39 20.782498285396848 30.94913272705296 27.93082344393152 20.33754554361867 40-41 20.88940450658268 31.167592707641166 27.636346815625558 20.306655970150597 42-43 20.786425367985213 31.168575396722236 27.683276292572234 20.361722942720316 44-45 20.687572025352924 31.611612287525208 27.480733218092766 20.220082469029098 46-47 20.764644256935792 31.832845229886537 27.185495703065342 20.21701481011232 48-49 20.68224033491921 32.036249152432696 27.139349912359428 20.142160600288666 50-51 20.59228019219502 32.35223944292525 27.016086102864893 20.039394262014834 52-53 20.654242440543623 32.618365253701484 26.720410346534308 20.00698195922059 54-55 20.57474964154116 32.777577696094426 26.6183882069855 20.02928445537891 56-57 20.432951148804378 33.15728761524056 26.546305549553445 19.86345568640161 58-59 20.509842137966064 33.48192887289364 26.107924929128256 19.90030406001204 60-61 20.360655700806685 33.601110090751945 26.102629375540193 19.935604832901184 62-63 20.318655916882744 33.95765809564967 25.9352829696053 19.788403017862287 64-65 20.333089157383647 34.197064959318205 25.665507009095208 19.804338874202944 66-67 20.172402577890757 34.38836766522083 25.64291400781074 19.796315749077678 68-69 20.082424013252663 34.77220856741573 25.474860901037168 19.670506518294438 70-71 20.225158005617978 34.969749351771824 25.170114880437914 19.634977762172284 72-73 20.101578255545952 35.16065029890522 25.11179685249208 19.625974593056757 74-75 19.902732011668107 35.57295042855085 24.895585746182654 19.62873181359839 76-77 20.025377682944395 35.823801228032266 24.57467903702103 19.576142052002304 78-79 19.85901037165082 36.0234915190147 24.565889693171997 19.551608416162487 80-81 19.650204371938923 36.44664046744454 24.37058844713339 19.532566713483146 82-83 19.871007094497262 36.744611603284355 23.880275857101697 19.504105445116682 84-85 19.715623649524634 36.82802596513973 23.8692019590896 19.58714842624604 86-87 19.614326743013542 37.183651145203115 23.730800741861135 19.471221369922212 88-89 19.653850655430713 37.47816731489485 23.332466778305964 19.535515251368484 90-91 19.618659518150388 37.53833099250936 23.24509102203976 19.59791846730049 92-93 19.598503673293 37.90362332541054 23.014677416450592 19.483195584845866 94-95 19.63547293647364 38.08122209017574 22.74810483290118 19.535200140449437 96-97 19.503666540622298 38.30998136343993 22.60442550777874 19.581926588159032 98-99 19.544462150677038 38.47972486315183 22.417294637712477 19.558518348458655 100-101 19.579101843849035 38.622593903053875 22.07954074834342 19.71876350475367 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 211720.0 1 121194.0 2 21475.5 3 10269.5 4 7993.5 5 7881.5 6 8199.0 7 8581.5 8 9160.0 9 9762.5 10 10861.0 11 11816.5 12 12693.5 13 15170.5 14 20744.0 15 26024.0 16 35671.5 17 34444.5 18 23723.0 19 22709.5 20 23748.0 21 25036.5 22 26540.0 23 28968.5 24 31090.5 25 33021.5 26 36250.0 27 40222.0 28 44276.5 29 49252.5 30 55440.0 31 60129.0 32 64826.0 33 70400.5 34 73996.5 35 77468.5 36 82364.0 37 86549.0 38 89474.0 39 92617.0 40 94587.0 41 95855.5 42 97913.0 43 100943.5 44 104520.0 45 109634.5 46 117066.5 47 126299.5 48 135457.5 49 143736.5 50 146120.5 51 141838.0 52 138384.0 53 137274.0 54 135960.5 55 132644.5 56 124631.0 57 115257.5 58 106527.0 59 96384.5 60 85275.0 61 73443.0 62 62672.0 63 51305.0 64 38676.0 65 27561.0 66 20125.5 67 15668.5 68 12140.5 69 9241.0 70 7310.5 71 5347.0 72 3991.0 73 3193.5 74 2653.5 75 2453.5 76 2113.0 77 1390.0 78 728.5 79 372.5 80 160.5 81 59.5 82 30.5 83 14.5 84 7.5 85 3.5 86 2.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007630185825410544 2 6.752376836646499E-5 3 0.0018006338231057331 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.3504753673292998E-4 12-13 1.4630149812734083E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.014967768654566407 28-29 0.0063022183808700664 30-31 0.006774884759435321 32-33 0.014011181936041485 34-35 0.007855265053298761 36-37 0.010218596946125036 38-39 0.0036237755690002884 40-41 0.00702247191011236 42-43 1.012856525496975E-4 44-45 0.0 46-47 7.877772976087583E-5 48-49 3.9388864880437915E-4 50-51 0.0022733002016709883 52-53 2.2507922788821664E-5 54-55 0.005626980697205416 56-57 0.0 58-59 0.0038601087582829157 60-61 0.0 62-63 0.0 64-65 0.0010241104868913859 66-67 5.176822241428984E-4 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4442880.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.97005498788732 #Duplication Level Percentage of deduplicated Percentage of total 1 79.86192440328173 40.7056667827377 2 9.954683735180177 10.147815547383225 3 3.228164042860713 4.936190963235936 4 1.6595378776398935 3.3834694751114878 5 1.0419547604299517 2.65542457170028 6 0.7230595543283835 2.2112631146181005 7 0.5393218985309393 1.924248677700563 8 0.4314701158548818 1.759364442460275 9 0.3559262166595322 1.6327420951290348 >10 2.133787506493995 19.05727327452369 >50 0.05930209745460381 1.966957130785807 >100 0.008394656412977063 0.6874713747729019 >500 7.949362692350801E-4 0.29366505527362097 >1k 0.001280730512913666 1.2283013762109336 >5k 2.2081560567474123E-4 0.8183273449295198 >10k+ 1.76652484539793E-4 6.5918187734268745 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 221246 4.9797878853356385 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35332 0.7952499279746471 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 21165 0.4763801858254106 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 15097 0.3398021103428407 No Hit GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9161 0.20619508066839526 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8549 0.1924202319216364 No Hit AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7470 0.16813418323249782 No Hit CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6010 0.1352726159608182 No Hit ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5164 0.11623091328147508 No Hit CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4632 0.10425669835782195 No Hit ATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4600 0.10353644482857965 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0034437121866897147 0.0 0.0 0.0 0.0 2 0.0034437121866897147 0.0 0.0 0.0 0.0 3 0.0034437121866897147 0.0 0.0 0.0 0.0 4 0.0034662201094785364 0.0 0.0 0.0 4.501584557764333E-5 5 0.0035112359550561797 0.0 0.0 0.0 4.501584557764333E-5 6 0.0035112359550561797 0.0 0.0 0.0 6.7523768366465E-5 7 0.0035337438778450013 0.0 0.0 0.0 6.7523768366465E-5 8 0.003556251800633823 0.0 0.0 0.0 6.7523768366465E-5 9 0.003556251800633823 2.2507922788821664E-5 0.0 0.0 9.003169115528665E-5 10-11 0.003556251800633823 2.2507922788821664E-5 0.0 0.0 1.800633823105733E-4 12-13 0.0036350295303946988 2.2507922788821664E-5 0.0 0.0 1.9131734370498416E-4 14-15 0.0036687914145779313 2.2507922788821664E-5 0.0 0.0 2.3633318928262747E-4 16-17 0.003758823105733218 2.2507922788821664E-5 0.0 0.0 2.475871506770383E-4 18-19 0.003927632526649381 2.2507922788821664E-5 0.0 1.1253961394410832E-5 2.588411120714491E-4 20-21 0.004152711754537597 2.2507922788821664E-5 0.0 2.2507922788821664E-5 2.7009507346586E-4 22-23 0.004366537021031403 2.2507922788821664E-5 0.0 2.2507922788821664E-5 3.151109190435033E-4 24-25 0.004647886055891674 2.2507922788821664E-5 0.0 2.2507922788821664E-5 3.3761884183232496E-4 26-27 0.004951743013540766 2.2507922788821664E-5 0.0 2.2507922788821664E-5 3.826346874099683E-4 28-29 0.00527810789397868 2.2507922788821664E-5 0.0 2.2507922788821664E-5 4.501584557764333E-4 30-31 0.00541315543071161 2.2507922788821664E-5 0.0 2.2507922788821664E-5 4.951743013540766E-4 32-33 0.005469425237683664 2.2507922788821664E-5 0.0 2.2507922788821664E-5 5.289361855373091E-4 34-35 0.005604472774416594 2.2507922788821664E-5 0.0 2.2507922788821664E-5 5.964599539037741E-4 36-37 0.005919583693460098 2.2507922788821664E-5 0.0 2.2507922788821664E-5 6.302218380870066E-4 38-39 0.006020869346009795 2.2507922788821664E-5 0.0 2.2507922788821664E-5 6.639837222702391E-4 40-41 0.006144662921348315 2.2507922788821664E-5 0.0 2.2507922788821664E-5 7.315074906367042E-4 42-43 0.00634723422644771 2.2507922788821664E-5 0.0 2.2507922788821664E-5 8.778089887640449E-4 44-45 0.0065835674157303375 2.2507922788821664E-5 0.0 2.2507922788821664E-5 9.115708729472774E-4 46-47 0.006797392682224143 2.2507922788821664E-5 0.0 2.2507922788821664E-5 9.453327571305099E-4 48-49 0.007044979832901181 2.2507922788821664E-5 0.0 2.2507922788821664E-5 9.903486027081532E-4 50-51 0.007292566983578219 2.2507922788821664E-5 0.0 2.2507922788821664E-5 0.001001602564102564 52-53 0.008395455200230481 2.2507922788821664E-5 0.0 2.2507922788821664E-5 0.0010128565254969748 54-55 0.00973467660616537 2.2507922788821664E-5 0.0 2.2507922788821664E-5 0.0010241104868913857 56-57 0.011749135695764908 2.2507922788821664E-5 0.0 2.2507922788821664E-5 0.00108038029386344 58-59 0.013696071016997984 2.2507922788821664E-5 0.0 2.2507922788821664E-5 0.00108038029386344 60-61 0.016149434600979545 2.2507922788821664E-5 0.0 2.2507922788821664E-5 0.00108038029386344 62-63 0.018625306107749928 2.2507922788821664E-5 0.0 2.2507922788821664E-5 0.00108038029386344 64-65 0.02188895491212907 2.2507922788821664E-5 0.0 2.2507922788821664E-5 0.0011141421780466725 66-67 0.02634552362431576 2.2507922788821664E-5 0.0 2.2507922788821664E-5 0.001147904062229905 68-69 0.0320062662057044 2.2507922788821664E-5 0.0 2.2507922788821664E-5 0.0011591580236243158 70-71 0.038105913281475084 2.2507922788821664E-5 0.0 2.2507922788821664E-5 0.0011704119850187266 72-73 0.04564606741573034 2.2507922788821664E-5 0.0 2.2507922788821664E-5 0.0011929199078075483 74-75 0.05374891961970614 2.2507922788821664E-5 0.0 2.2507922788821664E-5 0.0011929199078075483 76-77 0.06459773840391818 2.2507922788821664E-5 0.0 2.2507922788821664E-5 0.0011929199078075483 78-79 0.07658320728896571 2.2507922788821664E-5 0.0 2.2507922788821664E-5 0.0012041738692019591 80-81 0.0905943892250072 2.2507922788821664E-5 0.0 2.2507922788821664E-5 0.0012604436761740132 82-83 0.10776793431287812 2.2507922788821664E-5 0.0 2.2507922788821664E-5 0.0012942055603572457 84-85 0.1295556035724575 2.2507922788821664E-5 0.0 2.2507922788821664E-5 0.0013167134831460674 86-87 0.15392042999135697 4.501584557764333E-5 0.0 2.2507922788821664E-5 0.0013279674445404782 88-89 0.1834620786516854 4.501584557764333E-5 0.0 2.2507922788821664E-5 0.001339221405934889 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCTCG 2280 0.0 67.081085 2 ATATGGA 4255 0.0 55.933365 1 ATATCTC 2860 0.0 54.812637 1 ATCTCGA 3740 0.0 47.49841 3 TCTCGAG 4000 0.0 47.379128 4 TATGGAT 5230 0.0 45.682026 2 GGATCCG 6610 0.0 45.629574 5 ATGGATC 6515 0.0 42.577236 3 CACTACG 610 0.0 42.0473 8 CTCGAGG 4710 0.0 41.144657 5 TCGAGGG 4765 0.0 40.57006 6 GATCCGG 7475 0.0 39.904568 6 ATCCGGC 7835 0.0 37.9498 7 TGGATCC 8040 0.0 36.273254 4 TCCGGCG 8610 0.0 34.589043 8 CGAGGGC 5675 0.0 34.14825 7 AGGGCGC 6295 0.0 30.935867 9 CCGGCGC 10485 0.0 28.31299 9 AATTCCC 2755 0.0 28.278414 1 AATTCGA 1100 0.0 28.070717 1 >>END_MODULE