FastQCFastQC Report
Tue 31 May 2016
SRR1051261_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051261_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7581462
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT175590523.1605065091667No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1123311.481653538591897No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT796081.0500349404903697No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT488810.6447437182960226No Hit
GTCGACGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT445250.5872877816969867No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328370.4331222658637608No Hit
CTATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT191350.25239195289773925No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT167930.2215008134314991No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152920.2017025212287551No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151820.20025161373888045No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTAAAAAAAAAA108530.14315180897826832No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107710.1420702233949072No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTAAAAAAAAA104980.13846933480640014No Hit
ATATCTCGAGGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTT97800.12899886591794563No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTAAAAAAAA91970.12130905622161003No Hit
ATATCTCGAGGGCGCGCCGTATCCTTTTTTTTTTTTTTTTTTTTTTTTTT91680.1209265442470067No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTAAAAAAAAAAA86990.11474040231290482No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTAAAAAAA81450.10743310459117252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG726300.094.504751
ATATCTC2033450.094.2155461
TATCTCG2064050.093.649462
TCGACGG756350.090.7374952
ATCTCGA2132300.090.587353
TCTCGAG2118850.090.254574
CTCGAGG2134900.089.2868355
TCGAGGG2125650.089.219586
CGAGGGC2131200.088.773297
ACGGCGC769150.088.708765
CGACGGC770200.088.64953
GACGGCG770800.088.592814
AGGGCGC2160450.087.590739
GAGGGCG2164250.087.496648
CGGCGCG776950.087.384186
CTATCTC40500.055.9377631
CGCGCCT23350.050.037849
TTCGAAC16900.049.7425773
TCTCGAC36000.048.0220384
GTCGACT9500.047.9942671
Produced by FastQC (version 0.11.5)