##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1051261_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7581462
Sequences flagged as poor quality	0
Sequence length	101
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.796966732801668	31.0	31.0	33.0	28.0	34.0
2	31.54488369129859	31.0	31.0	34.0	30.0	34.0
3	32.01232941614691	33.0	31.0	34.0	30.0	34.0
4	35.59639341330208	37.0	35.0	37.0	33.0	37.0
5	35.642233516437855	37.0	35.0	37.0	35.0	37.0
6	35.75243323781086	37.0	35.0	37.0	35.0	37.0
7	35.558125728256634	37.0	35.0	37.0	33.0	37.0
8	35.57167298339027	37.0	35.0	37.0	33.0	37.0
9	37.00565787970711	39.0	37.0	39.0	33.0	39.0
10-11	36.82570716571553	39.0	37.0	39.0	33.0	39.0
12-13	36.67260232920775	39.0	37.0	39.0	32.0	39.0
14-15	38.009832470307174	40.0	38.0	41.0	32.5	41.0
16-17	37.72398733911744	40.0	37.5	41.0	31.5	41.0
18-19	37.90875941869787	40.0	38.0	41.0	32.5	41.0
20-21	37.283450935980426	39.5	37.0	41.0	31.0	41.0
22-23	37.37312598810098	39.5	37.0	41.0	31.5	41.0
24-25	37.75399652468086	40.0	37.5	41.0	32.5	41.0
26-27	38.15451149131922	40.0	38.0	41.0	33.5	41.0
28-29	38.12873222869151	40.0	38.0	41.0	33.5	41.0
30-31	37.43223727824528	39.5	36.0	40.5	33.0	41.0
32-33	36.3692745541691	36.0	35.0	40.0	33.0	41.0
34-35	36.064863742639616	35.0	35.0	40.0	33.0	41.0
36-37	36.08719663832649	35.0	35.0	40.0	33.0	41.0
38-39	35.745876784715136	35.0	35.0	40.0	32.0	41.0
40-41	34.16024877523623	35.0	34.5	40.0	22.0	41.0
42-43	34.01722675652796	35.0	33.5	40.0	22.0	41.0
44-45	34.24194647417609	35.0	34.0	40.0	23.0	41.0
46-47	34.38493683672094	35.0	33.0	40.0	23.0	41.0
48-49	33.84833551892761	35.0	33.0	40.0	23.0	41.0
50-51	33.67505198865338	35.0	32.5	39.0	23.0	40.5
52-53	33.41821603801483	35.0	33.0	38.0	23.0	40.0
54-55	33.29318718474089	35.0	33.0	38.0	23.0	40.0
56-57	33.00651444272886	35.0	33.0	38.0	23.0	40.0
58-59	32.52491564291953	35.0	32.5	38.0	21.0	40.0
60-61	32.09575547565892	35.0	32.0	37.5	20.0	40.0
62-63	31.68819134093134	35.0	31.0	37.0	18.0	40.0
64-65	31.509373521887994	35.0	31.0	36.5	18.0	39.5
66-67	31.086321873010775	35.0	31.0	36.0	14.0	39.0
68-69	30.835582042091616	35.0	31.0	36.0	12.0	39.0
70-71	30.7007835032346	35.0	31.0	35.5	9.5	39.0
72-73	30.391227378043972	35.0	30.5	35.0	7.0	38.5
74-75	29.86982253554789	35.0	30.0	35.0	3.5	37.0
76-77	29.687431065406646	35.0	30.0	35.0	2.0	37.0
78-79	29.110465830996713	34.0	29.0	35.0	2.0	36.0
80-81	29.033225715567788	34.5	29.5	35.0	2.0	36.0
82-83	28.761099442297542	34.0	29.0	35.0	2.0	35.5
84-85	28.5126039674142	34.0	29.0	35.0	2.0	35.0
86-87	28.214031277872262	34.0	29.0	35.0	2.0	35.0
88-89	28.204526778608134	34.0	29.0	35.0	2.0	35.0
90-91	27.905201134029294	34.0	29.0	35.0	2.0	35.0
92-93	27.50530893645579	34.0	27.0	35.0	2.0	35.0
94-95	27.18473409218433	34.0	27.0	35.0	2.0	35.0
96-97	27.00977067483818	34.0	27.0	35.0	2.0	35.0
98-99	26.550025575542026	34.0	25.0	35.0	2.0	35.0
100-101	25.620253191271026	33.5	21.5	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.4982066762871824
1101	2	0.35156717630152556
1101	3	0.42227937424708983
1101	4	0.2910287564520999
1101	5	0.39123626221333296
1101	6	0.31535000723277307
1101	7	0.4550525246942243
1101	8	0.47193710163613645
1101	9	0.6185213069309796
1101	10-11	0.5743780944489814
1101	12-13	0.6792697166067398
1101	14-15	0.7889542497415221
1101	16-17	0.7885185680407645
1101	18-19	0.6676415361554504
1101	20-21	0.8545448205860637
1101	22-23	0.8595204767264946
1101	24-25	0.889815039046816
1101	26-27	0.7613584036124124
1101	28-29	0.7459434294736411
1101	30-31	0.7913067982736948
1101	32-33	0.8336501479426204
1101	34-35	0.7707452692443582
1101	36-37	0.6905748013478288
1101	38-39	0.766131021240092
1101	40-41	1.1161006338853738
1101	42-43	1.188319337737731
1101	44-45	0.8441682730926559
1101	46-47	0.8465213924134929
1101	48-49	0.9511656471096117
1101	50-51	0.8662041354463312
1101	52-53	0.6649830260188523
1101	54-55	0.5019338755009741
1101	56-57	0.25843175405262997
1101	58-59	0.37826680445051153
1101	60-61	0.5496540864712145
1101	62-63	0.6105056653919902
1101	64-65	0.45697195720179096
1101	66-67	0.40211233581971584
1101	68-69	0.37191051201313385
1101	70-71	0.47834361016164806
1101	72-73	0.4930967348992965
1101	74-75	0.37648253575151713
1101	76-77	0.3425491069253255
1101	78-79	0.17703012086565195
1101	80-81	0.21424599134123667
1101	82-83	0.24639739674836392
1101	84-85	0.2804318879327923
1101	86-87	0.1886649887838594
1101	88-89	0.21620573169056456
1101	90-91	0.2745095951774097
1101	92-93	0.31664664144959076
1101	94-95	0.3706032119741103
1101	96-97	0.35975758778010203
1101	98-99	0.36417317686075634
1101	100-101	0.4576137136258609
1102	1	0.15705151843551945
1102	2	0.26942724417180486
1102	3	0.35460918495863325
1102	4	0.21696914159522152
1102	5	0.33695373129498307
1102	6	0.24824104921208345
1102	7	0.40852202247992864
1102	8	0.4502748824024181
1102	9	0.5416423977648677
1102	10-11	0.4724657384537565
1102	12-13	0.5804242704774722
1102	14-15	0.7141939338927372
1102	16-17	0.8380150986828951
1102	18-19	0.7070735447342003
1102	20-21	0.9440219545746587
1102	22-23	0.875030989527076
1102	24-25	0.791254405941821
1102	26-27	0.654729796055797
1102	28-29	0.5921879079312617
1102	30-31	0.6160351211419552
1102	32-33	0.6792868913716603
1102	34-35	0.670421272264818
1102	36-37	0.6632373861562328
1102	38-39	0.7054111480095884
1102	40-41	1.1177932128576131
1102	42-43	1.12982275154598
1102	44-45	0.7788918790915176
1102	46-47	0.730057830956838
1102	48-49	0.9012145183094376
1102	50-51	0.6946746382507598
1102	52-53	0.4212884414420657
1102	54-55	0.3497797318108624
1102	56-57	0.1727014517615686
1102	58-59	0.17273008358763775
1102	60-61	0.3319417473486652
1102	62-63	0.37596274111858463
1102	64-65	0.12969701524837873
1102	66-67	0.2004986871806338
1102	68-69	0.14507018697719687
1102	70-71	0.19803889901344718
1102	72-73	0.31211470624095483
1102	74-75	0.2482633721293439
1102	76-77	0.2792814094183669
1102	78-79	0.333131870041516
1102	80-81	0.28998107422445685
1102	82-83	0.23057176105497135
1102	84-85	0.24191333678329485
1102	86-87	0.09205677666575696
1102	88-89	0.24614163629260943
1102	90-91	0.4877064897734549
1102	92-93	0.4797048019037682
1102	94-95	0.4802054836073282
1102	96-97	0.44927805878548455
1102	98-99	0.3877217448926267
1102	100-101	0.6052892611671261
1103	1	0.37464937046259195
1103	2	0.3027197416050882
1103	3	0.41077971492322973
1103	4	0.33998925928415247
1103	5	0.42422259946994956
1103	6	0.3260216308757862
1103	7	0.41661913232410797
1103	8	0.4319060896044107
1103	9	0.5986688692504885
1103	10-11	0.5338965367748401
1103	12-13	0.609709479433775
1103	14-15	0.7420867110934282
1103	16-17	0.7888758920106866
1103	18-19	0.6725070074206769
1103	20-21	0.914498557707283
1103	22-23	0.9073033187889834
1103	24-25	0.8238152444706017
1103	26-27	0.6773070858137018
1103	28-29	0.5947378534938395
1103	30-31	0.5936291437544838
1103	32-33	0.6024771278947227
1103	34-35	0.5523319862385634
1103	36-37	0.5494274566950494
1103	38-39	0.5804231500941626
1103	40-41	0.8466257133066719
1103	42-43	0.8229411910906705
1103	44-45	0.6299537000925284
1103	46-47	0.5235317495609095
1103	48-49	0.6884952410599965
1103	50-51	0.5330927551622011
1103	52-53	0.3508643423651634
1103	54-55	0.34886636878764676
1103	56-57	0.26235073718172686
1103	58-59	0.30223541920218366
1103	60-61	0.28324498823392474
1103	62-63	0.3814757102488642
1103	64-65	0.19068583486690827
1103	66-67	0.17315243611394848
1103	68-69	0.23384613756141093
1103	70-71	0.3167668808355728
1103	72-73	0.40778884215422195
1103	74-75	0.33776583383160386
1103	76-77	0.3260069149556415
1103	78-79	0.4740533730591707
1103	80-81	0.3908866114581535
1103	82-83	0.508739485593761
1103	84-85	0.5839323916334465
1103	86-87	0.5753500817316741
1103	88-89	0.5283178683311434
1103	90-91	0.6575049445325085
1103	92-93	0.5996107828857937
1103	94-95	0.6805957257335749
1103	96-97	0.6757762893342729
1103	98-99	0.7202922577014554
1103	100-101	0.8440999269709586
1104	1	0.006011017281068121
1104	2	0.131272941814899
1104	3	0.19632131571407996
1104	4	0.030969978438321277
1104	5	0.10854895866352621
1104	6	0.058130092217844265
1104	7	0.27245756422985323
1104	8	0.18490830322734553
1104	9	0.32309886099380236
1104	10-11	0.2535660592416633
1104	12-13	0.4811447657743244
1104	14-15	0.5432342464695594
1104	16-17	0.5365715864510676
1104	18-19	0.37691016122820287
1104	20-21	0.5625395306431002
1104	22-23	0.47520392084100394
1104	24-25	0.4200850194815473
1104	26-27	0.3589143294170043
1104	28-29	0.3675343105450253
1104	30-31	0.4027092163208721
1104	32-33	0.38444334584934126
1104	34-35	0.37818867613044205
1104	36-37	0.2674674725694146
1104	38-39	0.21934582008644554
1104	40-41	0.5039057733021437
1104	42-43	0.47100913048828374
1104	44-45	0.39156054960372444
1104	46-47	0.33206654890242504
1104	48-49	0.3413888728398078
1104	50-51	0.2211594570326767
1104	52-53	0.13815335584186528
1104	54-55	0.004576013364335552
1104	56-57	-0.050539208011706194
1104	58-59	0.04947452473009406
1104	60-61	0.16168712552309472
1104	62-63	0.2525286497949537
1104	64-65	0.20919984274709336
1104	66-67	0.10611281800451522
1104	68-69	0.18179783654111503
1104	70-71	0.31752834995210577
1104	72-73	0.2698844917889218
1104	74-75	0.16761876587264268
1104	76-77	0.16085430563673242
1104	78-79	0.2652780249077473
1104	80-81	0.292060848816881
1104	82-83	0.33727550144899965
1104	84-85	0.3394890757543365
1104	86-87	0.19633324564275867
1104	88-89	0.22343309473853523
1104	90-91	0.27822316957965754
1104	92-93	0.3380478736039585
1104	94-95	0.44928657350664736
1104	96-97	0.3829105440777987
1104	98-99	0.39806146888940575
1104	100-101	0.5642965600973611
1105	1	0.017438648459481954
1105	2	-0.01664978100162884
1105	3	0.13665319699391176
1105	4	0.18633006580050449
1105	5	0.24349743836009452
1105	6	0.16325105589886846
1105	7	0.1505377014238789
1105	8	0.2504088178756163
1105	9	0.2691897033839723
1105	10-11	0.09487839668933873
1105	12-13	0.248155043377146
1105	14-15	0.25083307159781754
1105	16-17	0.3390784413156993
1105	18-19	0.13065791515406033
1105	20-21	0.44354192906336465
1105	22-23	0.2475942678999914
1105	24-25	0.3817415717022712
1105	26-27	0.247549965979168
1105	28-29	0.20401122306506636
1105	30-31	0.30150865840118257
1105	32-33	0.2640442950041404
1105	34-35	0.16034210219719114
1105	36-37	0.07093106042177766
1105	38-39	0.16939387455097688
1105	40-41	0.34145931493004156
1105	42-43	0.2729742035376077
1105	44-45	0.09648394695372531
1105	46-47	0.021138889701433072
1105	48-49	0.1034591386873629
1105	50-51	0.010713352427920597
1105	52-53	-0.12532632508189323
1105	54-55	-0.08599683724655449
1105	56-57	-0.06126334074829032
1105	58-59	-0.27124059099322295
1105	60-61	-0.11646860152499272
1105	62-63	-0.17163549839530035
1105	64-65	-0.2223894723411206
1105	66-67	-0.14614684068325445
1105	68-69	-0.14988957892883192
1105	70-71	0.012814912169925918
1105	72-73	0.08094317790809669
1105	74-75	0.07204367061008199
1105	76-77	0.17095995006797438
1105	78-79	-0.006471590030653118
1105	80-81	0.08829203172270539
1105	82-83	0.19419177750644678
1105	84-85	0.3613640633077573
1105	86-87	0.17954985139942536
1105	88-89	0.2471177082345548
1105	90-91	0.389283036345784
1105	92-93	0.359976594818221
1105	94-95	0.31762061238321593
1105	96-97	0.20893882000324382
1105	98-99	0.2967393256709556
1105	100-101	0.3868735418345999
1106	1	-0.19277774537757963
1106	2	-0.14808301655853384
1106	3	-0.07500165294798578
1106	4	-0.47273627066339685
1106	5	-0.23367272636394887
1106	6	-0.15255080979392943
1106	7	0.050639831761969845
1106	8	-0.023830330801878574
1106	9	-0.09983466240393568
1106	10-11	-0.1436553490320378
1106	12-13	0.12283310008518811
1106	14-15	0.0772838255606274
1106	16-17	0.07881221462222499
1106	18-19	-0.04085660262892077
1106	20-21	0.0754252340859054
1106	22-23	-0.27050447496490193
1106	24-25	-0.06775927106479429
1106	26-27	-0.7206813094516988
1106	28-29	-0.27108163966012455
1106	30-31	-0.15942522710669493
1106	32-33	-0.14867440031603962
1106	34-35	-0.19296783507848403
1106	36-37	-0.2489067943196872
1106	38-39	-0.24263111687855599
1106	40-41	0.0910940333672201
1106	42-43	0.033115158072710926
1106	44-45	-0.10320210229296123
1106	46-47	-0.5097923417341974
1106	48-49	-0.2690063920643766
1106	50-51	-0.2842949903673926
1106	52-53	-0.2284511109497629
1106	54-55	-0.20415185572113614
1106	56-57	-0.2623895403676997
1106	58-59	-0.27567008428492557
1106	60-61	-0.2983962225049197
1106	62-63	-0.40260513496389194
1106	64-65	-0.4313233442689288
1106	66-67	-0.35097084311646753
1106	68-69	-0.2941664891118734
1106	70-71	-0.24255356187303434
1106	72-73	-0.18160919602619074
1106	74-75	-0.17881788890214168
1106	76-77	-0.056253795890789604
1106	78-79	-0.04024876712405856
1106	80-81	-0.1647910239709809
1106	82-83	-0.017709907504183775
1106	84-85	0.08999286309383336
1106	86-87	0.09536672772251009
1106	88-89	0.16080023140851196
1106	90-91	0.279412628206952
1106	92-93	0.2147606131774218
1106	94-95	0.15827319590653843
1106	96-97	-0.08307606568531511
1106	98-99	0.06546326958033433
1106	100-101	0.21826815002760824
1107	1	-0.0928517140193037
1107	2	-0.2342608978537548
1107	3	-0.2288473532031965
1107	4	-0.034518971188759906
1107	5	-2.020533720058964
1107	6	-0.9666582420017136
1107	7	-0.6813321184854289
1107	8	-0.671271598522182
1107	9	-0.4733730899583861
1107	10-11	-0.36882697397089004
1107	12-13	-0.68248941130215
1107	14-15	-0.3819923124340221
1107	16-17	-0.3570699339324861
1107	18-19	-0.26755324084089693
1107	20-21	-0.38004362153908033
1107	22-23	-0.7699329057844224
1107	24-25	-0.4873650876228268
1107	26-27	-0.5884218741686027
1107	28-29	-0.7057978134510563
1107	30-31	-0.635104784725101
1107	32-33	-0.7895573450405493
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2308	48-49	-2.6993408936785777
2308	50-51	-2.0223380699179856
2308	52-53	-1.2186710820764617
2308	54-55	-0.9384934404461589
2308	56-57	-0.3405102474687425
2308	58-59	-0.9458117167651849
2308	60-61	-1.6176273790543725
2308	62-63	-0.5921437942108341
2308	64-65	-0.10797665532144762
2308	66-67	-0.689777014875002
2308	68-69	-0.5883789147009075
2308	70-71	-1.3606148754460392
2308	72-73	-0.5728160229994934
2308	74-75	-1.0269765098288168
2308	76-77	-1.79592738538814
2308	78-79	-1.7820470382544116
2308	80-81	-1.8119321643167687
2308	82-83	-1.2544425274903368
2308	84-85	-1.1644277765202844
2308	86-87	-1.1074918645916334
2308	88-89	-1.5732293410066376
2308	90-91	-1.6382199104935253
2308	92-93	-2.2057863218601703
2308	94-95	-1.4298140383592184
2308	96-97	-0.8647077785194206
2308	98-99	-1.0107575324164308
2308	100-101	-1.4844500101203018
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	210.0
11	1806.0
12	5296.0
13	10149.0
14	16642.0
15	23595.0
16	30753.0
17	42664.0
18	69207.0
19	131215.0
20	133690.0
21	121221.0
22	116065.0
23	115914.0
24	122544.0
25	134613.0
26	153251.0
27	166328.0
28	183599.0
29	200624.0
30	224027.0
31	257140.0
32	315226.0
33	419817.0
34	622284.0
35	1246139.0
36	1038011.0
37	1073895.0
38	563414.0
39	42104.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.489147080075057	48.68215391701495	12.498657910571866	13.330041092338126
2	15.3091316687995	20.59816431184381	52.552964058911066	11.539739960445623
3	14.990842135725273	44.665382481637444	16.809884425985384	23.533890956651895
4	25.003145831239408	17.364975251475244	43.67158999148185	13.960288925803493
5	14.94172232215897	27.756295553548902	15.57868390028203	41.723298224010094
6	15.722719443822314	17.312663441431216	41.33723812109063	25.627378993655842
7	24.771940395129647	18.038066805329475	15.677479578144634	41.51251322139625
8	51.36763859002394	17.54544176307947	15.53979957955339	15.547120067343212
9	15.554229514043596	43.46513904574078	16.023096864430634	24.957534575784987
10-11	46.766791154529294	17.231597282951494	15.801338844671383	20.200272717847824
12-13	33.214761192780735	17.121358081335384	16.253708635690973	33.410172090192916
14-15	32.608103527189606	17.167168553523826	16.56855141339517	33.6561765058914
16-17	32.83790868148049	22.11678080662716	16.215027853993096	28.830282657899254
18-19	28.11838845220797	17.546619161129453	21.478435736151166	32.85655665051141
20-21	27.298773480977793	34.804290890826245	18.004134034412385	19.892801593783567
22-23	13.844627044840038	21.638936083691522	36.047359473325784	28.469077398142655
24-25	13.775152958438555	17.473393234841712	40.91162972170207	27.83982408501766
26-27	13.65982558293292	17.313333752339847	54.555696682635734	14.471143982091494
28-29	13.830273936058834	16.95431826942767	54.931742690768125	14.283665103745372
30-31	13.85615751616511	16.878158477253688	55.03532059875581	14.230363407825392
32-33	13.658528316673493	16.883702011455455	55.12648510118618	14.331284570684883
34-35	13.887248826409984	17.005895782779394	54.91290608694459	14.193949303866027
36-37	13.990259440050364	16.863392227891588	54.8083050257021	14.338043306355953
38-39	13.970313704165255	17.143946316550714	54.116881999101054	14.768857980182975
40-41	14.836212349536149	18.549827670568675	50.843734577523314	15.77022540237186
42-43	14.970514291201656	19.36899072825773	50.21782285833795	15.44267212220267
44-45	15.050931302430445	20.726366298043157	48.96274843041732	15.259953969109072
46-47	14.823712880817434	21.9540821249332	48.32912442451627	14.893080569733092
48-49	14.649859090502598	22.356862040593224	48.03653305919096	14.956745809713217
50-51	14.49515564975904	22.6690622473071	47.806296689140574	15.029485413793292
52-53	14.36507892541043	23.117018854674733	47.50194619454665	15.015956025368194
54-55	14.48656604755125	23.008998792053564	47.261544013542505	15.242891146852678
56-57	14.394802743850724	22.962154265232748	47.267360833570095	15.375682157346432
58-59	14.457574277889936	23.226186453219707	46.78811949463045	15.528119774259899
60-61	14.683645449914543	23.176954524074645	46.265225625347725	15.87417440066309
62-63	14.919602577972427	23.447951068013005	45.76856680149554	15.863879552519025
64-65	14.917303862494569	23.67930954728314	45.31785135594916	16.08553523427313
66-67	15.100137557842066	23.60318437664795	44.78076657083899	16.51591149467099
68-69	15.23585870475245	23.866963951028154	44.26276382772326	16.634413516496135
70-71	15.671338230807919	23.922036162160826	43.96907170595365	16.43755390107761
72-73	15.82546356294662	23.932009668622158	43.742528417241274	16.499998351189944
74-75	15.491477765106518	24.252479271148495	43.21663156789548	17.03941139584951
76-77	16.54379074414086	23.748140734501142	43.203232402861275	16.504836118496726
78-79	15.818927382079922	24.541400758380732	42.42962564655859	17.210046212980753
80-81	16.231979745486218	24.178288266688387	42.52292302684541	17.066808960979984
82-83	16.73139928638915	24.1394349334289	42.20112187086284	16.92804390931911
84-85	16.600351166246412	23.99421894067245	41.99800839155821	17.407421501522926
86-87	16.8212740497809	24.076787564192763	41.74173793919959	17.360200446826745
88-89	17.116570203022118	24.06321886079689	41.77262498944791	17.047585946733072
90-91	17.265755144435005	24.282606406015862	40.95256950857692	17.499068940972208
92-93	17.759341961247983	24.209655213166684	40.71740743642565	17.31359538915968
94-95	17.82997269784565	24.539082415995985	40.277965186884366	17.352979699274005
96-97	17.807648112762877	24.701925201947592	40.06015806793517	17.430268617354365
98-99	18.121819466905375	24.67839439932693	39.72565924556293	17.474126888204772
100-101	18.58930696443877	24.53678434939559	39.17989125574527	17.694017430420374
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	118724.0
1	69174.5
2	13498.5
3	5902.5
4	4001.0
5	3383.5
6	3180.0
7	3105.5
8	3062.0
9	3289.0
10	4067.0
11	5422.0
12	9774.5
13	15347.0
14	20681.0
15	41069.0
16	768136.0
17	839477.5
18	161634.5
19	103960.5
20	72132.0
21	51972.5
22	42653.5
23	39836.0
24	40048.5
25	41898.0
26	44108.0
27	46475.0
28	51261.5
29	56237.0
30	61005.0
31	66812.5
32	73908.5
33	82782.0
34	91928.0
35	100976.5
36	111748.5
37	122061.0
38	130147.0
39	137636.5
40	142436.0
41	144767.5
42	148788.0
43	154245.0
44	160322.0
45	170690.0
46	183759.5
47	195289.5
48	205240.5
49	214512.5
50	211267.5
51	202238.0
52	201681.0
53	204588.0
54	199775.0
55	187170.0
56	173663.5
57	158962.0
58	140463.0
59	121633.5
60	105819.5
61	89818.5
62	73626.5
63	55839.0
64	39003.0
65	25857.0
66	17208.0
67	12426.0
68	9298.5
69	6985.5
70	5366.5
71	3884.0
72	3011.0
73	2346.0
74	1815.5
75	1606.0
76	1295.0
77	758.5
78	404.5
79	218.0
80	102.0
81	51.0
82	35.0
83	21.0
84	8.5
85	3.0
86	1.0
87	1.0
88	1.5
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.3190068089769494E-5
8	0.0
9	0.0
10-11	0.0
12-13	1.978510213465424E-5
14-15	9.496849024634035E-4
16-17	7.056686428026679E-4
18-19	3.9570204269308485E-4
20-21	7.914040853861696E-5
22-23	1.3190068089769494E-5
24-25	9.562799365082882E-4
26-27	4.814374852765865E-4
28-29	5.21007689545895E-4
30-31	0.0062652823426405085
32-33	0.009331973173511915
34-35	0.005935530640396272
36-37	0.0022818817795301222
38-39	0.004675879137823285
40-41	0.0026050384477294746
42-43	0.005493663359388994
44-45	0.006146571729832583
46-47	0.001615783340996763
48-49	0.0
50-51	1.978510213465424E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	1.978510213465424E-5
66-67	0.013381324077071152
68-69	0.014054017549649394
70-71	0.013513224757968846
72-73	0.00312604613727537
74-75	0.0
76-77	0.011178582706079646
78-79	0.0162567589206409
80-81	0.012260168289440744
82-83	2.506112937056204E-4
84-85	0.0020114853836898476
86-87	0.0
88-89	2.9018149797492885E-4
90-91	5.47387825725434E-4
92-93	3.1656163415446785E-4
94-95	1.1871061280792544E-4
96-97	0.0013387919111116035
98-99	0.0011541309578548306
100-101	5.2760272359077975E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	7581462.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.91743369461157
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.5734185987637	25.864352968837633
2	10.884681992317466	7.165915955380855
3	3.2328630337655957	3.1925266367321936
4	1.544229821596102	2.0332833104652615
5	0.9106529713517239	1.4988179401635688
6	0.6364258352200112	1.2569703139435502
7	0.4937902275635371	1.1378014952408924
8	0.36923153229510736	0.972332358582722
9	0.33389800207793535	0.9891958809747341
>10	2.8355975451015545	18.358056385642453
>50	0.15038240293343744	3.2172429754668297
>100	0.02647512624553684	1.8088073781210332
>500	0.004334062403718652	1.014550758925203
>1k	0.003033836510633014	2.1526335024469474
>5k	4.7280568996451095E-4	1.1595622890129682
>10k+	5.122061641282202E-4	28.17794985006324
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	1755905	23.1605065091667	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	112331	1.481653538591897	No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT	79608	1.0500349404903697	No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48881	0.6447437182960226	No Hit
GTCGACGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44525	0.5872877816969867	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32837	0.4331222658637608	No Hit
CTATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	19135	0.25239195289773925	No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT	16793	0.2215008134314991	No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15292	0.2017025212287551	No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15182	0.20025161373888045	No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTAAAAAAAAAA	10853	0.14315180897826832	No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10771	0.1420702233949072	No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTAAAAAAAAA	10498	0.13846933480640014	No Hit
ATATCTCGAGGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTT	9780	0.12899886591794563	No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTAAAAAAAA	9197	0.12130905622161003	No Hit
ATATCTCGAGGGCGCGCCGTATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	9168	0.1209265442470067	No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTAAAAAAAAAAA	8699	0.11474040231290482	No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTAAAAAAA	8145	0.10743310459117252	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.561318384237763E-4	0.0	0.0	0.0	0.0
2	3.561318384237763E-4	0.0	0.0	0.0	0.0
3	3.561318384237763E-4	0.0	0.0	0.0	0.0
4	3.561318384237763E-4	0.0	0.0	0.0	0.0
5	3.825119746033153E-4	0.0	0.0	0.0	0.0
6	3.825119746033153E-4	0.0	0.0	0.0	1.3190068089769494E-5
7	3.825119746033153E-4	0.0	0.0	0.0	1.3190068089769494E-5
8	3.957020426930848E-4	0.0	0.0	0.0	1.3190068089769494E-5
9	3.957020426930848E-4	0.0	0.0	0.0	1.3190068089769494E-5
10-11	4.088921107828543E-4	0.0	0.0	0.0	1.978510213465424E-5
12-13	4.41867281007278E-4	0.0	0.0	0.0	3.957020426930848E-5
14-15	5.012225874112407E-4	0.0	0.0	1.3190068089769494E-5	3.957020426930848E-5
16-17	5.144126555010102E-4	0.0	0.0	1.3190068089769494E-5	3.957020426930848E-5
18-19	5.605778938152035E-4	0.0	0.0	1.3190068089769494E-5	4.6165238314193225E-5
20-21	6.133381661742814E-4	0.0	0.0	1.3190068089769494E-5	5.2760272359077975E-5
22-23	7.056686428026679E-4	0.0	0.0	1.3190068089769494E-5	5.935530640396272E-5
24-25	8.243792556105933E-4	0.0	0.0	1.3190068089769494E-5	7.914040853861696E-5
26-27	0.001094775651450868	0.0	0.0	1.3190068089769494E-5	7.914040853861696E-5
28-29	0.001510262796278607	0.0	0.0	1.3190068089769494E-5	7.914040853861696E-5
30-31	0.002156576132677312	0.0	0.0	1.3190068089769494E-5	9.233047662838645E-5
32-33	0.0029413851840185967	0.0	0.0	1.3190068089769494E-5	1.0552054471815595E-4
34-35	0.0038317147800780377	0.0	0.0	1.3190068089769494E-5	1.0552054471815595E-4
36-37	0.005051796078381716	0.0	0.0	1.3190068089769494E-5	1.0552054471815595E-4
38-39	0.006416968125672859	0.0	0.0	1.3190068089769494E-5	1.121155787630407E-4
40-41	0.00755790901543792	0.0	0.0	3.957020426930848E-5	1.1871061280792544E-4
42-43	0.008632899564754134	0.0	0.0	3.957020426930848E-5	1.3190068089769492E-4
44-45	0.010281658075975319	0.0	0.0	3.957020426930848E-5	1.4509074898746442E-4
46-47	0.011864466246747658	0.0	0.0	3.957020426930848E-5	1.5828081707723393E-4
48-49	0.013631935370776772	0.0	0.0	3.957020426930848E-5	1.5828081707723393E-4
50-51	0.01572915619705012	0.0	0.0	3.957020426930848E-5	1.5828081707723393E-4
52-53	0.01964660641971166	0.0	0.0	3.957020426930848E-5	1.5828081707723393E-4
54-55	0.02505453433651715	0.0	0.0	3.957020426930848E-5	1.714708851670034E-4
56-57	0.03006676021062956	0.0	0.0	3.957020426930848E-5	1.714708851670034E-4
58-59	0.03465690390586934	0.0	0.0	3.957020426930848E-5	1.7806591921188815E-4
60-61	0.04022311263975207	0.0	0.0	3.957020426930848E-5	1.9125598730165765E-4
62-63	0.04549254484161498	0.0	0.0	3.957020426930848E-5	2.110410894363119E-4
64-65	0.05153359602672941	0.0	0.0	3.957020426930848E-5	2.5720632775050507E-4
66-67	0.05983014885519442	0.0	0.0	3.957020426930848E-5	2.6380136179538985E-4
68-69	0.06780354501546007	0.0	0.0	3.957020426930848E-5	2.9018149797492885E-4
70-71	0.10725503867196062	0.0	0.0	4.6165238314193225E-5	2.9018149797492885E-4
72-73	0.14519626953218256	0.0	0.0	5.2760272359077975E-5	2.9018149797492885E-4
74-75	0.15671119897455132	0.0	0.0	5.2760272359077975E-5	3.0337156606469835E-4
76-77	0.16969022597488453	0.0	0.0	5.2760272359077975E-5	3.1656163415446785E-4
78-79	0.18393549951183558	0.0	0.0	5.2760272359077975E-5	3.2975170224423735E-4
80-81	0.20064731578157352	0.0	0.0	5.2760272359077975E-5	3.429417703340068E-4
82-83	0.22026754206510565	0.0	0.0	5.2760272359077975E-5	3.495368043788915E-4
84-85	0.2433237810860227	0.0	0.0	5.2760272359077975E-5	3.561318384237763E-4
86-87	0.270429371010499	0.0	0.0	5.2760272359077975E-5	3.561318384237763E-4
88-89	0.3093005016710497	0.0	0.0	5.2760272359077975E-5	3.561318384237763E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	72630	0.0	94.50475	1
ATATCTC	203345	0.0	94.215546	1
TATCTCG	206405	0.0	93.64946	2
TCGACGG	75635	0.0	90.737495	2
ATCTCGA	213230	0.0	90.58735	3
TCTCGAG	211885	0.0	90.25457	4
CTCGAGG	213490	0.0	89.286835	5
TCGAGGG	212565	0.0	89.21958	6
CGAGGGC	213120	0.0	88.77329	7
ACGGCGC	76915	0.0	88.70876	5
CGACGGC	77020	0.0	88.6495	3
GACGGCG	77080	0.0	88.59281	4
AGGGCGC	216045	0.0	87.59073	9
GAGGGCG	216425	0.0	87.49664	8
CGGCGCG	77695	0.0	87.38418	6
CTATCTC	4050	0.0	55.937763	1
CGCGCCT	2335	0.0	50.03784	9
TTCGAAC	1690	0.0	49.742577	3
TCTCGAC	3600	0.0	48.022038	4
GTCGACT	950	0.0	47.994267	1
>>END_MODULE